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This article first proposes the design of the ZigBee wireless sensor network node based on Arduino, from the design and implementation of the node hardware system to the design and implementation of the node software system, and expands on the design details. The node function was applied to the node design environment, and simulation tests were performed, and execution was tested to confirm the feasibility and practicability of the design. Based on this, the article proposes the service construction process of the software and hardware platform of the smart supply chain system, and then clarifies the basic mathematical problem of the distribution plan in line with the service strategy, that is, “the logistics route optimization algorithm with capacity and time constraint optimization,” which aims to study statically design, define, and model, and test two precise solutions representing heuristic genetic algorithms and analyze their results. In addition, additional hardware such as smart supply chain and cabinet boards are tested and designed according to system requirements, and the interaction mechanism is adapted to the user interface and supporting structure design so as to ensure the completion of user-related business processes. Combining the existing theoretical knowledge, the first priority is to meet customer needs, build a smart supply chain model under the premise of on-time delivery, and solve the model in time. Based on this point, this article tries to use hybrid branch algorithm link and tabu search algorithm to increase the consideration of traffic conditions, collect accurate algorithms, and modern heuristic algorithms to solve the actual situation of the case. At the end, the article proposes some related issues for the above problems. Suggesting through the research of wireless sensor network, this paper applies it to the research of intelligent supply chain and logistics route optimization algorithm and promotes the development of more intelligent supply chain in the future.

Sr3Al2-xBxO5Cl2:Eu2+, Dy3+ (x = 0, 0.2, 0.4) long persistent phosphors were prepared via solid-state process. The pristine Sr3Al2O5Cl2:Eu2+, Dy3+ phosphor exhibits orange/red broad band emission around 609 nm, which can be attributed to the electric radiation transitions 4f65 d1→4f7 of Eu2+. Upon the same excitation, the B3+-doped Sr3Al2-xBxO5Cl2:Eu2+, Dy3+ phosphors display red-shift from 609 nm to 625 nm with increasing B3+ concentrations. The XRD patterns show that Al3+ can be replaced by B3+ in the host lattice at the tetrahedral site, which causes lattice contraction and crystal field enhancement, and thereafter achieves the red-shift on the emission spectrum. The XPS investigation provides direct evidence of the dominant 2-valent europium in the phosphor, which can be ascribed for the broad band emission of the prepared phosphors. The afterglow of all phosphors show standard double exponential decay behavior, and the afterglow of Sr3Al2O5Cl2:Eu2+, Dy3+is rather weak, while the sample co-doped with B3+shows longer and stronger afterglow, as confirmed after the curve simulation. The analysis of thermally stimulated luminescence showed that, when B3+ is introduced, a much deeper trap is created, and the density of the electron trap is also significantly increased. As a result, B3+ ions caused redshift and enhanced afterglow for the Sr3Al2-xBxO5Cl2:Eu2+, Dy3+ phosphor.

Rice is the important crop for more than half of the world population. However, drought can have a devastating impact on rice growth and reduce yield drastically. Understanding the response of rice to drought stress is especially important for crop breeding. Previously we found that rice enhanced its tolerance to drought stresses via stress memory mechanisms. Numerous memory genes were identified to play important roles in the process. DNA methylation was reported to mediate tolerance via regulating gene expression and enhances the survival rate of rice encountering drought stress. However, how DNA methylation involved in stress memory is still not clear. In this study, genome-wide bisulphite sequencing at a single base resolution methylome profiling level was performed and analyzed in rice cultivar under recurrent drought stresses and recovery treatments. We found that rice drought stress memory-related differentially methylated regions (DMRs) showed dynamic and distinct patterns. The drought-memory DMRs may regulate Transposable elements and gene expression to cope with short-term repeated drought stresses. Our findings of drought-memory DMRs can explain mechanisms of rice drought stress memory in a new perspective on global methylome details. Using epigenetic markers to breed drought-resistant rice would become a feasible way in the future research.

Carbon fiber (CF) as a multifunctional material with superb performance in aviation industry has serious drawback such as impedance mismatch which restricted the capability of using it as microwave absorber materials. In this research, a novel hierarchical carbon fiber@La0.7Sr0.3MnO@NiO (CF/LS/N) composite was facilely and successfully synthesized via sol-gel and subsequent hydrothermal reactions. The structural, morphological, magnetic and electromagnetic behavior of the composite were precisely evaluated via XRD, FESEM, XPS, VSM and VNA analysis. According to the systematic evaluation results, the synergistic positive effect of adding NiO nanoparticles is revealed in improving the microwave absorption performance of CF@La0.7Sr0.3MnO composite. Literally, dielectric features of the prepared composite enhanced by introducing NiO nanoparticles. Due to improvement in impedance matching, dipole polarization, multiple reflection/scattering, eddy current, interfacial polarization and magnetic resonance phenomenon the minimal reflection loss of the obtained hybrid composite reach to -26 dB with 2.6 mm thickness at filler content of 20 wt%. CF/LS/N composite is predictable to be suitable candidate for military purposes because of the lightweight, low cost and outstanding absorbing properties.

