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373 results on '"Huang Laiqiang"'

3. Nanoparticles of Poly(Lactide-Co-Glycolide)-d-a-Tocopheryl Polyethylene Glycol 1000 Succinate Random Copolymer for Cancer Treatment

Author

Zheng Yi, Yan Fei, Liu Kexin, Tian Ge, Tian Yan, Xu Lei, Ma Yuandong, Huang Laiqiang, and Mei Lin

Subjects
PLGA-TPGS, Random copolymer, Docetaxel, Nanoparticle, HeLa, Cancer chemotherapy, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

Abstract Cancer is the leading cause of death worldwide. Nanomaterials and nanotechnologies could provide potential solutions. In this research, a novel biodegradable poly(lactide-co-glycolide)-d-a-tocopheryl polyethylene glycol 1000 succinate (PLGA-TPGS) random copolymer was synthesized from lactide, glycolide and d-a-tocopheryl polyethylene glycol 1000 succinate (TPGS) by ring-opening polymerization using stannous octoate as catalyst. The obtained random copolymers were characterized by 1H NMR, FTIR, GPC and TGA. The docetaxel-loaded nanoparticles made of PLGA-TPGS copolymer were prepared by a modified solvent extraction/evaporation method. The nanoparticles were then characterized by various state-of-the-art techniques. The results revealed that the size of PLGA-TPGS nanoparticles was around 250 nm. The docetaxel-loaded PLGA-TPGS nanoparticles could achieve much faster drug release in comparison with PLGA nanoparticles. In vitro cellular uptakes of such nanoparticles were investigated by CLSM, demonstrating the fluorescence PLGA-TPGS nanoparticles could be internalized by human cervix carcinoma cells (HeLa). The results also indicated that PLGA-TPGS-based nanoparticles were biocompatible, and the docetaxel-loaded PLGA-TPGS nanoparticles had significant cytotoxicity against Hela cells. The cytotoxicity against HeLa cells for PLGA-TPGS nanoparticles was in time- and concentration-dependent manner. In conclusion, PLGA-TPGS random copolymer could be acted as a novel and promising biocompatible polymeric matrix material applicable to nanoparticle-based drug delivery system for cancer chemotherapy.

Published
2010
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4. A Novel Docetaxel-Loaded Poly (ε-Caprolactone)/Pluronic F68 Nanoparticle Overcoming Multidrug Resistance for Breast Cancer Treatment

Author

Chen Hongli, Tian Ge, Tian Yan, Liu Kexin, Li Zhen, Song Cunxian, Sun Hongfan, Mei Lin, Zhang Yangqing, Zheng Yi, Yang Dongye, and Huang Laiqiang

Subjects
Nanoparticles, MDR, Pluronic F68, Poly (ε-caprolactone), Docetaxel, Breast cancer, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

Abstract Multidrug resistance (MDR) in tumor cells is a significant obstacle to the success of chemotherapy in many cancers. The purpose of this research is to test the possibility of docetaxel-loaded poly (ε-caprolactone)/Pluronic F68 (PCL/Pluronic F68) nanoparticles to overcome MDR in docetaxel-resistance human breast cancer cell line. Docetaxel-loaded nanoparticles were prepared by modified solvent displacement method using commercial PCL and self-synthesized PCL/Pluronic F68, respectively. PCL/Pluronic F68 nanoparticles were found to be of spherical shape with a rough and porous surface. The nanoparticles had an average size of around 200 nm with a narrow size distribution. The in vitro drug release profile of both nanoparticle formulations showed a biphasic release pattern. There was an increased level of uptake of PCL/Pluronic F68 nanoparticles in docetaxel-resistance human breast cancer cell line, MCF-7 TAX30, when compared with PCL nanoparticles. The cytotoxicity of PCL nanoparticles was higher than commercial Taxotere®in the MCF-7 TAX30 cell culture, but the differences were not significant (p > 0.05). However, the PCL/Pluronic F68 nanoparticles achieved significantly higher level of cytotoxicity than both of PCL nanoparticles and Taxotere®(p < 0.05), indicating docetaxel-loaded PCL/Pluronic F68 nanoparticles could overcome multidrug resistance in human breast cancer cells and therefore have considerable potential for treatment of breast cancer.

