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[Display omitted] • The TRP family channels of Sebastes schlegelii were identified by genome-wide identification and homologous species sequence comparison. • The Protein characterizations of TRP family of Sebastes schlegelii were analyzed. • The transcriptional levels of thermoTRP genes to high temperature was studied. Transient Receptor Potential (TRP) channels, essential for sensing environmental stimuli, are widely distributed. Among them, thermosensory TRP channels play a crucial role in temperature sensing and regulation. Sebastes schlegelii , a significant aquatic economic species, exhibits sensitivity to temperature across multiple aspects. In this study, we identified 18 SsTRP proteins using whole-genome scanning. Motif analysis revealed motif 2 in all TRP proteins, with conserved motifs in subfamilies. TRP-related domains, anchored repeats, and ion-transmembrane domains were found. Chromosome analysis showed 18 TRP genes on 11 chromosomes and a scaffold. Phylogenetics classified SsTRPs into four subfamilies: TRPM, TRPA, TRPV, and TRPC. In diverse organisms, four monophyletic subfamilies were identified. Additionally, we identified key TRP genes with significantly upregulated transcription levels under short-term (30 min) and long-term (3 days) exposure at 24 °C (optimal elevated temperature) and 27 °C (critical high temperature). We propose that genes upregulated at 30 min may be involved in the primary response process of temperature sensing, while genes upregulated at 3 days may participate in the secondary response process of temperature perception. This study lays the foundation for understanding the regulatory mechanisms of TRPs responses to environmental stimuli in S. schlegelii and other fishes. [ABSTRACT FROM AUTHOR]

Railway wheel polygonization is a type of uneven wear of material which worsens the running stability of trains. As a significant factor influencing the wear, wheel-rail hardness matching (i.e., the hardness ratio H W / H R) might affect the formation of a polygonal wheel. Thus, rolling-sliding wear tests were conducted using three wheel steels matching two rail steels to explore the influence of H W / H R (ranging from 0.649 to 1.263) on the initiation and evolution of wheel polygonal wear. The results show that when matching the softer rail, the wheel was less likely to become polygonal with an increase in H W / H R. When matching the harder rail, the wheel presented earlier polygon initiation under a moderate H W / H R of 0.792. The largest H W / H R of 1.263 presented the best anti-polygon condition for wheel material. The wear mechanism of polygonal wheel was dominated by abrasive, fatigue and oxidative wear at the crest and fatigue wear at the trough. • Evolution of wheel polygon wear is analyzed via rolling-sliding test with high-speed camera and vibration sensor. • Effect of wheel-rail hardness ratio on wheel polygon wear is explored. • Wheel polygon wear is easier to form with wheel hardness decreasing and rail hardness increasing. • Wear mechanism at crest is different with that at trough. [ABSTRACT FROM AUTHOR]

Background: Spinal muscular atrophy (SMA) is a rare neuromuscular disorder threating hundreds of thousands of lives worldwide. And the severity of SMA differs among different clinical types, which has been demonstrated to be modified by factors like SMN2, SERF1, NAIP, GTF2H2 and PLS3. However, the severities of many SMA cases, especially the cases within a family, often failed to be explained by these modifiers. Therefore, other modifiers are still waiting to be explored. Case presentation: In this study, we presented a rare case of SMA discordant family with a mild SMA male patient and a severe SMA female patient. The two SMA cases fulfilled the diagnostic criteria defined by the International SMA Consortium. With whole exome sequencing, we confirmed the heterozygous deletion of exon7 at SMN1 on the parents' genomes and the homozygous deletions on the two patients' genomes. The MLPA results confirmed the deletions and indicated that all the family members carry two copies of SMN2, SERF1, NAIP and GTF2H2. Further genomic analysis identified compound heterozygous mutations at TLL2 on the male patient's genome, and compound heterozygous mutations at VPS13A and the de novo mutation at AGAP5 on female patient's genome. TLL2 is an activator of myostatin, which negatively regulates the growth of skeletal muscle tissue. Mutation in TLL2 has been proved to increase muscular function in mice model. VPS13A encodes proteins that control the cycling of proteins through the trans-Golgi network to endosomes, lysosomes and the plasma membrane. And AGAP5 was reported to have GTPase activator activity. Conclusions: We reported a case of SMA discordant family and identified mutations at TLL2, VPS13A and AGAP5 on the patients' genomes. The mutations at TLL2 were predicted to be pathogenic and are likely to alleviate the severity of the male SMA patient. Our finding broadens the spectrum of genetic modifiers of SMA and will contribute to accurate counseling of SMA affected patients and families. [ABSTRACT FROM AUTHOR]

