12 results on '"Huber FJ"'
Search Results
2. Microbial desulphurization of coal. - Report 3
- Author
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Boogerd, Fred C., Bos, P, Huber, FJ, Molecular Cell Physiology, and AIMMS
- Published
- 1989
3. Th17 Cells Express Interleukin-10 Receptor and Are Controlled by Foxp3− and Foxp3+ Regulatory CD4+ T Cells in an Interleukin-10-Dependent Manner
- Author
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Yasushi Kobayashi, Carmen J. Booth, William T. O'Connor, Ashutosh Chaudhry, Maria Grazia Roncarolo, Francis J. Huber, Enric Esplugues, Samuel Huber, Nicola Gagliani, Manuela Battaglia, Alexander Y. Rudensky, Masahito Kamanaka, Richard A. Flavell, Huber, S, Gagliani, N, Esplugues, E, O'Connor, W, Huber, Fj, Chaudhry, A, Kamanaka, M, Kobayashi, Y, Booth, Cj, Rudensky, Ay, Roncarolo, MARIA GRAZIA, Battaglia, MARCO MARIA, and Flavell, Ra
- Subjects
CD4-Positive T-Lymphocytes ,Interleukin-10 Receptor alpha Subunit ,Cell ,Immunology ,chemical and pharmacologic phenomena ,Biology ,Article ,Interleukin 10 receptor, alpha subunit ,Interferon-gamma ,Mice ,03 medical and health sciences ,Interleukin 21 ,0302 clinical medicine ,medicine ,Animals ,Immunology and Allergy ,Interferon gamma ,Receptor ,Cell Proliferation ,030304 developmental biology ,0303 health sciences ,FOXP3 ,Forkhead Transcription Factors ,hemic and immune systems ,Colitis ,Molecular biology ,Peptide Fragments ,Interleukin-10 ,3. Good health ,Mice, Inbred C57BL ,Interleukin 10 ,medicine.anatomical_structure ,Infectious Diseases ,Disease Progression ,Th17 Cells ,Signal transduction ,Signal Transduction ,030215 immunology ,medicine.drug - Abstract
T helper 17 (Th17) cells are important for host defense against extracellular microorganisms. However, they are also implicated in autoimmune and chronic inflammatory diseases, and as such need to be tightly regulated. The mechanisms that directly control committed pathogenic Th17 cells in vivo remain unclear. We showed here that IL-17A-producing CD4(+) T cells expressed interleukin-10 receptor alpha (IL-10R alpha) in vivo. Importantly, T cell-specific blockade of IL-10 signaling led to a selective increase of IL-17A(+)IFN-gamma(-) (Th17) and IL-17A(+)IFN-gamma(+) (Th17+Th1) CD4(+) T cells during intestinal inflammation in the small intestine. CD4(+)Foxp3(-) IL-10-producing (Tr1) cells and CD4(+)Foxp3(+) regulatory (Treg) cells were able to control Th17 and Th17+Th1 cells in an IL-10-dependent manner in vivo. Lastly, IL-10 treatment of mice with established colitis decreased Th17 and Th17+Th1 cell frequencies via direct signaling in T cells. Thus, IL-10 signaling directly suppresses Th17 and Th17+Th1 cells.
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4. Tissue resident iNKT17 cells facilitate cancer cell extravasation in liver metastasis via interleukin-22.
