Search

Your search keyword '"Hu, Chiung-Wen"' showing total 249 results
Start Over Author "Hu, Chiung-Wen"
249 results on '"Hu, Chiung-Wen"'

16. A Novel Adductomics Workflow Incorporating FeatureHunterSoftware: Rapid Detection of Nucleic Acid Modifications for Studying the Exposome

Author

Hu, Chiung-Wen, Chang, Yuan-Jhe, Chang, Wei-Hung, Cooke, Marcus S., Chen, Yet-Ran, and Chao, Mu-Rong

Abstract

Exposure to the physicochemical agents that interact with nucleic acids (NA) may lead to modification of DNA and RNA (i.e., NA modifications), which have been associated with various diseases, including cancer. The emerging field of NA adductomics aims to identify both known and unknown NA modifications, some of which may also be associated with proteins. One of the main challenges for adductomics is the processing of massive and complex data generated by high-resolution tandem mass spectrometry (HR-MS/MS). To address this, we have developed a software called “FeatureHunter”, which provides the automated extraction, annotation, and classification of different types of key NA modifications based on the MS and MS/MS spectra acquired by HR-MS/MS, using a user-defined feature list. The capability and effectiveness of FeatureHunterwas demonstrated by analyzing various NA modifications induced by formaldehyde or chlorambucil in mixtures of calf thymus DNA, yeast RNA and proteins, and by analyzing the NA modifications present in the pooled urines of smokers and nonsmokers. The incorporation of FeatureHunterinto the NA adductomics workflow offers a powerful tool for the identification and classification of various types of NA modifications induced by reactive chemicals in complex biological samples, providing a valuable resource for studying the exposome.

Published
2024
Full Text
View/download PDF

37. Biomarkers of nucleic acid oxidation:A summary state-of-the-art

Author

Chao, Mu-Rong, Evans, Mark D., Hu, Chiung-Wen, Ji, Yunhee, Møller, Peter, Rossner, Pavel, Cooke, Marcus S., Chao, Mu-Rong, Evans, Mark D., Hu, Chiung-Wen, Ji, Yunhee, Møller, Peter, Rossner, Pavel, and Cooke, Marcus S.

Abstract

Oxidatively generated damage to DNA has been implicated in the pathogenesis of a wide variety of diseases. Increasingly, interest is also focusing upon the effects of damage to the other nucleic acids, RNA and the (2?deoxy-)ribonucleotide pools, and evidence is growing that these too may have an important role in disease. LCMS/MS has the ability to provide absolute quantification of specific biomarkers, such as 8-oxo-7,8-dihydro-2?deoxyGuo (8-oxodG), in both nuclear and mitochondrial DNA, and 8-oxoGuo in RNA. However, significant quantities of tissue are needed, limiting its use in human biomonitoring studies. In contrast, the comet assay requires much less material, and as little as 5 ?L of blood may be used, offering a minimally invasive means of assessing oxidative stress in vivo, but this is restricted to nuclear DNA damage only. Urine is an ideal matrix in which to non-invasively study nucleic acid-derived biomarkers of oxidative stress, and considerable progress has been made towards robustly validating these measurements, not least through the efforts of the European Standards Committee on Urinary (DNA) Lesion Analysis. For urine, LC-MS/MS is considered the gold standard approach, and although there have been improvements to the ELISA methodology, this is largely limited to 8oxodG. Emerging DNA adductomics approaches, which either comprehensively assess the totality of adducts in DNA, or map DNA damage across the nuclear and mitochondrial genomes, offer the potential to considerably advance our understanding of the mechanistic role of oxidatively damaged nucleic acids in disease.

Published
2021

Catalog

Books, media, physical & digital resources
See catalog results