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This paper deals with the study of construction and geometry, as well as with the analysis of deformations and active degradations of the natatio belonging to the Western Baths in La Alcudia archaeological site. Its location in Elche-Alicante, Spain (Colonia Iulia Ilici Augusta), is widely known for the discovery of The Lady of Elche in 1897. The dimensions of this natatio in the frigidarium are 6.60 x 9.30 m (22 x 31 Roman feet) and 1.50 m deep, making it one of the largest Roman swimming pools documented to date on the Iberian Peninsula. The natatio has several cracks sealed with materials used in earlier interventions at the end of the third century. Its comparison with the hypothetical original form allowed the researchers to quantify the current deformations. A damage evolution study has been made comparing the 2016 point cloud with the 2022 cloud, both obtained by light detection and ranging (LIDAR). There is evidence that an active process of degradation and deformation is gradually increasing damage to the pool. By studying the geometry and constructive systems of the natatio, relevant data to understand the historical evolution of the Western Baths have been provided. A results analysis conclusion is that the pool was built in two different stages. The eastern half corresponds to the original natatio, while the western half was extended or rebuilt after having collapsed. The early abandonment of the use of the natatio was most likely due to deformations caused by differential settlement; this occurred when the western half was cemented on landfill between the ancient wall and that of the last third of the 1st century AD.

This work provides new data on the church of Nuestra Señora de la Asunción in Biar, combining the investigation of documentary sources with the analysis of the geometry obtained using LiDAR technology and the study of stone materials. This monument has a Proto-Renaissance façade of great architectural value, as it was the first building in the province of Alicante, in Spain, to use Renaissance decorations at the beginning of the 16th century. Its main façade reflects the four centuries of its construction from the 15th to the 18th centuries. The building has been digitised using LiDAR technology and photogrammetry. The graphic representation of the point cloud obtained from the aforementioned techniques makes it possible to study deformations in colour gradient with respect to a reference plane. The results obtained after the analysis of these data show that the method used in this work has served to detect and corroborate the constructive evolutions of the church obtained from the documentary sources investigated. This work serves as an example for similar works, proposing the incorporation of the analysis of anomalies in the geometry of the facings as a new variable that should be considered to complement the rest of the usual studies, bringing to light deformations and irregularities that at first sight may go unnoticed.

Abstract Antigen cognate dendritic cell (DC)-T cell synaptic interactions drive activation of T cells and instruct DCs. Upon receiving CD4+ T cell help, post-synaptic DCs (psDCs) are licensed to generate CD8+ T cell responses. However, the cellular and molecular mechanisms that enable psDCs licensing remain unclear. Here, we describe that antigen presentation induces an upregulation of MHC-I protein molecules and increased lipid peroxidation on psDCs in vitro and in vivo. We also show that these events mediate DC licensing. In addition, psDC adoptive transfer enhances pathogen-specific CD8+ T responses and protects mice from infection in a CD8+ T cell-dependent manner. Conversely, depletion of psDCs in vivo abrogates antigen-specific CD8+ T cell responses during immunization. Together, our data show that psDCs enable CD8+ T cell responses in vivo during vaccination and reveal crucial molecular events underlying psDC licensing.

Marfan syndrome (MFS) is a multisystem genetic disorder with over 3000 mutations described in the fibrillin 1 (FBN1) gene. Like MFS, other connective tissue disorders also require a deeper understanding of the phenotype-genotype relationship due to the complexity of the clinical presentation, where diagnostic criteria often overlap. Our objective was to identify mutations in patients with connective tissue disorders using a genetic multipanel and to analyze the genotype-phenotype associations in a cohort of Mexican patients. We recruited 136 patients with MFS and related syndromes from the National Institute of Cardiology. Mutations were identified using next-generation sequencing (NGS). To examine the correlation between mutation severity and severe cardiovascular conditions, we focused on patients who had undergone Bentall-de Bono surgery or aortic valve repair. The genetic data obtained allowed us to reclassify the initial clinical diagnosis across various types of connective tissue disorders. The transforming growth factor beta receptor 2 (TGFBR2) rs79375991 mutation was found in 10 out of 16 (63%) Loeys-Dietz patients. We observed a high prevalence (65%) of more severe mutations, such as frameshift indels and stop codons, among patients requiring invasive treatments like aortic valve-sparing surgery, Bentall and de Bono procedures, or aortic valve replacement due to severe cardiovascular injury. Although our study did not achieve precise phenotype-genotype correlations, it underscores the importance of a multigenetic panel evaluation. This could pave the way for a more comprehensive diagnostic approach and inform medical and surgical treatment decision-making.

