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3. Culture of human ovarian tissue in xeno-free conditions using laminin components of the human ovarian extracellular matrix

Author

Hao, J, Tuck, A R, Prakash, C R, Damdimopoulos, A, Sjödin, M O D, Lindberg, J, Niklasson, Boel, Pettersson, K, Hovatta, O, Damdimopoulou, P, Hao, J, Tuck, A R, Prakash, C R, Damdimopoulos, A, Sjödin, M O D, Lindberg, J, Niklasson, Boel, Pettersson, K, Hovatta, O, and Damdimopoulou, P

Abstract

PURPOSE: Our purpose was to identify human ovarian extracellular matrix (ECM) components that would support in vitro culture of human ovarian tissue and be compatible with possible future clinical applications. We characterized ovarian expression of laminins and selected three laminin tripeptides for culture experiments to be compared with Matrigel, an undefined and animal-based mixture of ECM components. METHODS: Expression of the 12 laminin genes was determined on transcript and protein levels using cortical tissue samples (n = 6), commercial ovary RNA (n = 1), follicular fluid granulosa cells (n = 20), and single-cell RNA-sequencing data. Laminin 221 (LN221), LN521, LN511, and their mixture were chosen for a 7-day culture experiment along with Matrigel using tissue from 17 patients. At the end of the culture, follicles were evaluated by scoring and counting from serial tissue sections, apoptosis measured using in situ TUNEL assay, proliferation by Ki67 staining, and endocrine function by quantifying steroids in culture media using UPLC-MS/MS. RESULTS: Approximately half of the cells in ovarian cortex expressed at least one laminin gene. The overall most expressed laminin α-chains were LAMA2 and LAMA5, β-chains LAMB1 and LAMB2, and γ-chain LAMC1. In culture experiments, LN221 enhanced follicular survival compared with Matrigel (p < 0.001), whereas tissue cultured on LN521 had higher proportion of secondary follicles (p < 0.001). LN511 and mixture of laminins did not support the cultures leading to lower follicle densities and higher apoptosis. All cultures produced steroids and contained proliferating cells. CONCLUSIONS: LN221 and LN521 show promise in providing xeno-free growth substrates for human ovarian tissue cultures, which may help in further development of folliculogenesis in vitro for clinical practices. The system could also be used for identification of adverse effects of chemicals in ovaries.

Published
2020
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20. Session 22. Assisted procreation

Author

Sunde, A., von During, V., Lippe, B., Siegel, J., Kahn, J., Molne, K., Roozenburg, B. J., Huisman, G. J., Zeilmaker, G.H., Alberda, A., Leerentveld, R. A., Liu, J., Van den Abbeel, E., Van Steirteghem, A., Rijnders, P. M., Jaarsma, E. Y., vanOs, H. C., Tombrock-Scheffer, M., Jansen, C. A. M., Smitz, J., Camus, M., Bollen, N., Tournaye, H., Staesen, C., Devroey, P., Weihs, D., Germond, M., Senn, A., Welti, H.J., De Grandi, P., Kururmäki, H., Ratsula, K., Lähteermäki, A., Hovatta, O., Bonanomi, S., and DeGrandi, P.

Published
2017

21. A missense mutation in SLC26A3 is associated with human male subfertility and impaired activation of CFTR

Author

Wedenoja, S. (Satu), Khamaysi, A. (Ahlam), Shimshilashvili, L. (Liana), Anbtawe-Jomaa, S. (Shireen), Elomaa, O. (Outi), Toppari, J. (Jorma), Höglund, P. (Pia), Aittomäki, K. (Kristiina), Holmberg, C. (Christer), Hovatta, O. (Outi), Tapanainen, J. S. (Juha S.), Ohana, E. (Ehud), Kere, J. (Juha), Wedenoja, S. (Satu), Khamaysi, A. (Ahlam), Shimshilashvili, L. (Liana), Anbtawe-Jomaa, S. (Shireen), Elomaa, O. (Outi), Toppari, J. (Jorma), Höglund, P. (Pia), Aittomäki, K. (Kristiina), Holmberg, C. (Christer), Hovatta, O. (Outi), Tapanainen, J. S. (Juha S.), Ohana, E. (Ehud), and Kere, J. (Juha)

Abstract

Chloride absorption and bicarbonate excretion through exchange by the solute carrier family 26 member 3 (SLC26A3) and cystic fibrosis transmembrane conductance regulator (CFTR) are crucial for many tissues including sperm and epithelia of the male reproductive tract. Homozygous SLC26A3 mutations cause congenital chloride diarrhea with male subfertility, while homozygous CFTR mutations cause cystic fibrosis with male infertility. Some homozygous or heterozygous CFTR mutations only manifest as male infertility. Accordingly, we studied the influence of SLC26A3 on idiopathic infertility by sequencing exons of SLC26A3 in 283 infertile and 211 control men. A heterozygous mutation c.2062 G > C (p.Asp688His) appeared in nine (3.2%) infertile men, and additionally, in two (0.9%) control men, whose samples revealed a sperm motility defect. The p.Asp688His mutation is localized in the CFTR-interacting STAS domain of SLC26A3 and enriched in Finland, showing a significant association with male infertility in comparison with 6,572 Finnish (P < 0.05) and over 120,000 global alleles (P < 0.0001) (ExAC database). Functional studies showed that while SLC26A3 is a strong activator of CFTR-dependent anion transport, SLC26A3-p.Asp688His mutant retains normal Cl⁻/HCO₃⁻ exchange activity but suppresses CFTR, despite unaffected domain binding and expression. These results suggest a novel mechanism for human male infertility─impaired anion transport by the coupled SLC26A3 and CFTR.

