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Objective: To investigate the protective effect of the Yiqi Mingmu solution irrigated into rat on retinal injury of rat induced by ischemia reperfusion., Methods: SD rats were divided into four groups randomly: negative control group, model control group, low and high dose group. Increasing the intraocular pressure with anterior chambers infused liquid induced the models of retinal ischemia reperfusion of rats. Different doses of Yiqi Mingmu solution were respectively irrigated into rats 7 days before ischemia in low and high dose group. Normal salire instead was used in normal and model control group. At 1, 3, 7, 15 days after the ischemia reperfusion injury, the content of MDA and activity of SOD in rat's retina were measured. The histopathologic changes were observed with transmission electron microscopy and light microscopy., Results: At 3, 7, 15 days after the ischemia reperfusion injury, the activity of SOD in Yiqi Mingmu solution low and high dose group were higher than in the model control group, the content of MDA in Yiqi Mingmu solution high dose group were lower than in the model control group. The histopathologic injury of retina in the Yiqi Mingmu solution low and high dose group were less than in the model control group., Conclusion: Yiqi Mingmu solution has protective effect on rat's retina ischemia reperfusion injury, and it is an effective traditional Chinese recipe to treat the diseases of retina ischemia.

Objective: To study the cytotoxic effects of three kinds of eye drops on human cancer cells and normal cells., Methods: Cytotoxic effects of Yuxingcao Eye Drop (YXC), Gegensu Eye Drop (GGS) and Shuanghuanglian Eye Drop (SHL) on 6 cell lines (CNE2, Glc-82, HRPE, Fibro, 3T3 and ECV-304) were assayed using MTF method. And cytotoxic efficacy was evaluated using fifty percent of inhibitory concentration (IC50)., Results: IC50 of YXC to human cancer cells CNE2 and Glc-82 were (11.5 +/- 0.25) microl/ml and (24.0 +/- 0.8) microl/ml; to normal cells HRPE, Fibro, 3T3 and ECV-304 were (18.0 +/- 3.5) microl/ml, (52.0 +/- 14.0) microl/ml, (17.5 +/- 3.5) microl/ml and (17.5 +/- 1.3) microl/ml, respectively. IC50 of GGS to CNE2, Glc-82, ECV-304 cells were (9.8 +/- 2.3) microl/ml, (17.0 +/- 5.0) microl/ml and (10.5 +/- 0.95) microl/ml, respectively. Under the concentration of 100 microl/ml, the average survival rates of HRPE, Fibro and 3T3 cells were 60.0%, 87.8% and 58.2%, respectively. IC50 of SHL to CNE2, Glc-82 and HRPE cells were (18.9 +/- 5.0) microl/ ml, (23.9 +/- 0.6) micorl/ml and (113.9 +/- 25.6) microl/ml. At the concentration of 100 microl/ml, the average survival rates of Fibro, 3T3 and ECV-304 Cells were 89.6%, 77.2% and 74.7%., Conclusion: YXC has evident cytotoxicities to human cancer cells and normal cells. While, GGS and SHL has not obvious cytotoxic effects to normal cells.