22 results on '"Hristova, Rositsa"'
Search Results
2. Effect of curcumin on γ–ray-induced cell response
- Author
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Kostova, Nora, primary, Staynova, Albena, additional, Popova-Hadjiiska, Ljubomira, additional, Georgieva, Dimka, additional, Ivanova, Ilonka, additional, Aneva, Nevena, additional, Atanasova, Margarita, additional, and Hristova, Rositsa, additional
- Published
- 2023
- Full Text
- View/download PDF
3. Investigating micronucleus assay applicability for prediction of normal tissue intrinsic radiosensitivity in gynecological cancer patients
- Author
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Encheva, Elitsa, Deleva, Sofia, Hristova, Rositsa, Hadjidekova, Valeria, and Hadjieva, Tatiana
- Published
- 2012
- Full Text
- View/download PDF
4. Effect of curcumin on proteasome activity in irradiated or non-irradiated lymphocytes
- Author
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Aneva, Nevena, primary, Kostova, Nora, additional, Ivanova, Ilonka, additional, Popova-Hadjiiska, Ljubomira, additional, and Hristova, Rositsa, additional
- Published
- 2021
- Full Text
- View/download PDF
5. Evaluating change in genomic frequency of translocations in 20 patients exposed to low-dose X-ray irradiation during routine vertebroplasty
- Author
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Ivanova, Ilonka, primary, Hristov, Hristo, additional, Tsonev, Hristo, additional, and Hristova, Rositsa, additional
- Published
- 2021
- Full Text
- View/download PDF
6. Cytogenetic follow-up study for over 4 years of three individuals accidentally exposed to 60Co in Bulgaria
- Author
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Staynova, Albena, primary, Georgieva, Dimka, additional, Popova, Ljubomira, additional, and Hristova, Rositsa, additional
- Published
- 2021
- Full Text
- View/download PDF
7. Does curcumin protect cellular DNA against γ-ray induced damage?
- Author
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Kostova, Nora, primary, Georgieva, Dimka, additional, Staynova, Albena, additional, Popova, Ljubomira, additional, Ivanova, Ilonka, additional, and Hristova, Rositsa, additional
- Published
- 2021
- Full Text
- View/download PDF
8. Radioprotective Effect of Curcumin on DNA Double Strand Breaks in Human Blood Lymphocytes after in vitro γ-Irradiation.
- Author
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Kostova, Nora, Staynova, Albena, Popova-Hadjiiska, Ljubomira, Georgieva, Dimka, Ivanova, Ilonka, and Hristova, Rositsa
- Subjects
DOUBLE-strand DNA breaks ,CURCUMIN ,CHROMOSOMAL translocation ,TURMERIC ,LYMPHOCYTES ,FLUORESCENCE in situ hybridization - Abstract
Curcumin is a component of natural spice Curcuma longa. It is known that this polyphenol has been shown to exhibit anti-inflammatory, anticancer, antiviral, antioxidant and antibacterial activities. The mechanism of curcumin effectiveness on both healthy and cancer tissues is still unclear. Aims: In vitro assessment of curcumin effect on both double-strand breaks and chromosomal translocations frequency, after γ-irradiation. Methods: Human peripheral blood samples were pre-treated with different concentrations of curcumin (0.5 μg/ml; 10 μg/ml; 20 μg/ml and 100 μg/ml). Thereafter they were exposed to 60Co γ-rays using various irradiation doses (0.05 Gy; 0.5 Gy; 1 Gy and 2 Gy). Both γ-H2AX/53BP1 foci assay and FISH analysis were used to evaluate DNA double-strand breaks and translocation frequencies. Results: Curcumin pre-treatment exhibited significant lower γ-H2AX/53BP1 foci appearance and reduced translocations frequency in irradiated compared to untreated lymphocytes. At 1 Gy irradiation and 10 μg/ml curcumin, the reduction of total translocations frequency was 42%. We found that at 2 Gy irradiation, the most protective concentration was 0.5 μg/ml curcumin. In this case, translocations declined almost twofold compared to curcumin nontreated cells. Conclusion: The present in vitro study demonstrates that curcumin reduces both γ-H2AX/53BP1 foci and translocations occurrence in peripheral blood lymphocytes, after γ-irradiation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
9. RENEB biodosimetry intercomparison analyzing translocations by FISH
- Author
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Barquinero, Joan Francesc, Beinke, Christina, Borras, Mireia, Buraczewska, Iwona, Darroudi, Firouz, Gregoire, Eric, Hristova, Rositsa, Kulka, Ulrike, Lindholm, Carita, Moreno, Mercedes, Moquet, Jayne, Oestreicher, Ursula, Prieto, M. Jesus, Pujol, Monica, Ricoul, Michelle, Sabatier, Laure, Sommer, Sylwester, Sun, Mingzhu, Wojcik, Andrzej, Leonardo Barrios, Universitat Autònoma de Barcelona (UAB), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Hospital General Universitario 'Gregorio Marañón' [Madrid], Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Public Health England [London], and Seventh Framework Programme, FP7 295513
- Subjects
[SDV]Life Sciences [q-bio] - Abstract
International audience; Purpose In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Materials and methods An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Results The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively. Conclusions The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH. © 2016 The Author(s). Published by Informa UK Limited, trading as Taylor and Francis Group.
