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2. The ACR11 encodes a novel type of chloroplastic ACT domain repeat protein that is coordinately expressed with GLN2 in Arabidopsis

Author

Hsu Chih-Ping, Chung Tsui-Yun, Sung Tzu-Ying, and Hsieh Ming-Hsiun

Subjects
Botany, QK1-989
Abstract

Abstract Background The ACT domain, named after bacterial aspartate kinase, chorismate mutase and TyrA (prephenate dehydrogenase), is a regulatory domain that serves as an amino acid-binding site in feedback-regulated amino acid metabolic enzymes. We have previously identified a novel type of ACT domain-containing protein family, the ACT domain repeat (ACR) protein family, in Arabidopsis. Members of the ACR family, ACR1 to ACR8, contain four copies of the ACT domain that extend throughout the entire polypeptide. Here, we describe the identification of four novel ACT domain-containing proteins, namely ACR9 to ACR12, in Arabidopsis. The ACR9 and ACR10 proteins contain three copies of the ACT domain, whereas the ACR11 and ACR12 proteins have a putative transit peptide followed by two copies of the ACT domain. The functions of these plant ACR proteins are largely unknown. Results The ACR11 and ACR12 proteins are predicted to target to chloroplasts. We used protoplast transient expression assay to demonstrate that the Arabidopsis ACR11- and ACR12-green fluorescent fusion proteins are localized to the chloroplast. Analysis of an ACR11 promoter-β-glucuronidase (GUS) fusion in transgenic Arabidopsis revealed that the GUS activity was mainly detected in mature leaves and sepals. Interestingly, coexpression analysis revealed that the GLN2, which encodes a chloroplastic glutamine synthetase, has the highest mutual rank in the coexpressed gene network connected to ACR11. We used RNA gel blot analysis to confirm that the expression pattern of ACR11 is similar to that of GLN2 in various organs from 6-week-old Arabidopsis. Moreover, the expression of ACR11 and GLN2 is highly co-regulated by sucrose and light/dark treatments in 2-week-old Arabidopsis seedlings. Conclusions This study reports the identification of four novel ACT domain repeat proteins, ACR9 to ACR12, in Arabidopsis. The ACR11 and ACR12 proteins are localized to the chloroplast, and the expression of ACR11 and GLN2 is highly coordinated. These results suggest that the ACR11 and GLN2 genes may belong to the same functional module. The Arabidopsis ACR11 protein may function as a regulatory protein that is related to glutamine metabolism or signaling in the chloroplast.

Published
2011
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4. Tocilizumab Exerts Anti-tumor Effects on Proliferation, Invasiveness, ERK 1/2 and STAT3 Signaling Transduction in Colorectal Carcinoma Cell Xenografts Corresponding to Expression Levels of IL-6 Receptors

Author

Chung, Yuan-Chiang, primary, Chen, Szu-Jung, additional, Chung, Yuan-Chiang, additional, Liu, Wei-Chun, additional, Lai, Ming-Tsung, additional, Kao, Ting-Yu, additional, Yang, Wei-Shun, additional, Yang, Chien-Hui, additional, Hsu, Chih-Ping, additional, and Chang, Jia-Feng, additional

Published
2023
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6. A Fc-VEGF chimeric fusion enhances PD-L1 immunotherapy via inducing immune reprogramming and infiltration in the immunosuppressive tumor microenvironment

Author

Kuo, Cheng-Liang, primary, Chou, Han-Yu, additional, Lien, Hui-Wen, additional, Yeh, Chia-An, additional, Wang, Jing-Rong, additional, Chen, Chung-Hsing, additional, Fan, Chi-Chen, additional, Hsu, Chih-Ping, additional, Kao, Ting-Yu, additional, Ko, Tai-Ming, additional, and Lee, Alan Yueh-Luen, additional

