2,382 results on '"Hsueh, Wei"'
Search Results
2. Indigofera suffruticosa aerial parts extract induce G2/M arrest and ATR/CHK1 pathway in Jurkat cells
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Tran, Hong-Loan, Lai, Kuei-Hung, Chang, Hsun-Shuo, Chen, Yi-Siao, Wang, Hui-Chun, Yang, Shuen-Shin, Chang, Hsueh-Wei, Hsu, Chin-Mu, Yen, Chia-Hung, and Hsiao, Hui-Hua
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- 2024
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3. VoPiFNet: Voxel-Pixel Fusion Network for Multi-Class 3D Object Detection.
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Chia-Hung Wang, Hsueh-Wei Chen, Yi Chen, Pei-Yung Hsiao, and Li-Chen Fu
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- 2024
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4. Indigofera suffruticosa aerial parts extract induce G2/M arrest and ATR/CHK1 pathway in Jurkat cells
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Hong-Loan Tran, Kuei-Hung Lai, Hsun-Shuo Chang, Yi-Siao Chen, Hui-Chun Wang, Shuen-Shin Yang, Hsueh-Wei Chang, Chin-Mu Hsu, Chia-Hung Yen, and Hui-Hua Hsiao
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Folk medicine ,Indigofera suffruticosa ,Acute lymphoblastic leukemia ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background Indigofera suffruticosa Mill. is used as a folk medicine for treating patients with leukemia, however very little is known regarding the molecular mechanism of its anti-leukemic activity and the chemical profile of the active extract. The present study aimed to reveal the molecular effect of I. suffruticosa aerial parts extract (ISAE) on leukemia cells and its chemical constituents. Methods Cytotoxicity of ISAE were determined by resazurin viability assay, multitox – Glo multiplex cytotoxicity assay, and Annexin V staining assay. Cell cycle profiles were revealed by propidium iodide staining assay. The effects of ISAE on G2/M arrest signaling and DNA damage were evaluated by Western blot assay and phospho-H2A.X staining assay. The chemical profile of ISAE were determined by tandem mass spectroscopy and molecular networking approach. Results We showed that the acute lymphoblastic leukemia cell line Jurkat cell was more responsive to ISAE treatment than other leukemia cell lines. In contrast, ISAE did not induce cytotoxic effects in normal fibroblast cells. Cell cycle analysis revealed that ISAE triggered G2/M arrest in Jurkat cells in dose- and time-dependent manners. Elevation of annexin V-stained cells and caspase 3/7 activity suggested ISAE-induced apoptosis. Furthermore, ISAE alone could increase the phosphorylation of CDK1 at Y15 and activate the ATR/CHK1/Wee1/CDC25C signaling pathway. However, the addition of caffeine, a widely used ATR inhibitor to ISAE, reduced the phosphorylation of ATR, CHK1, and CDK1, as well as G2/M arrest in Jurkat cells. Moreover, increased phospho-H2A.X stained cells indicated the involvement of DNA damage in the anti-leukemic effect of ISAE. Finally, qualitative analysis using UPLC-tandem mass spectroscopy and molecular networking revealed that tryptanthrin was the most abundant organoheterocyclic metabolite in ISAE. At equivalent concentrations to ISAE, tryptanthrin induced G2/M arrest of Jurkat cells, which can be prevented by caffeine. Conclusions ISAE causes G2/M arrest via activating ATR/CHK1/CDK1 pathway and tryptanthrin is one of the active components of ISAE. Our findings provide subtle support to the traditional use of I. suffruitcosa in leukemia management in folk medicine.
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- 2024
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5. Marine anticancer drugs in modulating miRNAs and antioxidant signaling
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Chuang, Ya-Ting, Yen, Ching-Yu, Tang, Jen-Yang, Wu, Kuo-Chuan, Chang, Fang-Rong, Tsai, Yi-Hong, Chien, Tsu-Ming, and Chang, Hsueh-Wei
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- 2024
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6. Raman Vibrational Signatures of Excited States of Echinenone in the Orange Carotenoid Protein (OCP) and Implications for its Photoactivation Mechanism
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Chrupková, Petra, van Stokkum, Ivo H.M., Friedrich, Thomas, Moldenhauer, Marcus, Budisa, Nediljko, Tseng, Hsueh-Wei, Polívka, Tomáš, Cherepanov, Dmitry A., Maksimov, Eugene G., and Kloz, Miroslav
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- 2024
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7. VPFNet: Voxel-Pixel Fusion Network for Multi-class 3D Object Detection
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Wang, Chia-Hung, Chen, Hsueh-Wei, and Fu, Li-Chen
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Computer Science - Computer Vision and Pattern Recognition ,Computer Science - Artificial Intelligence ,Computer Science - Machine Learning ,Computer Science - Robotics - Abstract
Many LiDAR-based methods for detecting large objects, single-class object detection, or under easy situations were claimed to perform quite well. However, their performances of detecting small objects or under hard situations did not surpass those of the fusion-based ones due to failure to leverage the image semantics. In order to elevate the detection performance in a complicated environment, this paper proposes a deep learning (DL)-embedded fusion-based multi-class 3D object detection network which admits both LiDAR and camera sensor data streams, named Voxel-Pixel Fusion Network (VPFNet). Inside this network, a key novel component is called Voxel-Pixel Fusion (VPF) layer, which takes advantage of the geometric relation of a voxel-pixel pair and fuses the voxel features and the pixel features with proper mechanisms. Moreover, several parameters are particularly designed to guide and enhance the fusion effect after considering the characteristics of a voxel-pixel pair. Finally, the proposed method is evaluated on the KITTI benchmark for multi-class 3D object detection task under multilevel difficulty, and is shown to outperform all state-of-the-art methods in mean average precision (mAP). It is also noteworthy that our approach here ranks the first on the KITTI leaderboard for the challenging pedestrian class.
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- 2021
8. Ferroptosis-Regulated Natural Products and miRNAs and Their Potential Targeting to Ferroptosis and Exosome Biogenesis
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Ya-Ting Chuang, Ching-Yu Yen, Tsu-Ming Chien, Fang-Rong Chang, Yi-Hong Tsai, Kuo-Chuan Wu, Jen-Yang Tang, and Hsueh-Wei Chang
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exosome ,miRNA ,ferroptosis ,exosome biogenesis ,natural products ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Ferroptosis, which comprises iron-dependent cell death, is crucial in cancer and non-cancer treatments. Exosomes, the extracellular vesicles, may deliver biomolecules to regulate disease progression. The interplay between ferroptosis and exosomes may modulate cancer development but is rarely investigated in natural product treatments and their modulating miRNAs. This review focuses on the ferroptosis-modulating effects of natural products and miRNAs concerning their participation in ferroptosis and exosome biogenesis (secretion and assembly)-related targets in cancer and non-cancer cells. Natural products and miRNAs with ferroptosis-modulating effects were retrieved and organized. Next, a literature search established the connection of a panel of ferroptosis-modulating genes to these ferroptosis-associated natural products. Moreover, ferroptosis-associated miRNAs were inputted into the miRNA database (miRDB) to bioinformatically search the potential targets for the modulation of ferroptosis and exosome biogenesis. Finally, the literature search provided a connection between ferroptosis-modulating miRNAs and natural products. Consequently, the connections from ferroptosis–miRNA–exosome biogenesis to natural product-based anticancer treatments are well-organized. This review sheds light on the research directions for integrating miRNAs and exosome biogenesis into the ferroptosis-modulating therapeutic effects of natural products on cancer and non-cancer diseases.
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- 2024
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9. Marine Prostanoids with Cytotoxic Activity from Octocoral Clavularia spp.
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Ming-Ya Cheng, I-Chi Hsu, Shi-Ying Huang, Ya-Ting Chuang, Tzi-Yi Ke, Hsueh-Wei Chang, Tian-Huei Chu, Ching-Yeu Chen, and Yuan-Bin Cheng
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prostanoid ,Clavularia spp. ,cytotoxicity ,Ca9-22 ,Biology (General) ,QH301-705.5 - Abstract
Octocoral of the genus Clavularia is a kind of marine invertebrate possessing abundant cytotoxic secondary metabolites, such as prostanoids and dolabellanes. In our continuous natural product study of C. spp., two previously undescribed prostanoids [clavulone I-15-one (1) and 12-O-deacetylclavulone I (2)] and eleven known analogs (3–13) were identified. The structures of these new compounds were elucidated based on analysis of their 1D and 2D NMR, HRESIMS, and IR data. Additionally, all tested prostanoids (1 and 3–13) showed potent cytotoxic activities against the human oral cancer cell line (Ca9-22). The major compound 3 showed cytotoxic activity against the Ca9-22 cells with the IC50 value of 2.11 ± 0.03 μg/mL, which echoes the cytotoxic effect of the coral extract. In addition, in silico tools were used to predict the possible effects of isolated compounds on human tumor cell lines and nitric oxide production, as well as the pharmacological potentials.
