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Shui Fu,1 Xian-Guo Wu,2 Liang Zhang,1 Li-Feng Wu,1 Zhang-Mei Luo,1 Qi-Lei Hu1 1Department of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, Hangzhou, Zhejiang, 311100, People’s Republic of China; 2Department of Clinical Laboratory, The Second Affiliated Hospital Zhejiang University School Medicine, Hangzhou, Zhejiang, 310009, People’s Republic of ChinaCorrespondence: Qi-Lei HuDepartment of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, No. 369 Yingbin Road of Nanyuan Street, Linping District, Hangzhou, 311100, People’s Republic of ChinaTel +86 571 89369404Email egglandmine@163.comObjective: To explore the application effect of lean management in improving the quality of outpatient blood collection services.Methods: For this study, a total of 146,907 patients whose blood was sampled by outpatient services between April 2020 and September 2020 were selected. We analyzed the influence of various factors on the waiting time and satisfaction levels of the patients for blood collection and eliminated confounders based on the results of the analysis. Lean management for the outpatient blood collection service was implemented in July 2020. Thus, the 38,275 cases sampled on weekday mornings between April and June 2020 were selected as the ordinary management group, while the 39,473 cases sampled on weekday mornings between July and September 2020 belonged to the lean management group. Finally, the changes in waiting time and the satisfaction levels of the patients were evaluated.Results: The age and gender of the patients and the length of service of the staff, who administered blood collection had a negligible effect on the waiting time (Z=− 1.243, P=0.418; Z=− 1.569, P=0.389; Z = − 1.062, P= 0.563), while there was a statistical difference in the waiting time between different days and different sessions (Z = − 2.581, P = 0.013 and Z = − 4.672, P < 0.001). We also found that the length o

Rong Huang,1 Shui Fu,1 Yin-Jiang Lv,1 Liang Zhang,1 Li-Feng Wu,1 Qi-Lei Hu,1 Juan-Fei Qi2 1Department of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, Hangzhou, 311100, People’s Republic of China; 2Department of Clinical Laboratory, Xinchang Hospital of Traditional Chinese Medicine, Shaoxing, 312500, People’s Republic of ChinaCorrespondence: Juan-Fei QiDepartment of Clinical Laboratory, Xinchang Hospital of Traditional Chinese Medicine, Shaoxing, 312500, People’s Republic of ChinaTel/Fax +86 575-86502591Email qijuanfei_dr@163.comObjective: The present study aims to evaluate the comparability of the results of two methodologies for detecting human chorionic gonadotropin (HCG) to assess whether the immunofluorescence method for detecting HCG is adequate for clinical applications.Methods: Referring to the protocol requirements of the American Clinical Laboratory Standards Institute (CLSI) EP9-A2 (methodological matching and bias assessment with patient samples), we collected 40 fresh serum specimens from our outpatients and inpatients, including 20 specimens with abnormal HCG concentrations (eight samples with different concentration ranges were selected daily and HCG was measured simultaneously with the two testing systems for five consecutive days). The assays were performed on a Dxl 800 fully automated immunoassay analyzer from Beckman Coulter Inc., USA, as a comparative method and on a Jet-iStar 3000 immunoassay analyzer from Zhonghan Shengtai Inc. as an experimental method. Methodological comparison and bias assessment of the results of the two methods for HCG detection were performed. The OLR regression model was used for calculating bias and regression analysis, and Spearman’s rank correlation coefficient was used for correlation analysis. The correlation and comparability of the two systems were calculated based on the results of the analysis.Results: A good correlation in HCG re

Yin-Jiang Lv, Qi-Lei Hu, Rong Huang, Liang Zhang, Li-Feng Wu, Shui Fu Department of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, Hangzhou, Zhejiang, 311100, People’s Republic of ChinaCorrespondence: Shui FuDepartment of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, No. 369 Yingbin Road of Nanyuan Street, Yuhang District, Hangzhou, 311100, People’s Republic of ChinaTel +86 571 89369404Email fushuiolo@163.comObjective: This study explores the significance of serum amyloid A (SAA), C-reactive protein (CRP), and white blood cell (WBC) in the diagnosis and treatment of diarrhea in infants.Methods: Specimens were collected from 126 children with diarrhea and 66 healthy children undergoing health examination. According to the results of stool culture and rotavirus (RV) antigen, these children were divided into three groups: rotavirus group (70 cases), bacterial infection (56 cases), and control groups (66 cases). On the fourth day of admission, children in the RV group underwent stool culture again. Based on the subsequent results, they were further divided into two groups, ie, no secondary bacterial infection and secondary bacterial infection groups. The levels of RV antigen, bacterial antigen, SAA, CRP, and WBC were detected in all children. Then, ROC curve analysis was performed to determine the diagnostic efficacy of SAA, CRP and WBC.Results: The levels of SAA, CRP, and WBC for the RV group were lower than those of the bacterial infection group, but higher compared with the control group (P< 0.05). The diagnostic efficacy of SAA was higher than that of CRP and WBC, with the area under the curve of 0.876, 0.803, and 0.765, respectively. The positive and negative predictive values, specificity, and sensitivity of SAA were slightly better compared with CRP and WBC. The SAA, CRP, and WBC levels of children with a bacterial infection in the RV group on the fourth and seventh days after admission w