Published
2022
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7. Translational Medicine in Uremic Vascular Calcification: Scavenging ROS Attenuates p-Cresyl Sulfate-Activated Caspase-1, NLRP3 Inflammasome and Eicosanoid Inflammation in Human Arterial Smooth Muscle Cells

Author

Chang, Jia-Feng, primary, Kuo, Hsiao-Ling, additional, Liu, Shih-Hao, additional, Hsieh, Chih-Yu, additional, Hsu, Chih-Ping, additional, Hung, Kuo-Chin, additional, Wang, Ting-Ming, additional, Wu, Chang-Chin, additional, Lu, Kuo-Cheng, additional, Lin, Wei-Ning, additional, Hung, Chi-Feng, additional, and Ko, Wen-Chin, additional

Published
2022
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10. Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids

Author

Chung, Yuan-Chiang, Chiang, Hua-Che, Chang, Hsiang, Lin, Chih-Cheng, Lo, Li-Tsai, Wang, Ai-Yih, Chou, Kuo-Feng, and Hsu, Chih-Ping

Subjects
Apoptosis -- Research, Cancer cells -- Analysis, Soapberries (Sapindaceae) -- Physiological aspects -- Properties -- Research, Colorectal cancer -- Models -- Research, Health
Abstract

Byline: Yuan-Chiang. Chung, Hua-Che. Chiang, Hsiang. Chang, Chih-Cheng. Lin, Li-Tsai. Lo, Ai-Yih. Wang, Kuo-Feng. Chou, Chih-Ping. Hsu Aim of Study: Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to [...]

Published
2018

11. Research update for articles published in EJCI in 2010

Author

Adlbrecht, Christopher, Aigner, Elmar, Bellón, Juan M., Bouloukaki, Izolde, Bouzas-Mosquera, Alberto, Carrilho, Alexandre J. F., Chang, Kuo-Chu, Chattipakorn, Nipon, Chattipakorn, Siriporn C., Chen, Yi-Jen, Chung, Yuan-Chiang, Colah, Roshan, Datz, Christian, Frkjær, Jens B., Fujimori, Shunji, Georgiadou, Panagiota, Grion, Cintia M., Hsu, Chih-Ping, Hülsmann, Martin, Hung, Ming-Jui, Hung, Ming-Yow, Iliodromitis, Efstathios K., Lang, Irene M., Lee, Ting- I., März, Winfried, Nair, Sona B., Pascual, Gemma, Peteiro, Jesús, Sakamoto, Choitsu, Satomura, Atsushi, Schiza, Sophia E., Stärkel, Peter, Stojakovic, Tatjana, Vesely, David L., Walters, Darren L., and Yilmaz, Yusuf

Published
2012
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15. Association of overexpressed proline-directed protein kinase F(sub A) with chemoresistance, invasion, and recurrence in patients with bladder carcinoma

Author

Hsueh, Sheng-Fen, Lai, Ming-Tsung, Yang, Chuan-Ching, Chung, Yuan-Chiang, Hsu, Chih-Ping, Peng, Chien-Chung, Fu, Hsiao-Hui, Cheng, Yung-Ming, Chang, King-Jen, and Yang, Shiaw-Der

Subjects
Bladder cancer -- Patient outcomes, Bladder cancer -- Prognosis, Bladder cancer -- Physiological aspects, Protein kinases -- Health aspects, Health
Published
2002

18. Synergistic Effect of Combined Treatment with Longan Flower Extract and 5-Fluorouracil on Colorectal Cancer Cells.

Author

Chen, Szu-Jung, Chung, Yuan-Chiang, Chang, Han-Lung, Chang, Hsin-Ping, Chou, Jui-Ling, Lin, Chih-Cheng, Chen, Chih-Hsien, and Hsu, Chih-Ping

Subjects
CELL proliferation, APOPTOSIS, CELL cycle, CELL lines, CELL physiology, COMBINATION drug therapy, COLON tumors, DRUG synergism, FLOWERS, FLUOROURACIL, GENE expression, IMMUNOBLOTTING, MEDICINAL plants, BIOLOGICAL membranes, MESSENGER RNA, POLYMERASE chain reaction, RECTUM tumors, PLANT extracts, CELL survival, PHARMACODYNAMICS
Abstract