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- 2024
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10. Distinct Failure Patterns in Hypopharyngeal Cancer Patients Receiving Surgery-Based Versus Radiation-Based Treatment
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Lin, Yu-Hsuan, Hsiao, Jenn-Ren, Wu, Yuan-Hua, Chang, Jeffrey S., Ou, Chun-Yen, Lee, Wei-Ting, Huang, Cheng-Chih, Chang, Chan-Chi, Lai, Yu-Hsuan, Tsai, Sen-Tien, Hsueh, Wei-Ting, Yen, Chia-Jui, Lin, Chen-Lin, Chen, Yu-Shan, Jiang, Shih-Sheng, Su, Yu-Chu, and Wu, Shang-Yin
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- 2023
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11. Nakamusines A−C, new 9-methyladeninium diterpenoid alkaloids from a Formosan marine sponge Agelas nakamurai
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Fu, Chung-Wei, Chiang, Lin, Chao, Chih-Hua, Huang, Yen-Lin, Chiou, Shu-Fen, Wang, Liang-Chun, Chang, Hsueh-Wei, Chen, Shu-Li, Wang, Hui-Chun, Yu, Meng-Chen, Huang, Hui-Chi, and Sheu, Jyh-Horng
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- 2023
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12. Michelia compressa-Derived Santamarine Inhibits Oral Cancer Cell Proliferation via Oxidative Stress-Mediated Apoptosis and DNA Damage
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Hsin-I Lu, Kuan-Liang Chen, Ching-Yu Yen, Chung-Yi Chen, Tsu-Ming Chien, Chih-Wen Shu, Yu-Hsuan Chen, Jiiang-Huei Jeng, Bing-Hung Chen, and Hsueh-Wei Chang
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evergreen tree ,natural product ,oxidative stress ,apoptosis ,oral cancer ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
The anti-oral cancer effects of santamarine (SAMA), a Michelia compressa var. compressa-derived natural product, remain unclear. This study investigates the anticancer effects and acting mechanism of SAMA against oral cancer (OC-2 and HSC-3) in parallel with normal (Smulow–Glickman; S-G) cells. SAMA selectively inhibits oral cancer cell viability more than normal cells, reverted by the oxidative stress remover N-acetylcysteine (NAC). The evidence of oxidative stress generation, such as the induction of reactive oxygen species (ROS) and mitochondrial superoxide and the depletion of mitochondrial membrane potential and glutathione, further supports this ROS-dependent selective antiproliferation. SAMA arrests oral cancer cells at the G2/M phase. SAMA triggers apoptosis (annexin V) in oral cancer cells and activates caspases 3, 8, and 9. SAMA enhances two types of DNA damage in oral cancer cells, such as γH2AX and 8-hydroxy-2-deoxyguanosine. Moreover, all of these anticancer mechanisms of SAMA are more highly expressed in oral cancer cells than in normal cells in concentration and time course experiments. These above changes are attenuated by NAC, suggesting that SAMA exerts mechanisms of selective antiproliferation that depend on oxidative stress while maintaining minimal cytotoxicity to normal cells.
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- 2024
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13. 6-n-Butoxy-10-nitro-12,13-dioxa-11-azatricyclo[7.3.1.02,7]trideca-2,4,6,10-tetraene Improves the X-ray Sensitivity on Inhibiting Proliferation and Promoting Oxidative Stress and Apoptosis of Oral Cancer Cells
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Kun-Han Yang, Ching-Yu Yen, Sheng-Chieh Wang, Fang-Rong Chang, Meng-Yang Chang, Chieh-Kai Chan, Jiiang-Huei Jeng, Jen-Yang Tang, and Hsueh-Wei Chang
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dioxabicyclo[3.3.1]nonane ,oral cancer ,radiosensitization ,oxidative stress ,Biology (General) ,QH301-705.5 - Abstract
This in vitro study examines the anti-oral cancer effects and mechanisms of a combined X-ray/SK2 treatment, i.e., X-ray and 6-n-butoxy-10-nitro-12,13-dioxa-11-azatricyclo[7.3.1.02,7]trideca-2,4,6,10-tetraene (SK2). ATP cell viability and flow cytometry-based cell cycle, apoptosis, oxidative stress, and DNA damage assessments were conducted. The X-ray/SK2 treatment exhibited lower viability in oral cancer (Ca9-22 and CAL 27) cells than in normal (Smulow–Glickman, S-G) cells, i.e., 32.0%, 46.1% vs. 59.0%, which showed more antiproliferative changes than with X-ray or SK2 treatment. Oral cancer cells under X-ray/SK2 treatment showed slight subG1 and G2/M increments and induced high annexin V-monitored apoptosis compared to X-ray or SK2 treatment. The X-ray/SK2 treatment showed higher caspase 3 and 8 levels for oral cancer cells than other treatments. X-ray/SK2 showed a higher caspase 9 level in CAL 27 cells than other treatments, while Ca9-22 cells showed similar levels under X-ray and/or SK2. The X-ray/SK2 treatment showed higher reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) depletion than other treatments. Meanwhile, the mitochondrial superoxide (MitoSOX) and glutathione levels in X-ray/SK2 treatment did not exhibit the highest rank compared to others. Moreover, oral cancer cells had higher γH2AX and/or 8-hydroxy-2-deoxyguanosine levels from X-ray/SK2 treatment than others. All these measurements for X-ray/SK2 in oral cancer cells were higher than in normal cells and attenuated by N-acetylcysteine. In conclusion, X-ray/SK2 treatment showed ROS-dependent enhanced antiproliferative, apoptotic, and DNA damage effects in oral cancer cells with a lower cytotoxic influence on normal cells.
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- 2024
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14. Prognostic value of renal function for upper tract urothelial carcinoma who underwent radical nephroureterectomy: Sex differences
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Tsu-Ming Chien, Ching-Chia Li, Yen-Man Lu, Hsueh-Wei Chang, Yii-Her Chou, and Wen-Jeng Wu
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Renal function ,Urothelial carcinoma ,Radical nephrourete-rectomy ,Medicine (General) ,R5-920 - Abstract
Background/purpose: Upper tract urothelial carcinoma (UTUC) is a relatively rare type of urothelial carcinoma. Additionally, only few reports have examined the sex differences in patients with UTUC. Therefore, the present study aimed to identify the sex factors affecting renal function in patients with UTUC who underwent radical nephroureterectomy (RNU). Methods: Patients who underwent RNU for non-metastatic UTUC between 2000 and 2013 were retrospectively reviewed and divided into two groups by sex. The Kaplan–Meier method was applied to evaluate the effects of sex on survival, whereas for the other clinicopathological parameters, hazard ratios were evaluated using the Cox regression model. The analyses were also performed in patients with different chronic kidney disease (CKD) stages. Results: A total of 368 patients were included, 147 men and 221 women. Female patients had a higher rate of anemia, advanced CKD stage, and dialysis. Male patients predominantly had a higher rate of smoking. The Kaplan–Meier analysis revealed no differences between sexes on recurrence-free survival (RFS) and cancer-specific survival (CSS). Multivariate analysis confirmed that ureteral tumors, advanced pathological tumor stage, and adjuvant chemotherapy indicated significantly worse survival outcomes in both sexes. However, only female patients with advanced CKD showed poorer RFS. After adjusting for renal function, the analysis found men had worse RFS. Conclusion: The female sex is significantly associated with a higher prevalence of advanced CKD stage, and dialysis among patients with UTUC who underwent RNU in our institute. Sex differences in renal function needs to be considered when evaluating survival.
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- 2022
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15. XaMINA: A Real-World, Prospective, Observational Study of Treatment-Naïve Patients Treated with Rivaroxaban for Stroke Prevention in Atrial Fibrillation in Asia
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Liu, Ping-Yen, Choi, Eue-Keun, Kim, Tae-Seok, Kuo, Jen-Yuan, Lee, Jung Myung, On, Young Keun, Park, Sang-Weon, Park, Hyung-Wook, Shin, Dong-Gu, Wang, Lili, Yen, Hsueh-Wei, and Lee, Moon-Hyoung
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- 2022
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16. Marine Prostanoids with Cytotoxic Activity from Octocoral Clavularia spp.
