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Several large or mid-scale collections of Drosophila enhancer traps have been recently created to allow for genetic swapping of GAL4 coding sequences to versatile transcription activators or suppressors such as LexA, QF, split-GAL4 (GAL4-AD and GAL4-DBD), GAL80 and QS. Yet a systematic analysis of the feasibility and reproducibility of these tools is lacking. Here we focused on InSITE GAL4 drivers that specifically label different subpopulations of olfactory neurons, particularly local interneurons (LNs), and genetically swapped the GAL4 domain for LexA, GAL80 or QF at the same locus. We found that the major utility-limiting factor for these genetic swaps is that many do not fully reproduce the original GAL4 expression patterns. Different donors exhibit distinct efficacies for reproducing original GAL4 expression patterns. The successfully swapped lines reported here will serve as valuable reagents and expand the genetic toolkits of Drosophila olfactory circuit research.

Social networking and cloud computing are being extensively used, and in this era, the frequency of sending information or images to each other is increasing. The prevention of private information leakage during communication over the Internet has become a concern in the past decades. Several data protection methods, such as cryptographic, watermarking, and steganography techniques, have been proposed to protect private data. In this paper, an embedding method is proposed based on an absolute moment block truncation coding (AMBTC)-compressed image. High and low mean tables are extracted from a compressed image and are divided into non-overlapping blocks. An adaptive variable N-bit bit plane truncation image embedding method is proposed to embed the secret data in each block. In this method, at the receiver end, the secret data are extracted, and the original AMBTC image could be recovered by recalling the stored peak and zero points. In addition, a chaotic encryption scheme is integrated into the proposed system to improve robustness against security vulnerability. The results show that the proposed method has superior performance and higher payload compared with the reference methods.

Drosophila olfactory local interneurons (LNs) in the antennal lobe are highly diverse and variable. How and when distinct types of LNs emerge, differentiate, and integrate into the olfactory circuit is unknown. Through systematic developmental analyses, we found that LNs are recruited to the adult olfactory circuit in three groups. Group 1 LNs are residual larval LNs. Group 2 are adult-specific LNs that emerge before cognate sensory and projection neurons establish synaptic specificity, and Group 3 LNs emerge after synaptic specificity is established. Group 1 larval LNs are selectively reintegrated into the adult circuit through pruning and re-extension of processes to distinct regions of the antennal lobe, while others die during metamorphosis. Precise temporal control of this pruning and cell death shapes the global organization of the adult antennal lobe. Our findings provide a road map to understand how LNs develop and contribute to constructing the olfactory circuit., Local interneurons (LNs) in the Drosophila olfactory system are highly diverse. Here, the authors labeled different LN types and described how different LN subtypes are integrated into the developing circuit.

Concomitant ductal carcinoma in situ (DCIS) and ipslateral gynecomastia (GM) are very rarely reported in male

With the current growth of multimedia applications, a substantial amount of visual data, including images and videos, is transmitted daily, making information forensics and security essential. Thus, protecting secret data from attackers is critical. In this work, we propose a AMBTC-based data-hiding method, in which secret data are concealed in individual image blocks that are compressed using absolute moment block truncation coding technique. By placing high or low mean values at the corner of each block, the secret data are then extracted reversibly and the original host image can be recovered at the decoding end. In addition, the quality of stego images is maintained, which protects the embedded visual data from the suspicion of attackers. From the experimental results, we show that the proposed method outperforms other state-of-the-art methods.

Objective There are conflicting data regarding the use of end-tidal carbon dioxide (PetCO2) measurement in preterm infants. The aim of this study was to evaluate the effects of different dead space to tidal volume ratios (VD/VT) on the correlation between PetCO2 and arterial carbon dioxide pressure (PaCO2) in ventilated preterm infants with respiratory distress syndrome (RDS). Methods We enrolled ventilated preterm infants (with assist control mode or synchronous intermittent mandatory mode) with RDS who were treated with surfactant in this prospective study. Simultaneous PetCO2 and PaCO2 data pairs were obtained from ventilated neonates monitored using mainstream capnography. Data obtained before and after surfactant treatment were also analyzed. Results One-hundred and one PetCO2 and PaCO2 pairs from 34 neonates were analyzed. There was a moderate correlation between PetCO2 and PaCO2 values (r = 0.603, P < 0.01). The correlation was higher in the post-surfactant treatment group (r = 0.786, P < 0.01) than the pre-surfactant treatment group (r = 0.235). The values of PaCO2 and PetCO2 obtained based on the treatment stage of surfactant therapy were 42.4 ± 8.6 mmHg and 32.6 ± 7.2 mmHg, respectively, in pre-surfactant treatment group, and 37.8 ± 10.3 mmHg and 33.7 ± 9.3 mmHg, respectively, in the post-surfactant treatment group. Furthermore, we found a significant decrease in VD/VT in the post-surfactant treatment group when compared to the pre-surfactant treatment group (P = 0.003). Conclusions VD/VT decreased significantly after surfactant therapy and the correlation between PetCO2 and PaCO2 was higher after surfactant therapy in preterm infants with RDS.