Key message QTLs for cold tolerance at the early seedling and booting stages were identified and qCTB1 was delimited to a 342-kb interval, and the breeding potential of QTL pyramiding was assessed. Abstract Cold stress is one of the main abiotic stresses that affects rice growth and production worldwide. Dissection of the genetic basis is important for genetic improvement of cold tolerance in rice. In this study, a new source of cold-tolerant accession from the Yunnan plateau, Lijiangxiaoheigu, was used as the donor parent and crossed with a cold-sensitive cultivar Deyou17, to develop 130 recombinant inbred lines (RILs) for quantitative trait locus (QTL) analysis. In total, three QTLs for cold tolerance at the early seedling stage on chromosomes 2 and 7, and four QTLs at the booting stage on chromosomes 1, 3, 5, and 7 were identified. Under the cold stress conditions in deep water and in a high-altitude area, qCTB1 , a locus for cold tolerance at the booting stage, was fine mapped to a 342-kb interval between markers M3 and M4. Effect assessment in the RIL and BC 3 F 3 populations demonstrated that qCTB1 showed a stable effect in the genetic segregation populations. Haplotype and linear regression analyses showed that QTL pyramiding based on the additive effect of favorable loci possesses good potential for cold tolerance breeding. This study will provide genetic resources for molecular breeding and gene cloning of cold tolerance in rice.

Traditional breeding strategies mainly focus on the evaluation of trait performance, but pay less attention to the changing genetic background. A comprehensive understanding of the genetic diversity of germplasms is crucial for the deliberate improvement of specific traits. A collection of 154 highland rice varieties were collected as the initial genetic resource in our breeding program to improve the pathogen resistance and eating and cooking qualities. These varieties were analyzed using a whole-genome SNP array and were clustered into three groups. Further analysis revealed that the favorable alleles of pathogen resistance genes are mostly absent in our collected varieties. However, it showed that most varieties possess favorable alleles of Waxy (Wx) and ALKALI DEGENERATION (ALK), which are able to enhance the eating and cooking qualities. Moreover, only about one fifth of all varieties harbors favorable the allele of fragrance gene Betainealdehyde dehydrogenase (BADH2). Together, these results give an overall view of the genetic constitution of the target traits, which provide useful information for future genetic improvement in breeding practices.

According to the research on reservoir water resources scheduling and distribution, the aim is to balance the water supply and demand in each period, and consider the total water supply and the annual external water withdrawal of the reservoir in each period as water rights. The decision-making variables are provided for the water supply of the reservoir in this paper, so that water demand of the water-receiving area can be better met to alleviate the water shortage at various stages and realize the effective use of water resources. Moreover, through the constraints of reservoir operation rules and other constraints, a mathematical model is established for optimal operation of water resources in the reservoir system. Meanwhile, optimized genetic algorithms are applied to solve the model according to the characteristics of the model. After simulation tests, compared with the traditional linear binary algorithm used in the reservoir, the improved genetic algorithm studied in the paper improves the accuracy of data calculation and data convergence, which proves that the research results of the paper provide theoretical and practical significance for improving the level of reservoir water resources management and solving the problem of optimal water resources scheduling. HIGHLIGHTS This paper realizes the supply decision variables.; The data index takes the historical data of the past as a reference to compare the algorithm.; The solution model of this algorithm makes use of the genetic effect of the improved genetic algorithm.; The constraint conditions are used as the rules of data allocation.; A multi-group genetic algorithm based on real coding is proposed.