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Cheng, Ming-Ya, primary, Hsu, I-Chi, additional, Huang, Shi-Ying, additional, Chuang, Ya-Ting, additional, Ke, Tzi-Yi, additional, Chang, Hsueh-Wei, additional, Chu, Tian-Huei, additional, Chen, Ching-Yeu, additional, and Cheng, Yuan-Bin, additional
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- 2024
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17. Chemical Constituents from Soft Coral Clavularia spp. Demonstrate Antiproliferative Effects on Oral Cancer Cells
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Ming-Ya Cheng, Ya-Ting Chuang, Hsueh-Wei Chang, Zheng-Yu Lin, Ching-Yeu Chen, and Yuan-Bin Cheng
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eudensamane-type sesquiterpene lactone ,dolabellane ,Clavularia spp. ,cytotoxicity ,Biology (General) ,QH301-705.5 - Abstract
Five new eudensamane-type sesquiterpene lactones, clasamanes A–E (1–5), three new dolabellane-type diterpenes, clabellanes A–C (6–8), and fifteen known compounds (9–23) were isolated from the ethanolic extract of Taiwanese soft coral Clavularia spp. The structures of all undescribed components (1–8) were determined by analysis of IR, mass, NMR, and UV spectroscopic data. The absolute configuration of new compounds was determined by using circular dichroism and DP4+ calculations. The cytotoxic activities of all isolated marine natural products were evaluated. Compound 7 showed a significant cytotoxic effect against oral cancer cell line (Ca9-22) with an IC50 value of 7.26 ± 0.17 μg/mL.
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- 2023
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18. Prognostic value of renal function for upper tract urothelial carcinoma who underwent radical nephroureterectomy: Sex differences
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Chien, Tsu-Ming, Li, Ching-Chia, Lu, Yen-Man, Chang, Hsueh-Wei, Chou, Yii-Her, and Wu, Wen-Jeng
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- 2022
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19. Skin sympathetic nerve activity and ventricular arrhythmias in acute coronary syndrome
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Huang, Tien-Chi, Lin, Shin-Jing, Chen, Chang-Jen, Jhuo, Shih-Jie, Chang, Chien-Wei, Lin, Shih-Ching, Chi, Nai-Yu, Chou, Li-Fang, Tai, Li-Hsin, Liu, Yi-Hsueh, Lin, Tsung-Han, Liao, Wei-Sheng, Kao, Pei-Heng, Cheng, Mu-Chun, Hsu, Po-Chao, Lee, Chee-Siong, Lin, Yi-Hsiung, Lee, Hsiang-Chun, Lu, Ye-Hsu, Yen, Hsueh-Wei, Lin, Tsung-Hsien, Su, Ho-Ming, Lai, Wen-Ter, Dai, Chia-Yen, Lee, Chien-Hung, Chen, Peng-Sheng, Lin, Shien-Fong, and Tsai, Wei-Chung
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- 2022
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20. DeepBarcoding: Deep Learning for Species Classification Using DNA Barcoding.
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Cheng-Hong Yang 0001, Kuo-Chuan Wu, Li-Yeh Chuang, and Hsueh-Wei Chang
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- 2022
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21. Parameterization of a single H-bond in Orange Carotenoid Protein by atomic mutation reveals principles of evolutionary design of complex chemical photosystems
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Marcus Moldenhauer, Hsueh-Wei Tseng, Anastasia Kraskov, Neslihan N. Tavraz, Igor A. Yaroshevich, Peter Hildebrandt, Nikolai N. Sluchanko, Georg A. Hochberg, Lars-Oliver Essen, Nediljko Budisa, Lukas Korf, Eugene G. Maksimov, and Thomas Friedrich
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atomic mutations ,hydrogen bond strength/energy ,non-canonical amino acids ,orthogonal translation ,Orange Carotenoid Protein ,Biology (General) ,QH301-705.5 - Abstract
Introduction: Dissecting the intricate networks of covalent and non-covalent interactions that stabilize complex protein structures is notoriously difficult and requires subtle atomic-level exchanges to precisely affect local chemical functionality. The function of the Orange Carotenoid Protein (OCP), a light-driven photoswitch involved in cyanobacterial photoprotection, depends strongly on two H-bonds between the 4-ketolated xanthophyll cofactor and two highly conserved residues in the C-terminal domain (Trp288 and Tyr201).Method: By orthogonal translation, we replaced Trp288 in Synechocystis OCP with 3-benzothienyl-L-alanine (BTA), thereby exchanging the imino nitrogen for a sulphur atom.Results: Although the high-resolution (1.8 Å) crystal structure of the fully photoactive OCP-W288_BTA protein showed perfect isomorphism to the native structure, the spectroscopic and kinetic properties changed distinctly. We accurately parameterized the effects of the absence of a single H-bond on the spectroscopic and thermodynamic properties of OCP photoconversion and reveal general principles underlying the design of photoreceptors by natural evolution.Discussion: Such “molecular surgery” is superior over trial-and-error methods in hypothesis-driven research of complex chemical systems.
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- 2023
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22. ASO Visual Abstract: Distinct Failure Patterns in Hypopharyngeal Cancer Patients Receiving Surgery-Based Versus Radiation-Based Treatment
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Lin, Yu-Hsuan, Hsiao, Jenn-Ren, Wu, Yuan-Hua, Chang, Jeffrey S., Ou, Chun-Yen, Lee, Wei-Ting, Huang, Cheng-Chih, Chang, Chan-Chi, Lai, Yu-Hsuan, Tsai, Sen-Tien, Hsueh, Wei-Ting, Yen, Chia-Jui, Lin, Chen-Lin, Chen, Yu-Shan, Jiang, Shih-Sheng, Su, Yu-Chu, and Wu, Shang-Yin
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- 2023
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23. Connection between Radiation-Regulating Functions of Natural Products and miRNAs Targeting Radiomodulation and Exosome Biogenesis
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Jen-Yang Tang, Ya-Ting Chuang, Jun-Ping Shiau, Ching-Yu Yen, Fang-Rong Chang, Yi-Hong Tsai, Ammad Ahmad Farooqi, and Hsueh-Wei Chang
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natural products ,miRNA ,exosome ,radiomodulation ,targets ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Exosomes are cell-derived membranous structures primarily involved in the delivery of the payload to the recipient cells, and they play central roles in carcinogenesis and metastasis. Radiotherapy is a common cancer treatment that occasionally generates exosomal miRNA-associated modulation to regulate the therapeutic anticancer function and side effects. Combining radiotherapy and natural products may modulate the radioprotective and radiosensitizing responses of non-cancer and cancer cells, but there is a knowledge gap regarding the connection of this combined treatment with exosomal miRNAs and their downstream targets for radiation and exosome biogenesis. This review focuses on radioprotective natural products in terms of their impacts on exosomal miRNAs to target radiation-modulating and exosome biogenesis (secretion and assembly) genes. Several natural products have individually demonstrated radioprotective and miRNA-modulating effects. However, the impact of natural-product-modulated miRNAs on radiation response and exosome biogenesis remains unclear. In this review, by searching through PubMed/Google Scholar, available reports on potential functions that show radioprotection for non-cancer tissues and radiosensitization for cancer among these natural-product-modulated miRNAs were assessed. Next, by accessing the miRNA database (miRDB), the predicted targets of the radiation- and exosome biogenesis-modulating genes from the Gene Ontology database (MGI) were retrieved bioinformatically based on these miRNAs. Moreover, the target-centric analysis showed that several natural products share the same miRNAs and targets to regulate radiation response and exosome biogenesis. As a result, the miRNA–radiomodulation (radioprotection and radiosensitization)–exosome biogenesis axis in regard to natural-product-mediated radiotherapeutic effects is well organized. This review focuses on natural products and their regulating effects on miRNAs to assess the potential impacts of radiomodulation and exosome biogenesis for both the radiosensitization of cancer cells and the radioprotection of non-cancer cells.