Qi-Lei Hu, Shui Fu, Rong Huang, Liang Zhang, Li-Feng Wu, Yin-Jiang Lv Department of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, Hangzhou, Zhejiang, 311100, People’s Republic of ChinaCorrespondence: Yin-Jiang LvDepartment of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, No. 369 Yingbin Road of Nanyuan Street, Yuhang District, Hangzhou, 311100, People’s Republic of ChinaTel +86 571 89369404Email Lvyj6217@163.comObjective: The present study aimed to explore the clinical value of serum amyloid A (SAA) in the diagnosis, treatment, and assessment of ankylosing spondylitis (AS).Methods: Seventy-eight patients with AS were enrolled as the case group, while the control group consisted of 80 healthy individuals enrolled during the same time period. According to the criteria of the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), patients in the case group were divided into those in the remission phase (36 patients) and those in the active phase (42 patients). Levels of SAA, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) were measured in all enrolled subjects and analyzed.Results: SAA levels were significantly higher in the AS group (39.65 ± 12.32 ng/mL) than in the control group (7.64 ± 1.32 ng/mL) (p =0.011) and in patients in the active phase (56.18 ± 17.25 ng/mL) compared with those in the remission phase (20.36 ± 5.36 ng/mL) (p =0.015). The sensitivity and specificity of SAA were 79.49% and 77.50%, respectively. There was a positive correlation between SAA level and the BASDAI grade (r = 0.77, p =0.005), CRP level (r = 0.68, p =0.011), and ESR (r = 0.62, p =0.012).Conclusion: Not only is SAA a reliable indicator for the presence of AS, it may also be useful for monitoring the activity of this disease.Keywords: ankylosing spondylitis, serum amyloid A, C-reactive protein, erythrocyte sedimenta

Shui Fu,1 Miao-Miao Zhang,2 Liang Zhang,1 Li-Feng Wu,1 Qi-Lei Hu1 1Department of Clinical Laboratory, The First People’s Hospital of Yuhang District, Hangzhou, Zhejiang, 311100, People’s Republic of China; 2Department of Emergency, The Fifth People’s Hospital of Yuhang District, Hangzhou, Zhejiang, 311100, People’s Republic of ChinaCorrespondence: Qi-Lei HuThe First People’s Hospital of Yuhang District, No. 369 Yingbin Road of Nanyuan Street, Yuhang District, Hangzhou, 311100, People’s Republic of ChinaTel +86 571 89369404Email huqilaigm@163.comObjective: The aim of this study was to explore the value of serum amyloid A protein (SAA) and neutrophil-to-lymphocyte ratio (NLR) testing in the diagnosis and treatment of children with influenza A.Methods: Specimens were collected from 85 children with influenza A, 85 children with a bacterial infection, and 86 healthy children. The levels of SAA and C-reactive protein (CRP) were measured, and routine blood tests were performed.Results: The levels of SAA and CRP in the bacterial infection group were significantly higher than those in the influenza A group, and the levels in the influenza A group were higher than those in the healthy children. The NLR level in the influenza A group was not different from that in the bacterial infection group, but the NLR levels in the influenza A group and the bacterial infection group were higher than that in the healthy controls. The number of white blood cell (WBC) in the influenza A group was not different from that in healthy children, while the WBC counts in the control and bacterial infection groups were higher than that in the influenza A group. The distribution width of red blood cells in the bacterial infection group was higher than that in healthy controls. The receiver operating characteristic curve analysis showed that the area under the curve for the diagnoses of influenza A for SAA, NLR, and CRP was 0.806, 0.768, and 0.699, respectively. The sensitivity, specifici