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Metronidazole (MNZ) accumulation inhibits municipal wastewater treatment bio-systems, and an effective solution to augment anaerobic activated sludge (AAS) is required. This research discovered that Aspergillus tabacinus LZ-M could degrade 77.39% of MNZ at 5 mg/L. MNZ was metabolized into urea, and the enzymes involved in its degradation were aminotransferase, methyltransferase, monooxygenase, and CN cleavage hydrolase. The strain was immobilized in polyurethane foam and used in AAS for the treatment of MNZ-containing municipal wastewater. The results showed that, using immobilized LZ-M, MNZ was completely removed, and the degradation efficiency of wastewater's chemical oxygen demand (COD) was increased from 11.7% to 83.31%. The extracellular polymer and ROS levels indicated that MNZ's toxicity on AAS was reduced. Furthermore, bioaugmentation stabilized its microbial community, and decreased MNZ resistance genes. These observations confirm that the immobilized fungi are effective in protecting AAS against antibiotic contamination in the treatment process of municipal wastewater.

Probiotics have demonstrated the potential to ameliorate hyperuricemia in animals, but their effectiveness and mechanism of action in humans has been understudied. A randomized, double-blinded, controlled research was conducted with 120 volunteers, who consume either a probiotic yogurt containing a UA-degrading strain

Copper pollution poses a serious threat to the aquatic environment; however, in situ analytical methods for copper monitoring are still scarce. In the current study, Escherichia coli Rosetta was genetically modified to express OprF and ribB with promoter Pt7 and PcusC, respectively, which could synthesize porin and senses Cu2+ to produce riboflavin. The cell membrane permeability of this engineered strain was increased and its riboflavin production (1.45–3.56 μM) was positively correlated to Cu2+ (0–0.5 mM). The biosynthetic strain was then employed in microbial fuel cell (MFC) based biosensor. Under optimal operating parameters of pH 7.1 and 37°C, the maximum voltage (248, 295, 333, 352, and 407 mV) of the constructed MFC biosensor showed a linear correlation with Cu2+ concentration (0.1, 0.2, 0.3, 0.4, 0.5 mM, respectively; R2 = 0.977). The continuous mode testing demonstrated that the MFC biosensor specifically senses Cu2+ with calculated detection limit of 28 μM, which conforms to the common Cu2+ safety standard (32 μM). The results obtained with the developed biosensor system were consistent with the existing analytical methods such as colorimetry, flame atomic absorption spectrometry, and inductively coupled plasma optical emission spectrometry. In conclusion, this MFC-based biosensor overcomes the signal conversion and transmission problems of conventional approaches, providing a fast and economic analytical alternative for in situ monitoring of Cu2+ in water. © 2020 Wiley Periodicals LLC

Biochars produced by pyrolysis at high temperatures under oxygen limited conditions can contain both well-known contaminants (polycyclic aromatic hydrocarbons (PAHs), potentially toxic elements (PTEs), dioxins, volatile organic compounds (VOCs)) and emerging contaminants (e.g., persistent free radicals, metal cyanide). Their potential to induce phytotoxicity, cytotoxicity, and neurotoxicity highlight the need to establish effective strategies to control and eliminate contaminants for sustainable biochar use. Although some articles have reviewed the ecotoxic potential of biochar in relation to some of these contaminants, strategies to mitigate the whole suite of contaminants potentially present in biochar have not been systematically reviewed so far. This review discusses (i) the formation mechanism of such contaminants and (ii) evaluates their potential risk to ecosystems, a prerequisite (iii) to understand and explore effective control strategies for producing biochars with minimum contamination. A pyrolysis unit design where pyrolysis liquids and solids are fully separated following the pyrolysis zone and optimization of pyrolysis parameters ensures organic contaminants in the pyrolysis liquids do not condense onto the biochar. Post-production treatments, such as thermal treatment or natural and artificial ageing help organic contaminant removal and breakdown. Co-pyrolysis of metal-rich feedstock with metal-free biomass is the only way of reducing total PTE levels, though available PTE levels are low in biochars and decrease further with pyrolysis temperature. More specific strategies for reducing the concentration of individual contaminants are discussed in the review. With our proposed recommendations, biochars with little risk for the environment can be produced.