The long-term maintenance of low concentration of dissolved oxygen (DO) is an important condition for the enrichment of anammox bacteria (AnAOB) in the electrochemical anammox system (EAS). In this study, a new method to control DO by the combination of sodium sulfite with nitrogen stripping was attempted, and the influence of sodium sulfite concentration on the nitrogen removal performance was studied. The results show that under the optimal conditions (i.e., sodium sulfite concentration = 200 mg/L, nitrogen stripping time = 40–60 min, nitrogen flow = 350 mL/min), the DO in the water was controlled below 0.3 mg/L, which saves more than half of the time required for removing DO compared with the nitrogen stripping method (120–140 min). However, the total nitrogen removal rate (NRR) at the optimal current density of 0.10 mA/cm2 decreased gradually, and the NRR was 0.0091, 0.0061, 0.0026, and 0.0006 g N/[L∙d] at the sodium sulfite concentration of 0, 200, 400, and 600 mg/L, respectively. Simultaneously the specific anammox activity decreased from 0.0077 g N/[g VSS∙d] to 0.0032 g N/[g VSS∙d]. It is concluded that when the current density was 0.10 mA/cm2 and the concentration of sodium sulfite was 200 mg/L, the optimal operating conditions were both anammox activity and deoxidation. Therefore, the combination of sodium sulfite with nitrogen stripping has great application potential to control DO in the EAS. [ABSTRACT FROM AUTHOR]

In order to study the influence of third mediums on the formation and evolution behaviours of rail corrugation, two series of rolling-sliding tests were performed. Firstly, tests under the dry and different third mediums (oil, grease, water, friction modifier and windblown sand) conditions were conducted to study the influence of third mediums on the formation of rail corrugation. Secondly, rail rollers were first rolled under the dry condition until a stable rail corrugation was formed. Then, water and friction modifier were added into the wheel-rail interface to investigate the role of third mediums on the evolution behaviours of rail corrugation. The results indicated that third mediums had a significant influence on the wheel-rail damage and the formation and evolution of rail corrugation. Compared with the dry condition, both the mass losses and the damage of rail rollers were alleviated under third mediums conditions. The continuous application of third mediums at the wheel-rail interface could alleviate the formation of rail corrugation significantly. When non-solid third mediums existed at the wheel-rail interface (oil, grease, water and friction modifier), no corrugation was observed. In the dynamic windblown sand environment, the corrugation was obviously slighter than that under the dry contact condition. After corrugation was formed on the rail roller, the application of water and friction modifier could alleviate the amplitude of rail corrugation. Furthermore, with the increase in the number of cycles, the amplitude of rail corrugation first increased and then remained nearly stable. Under the dry contact condition, the crack length in the trough was larger than that in the crest. When liquid third mediums were applied, the cracks at the crest were ground away. However, some liquid would enter into the pre-existed crack in the trough, resulting in the severe rolling contact fatigue damage of rail. • The influence of different third mediums on the generation and evolution of rail corrugation was studied. • The third medium (water, oil, grease, FM) could alleviate the generation of rail corrugation. • The application of third mediums, especially water, would result in the reduction of the amplitude of rail corrugation, the transition from oxidative wear to fatigue wear in the crest and severe fatigue damage in the trough. • The influence mechanism of third mediums on the development of rail corrugation was proposed. [ABSTRACT FROM AUTHOR]

A rapid construction of the tricyclic scaffold of annotinine hydrate has been accomplished in only four steps from conveniently available starting materials. This short synthesis featured an efficient Lewis acid-catalyzed Diels–Alder reaction to construct the 4a, 8a- cis -hydroquinoline skeleton and a 5- exo -trig radical cyclization reaction to assemble the γ-butylrolactone motif. Image 1 [ABSTRACT FROM AUTHOR]