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- 2023
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24. Protein phosphatase 2A modulation and connection with miRNAs and natural products
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Chuang, Ya‐Ting, primary, Yen, Ching‐Yu, additional, Tang, Jen‐Yang, additional, Chang, Fang‐Rong, additional, Tsai, Yi‐Hong, additional, Wu, Kuo‐Chuan, additional, Chien, Tsu‐Ming, additional, and Chang, Hsueh‐Wei, additional
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- 2024
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25. 6-n-Butoxy-10-nitro-12,13-dioxa-11-azatricyclo[7.3.1.02,7]trideca-2,4,6,10-tetraene Improves the X-ray Sensitivity on Inhibiting Proliferation and Promoting Oxidative Stress and Apoptosis of Oral Cancer Cells
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Yang, Kun-Han, primary, Yen, Ching-Yu, additional, Wang, Sheng-Chieh, additional, Chang, Fang-Rong, additional, Chang, Meng-Yang, additional, Chan, Chieh-Kai, additional, Jeng, Jiiang-Huei, additional, Tang, Jen-Yang, additional, and Chang, Hsueh-Wei, additional
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- 2024
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26. Michelia compressa-Derived Santamarine Inhibits Oral Cancer Cell Proliferation via Oxidative Stress-Mediated Apoptosis and DNA Damage
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Lu, Hsin-I, primary, Chen, Kuan-Liang, additional, Yen, Ching-Yu, additional, Chen, Chung-Yi, additional, Chien, Tsu-Ming, additional, Shu, Chih-Wen, additional, Chen, Yu-Hsuan, additional, Jeng, Jiiang-Huei, additional, Chen, Bing-Hung, additional, and Chang, Hsueh-Wei, additional
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- 2024
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27. Physapruin A Exerts Endoplasmic Reticulum Stress to Trigger Breast Cancer Cell Apoptosis via Oxidative Stress
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Tzu-Jung Yu, Jun-Ping Shiau, Jen-Yang Tang, Ammad Ahmad Farooqi, Yuan-Bin Cheng, Ming-Feng Hou, Chia-Hung Yen, and Hsueh-Wei Chang
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withanolide ,ER expansion ,aggresome ,caspase activation ,N-acetylcysteine ,ROS ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Physalis plants are commonly used traditional medicinal herbs, and most of their extracts containing withanolides show anticancer effects. Physapruin A (PHA), a withanolide isolated from P. peruviana, shows antiproliferative effects on breast cancer cells involving oxidative stress, apoptosis, and autophagy. However, the other oxidative stress-associated response, such as endoplasmic reticulum (ER) stress, and its participation in regulating apoptosis in PHA-treated breast cancer cells remain unclear. This study aims to explore the function of oxidative stress and ER stress in modulating the proliferation and apoptosis of breast cancer cells treated with PHA. PHA induced a more significant ER expansion and aggresome formation of breast cancer cells (MCF7 and MDA-MB-231). The mRNA and protein levels of ER stress-responsive genes (IRE1α and BIP) were upregulated by PHA in breast cancer cells. The co-treatment of PHA with the ER stress-inducer (thapsigargin, TG), i.e., TG/PHA, demonstrated synergistic antiproliferation, reactive oxygen species generation, subG1 accumulation, and apoptosis (annexin V and caspases 3/8 activation) as examined by ATP assay, flow cytometry, and western blotting. These ER stress responses, their associated antiproliferation, and apoptosis changes were partly alleviated by the N-acetylcysteine, an oxidative stress inhibitor. Taken together, PHA exhibits ER stress-inducing function to promote antiproliferation and apoptosis of breast cancer cells involving oxidative stress.
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- 2023
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28. Boesenbergia stenophylla-Derived Stenophyllol B Exerts Antiproliferative and Oxidative Stress Responses in Triple-Negative Breast Cancer Cells with Few Side Effects in Normal Cells
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Min-Yu Lee, Jun-Ping Shiau, Jen-Yang Tang, Ming-Feng Hou, Phoebe Sussana Primus, Chai-Lin Kao, Yeun-Mun Choo, and Hsueh-Wei Chang
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Boesenbergia plants ,triple-negative breast cancer ,oxidative stress ,apoptosis ,DNA damage ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Triple-negative breast cancer (TNBC) is insensitive to target therapy for non-TNBC and needs novel drug discovery. Extracts of the traditional herb Boesenbergia plant in Southern Asia exhibit anticancer effects and contain novel bioactive compounds but merely show cytotoxicity. We recently isolated a new compound from B. stenophylla, stenophyllol B (StenB), but the impact and mechanism of its proliferation-modulating function on TNBC cells remain uninvestigated. This study aimed to assess the antiproliferative responses of StenB in TNBC cells and examine the drug safety in normal cells. StenB effectively suppressed the proliferation of TNBC cells rather than normal cells in terms of an ATP assay. This preferential antiproliferative function was alleviated by pretreating inhibitors for oxidative stress (N-acetylcysteine (NAC)) and apoptosis (Z-VAD-FMK). Accordingly, the oxidative-stress-related mechanisms were further assessed. StenB caused subG1 and G2/M accumulation but reduced the G1 phase in TNBC cells, while normal cells remained unchanged between the control and StenB treatments. The apoptosis behavior of TNBC cells was suppressed by StenB, whereas that of normal cells was not suppressed according to an annexin V assay. StenB-modulated apoptosis signaling, such as for caspases 3, 8, and 9, was more significantly activated in TNBC than in normal cells. StenB also caused oxidative stress in TNBC cells but not in normal cells according to a flow cytometry assay monitoring reactive oxygen species, mitochondrial superoxide, and their membrane potential. StenB induced greater DNA damage responses (γH2AX and 8-hydroxy-2-deoxyguanosine) in TNBC than in normal cells. All these StenB responses were alleviated by NAC pretreatment. Collectively, StenB modulated oxidative stress responses, leading to the antiproliferation of TNBC cells with little cytotoxicity in normal cells.
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- 2023
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29. Connection of Cancer Exosomal LncRNAs, Sponging miRNAs, and Exosomal Processing and Their Potential Modulation by Natural Products
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Ya-Ting Chuang, Jun-Ping Shiau, Jen-Yang Tang, Ammad Ahmad Farooqi, Fang-Rong Chang, Yi-Hong Tsai, Ching-Yu Yen, and Hsueh-Wei Chang
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exosome ,anticancer ,lncRNAs ,sponging miRNAs ,exosomal processing ,natural product ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Cancerous exosomes contain diverse biomolecules that regulate cancer progression. Modulating exosome biogenesis with clinical drugs has become an effective strategy for cancer therapy. Suppressing exosomal processing (assembly and secretion) may block exosomal function to reduce the proliferation of cancer cells. However, the information on natural products that modulate cancer exosomes lacks systemic organization, particularly for exosomal long noncoding RNAs (lncRNAs). There is a gap in the connection between exosomal lncRNAs and exosomal processing. This review introduces the database (LncTarD) to explore the potential of exosomal lncRNAs and their sponging miRNAs. The names of sponging miRNAs were transferred to the database (miRDB) for the target prediction of exosomal processing genes. Moreover, the impacts of lncRNAs, sponging miRNAs, and exosomal processing on the tumor microenvironment (TME) and natural-product-modulating anticancer effects were then retrieved and organized. This review sheds light on the functions of exosomal lncRNAs, sponging miRNAs, and exosomal processing in anticancer processes. It also provides future directions for the application of natural products when regulating cancerous exosomal lncRNAs.
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- 2023
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30. A retrospective study on sex difference in patients with urolithiasis: who is more vulnerable to chronic kidney disease?
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Tsu-Ming Chien, Yen-Man Lu, Ching-Chia Li, Wen-Jeng Wu, Hsueh-Wei Chang, and Yii-Her Chou
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Chronic kidney disease ,Urolithiasis ,Gender ,Medicine ,Physiology ,QP1-981 - Abstract
Abstract Background Urolithiasis is considered a vital public health issue with a substantial burden on kidney function. Additionally, only few reports focused on the gender difference in patients with urolithiasis. Therefore, this study aimed to compare the clinical characteristics of sex difference and their potential risk for chronic kidney disease (CKD) in patients with urolithiasis. Methods Patients diagnosed with stone disease from 2013 to 2018 were retrospectively reviewed and divided into two groups by gender. Clinical demographic characteristics, stone location, stone composition, urine chemistries, and renal function were investigated. Univariate and multivariate analyses were used to assess the relationship and potential risk of CKD between sex groups. Results A total of 1802 patients were included: 1312 from men and 490 from women. Female patients had a higher rate of hypertension, diabetes, and dyslipidemia. Male patients predominantly had calcium-containing stones, especially calcium oxalate stone, uric acid stone, and struvite stone. Carbonate apatite stone was more frequently found in women. Complex surgeries such as percutaneous nephrolithotomy (PCNL) and ureteroscopic lithotripsy (URSL) were more frequently performed in women than that in men. Multivariate analysis confirmed that age > 60 years (odds ratios [ORs] = 6.36; 95% confidence interval [CI], 3.8–10.8), female sex (ORs = 5.31; 95% CI 3.3–8.4), uric acid stone (ORs = 3.55; 95% CI 2.0–6.4), hypertension (OR = 7.20; 95% CI 3.8–13.7), and diabetes (OR = 7.06; 95% CI 3.1–16.2) were independent predictors of poor prognoses in CKD. Conclusions The female gender is significantly associated with a higher prevalence of CKD among patients with urolithiasis. Therefore, women with stone disease may need close renal function monitoring during follow-up.
