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25 results on '"Huang, Yongcai"'

9. Maize DDK1 encoding an Importin‐4 β protein is essential for seed development and grain filling by mediating nuclear exporting of eIF1A.

Author

Huang, Xing, Huang, Yongcai, Qin, Li, Xiao, Qiao, Wang, Qiong, Wang, Jiechen, Wang, Wenqin, Lu, Xiaoduo, and Wu, Yongrui

Subjects
*SEED development, *NUCLEAR membranes, *IMMOBILIZED proteins, *EUKARYOTIC cells, *PROTEIN synthesis, *CORN
Abstract

Summary: Nuclear‐cytoplasmic trafficking is crucial for protein synthesis in eukaryotic cells due to the spatial separation of transcription and translation by the nuclear envelope. However, the mechanism underlying this process remains largely unknown in plants.In this study, we isolated a maize (Zea mays) mutant designated developmentally delayed kernel 1 (ddk1), which exhibits delayed seed development and slower filling. Ddk1 encodes a plant‐specific protein known as Importin‐4 β, and its mutation results in reduced 80S monosomes and suppressed protein synthesis.Through our investigations, we found that DDK1 interacts with eIF1A proteins in vivo. However, in vitro experiments revealed that this interaction exhibits low affinity in the absence of RanGTP. Additionally, while the eIF1A protein primarily localizes to the cytoplasm in the wild‐type, it remains significantly retained within the nuclei of ddk1 mutants. These observations suggest that DDK1 functions as an exportin and collaborates with RanGTP to facilitate the nuclear export of eIF1A, consequently regulating endosperm development at the translational level.Importantly, both DDK1 and eIF1A are conserved among various plant species, implying the preservation of this regulatory module across diverse plants. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Teosinte high protein 9 enhances the seed protein content and nitrogen utilization efficiency in maize

Author

Wu, Yongrui, primary, Huang, Yongcai, additional, Wang, Haihai, additional, Zhu, Yidong, additional, Huang, Xing, additional, Li, Shuai, additional, Wu, Xingguo, additional, Zhao, Yao, additional, Bao, Zhigui, additional, Qin, Li, additional, Jin, Yongbo, additional, Cui, Yahui, additional, Ma, Guangjin, additional, Xiao, Qiao, additional, Wang, Qiong, additional, Wang, Jiechen, additional, Yang, Xuerong, additional, Liu, Hongjun, additional, Lu, Xiaoduo, additional, Larkins, Brian, additional, and Wang, Wenqin, additional

Published
2022
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18. High-throughput identification and marker development of perfect SSR for cultivated genus of passion fruit (Passiflora edulis)

Author

Xinghai Yang, Liu Jieyun, Xiuzhong Xia, Huang Weihua, Huang Yongcai, Haifei Mou, Tian Qinglan, and Wu Yanyan

Subjects
Passiflora, Molecular breeding, Germplasm, Horticulture, biology, Genus, Genomics, Identification (biology), Cultivar, biology.organism_classification, Genome
Abstract

Simple sequence repeat (SSR) markers are characterized by high polymorphism, good reproducibility and co-dominance etc. They can be easily applied to develop efficient, simple and practical molecular markers. In the present study, bioinformatics methods were applied to identify high-throughput perfect SSRs of cultivar Passiflora genome. A total of 13104 perfect SSRs were obtained. SSR core sequence structure is mainly 2-4 bases, the maximum numbers are TA, AT, TC and AG. The maximum numbers of repetitions were up to 20 times. A total of 12934 pairs of SSR markers were developed by using bioinformatics software, and 20 pairs of markers were selected for amplification specificity assessment of MTX and WJ10, and the polymorphism rate was as high as 60%. The large-scale development of the SSR markers of Passiflora cultivar has paved a foundation for the efficient utilization of the germplasm resources of passion fruit, genetic improvement of the varieties and molecular breeding.

Published
2018
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21. THP9enhances seed protein content and nitrogen-use efficiency in maize

Author

Huang, Yongcai, Wang, Haihai, Zhu, Yidong, Huang, Xing, Li, Shuai, Wu, Xingguo, Zhao, Yao, Bao, Zhigui, Qin, Li, Jin, Yongbo, Cui, Yahui, Ma, Guangjin, Xiao, Qiao, Wang, Qiong, Wang, Jiechen, Yang, Xuerong, Liu, Hongjun, Lu, Xiaoduo, Larkins, Brian A., Wang, Wenqin, and Wu, Yongrui