Background. A global approach in the development of diets low in crude protein (CP) supplemented with amino acids is to obtain productive yields similar to standard diets. Objective. To evaluate the reduction of CP in diets with similar amounts of Methionine, Lysine, Threonine, Valine, Arginine, and Tryptophan and to measure its effect on the productive performance, carcass yield and intestinal integrity. Methodology. 882 mixed Ross chickens from 1 to 49 days were used in a 2X3 factorial arrangement; one factor was sex (male and female) and another 3 PC levels (0, 1 and 2% less), each treatment had 6 replicates of 25 chickens each. Results. The results indicated similar (P>0.05) productive and carcass performance with the different protein levels. Females showed a higher percentage of abdominal fat compared to males (P0.05) in the sex factor or the protein factor. Implications. PC reduction lowers costs and reduces nitrogen pollution. Conclusion. It is concluded that diets reduced in CP supplemented with amino acids do not affect productivity, carcass performance and intestinal integrity.

ABSTRACT Objective: Recent studies have shown a relationship between adipose tissue and coronary artery disease (CAD). The ABCA1 transporter regulates cellular cholesterol content and reverses cholesterol transport. The aim of this study was to determine the relationship between single nucleotide polymorphisms (SNPs) R230C, C-17G, and C-69T and their expression in epicardial and mediastinal adipose tissue in Mexican patients with CAD. Subjects and methods: The study included 71 patients with CAD and a control group consisting of 64 patients who underwent heart valve replacement. SNPs were determined using TaqMan probes. mRNA was extracted using TriPure Isolation from epicardial and mediastinal adipose tissue. Quantification and expression analyses were done using RT-qPCR. Results: R230C showed a higher frequency of the GG genotype in the CAD group (70.4%) than the control group (57.8%) [OR 0.34, 95% CI (0.14-0.82) p = 0.014]. Similarly, C-17G (rs2740483) showed a statistically significant difference in the CC genotype in the CAD group (63.3%) in comparison to the controls (28.1%) [OR 4.42, 95% CI (2.13-9.16), p = 0.001]. mRNA expression in SNP R230C showed statistically significant overexpression in the AA genotype compared to the GG genotype in CAD patients [11.01 (4.31-15.24) vs. 3.86 (2.47-12.50), p = 0.015]. Conclusion: The results suggest that the GG genotype of R230C and CC genotype of C-17G are strongly associated with the development of CAD in Mexican patients. In addition, under-expression of mRNA in the GG genotype in R230C is associated with patients undergoing revascularization.

Abstract Background Takayasu’s arteritis (TA) is a chronic inflammatory disease affecting the large arteries and their branches; its etiology is still unknown. In individuals suffering from TA, arterial inflammation progresses to stenosis and/or occlusion, leading to organ damage and affecting survival. Relation of TA with Mycobacterium tuberculosis has been known, but there have been only a few systematic studies focusing on this association. The IS6110 sequence identifies the Mycobacterium tuberculosis complex and the HupB establishes the differences between M. tuberculosis and M. bovis. Our objective was to search the presence of IS6110 and HupB genes in aorta of patients with TA. Methods We analyzed aorta tissues embedded in paraffin from 5760 autopsies obtained from our institution, we divided the selected samples as cases and controls; Cases: aortic tissues of individuals with Takayasu’s arteritis. Control positive: aortic tissues (with tuberculosis disease confirmed) and control negative with other disease aortic (atherosclerosis). Results Of 181 selected aorta tissues, 119 fulfilled the corresponding criteria for TA, TB or atherosclerosis. Thus 33 corresponded to TA, 33 to tuberculosis (TB) and 53 to atherosclerosis. The mean age was 22 ± 13, 41 ± 19, and 57 ± 10, respectively. IS6110 and HupB sequences were detected in 70% of TA tissues, 82% in tuberculosis, and in 32% with atherosclerosis. Important statistical differences between groups with TA, tuberculosis versus atherosclerosis (p = 0.004 and 0.0001, respectively) were found. Conclusion We identified a higher frequency of IS6110 and HupB genes in aortic tissues of TA patients. This data suggests that arterial damage could occur due to previous infection with M. tuberculosis.

