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40 results on '"Huang, Yishun"'

4. Correlation Analysis of Porosity and Uniaxial Compressive Strength of Jurassic Frozen Sandstone

Author

HUANG Zhaomei, CAO Guangyong, CHENG Hua, MA Maoyan, HUANG Yishun

Subjects
porosity, nuclear magnetic resonance, sandstone, uniaxial compressive strength, temperature, freezing method, Mining engineering. Metallurgy, TN1-997
Abstract

To study the relationship between rock porosity and uniaxial compressive strength under freezing conditions, NMR and uniaxial compression tests were performed on Jurassic saturated aquifer sandstone under artificial freezing conditions. The relationship between temperature and uniaxial compressive strength and porosity under different temperature conditions was analyzed, and the porosity and uniaxial compressive strength were correlated. The results show that: the uniaxial compressive strength and temperature of sandstone satisfy the quadratic curve relationship; from -5 ℃ to -15 ℃, the increase value in uniaxial compressive strength decreases from 4.54 MPa to 0.54 MPa. The axial compressive strength growth rate decreased from 14.22% to 1.33%; the porosity of saturated frozen sandstone decreases with temperature, and its porosity also decreases, and the relationship between porosity and temperature complies with the power index function; the porosity of frozen sandstone and its uniaxial compressive strength meet the power index function relationship.

Published
2020
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6. Highly catalytic and stable Au@AuPt nanoparticles for visual and quantitative detection of E. coli O157:H7.

Author

Zhang, Yanyu, Su, Jiangshang, Fu, Tingting, Zhang, Wanzhen, Xiao, Yujuan, and Huang, Yishun

Subjects
ESCHERICHIA coli, ESCHERICHIA coli O157:H7, DRINKING water, NANOPARTICLES
Abstract

A visual and quantitative ELISA-like method for Escherichia coli O157:H7 is developed based on highly catalytic and stable Au@AuPt nanoparticles. The proposed enhanced ELISA method can visually detect 100 CFU mL−1 O157:H7 with high specificity and without the need for strict low-temperature reagent storage, thereby increasing the utility. Moreover, it is applicable to spiked tap water and milk tea samples without additional treatments. [ABSTRACT FROM AUTHOR]

Published
2023
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11. The single-tube quantitative rapid detection of coliform bacteria based on enzyme-specific technology

Author

Huang Yishun, Weiyun Zhang, and Jinzhong Cai

Subjects
0303 health sciences, Chromatography, Spectrometer, Bacteria, 030306 microbiology, Chemistry, media_common.quotation_subject, Metals and Alloys, Enzyme-Linked Immunosorbent Assay, General Chemistry, beta-Galactosidase, Rapid detection, Catalysis, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Coliform bacteria, Single tube, 03 medical and health sciences, Software portability, Materials Chemistry, Ceramics and Composites, Simplicity, 030304 developmental biology, media_common
Abstract

To detect coliform bacteria (CB) with simplicity and portability, a novel, single-tube quantitative rapid detection method was developed based on CB-specific enzymatic technology. This technology involved a simple multi-channel spectrometer which could detect 7 cfu mL−1 CB specifically, and the approach was applied to various food and water samples.

Published
2021

13. Study on Mechanical Behavior of Jurassic Frozen Sandstone in Western China Based on NMR Porosity

Author

Guangyong Cao, Huang Yishun, Lin Jian, Shiliang Xu, and Ma Maoyan

Subjects
Ground freezing, Article Subject, Chemistry, 0211 other engineering and technologies, 02 engineering and technology, General Chemistry, Overburden pressure, Residual strength, Brittleness, Cohesion (geology), Growth rate, Composite material, Porosity, QD1-999, Elastic modulus, 021102 mining & metallurgy, 021101 geological & geomatics engineering
Abstract

Study of frozen rock mechanical properties is necessary for safe application of the artificial ground freezing method in excavation of Chinese western water-rich soft rock layers. Triaxial compression tests and NMR test for samples from the western Jurassic sandstone were performed to investigate rock mechanical properties affected by low temperature and confining pressure. The results show mechanical parameters such as peak strength, cohesion, internal friction angle, residual strength, and elasticity modulus increased with the decreasing temperature under stable pressure, and the above parameters increased with the increasing confining pressure at a certain temperature. In particular, the growth rate of the rock strength would decline when the temperature was below −10°C in this study. Strength attenuation coefficients increased with the decreasing temperature, which indicated higher brittleness, whereas plastic characters got more obvious with the increasing confining pressure at a stable temperature. Furthermore, during the first two freezing stages, porosity decreased sharply with obvious increase of pore (crack) ice content, while porosity varied little at the third stage, which was the reason for the growth rate of rock strength declining with continuous low temperature from microcosmic point of view.