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- 2021
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31. Riociguat treatment in patients with chronic thromboembolic pulmonary hypertension: Final safety data from the EXPERT registry
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Caneva, Jorge, Tuhay, Graciela, Diez, Mirta, Talavera, Maria Lujan, Acosta, Adriana, Vulcano, Norberto, Bosio, Martin, Maldonado, Lorena, Deleo, Sabino, Melatini, Luciano, Keogh, Anne, Kotlyar, Eugene, Feenstra, John, Dwyer, Nathan, Adams, Heath, Stevens, Wendy, Steele, Peter, Proudman, Susanna, Minson, Robert, Reeves, Glenn, Lavender, Melanie, Ng, Benjamin, Mackenzie, Michele, Barry, Lisa, Gruenberger, Margarethe, Huber, Charlotte, Lang, Irene, Tilea, Ioana, Sadushi-Kolici, Roela, Löffler-Ragg, Judith, Feistmantl, Lisa-Theresa, Evrard, Patrick, Louis, Renaud, Guiot, Julien, Naldi, Marco, De Pauw, Michel, Mehta, Sanjay, Camacho, Rafael Conde, Tovar, Patricia Parada, Londoño, Alejandro, Campo, Felipe, Garcia, Paula, Lema, Camila, Orozco-Levi, Mauricio, Martinez, William, Gomez, Juan Esteban, Nielsen-Kudsk, Jens Erik, Mellemkjaer, Soren, Anton, Ly, Altraja, Alan, Vihinen, Tapani, Vasankari, Tuija, Sitbon, Olivier, Cottin, Vincent, Têtu, Laurent, Noël-Savina, Elise, Shearman, Nicole, Tayler, Susanne, Olzik, Ilona, Kulka, Christine, Grimminger, Jan, Simon, Marcel, Nolde, Anna, Oqueka, Tim, Harbaum, Lars, Egenlauf, Benjamin, Ewert, Ralf, Schulz, Christian, Regotta, Sabine, Kramer, Tilmann, Knoop-Busch, Susanne, Gerhardt, Felix, Konstantinides, Stavros, Pitsiou, Georgia, Stanopoulos, Ioannis, Sourla, Evdokia, Mouratoglou, Sofia, Karvounis, Haralambos, Pappas, Athanasios, Georgopoulos, Dimitrios, Fanaridis, Michail, Mitrouska, Ioanna, Michalis, Lampros, Pappas, Konstantinos, Kotsia, Anna, Gaine, Sean, Vizza, Carmine Dario, Manzi, Giovanna, Poscia, Roberto, Badagliacca, Roberto, Agostoni, Piergiuseppe, Bruno, Noemi, Farina, Stefania, D'Alto, Michele, Argiento, Paola, Correra, Anna, Di Marco, Giovanni Maria, Cresci, Chiara, Vannucchi, Vieri, Torricelli, Elena, Garcea, Alessio, Pesci, Alberto, Sardella, Luca, Paciocco, Giuseppe, Pane, Federico, D'Armini, Andrea Maria, Pin, Maurizio, Grazioli, Valentina, Massola, Giulia, Sciortino, Antonio, Prediletto, Renato, Bauleo, Carolina, Airò, Edoardo, Ndreu, Rudina, Pavlickova, Ivana, Lunardi, Claudio, Mulè, Massimiliano, Farruggio, Silvia, Costa, Serena, Galgano, Giuseppe, Petruzzi, Mario, De Luca, Anna, Lombardi, Francesco, Roncon, Loris, Conte, Luca, Picariello, Claudio, Wirtz, Gil, Alexandre, Myriam, Vonk-Noordegraaf, A., Boogaard, H., Mager, J., Reesink, H., van den Toorn, Leon M., Boomars, Karin, Andreassen, Arne K., Castro, Graça, Tania, Gonçalves, Baptista, Rui, Marinho, António, Shiang, Teresa, Oliveira, Ana, Coutinho, Daniel, Sousa, Joana, Loureiro, Maria José, Repolho, Débora, Martins Jesus, Susana Maria, Capinha, Marta, Agostinho, João, Cardoso, Tania, Rocha, Andreia, Espinha, Mafalda, Ivanov, Kyundyul Ivanovich, Alexeeva, Dalyana Eduardovna, Batalina, Marina Vadimovna, Hegya, Daria Viktorovna, Zvereva, Tatyana Nikolaevna, Avdeev, Sergey Nikolaevich, Tsareva, Natalia Anatolievna, Galyavich, Albert Sarvatovich, Nikolaevich, Bykov Aleksander, Filippov, Evgeny Vladimirovich, Yakovleva, Olga Eduardovna, Pavlova, Olga Borisovna, Skripkina, Elena Sergeevna, Martynyuk, Tamila Vitalievna, Bukatova, Irina Fedorovna, Tregubova, Anna Viktorovna, Platonov, Dmitry Yurievich, Kolomeytseva, Tatyana Mikhaylovna, Al Dalaan, Abdullah, Abdelsayed, Abeer Abeer, Weheba, Ihab, Saleemi, Sarferaz, Sakkijha, Hussam, Bohacekova, Marcela, Valkovicova, Tatiana, Farkasova, Iveta, Quezada, Carlos Andres, Piccari, Lucilla, Blanco, Isabel, Sebastian, Laura, Roman, Antonio, Lopez, Manuel, Otero, Remedios, Elias, Teresa, Jara, Luis, Asencio, Isabel, Arjona, Josefa Jiménez, Almagro, Raúl Menor, Cárdenas, Salvador López, García, Salvador Alcaraz, Rodríguez, Patricia Villanueva, Lopez, Raquel, Garcia, Alberto, Avilés, Francisco Fernandez, De La Pava, Sebastian, Yotti, Raquel, Peñate, Gregorio Pérez, Marrero, Fernando León, Cifrián Martínez, José Manuel, Martinez-Meñaca, Amaya, Alonso, Lecue Pilar, Rozas, Sonia Fernandez, Fernandez, David Iturbe, Cuesta, Victor Mora, Söderberg, Stefan, Bartfay, Sven-Erik, Rundqvist, Bengt, Alfetlawi, Monthir, Wodlin, Peter, Schwarz, Esther Irene, Speich, Rudolf, Lador, Frédéric, Rochat, Thierry, Gasche-Soccal, Paola, Hsu, Chih-Hsin, Lin, Tsung-Hsien, Su, Ho-Ming, Lai, Wen-Ter, Chu, Chun Yuan, Hsu, Po-Chao, Voon, Wen-Chol, Yen, Hsueh-Wei, Yih-Jer Wu, Jacob, Wu, Shu-Hao, Huang, Wen-Pin, Fong, Man-Cai, Huang, Chien-Lung, Kuo, Ping-Hung, Lin, Yen-Hung, Lin, Jiunn-Lee, Hung, Chi-Sheng, Wu, Cho-Kai, Sung, Shih-Hsien, Huang, Wei-Chun, Cheng, Chin-Chang, Kuo, Shu-Hung, Wang, Wen-Hwa, Ho, Wan-Jing, Hsu, Tsu-Shiu, Mutlu, Bülent, Atas, Halil, Ongen, Gul, Un, Zeynep, Okumus, Gulfer, Hanta, Ismail, Corris, Paul, Peacock, Andrew, Church, Colin, Toshner, Mark, Newnham, Michael, Ghofrani, Hossein-Ardeschir, Gomez Sanchez, Miguel-Angel, Humbert, Marc, Pittrow, David, Simonneau, Gérald, Gall, Henning, Grünig, Ekkehard, Klose, Hans, Halank, Michael, Langleben, David, Snijder, Repke J., Escribano Subias, Pilar, Mielniczuk, Lisa M., Lange, Tobias J., Vachiéry, Jean-Luc, Wirtz, Hubert, Helmersen, Douglas S., Tsangaris, Iraklis, Barberá, Joan A., Pepke-Zaba, Joanna, Boonstra, Anco, Rosenkranz, Stephan, Ulrich, Silvia, Steringer-Mascherbauer, Regina, Delcroix, Marion, Jansa, Pavel, Šimková, Iveta, Giannakoulas, George, Klotsche, Jens, Williams, Evgenia, Meier, Christian, and Hoeper, Marius M.