Published
2009
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6. Diploid and tetraploid genomes of Acorus and the evolution of monocots

Author

Ma, Liang, Liu, Ke-Wei, Li, Zhen, Hsiao, Yu-Yun, Qi, Yiying, Fu, Tao, Tang, Guang-Da, Zhang, Diyang, Sun, Wei-Hong, Liu, Ding-Kun, Li, Yuanyuan, Chen, Gui-Zhen, Liu, Xue-Die, Liao, Xing-Yu, Jiang, Yu-Ting, Yu, Xia, Hao, Yang, Huang, Jie, Zhao, Xue-Wei, Ke, Shijie, Chen, You-Yi, Wu, Wan-Lin, Hsu, Jui-Ling, Lin, Yu-Fu, Huang, Ming-Der, Li, Chia-Ying, Huang, Laiqiang, Wang, Zhi-Wen, Zhao, Xiang, Zhong, Wen-Ying, Peng, Dong-Hui, Ahmad, Sagheer, Lan, Siren, Zhang, Ji-Sen, Tsai, Wen-Chieh, Van de Peer, Yves, and Liu, Zhong-Jian

Published
2023
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7. Oral Delivery of DMAB-Modified Docetaxel-Loaded PLGA-TPGS Nanoparticles for Cancer Chemotherapy

Author

Zeng Xiaowei, Liu Gan, Tian Ge, Tian Yan, Liu Kexin, Li Lei, Li Zhen, Chen Hongbo, Zheng Yi, Mei Lin, and Huang Laiqiang

Subjects
PLGA-TPGS, DMAB, Nanoparticle, Oral Chemotherapy, Docetaxel, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

Abstract Three types of nanoparticle formulation from biodegradable PLGA-TPGS random copolymer were developed in this research for oral administration of anticancer drugs, which include DMAB-modified PLGA nanoparticles, unmodified PLGA-TPGS nanoparticles and DMAB-modified PLGA-TPGS nanoparticles. Firstly, the PLGA-TPGS random copolymer was synthesized and characterized. DMAB was used to increase retention time at the cell surface, thus increasing the chances of particle uptake and improving oral drug bioavailability. Nanoparticles were found to be of spherical shape with an average particle diameter of around 250 nm. The surface charge of PLGA-TPGS nanoparticles was changed to positive after DMAB modification. The results also showed that the DMAB-modified PLGA-TPGS nanoparticles have significantly higher level of the cellular uptake than that of DMAB-modified PLGA nanoparticles and unmodified PLGA-TPGS nanoparticles. In vitro, cytotoxicity experiment showed advantages of the DMAB-modified PLGA-TPGS nanoparticle formulation over commercial Taxotere® in terms of cytotoxicity against MCF-7 cells. In conclusion, oral chemotherapy by DMAB-modified PLGA-TPGS nanoparticle formulation is an attractive and promising treatment option for patients.

Published
2011

9. Genomes of leafy and leafless Platanthera orchids illuminate the evolution of mycoheterotrophy

Author

Li, Ming-He, Liu, Ke-Wei, Li, Zhen, Lu, Hsiang-Chia, Ye, Qin-Liang, Zhang, Diyang, Wang, Jie-Yu, Li, Yu-Feng, Zhong, Zhi-Ming, Liu, Xuedie, Yu, Xia, Liu, Ding-Kun, Tu, Xiong-De, Liu, Bin, Hao, Yang, Liao, Xing-Yu, Jiang, Yu-Ting, Sun, Wei-Hong, Chen, Jinliao, Chen, Yan-Qiong, Ai, Ye, Zhai, Jun-Wen, Wu, Sha-Sha, Zhou, Zhuang, Hsiao, Yu-Yun, Wu, Wan-Lin, Chen, You-Yi, Lin, Yu-Fu, Hsu, Jui-Ling, Li, Chia-Ying, Wang, Zhi-Wen, Zhao, Xiang, Zhong, Wen-Ying, Ma, Xiao-Kai, Ma, Liang, Huang, Jie, Chen, Gui-Zhen, Huang, Ming-Zhong, Huang, Laiqiang, Peng, Dong-Hui, Luo, Yi-Bo, Zou, Shuang-Quan, Chen, Shi-Pin, Lan, Siren, Tsai, Wen-Chieh, Van de Peer, Yves, and Liu, Zhong-Jian

Published
2022
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12. Comparative analysis of Phytophthora genomes reveals oomycete pathogenesis in crops

