4 results on '"Hribar, Kaja"'
Search Results
2. Hyperglycaemia, pregnancy outcomes and maternal metabolic disease risk during pregnancy and lactation in a lean gestational diabetes mouse model
- Author
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Tol, Angela J C, Hribar, Kaja, Kruit, Janine, Bongiovanni, Laura, Vieira-Lara, Marcel A, Koster, Mirjam H, Kloosterhuis, Niels J, Havinga, Rick, Koehorst, Martijn, de Bruin, Alain, Bakker, Barbara M, Oosterveer, Maaike H, van der Beek, Eline M, Tol, Angela J C, Hribar, Kaja, Kruit, Janine, Bongiovanni, Laura, Vieira-Lara, Marcel A, Koster, Mirjam H, Kloosterhuis, Niels J, Havinga, Rick, Koehorst, Martijn, de Bruin, Alain, Bakker, Barbara M, Oosterveer, Maaike H, and van der Beek, Eline M
- Abstract
Abstract: Hyperglycaemia in pregnancy (HIP) is a pregnancy complication characterized by mild to moderate hyperglycaemia that negatively impacts short- and long-term health of mother and child. However, relationships between severity and timing of pregnancy hyperglycaemia and postpartum outcomes have not been systemically investigated. We investigated the impact of hyperglycaemia developing during pregnancy (gestational diabetes mellitus, GDM) or already present pre-mating (pre-gestational diabetes mellitus, PDM) on maternal health and pregnancy outcomes. GDM and PDM were induced in C57BL/6NTac mice by combined 60% high fat diet (HF) and low dose streptozotocin (STZ). Animals were screened for PDM prior to mating, and all underwent an oral glucose tolerance test on gestational day (GD)15. Tissues were collected at GD18 or at postnatal day (PN)15. Among HFSTZ-treated dams, 34% developed PDM and 66% developed GDM, characterized by impaired glucose-induced insulin release and inadequate suppression of endogenous glucose production. No increased adiposity or overt insulin resistance was observed. Furthermore, markers of non-alcoholic fatty liver disease (NAFLD) were significantly increased in PDM at GD18 and were positively correlated with basal glucose levels at GD18 in GDM dams. By PN15, NAFLD markers were also increased in GDM dams. Only PDM affected pregnancy outcomes such as litter size. Our findings indicate that GDM and PDM, resulting in disturbances of maternal glucose homeostasis, increase the risk of postpartum NAFLD development, related to the onset and severity of pregnancy hyperglycaemia. These findings signal a need for earlier monitoring of maternal glycaemia and more rigorous follow-up of maternal health after GDM and PDM pregnancy in humans. (Figure presented.). Key points: We studied the impact of high-fat diet/streptozotocin induced hyperglycaemia in pregnancy in mice and found that this impaired glucose tolerance and insulin release. Litter size a
- Published
- 2023
3. Hyperglycaemia, pregnancy outcomes and maternal metabolic disease risk during pregnancy and lactation in a lean gestational diabetes mouse model
- Author
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Pathobiologie, Dep Biomolecular Health Sciences, Cell Biology, Metabolism & Cancer - Cancer, Tol, Angela J C, Hribar, Kaja, Kruit, Janine, Bongiovanni, Laura, Vieira-Lara, Marcel A, Koster, Mirjam H, Kloosterhuis, Niels J, Havinga, Rick, Koehorst, Martijn, de Bruin, Alain, Bakker, Barbara M, Oosterveer, Maaike H, van der Beek, Eline M, Pathobiologie, Dep Biomolecular Health Sciences, Cell Biology, Metabolism & Cancer - Cancer, Tol, Angela J C, Hribar, Kaja, Kruit, Janine, Bongiovanni, Laura, Vieira-Lara, Marcel A, Koster, Mirjam H, Kloosterhuis, Niels J, Havinga, Rick, Koehorst, Martijn, de Bruin, Alain, Bakker, Barbara M, Oosterveer, Maaike H, and van der Beek, Eline M
- Published
- 2023