- Published
- 2017
10. RENEB accident simulation exercise
- Author
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Brzozowska, Beata, Ainsbury, Elizabeth, Baert, Annelot, Beaton-Green, Lindsay, Barrios, Leonardo, Francesc Barquinero, Joan, Bassinet, Celine, Beinke, Christina, Benedek, Anett, Beukes, Philip, Bortolin, Emanuela, Buraczewska, Iwona, Burbidge, Christopher, De Amicis, Andrea, De Angelis, Cinzia, Della Monaca, Sara, Depuydt, Julie, De Sanctis, Stefania, Dobos, Katalin, Domene, Mercedes Moreno, Dominguez, Inmaculada, Facco, Eva, Fattibene, Paola, Frenzel, Monika, Gil, Octavia Monteiro, Gonon, Geraldine, Gregoire, Eric, Gruel, Gaetan, Hadjidekova, Valeria, Hatzi, Vasiliki I., Hristova, Rositsa, Jaworska, Alicja, Kis, Eniko, Kowalska, Maria, Kulka, Ulrike, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Meschini, Roberta, Moertl, Simone, Moquet, Jayne, Noditi, Mihaela, Oestreicher, Ursula, Orta Vazquez, Manuel Luis, Palma, Valentina, Pantelias, Gabriel, Montoro Pastor, Alegria, Patrono, Clarice, Piqueret-Stephan, Laure, Quattrini, Maria Cristina, Regalbuto, Elisa, Ricoul, Michelle, Roch-Lefevre, Sandrine, Roy, Laurence, Sabatier, Laure, Sarchiapone, Lucia, Sebastia, Natividad, Sommer, Sylwester, Sun, Mingzhu, Suto, Yumiko, Terzoudi, Georgia, Trompier, Francois, Vral, Anne, Wilkins, Ruth, Zafiropoulos, Demetre, Wieser, Albrecht, Woda, Clemens, Wojcik, Andrzej, Brzozowska, Beata, Ainsbury, Elizabeth, Baert, Annelot, Beaton-Green, Lindsay, Barrios, Leonardo, Francesc Barquinero, Joan, Bassinet, Celine, Beinke, Christina, Benedek, Anett, Beukes, Philip, Bortolin, Emanuela, Buraczewska, Iwona, Burbidge, Christopher, De Amicis, Andrea, De Angelis, Cinzia, Della Monaca, Sara, Depuydt, Julie, De Sanctis, Stefania, Dobos, Katalin, Domene, Mercedes Moreno, Dominguez, Inmaculada, Facco, Eva, Fattibene, Paola, Frenzel, Monika, Gil, Octavia Monteiro, Gonon, Geraldine, Gregoire, Eric, Gruel, Gaetan, Hadjidekova, Valeria, Hatzi, Vasiliki I., Hristova, Rositsa, Jaworska, Alicja, Kis, Eniko, Kowalska, Maria, Kulka, Ulrike, Lista, Florigio, Lumniczky, Katalin, Martinez-Lopez, Wilner, Meschini, Roberta, Moertl, Simone, Moquet, Jayne, Noditi, Mihaela, Oestreicher, Ursula, Orta Vazquez, Manuel Luis, Palma, Valentina, Pantelias, Gabriel, Montoro Pastor, Alegria, Patrono, Clarice, Piqueret-Stephan, Laure, Quattrini, Maria Cristina, Regalbuto, Elisa, Ricoul, Michelle, Roch-Lefevre, Sandrine, Roy, Laurence, Sabatier, Laure, Sarchiapone, Lucia, Sebastia, Natividad, Sommer, Sylwester, Sun, Mingzhu, Suto, Yumiko, Terzoudi, Georgia, Trompier, Francois, Vral, Anne, Wilkins, Ruth, Zafiropoulos, Demetre, Wieser, Albrecht, Woda, Clemens, and Wojcik, Andrzej
- Abstract
