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30 results on '"Hua, Xiaoliang"'

9. Resolvin D1 Attenuates Inflammation and Pelvic Pain Associated with EAP by Inhibiting Oxidative Stress and NLRP3 Inflammasome Activation via the Nrf2/HO-1 Pathway

Author

Zhang,Jiong, Chen,Juan, Jiang,Qing, Feng,Rui, Zhao,Xiaohu, Li,Haolin, Yang,Cheng, Hua,Xiaoliang, Zhang,Jiong, Chen,Juan, Jiang,Qing, Feng,Rui, Zhao,Xiaohu, Li,Haolin, Yang,Cheng, and Hua,Xiaoliang

Abstract

Jiong Zhang,1,2,* Juan Chen,3,* Qing Jiang,1,* Rui Feng,2 Xiaohu Zhao,2 Haolin Li,2 Cheng Yang,2 Xiaoliang Hua1 1Department of Urology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Department of Urology, the First Affiliated Hospital of Anhui Medical University, Hefei, People’s Republic of China; 3The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People’s Republic of China*These authors contributed equally to this workCorrespondence: Cheng Yang, Department of Urology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Shushan District, Hefei, 230022, People’s Republic of China, Tel +86 0551 6292 3440, Fax +86 551 6363 3742, Email chengyang_md@163.com Xiaoliang Hua, Department of Urology, the Second Affiliated Hospital of Chongqing Medical University, No. 74 Linjiang Road, Yuzhong District, Chongqing, 400010, People’s Republic of China, TelFax +86 023 6369 3222, Email hua_xiaoliang@cqmu.edu.cnBackground: Resolvin D1 (RvD1), a member of the specialized pro-resolving lipid mediators family, has a potent anti-inflammatory effect and alleviates tissue damage. The purpose of the current research was to study the effect of RvD1 on CP/CPPS and the underlying mechanisms using a mouse model of experimental autoimmune prostatitis (EAP) mice.Materials and Methods: The EAP mouse model was successfully established, and was used to test the therapeutic effect of RvD1. Hematoxylin-eosin staining and dihydroethidium staining were used to evaluate the histological changes and oxidative stress levels of prostate tissues. Chronic pelvic pain was assessed by applying von Frey filaments to the lower abdomen. The superoxide dismutase enzyme and malondialdehyde levels were detected using enzyme-linked immunosorbent assay (ELISA). The levels of inflammatio

Published
2023

10. PKMYT1, exacerbating the progression of clear cell renal cell carcinoma, is implied as a biomarker for the diagnosis and prognosis

Author

Chen, Juan, Hua, Xiaoliang, Chen, Heying, Qiu, Xiangmin, Xiao, Haibing, Ge, Shengdong, Liang, Chaozhao, and Zhou, Qin

Subjects
renal cell carcinoma, Aging, Epithelial-Mesenchymal Transition, diagnosis, EMT, Membrane Proteins, Cell Biology, Protein Serine-Threonine Kinases, Protein-Tyrosine Kinases, Prognosis, PKMYT1, Cell Line, Up-Regulation, Cell Transformation, Neoplastic, Humans, Carcinoma, Renal Cell, Biomarkers, Research Paper
Abstract

Clear cell renal cell carcinoma (ccRCC) is one of the most lethal urological malignancies with high tumor heterogeneity, and reliable biomarkers are still needed for its diagnosis and prognosis. WEE family kinases function as key regulators of the G2/M transition, have essential roles in maintaining cellular genomic stability and have the potential to be promising therapeutic targets in various tumors. However, the roles of WEE family kinases in ccRCC remain undetermined. In the present study, we first explored multiple public datasets and found that PKMYT1 was up-regulated in both RCC tumors and cell lines. Expression levels of PKMYT1 were highly associated with pathological stage and grade. Kaplan-Meier curves showed that high PKMYT1 expression was associated with lower overall survival and disease-free survival. Receiver operating characteristic curves revealed that the expression of PKMYT1 could better distinguish ccRCC from normal samples. Functional enrichment analysis demonstrated that cell cycle- related pathways and epithelial to mesenchymal transition (EMT) might be potential mechanisms of PKMYT1 in ccRCC tumorigenesis. Moreover, knockdown of PKMYT1 in vitro attenuated the proliferation, migration and invasion of RCC cell lines, promoted cell apoptosis and prevented the EMT phenotype in vitro. In conclusion, our study demonstrated that PKMYT1 has the potential to act as a diagnostic and prognostic biomarker for RCC patients. Targeting PKMYT1 may be considered as a new potential therapeutic method and direction in RCCs.