Author

Gao, Rui-Fang, Wang, Jie-Yu, Liu, Ke-Wei, Yoshida, Kouki, Hsiao, Yu-Yun, Shi, Yi-Xiang, Tsai, Kun-Chan, Chen, You-Yi, Mitsuda, Nobutaka, Liang, Chieh-Kai, Wang, Zhi-Wen, Wang, Ying, Zhang, Di-Yang, Huang, Laiqiang, Zhao, Xiang, Zhong, Wen-Ying, Cheng, Ying-Hui, Jiang, Zi-De, Li, Ming-He, Sun, Wei-Hong, Yu, Xia, Hu, Wenqi, Zhou, Zhuang, Zhou, Xiao-Fan, Yeh, Chuan-Ming, Katoh, Kazutaka, Tsai, Wen-Chieh, Liu, Zhong-Jian, Martin, Francis, and Zhang, Gui-Ming

Published
2021
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16. The Cymbidium genome reveals the evolution of unique morphological traits

Author

Ai, Ye, Li, Zhen, Sun, Wei-Hong, Chen, Juan, Zhang, Diyang, Ma, Liang, Zhang, Qing-Hua, Chen, Ming-Kun, Zheng, Qing-Dong, Liu, Jiang-Feng, Jiang, Yu-Ting, Li, Bai-Jun, Liu, Xuedie, Xu, Xin-Yu, Yu, Xia, Zheng, Yu, Liao, Xing-Yu, Zhou, Zhuang, Wang, Jie-Yu, Wang, Zhi-Wen, Xie, Tai-Xiang, Ma, Shan-Hu, Zhou, Jie, Ke, Yu-Jie, Zhou, Yu-Zhen, Lu, Hsiang-Chia, Liu, Ke-Wei, Yang, Feng-Xi, Zhu, Gen-Fa, Huang, Laiqiang, Peng, Dong-Hui, Chen, Shi-Pin, Lan, Siren, Van de Peer, Yves, and Liu, Zhong-Jian

Published
2021
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17. Correction: The Cymbidium genome reveals the evolution of unique morphological traits

Author

Ai, Ye, Li, Zhen, Sun, Wei-Hong, Chen, Juan, Zhang, Diyang, Ma, Liang, Zhang, Qing-Hua, Chen, Ming-Kun, Zheng, Qing-Dong, Liu, Jiang-Feng, Jiang, Yu-Ting, Li, Bai-Jun, Liu, Xuedie, Xu, Xin-Yu, Yu, Xia, Zheng, Yu, Liao, Xing-Yu, Zhou, Zhuang, Wang, Jie-Yu, Wang, Zhi-Wen, Xie, Tai-Xiang, Ma, Shan-Hu, Zhou, Jie, Ke, Yu-Jie, Zhou, Yu-Zhen, Lu, Hsiang-Chia, Liu, Ke-Wei, Yang, Feng-Xi, Zhu, Gen-Fa, Huang, Laiqiang, Peng, Dong-Hui, Chen, Shi-Pin, Lan, Siren, Van de Peer, Yves, and Liu, Zhong-Jian

Published
2021
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20. A Calcineurin-dependent Switch Controls the Trafficking Function of α-Arrestin Aly1/Art6*

Author

O'Donnell, Allyson F, Huang, Laiqiang, Thorner, Jeremy, and Cyert, Martha S

Subjects
Biochemistry and Cell Biology, Biological Sciences, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, 14-3-3 Proteins, Amino Acid Motifs, Amino Acid Sequence, Arrestins, Calcineurin, Catalytic Domain, Chromatography, Liquid, Endocytosis, Humans, Mass Spectrometry, Models, Biological, Molecular Sequence Data, Mutant Proteins, Phenotype, Phosphopeptides, Phosphorylation, Protein Binding, Protein Stability, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Substrate Specificity, Ubiquitination, Vacuoles, Aly1, Aly2, Arrestin, Calcium, Dip5, Gap1, Nutrient Permeases, Trafficking, Chemical Sciences, Medical and Health Sciences, Biochemistry & Molecular Biology, Biological sciences, Biomedical and clinical sciences, Chemical sciences
Abstract

