Search

Your search keyword '"Huang, Zonghai"' showing total 142 results
Start Over Author "Huang, Zonghai"
142 results on '"Huang, Zonghai"'

3. Network Analysis of Osteoarthritis Progression Using a Steiner Minimal Tree Algorithm.

Author

Xie, Yujie, Shao, Fanglin, Ji, Yuxiu, Feng, Dechao, Wang, Ling, Huang, Zonghai, Wu, Shengjian, Sun, Fuhua, Jiang, Hong, Miyamoto, Akira, Wang, Haiming, and Zhang, Chi

Subjects
OSTEOARTHRITIS, CHONDROGENESIS, BIOMOLECULES, ALGORITHMS, MEDICAL rehabilitation
Abstract

aiming Wang,6 Chi Zhang11Rehabilitation Medicine Department, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, People's Republic of China; 2Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, People's Republic of China; 3School of Information and Communication Engineering, University of Electronic Science and Technology, Chengdu, Sichuan, People's Republic of China; 4Rehabilitation Medicine Department, Xichong County People's hospital, Nanchong, Sichuan, People's Republic of China; 5Faculty of Rehabilitation, Nishikyushu University, Kansaitama, Japan; 6Department of Rehabilitation Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China Correspondence: Haiming Wang, Department of Rehabilitation Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, People's Republic of China, Tel +868950771, Fax +86 830 8950771, Email [email protected] Chi Zhang, Rehabilitation Medicine Department, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, People's Republic of China, Email [email protected] Purpose: To provide a comprehensive analysis of associated genes with osteoarthritis (OA). Here, we reported a network analysis of OA progression by using a Steiner minimal tree algorithm. Methods: We collected the OA-related genes through screening the publications in MEDLINE. We performed functional analysis to analyze the associated biochemical pathways of the OA-related genes. Pathway crosstalk analysis was constructed to explore interactions of the enriched pathways. Steiner minimal tree algorithm was used to analyze molecular pathway networks. The average clustering coefficient was compared with the corresponding values of the Osteoarthritis-specific network. The new finding RNA was compared with former single-cell RNA-seq analysis results. Results: A gene set with 177 members reported to be significantly associated with Osteoarthritis was collected from 187 studies. Functional enrichment analysis revealed a specific related-OA gene including skeletal system development, cytokine-mediated signaling pathway, inflammatory response, cartilage development, and extracellular matrix organization. We performed a pathway crosstalk analysis among the 72 significantly enriched pathways. A total of 151 of the 177 genes in the Osteoarthritis gene set were included in the human interactome network. There were 31 genes in the former single-cell RNA-seq analysis results. The CLU, ENO1, SRRM1, UBC, HMGB1, NR3C1, NOTCH2NL, and CBX5 have significantly increased expression in seven molecularly defined populations of OA cartilage. Conclusion: The Steiner tree-based approach finds new biological molecules associated with OA genes. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

14. Corrigendum to “Epigenetic silencing of miR-490-3p promotes development of an aggressive colorectal cancer phenotype through activation of the Wnt/β-catenin signaling pathway”. Cancer Letters, 376 (2016) 178-87

Author

Zheng, Kehong, primary, Zhou, Xinying, additional, Yu, Jinlong, additional, Li, Qiang, additional, Wang, Hui, additional, Li, Mingyi, additional, Shao, Ziyun, additional, Zhang, Feifei, additional, Luo, Yuhao, additional, Shen, Zetao, additional, Chen, Fei, additional, Shi, Fujun, additional, Cui, Chunhui, additional, Zhao, Dachuan, additional, Lin, Zhiqun, additional, Zheng, Wei, additional, Zou, Zhaowei, additional, Huang, Zonghai, additional, and Zhao, Liang, additional

Published
2021
Full Text
View/download PDF

15. Selenium nanoparticles coated with pH responsive silk fibroin complex for fingolimod release and enhanced targeting in thyroid cancer

Author

Zou, Xiangcai, Jiang, Zhipeng, Li, Liang, and Huang, Zonghai

Subjects
inorganic chemicals, hemic and lymphatic diseases, technology, industry, and agriculture, respiratory system, health care economics and organizations
Abstract