Abstract

Teosinte, the wild ancestor of maize (Zea mayssubsp. mays), has three times the seed protein content of most modern inbreds and hybrids, but the mechanisms that are responsible for this trait are unknown1,2. Here we use trio binning to create a contiguous haplotype DNA sequence of a teosinte (Zea mayssubsp. parviglumis) and, through map-based cloning, identify a major high-protein quantitative trait locus, TEOSINTE HIGH PROTEIN 9(THP9), on chromosome 9. THP9encodes an asparagine synthetase 4 enzyme that is highly expressed in teosinte, but not in the B73 inbred, in which a deletion in the tenth intron of THP9-B73causes incorrect splicing of THP9-B73transcripts. Transgenic expression of THP9-teosintein B73 significantly increased the seed protein content. Introgression of THP9-teosinteinto modern maize inbreds and hybrids greatly enhanced the accumulation of free amino acids, especially asparagine, throughout the plant, and increased seed protein content without affecting yield. THP9-teosinteseems to increase nitrogen-use efficiency, which is important for promoting a high yield under low-nitrogen conditions.

Published
2022
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24. The genetic architecture of amylose biosynthesis in maize kernel.

Author

Li, Changsheng, Huang, Yongcai, Huang, Ruidong, Wu, Yongrui, and Wang, Wenqin

Subjects
*AMYLOSE, *AMYLOPECTIN, *TRANSCRIPTION factors, *FOOD industry, CORN genetics
Abstract

Summary: Starch is the most abundant storage carbohydrate in maize kernel. The content of amylose and amylopectin confers unique properties in food processing and industrial application. Thus, the resurgent interest has been switched to the study of individual amylose or amylopectin rather than total starch, whereas the enzymatic machinery for amylose synthesis remains elusive. We took advantage of the phenotype of amylose content and the genotype of 9,007,194 single nucleotide polymorphisms from 464 inbred maize lines. The genome‐wide association study identified 27 associated loci involving 39 candidate genes that were linked to amylose content including transcription factors, glycosyltransferases, glycosidases, as well as hydrolases. Except the waxy gene that encodes the granule‐bound starch synthase, the remaining candidate genes were located in the upstream pathway of amylose synthesis, while the downstream members were already known from prior studies. The linked candidate genes could be transferred to manipulate amylose content and thus add value to maize kernel in the breeding programme. [ABSTRACT FROM AUTHOR]

Published
2018
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25. Tissue culture of Sophora tonkinensis Gapnep. and its quality evaluation

Author

Li Cui, Wang Mei-Ying, Li Linxuan, Huang YongCai, Wei Kunhua, and Miao Jianhua

Subjects
micropropagation, biology, Plant tissue culture, Sophora tonkinensis, food and beverages, Pharmaceutical Science, biology.organism_classification, quality evaluation, tissue culture plant, chemistry.chemical_compound, Horticulture, Tissue culture, Murashige and Skoog medium, chemistry, Matrine, Micropropagation, Matrine and oxymatrine, Drug Discovery, Botany, Original Article, Kinetin, Sophora tonkinensis Gapnep, Explant culture
Abstract

Background: Sophora tonkinensis Gapnep. is an important rare medicinal plant in China. There were only a few papers on the rapid propagation of S. tonkinensis through in vitro tissue culture, and still no report focuses on the quality analysis of in vitro tissue culture plantlets. Materials and Methods: The different concentrations of 6-benzylaminopurine (BAP), kinetin (KT), and indole-3-acetic acid (IAA) were used to establish and screen the optimal rapid propagation technology of S. tonkinensis by orthogonal test; the different concentrations of a-naphthalene acetic acid (NAA), indole-3-butyric acid (IBA), and ABT rooting power (ABT) were used to screen the optimal rooting technology. For quality evaluation of tissue culture plants, three different sites were chose to finish planting experiment. The leaf characteristics, radix ex rhizoma yield, and contents of matrine and oxymatrine were evaluated, respectively, to provide evidence of high yield and good qualities of tissue culture plants. Results: A large number of buds could be induced directly from epicotyl and hypocotyl explants on the Murashige and Skoog (MS) medium supplemented with 1.5 mg/l BAP, 0.5 mg/l IAA, and 0.5 mg/l KT; the best root induction medium was solid MS medium at half the macronutrient concentration supplemented with 1.0 mg/l NAA, 0.4 mg/l IBA, and 0.1 mg/l ABT. The rooting rate was 98%. All tissue culture plants showed normal leaf characteristics. Tissue culture plants from two sites possessed higher radix ex rhizoma yield and overall productivity of matrine and oxymatrine than those of seed plants. Conclusion: Tissue culture is a rapid, effective, and convenient propagation method for S. tonkinensis, and the quality of S. tonkinensis tissue culture plants meets the requirement of quality standard of China Pharmacopoeia (edition 2010), the crude drug from S. tonkinensis tissue culture plants will be suitable for substituting the crude drug from seed plants.

Published
2013

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