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This study successfully used the modified fiber membrane to improve the filtration efficiency in the commercial rapid test strip. It can decrease the interference of the optical measurement or human eyes observation which was caused by the red blood cells. Our strategy was that added several modified fiber membranes above the commercial available blood separation area to enhance the filtration efficiency of the red blood cells. The fiber membrane had the specific pore size which can generate the physical barriers to remain the blood cells and used the chemical modification method to decrease the hemolysis and thrombus conditions in the rapid test strip.