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Cuproptosis is a copper-induced form of mitochondrial cell death which is engaged in the proliferation and migration of a variety of tumors. Nevertheless, the role of cuproptosis in tumor microenvironment (TME) remodeling and antitumor therapy is still poorly understood. We characterized two diverse cuproptosis-associated molecular isoforms in CRC which exhibit distinct prognostic and TME characteristics. Subsequently, we constructed a cuproptosis-associated prognostic model containing five genes and divided the patients into a high CPS-score group and a low CPS-score group. Univariate and multivariate Cox analyses showed that the CPS score could be used as an independent prognostic factor. The nomogram, and its consequent calibration curves, indicated that this prognostic signature had good predictive power for CRC. The analysis of single-cell sequencing data showed the significant expression of HES4 and SPHK1 in various immune and stromal (including fibroblasts) cells. Further studies showed that tumor mutational burden (TMB), high microsatellite instability (MSI-H) ratio, immune checkpoint blockade (ICB), and human leukocyte antigen (HLA) gene expression all positively correlated with the CPS score, predicting a better reaction to immunotherapy in high CPS-core patients. The CPS score constructed from cuproptosis subtypes can be used as a predictive tool to evaluate the prognosis of CRC patients and their response to immunotherapy.

Thirty-three novel paeonol etherized aryl urea derivatives (PEUs) were synthesized via a bromination-Williamson Ether Synthesis-deprotection-nucleophilic addition reaction sequence. The structures of PEUs were characterized by LC-MS, HRMS

The treatment of shale gas produced water (SGPW) for beneficial reuse is currently the most dominant and economical option. Membrane filtration is one preferred method to deal with SGPW, but membrane fouling is an unavoidable problem. In this study, two types of ultrafiltration (UF) membranes and one type of microfiltration (MF) membrane were investigated to treat SGPW from Sichuan basin. Results showed that increased total dissolved solid (31-40 g/L) and UV

In this study, inclusion complex of cis-jasmone in β-CD (β-CD-CJ) was synthesized to improve cis-jasmone stability. The structure and thermal kinetics of the inclusion complex was investigated by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG) and differential scanning calorimetry (DSC). DSC studies showed that the stability of cis-jasmone after β-cyclodextrin encapsulation was improved. The dissociation kinetics of β-CD-CJ at different heating rates was studied by TG, and the activation energy E of β-CD-CJ thermal decomposition kinetic parameters was defined by Flynn–Wall–Ozawa method. The results showed that the average activation energy E was 121.16 kJ mol−1.

e21503 Background: Treatment options are limited for patients with advanced melanoma and refractory melenoma, especially for acral and mucosal melanoma. Previous data showed that the objective response rate (ORR) of second-line PD1 monotherapy for acral and mucosal melanoma were 14-15.8% and 0-13.3%, respectively. We evaluate the safety and efficacy of recombinant human adenovirus 5 injection (oncolytic virus) plus toripalimab (PD-1 antibody) for patients who have progressed on checkpoint inhibitors, chemotherapy and anti-angiogenesis. Preliminary analysis of efficacy- related factors. Methods: Patients received the recombinant human adenovirus 5 injection (2ml) local lesions plus toripalimab 240mg systemic therapy every 2 weeks until progression or intolerable toxicity. The primary objective was safety and ORR. Secondary objective included progression-free survival (PFS) and disease control rate (DCR). Efficacy assessments were performed every 2 months according to RECIST v1.1 criteria. Blood samples were collected from patients prospectively. Serum interleukin (IL)-2, IL-4, IL-6, IL-8, IL-10, Tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) were measured at baseline and after two cycles of treatment via flow cytometry. The peripheral blood neutrophils and lymphocytes were collected simultaneously. Results: Ten cases of patients were enrolled from November 2020 to January 2022. Baseline character:primary site (1 cutaneous melanoma; 5 acral melanoma; 4 mucosal melanoma), prior therapy (anti-PD1 100%; anti-angiogenesis 60%; chemotherapy 60%; second line and above 40%), 50% liver/bone lesions. All patients evaluated received at least 2 cycles of the combination regimen. The treatment-related adverse event was fever, accounting for 80%, and there were no other adverse events. The ORR was 20% (2PR) and the DCR was 70% (2PR, 5SD, 3PD). The 2-month PFS was 70%, and the median PFS has not yet been reached. Disease progression in 3 patients including liver and bone lesion. Higher baseline lymphocyte levels and lower neutrophil-to-lymphocyte ratio (NLR) values were found in the respongders compared with non-responders. The serum IL-6, IL-8, IL-10, and TNF-α levels were significantly increased in the responders after 2 cycles of treatment. Conclusions: The preliminary data showed that the combination of recombinant human adenovirus 5 injection plus toripalimab demonstrated acceptable toxicity and promising antitumor efficacy in patients with advanced and refractory melanoma, and promoted favorable changes in serum IL-6, IL-8, IL-10 and TNF-α levels in clinical responders. High baseline lymphocyte and low NLR values may be predictors of treatment response. Long-term survival data remain to continue with follow-up. It is worth to further validate the efficacy in a randomized prospective trial. Clinical trial information: 2000033959.