- Author
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Giannou AD, Kempski J, Shiri AM, Lücke J, Zhang T, Zhao L, Zazara DE, Cortesi F, Riecken K, Amezcua Vesely MC, Low JS, Xu H, Kaffe E, Garcia-Perez L, Agalioti T, Yamada Y, Jungraithmayr W, Zigmond E, Karstens KF, Steglich B, Wagner J, Konczalla L, Carambia A, Schulze K, von Felden J, May P, Briukhovetska D, Bedke T, Brockmann L, Starzonek S, Lange T, Koch C, Riethdorf S, Pelczar P, Böttcher M, Sabihi M, Huber FJ, Reeh M, Grass JK, Wahib R, Seese H, Stüben BO, Fard-Aghaie M, Duprée A, Scognamiglio P, Plitzko G, Meiners J, Soukou S, Wittek A, Manthey C, Maroulis IC, Arck PC, Perez D, Gao B, Zarogiannis SG, Strowig T, Pasqualini R, Arap W, Gosálvez JS, Kobold S, Prinz I, Guse AH, Tachezy M, Ghadban T, Heumann A, Li J, Melling N, Mann O, Izbicki JR, Pantel K, Schumacher U, Lohse AW, Flavell RA, Gagliani N, and Huber S
- Subjects
- Animals, Mice, Endothelial Cells metabolism, Mice, Inbred C57BL, Colorectal Neoplasms metabolism, Interleukin-22, Interleukins metabolism, Liver Neoplasms pathology, Liver Neoplasms secondary, Natural Killer T-Cells metabolism
- Abstract
During metastasis, cancer cells invade, intravasate, enter the circulation, extravasate, and colonize target organs. Here, we examined the role of interleukin (IL)-22 in metastasis. Immune cell-derived IL-22 acts on epithelial tissues, promoting regeneration and healing upon tissue damage, but it is also associated with malignancy. Il22-deficient mice and mice treated with an IL-22 antibody were protected from colon-cancer-derived liver and lung metastasis formation, while overexpression of IL-22 promoted metastasis. Mechanistically, IL-22 acted on endothelial cells, promoting endothelial permeability and cancer cell transmigration via induction of endothelial aminopeptidase N. Multi-parameter flow cytometry and single-cell sequencing of immune cells isolated during cancer cell extravasation into the liver revealed iNKT17 cells as source of IL-22. iNKT-cell-deficient mice exhibited reduced metastases, which was reversed by injection of wild type, but not Il22-deficient, invariant natural killer T (iNKT) cells. IL-22-producing iNKT cells promoting metastasis were tissue resident, as demonstrated by parabiosis. Thus, IL-22 may present a therapeutic target for prevention of metastasis., Competing Interests: Declaration of interests S.K. declares honoraria from GSK, BMS, Novartis, and TCR2, Inc.; license fees from TCR2, Inc. and Carina Biotech; and research support from TCR2, Inc., Plectonic GmbH, Tabby Therapeutics, and Arcus Biosciences., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2023
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5. 4D temperature measurements using tomographic two-color pyrometry.
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Yu T, Bauer FJ, Huber FJ, Will S, and Cai W
- Abstract
This work presents a new approach for high-speed four-dimensional (3D + t) thermometry using only two high-speed cameras which are equipped with different band pass filters to capture thermal radiation signals at two narrow wavelength bands. With the help of a customized fiber bundle and a beam splitter, a total number of nine projections at each band were recorded, and the temperature distribution was evaluated by tomographic two-color pyrometry. In order to validate the effectiveness of this method, the 3D temperature distribution of a premixed steady flat flame was evaluated. The determined temperatures were compared to those of other studies, as well as to the results from inverse Abel transform and line-of-sight data. Further, the 3D temperature evolution of a weakly turbulent diffusion flame was observed at a repetition rate of 7.5 kHz. Such 4D temperature measurements are expected to be valuable in understanding turbulent combustion mechanisms especially of practical devices.
- Published
- 2021
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6. Colitis Promotes a Pathological Condition of the Liver in the Absence of Foxp3 + Regulatory T Cells.
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Mathies F, Steffens N, Kleinschmidt D, Stuhlmann F, Huber FJ, Roy U, Meyer T, Luetgehetmann M, von Petersdorff M, Seiz O, Herkel J, Schramm C, Flavell RA, Gagliani N, Krebs C, Panzer U, Abdullah Z, Strowig T, Bedke T, and Huber S
- Subjects
- Animals, Cells, Cultured, Dextran Sulfate, Disease Models, Animal, Forkhead Transcription Factors metabolism, Humans, Mice, Mice, Inbred C57BL, Mice, Knockout, Colitis immunology, Inflammation immunology, Inflammatory Bowel Diseases immunology, Liver pathology, T-Lymphocytes, Regulatory immunology, Th17 Cells immunology
- Abstract
Inflammatory bowel disease is associated with extraintestinal diseases such as primary sclerosing cholangitis in the liver. Interestingly, it is known that an imbalance between Foxp3
+ regulatory T cells (Treg) and Th17 cells is involved in inflammatory bowel disease and also in primary sclerosing cholangitis. To explain these associations, one hypothesis is that intestinal inflammation and barrier defects promote liver disease because of the influx of bacteria and inflammatory cells to the liver. However, whether and how this is linked to the Treg and Th17 cell imbalance is unclear. To address this, we used dextran sodium sulfate (DSS) and T cell transfer colitis mouse models. We analyzed the pathological conditions of the intestine and liver on histological, cellular, and molecular levels. We observed bacterial translocation and an influx of inflammatory cells, in particular Th17 cells, to the liver during colitis. In the DSS colitis model, in which Treg were concomitantly increased in the liver, we did not observe an overt pathological condition of the liver. In contrast, the T cell-mediated colitis model, in which Treg are not abundant, was associated with marked liver inflammation and a pathological condition. Of note, upon depletion of Treg in DEREG mice, DSS colitis promotes accumulation of Th17 cells and a pathological condition of the liver. Finally, we studied immune cell migration using KAEDE mice and found that some of these cells had migrated directly from the inflamed intestine into the liver. Overall, these data indicate that colitis can promote a pathological condition of the liver and highlight an important role of Treg in controlling colitis-associated liver inflammation., (Copyright © 2018 by The American Association of Immunologists, Inc.)- Published
- 2018
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7. A Protective Function of IL-22BP in Ischemia Reperfusion and Acetaminophen-Induced Liver Injury.