Published
2017

23. Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage

Author

Amps, K., Andrews, P.W., Anyfantis, G., Armstrong, L., Avery, S., Baharvand, H., Baker, J., Baker, D., Munoz, M.B., Beil, S., Benvenisty, N., Ben-Yosef, D., Biancotti, J.C., Bosman, A., Brena, R.M., Brison, D., Caisander, G., Camarasa, M.V., Chen, J.M., Chiao, E., Choi, Y.M., Choo, A.B.H., Collins, D., Colman, A., Crook, J.M., Daley, G.Q., Dalton, A., Sousa, P.A. de, Denning, C., Downie, J., Dvorak, P., Montgomery, K.D., Feki, A., Ford, A., Fox, V., Fraga, A.M., Frumkin, T., Ge, L., Gokhale, P.J., Golan-Lev, T., Gourabi, H., Gropp, M., Lu, G.X., Hampl, A., Harron, K., Healy, L., Herath, W., Holm, F., Hovatta, O., Hyllner, J., Inamdar, M.S., Irwanto, A.K., Ishii, T., Jaconi, M., Jin, Y., Kimber, S., Kiselev, S., Knowles, B.B., Kopper, O., Kukharenko, V., Kuliev, A., Lagarkova, M.A., Laird, P.W., Lako, M., Laslett, A.L., Lavon, N., Lee, D.R., Lee, J.E., Li, C.L., Lim, L.S., Ludwig, T.E., Ma, Y., Maltby, E., Mateizel, I., Mayshar, Y., Mileikovsky, M., Minger, S.L., Miyazaki, T., Moon, S.Y., Moore, H., Mummery, C., Nagy, A., Nakatsuji, N., Narwani, K., Oh, S.K.W., Oh, S.K., Olson, C., Otonkoski, T., Pan, F., Park, I.H., Pells, S., Pera, M.F., Pereira, L.V., Qi, O., Raj, G.S., Reubinoff, B., Robins, A., Robson, P., Rossant, J., Salekdeh, G.H., Schulz, T.C., Sermon, K., Mohamed, J.S., Shen, H., Sherrer, E., Sidhu, K., Sivarajah, S., Skottman, H., Spits, C., Stacey, G.N., Strehl, R., Strelchenko, N., Suemori, H., Sun, B.W., Suuronen, R., Takahashi, K., Tuuri, T., Venu, P., Verlinsky, Y., Ward-van Oostwaard, D., Weisenberger, D.J., Wu, Y., Yamanaka, S., Young, L., Zhou, Q., Int Stem Cell Initiative, Bosman, Alexis, Feki, Anis, and Jaconi, Marisa

Subjects
Inhibitor of Differentiation Protein 1, Cellular differentiation, Ethnic Groups/genetics, Chromosomes, Human, Pair 20, Inhibitor of Differentiation Protein 1/genetics/metabolism, Growth, ddc:616.07, Applied Microbiology and Biotechnology, 0302 clinical medicine, Induced Pluripotent Stem Cells/cytology, Ethnicity, Growth/genetics, Induced pluripotent stem cell, Genetics, CÉLULAS-TRONCO, 0303 health sciences, Gene Expression Regulation, Developmental, RNA-Binding Proteins, Cell Differentiation, Amplicon, Molecular Medicine, Stem cell, Biotechnology, Homeobox protein NANOG, Genotype, Cell Differentiation/genetics, Induced Pluripotent Stem Cells, bcl-X Protein, Biomedical Engineering, Bioengineering, Biology, Polymorphism, Single Nucleotide, Selection, Genetic/genetics, Cell Line, Clonal Evolution, 03 medical and health sciences, Bcl-X Protein/genetics/metabolism, Humans, RNA-Binding Proteins/genetics/metabolism, Selection, Genetic, Embryonic Stem Cells, 030304 developmental biology, Embryonic Stem Cells/cytology, Genetic Variation, DNA Methylation, Molecular biology, Embryonic stem cell, Chromosomes, Human, Pair 20/genetics, Clonal Evolution/genetics, Cell culture, Chromosome 20, 030217 neurology & neurosurgery
Abstract

The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells.

Published
2011

26. Points to Consider in the Development of Seed Stocks of Pluripotent Stem Cells for Clinical Applications: International Stem Cell Banking Initiative (ISCBI)

Author

Andrews, PW, primary, Baker, D, additional, Benvinisty, N, additional, Miranda, B, additional, Bruce, K, additional, Br…#x00FC;stle, O, additional, Choi, M, additional, Choi, Y-M, additional, Crook, JM, additional, de Sousa, PA, additional, Dvorak, P, additional, Freund, C, additional, Firpo, M, additional, Furue, MK, additional, Gokhale, P, additional, Ha, H-Y, additional, Han, E, additional, Haupt, S, additional, Healy, L, additional, Hei, DJ, additional, Hovatta, O, additional, Hunt, C, additional, Hwang, S-M, additional, Inamdar, MS, additional, Isasi, RM, additional, Jaconi, M, additional, Jekerle, V, additional, Kamthorn, P, additional, Kibbey, MC, additional, Knezevic, I, additional, Knowles, BB, additional, Koo, S-K, additional, Laabi, Y, additional, Leopoldo, L, additional, Liu, P, additional, Lomax, GP, additional, Loring, JF, additional, Ludwig, TE, additional, Montgomery, K, additional, Mummery, C, additional, Nagy, A, additional, Nakamura, Y, additional, Nakatsuji, N, additional, Oh, S, additional, Oh, S-K, additional, Otonkoski, T, additional, Pera, M, additional, Peschanski, M, additional, Pranke, P, additional, Rajala, KM, additional, Rao, M, additional, Ruttachuk, R, additional, Reubinoff, B, additional, Ricco, L, additional, Rooke, H, additional, Sipp, D, additional, Stacey, GN, additional, Suemori, H, additional, Takahashi, TA, additional, Takada, K, additional, Talib, S, additional, Tannenbaum, S, additional, Yuan, B-Z, additional, Zeng, F, additional, and Zhou, Q, additional

Published
2015
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27. Concomitant abuse of anabolic androgenic steroids and human chorionic gonadotrophin impairs spermatogenesis in power athletes

