Search

Your search keyword '"Hu, Chun-jie"' showing total 11 results
Start Over Author "Hu, Chun-jie"
11 results on '"Hu, Chun-jie"'

2. High-sensitivity surface plasmon resonance sensor based on the ten-fold eccentric core quasi-D-shaped photonic quasi-crystal fiber coated with indium tin oxide

Author

Jiang Yu, HU Chun-jie, Liu Chao, Liu Qiang, Tai Sheng-nan, Zhao Jin, LU Wen-shu, Chu Paul K, LV Jing-wei, Sun Yu-dan, and YI Zao

Subjects
Core (optical fiber), Materials science, Fold (higher-order function), business.industry, Quasicrystal, Optoelectronics, Eccentric, Fiber, Photonics, business, Sensitivity (electronics), Atomic and Molecular Physics, and Optics, Indium tin oxide
Published
2022

5. Cardiac disorder in hemodialysis: benefits of Chinese herbs

Author

Lǚ, Zhong Qing, Sun, Min, Hu, Chun Jie, and Tong, Yan Qing

Subjects
Hemodialysis, Cardiac arrest, Chinese herbs
Abstract

Background: A major cause of mortality in hemodialysis patients is cardiac disease. Most complementary and alternative therapies, including Chinese herbal medicine, have been useful in the treatment of cardiac disorders.Materials and Methods: A 46 year old Asian woman with chronic renal failure was admitted to the clinic for hemodialysis. In the course of the fifth session of standard dialysis, she developed shock followed by a ventricular tachycardia which rapidly degenerated into cardiac arrest, from which she was resuscitated through cardio-pulmonary resuscitation. The following therapeutic strategies were applied: low discharge oxygen inhalation; stricter water and salt restriction; dialysate temperature set at 36.0 OC; rhEPO 3000u, per week, low molecular weight iron dextran, 200mg/day, intravenously for five days; the patient received Chinese herbal concoction orally.Results: The patient obtained efficient standard dialysis without any cardiac syndrome.Conclusion: Chinese herbs are useful in the management of cardiac disorders in hemodialysis. Chinese herbs may provide more benefits by adjusting dialysis strategies.Keywords: Hemodialysis; Cardiac arrest; Chinese herbs

Published
2016

10. Dihydroartemisinin induces apoptosis of cervical cancer cells via upregulation of RKIP and downregulation of bcl-2

Author

Hu, Chun-jie, Zhou, Lei, and Cai, Yan

Abstract

Treatment of recurrent and metastatic cervical cancer remains a challenge, especially in developing countries, which lack efficient screening programs. In recent years, artemisinin and its derivatives, such as dihydroartemisinin (DHA), which were traditionally used as anti-malarial agent, have been shown to inhibit tumor growth with low toxicity to normal cells. In this study, we investigated mechanisms underlying the anti-tumor effect of DHA in cervical cancer. We evaluated the role of DHA on the expression of bcl-2 and Raf kinase inhibitor protein (RKIP), which is a suppressor of metastasis. The MTT assay was used to compare the proliferation of untreated and DHA-treated Hela and Caski cervical cancer cells. Flow cytometry was used to determine the percentage of cells at each stage of the cell cycle in untreated and DHA-treated cells. We used RT-PCR and western blots to determine the expression of bcl-2 and RKIP mRNA and proteins. We evaluated the effect of DHA treatment in nude mice bearing Hela or Caski tumors. DHA-treated cells showed a time- and dose-dependent inhibition of proliferation and a significant increase in apoptosis. The expression of RKIP was significantly upregulated and the expression of bcl-2 was significantly downregulated in DHA-treated cells compared with control cells. DHA treatment caused (1) a significant inhibition of tumor growth and (2) a significant increase in the apoptotic index in nude mice bearing Hela or Caski tumors. Our data suggest that DHA inhibits cervical cancer growth via upregulation of RKIP and downregulation of bcl-2.

Published
2014
Full Text
View/download PDF

11. [Growth inhibitory effects of lipofectamine-mediated DCC gene on ovarian epithelial carcinoma].

Author

Li PL, Hu CJ, Li CM, Meng CY, and Gao L

Subjects
Cell Cycle genetics, Cell Cycle physiology, Cell Line, Tumor, DCC Receptor, Female, Genetic Vectors chemistry, Genetic Vectors genetics, Humans, Immunohistochemistry, Ovarian Neoplasms genetics, Ovarian Neoplasms pathology, Ovarian Neoplasms physiopathology, RNA, Messenger biosynthesis, RNA, Messenger genetics, Receptors, Cell Surface biosynthesis, Receptors, Cell Surface physiology, Reverse Transcriptase Polymerase Chain Reaction, Transfection methods, Tumor Suppressor Proteins biosynthesis, Tumor Suppressor Proteins physiology, Cell Proliferation, Lipids chemistry, Receptors, Cell Surface genetics, Tumor Suppressor Proteins genetics
Abstract

Objective: To study the inhibitory effects of lipofectamine-mediated deleted colorectal carcinoma gene on ovarian epithelial carcinoma (ovarian cancer) cell line SKOV3., Methods: We constructed a recombinant eukaryotic expression vector pcDNA3.1 (+)-DCC containing exogenous human DCC cDNA and vector with neomycin resistance gene, which were introduced by lipofectamine-mediated gene transfection into SKOV3 cell line that does not express DCC endogenously, thus forming SKOV3/DCC. Therefore, the experimental cells were classified into SKOV3/DCC, SKOV3/Neo and SKOV3. By using reverse transcriptase-polymerase chain reaction and immunocytochemistry, the expression of DCC mRNA and its protein were examined., Results: Exogenous DCC had successfully been transferred into SKOV3 cells and obtained permanent expression. The growth speed of SKOV3/DCC was slower than the other two groups, there was significant difference between them (P < 0.01). SKOV3/DCC clones number was 38 +/- 8, while SKOV3 and SKOV3/Neo were 192 +/- 8 and 186 +/- 10, respectively, there was significant difference between them (P < 0.01). The percentage of G(1) phase cells increased to 78.0%, which that of S phase decreased to 5.3% by analyzing cell cycle, there was significant difference between them (20.0% and 3.2%, P < 0.01). The ultrastructural changes of the cells were observed under electron microscope, revealing growth retardation., Conclusion: DCC gene played an important role in generation and development of ovarian carcinomas.

Published
2006

Catalog

Books, media, physical & digital resources
See catalog results