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23 results on '"Hu, Yajuan"'

14. Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway

Author

Huang Chao, Hu Yajuan, Huang Jie, Xu Hao, Tao Dandan, Huang Jingjing, Sun Yan, Hu Lei, and Dong Hongjun

Subjects
Male, MAPK/ERK pathway, MAP Kinase Signaling System, p38 mitogen-activated protein kinases, Apoptosis, Bioengineering, p38 Mitogen-Activated Protein Kinases, Applied Microbiology and Biotechnology, Proinflammatory cytokine, mir-495, Western blot, microRNA, medicine, Humans, Gene silencing, otitis media with effusion, mapk, Cells, Cultured, Cell Proliferation, medicine.diagnostic_test, Cell growth, Chemistry, General Medicine, Up-Regulation, Cell biology, MicroRNAs, inflammation, Case-Control Studies, neat1, Female, RNA, Long Noncoding, TP248.13-248.65, Research Article, Research Paper, Biotechnology
Abstract

Long non-coding RNA (lncRNA) plays a vital role in human inflammatory diseases. Our study aimed to investigate the function of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in otitis media with effusion (OME). The mRNA levels of NEAT1 and miR-495 were measured by RT-qPCR. The protein levels of p38 MAPK were detected by western blot. The levels of inflammatory cytokines were examined by ELISA. CCK-8 and flow cytometry assays were used to evaluate the cell viability and apoptosis, respectively. The interaction between NEAT1 and miR-495 was determined by luciferase reporter and RIP assays. NEAT1 was highly expressed in OME, and silencing of NEAT1 facilitated the cell proliferation and suppressed levels of inflammatory cytokines and cell apoptosis in LPS-induced HMEECs. Moreover, miR-495 was confirmed as a downstream target of NEAT1. Functional assays revealed that NEAT1 promoted the OME by targeting miR-495. It was further demonstrated that NEAT1 could activate the p38 MAPK signaling pathway by regulating miR-495, and the p38 MAPK inhibitor restored the effects of NEAT1 overexpression on the inflammation levels, cell proliferation, and apoptosis. Our study revealed that lncRNA NEAT1 served as a ceRNA to activate p38 MAPK signaling by targeting miR-495 in OME, which may offer a new target for OME treatment.

Published
2021

16. Dynamic Changes of Brain Activity in Different Responsive Groups of Patients with Prolonged Disorders of Consciousness.

Author

Chen, Chen, Han, Jinying, Zheng, Shuang, Zhang, Xintong, Sun, Haibo, Zhou, Ting, Hu, Shunyin, Yan, Xiaoxiang, Wang, Changqing, Wang, Kai, and Hu, Yajuan

Subjects
CONSCIOUSNESS disorders, TRANSCRANIAL direct current stimulation, SYMPTOMS, PREFRONTAL cortex, MEDICAL technology
Abstract

As medical technology continues to improve, many patients diagnosed with brain injury survive after treatments but are still in a coma. Further, multiple clinical studies have demonstrated recovery of consciousness after transcranial direct current stimulation. To identify possible neurophysiological mechanisms underlying disorders of consciousness (DOCs) improvement, we examined the changes in multiple resting-state EEG microstate parameters after high-definition transcranial direct current stimulation (HD-tDCS). Because the left dorsolateral prefrontal cortex is closely related to consciousness, it is often chosen as a stimulation target for tDCS treatment of DOCs. A total of 21 patients diagnosed with prolonged DOCs were included in this study, and EEG microstate analysis of resting state EEG datasets was performed on all patients before and after interventions. Each of them underwent 10 anodal tDCS sessions of the left dorsolateral prefrontal cortex over 5 consecutive working days. According to whether the clinical manifestations improved, DOCs patients were divided into the responsive (RE) group and the non-responsive (N-RE) group. The dynamic changes of resting state EEG microstate parameters were also analyzed. After multiple HD-tDCS interventions, the duration and coverage of class C microstates in the RE group were significantly increased. This study also found that the transition between microstates A and C increased, while the transition between microstates B and D decreased in the responsive group. However, these changes in EEG microstate parameters in the N-RE group have not been reported. Our findings suggest that EEG neural signatures have the potential to assess consciousness states and that improvement in the dynamics of brain activity was associated with the recovery of DOCs. This study extends our understanding of the neural mechanism of DOCs patients in consciousness recovery. [ABSTRACT FROM AUTHOR]

Published
2023
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20. Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway

Author

Hu, Yajuan, primary, Dong, Hongjun, additional, Huang, Jingjing, additional, Huang, Jie, additional, Tao, Dandan, additional, Huang, Chao, additional, Hu, Lei, additional, Xu, Hao, additional, and Sun, Yan, additional

Published
2021
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