4. Skupne fenolne spojine in antioksidativna učinkovitost ekstrakta kimchija
- Author
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Hribar, Kaja and Pogačnik, Lea
- Subjects
fermentirana živila ,antioksidanti ,total phenolic compounds ,antioxidant activity ,fermented foods ,kimchi extracts ,Folin‒Ciocalteu ,Folin-Ciocalteu ,sok iz kimchija ,antioksidativna učinkovitost ,kimchi juice ,antioxidants ,analytic methods ,ekstrakt iz kimchija ,kimchi ,udc:663/664:547.56:543.2 ,fenolne spojine ,analizne metode ,DPPH - Abstract
Kimchi je tradicionalna fermentirana jed, ki izvira iz Koreje. Namen diplomskega dela je bilo pripraviti domači kimchi in ga primerjatis kupljenim. Tako iz kupljenega kot domačega kimchija smo želeli pripraviti vzorce soka in ekstrakte odcejenega kimchija ter v njih določiti skupne fenolne spojine in antioksidativno učinkovitost (AOU). V raziskavi smo uporabili pakirani korejski kimchi baechu znamke Chongga in kimchi, ki smo ga pripravili sami, in sicer s sedemdnevno in tridnevno fermentacijo ter sveži nefermentirani kimchi. V pripravljenih vzorcih soka in ekstrakta kimchija smo pred opravljanjem analiz določili vrednost pH z uporabo pH lističev. Vsebnost skupnih fenolnih spojin smo v vzorcih domačega in kupljenega kimchija določili spektrofotometrično z uporabo Folin‒Ciocalteujevega reagenta in rezultate analize izrazili kot ekvivalent klorogenske kisline. AOU soka in ekstrakta kimchija smo določili z metodo lovljenja radikala DPPH• in rezultate analize podali kot ekvivalent troloksa. Najvišjo AOU smo določili v vzorcu domačega kimchija, ki smo ga pustili fermentirati tri dni (0,22 mg/g v soku in 0,38 mg/g v ekstraktu), in v vzorcu kupljenega kimchija (0,24 mg/g v vzorcu soka in 0,44 mg/g v ekstraktu). Ta dva vzorca sta se tudi najbolj približala v literaturi opisani optimalni vrednosti pH 4,2. Povprečna vrednost skupnih fenolnih spojin v vzorcih soka domačega kimchija znaša 0,27 mg/g, v ekstraktu pa 0,33 mg/g. V soku kupljenega kimchija je vsebnost 0,53 mg/g, v ekstraktu pa 0,42 mg/g. Iz opravljene raziskave lahko sklepamo, da čas fermentacije in vrednost pH, ki jo živilo doseže, vplivata na vsebnost fenolnih spojin in AOU. Kimchi is a traditional Korean fermented vegetable containing several ingredients. The purpose of this thesis was to compare homemade nonfermented fresh kimchi as well as kimchi fermented for seven and three days with store-bought kimchi by determining antioxidant activity (AOU) and the total phenolic content in kimchi juice and extracts. In our research, we used beachu kimchi by brand Chongga as well as kimchi made at home from locally bought ingredients. We measured the pH value of our samples with the use of pH paper. Total phenolic content of extracts was determined spectrophotometrically according to Folin‒Ciocalteu method, the results of the analysis were expressed as an equivalent of the chlorogenic acid. AOU was evaluated using DPPH• radical scavenging method. AOU was expressed as trolox equivalent. The highest AOU was determined in homemade kimchi that was left to ferment for three days (0,22 mg/g in juice and 0,38 mg/g in extract) as well as in the store-bought kimchi (0,24 mg/g in juice and 0,44 mg/g in extract). The pH values of these samples were close to the optimum pH value reported elsewhere (pH 4,2). The average total phenolic compounds in homemade kimchi juice was 0,27 mg/g and in in extracts 0,33 mg/g. The total phenolic compounds in store-bought kimchi juice was 0,53 mg/g and in extract 0,42 mg/g. From our research it can be concluded that fermentation duration and the pH value of kimchi affects the total phenolic content and antioxidant activity.
- Published
- 2017
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