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ObjectiveTo investigate the contamination status of Burkholderia gladioli in commercially available mushroom products in Chengdu， analyze its pathogenic characteristics， and provide support for food safety risk monitoring.MethodsAccording to GB 4789.29—2020， the identification of suspected colony by microbial mass spectrometry was added. One hundred and twenty-one mushroom foods were detected， and the whole genome of the isolated strains was sequenced to analyze their genetic characteristics and the carrying status of bon genes related to the biosynthesis of Bongkrekic acid.ResultsThe positive rate of Burkholderia gladioli in 121 samples was 50.41% （61/121）， with a positive rate of 67.14% （47/70） for Tremella； 10 strains of Burkholderia gladioli isolated from 4 food samples carried bon genes clusters； There was a dominant clone group that could mainly contaminated Tremella， without carrying the bon gene clusters.ConclusionTremella could be easily contaminated by Burkholderia gladioli， so it is necessary to strengthen the risk monitoring for the detection of this pathogen in key foods， especially for the strains carrying the bon gene clusters.

Warfarin is an anticoagulant commonly used as an oral drug in preventing and treating thromboembolic diseases. The international normalized ratio (INR) is a clinical monitoring anticoagulation intensity index that adjusts the dose based on important references. In particular, INR value must be strictly monitored when warfarin is used for anticoagulation therapy in infected patients. Herein, we report a 54-year-old female patient diagnosed with pulmonary embolism and venous thrombosis of the lower limbs. After the warfarin administration, the INR was always substandard. The patient did not take other warfarin-interacting drugs or foods during the hospital stay. Metagenome next-generation sequencing suggested a bloodstream infection caused by Escherichia coli, which was further confirmed by blood culture. After meropenem administration for anti-infective treatment, the INR value rose rapidly to a standard level. Considering the lack of relevant reports, this case is the first report of potential interaction between E. coli and warfarin. Further, in patients with thromboembolic diseases complicated by infection, antibiotics should be chosen reasonably with close monitoring of the INR to avoid the interaction of warfarin and antibiotics and to ensure the effectiveness and safety of warfarin treatment.

ObjectiveIn order to discover the pulsed field gel electrophoresis (PFGE) serotypes and drug resistance of Salmonella agona (S. agona) in Sichuan Province during 2008 to 2018, so as to understand the contamination status of S. agona and provide reference data for outbreak warning, traceability investigation and antibiotic use strategy.MethodsA total of 61 S. agona strains after the serological and biochemical identification were analyzed by PFGE and tested for minimal inhibitory concentration of 14 antibiotics.ResultsThe isolates of S. agona were divided into 41 PFGE types, some strains isolated from clinical cases in different years and regions had the same PFGE type, one pork isolate had the same PFGE type as some clinical isolates. The antibiotic resistance test indicated 28 of the isolates were sensitive to 14 kinds of antibiotics, the other 33 strains showed different degrees of drug resistance, nearly half of the strains were insensitive to tetracycline; and there were 16 multidrug-resistant strains. All of the isolates were sensitive to imipenem.ConclusionThe PFGE types of S. agona isolated in Sichuan from 2008 to 2018 showed diversity, and they were sporadic prevalent in Sichuan Province. The drug resistance was serious with an increasing trend.

Based on the hierarchical model of psychopathology, the theory of compensatory internet use, and the sociocultural self model of behaviour, this study examined whether social anxiety mediated the relationship between neuroticism and problematic online gaming, and whether this mediating process was moderated by family socioeconomic status (FSES). The theoretical model was tested using data collected from 598 Chinese undergraduates (M.sub.age = 19.45 years, SD = 1.63). These participants completed questionnaires anonymously. Testing for moderated mediation revealed that social anxiety partially mediated the association between neuroticism and problematic online gaming, with FSES moderating the second stage of it. Specifically, the indirect effect of neuroticism on problematic online gaming via social anxiety was significant only for undergraduates with low FSES. Results of this study extend research on the influencing factors of problematic online gaming and highlight the significance of uncovering the underlying mechanisms., Author(s): Xiaoyuan Chu [sup.1] [sup.2] [sup.3] , Qi Wang [sup.4] , Weifeng Huang [sup.5] , Yuhui Wang [sup.6] , Jingyue Yu [sup.2] , Yang Zhou [sup.2] , Tiankuo Chen [sup.2] [...]