Published
2021

17. Additional file 1 of Systematic analyses of the role of prognostic and immunological EIF3A, a reader protein, in clear cell renal cell carcinoma

Author

Zhang, Yi, Hua, Xiaoliang, Shi, Haoqiang, Zhang, Li, Xiao, Haibing, and Liang, Chaozhao

Abstract

Additional file 1: Table S1. Gene sets enrichment analysis of high EIF3A mRNA expression level in the ccRCC based on KEGG database. Table S2. Gene sets enrichment analysis of high EIF3A mRNA expression level in the ccRCC based on GO database. Table S3. The correlation of analysis between EIF3A and relate genes markers of immune cells in TIMER datase. Figure S1. The expression level of EIF3A in colon para-cancerous tissues and pancreatic para-cancerous tissues; Immunohistochemical (IHC) analysis of EIF3A expression in colon para-cancerous tissues (a) and pancreatic para-cancerous tissues(b). Figure S2. Eif3A was expressed in Myeloid-derived suppressor cells and Regulatory T cells by immunofluorescence assay; a Myeloid-derived suppressor cells (MDSC) cells (white arrow) were double immunostained with anti-CD11b antibody (red) and anti-EIF3A antibody (green). The cell nuclei were counterstained with DAPI (blue). The co-localization between the two endogenous proteins CD11b and EIF3A is shown in the merge panel. Scale bar, 20 mm; b Regulatory T cells (white arrow) were double immunostained with anti-Foxp3 antibody (red) and anti-EIF3A antibody (green). The cell nuclei were counterstained with DAPI (blue). The co-localization between the two endogenous proteins Foxp3 and EIF3A is shown in the merge panel. Scale bar, 20 mm.

Published
2021
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23. m6A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients.

Author

Qiu, Xiangmin, Hua, Xiaoliang, Li, Qianyin, Zhou, Qin, and Chen, Juan

Subjects
COVID-19, LEUCOCYTES, IMMUNE checkpoint proteins, METHYLATION, VIRUS diseases
Abstract

Both RNA N6-methyladenosine (m6A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m6A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samples from the GEO database, we disclosed that there is a remarkably higher m6A modification level of blood leukocytes in patients with COVID-19 compared to patients without COVID-19, and this difference was related to CD4+ T cells. Two clusters were identified by unsupervised clustering, m6A cluster A characterized by T cell activation had a higher prognosis than m6A cluster B. Elevated metabolism level, blockage of the immune checkpoint, and lower level of m6A score were observed in m6A cluster B. A protective model was constructed based on nine selected genes and it exhibited an excellent predictive value in COVID-19. Further analysis revealed that the protective score was positively correlated to HFD45 and ventilator-free days, while negatively correlated to SOFA score, APACHE-II score, and crp. Our works systematically depicted a complicated correlation between m6A methylation modification and host lymphocytes in patients infected with SARS-CoV-2 and provided a well-performing model to predict the patients' outcomes. [ABSTRACT FROM AUTHOR]

Published
2021
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30. m 6 A Regulator-Mediated Methylation Modification Patterns and Characteristics of Immunity in Blood Leukocytes of COVID-19 Patients.

Author

Qiu X, Hua X, Li Q, Zhou Q, and Chen J

Subjects
Adenosine metabolism, Cluster Analysis, Computational Biology methods, Disease Susceptibility immunology, Gene Expression Profiling, Humans, Leukocytes metabolism, RNA, Viral metabolism, ROC Curve, Adenosine analogs & derivatives, COVID-19 immunology, COVID-19 virology, Host-Pathogen Interactions immunology, Leukocytes immunology, RNA, Viral genetics, SARS-CoV-2 physiology
Abstract

Both RNA N6-methyladenosine (m 6 A) modification of SARS-CoV-2 and immune characteristics of the human body have been reported to play an important role in COVID-19, but how the m 6 A methylation modification of leukocytes responds to the virus infection remains unknown. Based on the RNA-seq of 126 samples from the GEO database, we disclosed that there is a remarkably higher m 6 A modification level of blood leukocytes in patients with COVID-19 compared to patients without COVID-19, and this difference was related to CD4 + T cells. Two clusters were identified by unsupervised clustering, m 6 A cluster A characterized by T cell activation had a higher prognosis than m 6 A cluster B. Elevated metabolism level, blockage of the immune checkpoint, and lower level of m6A score were observed in m 6 A cluster B. A protective model was constructed based on nine selected genes and it exhibited an excellent predictive value in COVID-19. Further analysis revealed that the protective score was positively correlated to HFD45 and ventilator-free days, while negatively correlated to SOFA score, APACHE-II score, and crp. Our works systematically depicted a complicated correlation between m 6 A methylation modification and host lymphocytes in patients infected with SARS-CoV-2 and provided a well-performing model to predict the patients' outcomes., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Qiu, Hua, Li, Zhou and Chen.)

Published
2021
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