To investigate the influence of longan flower extract (LFE) on the sensitization of colorectal cancer (CRC) cells to 5-fluorouracil (5-FU) treatment, HT-29, Colo 320DM and SW480 cells were treated with LFE and 5-FU alone and in combination, and the cell viability was then assessed by trypan blue exclusion, the cell cycle by propidium iodide staining, the mitochondria membrane potential by rhodamine 123 staining, and the expression levels of associated genes by immunoblotting and quantitative real-time polymerase chain reaction. LFE and 5-FU synergistically inhibited cell proliferation of HT-29 and Colo 320DM cells. Combined treatment also elevated the level of loss of mitochondria membrane potential of these two CRC cells and arrested HT-29 cells in the S phase of the cell cycle, in association with down-regulation of cyclin A mRNA expression. LFE synergistically potentiated chemosensitivity to 5-FU in at least two CRC cell lines. The results indicated that LFE has potential as a novel agent for the sensitization of CRC cells to 5-FU. [ABSTRACT FROM AUTHOR]

Published
2020
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21. Induction of apoptosis and cell cycle arrest in human colorectal carcinoma by Litchi seed extract

Author

Hsu, Chih-Ping, Lin, Chih-Cheng, Huang, Chiu-Chen, Lin, Yi-Hsien, Chou, Jyh-Ching, Tsia, Yu-Ting, Su, Jhih-Rou, and Chung, Yuan-Chiang

Subjects
Materia medica, Vegetable -- Health aspects, Cell cycle -- Research, Colorectal cancer -- Physiological aspects, Apoptosis -- Research, Plant extracts -- Health aspects, Soapberries -- Health aspects, Biotechnology industry, High technology industry, Physiological aspects, Research, Health aspects
Abstract

The Litchi (Litchi chinensis) fruit products possess rich amounts of flavanoids and proanthocyanidins. Its pericarp has been shown to inhibit breast and liver cancer cell growth. However, the anticolorectal cancer effect of Litchi seed extract has not yet been reported. In this study, the effects of polyphenol-rich Litchi seed ethanol extract (LCSP) on the proliferation, cell cycle, and apoptosis of two colorectal cancer cell lines Colo320DM and SW480 were examined. The results demonstrated that LCSP significantly induced apoptotic cell death in a dose-dependent manner and arrested cell cycle in G2/M in colorectal carcinoma cells. LCSP also suppressed cyclins and elevated the Bax: Bcl-2 ratio and caspase 3 activity. This study provides in vitro evidence that LCSP serves as a potential chemopreventive agent for colorectal cancer., 1. Introduction Colorectal cancer (CRC) has become the most common type of cancer in Taiwan since the Taiwanese diet has become increasingly westernized during recent decades [1, 2]. Although Taiwan [...]

Published
2012
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24. Inhibitory Effect and Mechanisms of an Anthocyanins- and Anthocyanidins-Rich Extract from Purple-Shoot Tea on Colorectal Carcinoma Cell Proliferation

Author

Hsu, Chih-Ping, Shih, Yi-Ting, Lin, Bor-Ru, Chiu, Chui-Feng, and Lin, Chih-Cheng

Abstract

One newly bred variety of tea cultivar, purple-shoot tea, was selected to evaluate its antiproliferative effects on colorectal carcinoma cells, as well as normal colon cells. The phytochemicals and identified catechins of purple-shoot tea extract (PTE) were significantly higher than that of ordinary tea, especially the anthocyanins (surpassed by 135-fold) and anthocyanidins (surpassed by 3.5-fold). PTE inhibited the proliferation of COLO 320DM (IC50= 64.9 μg/mL) and HT-29 (IC50= 55.2 μg/mL) by blocking cell cycle progression during the G0/G1phase and inducing apoptotic death. Western blotting indicated that PTE induced cell cycle arrest by reducing the expression of cyclin E and cyclin D1 in COLO 320DM and the upregulation of p21 and p27 cyclin-dependent kinase inhibitors in HT-29. Two cells treated with PTE also indicated the cleavage of PARP, activation of caspase 3, and an increased Bax/Bcl-2 ratio. Our results showed that PTE is a potential novel dietary agent for colorectal cancer chemoprevention.