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Kleinschmidt D, Giannou AD, McGee HM, Kempski J, Steglich B, Huber FJ, Ernst TM, Shiri AM, Wegscheid C, Tasika E, Hübener P, Huber P, Bedke T, Steffens N, Agalioti T, Fuchs T, Noll J, Lotter H, Tiegs G, Lohse AW, Axelrod JH, Galun E, Flavell RA, Gagliani N, and Huber S
- Subjects
- Animals, Cell Movement, Cells, Cultured, Chemical and Drug Induced Liver Injury prevention & control, Chemokine CXCL10 antagonists & inhibitors, Chemokine CXCL10 physiology, Constriction, Hepatectomy, Hepatocytes metabolism, Interleukins deficiency, Interleukins metabolism, Ischemia physiopathology, Liver physiology, Liver Failure, Acute etiology, Liver Failure, Acute prevention & control, Mice, Mice, Inbred C57BL, Mice, Knockout, Monocytes physiology, Receptors, Interleukin deficiency, Receptors, Interleukin genetics, Regeneration, Reperfusion Injury prevention & control, Interleukin-22, Acetaminophen toxicity, Chemical and Drug Induced Liver Injury physiopathology, Liver blood supply, Receptors, Interleukin physiology, Reperfusion Injury physiopathology
- Abstract
Acute liver injury can be secondary to a variety of causes, including infections, intoxication, and ischemia. All of these insults induce hepatocyte death and subsequent inflammation, which can make acute liver injury a life-threatening event. IL-22 is a dual natured cytokine which has context-dependent protective and pathogenic properties during tissue damage. Accordingly, IL-22 was shown to promote liver regeneration upon acute liver damage. However, other studies suggest pathogenic properties of IL-22 during chronic liver injury. IL-22 binding protein (IL-22BP, IL-22Ra2) is a soluble inhibitor of IL-22 that regulates IL-22 activity. However, the significance of endogenous IL-22BP in acute liver injury is unknown. We hypothesized that IL-22BP may play a role in acute liver injury. To test this hypothesis, we used Il22bp -deficient mice and murine models of acute liver damage induced by ischemia reperfusion and N -acetyl- p -aminophenol (acetaminophen) administration. We found that Il22bp -deficient mice were more susceptible to acute liver damage in both models. We used Il22 × Il22bp double-deficient mice to show that this effect is indeed due to uncontrolled IL-22 activity. We could demonstrate mechanistically increased expression of Cxcl10 by hepatocytes, and consequently increased infiltration of inflammatory CD11b
+ Ly6C+ monocytes into the liver in Il22bp -deficient mice upon liver damage. Accordingly, neutralization of CXCL10 reversed the increased disease susceptibility of Il22bp -deficient mice. In conclusion, our data indicate that IL-22BP plays a protective role in acute liver damage, via controlling IL-22-induced Cxcl10 expression., (Copyright © 2017 by The American Association of Immunologists, Inc.)- Published
- 2017
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8. Supercontinuum high-speed cavity-enhanced absorption spectroscopy for sensitive multispecies detection.