Published
2020
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38. Highly sensitive and selective detection of miRNA: DNase I-assisted target recycling using DNA probes protected by polydopamine nanospheres.

Author

Xie, Yi, Lin, Xiaoyan, Huang, Yishun, Pan, Rujun, Zhu, Zhi, Zhou, Leiji, and Yang, Chaoyong James

Subjects
DNA probes, NUCLEASES, DNA, MICRORNA, DEOXYRIBONUCLEOTIDES
Abstract

Based on the protective properties of polydopamine nanospheres for DNA probes against nuclease digestion, we have developed a DNase I-assisted target recycling signal amplification method for highly sensitive and selective detection of miRNA. [ABSTRACT FROM AUTHOR]

Published
2015
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39. DNA Hydrogel with Tunable pH-Responsive Properties Produced by Rolling Circle Amplification.

Author

Xu W, Huang Y, Zhao H, Li P, Liu G, Li J, Zhu C, and Tian L

Subjects
Circular Dichroism methods, Electrophoresis, Polyacrylamide Gel methods, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning methods, Nucleic Acid Amplification Techniques, Phase Transition, Rheology methods, DNA chemical synthesis, Hydrogels chemistry, Oligonucleotides chemistry
Abstract

Recently, smart DNA hydrogels, which are generally formed by the self-assembly of oligonucleotides or through the cross-linking of oligonucleotide-polymer hybrids, have attracted tremendous attention. However, the difficulties of fabricating DNA hydrogels limit their practical applications. We report herein a novel method for producing pH-responsive hydrogels by rolling circle amplification (RCA). In this method, pH-sensitive cross-linking sites were introduced into the polymeric DNA chains during DNA synthesis. As the DNA sequence can be precisely defined by its template, the properties of such hydrogels can be finely tuned in a very facile way through template design. We have investigated the process of hydrogel formation and pH-responsiveness to provide rationales for functional hydrogel design based on the RCA reaction., (© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2017
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40. Graphene oxide protected nucleic acid probes for bioanalysis and biomedicine.

Author

Cui L, Song Y, Ke G, Guan Z, Zhang H, Lin Y, Huang Y, Zhu Z, and Yang CJ

Subjects
Antineoplastic Agents administration & dosage, Antineoplastic Agents chemistry, Antineoplastic Agents toxicity, Apoptosis drug effects, Aptamers, Nucleotide chemistry, Doxorubicin administration & dosage, Doxorubicin chemistry, Doxorubicin toxicity, Drug Carriers chemistry, Gene Transfer Techniques, HeLa Cells, Humans, Inhibitor of Apoptosis Proteins genetics, Inhibitor of Apoptosis Proteins metabolism, Microscopy, Confocal, Nanotubes, Carbon chemistry, Neoplasms drug therapy, Oxides chemistry, RNA, Messenger analysis, RNA, Small Interfering administration & dosage, RNA, Small Interfering chemistry, Survivin, Graphite chemistry, Nucleic Acid Probes chemistry
Abstract

Recently, the binding ability of DNA on GO and resulting nuclease resistance have attracted increasing attention, leading to new applications both in vivo and in vitro. In vivo, nucleic acids absorbed on GO can be effectively protected from enzymatic degradation and biological interference in complicated samples, making it useful for targeted delivery, gene regulation, intracellular detection and imaging with high uptake efficiencies, high intracellular stability, and very low toxicity. In vitro, the adsorption of ssDNA on GO surface and desorption of dsDNA or well-folded ssDNA from GO surface result in the protection and deprotection of DNA from nucleic digestion, respectively, which has led to target-triggered cyclic enzymatic amplification methods (CEAM) for amplified detection of analytes with sensitivity 2-3 orders of magnitude higher than that of 1:1 binding strategies. This Concept article explores some of the latest developments in this field., (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2013
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