Purpose: The RENEB accident exercise was carried out in order to train the RENEB participants in coordinating and managing potentially large data sets that would be generated in case of a major radiological event. Materials and methods: Each participant was offered the possibility to activate the network by sending an alerting email about a simulated radiation emergency. The same participant had to collect, compile and report capacity, triage categorization and exposure scenario results obtained from all other participants. The exercise was performed over 27 weeks and involved the network consisting of 28 institutes: 21 RENEB members, four candidates and three non-RENEB partners. Results: The duration of a single exercise never exceeded 10 days, while the response from the assisting laboratories never came later than within half a day. During each week of the exercise, around 4500 samples were reported by all service laboratories (SL) to be examined and 54 scenarios were coherently estimated by all laboratories (the standard deviation from the mean of all SL answers for a given scenario category and a set of data was not larger than 3 patient codes). Conclusions: Each participant received training in both the role of a reference laboratory (activating the network) and of a service laboratory (responding to an activation request). The procedures in the case of radiological event were successfully established and tested.
- Published
- 2017
- Full Text
- View/download PDF
11. Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans - joint RENEB and EURADOS inter-laboratory comparisons
- Author
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Ainsbury, Elizabeth, Badie, Christophe, Barnard, Stephen, Manning, Grainne, Moquet, Jayne, Abend, Michael, Antunes, Ana Catarina, Barrios, Lleonard, Bassinet, Celine, Beinke, Christina, Bortolin, Emanuela, Bossin, Lily, Bricknell, Clare, Brzoska, Kamil, Buraczewska, Iwona, Huertas Castano, Carlos, Cemusova, Zina, Christiansson, Maria, Mateos Cordero, Santiago, Coster, Guillaume, Della Monac, Sara, Desangles, Francois, Discher, Michael, Dominguez, Inmaculada, Doucha-Senf, Sven, Eakins, Jon, Fattibene, Paola, Filippi, Silvia, Frenzel, Monika, Georgieva, Dimka, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Hadjiiska, Ljubomira, Hristova, Rositsa, Karakosta, Maria, Kis, Eniko, Kriehuber, Ralf, Lee, Jungil, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Macaeva, Ellina, Majewski, Matthaeus, Vanda Martins, S., McKeever, Stephen W. S., Meade, Aidan, Medipally, Dinesh, Meschini, Roberta, M'kacher, Radhia, Gil, Octavia Monteiro, Montero, Alegria, Moreno, Mercedes, Noditi, Mihaela, Oestreicher, Ursula, Oskamp, Dominik, Palitti, Fabrizio, Palma, Valentina, Pantelias, Gabriel, Pateux, Jerome, Patrono, Clarice, Pepe, Gaetano, Port, Matthias, Jesus Prieto, Maria, Quattrini, Maria Cristina, Quintens, Roel, Ricoul, Michelle, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Sholom, Sergey, Sommer, Sylwester, Staynova, Albena, Strunz, Sonja, Terzoudi, Georgia, Testa, Antonella, Trompier, Francois, Valente, Marco, Van