Author

Seppälä T, Karila T, and Hovatta O

Subjects
Adult, Male, endocrine system, medicine.medical_specialty, Weight Lifting, medicine.drug_class, Physical Therapy, Sports Therapy and Rehabilitation, Semen, Chorionic Gonadotropin, Male infertility, Semen quality, Anabolic Agents, Internal medicine, Medicine, Humans, Orthopedics and Sports Medicine, Spermatogenesis, Azoospermia, Doping in Sports, Dose-Response Relationship, Drug, Sperm Count, business.industry, medicine.disease, Sperm, Endocrinology, Concomitant, Androgens, Drug Therapy, Combination, Steroids, Gonadotropin, business
Abstract

Abuse of anabolic androgenic steroids (AASs) may be an aetiological factor in male infertility among recreational power athletes. They try to avoid AAS-induced deterioration in spermatogenesis by combining doses of human chorionic gonadotrophin (HCG) and/or antiestrogens with their AAS abuse. Eighteen healthy male power athletes using massive doses of AASs were recruited for the study. Semen samples were collected during AAS abuse and 1.5 and 6 months after cessation of the abuse. They were also asked about their reproductive activity six years after the study. At the end of the AAS cycle, the sperm count was 33 +/- 49 x 10 (6) /ml (mean +/- SD), and only one subject had azoospermia. At 1.5 months after cessation of the AAS cycles, the mean sperm concentration was 30 +/- 42 x 10 (6) /ml, and after six months 77 +/- 70 x 10 (6) /ml. There were significant differences between the sample drawn six months after cessation of AAS abuse and both samples drawn during and 1.5 months after the abuse (p

Published
2004

30. Recommendations for fertility preservation in patients with lymphoma, leukemia, and breast cancer

Author

Kim, SS, Donnez, J, Barri, P, Pellicer, A, Patrizio, P, Rosenwaks, Z, Nagy, P, Falcone, T, Andersen, C, Hovatta, O, Wallace, H, Meirow, D, Gook, D, Kim, SH, Tzeng, C-R, Suzuki, S, Ishizuka, B, Dolmans, M-M, Kim, SS, Donnez, J, Barri, P, Pellicer, A, Patrizio, P, Rosenwaks, Z, Nagy, P, Falcone, T, Andersen, C, Hovatta, O, Wallace, H, Meirow, D, Gook, D, Kim, SH, Tzeng, C-R, Suzuki, S, Ishizuka, B, and Dolmans, M-M

Abstract

Fertility issues should be addressed to all patients in reproductive age before cancer treatment. In men, cryopreservation of sperm should be offered to all cancer patients in reproductive age regardless of the risk of gonadal failure. In women, the recommendation of fertility preservation should be individualized based on multiple factors such as the urgency of treatment, the age of the patient, the marital status, the regimen and dosage of cancer treatment.

Published
2012

31. Genetic predictors of controlled ovarian hyperstimulation : where do we stand today?

Author

Altmäe, Signe, Hovatta, O., Stavreus-Evers, Anneli, Salumets, A., Altmäe, Signe, Hovatta, O., Stavreus-Evers, Anneli, and Salumets, A.

Abstract

BACKGROUND Nowadays, the use of IVF has improved the prospects of infertility treatment. The expected outcome of IVF depends greatly on the effectiveness of controlled ovarian hyperstimulation (COH), where exogenous gonadotrophins are used to induce folliculogenesis. The response to stimulation varies substantially among women and is difficult to predict. Several predictive markers of COH outcome have been proposed (e.g. maternal age and ovarian reserve), but the search for optimal predictors is ongoing. Pharmacogenetic studies demonstrate the effects of individual genetic variability on COH outcome and the potential for customizing therapy based on the patient's genome. METHODS MEDLINE, EMBASE, DARE, CINAHL and the Cochrane Library, and references from relevant articles were investigated up to February 2011 regarding any common genetic variation and COH/IVF outcome. RESULTS Several polymorphisms in genes involved in FSH signalling, estrogen biosynthesis, folliculogenesis, folate metabolism and other aspects influence the response to exogenous gonadotrophin administration, resulting in differences in COH and IVF outcomes. Nevertheless, the most studied polymorphism FSHR Asn680Ser is practically the only genetic marker, together with ESR1 PvuII T/C, that could be applied in clinical tests. CONCLUSIONS Although data are accumulating with evidence suggesting that the ovarian response to COH is mediated by various polymorphisms, the optimal biomarkers and the efficacy of the tests still remain to be evaluated.

Published
2011
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32. NANOG priming before full reprogramming may generate germ cell tumours

Author

Grad, I, Hibaoui, Y, Jaconi, M, Chicha, L, Bergström-Tengzelius, R, Sailani, M R, Pelte, M F, Dahoun, S, Mitsiadis, T A, Töhönen, V, Bouillaguet, S, Antonarakis, S E, Kere, J, Zucchelli, M, Hovatta, O, Feki, A, Grad, I, Hibaoui, Y, Jaconi, M, Chicha, L, Bergström-Tengzelius, R, Sailani, M R, Pelte, M F, Dahoun, S, Mitsiadis, T A, Töhönen, V, Bouillaguet, S, Antonarakis, S E, Kere, J, Zucchelli, M, Hovatta, O, and Feki, A

Abstract

Reprogramming somatic cells into a pluripotent state brings patient-tailored, ethical controversy-free cellular therapy closer to reality. However, stem cells and cancer cells share many common characteristics; therefore, it is crucial to be able to discriminate between them. We generated two induced pluripotent stem cell (iPSC) lines, with NANOG pre-transduction followed by OCT3/4, SOX2, and LIN28 overexpression. One of the cell lines, CHiPS W, showed normal pluripotent stem cell characteristics, while the other, CHiPS A, though expressing pluripotency markers, failed to differentiate and gave rise to germ cell-like tumours in vivo. Comparative genomic hybridisation analysis of the generated iPS lines revealed that they were genetically more stable than human embryonic stem cell counterparts. This analysis proved to be predictive for the differentiation potential of analysed cells. Moreover, the CHiPS A line expressed a lower ratio of p53/p21 when compared to CHiPS W. NANOG pre-induction followed by OCT3/4, SOX2, MYC, and KLF4 induction resulted in the same tumour-inducing phenotype. These results underline the importance of a re-examination of the role of NANOG during reprogramming. Moreover, this reprogramming method may provide insights into primordial cell tumour formation and cancer stem cell transformation.

