1. Selective Desorption of Intercalated Bovine Serum Albumin and Lysozyme by Organically Modified Montmorillonite
- Author
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Tsung-Yen Tsai, Ti-Chia Wu, Huang Sin-Hsin, Chao-Chen Hsu, Victor Wei‐Keh Chao, Chieh Chan, and Ping‐Han Wang
- Subjects
Gel electrophoresis ,Chromatography ,biology ,General Chemistry ,chemistry.chemical_compound ,Adsorption ,Montmorillonite ,chemistry ,Desorption ,biology.protein ,Sodium dodecyl sulfate ,Bovine serum albumin ,Lysozyme ,Selectivity - Abstract
The organically modified montmorillonite (M-Mt) was applied as an adsorbent for the purification of bovine serum albumin (BSA). In order to differentiate the selectivity and perform the purification of BSA, two kinds of proteins, BSA and lysozyme (LYZ) were mixed together and prepared at different pH, which could change the electrical charges on the surfaces of the proteins. BSA and LYZ can be adsorbed at the lower pH into the organically modified montmorillonite, which could be confirmed by powder X-ray diffraction (XRD) in the d-value increased after the adsorption of proteins. However, there is only BSA desorption was observed, approved by the method of Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE), from this adsorbed protein mixture when the pH of the solution was adjusted and optimized. These results indicate that there is electrostatic interaction between a suitably modified montmorillonite and proteins BSA and LYZ to perform the selective desorption from BSA in the mixture of these proteins.
- Published
- 2015