Hoey, Olivier, Veronese, Ivan, Wojcik, Andrzej, Woda, Clemens, Ainsbury, Elizabeth, Badie, Christophe, Barnard, Stephen, Manning, Grainne, Moquet, Jayne, Abend, Michael, Antunes, Ana Catarina, Barrios, Lleonard, Bassinet, Celine, Beinke, Christina, Bortolin, Emanuela, Bossin, Lily, Bricknell, Clare, Brzoska, Kamil, Buraczewska, Iwona, Huertas Castano, Carlos, Cemusova, Zina, Christiansson, Maria, Mateos Cordero, Santiago, Coster, Guillaume, Della Monac, Sara, Desangles, Francois, Discher, Michael, Dominguez, Inmaculada, Doucha-Senf, Sven, Eakins, Jon, Fattibene, Paola, Filippi, Silvia, Frenzel, Monika, Georgieva, Dimka, Gregoire, Eric, Guogyte, Kamile, Hadjidekova, Valeria, Hadjiiska, Ljubomira, Hristova, Rositsa, Karakosta, Maria, Kis, Eniko, Kriehuber, Ralf, Lee, Jungil, Lloyd, David, Lumniczky, Katalin, Lyng, Fiona, Macaeva, Ellina, Majewski, Matthaeus, Vanda Martins, S., McKeever, Stephen W. S., Meade, Aidan, Medipally, Dinesh, Meschini, Roberta, M'kacher, Radhia, Gil, Octavia Monteiro, Montero, Alegria, Moreno, Mercedes, Noditi, Mihaela, Oestreicher, Ursula, Oskamp, Dominik, Palitti, Fabrizio, Palma, Valentina, Pantelias, Gabriel, Pateux, Jerome, Patrono, Clarice, Pepe, Gaetano, Port, Matthias, Jesus Prieto, Maria, Quattrini, Maria Cristina, Quintens, Roel, Ricoul, Michelle, Roy, Laurence, Sabatier, Laure, Sebastia, Natividad, Sholom, Sergey, Sommer, Sylwester, Staynova, Albena, Strunz, Sonja, Terzoudi, Georgia, Testa, Antonella, Trompier, Francois, Valente, Marco, Van Hoey, Olivier, Veronese, Ivan, Wojcik, Andrzej, and Woda, Clemens
- Abstract
Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation. Materials and methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiation induced thermoluminescent signals in glass screens taken from mobile phones. Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques. Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios.
- Published
- 2017
- Full Text
- View/download PDF
12. RENEB biodosimetry intercomparison analyzing translocations by FISH
- Author
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Francesc Barquinero, Joan, Beinke, Christina, Borras, Mireia, Buraczewska, Iwona, Darroudi, Firouz, Gregoire, Eric, Hristova, Rositsa, Kulka, Ulrike, Lindholm, Carita, Moreno, Mercedes, Moquet, Jayne, Oestreicher, Ursula, Jesus Prieto, M., Pujol, Monica, Ricoul, Michelle, Sabatier, Laure, Sommer, Sylwester, Sun, Mingzhu, Wojcik, Andrzej, Barrios, Leonardo, Francesc Barquinero, Joan, Beinke, Christina, Borras, Mireia, Buraczewska, Iwona, Darroudi, Firouz, Gregoire, Eric, Hristova, Rositsa, Kulka, Ulrike, Lindholm, Carita, Moreno, Mercedes, Moquet, Jayne, Oestreicher, Ursula, Jesus Prieto, M., Pujol, Monica, Ricoul, Michelle, Sabatier, Laure, Sommer, Sylwester, Sun, Mingzhu, Wojcik, Andrzej, and Barrios, Leonardo
- Abstract
Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Materials and methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively. Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.