Published
2011

33. Current achievements and future research directions in ovarian tissue culture, in vitro follicle development and transplantation : implications for fertility preservation

Author

UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie, UCL - (SLuc) Service de gynécologie et d'andrologie, UCL - (SLuc) Centre de thérapie tissulaire et cellulaire, Smitz, J., Dolmans, Marie-Madeleine, Donnez, Jacques, Fortune, J. E., Hovatta, O., Jewgenow, K., Picton, H. M., Plancha, C., Shea, L. D., Stouffer, R. L., Telfer, E. E., Woodruff, T. K., Zelinski, M. B., UCL - MD/GYPE - Département de gynécologie, d'obstétrique et de pédiatrie, UCL - (SLuc) Service de gynécologie et d'andrologie, UCL - (SLuc) Centre de thérapie tissulaire et cellulaire, Smitz, J., Dolmans, Marie-Madeleine, Donnez, Jacques, Fortune, J. E., Hovatta, O., Jewgenow, K., Picton, H. M., Plancha, C., Shea, L. D., Stouffer, R. L., Telfer, E. E., Woodruff, T. K., and Zelinski, M. B.

Abstract

Female cancer patients are offered 'banking' of gametes before starting fertility-threatening cancer therapy. Transplants of fresh and frozen ovarian tissue between healthy fertile and infertile women have demonstrated the utility of the tissue banked for restoration of endocrine and fertility function. Additional methods, like follicle culture and isolated follicle transplantation, are in development. Specialist reproductive medicine scientists and clinicians with complementary expertise in ovarian tissue culture and transplantation presented relevant published literature in their field of expertise and also unpublished promising data for discussion. As the major aims were to identify the current gaps prohibiting advancement, to share technical experience and to orient new research, contributors were allowed to provide their opinioned expert views on future research. Normal healthy children have been born in cancer survivors after orthotopic transplantation of their cryopreserved ovarian tissue. Longevity of the graft might be optimized by using new vitrification techniques and by promoting rapid revascularization of the graft. For the in vitro culture of follicles, a successive battery of culture methods including the use of defined media, growth factors and three-dimensional extracellular matrix support might overcome growth arrest of the follicles. Molecular methods and immunoassay can evaluate stage of maturation and guide adequate differentiation. Large animals, including non-human primates, are essential working models. Experiments on ovarian tissue from non-human primate models and from consenting fertile and infertile patients benefit from a multidisciplinary approach. The new discipline of oncofertility requires professionalization, multidisciplinarity and mobilization of funding for basic and translational research.

Published
2010

34. Comparison of defined culture systems for feeder cell free propagation of human embryonic stem cells

Author

Akopian, V, Andrews, PW, Beil, S, Benvenisty, N, Brehm, J, Christie, M, Ford, A, Fox, V, Gokhale, PJ, Healy, L, Holm, F, Hovatta, O, Knowles, BB, Ludwig, TE, McKay, RDG, Miyazaki, T, Nakatsuji, N, Oh, SKW, Pera, MF, Rossant, J, Stacey, GN, Suemori, H, Akopian, V, Andrews, PW, Beil, S, Benvenisty, N, Brehm, J, Christie, M, Ford, A, Fox, V, Gokhale, PJ, Healy, L, Holm, F, Hovatta, O, Knowles, BB, Ludwig, TE, McKay, RDG, Miyazaki, T, Nakatsuji, N, Oh, SKW, Pera, MF, Rossant, J, Stacey, GN, and Suemori, H

Abstract

There are many reports of defined culture systems for the propagation of human embryonic stem cells in the absence of feeder cell support, but no previous study has undertaken a multi-laboratory comparison of these diverse methodologies. In this study, five separate laboratories, each with experience in human embryonic stem cell culture, used a panel of ten embryonic stem cell lines (including WA09 as an index cell line common to all laboratories) to assess eight cell culture methods, with propagation in the presence of Knockout Serum Replacer, FGF-2, and mouse embryonic fibroblast feeder cell layers serving as a positive control. The cultures were assessed for up to ten passages for attachment, death, and differentiated morphology by phase contrast microscopy, for growth by serial cell counts, and for maintenance of stem cell surface marker expression by flow cytometry. Of the eight culture systems, only the control and those based on two commercial media, mTeSR1 and STEMPRO, supported maintenance of most cell lines for ten passages. Cultures grown in the remaining media failed before this point due to lack of attachment, cell death, or overt cell differentiation. Possible explanations for relative success of the commercial formulations in this study, and the lack of success with other formulations from academic groups compared to previously published results, include: the complex combination of growth factors present in the commercial preparations; improved development, manufacture, and quality control in the commercial products; differences in epigenetic adaptation to culture in vitro between different ES cell lines grown in different laboratories.