Chemotherapy resistance is one of the main reasons for tumor-related death. In particular, ovarian cancer patients often acquire drug resistance after chemotherapy. In this study, we found that the histone chaperone, nucleosome assembly protein 1-like 3 (NAP1L3), was significantly upregulated in tissues with cisplatin resistance compared with cisplatin-sensitive tissues. Patients with high NAP1L3 levels had poor prognosis, suggesting that NAP1L3 might regulate ovarian cancer resistance. Colony formation and terminal deoxynulceotidyl transferase nick-end-labeling (TUNEL) assays showed cells with high NAP1L3 had high cisplatin resistance, whereas cells with low NAP1L3 had poor cisplatin resistance. NAP1L3 overexpression significantly increased cisplatin resistance, whereas NAP1L3 knockdown significantly reduced cisplatin resistance, suggesting that NAP1L3 promoted cisplatin resistance. Mechanistically, gene set enrichment analysis and luciferase reporter assays showed that NAP1L3 regulated the transforming growth factor-beta (TGF-β) pathway. NAP1L3 overexpression increased the phosphorylation and nuclear translocation of SMAD family member 2 (SMAD2) and SMAD3, confirming that NAP1L3 activated the TGF-β pathway. Therefore, NAP1L3 might represent a novel target to overcome ovarian cancer chemoresistance.

Objective Laryngeal cancer is a common malignant tumor in the ENT, of which laryngeal squamous cell carcinoma (LSCC) accounts for more than 90% of laryngeal cancer. The purpose of this study is to investigate the regulatory mechanism of lncRNA SNHG16 in LSCC. Materials and methods Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to measure mRNA expression. Cell Counting Kit (CCK-8), Transwell and luciferase reporter assays, flow cytometric analysis and Western blot analysis were used to investigate the function of lncRNA SNHG16 in LSCC. Results SNHG16 expression was increased in LSCC tissues and cells. The abnormal expression of SNHG16 was associated with clinical stage and lymph node metastasis in LSCC patients. In addition, knockdown of SNHG16 restrained cell proliferation, migration and invasion in LSCC. More importantly, SNHG16 acted as a competitive endogenous RNA in LSCC and regulated FOXP4 expression by making miR-877-5p sponge. Further, SNHG16 promoted LSCC progression by interacting with miR-877-5p and FOXP4. Conclusion LncRNA SNHG16 promotes the progression of LSCC by sponging miR-877-5p and upregulating FOXP4.

In recent years, construction of large transportation hubs in various countries around the world has been in full swing, among which China’s large transportation hubs like those for high-speed trains, airports, and their composite buildings have been developing rapidly. The constant technological innovations of the Chinese high-speed train industry have led to the erection of one after another iconic high-speed train stations, further driving the development of the construction and design of large transportation hubs. Countries across the globe all have relevant standards for fire safety design, which is an important subproject of architecture design as it is directly related to people’s life and property safety, such as the NFPA and China’s Code for Fire Safety Design of Buildings. However, for large transportation hub buildings with great height, large space, and relatively complex functions, the fire safety design often cannot completely cover the existing standard codes. Therefore, such buildings normally adopt the performance-based design method to customize fire safety strategies. This book uses China’s large transportation hubs as an example to systematically summarize the fire safety design of large transportation hub buildings.

The US standards on fire safety design for transportation hubs are mainly based on the NFPA 130. The history of this standard will be briefly described in this section [9].

As the largest modern transportation hub project in the Yangtze River Delta constructed in Shanghai, Hongqiao Integrated Transportation Hub integrates various transportation modes including air, railway, highway passenger transportation, and public traffic transfer. After completion, the hub will connect the three metropolitan circles of Shanghai, Nanjing, and Hangzhou as well as economically developed areas like Suzhou, Wuxi, and Changzhou, covering a development network with Shanghai as the central economic city.

BIM (Building Information Modeling) technology means creating a reusable 3D digital model that integrates project entities and functions. The information of the building life cycle including design, construction, and operation is integrated on the same platform to realize intuitive expression, collaborative operation, information sharing, and data exchange. With this technology, it is possible to predict various problems that will occur in the later construction and operation phases in the early design stage, integrate and deal with hidden dangers in advance, reduce unnecessary resource consumption, and improve management efficiency and safety in the project life cycle. After successful trials, the technology has received extensive attention and been well applied in the engineering field. Large transportation hubs feature crowded people, high fire safety levels, complete fire control systems, and high degree of coordination among systems, and pipelines should be arranged comprehensively and reasonably without collision. These features cause difficulties for fire safety design, construction, and later operation, maintenance, and management, and these problems can be rightly solved via BIM technology [14].

Take Xi’ An Xianyang International Airport, for example. The main building of T3A is a very vast ticket hall, and the 327 × 108 m rectangle is supported by 40 columns made with concrete-filled steel tubes, while the fork-shaped steel column supports 20 steel tube trusses above the 35,000 sqm comprehensive hall. The check-in counters in the departure hall abandon the old “room within a room” model, which improves the transparency of the whole comprehensive hall.