- Published
- 2017
- Full Text
- View/download PDF
13. RENEB biodosimetry intercomparison analyzing translocations by FISH
- Author
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Barquinero, Joan Francesc, primary, Beinke, Christina, additional, Borràs, Mireia, additional, Buraczewska, Iwona, additional, Darroudi, Firouz, additional, Gregoire, Eric, additional, Hristova, Rositsa, additional, Kulka, Ulrike, additional, Lindholm, Carita, additional, Moreno, Mercedes, additional, Moquet, Jayne, additional, Oestreicher, Ursula, additional, Prieto, M Jesús, additional, Pujol, Mònica, additional, Ricoul, Michelle, additional, Sabatier, Laure, additional, Sommer, Sylwester, additional, Sun, Mingzhu, additional, Wojcik, Andrzej, additional, and Barrios, Leonardo, additional
- Published
- 2016
- Full Text
- View/download PDF
14. RENEB accident simulation exercise
- Author
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Brzozowska, Beata, primary, Ainsbury, Elizabeth, additional, Baert, Annelot, additional, Beaton-Green, Lindsay, additional, Barrios, Leonardo, additional, Barquinero, Joan Francesc, additional, Bassinet, Celine, additional, Beinke, Christina, additional, Benedek, Anett, additional, Beukes, Philip, additional, Bortolin, Emanuela, additional, Buraczewska, Iwona, additional, Burbidge, Christopher, additional, De Amicis, Andrea, additional, De Angelis, Cinzia, additional, Della Monaca, Sara, additional, Depuydt, Julie, additional, De Sanctis, Stefania, additional, Dobos, Katalin, additional, Domene, Mercedes Moreno, additional, Domínguez, Inmaculada, additional, Facco, Eva, additional, Fattibene, Paola, additional, Frenzel, Monika, additional, Monteiro Gil, Octávia, additional, Gonon, Géraldine, additional, Gregoire, Eric, additional, Gruel, Gaëtan, additional, Hadjidekova, Valeria, additional, Hatzi, Vasiliki I., additional, Hristova, Rositsa, additional, Jaworska, Alicja, additional, Kis, Enikő, additional, Kowalska, Maria, additional, Kulka, Ulrike, additional, Lista, Florigio, additional, Lumniczky, Katalin, additional, Martínez-López, Wilner, additional, Meschini, Roberta, additional, Moertl, Simone, additional, Moquet, Jayne, additional, Noditi, Mihaela, additional, Oestreicher, Ursula, additional, Orta Vázquez, Manuel Luis, additional, Palma, Valentina, additional, Pantelias, Gabriel, additional, Montoro Pastor, Alegria, additional, Patrono, Clarice, additional, Piqueret-Stephan, Laure, additional, Quattrini, Maria Cristina, additional, Regalbuto, Elisa, additional, Ricoul, Michelle, additional, Roch-Lefevre, Sandrine, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sarchiapone, Lucia, additional, Sebastià, Natividad, additional, Sommer, Sylwester, additional, Sun, Mingzhu, additional, Suto, Yumiko, additional, Terzoudi, Georgia, additional, Trompier, Francois, additional, Vral, Anne, additional, Wilkins, Ruth, additional, Zafiropoulos, Demetre, additional, Wieser, Albrecht, additional, Woda, Clemens, additional, and Wojcik, Andrzej, additional
- Published
- 2016
- Full Text
- View/download PDF
15. Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons
- Author
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Ainsbury, Elizabeth, primary, Badie, Christophe, additional, Barnard, Stephen, additional, Manning, Grainne, additional, Moquet, Jayne, additional, Abend, Michael, additional, Antunes, Ana Catarina, additional, Barrios, Lleonard, additional, Bassinet, Celine, additional, Beinke, Christina, additional, Bortolin, Emanuela, additional, Bossin, Lily, additional, Bricknell, Clare, additional, Brzoska, Kamil, additional, Buraczewska, Iwona, additional, Castaño, Carlos Huertas, additional, Čemusová, Zina, additional, Christiansson, Maria, additional, Cordero, Santiago Mateos, additional, Cosler, Guillaume, additional, Monaca, Sara Della, additional, Desangles, François, additional, Discher, Michael, additional, Dominguez, Inmaculada, additional, Doucha-Senf, Sven, additional, Eakins, Jon, additional, Fattibene, Paola, additional, Filippi, Silvia, additional, Frenzel, Monika, additional, Georgieva, Dimka, additional, Gregoire, Eric, additional, Guogyte, Kamile, additional, Hadjidekova, Valeria, additional, Hadjiiska, Ljubomira, additional, Hristova, Rositsa, additional, Karakosta, Maria, additional, Kis, Enikő, additional, Kriehuber, Ralf, additional, Lee, Jungil, additional, Lloyd, David, additional, Lumniczky, Katalin, additional, Lyng, Fiona, additional, Macaeva, Ellina, additional, Majewski, Matthaeus, additional, Vanda