Published
2010

35. Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells

Author

Unger, C, Gao, S, Cohen, M, Jaconi, M, Bergstrom, R, Holm, F, Galan, A, Sanchez, E, Irion, O, Dubuisson, J B, Giry-Laterriere, M, Salmon, P, Simon, C, Hovatta, O, Feki, A, Unger, C, Gao, S, Cohen, M, Jaconi, M, Bergstrom, R, Holm, F, Galan, A, Sanchez, E, Irion, O, Dubuisson, J B, Giry-Laterriere, M, Salmon, P, Simon, C, Hovatta, O, and Feki, A

Abstract

BACKGROUND: Feeder cells are frequently used for the early-stage of derivation and culture of human embryonic stem cell (hESC) lines. METHODS: We established a conditionally immortalized human foreskin fibroblast line that secreted basic fibroblast growth factor (bFGF). These cells were used as feeder cells for hESC culture and induced pluripotent stem (iPS) cell derivation and expansion. This conditional immortalization was performed using lentiviral vector (LV) mediated transduction of Bmi-1 and human telomerase reverse transcriptase genes and the resulting cell line was further modified by LV-mediated transduction of a secreted form of bFGF gene product. Three different laboratories have tested whether this feeder cell line could support the maintenance of four different hESC lines. RESULTS: Immortalized fibroblasts secreting stable amounts of bFGF supported the growth of all hESC lines, which remained pluripotent and had a normal karyotype for at least 10 passages. Even at high passage (p56), these modified cells, when used as feeders, could support iPS derivation and propagation. Derived iPS cells expressed pluripotency markers, had hESC morphology and produced tissue components of the three germ layers when differentiated in vitro. CONCLUSION: These modified fibroblasts are useful as a genetically-defined feeder cell line for reproducible and cost-effective culture of both hESC and iPS cells.

Published
2009

38. Markers of Pluripotency and Differentiation in Human Neural Precursor Cells Derived from Embryonic Stem Cells and CNS Tissue

Author

Sundberg, M., primary, Andersson, P.-H., additional, Åkesson, E., additional, Odeberg, J., additional, Holmberg, L., additional, Inzunza, J., additional, Falci, S., additional, Öhman, J., additional, Suuronen, R., additional, Skottman, H., additional, Lehtimäki, K., additional, Hovatta, O., additional, Narkilahti, S., additional, and Sundström, E., additional

Published
2011
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40. POSTER VIEWING SESSION - EMBRYOLOGY