Cancer-targeted drug delivery systems based on nanoparticles (NPs) have been considered promising therapies. In this study, we developed a pH-responsive smart NPs drug delivery system using silk fibroin (SF), selenium nanoparticles (Se NPs), fingolimod (FTY720), and heptapeptide (T7). The prepared FTY720@T7-SF-Se NPs were spheres with an average diameter of 120 nm, which would contribute to the enhanced permeability and retention effects in tumour regions. The encapsulation efficiency (EE) of the FTY720@T7-SF-Se NPs was 71.95 ± 3.81%. The release of FTY720 from the nanocarriers was pH-dependent, and the release of FTY720 was accelerated in an acidic environment. Both in vitro and in vivo studies showed that FTY720@T7-SF-Se NPs had an enhanced cellular uptake selectivity and antitumor activity for thyroid cancer. The bio-distribution study in vivo further demonstrated that FTY720@T7-SF-Se NPs could effectively accumulate in the tumour region, thereby enhancing the ability to kill cancer cells in vivo. In addition, studies of histology and immunohistochemistry showed that FTY720@T7-SF-Se NPs had low toxicity to the major organs of tumour-bearing mice, indicating the prepared NPs has good biocompatibility in vivo. These results suggest that the tumour-targeted NPs delivery system (FTY720@T7-SF-Se NPs) has great potential as a new tool for thyroid cancer therapy.

Published
2021
Full Text
View/download PDF

23. Neoadjuvant Modified FOLFOX6 With or Without Radiation Versus Fluorouracil Plus Radiation for Locally Advanced Rectal Cancer: Final Results of the Chinese FOWARC Trial

Author

Deng, Yanhong, primary, Chi, Pan, additional, Lan, Ping, additional, Wang, Lei, additional, Chen, Weiqing, additional, Cui, Long, additional, Chen, Daoda, additional, Cao, Jie, additional, Wei, Hongbo, additional, Peng, Xiang, additional, Huang, Zonghai, additional, Cai, Guanfu, additional, Zhao, Ren, additional, Huang, Zhongcheng, additional, Xu, Lin, additional, Zhou, Hongfeng, additional, Wei, Yisheng, additional, Zhang, Hao, additional, Zheng, Jian, additional, Huang, Yan, additional, Zhou, Zhiyang, additional, Cai, Yue, additional, Kang, Liang, additional, Huang, Meijin, additional, Wu, Xiaojian, additional, Peng, Junsheng, additional, Ren, Donglin, additional, and Wang, Jianping, additional

Published
2019
Full Text
View/download PDF

26. Modified FOLFOX6 with or without radiation in neoadjuvant treatment of locally advanced rectal cancer: Final results of the Chinese FOWARC multicenter randomized trial.

Author

Deng, Yanhong, primary, Chi, Pan, additional, Lan, Ping, additional, Wang, Lei, additional, Chen, Weiqing, additional, Cui, Long, additional, Chen, Daoda, additional, Cao, Jie, additional, Wei, Hongbo, additional, Peng, Xiang, additional, Huang, Zonghai, additional, Cai, Guanfu, additional, Zhao, Ren, additional, Huang, Zhongcheng, additional, Luo, Yiding, additional, Zhou, Hongfeng, additional, Wei, Yisheng, additional, Zhang, Hao, additional, Zheng, Jian, additional, and Wang, Jianping, additional

Published
2018
Full Text
View/download PDF

27. Study of the Mechanism of Bystander Effect of KDR-CDglyTK System Mediated by Adenovirus for the Treatment of Gastric Cancer

Author

Chen Fei, Li Zhou, Huang Zonghai, Li Qiang, Li Yanping, and Yu Jinlong

Subjects
Antimetabolites, Antineoplastic, Cell Survival, viruses, Genetic Vectors, Green Fluorescent Proteins, Biophysics, Cell Communication, Transfection, Antiviral Agents, Biochemistry, Adenoviridae, HeLa, chemistry.chemical_compound, Stomach Neoplasms, Cell Line, Tumor, Bystander effect, Humans, Apigenin, Promoter Regions, Genetic, Ganciclovir, biology, Fluorescence recovery after photobleaching, Bystander Effect, Genetic Therapy, Cell Biology, General Medicine, Suicide gene, biology.organism_classification, Molecular biology, Cell killing, chemistry, Cell culture, Fluorouracil, Fluorescence Recovery After Photobleaching, HeLa Cells
Abstract