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- 2021
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32. Riociguat treatment in patients with pulmonary arterial hypertension: Final safety data from the EXPERT registry
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Caneva, Jorge, Tuhay, Graciela, Diez, Mirta, Talavera, Maria Lujan, Acosta, Adriana, Vulcano, Norberto, Bosio, Martin, Maldonado, Lorena, Deleo, Sabino, Melatini, Luciano, Keogh, Anne, Kotlyar, Eugene, Feenstra, John, Dwyer, Nathan, Adams, Heath, Stevens, Wendy, Steele, Peter, Proudman, Susanna, Minson, Robert, Reeves, Glenn, Lavender, Melanie, Ng, Benjamin, Mackenzie, Michele, Barry, Lisa, Gruenberger, Margarethe, Huber, Charlotte, Lang, Irene, Tilea, Ioana, Sadushi-Kolici, Roela, Löffler-Ragg, Judith, Feistmantl, Lisa-Theresa, Evrard, Patrick, Guiot, Julien, Naldi, Marco, De Pauw, Michel, Louis, Renaud, Mehta, Sanjay, Camacho, Rafael Conde, Tovar, Patricia Parada, Londoño, Alejandro, Campo, Felipe, Garcia, Paula, Lema, Camila, Orozco-Levi, Mauricio, Martinez, William, Gomez, Juan Esteban, Nielsen-Kudsk, Jens Erik, Mellemkjaer, Soren, Anton, Ly, Altraja, Alan, Vihinen, Tapani, Vasankari, Tuija, Sitbon, Olivier, Cottin, Vincent, Têtu, Laurent, Noël-Savina, Elise, Shearman, Nicole, Tayler, Susanne, Olzik, Ilona, Kulka, Christine, Grimminger, Jan, Simon, Marcel, Nolde, Anna, Oqueka, Tim, Harbaum, Lars, Egenlauf, Benjamin, Ewert, Ralf, Schulz, Christian, Regotta, Sabine, Kramer, Tilmann, Knoop-Busch, Susanne, Gerhardt, Felix, Konstantinides, Stavros, Pitsiou, Georgia, Stanopoulos, Ioannis, Sourla, Evdokia, Mouratoglou, Sofia, Karvounis, Haralambos, Pappas, Athanasios, Mitrouska, Ioanna, Georgopoulos, Dimitrios, Fanaridis, Michail, Michalis, Lampros, Pappas, Konstantinos, Kotsia, Anna, Gaine, Sean, Vizza, Carmine Dario, Manzi, Giovanna, Poscia, Roberto, Badagliacca, Roberto, Agostoni, Piergiuseppe, Bruno, Noemi, Farina, Stefania, D'Alto, Michele, Argiento, Paola, Correra, Anna, Di Marco, Giovanni Maria, Cresci, Chiara, Vannucchi, Vieri, Torricelli, Elena, Garcea, Alessio, Pesci, Alberto, Sardella, Luca, Paciocco, Giuseppe, Pane, Federico, D'Armini, Andrea Maria, Pin, Maurizio, Grazioli, Valentina, Massola, Giulia, Sciortino, Antonio, Prediletto, Renato, Bauleo, Carolina, Airò, Edoardo, Ndreu, Rudina, Pavlickova, Ivana, Lunardi, Claudio, Farruggio, Silvia, Costa, Serena, Mulè, Massimiliano, Galgano, Giuseppe, Petruzzi, Mario, De Luca, Anna, Lombardi, Francesco, Roncon, Loris, Conte, Luca, Picariello, Claudio, Wirtz, Gil, Alexandre, Myriam, Vonk-Noordegraaf, A., Boogaard, H., Mager, J., Reesink, H., van den Toorn, Leon M., Boomars, Karin, Andreassen, Arne K., Castro, Graça, Tania, Gonçalves, Baptista, Rui, Marinho, António, Shiang, Teresa, Oliveira, Ana, Coutinho, Daniel, Sousa, Joana, Loureiro, Maria José, Repolho, Débora, Martins Jesus, Susana Maria, Capinha, Marta, Agostinho, João, Cardoso, Tania, Rocha, Andreia, Espinha, Mafalda, Ivanov, Kyundyul Ivanovich, Alexeeva, Dalyana Eduardovna, Batalina, Marina Vadimovna, Hegya, Daria Viktorovna, Zvereva, Tatyana Nikolaevna, Avdeev, Sergey Nikolaevich, Tsareva, Natalia Anatolievna, Galyavich, Albert Sarvatovich, Nikolaevich, Bykov Aleksander, Filippov, Evgeny Vladimirovich, Yakovleva, Olga Eduardovna, Pavlova, Olga Borisovna, Skripkina, Elena Sergeevna, Martynyuk, Tamila Vitalievna, Bukatova, Irina Fedorovna, Tregubova, Anna Viktorovna, Platonov, Dmitry Yurievich, Kolomeytseva, Tatyana Mikhaylovna, Al Dalaan, Abdullah, Abdelsayed, Abeer Abeer, Weheba, Ihab, Saleemi, Sarferaz, Sakkijha, Hussam, Bohacekova, Marcela, Valkovicova, Tatiana, Farkasova, Iveta, Quezada, Carlos Andres, Piccari, Lucilla, Blanco, Isabel, Sebastian, Laura, Roman, Antonio, Lopez, Manuel, Otero, Remedios, Elias, Teresa, Jara, Luis, Asencio, Isabel, Arjona, Josefa Jiménez, Almagro, Raúl Menor, Cárdenas, Salvador López, García, Salvador Alcaraz, Rodríguez, Patricia Villanueva, Lopez, Raquel, Garcia, Alberto, Avilés, Francisco Fernandez, De La Pava, Sebastian, Yotti, Raquel, Peñate, Gregorio Pérez, Marrero, Fernando León, Cifrián Martínez, José Manuel, Martinez-Meñaca, Amaya, Alonso, Lecue Pilar, Rozas, Sonia Fernandez, Fernandez, David Iturbe, Cuesta, Victor Mora, Söderberg, Stefan, Bartfay, Sven-Erik, Rundqvist, Bengt, Alfetlawi, Monthir, Wodlin, Peter, Schwarz, Esther Irene, Speich, Rudolf, Lador, Frédéric, Rochat, Thierry, Gasche-Soccal, Paola, Hsu, Chih-Hsin, Lin, Tsung-Hsien, Su, Ho-Ming, Lai, Wen-Ter, Chu, Chun Yuan, Hsu, Po-Chao, Voon, Wen-Chol, Yen, Hsueh-Wei, Yih-Jer Wu, Jacob, Wu, Shu-Hao, Huang, Wen-Pin, Fong, Man-Cai, Huang, Chien-Lung, Kuo, Ping-Hung, Lin, Yen-Hung, Lin, Jiunn-Lee, Hung, Chi-Sheng, Wu, Cho-Kai, Sung, Shih-Hsien, Huang, Wei-Chun, Cheng, Chin-Chang, Kuo, Shu-Hung, Wang, Wen-Hwa, Ho, Wan-Jing, Hsu, Tsu-Shiu, Mutlu, Bülent, Atas, Halil, Ongen, Gul, Un, Zeynep, Okumus, Gulfer, Hanta, Ismail, Corris, Paul, Peacock, Andrew, Church, Colin, Toshner, Mark, Newnham, Michael, Hoeper, Marius M., Gomez Sanchez, Miguel-Angel, Humbert, Marc, Pittrow, David, Simonneau, Gérald, Gall, Henning, Grünig, Ekkehard, Klose, Hans, Halank, Michael, Langleben, David, Snijder, Repke J., Escribano Subias, Pilar, Mielniczuk, Lisa M., Lange, Tobias J., Vachiéry, Jean-Luc, Wirtz, Hubert, Helmersen, Douglas S., Tsangaris, Iraklis, Barberà, Joan A., Pepke-Zaba, Joanna, Boonstra, Anco, Rosenkranz, Stephan, Ulrich, Silvia, Steringer-Mascherbauer, Regina, Delcroix, Marion, Jansa, Pavel, Šimková, Iveta, Giannakoulas, George, Klotsche, Jens, Williams, Evgenia, Meier, Christian, and Ghofrani, Hossein-Ardeschir
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- 2021
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33. Theoretical analysis of vibration characteristics of rectangular thin plate fully immersed in fluid with finite dimension
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Liao, Chan-Yi, Chen, Guan-Wei, Hsu, Hsueh-Wei, and Ma, Chien-Ching
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- 2021
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34. VoPiFNet: Voxel-Pixel Fusion Network for Multi-Class 3D Object Detection
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Wang, Chia-Hung, Chen, Hsueh-Wei, Chen, Yi, Hsiao, Pei-Yung, and Fu, Li-Chen
- Abstract
Many LiDAR-based methods for detecting large objects, single-class object detection, or under easy situations were claimed to perform well. However, due to their failure to exploit image semantics, their performance in detecting small targets or under challenging conditions does not exceed that of fusion-based approaches. In order to elevate the detection performance in a complex environment, this paper proposes a multi-modal and multi-class 3D object detection network, named Voxel-Pixel Fusion Network (VoPiFNet). Within this network, we design a key novel component called the Voxel-Pixel Fusion Layer, which takes advantage of the geometric relation of a voxel-pixel pair and effectively fuses voxel features and pixel features with the cross-modal attention mechanism. Moreover, after considering the characteristics of the voxel-pixel pair, we design four parameters to guide and enhance this fusion effect. This proposed layer can be integrated with voxel-based 3D LiDAR detectors and 2D image detectors. Finally, the proposed method is evaluated on the public KITTI benchmark dataset for multi-class 3D object detection at different levels. Extensive experiments show that our method outperforms the state-of-the-art methods in detecting challenging pedestrian category and achieve promising performance in overall 3D mean average precision (mAP).