Published
2024
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26. Research update for articles published in EJCI in 2010

Author

Adlbrecht, Christopher Aigner, Elmar Bellon, Juan M. and Bouloukaki, Izolde Bouzas-Mosquera, Alberto Carrilho, Alexandre J. F. Chang, Kuo-Chu Chattipakorn, Nipon Chattipakorn, Siriporn C. Chen, Yi-Jen Chung, Yuan-Chiang Colah, Roshan and Datz, Christian Frokjaer, Jens B. Fujimori, Shunji and Georgiadou, Panagiota Grion, Cintia M. Hsu, Chih-Ping and Huelsmann, Martin Hung, Ming-Jui Hung, Ming-Yow and Iliodromitis, Efstathios K. Lang, Irene M. Lee, Ting- I. and Maerz, Winfried Nair, Sona B. Pascual, Gemma Peteiro, Jesus and Sakamoto, Choitsu Satomura, Atsushi Schiza, Sophia E. and Starkel, Peter Stojakovic, Tatjana Vesely, David L. Walters, Darren L. Yilmaz, Yusuf

Published
2012

32. Research update for articles published in EJCI in 2010

Author

UCL - SSS/IREC - Institut de recherche expérimentale et clinique, Adlbrecht, Christopher, Aigner, Elmar, Bellón, Juan M., Bouloukaki, Izolde, Bouzas-Mosquera, Alberto, Carrilho, Alexandre J.F., Chang, Kuo-Chu, Chattipakorn, Nipon, Chattipakorn, Siriporn C., Chen, Yi-Jen, Chung, Yuan-Chiang, Colah, Roshan, Datz, Christian, Frøkjær, Jens B., Fujimori, Shunji, Georgiadou, Panagiota, Grion, Cintia M., Hsu, Chih-Ping, Hülsmann, Martin, Hung, Ming-Jui, Hung, Ming-Yow, Iliodromitis, Efstathios K., Lang, Irene M., Lee, Ting-I., März, Winfried, Nair, Sona B., Pascual, Gemma, Peteiro, Jesús, Sakamoto, Choitsu, Satomura, Atsushi, Schiza, Sophia E., Starkel, Peter, Stojakovic, Tatjana, Vesely, David L., Walters, Darren L., Yilmaz, Yusuf, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, Adlbrecht, Christopher, Aigner, Elmar, Bellón, Juan M., Bouloukaki, Izolde, Bouzas-Mosquera, Alberto, Carrilho, Alexandre J.F., Chang, Kuo-Chu, Chattipakorn, Nipon, Chattipakorn, Siriporn C., Chen, Yi-Jen, Chung, Yuan-Chiang, Colah, Roshan, Datz, Christian, Frøkjær, Jens B., Fujimori, Shunji, Georgiadou, Panagiota, Grion, Cintia M., Hsu, Chih-Ping, Hülsmann, Martin, Hung, Ming-Jui, Hung, Ming-Yow, Iliodromitis, Efstathios K., Lang, Irene M., Lee, Ting-I., März, Winfried, Nair, Sona B., Pascual, Gemma, Peteiro, Jesús, Sakamoto, Choitsu, Satomura, Atsushi, Schiza, Sophia E., Starkel, Peter, Stojakovic, Tatjana, Vesely, David L., Walters, Darren L., and Yilmaz, Yusuf

Abstract

Research update for articles published in EJCI in 2010

Published
2012

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