Proper regulation of plasma membrane protein endocytosis by external stimuli is required for cell growth and survival. In yeast, excess levels of certain nutrients induce endocytosis of the cognate permeases to prevent toxic accumulation of metabolites. The α-arrestins, a family of trafficking adaptors, stimulate ubiquitin-dependent and clathrin-mediated endocytosis by interacting with both a client permease and the ubiquitin ligase Rsp5. However, the molecular mechanisms that control α-arrestin function are not well understood. Here, we show that α-arrestin Aly1/Art6 is a phosphoprotein that specifically interacts with and is dephosphorylated by the Ca(2+)- and calmodulin-dependent phosphoprotein phosphatase calcineurin/PP2B. Dephosphorylation of Aly1 by calcineurin at a subset of phospho-sites is required for Aly1-mediated trafficking of the aspartic acid and glutamic acid transporter Dip5 to the vacuole, but it does not alter Rsp5 binding, ubiquitinylation, or stability of Aly1. In addition, dephosphorylation of Aly1 by calcineurin does not regulate the ability of Aly1 to promote the intracellular sorting of the general amino acid permease Gap1. These results suggest that phosphorylation of Aly1 inhibits its vacuolar trafficking function and, conversely, that dephosphorylation of Aly1 by calcineurin serves as a regulatory switch to promote Aly1-mediated trafficking to the vacuole.

Published
2013

23. The Litsea genome and the evolution of the laurel family

Author

Chen, Yi-Cun, Li, Zhen, Zhao, Yun-Xiao, Gao, Ming, Wang, Jie-Yu, Liu, Ke-Wei, Wang, Xue, Wu, Li-Wen, Jiao, Yu-Lian, Xu, Zi-Long, He, Wen-Guang, Zhang, Qi-Yan, Liang, Chieh-Kai, Hsiao, Yu-Yun, Zhang, Di-Yang, Lan, Si-Ren, Huang, Laiqiang, Xu, Wei, Tsai, Wen-Chieh, Liu, Zhong-Jian, Van de Peer, Yves, and Wang, Yang-Dong

Published
2020
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24. Sophflarine A, a novel matrine-derived alkaloid from Sophora flavescens with therapeutic potential for non-small cell lung cancer through ROS-mediated pyroptosis and autophagy

Author

Luo, Ding, primary, Dai, Xiaoyong, additional, Tian, He, additional, Fan, Chunlin, additional, Xie, Huayan, additional, Chen, Nenghua, additional, Wang, Jinghao, additional, Huang, Laiqiang, additional, Wang, Hao, additional, Wang, Guocai, additional, and Zhang, Yubo, additional

Published
2023
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28. A Self‐Reinforcing Nanoplatform for Highly Effective Synergistic Targeted Combinatary Calcium‐Overload and Photodynamic Therapy of Cancer

Author

Guo, Dongdong, primary, Dai, Xiaoyong, additional, Liu, Kewei, additional, Liu, Yuhong, additional, Wu, Jiamin, additional, Wang, Kun, additional, Jiang, Shengwei, additional, Sun, Fen, additional, Wang, Lijun, additional, Guo, Bing, additional, Yang, Dongye, additional, and Huang, Laiqiang, additional

Published
2023
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29. NIR-IIb fluorescence-image guided synergistic surgery/starvation/chemodynamic therapy: an innovative treatment paradigm for malignant non-small cell lung cancers

Author

Han, Xuejiao, primary, Zhong, Yingtao, additional, Mi, Chao, additional, He, Zhiguo, additional, Gu, Jingsi, additional, Dai, Xiaoyong, additional, Ma, Chenguang, additional, Feng, Chunyan, additional, Chen, Huaqing, additional, Lan, Zebin, additional, Guo, Zhiyong, additional, Huang, Laiqiang, additional, Zhang, Baozhu, additional, Guo, Bing, additional, and Meng, Qingwei, additional

Published
2023
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31. The Annona montana genome reveals the development and flavor formation in mountain soursop fruit