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- 2024
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35. Single-crystalline aluminum film for ultraviolet plasmonic nanolasers
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Chou, Bo-Tsun, Chou, Yu-Hsun, Wu, Yen-Mo, Chung, Yi-Chen, Hsueh, Wei-Jen, Lin, Shih-Wei, Lu, Tien-Chang, Lin, Tzy-Rong, and Lin, Sheng-Di
- Subjects
Condensed Matter - Mesoscale and Nanoscale Physics ,Physics - Optics - Abstract
Plasmonic devices have advanced significantly in the past decade. Being one of the most intriguing devices, plamonic nanolasers plays an important role in biomedicine, chemical sensor, information technology, and optical integrated circuits. However, nanoscale plasmonic devices, particularly in ultraviolet regime, are extremely sensitive to metal and interface quality, which renders the development of ultraviolet plasmonics. Here, by addressing the material issues, we demonstrate a low threshold, high characteristic temperature metal-oxide-semiconductor ZnO nanolaser working at room temperature. The template for ZnO nanowires consists of a flat single-crystalline aluminum film grown by molecular beam epitaxy and an ultra-smooth Al2O3 spacer layer prepared by atomic layer deposition. By effectively reducing surface plasmon scattering loss and metal intrinsic absorption loss, the high-quality metal film and sharp interfaces between layers boost the device performance. Our work paves the way for future applications using ultraviolet plasmonic nanolasers and related devices., Comment: 19 pages, 6 figures
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- 2015
36. Comparison between estimated and brachial‐ankle pulse wave velocity for cardiovascular and overall mortality prediction
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Po‐Chao Hsu, Wen‐Hsien Lee, Wei‐Chung Tsai, Ying‐Chih Chen, Chun‐Yuan Chu, Hsueh‐Wei Yen, Tsung‐Hsien Lin, Wen‐Chol Voon, Wen‐Ter Lai, Sheng‐Hsiung Sheu, Ho‐Ming Su, and Cheng‐An Chiu
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brachial‐ankle pulse wave velocity ,estimated pulse wave velocity ,mortality ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Pulse wave velocity (PWV) was a good marker of arterial stiffness and could predict cardiovascular (CV) outcomes. Recently, estimated PWV (ePWV) calculated by equations using age and mean blood pressure was reported to be an independent predictor of major CV events. However, there was no study comparing ePWV with brachial‐ankle PWV (baPWV) for CV and overall mortality prediction. We included 881 patients arranged for echocardiographic examination. BaPWV and blood pressures were measured by ankle‐brachial index‐form device. The median follow‐up period to mortality was 94 months. Mortality events were documented during the follow‐up period, including CV mortality (n = 66) and overall mortality (n = 184). Both of ePWV and baPWV were associated with increased CV and overall mortality after the multivariable analysis. ePWV had better predictive value than Framingham risk score (FRS) for CV and overall mortality prediction, but baPWV did not. In direct comparison of multivariable analysis using FRS as basic model, ePWV had a superior additive predictive value for CV mortality than baPWV (p = .030), but similar predictive valve for overall mortality as baPWV (p = .540). In conclusion, both ePWV and baPWV were independent predictors for long‐term CV and overall mortality in univariable and multivariable analysis. Besides, ePWV had a better additive predictive value for CV mortality than baPWV and similar predictive value for overall mortality as baPWV. Therefore, ePWV obtained without equipment deserved to be calculated for overall mortality prediction and better CV survival prediction.
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- 2021
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37. Synthesis and Anticancer Evaluation of 4-Anilinoquinolinylchalcone Derivatives
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Cheng-Yao Yang, Min-Yu Lee, Yeh-Long Chen, Jun-Ping Shiau, Yung-Hsiang Tsai, Chia-Ning Yang, Hsueh-Wei Chang, and Chih-Hua Tseng
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4-anilinoquinolinylchalcone ,lapatinib ,cytotoxicity ,reactive oxygen species ,apoptosis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
A series of 4-anilinoquinolinylchalcone derivatives were synthesized and evaluated for antiproliferative activities against the growth of human cancer cell lines (Huh-7 and MDA-MB-231) and normal lung cells (MRC-5). The results exhibited low cytotoxicity against human lung cells (MRC-5). Among them, (E)-3-{4-{[4-(benzyloxy)phenyl]amino}quinolin-2-yl}-1-(4-methoxyphenyl) prop-2-en-1-one (4a) was found to have the highest cytotoxicity in breast cancer cells and low cytotoxicity in normal cells. Compound 4a causes ATP depletion and apoptosis of breast cancer MDA-MB-231 cells and triggers reactive oxygen species (ROS)-dependent caspase 3/7 activation. In conclusion, it is worth studying 4-anilinoquinolinylchalcone derivatives further as new potential anticancer agents for the treatment of human cancers.
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- 2023
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38. Ginger-Derived 3HDT Exerts Antiproliferative Effects on Breast Cancer Cells by Apoptosis and DNA Damage
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Chung-Yi Chen, Yan-Ning Chen, Jun-Ping Shiau, Jen-Yang Tang, Ming-Feng Hou, and Hsueh-Wei Chang
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ginger ,TNBC ,DNA damage ,apoptosis ,oxidative stress ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Ginger-derived compounds are abundant sources of anticancer natural products. However, the anticancer effects of (E)-3-hydroxy-1-(4′-hydroxy-3′,5′-dimethoxyphenyl)-tetradecan-6-en-5-one (3HDT) have not been examined. This study aims to assess the antiproliferation ability of 3HDT on triple-negative breast cancer (TNBC) cells. 3HDT showed dose-responsive antiproliferation for TNBC cells (HCC1937 and Hs578T). Moreover, 3HDT exerted higher antiproliferation and apoptosis on TNBC cells than on normal cells (H184B5F5/M10). By examining reactive oxygen species, mitochondrial membrane potential, and glutathione, we found that 3HDT provided higher inductions for oxidative stress in TNBC cells compared with normal cells. Antiproliferation, oxidative stress, antioxidant signaling, and apoptosis were recovered by N-acetylcysteine, indicating that 3HDT preferentially induced oxidative-stress-mediated antiproliferation in TNBC cells but not in normal cells. Moreover, by examining γH2A histone family member X (γH2AX) and 8-hydroxy-2-deoxyguanosine, we found that 3HDT provided higher inductions for DNA damage, which was also reverted by N-acetylcysteine. In conclusion, 3HDT is an effective anticancer drug with preferential antiproliferation, oxidative stress, apoptosis, and DNA damage effects on TNBC cells.
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- 2023
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39. Modulation of AKT Pathway-Targeting miRNAs for Cancer Cell Treatment with Natural Products
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Jun-Ping Shiau, Ya-Ting Chuang, Ching-Yu Yen, Fang-Rong Chang, Kun-Han Yang, Ming-Feng Hou, Jen-Yang Tang, and Hsueh-Wei Chang
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AKT ,miRNA ,bioactive substance ,natural products ,cell function ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Many miRNAs are known to target the AKT serine-threonine kinase (AKT) pathway, which is critical for the regulation of several cell functions in cancer cell development. Many natural products exhibiting anticancer effects have been reported, but their connections to the AKT pathway (AKT and its effectors) and miRNAs have rarely been investigated. This review aimed to demarcate the relationship between miRNAs and the AKT pathway during the regulation of cancer cell functions by natural products. Identifying the connections between miRNAs and the AKT pathway and between miRNAs and natural products made it possible to establish an miRNA/AKT/natural product axis to facilitate a better understanding of their anticancer mechanisms. Moreover, the miRNA database (miRDB) was used to retrieve more AKT pathway-related target candidates for miRNAs. By evaluating the reported facts, the cell functions of these database-generated candidates were connected to natural products. Therefore, this review provides a comprehensive overview of the natural product/miRNA/AKT pathway in the modulation of cancer cell development.