Martins, S., additional, McKeever, Stephen W.S., additional, Meade, Aidan, additional, Medipally, Dinesh, additional, Meschini, Roberta, additional, M’kacher, Radhia, additional, Gil, Octávia Monteiro, additional, Montero, Alegria, additional, Moreno, Mercedes, additional, Noditi, Mihaela, additional, Oestreicher, Ursula, additional, Oskamp, Dominik, additional, Palitti, Fabrizio, additional, Palma, Valentina, additional, Pantelias, Gabriel, additional, Pateux, Jerome, additional, Patrono, Clarice, additional, Pepe, Gaetano, additional, Port, Matthias, additional, Prieto, María Jesús, additional, Quattrini, Maria Cristina, additional, Quintens, Roel, additional, Ricoul, Michelle, additional, Roy, Laurence, additional, Sabatier, Laure, additional, Sebastià, Natividad, additional, Sholom, Sergey, additional, Sommer, Sylwester, additional, Staynova, Albena, additional, Strunz, Sonja, additional, Terzoudi, Georgia, additional, Testa, Antonella, additional, Trompier, Francois, additional, Valente, Marco, additional, Hoey, Olivier Van, additional, Veronese, Ivan, additional, Wojcik, Andrzej, additional, and Woda, Clemens, additional
- Published
- 2016
- Full Text
- View/download PDF
16. THE USE OF THE DICENTRIC ASSAY FOR BIOLOGICAL DOSIMETRY FOR RADIATION ACCIDENTS IN BULGARIA
- Author
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Hadjidekova, Valeria, primary, Hristova, Rositsa, additional, Ainsbury, Elizabeth A., additional, Atanasova, Petya, additional, Popova, Ljubomira, additional, and Staynova, Albena, additional
- Published
- 2010
- Full Text
- View/download PDF
17. Chromosome Abnormalities in Sperm From Infertile Men with Asthenoteratozoospermia1
- Author
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Hristova, Rositsa, primary, Ko, Evelyn, additional, Greene, Calvin, additional, Rademaker, Alfred, additional, Chernos, Judy, additional, and Martin, Renée, additional
- Published
- 2002
- Full Text
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18. Chromosome analysis of nuclear power plant workers using fluorescence in situ hybridization and Giemsa assay
- Author
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Hristova, Rositsa, Hadjidekova, Valeria, Grigorova, Mira, Nikolova, Teodora, Bulanova, Minka, Popova, Ljubomira, Staynova, Albena, and Benova, Donka
- Abstract
The aim of this study was to evaluate the genotoxic effects of ionizing radiation in vivo in exposed Bulgarian nuclear power plant workers by using classical cytogenetic and molecular cytogenetic analyses of peripheral lymphocytes. Chromosome analysis using fluorescence in situ hybrydization (FISH) and Giemsa techniques was undertaken on 63 workers and 45 administrative staff controls from the Bulgarian Nuclear Power Plant. Using the Giemsa method, the frequencies of cells studied with chromosome aberrations, dicentrics plus rings and chromosome fragments in the radiation workers were significantly higher compared with the control group (P = 0.044, P = 0.014, and P = 0.033, respectively). A significant association between frequencies of dicentrics plus rings and accumulated doses was registered (P < 0.01). In the present study, a FISH cocktail of whole chromosome paints for chromosomes 1, 4 and 11 was used. A significant association between frequency of translocations and accumulated doses was also observed (P < 0.001). Within the control group, a correlation was found between age and the spontaneous frequency of translocations. No correlation was found between smoking status and frequency of translocations. When compared with the control group, workers with accumulated doses up to 100 mSv showed no increase in genome translocation frequency, whereas workers with accumulated doses from 101 to 200 mSv showed a statistically significant doubling of genome translocation frequency (P = 0.009). Thus, in cases of chronic exposure and for purposes of retrospective dosimetry, the genome frequency of translocations is a more useful marker for evaluation of genotoxic effects than dicentric frequency.