Because of stress and sleeping obstacle for 21th century people, the purpose of this study is to design a home decorative fabric which has the function of releasing from stress and enhancing sleeping quality. The optical fiber were sewn on the surface of optical fabric in form of three kinds of curvature patterns. Guiding the LED light into the optical fiber, the light will emit out of the optical fiber and stimulate our visual system in order to change the form of brain wave. Afterwards, the EEG instrument was used to evaluate the function of stress relaxation of such fabrics. The results were shown that human’s brain wave can be changed from relaxed and sober status to shallow-sleeping status after the stimulation of light emitting from the optical fiber fabrics. For the effect of causing shallow-sleeping status, female is more significant than male. Therefore, the light stimulating visual system by using the optical fiber fabrics can change the form of brain wave and achieve the effect of stress relaxation.

The thermal stability of CoSi2 layers on P-doped polycrystalline Si was investigated. It was observed that the additional Ge+ implant is performed prior to Co sputtering can suppress the CoSi2 agglomeration behavior. The samples with Ge+ implant at 50KeV implanted energy and more than or equal to the dose of 2E15 ions/cm2 show that the agglomeration of CoSi2 film is completely suppressed during high temperature RTA annealing. The grain size by the CoSi2 formation with the Ge+ implantation is much smaller than that without Ge+ implantation during post annealing. In addition, the cobalt atom in polysilicon layer with Ge+ implantation is shallower compared to without Ge+ implantation. The thermal stability of CoSi2 is significantly improved by adding the Ge+ implantation into polysilicon.

The original version of this Article contained errors in Figs. 4 and 6. In Fig. 4, panel a, text labels UAS-FLP and LexAop2>stop>myr::smGdP-HA were shifted upwards during typesetting of the figure, and in Fig. 6, panel h, the number 15 was incorrectly placed on the heat map scale. These have now been corrected in both the PDF and HTML versions of the Article.

For USJ engineering, cryogenic implantation is proposed for the reduction of dopant TED effect (i.e., Xj reduction) and the reduction of EOR defect formation. (i.e., less dopant deactivation)1 However, condensation defects are formed during the wafer warm up period due to the temperature of the cold wafer surface, and this impacts the device electrical performance. In this study, we use a heater lamp and post N 2 purge function to improve the lower wafer temperature issue after the cryogenic implantation process. In order to minimize the wafer to wafer temperature difference, we can adjust the wafer cassette elevator position and post N 2 purge time. Turn-on heater lamp 8min with elevator position at slot14 and post N 2 purge 40min, we can improve the wafer surface temperature to ∼23°C with wafer to wafer temperature range ∼0.6°C. The wafer surface temperature after the heater lamp function is close to room temperature implantation and it would be benefit to suppress the surface condensation defects formation.

Based on overcoming the sleeping obstacle for people, the purpose of this study is to design a photo-induced biofeedback fabric which is a kind of optical fiber fabric with the function of enhancing sleeping sense and to evaluate its effect. The fabrics with two layers including background layer and pattern layer were designed firstly. The pattern layers with 3 kinds of wavelengths of sine waves and the light controller with 3 kinds of flashing frequencies were then prepared. Guiding the light into the optical fiber, it will emit out of the optical fiber and stimulate our visual system to change the form of brain wave. Finally, EEG and sleeping scale were applied to evaluate the effect of enhancing sleeping sense. The results were shown that human’s brain wave can be changed from sober status to shallow-sleeping status and the effect of enhancing sleeping sense can be achieved for all pattern layers in frequencies of 0, 5 and 10 Hz. Furthermore, female is more significant than male and participants age from 30 to 49 are the most significant. Besides, the stronger the participant’s stress is, the more significant the sleeping sense is.

Background With the increasing interest with botulinum toxin – A (BTX-A) application in the lower urinary tract, we investigated the BTX-A effects on the canine prostate and also in men with bladder outlet obstruction (BOO) due to benign prostatic hyperplasia (BPH). Methods Transperineal injection into the prostate using transrectal ultrasound (TRUS) was performed throughout the study. Saline with or without 100 U of BTX-A was injected into mongrel dogs prostate. One or 3 months later, the prostate was harvested for morphologic and apoptotic study. In addition, eight BPH patients refractory to α-blockers were treated with ultrasound guided intraprostatic injection of 200 U of BTX-A. Results In the BTX-A treated dogs, atrophy and diffuse apoptosis was observed with H&E stain and TUNEL stain at 1 and 3 months. Clinically, the mean prostate volume, symptom score, and quality of life index were significantly reduced by 18.8%, 73.1%, and 61.5% respectively. Maximal flow rate significantly increased by 72.0%. Conclusion Intraprostatic BTX-A injection induces prostate apotosis in dogs and relieves BOO in humans. It is therefore a promising alternative treatment for refractory BOO due to BPH.