We evaluated the relationship between cellular gap junction and bystander effect in gastric cancer SCG7901 cell killing by adenovirus-mediated KDR-CDglyTK system. SCG7901 and HeLa cells were transfected (MOI-100) with recombinant adenovirus carrying fusion gene. Transfection efficiency/fusion gene mRNA expression were determined; intercellular communications in the presence of apigenin were measured by fluorescence recovery after photobleaching (FRAP). Transfected/non-transfected cells were mixed at 5:95/10:90% ratios, respectively, incubated for 24 h with/without apigenin, and for 72 h with GCV and 5-FU. Cell survival was determined by MTT method. The transfected SCG7901/HeLa cells expressed GFP. Fluorescence intensity in SCG7901 cells decreased after photobleaching but recovered over time. Fluorescence intensity in HeLa cells also decreased after photobleaching with no recovery. Following apigenin treatment, fluorescence intensity differed between SCG7901 and HeLa cells. Survival rates of SCG7901 cells differed among prodrug, apigenin, and prodrug + apigenin groups compared with controls, but no significant difference was observed for HeLa cells. We concluded that the intercellular communication in SCG7901 cells related with the gap junctions, while there was no such communication in HeLa cells. The bystander killing effect related with the intercellular gap junctions and was increased by apigenin with no such effect seen in HeLa cells.

Published
2013

28. Impact of platelet to lymphocyte ratio and metabolic syndrome on the prognosis of colorectal cancer patients

Author

You,Jie, Zhang,Huxiang, Shen,Yanyan, Chen,Chuanzhi, Liu,Wenyue, Zheng,Minghua, Poucke,Sven Van, Guo,Guilong, Huang,Zonghai, You,Jie, Zhang,Huxiang, Shen,Yanyan, Chen,Chuanzhi, Liu,Wenyue, Zheng,Minghua, Poucke,Sven Van, Guo,Guilong, and Huang,Zonghai

Abstract

Jie You,1,2,* Huxiang Zhang,3,* Yanyan Shen,2,* Chuanzhi Chen,2 Wenyue Liu,4 Minghua Zheng,5 Sven Van Poucke,6 Guilong Guo,2 Zonghai Huang1 1Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 2Department of Oncological Surgery, 3Department of Pathology, 4Department of Endocrinology, 5Department of Hepatology, Liver Research Center, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People’s Republic of China; 6Department of Anesthesiology, Intensive Care, Emergency Medicine and Pain Therapy, Ziekenhuis Oost-Limburg, Genk, Belgium *These authors contributed equally to this work Objective: The aim of this study was to evaluate the prognostic value of both platelet to lymphocyte ratio (PLR) and metabolic syndrome (MetS) in colorectal cancer (CRC) patients.Patients and methods: We retrospectively enrolled 1,163 CRC patients. Preoperative values of PLR were stratified into three groups according to cut-off values of 120 and 220. The Kaplan–Meier analysis was used to calculate cumulative survival rate related to PLR and MetS. Cox proportional hazard regression models were used to analyze potential risk factors and the prognosis associated with PLR and MetS in CRC patients.Results: PLR was significantly higher in the MetS(+) group as compared to MetS(–) group (P=0.039). An elevated PLR was significantly associated with mortality (P=0.014), but not the existence of MetS (P=0.235). In multivariate regression analysis, PLR was an independent risk factor for overall survival (OS) (P=0.046). For the subgroup with a PLR >220, MetS was an independent predictor for both OS and disease-free survival (P=0.039 and P=0.047, respectively) by multivariate analysis adjusting for confounding covariates. In addition, the presence of MetS was associated with a 2-fold increased risk of mortality and tumor recurrences (hazard ratio [HR] =2.0 and HR =1.9, P<0.05, respectively).Conclusion: Preo