- Published
- 2013
- Full Text
- View/download PDF
19. RENEB biodosimetry intercomparison analyzing translocations by FISH.
- Author
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Barquinero JF, Beinke C, Borràs M, Buraczewska I, Darroudi F, Gregoire E, Hristova R, Kulka U, Lindholm C, Moreno M, Moquet J, Oestreicher U, Prieto MJ, Pujol M, Ricoul M, Sabatier L, Sommer S, Sun M, Wojcik A, and Barrios L
- Subjects
- Biological Assay standards, Europe, Humans, In Situ Hybridization, Fluorescence standards, Lymphocytes radiation effects, Radiation Monitoring standards, Reproducibility of Results, Sensitivity and Specificity, Translocation, Genetic genetics, Biological Assay methods, In Situ Hybridization, Fluorescence methods, Quality Assurance, Health Care, Radiation Exposure analysis, Radiation Monitoring methods, Translocation, Genetic radiation effects
- Abstract
Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium., Materials and Methods: An initial telescoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates., Results: The coefficient of variation in the 1st IE (CV = 0.34) was higher than in the 2nd IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the 1st and 2nd IE, respectively., Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.
- Published
- 2017
- Full Text
- View/download PDF
20. RENEB accident simulation exercise.
- Author
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Brzozowska B, Ainsbury E, Baert A, Beaton-Green L, Barrios L, Barquinero JF, Bassinet C, Beinke C, Benedek A, Beukes P, Bortolin E, Buraczewska I, Burbidge C, De Amicis A, De Angelis C, Della Monaca S, Depuydt J, De Sanctis S, Dobos K, Domene MM, Domínguez I, Facco E, Fattibene P, Frenzel M, Monteiro Gil O, Gonon G, Gregoire E, Gruel G, Hadjidekova V, Hatzi VI, Hristova R, Jaworska A, Kis E, Kowalska M, Kulka U, Lista F, Lumniczky K, Martínez-López W, Meschini R, Moertl S, Moquet J, Noditi M, Oestreicher U, Orta Vázquez ML, Palma V, Pantelias G, Montoro Pastor A, Patrono C, Piqueret-Stephan L, Quattrini MC, Regalbuto E, Ricoul M, Roch-Lefevre S, Roy L, Sabatier L, Sarchiapone L, Sebastià N, Sommer S, Sun M, Suto Y, Terzoudi G, Trompier F, Vral A, Wilkins R, Zafiropoulos D, Wieser A, Woda C, and Wojcik A
- Subjects
- Europe, Disaster Planning organization & administration, Radiation Monitoring methods, Radioactive Hazard Release, Radiobiology education, Safety Management organization & administration, Triage organization & administration
- Abstract
Purpose: The RENEB accident exercise was carried out in order to train the RENEB participants in coordinating and managing potentially large data sets that would be generated in case of a major radiological event., Materials and Methods: Each participant was offered the possibility to activate the network by sending an alerting email about a simulated radiation emergency. The same participant had to collect, compile and report capacity, triage categorization and exposure scenario results obtained from all other participants. The exercise was performed over 27 weeks and involved the network consisting of 28 institutes: 21 RENEB members, four candidates and three non-RENEB partners., Results: The duration of a single exercise never exceeded 10 days, while the response from the assisting laboratories never came later than within half a day. During each week of the exercise, around 4500 samples were reported by all service laboratories (SL) to be examined and 54 scenarios were coherently estimated by all laboratories (the standard deviation from the mean of all SL answers for a given scenario category and a set of data was not larger than 3 patient codes)., Conclusions: Each participant received training in both the role of a reference laboratory (activating the network) and of a service laboratory (responding to an activation request). The procedures in the case of radiological event were successfully established and tested.
- Published
- 2017
- Full Text
- View/download PDF
21. Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans - joint RENEB and EURADOS inter-laboratory comparisons.
