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4 results on '"Huanmeng Hao"'

1. Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

Author

Yin Luo, Huanmeng Hao, Zhangjie Wang, Chih Yean Ong, Robert Dutcher, Yongmei Xu, Jian Liu, Lars C Pedersen, and Ding Xu

Subjects
oligomerization, death receptor, internalization, TNF, strand swapping, Medicine, Science, Biology (General), QH301-705.5
Abstract

TRAIL (TNF-related apoptosis-inducing ligand) is a potent inducer of tumor cell apoptosis through TRAIL receptors. While it has been previously pursued as a potential anti-tumor therapy, the enthusiasm subsided due to unsuccessful clinical trials and the fact that many tumors are resistant to TRAIL. In this report, we identified heparan sulfate (HS) as an important regulator of TRAIL-induced apoptosis. TRAIL binds HS with high affinity (KD = 73 nM) and HS induces TRAIL to form higher-order oligomers. The HS-binding site of TRAIL is located at the N-terminus of soluble TRAIL, which includes three basic residues. Binding to cell surface HS plays an essential role in promoting the apoptotic activity of TRAIL in both breast cancer and myeloma cells, and this promoting effect can be blocked by heparin, which is commonly administered to cancer patients. We also quantified HS content in several lines of myeloma cells and found that the cell line showing the most resistance to TRAIL has the least expression of HS, which suggests that HS expression in tumor cells could play a role in regulating sensitivity towards TRAIL. We also discovered that death receptor 5 (DR5), TRAIL, and HS can form a ternary complex and that cell surface HS plays an active role in promoting TRAIL-induced cellular internalization of DR5. Combined, our study suggests that TRAIL-HS interactions could play multiple roles in regulating the apoptotic potency of TRAIL and might be an important point of consideration when designing future TRAIL-based anti-tumor therapy.

Published
2024
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2. Cyclic compression emerged dual effects on the osteogenic and osteoclastic status of LPS-induced inflammatory human periodontal ligament cells according to loading force

Author

Ru Jia, Yingjie Yi, Jie Liu, Dandan Pei, Bo Hu, Huanmeng Hao, Linyue Wu, Zhenzhen Wang, Xiao Luo, and Yi Lu

Subjects
Periodontitis, hPDLCs, LPS, Dynamic loading, Osteogenic differentiation, Dentistry, RK1-715
Abstract

Abstract Background Appropriate mechanical stimulation is essential for bone homeostasis in healthy periodontal tissues. While the osteogenesis and osteoclast differentiation of inflammatory periodontal ligament cells under different dynamic loading has not been yet clear. The aim of this study is to clarify the inflammatory, osteogenic and pro-osteoclastic effects of different cyclic stress loading on the inflammatory human periodontal ligament cells (hPDLCs). Methods hPDLCs were isolated from healthy premolars and cultured in alpha minimum Eagle’s medium (α-MEM). Lipopolysaccharides (LPS) were used to induce the inflammation state of hPDLCs in vitro. Determination of LPS concentration for the model of inflammatory periodontium was based on MTT and genes expression analysis. Then the cyclic stress of 0, 0–50, 0–90 and 0–150 kPa was applied to the inflammatory hPDLCs for 5 days respectively. mRNA and protein levels of osteogenic, osteoclastic and inflammation-related markers were examined after the treatment. Results MTT and RT-PCR results showed that 10 μg/ml LPS up-regulated TNF-α, IL-1β, IL-6, IL-8 and MCP-1 mRNA levels (P 0.05). The excessive loading of stress (150 kPa) with or without LPS strongly increased the expression of inflammatory-related markers TNF-α, IL-1β, IL-6, IL-8, MCP-1 (P

Published
2020
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3. Comparative evaluation of the morphological accuracy of dental crowns fabricated by different technologies

Author

Yi Lu, Fang Wang, Huanmeng Hao, and Qingqing Tang

Subjects
Orthodontics, Typodont, Dental Impression Technique, Materials science, Crowns, Post hoc, X-Ray Microtomography, 030206 dentistry, Ceramic crown, Dental Marginal Adaptation, Dental Porcelain, Mandibular first molar, Comparative evaluation, Root mean square, Dental crowns, 03 medical and health sciences, Imaging, Three-Dimensional, 0302 clinical medicine, Least significant difference, Dental Prosthesis Design, Computer-Aided Design, Oral Surgery
Abstract

Information on the morphological accuracy of crowns produced by different technologies is limited.The purpose of this in vitro study was to compare the morphology and contacts of crowns fabricated with intraoral systems, extraoral systems, and conventional method.A typodont mandibular first molar (Nissin Dental Product) received a complete ceramic crown preparation and a reference crown. Microcomputed tomography (μCT) was used to obtain the virtual data (REF) of the reference crown. Three groups of replicated crowns were made: intraoral scanning system (TRIOS), extraoral scanning system (D700), and the conventional method (CONV) (n=8). The groups TRIOS and D700 were designed by the correlation method. All crowns were scanned with μCT to obtain 3D data. The data were superimposed on each other or the REF in an inspection software to evaluate precision and trueness. The contact penetration area of the occlusal surfaces of the crowns was calculated. An independent sample t test and 1-way ANOVA with the post hoc least significant difference (LSD) test were used to compare the data (α=.05).The crowns fabricated with the extraoral scanners showed significantly lower root mean square (RMS) values for trueness (F=1456.90, df=2, P.001) and precision (F=188.88, df=2, P.001) than the others. The penetration contact area ratio and the differences in the CONV group were both significantly higher than those of the other groups.The average discrepancies of the crown morphology fabricated from the extraoral scanning were significantly lower than those from others. The conventional method restored the occlusal contact with significantly less accuracy than the other groups.

Published
2021
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4. Cyclic compression emerged dual effects on the bone homeostasis of LPS-induced inflammatory human periodontal ligament cells according to loading force

Author

Ru Jia, Yingjie Yi, Jie Liu, Dandan Pei, Bo Hu, Huanmeng Hao, Linyue Wu, Zhenzhen Wang, Xiao Luo, and Yi Lu

Abstract

Background: Appropriate mechanical stimulation is essential for bone homeostasis in healthy periodontal tissues. While the bone homeostasis of inflammatory periodontal tissues under different dynamic loading has not been yet clear. The aim of this study is to clarify the inflammatory, osteogenic and pro-osteoclastic effects of different cyclic stress loading on the inflammatory human periodontal ligament cells (hPDLCs). Methods: hPDLCs were isolated from healthy premolars and cultured in alpha minimum Eagle’s medium (α-MEM). Lipopolysaccharides (LPS) were used to induce the inflammation state of hPDLCs in vitro. Determination of LPS concentration for the model of inflammatory periodontium was based on MTT and genes expression analysis. Then the cyclic stress of 0, 0-50, 0-90 and 0-150 kPa was applied to the inflammatory hPDLCs for 5 days respectively. mRNA and protein levels of osteogenic, osteoclastic and inflammation-related markers were examined after the treatment. Results: MTT and RT-PCR results showed that 10 μg/ml LPS up-regulated TNF-α, IL-1β, IL-6, IL-8 and MCP-1 mRNA levels (P0.05). The excessive loading of stress (150 kPa) with or without LPS strongly increased the expression of inflammatory-related markers TNF-α, IL-1β, IL-6, IL-8, MCP-1 (P

Published
2019
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