Matrix metalloproteinases (MMPs) are a cluster of enzymes that degrade the extracellular matrix (ECM) and some intracellular proteins; as such, they play an important role in tissue regeneration, infant growth, animal reproduction, and immunity. Most research into MMPs focuses mainly on their effects on the mammalian immune system. However, it is not clear how MMPs affect immune processes in crustaceans. Here, we cloned the open reading frame (ORF) of Eriocheir sinensis (Chinese mitten crab) MMP-14 (EsMMP-14) to explore the role of MMPs in crustacean innate immune responses. RT-PCR results showed that stimulation of crab with LPS and poly I:C upregulated expression of EsMMP-14 markedly. Besides, following the stimulation of 20-Hydroxyecdysone, the expression level of EsMMP-14 increased robustly, suggesting that EsMMP-14 involved in the molt process of E. sinensis. Hematoxylin and eosin staining of hepatopancreas and intestine revealed that knocking down EsMMP-14 maintained morphology following infection by Bacillus thuringiensis. Moreover, downregulated expression of EsMMP-14 increased the survival rate of infected E. sinensis. These results show that EsMMP-14 plays a role in innate immune responses of E. sinensis and fills a gap in our knowledge about the function of MMPs in crustaceans. • LPS and poly I:C stimulation induced overexpression of EsMMP-14. • EsMMP-14 expressed robustly following the stimulation of 20-Hydroxyecdysone. • RNAi of EsMMP-14 maintained normal tissue morphology following infection. • RNAi of EsMMP-14 increased the survival rate of infected E. sinensis. [ABSTRACT FROM AUTHOR]

The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins (aqps) in the testis is crucial. Turbot (Scophthalmus maximus) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4, 10, and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl2) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques related to sperm activation and cryopreservation, offering valuable knowledge for future advancements in this field. [ABSTRACT FROM AUTHOR]

Having a limited number of VH segments, cattle rely on uniquely long DH gene segments to generate CDRH3 length variation (3–70 aa) far greater than that in humans or mice. Bovine antibodies with ultralong CDRH3s (>50 aa) possess unusual structures and abilities to bind to special antigens. In this study, we replaced most murine endogenous DH segments with bovine DH genes, generating a mouse line termed B‐DH. The use of bovine DH genes significantly increased the length variation of CDRH3 and consequently the Ig heavy chain repertoire in B‐DH mice. However, no ultralong CDRH3 was observed in B‐DH mice, suggesting that other factors, in addition to long DH genes, are also involved in the formation of ultralong CDRH3. The B‐DH mice mounted a normal humoral immune response to various antigens, although the B‐cell developmental paradigm was obviously altered compared with wild‐type mice. Additionally, B‐DH mice are not predisposed to the generation of autoantibodies despite the interspecies DH gene replacement. The B‐DH mice reported in this study provide a unique model to answer basic questions regarding the synergistic evolution of DH and VH genes, VDJ recombination and BCR selection in B‐cell development. [ABSTRACT FROM AUTHOR]

In contrast to humans or rabbits, in which maternal IgG is transmitted to offspring prenatally via the placenta or the yolk sac, large domestic animals such as pigs, cows and sheep transmit IgG exclusively through colostrum feeding after delivery. The extremely high IgG content in colostrum is absorbed by newborns via the small intestine. Although it is widely accepted that the neonatal Fc receptor, FcRn, is the receptor mediating IgG transfer across both the placenta and small intestine, it remains unclear whether FcRn also mediates serum IgG transfer across the mammary barrier to colostrum/milk, especially in large domestic animals. In this study, using a FcRn knockout pig model generated with a CRISPR‐Cas9‐based approach, we clearly demonstrate that FcRn is not responsible for the IgG transfer from serum to colostrum in pigs, although like in other mammals, it is involved in IgG homeostasis and mediates IgG absorption in the small intestine of newborns. [ABSTRACT FROM AUTHOR]