Author

Fourati Ben Mustapha, S., primary, Khrouf, M., additional, Kacem Ben Rejeb, K., additional, Elloumi Chaabene, H., additional, Merdassi, G., additional, Wahbi, D., additional, Ben Meftah, M., additional, Zhioua, F., additional, Zhioua, A., additional, Azzarello, A., additional, Host, T., additional, Mikkelsen, A. L., additional, Theofanakis, C. P., additional, Dinopoulou, V., additional, Mavrogianni, D., additional, Partsinevelos, G. A., additional, Drakakis, P., additional, Stefanidis, K., additional, Bletsa, A., additional, Loutradis, D., additional, Rienzi, L., additional, Cobo, A., additional, Paffoni, A., additional, Scarduelli, C., additional, Capalbo, A., additional, Garrido, N., additional, Remohi, J., additional, Ragni, G., additional, Ubaldi, F. M., additional, Herrer, R., additional, Quera, M., additional, GIL, E., additional, Serna, J., additional, Grondahl, M. L., additional, Bogstad, J., additional, Agerholm, I. E., additional, Lemmen, J. G., additional, Bentin-Ley, U., additional, Lundstrom, P., additional, Kesmodel, U. S., additional, Raaschou-Jensen, M., additional, Ladelund, S., additional, Guzman, L., additional, Ortega, C., additional, Albuz, F. K., additional, Gilchrist, R. B., additional, Devroey, P., additional, Smitz, J., additional, De Vos, M., additional, Bielanska, M., additional, Leveille, M. C., additional, Borghi, E., additional, Magli, M. C., additional, Figueroa, M. J., additional, Mascaretti, G., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Szlit, E., additional, Leocata Nieto, F., additional, Maggiotto, G., additional, Arenas, G., additional, Tarducci Bonfiglio, N., additional, Ahumada, A., additional, Asch, R., additional, Sciorio, R., additional, Dayoub, N., additional, Thong, J., additional, Pickering, S., additional, Ten, J., additional, Carracedo, M. A., additional, Guerrero, J., additional, Rodriguez-Arnedo, A., additional, Llacer, J., additional, Bernabeu, R., additional, Tatone, C., additional, Heizenrieder, T., additional, Di Emidio, G., additional, Treffon, P., additional, Seidel, T., additional, Eichenlaub-Ritter, U., additional, Cortezzi, S. S., additional, Cabral, E. C., additional, Ferreira, C. R., additional, Trevisan, M. G., additional, Figueira, R. C. S., additional, Braga, D. P. A. F., additional, Eberlin, M. N., additional, Iaconelli Jr., A., additional, Borges Jr., E., additional, Zabala, A., additional, Pessino, T., additional, Blanco, L., additional, Rey Valzacchi, G., additional, Leocata, F., additional, Vanden Meerschaut, F., additional, Heindryckx, B., additional, Qian, C., additional, Deforce, D., additional, Leybaert, L., additional, De Sutter, P., additional, De las Heras, M., additional, De Pablo, J. L., additional, Navarro, B., additional, Agirregoikoa, J. A., additional, Barrenetxea, G., additional, Cruz, M., additional, Perez-Cano, I., additional, Gadea, B., additional, Herrero, J., additional, Martinez, M., additional, Roldan, M., additional, Munoz, M., additional, Pellicer, A., additional, Meseguer, M., additional, Galindo, N., additional, Scarselli, F., additional, Alviggi, E., additional, Colasante, A., additional, Minasi, M. G., additional, Rubino, P., additional, Lobascio, M., additional, Ferrero, S., additional, Litwicka, K., additional, Varricchio, M. T., additional, Giannini, P., additional, Piscitelli, P., additional, Franco, G., additional, Zavaglia, D., additional, Nagy, Z. P., additional, Greco, E., additional, Urner, F., additional, Wirthner, D., additional, Murisier, F., additional, Mock, P., additional, Germond, M., additional, Amorocho Llanos, B., additional, Calderon, G., additional, Lopez, D., additional, Fernandez, L., additional, Nicolas, M., additional, Landeras, J., additional, Finn-Sell, S. L., additional, Leandri, R., additional, Fleming, T. P., additional, Macklon, N. S., additional, Cheong, Y. C., additional, Eckert, J. J., additional, Lee, J. H., additional, Jung, Y. J., additional, Hwang, H. K., additional, Kang, A., additional, An, S. J., additional, Jung, J. Y., additional, Kwon, H. C., additional, Lee, S. J., additional, Palini, S., additional, Zolla, L., additional, De Stefani, S., additional, Scala, V., additional, D'Alessandro, A., additional, Polli, V., additional, Rocchi, P., additional, Tiezzi, A., additional, Pelosi, E., additional, Dusi, L., additional, Bulletti, C., additional, Fadini, R., additional, Lain, M., additional, Mignini Renzini, M., additional, Brambillasca, F., additional, Coticchio, G., additional, Merola, M., additional, Guglielmo, M. C., additional, Dal Canto, M., additional, Figueira, R., additional, Setti, A. S., additional, Worrilow, K. C., additional, Uzochukwu, C. D., additional, Eid, S., additional, Le Gac, S., additional, Esteves, T. C., additional, van Rossem, F., additional, van den Berg, A., additional, Boiani, M., additional, Kasapi, E., additional, Panagiotidis, Y., additional, Goudakou, M., additional, Papatheodorou, A., additional, Pasadaki, T., additional, Prapas, N., additional, Prapas, Y., additional, Vanderzwalmen, P., additional, Norasing, S., additional, Atchajaroensatit, P., additional, Tawiwong, W., additional, Thepmanee, O., additional, Saenlao, S., additional, Aojanepong, J., additional, Hunsajarupan, P., additional, Sajjachareonpong, K., additional, Punyatanasakchai, P., additional, Maneepalviratn, S., additional, Jetsawangsri, U., additional, Tejera, A., additional, Rubio, I., additional, Romero, J. L., additional, Nordhoff, V., additional, Schlatt, S., additional, Schuring, A. N., additional, Kiesel, L., additional, Kliesch, S., additional, Azambuja, R., additional, Okada, L., additional, Lazzari, V., additional, Dorfman, L., additional, Michelon, J., additional, Badalotti, M., additional, Badalotti, F., additional, Petracco, A., additional, Schwarzer, C., additional, Versieren, K., additional, De Croo, I., additional, Lierman, S., additional, De Vos, W., additional, Van den Abbeel, E., additional, Gerris, J., additional, Milacic, I., additional, Borogovac, D., additional, Veljkovic, M., additional, Arsic, B., additional, Jovic Bojovic, D., additional, Lekic, D., additional, Pavlovic, D., additional, Garalejic, E., additional, Albertini, D. F., additional, De Ponti, E., additional, Sanges, F., additional, Talevi, R., additional, Papini, L., additional, Mollo, V., additional, Rienzi, L. F., additional, Gualtieri, R., additional, Orteg, C., additional, Choi, J., additional, Lee, H., additional, Ku, S., additional, Kim, S., additional, Choi, Y., additional, Kim, J., additional, Moon, S., additional, Demilly, E., additional, Assou, S., additional, Moussaddykine, S., additional, Dechaud, H., additional, Hamamah, S., additional, Takisawa, T., additional, Doshida, M., additional, Hattori, H., additional, Nakamura, Y., additional, Kyoya, T., additional, Shibuya, Y., additional, Nakajo, Y., additional, Tasaka, A., additional, Toya, M., additional, Kyono, K., additional, Novo, S., additional, Penon, O., additional, Gomez, R., additional, Barrios, L., additional, Duch, M., additional, Santalo, J., additional, Esteve, J., additional, Nogues, C., additional, Plaza, J. A., additional, Perez-Garcia, L., additional, Ibanez, E., additional, Chavez, S., additional, Loewke, K., additional, Behr, B., additional, Reijo Pera, R., additional, Huang, S., additional, Wang, H., additional, Soong, Y., additional, Chang, C., additional, Okimura, T., additional, Kuwayama, M., additional, Mori, C., additional, Morita, M., additional, Uchiyama, K., additional, Aono, F., additional, Kato, K., additional, Takehara, Y., additional, Kato, O., additional, Minasi, M., additional, Casciani, V., additional, Arizzi, L., additional, Mencacci, C., additional, Piscitelli, C., additional, Cucinelli, F., additional, Tocci, A., additional, Wydooghe, E., additional, Vandaele, L., additional, Dewulf, J., additional, Van