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- 2023
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40. Oxidative-Stress-Mediated ER Stress Is Involved in Regulating Manoalide-Induced Antiproliferation in Oral Cancer Cells
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Sheng-Yao Peng, Jen-Yang Tang, Ting-Hsun Lan, Jun-Ping Shiau, Kuan-Liang Chen, Jiiang-Huei Jeng, Ching-Yu Yen, and Hsueh-Wei Chang
- Subjects
marine sponges ,ER stress ,ER expansion ,aggresome ,apoptosis ,autophagy ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Manoalide provides preferential antiproliferation of oral cancer but is non-cytotoxic to normal cells by modulating reactive oxygen species (ROS) and apoptosis. Although ROS interplays with endoplasmic reticulum (ER) stress and apoptosis, the influence of ER stress on manoalide-triggered apoptosis has not been reported. The role of ER stress in manoalide-induced preferential antiproliferation and apoptosis was assessed in this study. Manoalide induces a higher ER expansion and aggresome accumulation of oral cancer than normal cells. Generally, manoalide differentially influences higher mRNA and protein expressions of ER-stress-associated genes (PERK, IRE1α, ATF6, and BIP) in oral cancer cells than in normal cells. Subsequently, the contribution of ER stress on manoalide-treated oral cancer cells was further examined. ER stress inducer, thapsigargin, enhances the manoalide-induced antiproliferation, caspase 3/7 activation, and autophagy of oral cancer cells rather than normal cells. Moreover, N-acetylcysteine, an ROS inhibitor, reverses the responses of ER stress, aggresome formation, and the antiproliferation of oral cancer cells. Consequently, the preferential ER stress of manoalide-treated oral cancer cells is crucial for its antiproliferative effect.
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- 2023
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41. Modulating Effects of Cancer-Derived Exosomal miRNAs and Exosomal Processing by Natural Products
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Ya-Ting Chuang, Jen-Yang Tang, Jun-Ping Shiau, Ching-Yu Yen, Fang-Rong Chang, Kun-Han Yang, Ming-Feng Hou, Ammad Ahmad Farooqi, and Hsueh-Wei Chang
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exosome ,miRNA ,cell function ,natural product ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Cancer-derived exosomes exhibit sophisticated functions, such as proliferation, apoptosis, migration, resistance, and tumor microenvironment changes. Several clinical drugs modulate these exosome functions, but the impacts of natural products are not well understood. Exosome functions are regulated by exosome processing, such as secretion and assembly. The modulation of these exosome-processing genes can exert the anticancer and precancer effects of cancer-derived exosomes. This review focuses on the cancer-derived exosomal miRNAs that regulate exosome processing, acting on the natural-product-modulating cell functions of cancer cells. However, the role of exosomal processing has been overlooked in several studies of exosomal miRNAs and natural products. In this study, utilizing the bioinformatics database (miRDB), the exosome-processing genes of natural-product-modulated exosomal miRNAs were predicted. Consequently, several natural drugs that modulate exosome processing and exosomal miRNAs and regulate cancer cell functions are described here. This review sheds light on and improves our understanding of the modulating effects of exosomal miRNAs and their potential exosomal processing targets on anticancer treatments based on the use of natural products.
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- 2023
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42. Extract of pre-germinated brown rice protects against cardiovascular dysfunction by reducing levels of inflammation and free radicals in a rat model of type II diabetes
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Liang, Hsin-Li, Cheng, Pei-Wen, Lin, Hui-Li, Hao, Chi-Long, Ke, Liang-Yin, Chou, Huei-Yin, Tseng, Yu-Hsiu, Yen, Hsueh-Wei, and Shen, Kuo-Ping
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- 2020
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43. Ferroptosis-Regulated Natural Products and miRNAs and Their Potential Targeting to Ferroptosis and Exosome Biogenesis.
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Chuang, Ya-Ting, Yen, Ching-Yu, Chien, Tsu-Ming, Chang, Fang-Rong, Tsai, Yi-Hong, Wu, Kuo-Chuan, Tang, Jen-Yang, and Chang, Hsueh-Wei
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NATURAL products ,EXOSOMES ,MICRORNA ,EXTRACELLULAR vesicles ,TREATMENT effectiveness - Abstract
Ferroptosis, which comprises iron-dependent cell death, is crucial in cancer and non-cancer treatments. Exosomes, the extracellular vesicles, may deliver biomolecules to regulate disease progression. The interplay between ferroptosis and exosomes may modulate cancer development but is rarely investigated in natural product treatments and their modulating miRNAs. This review focuses on the ferroptosis-modulating effects of natural products and miRNAs concerning their participation in ferroptosis and exosome biogenesis (secretion and assembly)-related targets in cancer and non-cancer cells. Natural products and miRNAs with ferroptosis-modulating effects were retrieved and organized. Next, a literature search established the connection of a panel of ferroptosis-modulating genes to these ferroptosis-associated natural products. Moreover, ferroptosis-associated miRNAs were inputted into the miRNA database (miRDB) to bioinformatically search the potential targets for the modulation of ferroptosis and exosome biogenesis. Finally, the literature search provided a connection between ferroptosis-modulating miRNAs and natural products. Consequently, the connections from ferroptosis–miRNA–exosome biogenesis to natural product-based anticancer treatments are well-organized. This review sheds light on the research directions for integrating miRNAs and exosome biogenesis into the ferroptosis-modulating therapeutic effects of natural products on cancer and non-cancer diseases. [ABSTRACT FROM AUTHOR]
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- 2024
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44. Synthesis of bioactive evodiamine and rutaecarpine analogues under ball milling conditions.
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Hu, Hao-Chun, Yu, Szu-Yin, Tsai, Yi-Hong, Hsieh, Pei-Wen, Wang, Hui-Chun, Chen, Yan-Ning, Chuang, Ya-Ting, Lee, Min-Yu, Chang, Hsueh-Wei, Wu, Yang-Chang, Chang, Fang-Rong, Szatmári, István, and Fülöp, Ferenc
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- 2024
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45. Comparison of different ankle-brachial indices in the prediction of overall and cardiovascular mortality
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Hsu, Po-Chao, Lee, Wen-Hsien, Chen, Ying-Chih, Lee, Meng-Kuang, Tsai, Wei-Chung, Chu, Chun-Yuan, Lee, Chee-Siong, Yen, Hsueh-Wei, Lin, Tsung-Hsien, Voon, Wen-Chol, Lai, Wen-Ter, Sheu, Sheng-Hsiung, and Su, Ho-Ming
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- 2020
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46. Chemical Constituents from Soft Coral Clavularia spp. Demonstrate Antiproliferative Effects on Oral Cancer Cells
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Cheng, Ming-Ya, primary, Chuang, Ya-Ting, additional, Chang, Hsueh-Wei, additional, Lin, Zheng-Yu, additional, Chen, Ching-Yeu, additional, and Cheng, Yuan-Bin, additional
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- 2023
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47. Intrinsic tryptophan fluorescence quenching by iodine in non-canonical amino acid reveals alteration of the hydrogen bond network in the photoactive orange carotenoid protein
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Tsoraev, Georgy V., primary, Bukhanko, Antonina Y., additional, Mamchur, Alexandra A., additional, Yaroshevich, Igor A., additional, Sluchanko, Nikolai N., additional, Tseng, Hsueh-Wei, additional, Moldenhauer, Marcus, additional, Budisa, Nediljko, additional, Friedrich, Thomas, additional, and Maksimov, Eugene G., additional
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- 2023
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48. Flexibanone, the first cembranoid possessing an α-methylene-δ-lactone linking with a tetrahydronfuran ring by C-3/C-4 single bond, from the soft coral Sinularia flexibilis
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Tai, Chi-Jen, primary, Zhang, Hong-Wei, additional, Wang, Hui-Chun, additional, Chuang, Ya-Ting, additional, Chang, Hsueh-Wei, additional, Chang, Fang-Rong, additional, Sung, Ping-Jyun, additional, and Sheu, Jyh-Horng, additional
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- 2023
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49. GLPose: Global-Local Attention Network with Feature Interpolation Regularization for Head Pose Estimation of People Wearing Facial Masks.
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Hsueh-Wei Chen, Yi Chen, Pei-Yung Hsiao, Li-Chen Fu, and Zirong Ding
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- 2022
50. A retrospective study on sex difference in patients with urolithiasis: who is more vulnerable to chronic kidney disease?
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Chien, Tsu-Ming, Lu, Yen-Man, Li, Ching-Chia, Wu, Wen-Jeng, Chang, Hsueh-Wei, and Chou, Yii-Her
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- 2021
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