Existing studies reported higher altitudes reduce the COVID-19 infection rate in the United States, Colombia, and Peru. However, the underlying reasons for this phenomenon remain unclear. In this study, regression analysis and mediating effect model were used in a combination to explore the altitudes relation with the pattern of transmission under their correlation factors. The preliminary linear regression analysis indicated a negative correlation between altitudes and COVID-19 infection in China. In contrast to environmental factors from low-altitude regions (1500 m), high-altitude regions (1500 m) exhibited lower PM2.5, average temperature (AT), and mobility, accompanied by high SO

Specific roles of gut microbes in COVID-19 progression are critical. However, the circumstantial mechanism remains elusive. In this study, shotgun metagenomic or metatranscriptomic sequencing was performed on fecal samples collected from 13 COVID-19 patients and controls. We analyzed the structure of gut microbiota, identified the characteristic bacteria, and selected biomarkers. Further, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations were employed to correlate the taxon alterations and corresponding functions. The gut microbiota of COVID-19 patients was characterized by the enrichment of opportunistic pathogens and depletion of commensals. The abundance of

Adsorption techniques offer unique advantages owing to the use of synthetic (e.g., nanosized metal oxides and polymer-functionalized nanocomposites) and natural (e.g., clay and biochar) materials for pollutant removal. Although the most widely used adsorbent is activated carbon, extensive studies have highlighted the promising potential of modified clay minerals and biochar for removing heavy metal and organic pollutants from industrial, drinking, and eutrophic wastewater, due to their low cost and easy accessibility. However, clay modification using acids, calcination, polymers, or surfactants exhibits relatively low absorption/regeneration ability towards antibiotics, aromatics, and various dyes. The coexistence of numerous contaminants in industrial wastewater inhibited the performance of adsorbents, which accelerated the development of novel modified clay composites such as clay-biochar, organo-bentonite/sodium alginate beads, and enhanced biochar. This review summarizes recent studies and absorption mechanisms concerning clay composites based on various modification methods and component materials. The comparison of clay composites used for the removal of organic and inorganic contaminants provides valuable insight into real wastewater treatment. Knowledge gaps, uncertainties, and future challenges involved in the fabrication and regeneration of modified clay composites are also identified.

Yak dung is used as fuel in Tibetan homes; however, this use is hazardous to health. An alternative use of the dung that would be profitable and offset the loss as a fuel would be very beneficial. Sweet sorghum silage with yak dung biochar as an additive was compared with a control silage with no additives and three silages with different commercial additives, namely Lactobacillus buchneri, Lactobacillus plantarum and Acremonium cellulase. Biochar-treated silage had a significantly greater concentration of water-soluble carbohydrates than the other silages (76 vs 12.4-45.8 g/kg DM) and a greater crude protein content (75.5 vs 61.4 g/kg DM), lactic acid concentration (40.7 vs 27.7 g/kg DM) and gross energy yield (17.8 vs 17.4 MJ/kg) than the control silage. Biochar-treated and control silages did not differ in in vitro digestibility and in total gas (507 vs 511 L/kg DM) and methane production (57.9 vs 57.1 L/kg DM). Biochar inhibited degradation of protein and water-soluble carbohydrates and enhanced lactic acid production, which improved storability of feed. It was concluded that yak dung biochar is an efficient, cost-effective ensiling additive. The profit could offset the loss of dung as fuel and improve the health of Tibetan people.

Cu(II)-enhanced microbial Cr(VI) reduction is common in the environment, yet its mechanism is unknown. The specific activity of chromate reductase, NfoR, from Staphylococcus aureus sp. LZ-01 was augmented 1.5-fold by Cu(II). Isothermal titration calorimetry and spectral data show that Cu(II) binds to NfoR nonspecifically. Further, Cu(II) stimulates the nitrobenzene reduction of NfoR, indicating that Cu(II) promotes electron transfer. The crystal structure of NfoR in complex with CuSO4 (1.46 A) was determined. The overall structure of NfoR-Cu(II) complex is a dimer that covalently binds with FMN and Cu(II)-binding pocket is located at the interface of the NfoR dimer. Structural superposition revealed that NfoR resembles the structure of class II chromate reductase. Site-directed mutagenesis revealed that Leu46 and Phe123 were involved in NADH binding, whereas Trp70 and Ser45 were the key residues for nitrobenzene binding. Furthermore, His100 and Asp171 were preferential affinity sites for Cu(II) and that Cys163 is an active site for FMN binding. Attenuation reductase activity in C163S can be partially restored to 54% wild type by increasing Cu(II) concentration. Partial restoration indicates dual-channel electron transfer of NfoR via Cu(II) and FMN. We propose a catalytic mechanism for Cu(II)-enhanced NfoR activity in which Cu(I) is formed transiently. Together, the current results provide an insight on Cu (II)-induced enhancement and benefit of Cr(VI) bioremediation.