Published
2017

29. Preparation of Cold Brew Tea by Explosion Puffing Drying at Variable Temperature and Pressure

Author

Huang Zonghai, Jin-Fu Liu, and Xin-Yi He

Subjects
General Chemical Engineering, technology, industry, and agriculture, food and beverages, Free amino, Green tea, complex mixtures, chemistry.chemical_compound, Temperature and pressure, chemistry, Polyphenol, Food science, Physical and Theoretical Chemistry, Caffeine, Water content, Tea leaf
Abstract

Cold brew tea was prepared using explosion puffing drying at variable temperature and pressure. The influences of moisture content of predried tea leaves, freezing pretreatment times at −18°C, and puffing temperature on water extracts content of cold brew tea were studied according to the orthogonal experiments of processing of cold brew tea based on single factors. The biochemistry ingredients of cold brew tea and hot air–dried green tea were determined and compared. The optimum processing conditions of cold brew tea were obtained as follows: predried tea leaf moisture content 50%, freezing pretreatment three times, puffing temperature 105°C. Under these optimized processing conditions, cold brew tea was obtained. When cold brew tea was soaked in cold water for 30 min at 20°C, the water extracts content, caffeine, tea polyphenols, and free amino acids of cold brew tea reached 18.21, 0.88, 11.86, and 1.02%, respectively. High-performance liquid chromatography (HPLC) analysis showed that the amount of tea ...

Published
2011

32. Two SNPs of ATP-binding cassette B1 gene on the risk and prognosis of colorectal cancer

Author

Wang, Fei, Huang, Zonghai, Zheng, Kehong, Zhao, Haiping, and Hu, Wenxiu

Subjects
Adult, Male, ATP Binding Cassette Transporter, Subfamily B, Genotype, Kaplan-Meier Estimate, Middle Aged, Prognosis, Polymorphism, Single Nucleotide, Case-Control Studies, Humans, Original Article, Female, Genetic Predisposition to Disease, Colorectal Neoplasms, Polymorphism, Restriction Fragment Length, Aged, Proportional Hazards Models
Abstract

We conducted a case-control study to investigate the role of ABCB1 C3435T and G2677T/A in the susceptibility and prognosis of colorectal cancer patients. A total of 316 patients with colorectal cancer and 316 controls were collected between January 2009 and January 2011. Genotyping of ABCB1 C3435T and G2677T/A was conducted by the methods of Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Conditional logistic regression analysis showed that subjects carrying CT and CC genotypes of ABCB1 C3435T were more frequently observed in colorectal cancer patients when compared with controls, and the adjusted ORs were 1.62 (1.05-2.52) and 2.05 (1.25-3.36), respectively. By Cox regression analysis, we found that the TT genotype of ABCB1 C3435T was significantly associated with shorter PFS and OS in patients with colorectal cancer when compared with CC genotype, with adjusted HR (95% CI) of 2.57 (1.14-6.04) and 2.54 (1.05-6.61), respectively. We found that the ABCB1 C3435T polymorphism could affect the susceptibility and clinical outcome of colorectal cancer patients.

Published
2015

34. Modified FOLFOX6 With or Without Radiation Versus Fluorouracil and Leucovorin With Radiation in Neoadjuvant Treatment of Locally Advanced Rectal Cancer: Initial Results of the Chinese FOWARC Multicenter, Open-Label, Randomized Three-Arm Phase III Trial

Author

Deng, Yanhong, primary, Chi, Pan, additional, Lan, Ping, additional, Wang, Lei, additional, Chen, Weiqing, additional, Cui, Long, additional, Chen, Daoda, additional, Cao, Jie, additional, Wei, Hongbo, additional, Peng, Xiang, additional, Huang, Zonghai, additional, Cai, Guanfu, additional, Zhao, Ren, additional, Huang, Zhongcheng, additional, Xu, Lin, additional, Zhou, Hongfeng, additional, Wei, Yisheng, additional, Zhang, Hao, additional, Zheng, Jian, additional, Huang, Yan, additional, Zhou, Zhiyang, additional, Cai, Yue, additional, Kang, Liang, additional, Huang, Meijin, additional, Peng, Junsheng, additional, Ren, Donglin, additional, and Wang, Jianping, additional