Nemonoxacin is a novel non-fluorinated quinolone. The effect of nemonoxacin on modulation of host immune response is not known. We sought to determine whether nemonoxacin has immunoprotective effects on lipopolysaccharide (LPS)-induced mouse sepsis model. Therefore, mice were challenged with lethal dose LPS (12.5 mg/kg) only or LPS with multi-dose nemonoxacin (40 mg/kg q12h) by intraperitoneal injection, and the results showed nemonoxacin could significantly reduce mortality from 80 to 30% in this model. The effect of nemonoxacin on immune cells in vivo and in vitro was also investigated. Mice were treated with sublethal LPS (5 mg/kg) or LPS + nemonoxacin, the myeloid cell subsets in mouse spleen were analyzed by flow cytometry, and cytokines in mouse serum were measured by ELISA. Additionally, mouse macrophage RAW264.7 cells were treated with LPS or LPS + nemonoxacin to investigate the immune modulatory effect of nemonoxacin in vitro, and the level of cytokines in cell culture supernatant was determined by ELISA. Analysis of myeloid cell subsets in the spleen showed nemonoxacin pretreatment could significantly inhibit LPS-induced proliferation of macrophages and dendritic cells but have no effect on neutrophils. Nemonoxacin could significantly reduce the expression of pro-inflammatory cytokines IL-6 and TNF-α while increase anti-inflammatory cytokine IL-10 expression, which were induced by LPS in vivo and in vitro. Finally, the immunomodulation of nemonoxacin in macrophage phagocytosis was also examined. The results displayed nemonoxacin pretreatment could significantly enhance the phagocytic function of macrophage. In conclusion, nemonoxacin has immune modulatory and protective effect on LPS-induced inflammation in vivo and in vitro. [ABSTRACT FROM AUTHOR]

Background: To investigate the diagnostic efficacy of long noncoding RNA metastasis‐associated in lung adenocarcinoma transcript l (MALAT1) as a candidate serological biomarker for non‐small cell lung cancer (NSCLC). Methods: Diagnostic studies relevant to circulation long noncoding RNA MALAT1 as a candidate serological biomarker for NSCLC were electronically systematically searched in PubMed, EMBASE, EBSCO and CNKI databases. Suitable studies were included in the meta‐analysis by pooling the diagnostic sensitivity, specificity, positive likelihood ratio (+LR), negative likelihood ratio (−LR), diagnostic odds ratio (DOR) and area under the symmetric ROC curve (AUC) through a random or fixed effects model. Deeks' funnel plot was applied for publication bias evaluation. Results: Six studies with eight datasets were finally included in the meta‐analysis after a systematic search of the databases was performed. The pooled diagnostic sensitivity, specificity, +LR, −LR and DOR were 0.81 (95% CI:0.78–0.84), 0.67 (95% CI:0.63–0.71), 2.61 (95% CI:1.81–3.71), 0.28 (95% CI:0.19–0.43) and 13.73 (95% CI:6.19–30.44), respectively. The pooled area under the ROC curve (AUC) were 0.8663 and 0.8658, respectively by symmetric and asymmetric methods. Conclusion: Based on the results of our study, serum long noncoding RNA MALAT1 is a promising biomarker for NSCLC screening. However, due to its low specificity, MALAT1 positive cases need further validation for NSCLC by other diagnostic methods such as radiology, cytology, etc. [ABSTRACT FROM AUTHOR]

Chinese mitten crab (Eriocheir sinensis) is an economically important aquaculture species, and its growth and development are regulated by temperature, but the molecular mechanisms of the responses to temperature remain unclear. Herein, we identified TRPA1 from E. sinensis , a member of the TRP family of heat receptor potential channels, performed RACE cloning and bioinformatics analysis, and investigated the effect of TRPA1 on temperature responses and molting by real-time PCR and RNA interference (RNAi). The open reading frame of Es-TRPA1 is 3660 bp, and the encoded protein has a molecular weight of 136.91 kDa, and is expressed in embryos and juveniles. RNAi-mediated silencing decreased Es - TRPA1 expression in juvenile crabs, molting rate was decreased, mortality was increased, and crabs avoided cold areas (4 °C) much less than control juvenile crabs. The results suggest that Es-TRPA1 is involved in regulating temperature adaptation and molting processes in E. sinensis. The findings lay a foundation for further exploration of temperature regulation mechanisms in E. sinensis and other crustaceans. [Display omitted] • Es-TRPA1 is expressed in Eriocheir sinensis embryos and juveniles. • RNAi-mediated silencing decreased Es - TRPA1 expression in juvenile crabs. • Molting was decreased but mortality was increased in RNAi-silenced crabs. • RNAi-silenced crabs avoided cold areas less than control crabs. • ES-TRPA1 is involved in regulating temperature adaptation and molting. [ABSTRACT FROM AUTHOR]