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Werblinski T, Lämmlein B, Huber FJ, Zigan L, and Will S
- Abstract
Cavity-enhanced absorption spectroscopy is promising for many applications requiring a very high concentration sensitivity but often accompanied by low temporal resolution. In this Letter, we demonstrate a broadband cavity-enhanced absorption spectrometer capable of detection rates of up to 50 kHz, based on a spatially coherent supercontinuum (SC) light source and an in-house-built, high-speed near-infrared spectrograph. The SC spectrometer allows for the simultaneous quantitative detection of CO
2 , C2 H2 , and H2 O within a spectral range from 1420 to 1570 nm. Using cavity mirrors with a specified reflectivity of R=98.0±0.3% a minimal spectrally averaged absorption coefficient of αmin =1·10-5 cm-1 can be detected at a repetition rate of 50 kHz.- Published
- 2016
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9. A mobile system for a comprehensive online-characterization of nanoparticle aggregates based on wide-angle light scattering and laser-induced incandescence.
- Author
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Huber FJ, Altenhoff M, and Will S
- Abstract
A mobile demonstrator for the comprehensive online-characterization of gas-borne nanoparticle aggregates is presented. Two optical measurement techniques are combined, both utilizing a pulsed Nd:YAG laser as light source. Aggregate size and fractal dimension are measured by Wide-Angle Light Scattering (WALS). An ellipsoidal mirror images elastically scattered light from scattering angles between 10° and 165° onto a CCD-camera chip resulting in an almost complete scattering diagram with high angular resolution. Primary particle size and volume fraction are measured by time-resolved Laser-Induced Incandescence (TiRe-LII). Here, particles are heated up to about 3000 K by the short laser pulse, the enhanced thermal radiation signal is detected with gated photomultiplier tubes. Analysis of the signal decay time and maximum LII-signal allows for the determination of primary particle diameter and volume fraction. The performance of the system is demonstrated by combined measurements on soot nanoparticle aggregates from a soot aerosol generator. Particle and aggregate sizes are varied by using different equivalence ratios of the combustion in the generator. Soot volume fraction can be adjusted by different levels of dilution with air. Online-measurements were carried out demonstrating the favorable performance of the system and the potential for industrial applications such as process control and product development. The particle properties obtained are confirmed through transmission electron microscopy analysis on representative samples.
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- 2016
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10. IL-22BP is regulated by the inflammasome and modulates tumorigenesis in the intestine.
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Huber S, Gagliani N, Zenewicz LA, Huber FJ, Bosurgi L, Hu B, Hedl M, Zhang W, O'Connor W Jr, Murphy AJ, Valenzuela DM, Yancopoulos GD, Booth CJ, Cho JH, Ouyang W, Abraham C, and Flavell RA
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- Animals, Colitis complications, Colitis metabolism, Colitis pathology, Colon metabolism, Colon pathology, Colonic Neoplasms complications, Colonic Neoplasms metabolism, Colonic Neoplasms pathology, Disease Models, Animal, Down-Regulation, Epithelial Cells metabolism, Epithelial Cells pathology, Genes, APC, Interleukin-18 metabolism, Interleukins deficiency, Interleukins genetics, Interleukins metabolism, Mice, Mice, Knockout, Receptors, Interleukin deficiency, Receptors, Interleukin genetics, Time Factors, Weight Loss, Interleukin-22, Cell Transformation, Neoplastic, Inflammasomes metabolism, Intestinal Mucosa metabolism, Intestines pathology, Receptors, Interleukin metabolism
- Abstract
Chronic mucosal inflammation and tissue damage predisposes patients to the development of colorectal cancer. This association could be explained by the hypothesis that the same factors and pathways important for wound healing also promote tumorigenesis. A sensor of tissue damage should induce these factors to promote tissue repair and regulate their action to prevent development of cancer. Interleukin 22 (IL-22), a cytokine of the IL-10 superfamily, has an important role in colonic epithelial cell repair, and its levels are increased in the blood and intestine of inflammatory bowel disease patients. This cytokine can be neutralized by the soluble IL-22 receptor, known as the IL-22 binding protein (IL-22BP, also known as IL22RA2); however, the significance of endogenous IL-22BP in vivo and the pathways that regulate this receptor are unknown. Here we describe that IL-22BP has a crucial role in controlling tumorigenesis and epithelial cell proliferation in the colon. IL-22BP is highly expressed by dendritic cells in the colon in steady-state conditions. Sensing of intestinal tissue damage via the NLRP3 or NLRP6 inflammasomes led to an IL-18-dependent downregulation of IL-22BP, thereby increasing the ratio of IL-22/IL-22BP. IL-22, which is induced during intestinal tissue damage, exerted protective properties during the peak of damage, but promoted tumour development if uncontrolled during the recovery phase. Thus, the IL-22-IL-22BP axis critically regulates intestinal tissue repair and tumorigenesis in the colon.