Soom, A., additional, Moon, J. H., additional, Son, W. Y., additional, Mahfoudh, A., additional, Henderson, S., additional, Jin, S. G., additional, Shalom-Paz, E., additional, Dahan, M., additional, Holzer, H., additional, Mahmoud, K., additional, Triki-Hmam, C., additional, Terras, K., additional, Hfaiedh, T., additional, Ben Aribia, M. H., additional, Otsubo, H., additional, Egashira, A., additional, Tanaka, K., additional, Matsuguma, T., additional, Murakami, M., additional, Murakami, K., additional, Otsuka, M., additional, Yoshioka, N., additional, Araki, Y., additional, Kuramoto, T., additional, Smit, J. G., additional, Sterrenburg, M. D., additional, Eijkemans, M. J. C., additional, Al-Inany, H. G., additional, Youssef, M. A. F. M., additional, Broekmans, F. J. M., additional, Willoughby, K., additional, DiPaolo, L., additional, Deys, L., additional, Lagunov, A., additional, Amin, S., additional, Faghih, M., additional, Hughes, E., additional, Karnis, M., additional, Ashkar, F., additional, King, W. A., additional, Neal, M. S., additional, Antonova, I., additional, Veleva, L., additional, Petkova, L., additional, Shterev, A., additional, Nogales, C., additional, Martinez, E., additional, Ariza, M., additional, Cernuda, D., additional, Gaytan, M., additional, Linan, A., additional, Guillen, A., additional, Bronet, F., additional, Cottin, V., additional, Fabian, D., additional, Allemann, F., additional, Koller, A., additional, Spira, J. C., additional, Agudo, D., additional, Martinez-Burgos, M., additional, Arnanz, A., additional, Basile, N., additional, Rodriguez, A., additional, Cho, Y. S., additional, Filioli Uranio, M., additional, Ambruosi, B., additional, Paternoster, M. S., additional, Totaro, P., additional, Sardanelli, A. M., additional, Dell'Aquila, M. E., additional, Zollner, U., additional, Hofmann, T., additional, Zollner, K. P., additional, Kovacic, B., additional, Roglic, P., additional, Vlaisavljevic, V., additional, Sole, M., additional, Boada, M., additional, Coroleu, B., additional, Veiga, A., additional, Martiny, G., additional, Molinari, M., additional, Revelli, A., additional, Chimote, N. M., additional, Chimote, M., additional, Mehta, B., additional, Chimote, N. N., additional, Sheikh, N., additional, Nath, N., additional, Mukherjee, A., additional, Rakic, K., additional, Reljic, M., additional, Ingerslev, H. J., additional, Kirkegaard, K., additional, Hindkjaer, J., additional, Agerholm, I., additional, Kitasaka, H., additional, Fukunaga, N., additional, Nagai, R., additional, Yoshimura, T., additional, Tamura, F., additional, Kitamura, K., additional, Hasegawa, N., additional, Nakayama, K., additional, Katou, M., additional, Itoi, F., additional, Asano, E., additional, Deguchi, N., additional, Ooyama, K., additional, Hashiba, Y., additional, Asada, Y., additional, Michaeli, M., additional, Rotfarb, N., additional, Karchovsky, E., additional, Ruzov, O., additional, Atamny, R., additional, Slush, K., additional, Fainaru, O., additional, Ellenbogen, A., additional, Chekuri, S., additional, Chaisrisawatsuk, T., additional, Chen, P., additional, Pangestu, M., additional, Jansen, S., additional, Catt, S., additional, Molinari, E., additional, Racca, C., additional, Ryu, C., additional, Kang, S., additional, Lee, J., additional, Chung, D., additional, Roh, S., additional, Chi, H., additional, Yokota, Y., additional, Yokota, M., additional, Yokota, H., additional, Sato, S., additional, Nakagawa, M., additional, Komatsubara, M., additional, Makita, M., additional, Oyama, K., additional, Naruse, K., additional, Kilani, S., additional, Chapman, M. G., additional, Kwik, M., additional, Chapman, M., additional, Guven, S., additional, Odaci, E., additional, Yildirim, O., additional, Kart, C., additional, Unsal, M. A., additional, Yulug, E., additional, Isachenko, E., additional, Maettner, R., additional, Strehler, E., additional, Isachenko, V., additional, Hancke, K., additional, Kreienberg, R., additional, Sterzik, K., additional, Zheng, X. Y., additional, Wang, L. N., additional, Liu, P., additional, Qiao, J., additional, Inoue, F., additional, Dashtizad, M., additional, Wahid, H., additional, Rosnina, Y., additional, Daliri, M., additional, Hajarian, H., additional, Akbarpour, M., additional, Abbas Mazni, O., additional, Knez, K., additional, Tomaevic, T., additional, Vrtacnik Bokal, E., additional, Zorn, B., additional, Virant Klun, I., additional, Koster, M., additional, Liebenthron, J., additional, Nicolov, A., additional, van der Ven, K., additional, van der Ven, H., additional, Montag, M., additional, Fayazi, M., additional, Salehnia, M., additional, Beigi Boroujeni, M., additional, Khansarinejad, B., additional, Deignan, K., additional, Emerson, G., additional, Mocanu, E., additional, Wang, J. J., additional, Andonov, M., additional, Linara, E., additional, Ahuja, K. K., additional, Nachef, S., additional, Pasqualotto, F. F., additional, Pasqualotto, E., additional, Chang, C. C., additional, Bernal, D. P., additional, Elliott, T. A., additional, Shapiro, D. B., additional, Toledo, A. A., additional, Economou, K., additional, Davies, S., additional, Argyrou, M., additional, Doriza, S., additional, Sisi, P., additional, Moschopoulou, M., additional, Karagianni, A., additional, Mendorou, C., additional, Polidoropoulos, N., additional, Papanicopoulos, C., additional, Stefanis, P., additional, Karamalegos, C., additional, Cazlaris, H., additional, Koutsilieris, M., additional, Mastrominas, M., additional, Gotts, S., additional, Doshi, A., additional, Harper, J., additional, Serhal, P., additional, Borini, A., additional, Guzeloglu-Kayisli, O., additional, Bianchi, V., additional, Seli, E., additional, Lappi, M., additional, Bonu, M. A., additional, Mizuta, S., additional, Hashimoto, H., additional, Kuroda, Y., additional, Matsumoto, Y., additional, Mizusawa, Y., additional, Ogata, S., additional, Yamada, S., additional, Kokeguchi, S., additional, Noda, Y., additional, Shiotani, M., additional, Stojkovic, M., additional, Ilic, M., additional, Markovic, N., additional, Stojkovic, P., additional, Feng, G., additional, Zhang, B., additional, Zhou, H., additional, Zhou, L., additional, Gan, X., additional, Qin, X., additional, Shu, J., additional, Wu, F., additional, Molina Botella, I., additional, Lazaro Ibanez, E., additional, Debon Aucejo, A., additional, Pertusa, J., additional, Fernandez Colom, P. J., additional, Li, C., additional, Zhang, Y., additional, Cui, Y., additional, Zhao, H., additional, Liu, J., additional, Oliveira, J. B. A., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Silva, L. F. I., additional, Ricci, J., additional, Cavagna, M., additional, Pontes, A., additional, Vagnini, L. D., additional, Baruffi, R. L. R., additional, Franco Jr., J. G., additional, Felipe, V., additional, Vilela, M., additional, Tiveron, M., additional, Lombardi, C., additional, Viglierchio, M. I., additional, Marconi, G., additional, Rawe, V., additional, Wale, P. L., additional, Gardner, D. K., additional, Nakagawa, K., additional, Sugiyama, R., additional, Nishi, Y., additional, Kuribayashi, Y., additional, Jyuen, H., additional, Yamashiro, E., additional, Shirai, A., additional, Inoue, M., additional, Hovatta, O., additional, Tohonen, V., additional, Inzunza, J., additional, Parmegiani, L., additional, Cognigni, G. E., additional, Bernardi, S., additional, Ciampaglia, W., additional, Infante, F. E., additional, Tabarelli de Fatis, C., additional, Pocognoli, P., additional, Arnone, A., additional, Maccarini, A. M., additional, Troilo, E., additional, Filicori, M., additional, Radwan, P., additional, Polac, I., additional, Borowiecka, M., additional, Bijak, M., additional, and Radwan, M., additional