- Author
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Ainsbury E, Badie C, Barnard S, Manning G, Moquet J, Abend M, Antunes AC, Barrios L, Bassinet C, Beinke C, Bortolin E, Bossin L, Bricknell C, Brzoska K, Buraczewska I, Castaño CH, Čemusová Z, Christiansson M, Cordero SM, Cosler G, Monaca SD, Desangles F, Discher M, Dominguez I, Doucha-Senf S, Eakins J, Fattibene P, Filippi S, Frenzel M, Georgieva D, Gregoire E, Guogyte K, Hadjidekova V, Hadjiiska L, Hristova R, Karakosta M, Kis E, Kriehuber R, Lee J, Lloyd D, Lumniczky K, Lyng F, Macaeva E, Majewski M, Vanda Martins S, McKeever SW, Meade A, Medipally D, Meschini R, M'kacher R, Gil OM, Montero A, Moreno M, Noditi M, Oestreicher U, Oskamp D, Palitti F, Palma V, Pantelias G, Pateux J, Patrono C, Pepe G, Port M, Prieto MJ, Quattrini MC, Quintens R, Ricoul M, Roy L, Sabatier L, Sebastià N, Sholom S, Sommer S, Staynova A, Strunz S, Terzoudi G, Testa A, Trompier F, Valente M, Hoey OV, Veronese I, Wojcik A, and Woda C
- Subjects
- European Union, Reproducibility of Results, Retrospective Studies, Sensitivity and Specificity, Systems Integration, Biological Assay methods, Disaster Planning methods, Laboratories, Radiation Exposure analysis, Radiation Monitoring methods, Safety Management methods
- Abstract
Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation., Materials and Methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiation-induced thermoluminescent signals in glass screens taken from mobile phones., Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques., Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios.
- Published
- 2017
- Full Text
- View/download PDF
22. Investigating micronucleus assay applicability for prediction of normal tissue intrinsic radiosensitivity in gynecological cancer patients.
- Author
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Encheva E, Deleva S, Hristova R, Hadjidekova V, and Hadjieva T
- Abstract
Background: Pelvic organs morbidity after irradiation of cancer patients remains a major problem although new technologies have been developed and implemented. A relatively simple and suitable method for routine clinical practice is needed for preliminary assessment of normal tissue intrinsic radiosensitivity. The micronucleus test (MNT) determines the frequency of the radiation induced micronuclei (MN) in peripheral blood lymphocytes, which could serve as an indicator of intrinsic cell radiosensitivity., Aim: To investigate a possible use of the micronucleus test (MNT) for acute radiation morbidity prediction in gynecological cancer patients., Materials and Methods: Forty gynecological cancer patients received 50 Gy conventional external pelvic irradiation after radical surgery. A four-field "box" technique was applied with 2D planning. The control group included 10 healthy females. Acute normal tissue reactions were graded according to NCI CTCAE v.3.0. From all reaction scores, the highest score named "summarized clinical radiosensitivity" was selected for a statistical analysis. MNT was performed before and after in vitro irradiation with 1.5 Gy. The mean radiation induced frequency of micronuclei per 1000 binucleated cells (MN/1000) and lymphocytes containing micronuclei per 1000 binucleated cells (cells with MN/1000) were evaluated for both patients and controls. AN ARBITRARY CUT OFF VALUE WAS CREATED TO PICK UP A RADIOSENSITIVE INDIVIDUAL: the mean value of spontaneous frequency of cells with MN/1000 ± 2SD, found in the control group., Results: Both mean spontaneous frequency of cells with MN/1000 and MN/1000 were registered to be significantly higher in cancer patients compared to the control group (t = 2.46, p = 0.02 and t = 2.51, p = 0.02). No statistical difference was registered when comparing radiation induced MN frequencies between those groups. Eighty percent (32) of patients developed grade 2 summarized clinical radiosensitivity, with great variations in MNT parameters. Only three patients with grade 2 "summarized clinical radiosensitivity" had values of cells with MN/1000 above the chosen radiosensitivity threshold., Conclusion: The present study was not able to confirm in vitro MNT applicability for radiosensitivity prediction in pelvic irradiation.
- Published
- 2011
- Full Text
- View/download PDF
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