Tigecycline resistance is emerging among Klebsiella pneumoniae , but knowledge regarding in vivo development of tigecycline resistance is limited. Here we report a new mechanism of tigecycline resistance in K. pneumoniae that evolved during tigecycline therapy. Klebsiella pneumoniae isolates were consecutively obtained from urine samples of a patient with scrotal abscess and urinary tract infection before and during tigecycline treatment. Two tigecycline-resistant K. pneumoniae strains (KP-3R and KP-4R; MIC = 8 µg/mL) were isolated after 41 days and 47 days of tigecycline therapy. These isolates had the same sequence type (ST11) and PFGE patterns as tigecycline-susceptible strains (KP-1S and KP-2S; MIC = 2 µg/mL) initially isolated from the patient. Compared with KP-1S and KP-2S, KP-3R and KP-4R exhibited higher expression of efflux pump AcrAB . Sequence comparison of the repressor gene ramR did not find any mutation within the open-reading frame that exist frequently in tigecycline-resistant K. pneumoniae . Instead, a 12-bp deletion of ramR upstream region including the ribosomal binding site (RBS) TGAGG was observed in KP-3R and KP-4R. qRT-PCR and immunoblotting analyses showed that KP-3R and KP-4R did not have impaired ramR transcription but had abolished RamR protein production. Furthermore, xylE reporter assay demonstrated that KP-3R and KP-4R had a defect in RamR translation caused by the 12-bp deletion. Complementing KP-3R and KP-4R with functional ramR suppressed expression of acrAB and consequently restored tigecycline susceptibility. This is the first report identifying deletion of the ramR RBS as a mechanism of in vivo tigecycline resistance in K. pneumoniae developing during tigecycline therapy. [ABSTRACT FROM AUTHOR]

Background: The occurrence of non-contiguous, multiple, and remote involvement tuberculous spondylitis is rare. The clinical presentation in patients with multifocal musculoskeletal tuberculosis may closely mimic that in patients with multiple bone metastases, which makes the accurate clinical diagnosis challenging. Herein, we report a multifocal musculoskeletal tuberculosis case that was misdiagnosed for 8 months as multiple bone metastases.Case Presentation: A 63-year-old male farmer of Chinese Han ethnicity presented to us with pain in left side of the neck, right side of the chest and the back for 10 months without typical tuberculosis symptoms. His past medical history, the CT and fluoroscopy-guided biopsy were negative for tuberculosis. Interferon gamma by T-SPOT was also negative. Radiological findings including CT, MRI and PET-CT suggested that the patient had multiple metastases. Accordingly, the patient was misdiagnosed as having musculoskeletal tumors until a swelling under the right nipple ulcerated. The smear test for acid-fast bacilli and the PCR test for TB-DNA of the pus from the swollen area were both positive, leading to the final correct diagnosis of musculoskeletal tuberculosis.Conclusion: The proper diagnosis of musculoskeletal tuberculosis is clinically challenging due to Mycobacterium tuberculosis variants involved and atypical presentations, especially when the lesions are multiple. Our findings indicate that multiple tuberculous spondylitis must be considered in the differential diagnosis of multiple musculoskeletal lesions. [ABSTRACT FROM AUTHOR]