From 2011 to 2016, California experienced a millennial-intensity drought, generating high levels of tree mortality. Remote sensing has been used to monitor the long-term impacts of drought; however, discriminating dead from live trees in arid and semiarid deciduous woodlands is challenging. The goals of this study were to assess and map the spatial patterns of drought-induced tree mortality in a blue oak (Quercus douglasii) woodland, a highly drought-tolerant species forming savannas along the lower foothills surrounding California's Central Valley. Airborne hyperspectral imagery was used to identify the most important wavelength regions predicting drought-induced blue oak mortality. The best metric to predict canopy stress was a normalized ratio using the spectral bands 937.53 and 1100.08 nm with a correlation with tree mortality of R2 = 0.83. The image prediction of mortality for nine field plots found that 16 of 98 trees died (17.9%) during the drought. We further evaluated tree mortality in 82 independent plots, and we found the greatest image predictive accuracy for tree mortality between 1% and 10%. When applied at the landscape level, the regression-based index found mortality ranged from 1% to more than 51% in oak stands with an average mortality of 10% over the entire study region. In addition, tree mortality at landscape level showed higher tree mortality in blue oaks on south-facing aspects probably because of higher insolation rates and in sites with low potential for accumulated drainage.

Low-density lipoprotein cholesterol (LDL-C), which makes up about 70% of the cholesterol in the blood, is critical in the formation of arteriosclerotic plaques, increasing the risk of heart disease. LDL-C levels are estimated using Friedewald, Martin and Sampson equations, though they have limitations with high triglycerides. Our aim is to compare the effectiveness of these equations versus the ultracentrifugation technique in individuals with and without dyslipidemia and identify precision. There were 113 participants, 59 healthy controls and 54 dyslipidemic patients. Samples were collected after fasting. LDL-C was estimated using the Friedewald, Martin and Sampson equations. The purified LDL-C, ultracentrifugated and dialysized control group without dyslipidemia vs. patients with coronary artery disease (CAD) showed differences in age, HDL-C, triglycerides and glucose non-HDL-C (p = 0.001 in all). There were correlations in CGWD between ultracentrifugation and Sampson R-squared (R2) = 0.791. In the dyslipidemia control group, ultracentrifugation and Friedewald R2 = 0.911. In patients with CAD, correlation between ultracentrifugation and Sampson R2 = 0.892; Bland–Altman confirmed agreement in controls without dyslipidemia. The Martin and Sampson equations are interchangeable with ultracentrifugation. Conclusion: The role of LDL analysis using precise techniques is necessary to obtain better control of disease outcomes after the use of precise therapies and suggests verifying its importance through clinical trials.

In this study, we evaluate how ChatGPT complements and enriches the traditional flipped learning strategy in higher education, particularly in engineering courses. Using an experimental design involving 356 students from basic programming courses in undergraduate engineering programs, we compared the normalized learning gain between groups that used the ChatGPT-assisted flipped learning strategy (focus groups) and those that followed a traditional video-based flipped learning methodology (control groups). The intervention lasted ten weeks, with two sessions of two hours each week. A pre-test–post-test analysis revealed that the focus groups showed significant improvement in normalized learning gain values compared to the control groups. These results confirm that incorporating ChatGPT into the flipped learning strategy can significantly enhance student performance by providing a more active, interactive, and personalized approach during the teaching–learning process. We conclude that the flipped learning strategy, upgraded with the assistance of ChatGPT, provides an effective means to improve understanding and application of complex concepts in programming courses, with potential to be extended to other areas of study in higher education. This study opens routes for future research on the integration of artificial intelligence into innovative pedagogical strategies with the goal of scaffolding the learning experience and improving educational outcomes.