Published
2016
Full Text
View/download PDF

37. Anti-Warburg effect of rosmarinic acid via miR-155 in gastric cancer cells

Author

Han,Shuai, Yang,Shaohua, Cai,Zhai, Pan,Dongyue, Li,Zhou, Huang,Zonghai, Zhang,Pusheng, Zhu,Huijuan, Lei,Lijun, Wang,Weiwei, Han,Shuai, Yang,Shaohua, Cai,Zhai, Pan,Dongyue, Li,Zhou, Huang,Zonghai, Zhang,Pusheng, Zhu,Huijuan, Lei,Lijun, and Wang,Weiwei

Abstract

Shuai Han,* Shaohua Yang,* Zhai Cai, Dongyue Pan, Zhou Li, Zonghai Huang, Pusheng Zhang, Huijuan Zhu, Lijun Lei, Weiwei Wang Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, People’s Republic of China *These authors contributed equally to this study Background: The Warburg effect refers to glycolytic production of adenosine triphosphate under aerobic conditions, and is a universal property of most cancer cells. Chronic inflammation is a key factor promoting the Warburg effect. This study aimed to determine whether rosmarinic acid (RA) has an anti-Warburg effect in gastric carcinoma in vitro and in vivo. The mechanism for the anti-Warburg effect was also investigated.Methods: An MTT assay was used to examine MKN45 cell growth in vitro. An enzyme-linked immunosorbent assay was used to detect proinflammatory cytokines. Real-time polymerase chain reaction was used to evaluate levels of microRNA expression in cells. Protein expression was determined by Western blotting assay. Mouse xenograft models were established using MKN45 cells to assess the anti-Warburg effect in gastric carcinoma in vivo.Results: RA suppressed glucose uptake and lactate production. It also inhibited expression of transcription factor hypoxia-inducible factor-1α, which affects the glycolytic pathway. Inflammation promoted the Warburg effect in cancer cells. As expected, RA inhibited proinflammatory cytokines and microRNAs related to inflammation, suggesting that RA may suppress the Warburg effect via an inflammatory pathway, such as that involving interleukin (IL)-6/signal transducer and activator of transcription-3 (STAT3). miR-155 was found to be an important mediator in the relationship between inflammation and tumorigenesis. We further showed that miR-155 was the target gene regulating the Warburg effect via inactivation of the IL-6/STAT3 pathway. Moreover, we found that RA suppressed the Warburg effect in vivo.Conclusion: RA

Published
2015

42. A multi-center randomized controlled trial of mFOLFOX6 with or without radiation in neoadjuvant treatment of local advanced rectal cancer (FOWARC study): Preliminary results.

Author

Deng, Yanhong, primary, Chi, Pan, additional, Lan, Ping, additional, Wang, Lei, additional, Cui, Long, additional, Chen, Daoda, additional, Cao, Jie, additional, Wei, Hongbo, additional, Peng, Xiang, additional, Huang, Zonghai, additional, Cai, Guangfu, additional, Zhao, Ren, additional, Huang, Zhongcheng, additional, Luo, Yiding, additional, Zhang, Yuxin, additional, Cai, Yue, additional, and Wang, Jianping, additional

Published
2015
Full Text
View/download PDF

50. FAM172A is a tumor suppressor in colorectal carcinoma.

Author

Cui, Chunhui, Ye, Lili, Huang, Zonghai, Huang, Shuxin, Liu, Hao, and Yu, Jinlong

Abstract

The present study was designed to elucidate the regulatory role of a novel protein FAM172A in carcinogenesis of colorectal carcinoma (CRC). Investigation of clinical samples using Western blotting showed that expression of FAM172A is significantly lower in cancerous tissues than in adjacent tissues. Furthermore, we constructed in vitro model for continuous overexpression and silencing of FAM172A with a retroviral vector system. FAM172A suppressed the proliferative and invasive potentials of LOVO cells as shown in MTT test, transwell migration assay, wound healing assay, 3D-culture morphologic study, and xenograft experiment. RT-PCR and Western blotting showed that FAM172A overexpression inhibited expressions of Cyclin D1, CDK2, MMP-2, MMP-9, PERK, elF2α, ATF6, XBP1, and GRP78, while FAM172A silencing induced their expressions. FAM172A might regulate ERS through PERK-elF2α, ATF6-XBP1-GRP78 signal pathway. The results implicated that FAM172A functioned as a tumor suppressor in colorectal carcinoma. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results