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21 results on '"Huang, Liangzong"'

12. Variations in NSP1 of Porcine Reproductive and Respiratory Syndrome Virus Isolated in China from 1996 to 2022.

Author

Zhang, Zhiqing, Zhang, Hang, Luo, Qin, Zheng, Yajie, Kong, Weili, Huang, Liangzong, and Zhao, Mengmeng

Subjects
PORCINE reproductive & respiratory syndrome, AMINO acid sequence, SIGNAL peptides, GENETIC variation, GENETIC distance
Abstract

Since its successful isolation in China in 1995, the porcine reproductive and respiratory syndrome virus (PRRSV) has been mutating into highly pathogenic strains by constantly changing pathogenicity and genetic makeup. In this study, we investigated the prevalence and genetic variation of nonstructural protein 1 (NSP1) in PRRSV-2, the main strain prevalent in China. After formulating hypotheses regarding the biology of the NSP1 protein, the nucleotide and amino acid similarity of NSP1 were analyzed and compared in 193 PRRSV-2 strains. The results showed that NSP1 has a stable hydrophobic protein with a molecular weight of 43,060.76 Da. Although NSP1 lacked signal peptides, it could regulate host cell signaling. Furthermore, NSP1 of different strains had high nucleotide (79.6–100%) and amino acid similarity (78.6–100%). In the amino acid sequence comparison of 15 representative strains of PRRSV-2, multiple amino acid substitution sites were found in NSP1. Phylogenetic tree analysis showed that lineages 1 and 8 had different evolutionary branches with long genetic distances. This study lays the foundation for an in-depth understanding of the nature and genetic variation of NSP1 and the development of a safe and effective vaccine in the future. [ABSTRACT FROM AUTHOR]

Published
2023
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19. Identification of an H6N6 swine influenza virus in southern China

Author

Zhang, Guihong, primary, Kong, Weili, additional, Qi, Wenbao, additional, Long, Li-Ping, additional, Cao, Zongxi, additional, Huang, Liangzong, additional, Qi, Haitao, additional, Cao, Nan, additional, Wang, Wenhua, additional, Zhao, Furong, additional, Ning, Zhangyong, additional, Liao, Ming, additional, and Wan, Xiu-Feng, additional

Published
2011
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20. High Prevalence of Plasmid-Mediated Quinolone Resistance Determinants qnr , aac(6 ′ ) - Ib-cr , and qepA among Ceftiofur-Resistant Enterobacteriaceae Isolates from Companion and Food-Producing Animals

Author

Ma, Junying, primary, Zeng, Zhenling, additional, Chen, Zhangliu, additional, Xu, Xiaogang, additional, Wang, Xiaoying, additional, Deng, Yuting, additional, Lü, Dianhong, additional, Huang, Liangzong, additional, Zhang, Yunyuan, additional, Liu, Jianhua, additional, and Wang, Minggui, additional

Published
2009
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21. High Prevalence of Plasmid-Mediated Quinolone Resistance Determinants qnr, aac(6′)-Ib-cr, and qepAamong Ceftiofur-Resistant EnterobacteriaceaeIsolates from Companion and Food-Producing Animals

Author

Ma, Junying, Zeng, Zhenling, Chen, Zhangliu, Xu, Xiaogang, Wang, Xiaoying, Deng, Yuting, Lü, Dianhong, Huang, Liangzong, Zhang, Yunyuan, Liu, Jianhua, and Wang, Minggui

Abstract

ABSTRACTThree kinds of plasmid-mediated quinolone resistance (PMQR) determinants have been discovered and have been shown to be widely distributed among clinical isolates: qnrgenes, aac(6′)-Ib-cr, and qepA. Few data on the prevalence of these determinants in strains from animals are available. The presence of PMQR genes in isolates from animals was determined by PCR amplification and DNA sequencing. The production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases in the strains was detected, and their genotypes were determined. The genetic environment of PMQR determinants in selected plasmids was analyzed. All samples of ceftiofur-resistant (MICs ≥ 8 μg/ml) isolates of the family Enterobacteriaceaewere selected from 36 companion animals and 65 food-producing animals in Guangdong Province, China, between November 2003 and April 2007, including 89 Escherichia coliisolates, 9 Klebsiella pneumoniaeisolates, and isolates of three other genera. A total of 68.3% (69/101) of the isolates produced ESBLs and/or AmpC β-lactamases, mainly those of the CTX-M and CMY types. Of the 101 strains, PMQR determinants were present in 35 (34.7%) isolates, with qnr, aac(6′)-Ib-cr, and qepAdetected alone or in combination in 8 (7.9%), 19 (18.8%), and 16 (15.8%) strains, respectively. The qnrgenes detected included one qnrB4gene, four qnrB6genes, and three qnrS1genes. Five strains were positive for both aac(6′)-Ib-crand qepA, while one strain was positive for qnrS1, aac(6′)-Ib-cr, and qepA. qnrB6was flanked by two copies of ISCR1with an intervening dfrgene downstream and sul1and qacEΔ1genes upstream. In another plasmid, aac(6′)-Ib-crfollowed intI1and arr-3was downstream. PMQR determinants are highly prevalent in ceftiofur-resistant Enterobacteriaceaestrains isolated from animals in China. This is the first report of the occurrence of PMQR determinants among isolates from companion animals.

Published
2009
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