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- 2012
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11. Th17 cells express interleukin-10 receptor and are controlled by Foxp3⁻ and Foxp3+ regulatory CD4+ T cells in an interleukin-10-dependent manner.
- Author
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Huber S, Gagliani N, Esplugues E, O'Connor W Jr, Huber FJ, Chaudhry A, Kamanaka M, Kobayashi Y, Booth CJ, Rudensky AY, Roncarolo MG, Battaglia M, and Flavell RA
- Subjects
- Animals, CD4-Positive T-Lymphocytes cytology, CD4-Positive T-Lymphocytes metabolism, Cell Proliferation, Colitis immunology, Colitis pathology, Disease Progression, Forkhead Transcription Factors immunology, Interferon-gamma immunology, Interleukin-10 metabolism, Interleukin-10 Receptor alpha Subunit metabolism, Mice, Mice, Inbred C57BL, Peptide Fragments immunology, Signal Transduction, Th17 Cells cytology, Th17 Cells metabolism, CD4-Positive T-Lymphocytes immunology, Interleukin-10 immunology, Interleukin-10 Receptor alpha Subunit immunology, Th17 Cells immunology
- Abstract
T helper 17 (Th17) cells are important for host defense against extracellular microorganisms. However, they are also implicated in autoimmune and chronic inflammatory diseases, and as such need to be tightly regulated. The mechanisms that directly control committed pathogenic Th17 cells in vivo remain unclear. We showed here that IL-17A-producing CD4+ T cells expressed interleukin-10 receptor α (IL-10Rα) in vivo. Importantly, T cell-specific blockade of IL-10 signaling led to a selective increase of IL-17A+IFN-γ⁻ (Th17) and IL-17A+IFN-γ+ (Th17+Th1) CD4+ T cells during intestinal inflammation in the small intestine. CD4+Foxp3⁻ IL-10-producing (Tr1) cells and CD4+Foxp3+ regulatory (Treg) cells were able to control Th17 and Th17+Th1 cells in an IL-10-dependent manner in vivo. Lastly, IL-10 treatment of mice with established colitis decreased Th17 and Th17+Th1 cell frequencies via direct signaling in T cells. Thus, IL-10 signaling directly suppresses Th17 and Th17+Th1 cells., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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12. The comparative efficacy of benzoyl peroxide 5%/erythromycin 3% gel and erythromycin 4%/zinc 1.2% solution in the treatment of acne vulgaris.
- Author
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Chu A, Huber FJ, and Plott RT
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- Acne Vulgaris pathology, Adolescent, Adult, Drug Combinations, Humans, Patient Satisfaction, Single-Blind Method, Zinc therapeutic use, Acne Vulgaris drug therapy, Anti-Bacterial Agents therapeutic use, Benzoyl Peroxide therapeutic use, Erythromycin therapeutic use, Keratolytic Agents therapeutic use
- Abstract
This randomized 10-week study compared the efficacy of benzoyl peroxide 5%/erythromycin 3% gel with erythromycin 4%/zinc 1.2% solution in 72 acne vulgaris patients. Physician global evaluations were significantly more improved (P < or = 0.05) in the benzoyl peroxide 5%/erythromycin 3% gel treatment group compared to erythromycin 4%/zinc 1.2% solution at week 2 and at each subsequent biweekly clinical visit. Inflammatory lesions (papules/pustules) were significantly more reduced (P < or = 0.005) in the benzoyl peroxide 5%/erythromycin 3% gel treatment group than the erythromycin 4%/zinc 1.2% solution at weeks 4 and 10. Comedones were significantly more reduced (P < or = 0.001) in the benzoyl peroxide 5%/erythromycin 3% gel treatment group than in the erythromycin 4%/zinc 1.2% solution group at weeks 8 and 10. Patient efficacy evaluations significantly (P < or = 0.001) favoured benzoyl peroxide 5%/erythromycin 3% gel to erythromycin 4%/zinc 1.2% solution.
- Published
- 1997
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