Heavy metal contamination in food endangers human health. Probiotics can protect animals and human against heavy metals, but the detoxification mechanism has not been fully clarified. Here, mice were supplemented with Pediococcus acidilactici strain BT36 isolated from Tibetan plateau yogurt, with strong antioxidant activity but no chromate reduction ability for 20 days to ensure gut colonization. Strain BT36 decreased chromate accumulation, reduced oxidative stress, and attenuated histological damage in the liver of mice. 16S rRNA and metatranscriptome sequencing analysis of fecal microbiota showed that BT36 reversed Cr(VI)-induced changes in gut microbial composition and metabolic activity. Specifically, BT36 recovered the expressions of 788 genes, including 34 inherent Cr remediation-relevant genes. Functional analysis of 10 unannotated genes regulated by BT36 suggested the existence of a new Cr(VI)-reduction gene in the gut microbiota. Thus, BT36 can modulate the gut microbiota in response to Cr(VI) induced oxidative stress and protect against Cr toxicity., Feng et al. show that colonization of mice with Pediococcus acidilactici strain BT36 reduces chromate accumulation in the liver, which is associated with changes in expression of a Cr(VI)-resistance gene in the gut microbiota. This study suggests that BT36 regulates the gut microbiota in response to chrome exposure, protecting mice against chromate toxicity.

In the past forty years, China’s struggle with the environmental pollution is accompanied with rapid economic development driven by the reform and opening up policy. The Chinese government takes a series of measures to address environmental deterioration. However, economic growth for priority and poor policy execution impeded the environmental governance effect. This chapter summarized the development phase of environmental managements in China and provided a comparative analysis of environmental policies among different countries. By contrast with the environmental policies of northern Europe and the USA, we provide a critical information on the successful and failed case from environmental policy implementation. Furthermore, we further evaluate the potential challenges of environmental protection, including monitoring, risk assessment, and policy implementation. Thus, environmental management policies, coupled with gut remediation provide an insight into environmental control and human health.

Overexpression of the enzyme phosphatase (PhoN/PhoK) in the radiation-resistant bacterium Deinococcus radiodurans could be an efficient strategy for uranium remediation. However, the presence of other metals in nuclear wastes often interferes with uranium bioprecipitation. In our study, the uranium-precipitating ability of the PhoN-expressing D. radiodurans strain (Deino-phoN) significantly decreased by 45.4% in 13 h in the presence of chromium (VI); however, it was partially recovered after supplementation with chromium (III). Therefore, the reduction of chromium (VI) to chromium (III) was obtained by the co-expression of the YieF protein and PhoN in D. radiodurans (Deino-phoN-yieF). As a result, an increase in the chromium (VI) reduction (25.1%) rate was observed in 24 h. Furthermore, uranium precipitation also increased by 28.0%. For the decontamination of groundwater, we immobilized Deino-phoN-yieF cells using Polyvinyl alcohol (PVA)-sodium alginate (SA) beads, followed by incubation in a bioreactor. Approximately 99% of chromium (VI) and uranium (VI) was removed after 4 continuous cycles operated for a period of over 20 days at room temperature (25 °C). Therefore, Deino-phoN-yieF could be used as a potential biological agent for mixed radioactive nuclear waste remediation.

Eutrophication is a global concern that has severely deteriorated aquatic ecosystems. This phenomenon has impacted the freshwater bodies (such as lakes and rivers) across the world due to the nutrients (mainly N and P) arising from point and non-point pollution sources. Nutrient enrichment induces cyanobacteria formation, hypoxia, and release of bloom toxins, causing their accumulation in aquatic organisms and humans. Preventing the use of chemicals (such as fertilizers) at the source can avoid nutrient release in the run-off streams, thus controlling non-point pollution. While point source pollutants can be effectively controlled at the sewage treatment plants. Although freshwater eutrophication managements have been widely discussed, a systematic eutrophication characterization of lakes and rivers worldwide, with their effective, economically feasible, eco-friendly, and beneficial bio-treatments is still scarce. Hence, in this review, a complete eutrophication-based characterization of lakes and rivers all over the world is provided. Different cost-effective and efficient bio-remediations to control point and non-point pollution are discussed, additionally with a comparative account for the selection of a suitable microbe and remediation approach. A combined point and non-point pollution management can eradicate eutrophication.

Microbial fuel cell (MFC) is considered to be a sustainable technology for cellulosic waste treatment and energy recovery; however, studies on cellulose-degrading exoelectrogens are scarce. Giant panda gut microbiota may provide potential cellulose-degrading exoelectrogens due to consumption of high-fiber diet. In this study, strain LZ-P1 isolated from giant panda gut simultaneously showed cellulose-degrading and electricity-generating characteristics. 16S rRNA gene sequence revealed 99% homology with Cellulomonas iranensis. Results showed that the strain generated 1.08 U/mL endoglucanase and 180 mV electricity when cellulose was used as the sole carbon source. Genome sequence confirmed that strain LZ-P1 possessed complete cellulose degradation and extracellular electron transfer pathways. Proteomic analysis further confirmed that the enzymes involved in cellulose-degradation and extracellular electron transport were remarkably up-regulated, including endoglucanases, riboflavin biosynthesis protein, and cytochrome C oxidase. When strain LZ-P1 was applied to MFC-mediated paper recycling wastewater treatment, a maximum power density of 44.05 mW/m2 and 8.72% coulombic efficiency were recovered in 72 h. Therefore, strain LZ-P1 would be a potential candidate for cellulosic waste management and energy recovery.