Author

Tang, Guangda, primary, Chen, Guizhen, additional, Ke, Jianhao, additional, Wang, Jieyu, additional, Zhang, Diyang, additional, Liu, Dingkun, additional, Huang, Jie, additional, Zeng, Sijin, additional, Liao, Miao, additional, Wei, Xuefen, additional, Huang, Zihao, additional, Ou, Minghui, additional, Zeng, Jian, additional, Wu, Hao, additional, Zheng, Jie, additional, Liu, Kewei, additional, Sun, Weihong, additional, Liu, Xuedie, additional, Yu, Xia, additional, Xu, Xinyu, additional, Zhao, Xuewei, additional, Li, Yuanyuan, additional, Zhao, Zhuang, additional, Chen, Deqiang, additional, Zheng, Qinyao, additional, He, Xin, additional, Zhang, Mengmeng, additional, Huang, Ye, additional, Zhang, Cuili, additional, Li, Minghe, additional, Wang, Zhiwen, additional, Ahmad, Sagheer, additional, Chang, Shengxin, additional, Zou, Shuangquan, additional, Huang, Laiqiang, additional, Peng, Donghui, additional, Lan, Siren, additional, and Liu, Zhongjian, additional

Published
2023
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48. PHF6 functions as a tumor suppressor by recruiting methyltransferase SUV39H1 to nucleolar region and offers a novel therapeutic target for PHF6-muntant leukemia

Author

Tsai, Hsiang-i, primary, Wu, Yanping, additional, Huang, Rui, additional, Su, Dandan, additional, Wu, Yingyi, additional, Liu, Xiaoyan, additional, Wang, Linglu, additional, Xu, Zhanxue, additional, Pang, Yuxin, additional, Sun, Chong, additional, He, Chao, additional, Shu, Fan, additional, Zhu, Haitao, additional, Wang, Dongqing, additional, Cheng, Fang, additional, Huang, Laiqiang, additional, and Chen, Hongbo, additional

Published
2022
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49. Additional file 1 of Peptide-based PROTAC degrader of FOXM1 suppresses cancer and decreases GLUT1 and PD-L1 expression

Author

Wang, Kun, Dai, Xiaoyong, Yu, Albert, Feng, Chunyan, Liu, Kewei, and Huang, Laiqiang

Abstract

Additional file 1: Supplementary Fig. 1. Analysis of peptide screening results and molecular docking. A, Second round result of screening FOXM1 targeted peptides using an in vitro phage display. B, Third round result of screening FOXM1 targeted peptides using an in vitro phage display. C, Peptide characteristic analysis. d, Molecular docking of FIP-1 and FOXM1-PROTAC with Pymol. Supplementary Fig. 2. Western blotting results. A, MS detection of FIP-1 and FOXM1-PROTAC. B, Western blotting examination for FoxM1 of HepG2 cells treated with 20 μM FIP-1 for different time (0, 3, 6, 12, 24, 48 h). C, Western blotting test for FoxM1 of HepG2 cells treated for 24 h with increasing concentrations of FIP-1 (0, 2, 5, 10, 20, 30, 50 μM). Supplementary Fig. 3. FOXM1-PROTAC inhibits proliferation of HepG2 and MDA-MB-231 cells in vitro. A, The raw data of DNA content of HepG2 and MDA-MB-231cells on a Flow cytometer, treated with FIP-1 and FOXM1-PROTAC for 24 h and stained with propidium iodide. B, The examination of CDK1, CyclinB1 and CDC25B level of HepG2 and MDA-MB-231 cells, treated with FIP-1 and FOXM1-PROTAC for 48 h, using Western blotting. Supplementary Fig. 4. Toxicity test of FOXM1-PROTAC in vivo. A, Changes of tumor volume and statistical diagram of tumor weight. B, Changes of body weight of nude mice after caudal vein injection. C, Immunohistochemistry of Heart, Liver, Spleen, Lung and Kidney Treated by FIP-1 and FOXM1-PROTAC for 14 Days (20 mg/kg) . D, E, Activities of serum aspartic acid transferase (AST), creatinine and Blood urea nitrogen did not elevate or reduce significantly both in mice injecting with HepG2 and MDA-MB-231 cells. Supplementary Fig. 5. The uptake of 2-NBDG in HepG2 cells. A, The raw data of 2-NBDG fluorescence intensity in HepG2 cells on a Flow cytometer. Cells were treated with FIP-1 and FOXM1-PROTAC for 24 h and 2-NBDG for 30 min. B, The fluorescence of 2-NBDG in HepG2 cells imaged with the fluorescent Con-focal microscope. Cells were treated with FIP-1 and FOXM1-PROTAC for 24 h and 2-NBDG for 30 min. C, The PD-L1 on the membrane of HepG2 cells, treated with FIP-1 or FOXM1-PROTAC, was determined by flow cytometry.

Published
2022
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