The first part of this book summarizes the development of the world’s large transportation hubs and the evolution of their architectural forms and concludes the transformation of the function of transportation hubs to transfer center of a city’s internal and external traffic, the development trend from waiting to passing, the development trend of building space from plane functional space to three-dimensional functional space, the transformation from single-function space to compound space, and the shift from indirect transfer to zero-distance transfer. Due to the superposition of traffic and commercial service functions, this compound space has the characteristics of large plane area and high space. The difficult point in fire prevention design is that there are over-specification situations in fire compartment, smoke control and evacuation, etc., which is also the focus of this paper.

The interaction as well as the adsorption behavior of an (HF)n linear chain for n = 1–4 was inspected on an AlN nanocage using ab initio calculations to understand the ability of this material to monitor and sense the (HF)n in gas and water phases. The adsorption energies, optimized configurations, charge transfer, and the work function for this gas were calculated on the AlN nanocage surface. Also, the effect of the HF linear chain size was investigated on the adsorption energy and all of the electronic properties. According to the adsorption energy, the most stable configuration for the (HF)n on the AlN nanocage surface is the dimer form of the HF gas. Also, after the adsorption of the (HF)n on the AlN nanocage, the HOMO-LUMO gap decreased dramatically from 3.99 to 3.36 eV, leading to an increase in conductivity. Furthermore, the AlN nanocage can sense the (HF)n in the presence of many environmental pollutants.

Background: Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma (NPC), but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs (miRNAs) in NPC cells. Methods: Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis. Results: A total of 14 miRNAs exhibited >2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs. Conclusion: EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.

Fault line detection timely and accurately when single-phase-to-earth fault occurs in resonant grounding system is paid attention by many researchers. A new approach for fault detection based on data fusion which has non-artificial setting is presented in this paper. First, the fault criterion for interphase difference energy ratio and time-frequency correlation coefficient of each line is proposed. Then, a coordinate system with the interphase difference energy ratio as X axis and the time-frequency correlation coefficient as Y axis is established, and it uses the Euclidean distance algorithm to get the characteristic distance of each line by fusing two-dimensional information. Eventually, the sound distance and the fault distance of each line is compared to discriminate the fault line. The results and adaptability analysis from Electro-Magnetic Transients Program (EMTP) simulation demonstrate the effectiveness and reliability of the proposed scheme.

The Riemann solutions for the relativistic Euler equations for generalized Chaplygin gas are considered. It is rigorously proved that, as the pressure vanishes, they tend to the two kinds of Riemann solutions to the zero-pressure relativistic Euler equations, which include a delta shock formed by a weighted $\delta$-measure and a vacuum state.

Previous studies have shown that microRNA (miR)-610 is crucial in a variety of biological processes in various types of human cancer cells. However, the role of this microRNA in glioblastoma (GBM) is presently unclear. In this study, the role of miR-610 in cell proliferation was investigated in GBM. It was demonstrated that miR-610 expression is markedly downregulated in GBM cells and GBM tissues compared with normal human astrocytes (NHAs) and normal brain tissue, respectively. Ectopic expression of miR-610 reduced the proliferation and anchorage-independent growth of GBM cells, whereas inhibition of miR-610 promoted this effect. Bioinformatics analysis further revealed cyclin D2 (CCND2) and AKT3, putative tumor promoters, as potential targets of miR-610. Data from reporter assays showed that miR-610 directly binds to the 3′-untranslated region of CCND2 and AKT3 mRNA, and represses their expression at the transcriptional and translational levels. In conclusion, the data provide compelling evidence that miR-610 functions as an anti-onco-miRNA, which is important in inhibiting cell proliferation in GBM, and its anti-oncogenic effects are mediated chiefly through direct suppression of CCND2 and AKT3 expression.

Some existing fusion discriminations are used to identify whether a stator single-phase grounding fault occurs in parallel Powerformers. However, such methods are complicated to operate and require a large amount of training data as support. Therefore, a new discrimination of stator single-phase grounding fault based on multi-dimensional fusion for Powerformer is proposed. Firstly, the faulty Manhattan distance and the sound Manhattan distance about each Powerformer are calculated by the fusion formula of Manhattan distance base on the four features extracted in Powerformer. Then, the sizes of the faulty and sound Manhattan distances about each Powerformer are compared. If the faulty Manhattan distance of the Powerformer is less than the sound Manhattan distance, the Powerformer can be judged as the fault, otherwise, it can be judged as the sound Powerformer. The simulation experiment show that this method does not need a large number of data as support, nor does need to set the threshold value artificially, so it improves the practicability and applicability of the method.