Two series of fluorine-containing fluorenyl anthracenes linked by a rigid sp3-hybridized carbon atom have been synthesized and characterized. The effect of a fluorine auxochrome at different positions in fluorene moiety on bulk properties, such as photophysical, electrochemical properties and thermal stabilities were investigated. The non-doped organic light-emitting diodes (OLEDs) utilizing C9-methylated fluorinated fluorenyl anthracene 4b as the emitter exhibits deep-blue emissions CIE x, y (0.159, 0.106) with efficiency of 1.31 cd/A and a maximum brightness of 2040 cd/m2 at 14 V. It was found that the introduction of fluorine auxochrome to the C2 position of fluorene could afford the best performances of OLEDs among the fluorinated materials.

In this study, inclusion complex of

The Ag(I)-catalyzed decarboxylative homocoupling from the difluoroacetate has been developed to the synthesis of symmetric CF2–CF2 containing dimers. This radical dimerization overpasses the prefunctionalization of the substrate and provides a direct and efficient method for construction of tetrafluoroethylene bridge-linked homodimers.

Fe-C micro-electrolysis system has been widely used in filters, or as an advanced treatment process in some water treatment plants to treat various wastewater. In this study, Fe-C micro-electrolysis process was enhanced by an economical and environmentally friendly method, applied magnetic field. Batch kinetic experiments and scanning electron micrographs demonstrated a more effective micro-electrolysis and more severely corroded on the surface of Fe-C after applying a magnetic field at pH 3.0. An applied magnetic field reduced the charge-transfer resistance and increased the current density in micro-electrolysis system and Fe-C became more prone to electrochemical corrosion. Corrosion products were proved to be Fe2+, Fe3O4, and C-O, moreover, the formation of them were also increased in the presence of a magnetic field. Base on that, some influential factors like magnetic field flux intensity, Fe-C particle size, pH, Fe-C dosage and its reusability were investigated in this paper. Since Fe2+ release was accelerated in micro-electrolysis system by an applied magnetic field, combination of various advanced oxidation processes were designed to explore the application effectiveness of the system. The degradation rate of target contaminant was significantly improved in the presence of a magnetic field, suggesting it could be a reliable method for wastewater treatment.

In this paper, we consider a type of nth order functional difference systems depending on a parameter λ. By using the fixed point index theory, we prove that there exists a positive number \({\widetilde{\lambda}}\) separating \({(0,+ \infty) \backslash \widetilde{\lambda}}\) into two disjoint subintervals Λ1 and Λ2 such that the system has zero, at least one or at least two positive periodic solutions according to \({\lambda \in \Lambda_1, \lambda = \widetilde{\lambda}}\), or \({\lambda \in \Lambda_2}\). This work improves and extends some recent results in the literature for the first order systems. In addition, we obtain conditions for a general system to have nontrivial periodic solutions. The results are illustrated with an example.