Thermozymes (from thermophiles or hyperthermophiles) offer obvious advantages due to their excellent thermostability, broad pH adaptation, and hydrolysis ability, resulting in diverse industrial applications including food, paper, and textile processing, biofuel production. However, natural thermozymes with low yield and poor adaptability severely hinder their large-scale applications. Extensive studies demonstrated that using genetic modifications such as directed evolution, semi-rational design, and rational design, expression regulations and chemical modifications effectively improved enzyme's yield, thermostability and catalytic efficiency. However, mechanism-based techniques for thermozymes improvements and applications need more attention. In this review, stabilizing mechanisms of thermozymes are summarized for thermozymes improvements, and these improved thermozymes eventually have large-scale industrial applications.

Lignin compound wastes are generated as a result of agricultural and industrial practices. Microorganism-mediated bio-catalytic processes can depolymerize and utilize lignin eco-friendly. Although fungi have been studied since several decades for their ability to depolymerize lignin, strict growth conditions of fungus limit it's industrial application. Compared with fungi, bacteria can tolerate wider pH, temperature, oxygen ranges and are easy to manipulate. Several studies have focused on bacteria involved in the process of lignin depolymerization and utilization. Pseudomonas have been used for paper mill wastewater treatment while Rhodococcus are widely reported to accumulate lipid. In this review, the recent studies on bacterial utilization in paper wastewater treatment, lignin conversion to biofuels, bioplastic, biofertilizers and other value-added chemicals are summarized. As bacteria possess remarkable advantages in industrial production, they may play a promising role in the future commercial lignin utilization.

Microbes can reduce hexavalent chromium Cr (VI) to the less toxic and soluble trivalent Cr (III). Copper stimulates microbial reduction of Cr (VI) by the Bacillus, Ochrobactrum, and Gluconobacter species; however, the mechanism remains unclear. In our study, the rate of Cr (VI) reduction by Staphylococcus aureus LZ-01 was increased by 210 % when supplemented with 60 μM Cu (II). A putative NAD(P)H-flavin oxidoreductase gene (nfoR) was upregulated under Cr (VI) stress. NfoR-knockout mutant displayed impaired reduction of Cr (VI) and Cu (II)-enhanced Cr (VI) reduction by nfoR isogenic mutant was attenuated in the presence of Cu (II). In vitro tests showed an increased V max value of 25.22 μM min−1 mg−1 NfoR in the presence of Cu (II). Together, these results indicate that NfoR is responsible for Cu (II) enhancement. Isothermal titration calorimetry (ITC) assays confirmed the interaction of NfoR with Cu (II) at the dissociation constant of 85.5 μM. Site-directed mutagenesis indicates that His100, His128, and Met165 residues may be important for Cu (II) binding, while Cys163 is necessary for the FMN binding of NfoR. These findings show that Cu (II)-enhanced NfoR belongs to a new branch of Cr (VI) reductases and profoundly influences Cr (VI) reduction.

With the unprecedented deterioration of environmental quality, rapid recognition of toxic compounds is paramount for performing in situ real-time monitoring. Although several analytical techniques based on electrochemistry or biosensors have been developed for the detection of toxic compounds, most of them are time-consuming, inaccurate, or cumbersome for practical applications. More recently, microbial fuel cell (MFC)-based biosensors have drawn increasing interest due to their sustainability and cost-effectiveness, with applications ranging from the monitoring of anaerobic digestion process parameters (VFA) to water quality detection (e.g., COD, BOD). When a MFC runs under correct conditions, the voltage generated is correlated with the amount of a given substrate. Based on this linear relationship, several studies have demonstrated that MFC-based biosensors could detect heavy metals such as copper, chromium, or zinc, as well as organic compounds, including p-nitrophenol (PNP), formaldehyde and levofloxacin. Both bacterial consortia and single strains can be used to develop MFC-based biosensors. Biosensors with single strains show several advantages over systems integrating bacterial consortia, such as selectivity and stability. One of the limitations of such sensors is that the detection range usually exceeds the actual pollution level. Therefore, improving their sensitivity is the most important for widespread application. Nonetheless, MFC-based biosensors represent a promising approach towards single pollutant detection.