Abstract: Background: The optimal methods for glucose control and reduction in the risk of cardiovascular disease are controversial. Findings from recent clinical trials have shown different viewpoints on the advantages and disadvantages of intensive lowering of glucose. We used echocardiography to explore the influence of the glucose-lowering rate on left ventricular function in patients with type 2 diabetes mellitus (T2DM). We also attempted to discover an effective glucose-lowering rate for patients with type 2 diabetes mellitus and coronary heart disease (T2DM-CHD). Methods: A total of 132 cases of T2DM and 135 cases of T2DM-CHD received intensive glucose therapy. After measuring left ventricular ejection fraction (LVEF) and the E/A ratio, variations and correlation factors were evaluated. Results: LVEF was significantly higher than before intensive therapy in the T2DM group with a glucose-lowering rate of ≤6mmol·L–1·d–1 (P <.05). LVEF was significantly lower than before intensive therapy in the T2DM-CHD group with a glucose-lowering rate >4mmol·L–1·d–1 (P <.05), whereas at the end of follow-up (3months), LVEF increased and no significant difference was observed between subgroups (P >.05). The E/A ratio increased among all subgroups after intensive therapy (P <.05). The waist–hip ratio, duration of T2DM, and age had a linear regression relationship with variations in LVEF before and after intensive therapy. Conclusions: For patients with T2DM and CHD, an excessively fast glucose-lowering rate could impair left ventricular systolic function. Long-term, good control of blood glucose could restore the impaired left ventricular systolic function caused by an excessively fast glucose-lowering rate. After intensive therapy, left ventricular diastolic function improved among all subgroups regardless of the glucose-lowering rate and CHD. [Copyright &y& Elsevier]

Abstract: In this study, the antimicrobial susceptibilities and prevalence of plasmid-mediated quinolone resistance determinants amongst Salmonella enterica serotype Typhimurium isolates from hospitalised paediatric patients with diarrhoea in China were investigated. In total, 40 (64.5%) of 62 S. Typhimurium isolates were resistant to ciprofloxacin (minimum inhibitory concentration ≥0.5μg/mL), comprising 28 isolates with low-level resistance and 12 isolates with high-level resistance. All ciprofloxacin-resistant isolates were multiresistant to other antimicrobial agents. Four pulsed-field gel electrophoresis (PFGE) clusters were found amongst the 40 ciprofloxacin-resistant isolates, amongst which PFGE clusters A, B, E and D accounted for 7, 4, 1 and 28 isolates, respectively. Two isolates with high-level ciprofloxacin resistance had two mutations in the quinolone resistance-determining regions (QRDRs) of gyrA and parC. The remaining ciprofloxacin-resistant isolates had only one mutation in the QRDR of gyrA. All 62 S. Typhimurium isolates were negative for qnr genes and qepA and 23 (37.1%) of the isolates were positive for aac(6′)-Ib-cr. Nineteen isolates harbouring aac(6′)-Ib-cr belonged to PFGE cluster D. A high prevalence of ciprofloxacin resistance and aac(6′)-Ib-cr was found amongst S. Typhimurium isolates in China from hospitalised paediatric patients with diarrhoea not receiving quinolones. A single mutation in the QRDR of gyrA as well as production of AAC(6′)-Ib-cr contributed to ciprofloxacin resistance. Clonal spread was responsible for the dissemination of aac(6′)-Ib-cr amongst S. Typhimurium isolates. [ABSTRACT FROM AUTHOR]

Gas–liquid two-phase flow is a typical flow. Its bubble characteristic measurement is of great importance on studying the flow mechanism and guiding the practical fluid mechanical engineering. In this paper, a novel three dimensional (3D) multiphase flow imaging device was designed and optimized to measure the transparent object that has an opaque object in the center of the 3D observed area. Its mathematical model was built and the constraints were defined based on the geometrical relationship and design requirements. Based on the original harmony search (HS) algorithm, a modified harmony search algorithm (MHS-MC) for improving both the performance on the global optima of the multi-modal problems and the convergence performance of the constrained optimization problems was integrated and applied to optimize the arrangement of the single-camera-multi-mirror device. As a case study, the 3D multiphase flow imaging method was applied in the 3D reconstruction of the cavitation bubble cluster inside a water hydraulic valve. The statistics of the Pareto data show the good performance of the MHS-MC algorithm. And the cavitation experimental results testify the effectiveness of the proposed MHS-MC algorithm. The cavitation bubble cluster can be reconstructed with quite high precision. [ABSTRACT FROM AUTHOR]

The article offers information on a study concerning the prevalence of mobilized colistin resistance (mcr-1)-producing Klebsiella pneumoniae in bloodstream infections in China. Topics discussed include determination of minimum inhibitory concentration (MIC) of MCR-1 positive isolates using the broth microdilution method; use of filter-mating method for conjugation experiments Escherichia coli J53 as the recipient strain; and mentions that whole-genome sequencing (WGS) was performed.