Aberrant activation of the extracellular signal‑regulated kinases (ERKs)/ribosomal S6 kinase 2 (RSK2) signaling pathway is frequently determined in various human tumor types, including liver cancer, and has been considered as a promising target for cancer chemoprevention and therapy. In the present study, using computer‑aided virtual screening and molecular docking, isobavachalcone (IBC), a natural chalcone compound, was identified to be an ATP‑competitive inhibitor targeting ERK1/2 and RSK2. Cell Counting Kit‑8, EdU incorporation and colony formation assays were used to detect the effects of IBC on cell viability and proliferation, and the results demonstrated that IBC effectively inhibited the proliferation of liver cancer HepG2 and Hep3B cells, whereas it had no notable cytotoxic effect on immortal liver L02 cells. Flow cytometric analysis and western blotting further revealed that IBC caused significant levels of apoptosis on liver cancer cells via the caspase‑dependent mitochondria pathway. The computer prediction was confirmed with pull‑down and in vitro kinase assays, in which IBC directly bound with ERK1/2 and RSK2, and dose‑dependently blocked RSK2 kinase activity in liver cancer cells. Treatment of HepG2 or Hep3B cells with IBC significantly attenuated epidermal growth factor‑induced phosphorylation of RSK2 and resulted in the reduced activation of its downstream substrates including cAMP response element‑binding protein, activating transcription factor 1, histone H3 and activating protein‑1. Enforced RSK2 expression in L02 cells could increase the effect of IBC on suppressing cell growth. Conversely, knockdown of RSK2 reduced the inhibitory effect of IBC on HepG2 cell proliferation. Overall, the present data indicated that ERKs/RSK2 signaling serves a pivotal role in IBC‑induced suppression of liver cancer cells and that IBC may be a potential therapeutic candidate for human cancer with elevated ERKs/RSK2 activity.

Colorectal cancer (CRC) is the third most common cancer and the fourth leading cause of cancer-associated mortalities worldwide. MicroRNAs (miRNAs/miRs) serve important roles in tumor development, progression and metastasis. miR-221 has been reported to modulate proliferation, apoptosis, cell cycle distribution and cell migration in a variety of cancers. However, the function of miR-221 in the autophagy of cancer is unclear. In the present study, the role of miR-221 in the autophagy of CRC cells was investigated and its associated target was identified. Survival analysis using The Cancer Genome Atlas data suggested that a higher expression of miR-221 was associated with poor survival in patients with CRC. A Cell Counting kit-8 assay revealed that miR-221 promoted CRC cell proliferation. Autophagy flux analyzed by microtubule-associated protein 1 light chain 3 (LC3) turnover indicated that miR-221 reduced autophagy in CRC cells using different protease inhibitors (E64d and pepstatin A; Bafilomycin A1) in nutrient-rich medium or under starvation conditions. Tumor protein 53-induced nuclear protein 1 (TP53INP1) was identified as a potential novel target of miR-221 by bioinformative prediction. The protein expression of TP53INP1 was inversely regulated by miR-221 in CRC cells. Furthermore, luciferase activity assays were performed and indicated that miR-221 may regulate the luciferase activity of wild-type TP53INP1 without interfering with the activity of mutant TP53INP1. These data suggested that miR-221 may promote the cell proliferation of CRC via the inhibition of autophagy and targeted TP53INP1.

Our previous work reported the association between two single nucleotide polymorphisms (SNPs) in PIN1 promoter and nasopharyngeal carcinoma (NPC) risk with a small sample size in a low incidence area. This study investigated the association between the two SNPs and NPC risk in 733 patients and 895 controls from a high incidence area. The results indicated the genotype and allele frequencies of -842G > C and -667C > T were both significantly different between patients and controls even using the resampling statistics. The -842GC and -667TT genotypes showed a significantly increased risk of NPC (OR = 1.977, 95% CI = 1.339–2.919, P = 0.001 and OR = 1.438, 95% CI = 1.061–1.922, P = 0.019, respectively). Compared to the most common -842G-667C haplotype, -842G-667T haplotype and -842C-667C haplotype showed a significantly increased risk of NPC (OR = 1.215, 95% CI = 1.053–1.402, P = 0.008 and OR = 2.268, 95% CI = 1.530–3.362, P = 0.001, respectively). Further reporter gene expression suggested that variant -842C-667C and -842G-667T were associated with an enhanced transcriptional activity. In conclusion, our findings suggest that -842G > C and -667C > T in PIN1 promoter are associated with NPC risk; as well as the promoter activity is mediated by functional PIN1 variants.