Este trabajo describe los estudios realizados y la intervención llevada a cabo para la restauración de la portada de la fachada de San Pedro Apóstol en la Iglesia parroquial de Agost. Esta portada se realizó a finales del siglo XVIII en piedra calcárea. Tras una primera inspección y levantamiento gráfico de la misma se identificaron las lesiones, su origen y la solución a las mismas, empleando técnicas avanzadas de restauración. Los elementos de piedra dañados o destruidos por el paso del tiempo están compuestos por calcita, dolomita y sílice, con juntas de yeso, y se han reconstruido bien mediante el uso de nuevos elementos o, en su mayor parte, mediante sistemas de recuperación del volumen inicial con la utilización de un núcleo ligero de poliestireno, morteros de cal, arcilla expandida, fibra de vidrio y carbono junto con infiltraciones de resinas en las zonas disgregadas. Tras un proceso delicado proceso de intervención con técnicas innovadoras la fachada ha finalizado su restauración en junio de 2023.

The mountain region of central Veracruz, Mexico hosts a large system of karst and volcanic caves that are unexplored. In particular, the vertebrates that inhabit these subterranean ecosystems are unknown. This study evaluated the diversity of mammals, birds, reptiles, amphibians, and fish in three environments (euphotic, disphotic, and aphotic) of 16 caves of different geological origin (12 karst caves and 4 volcanic caves) distributed along an altitudinal gradient (300-2400 m a.s.l.). We found a richness of 242 vertebrate species (184 birds, 30 mammals, 15 reptiles, 12 amphibians, and 1 fish) and an abundance of a total of 11,323 individuals (4,969 mammals, 6,483 birds, 36 reptiles, 27 amphibians, and 5 fish). The richness of all vertebrate classes was higher in karst than in volcanic caves. Vertebrate diversity was also higher at mid-altitudes between 600-899 m a.s.l. Diversity varied between environments, where bird and reptile richness was higher in the euphotic environment, while mammal and amphibian diversity was higher in the aphotic environment. The similarity in the composition of vertebrate species does not depend on the distance between karstic and volcanic caves. Volcanic and karst caves shared on average up to 70% and 55% of vertebrate species, which indicates that only 30% and 45% of species, respectively, is different in each cave type. Given the vulnerability and fragility of these subterranean ecosystems, as well as the important diversity that they contain, we recommend including the caves of the central region of Veracruz in the conservation agenda of local governments and communities. Community-based conservation can help ensure the presence of vertebrate species in the caves of this region.

Marfan syndrome (MFS) is an inherited connective tissue disorder. As the spinal growth depends on delicate balance of forces, conditions that affect musculoskeletal matrix often lead to spinal deformities. A large cross-sectional study revealed a 63% prevalence of scoliosis among patients with MFS. Multi-ethnic genome-wide association studies and analyses of human genetic mutations showed that variations and mutations of G protein-coupled receptor 126 (GPR126)locus are associated with multiple skeletal defects, including shorter stature and adolescent idiopathic scoliosis. The study included 54 patients with MFS and 196 control patients. The DNA was extracted from peripheral blood using the saline expulsion method and single nucleotide polymorphism (SNP) determination was carried out using TaqMan probes. Allelic discrimination was performed by RT-qPCR. Significant differences in genotype frequencies were found for SNP rs6570507 in relation to MFS and sex (recessive model, OR 2.46, 95% CI 1.03 -5.87; P = 0.03) and rs7755109 (overdominant model, OR 0.39, 95% CI 0.16-0.91; P = 0.03). The most significant association was found in SNP rs7755109, where the frequency of genotype AG was significantly different between MFS patients with scoliosis and those without (OR 5.68, 95% CI 1.09-29.48; P=0.04). This study, for the first time, examined the genetic association of SNP GPR126 with the risk of scoliosis in patients with connective tissue diseases. The study revealed that SNP rs7755109 is associated with the presence of scoliosis in Mexican patients with MFS.