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2011
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41. POSTER VIEWING SESSION - ENDOMETRIOSIS, ENDOMETRIUM, IMPLANTATION AND FALLOPIAN TUBE

Author

Palial, K. K., primary, Drury, J., additional, Heathcote, L., additional, Valentijin, A., additional, Farquharson, R. G., additional, Gazvani, R., additional, Rudland, P. S., additional, Hapangama, D. K., additional, Celik, N., additional, Celik, O., additional, Aktan, E., additional, Ozerol, E., additional, Celik, E., additional, Bozkurt, K., additional, Paran, H., additional, Hascalik, S., additional, Ozerol, I., additional, Arase, T., additional, Maruyama, T., additional, Uchida, H., additional, Miyazaki, K., additional, Oda, H., additional, Uchida-Nishikawa, S., additional, Kagami, M., additional, Yamazaki, A., additional, Tamaki, K., additional, Yoshimura, Y., additional, De Vos, M., additional, Ortega, C., additional, Smitz, J., additional, Van Vaerenbergh, I., additional, Bourgain, C., additional, Devroey, P., additional, Luciano, D., additional, Exacoustos, C., additional, Zupi, E., additional, Luciano, A. A., additional, Arduini, D., additional, Palomino, W. A., additional, Argandona, F., additional, Kohen, P., additional, Azua, R., additional, Scarella, A., additional, Devoto, L., additional, McKinnon, B., additional, Bersinger, N. A., additional, Mueller, M. D., additional, Bonavita, M., additional, Mattila, M., additional, Ferreira, F. P., additional, Maia-Filho, V., additional, Rocha, A. M., additional, Serafini, P., additional, Motta, E. L. A., additional, Kim, H., additional, Kim, C. H., additional, You, R. M., additional, Nah, H. Y., additional, Lee, J. W., additional, Kang, H. J., additional, Kang, B. M., additional, Letur - Koenirsch, H., additional, Haouzi, D., additional, Olivennes, F., additional, Rouleau, C., additional, Cohen-Bacri, P., additional, Dechaud, H., additional, Hamamah, S., additional, D'Hooghe, T., additional, Hummelshoj, L., additional, Dunselman, G. A. J., additional, Dirksen, C. D., additional, EndoCost Consortium, W. E. R. F., additional, Simoens, S., additional, Novembri, R., additional, Luisi, S., additional, Carrarelli, P., additional, Rocha, A. L. L., additional, Toti, P., additional, Reis, F. M., additional, Florio, P., additional, Petraglia, F., additional, Bruce, K. D., additional, Sadek, K. H., additional, Macklon, N., additional, Cagampang, F. R., additional, Cheong, Y., additional, Goudakou, M., additional, Kalogeraki, A., additional, Matalliotakis, I., additional, Papatheodorou, A., additional, Pasadaki, T., additional, Karkanaki, A., additional, Prapas, I., additional, Panagiotidis, I., additional, Kasapi, E., additional, Barlow, D., additional, Oliver, J., additional, Loumaye, E., additional, Khanmohammadi, M., additional, kazemnejad, S., additional, darzi, S., additional, Khanjani, S., additional, Zarnani, A., additional, Akhondi, M., additional, Tan, C. W., additional, Ng, C. P., additional, Loh, S. F., additional, Tan, H. H., additional, Choolani, M., additional, Griffith, L., additional, Chan, J., additional, Andersson, K. L., additional, Sundqvist, J., additional, Scarselli, G., additional, Gemzell-Danielsson, K., additional, Lalitkumar, P. G., additional, Jana, S., additional, Chattopadhyay, R., additional, Datta Ray, C., additional, Chaudhury, K., additional, Chakravarty, B. N., additional, Hannan, N., additional, Evans, J., additional, Hincks, C., additional, Rombauts, L. J. F., additional, Salamonsen, L. A., additional, Choi, D., additional, Lee, J., additional, Park, J., additional, Chang, H., additional, Kim, M., additional, Hwang, K., additional, Takeuchi, K., additional, Kurematsu, T., additional, Fukumoto, Y., additional, Yuki, Y., additional, Kuroki, Y., additional, Homan, Y., additional, Sata, Y., additional, Takeuchi, M., additional, Munoz Munoz, E., additional, Ortiz Olivera, G., additional, Fernandez Lopez, I., additional, Martinez Martinez, B., additional, Aguilar Prieto, J., additional, Portela Perez, S., additional, Pellicer Martinez, A., additional, Keltz, M., additional, Sauerbrun, M., additional, Breborowicz, A., additional, Gonzales, E., additional, Vicente-Munoz, S., additional, Puchades-Carrasco, L., additional, Morcillo, I., additional, Hidalgo, J. J., additional, Gilabert-Estelles, J., additional, Novella-Maestre, E., additional, Pellicer, A., additional, Pineda-Lucena, A., additional, Yavorovskaya, K. A., additional, Okhtyrskaya, T. A., additional, Demura, T. A., additional, Faizulina, N. M., additional, Ezhova, L. S., additional, Kogan, E. A., additional, Bilibio, J. P., additional, Souza, C. A. B., additional, Rodini, G. P., additional, Genro, V., additional, Andreoli, C. G., additional, de Conto, E., additional, Cunha-Filho, J. S. L., additional, Saare, M., additional, Soritsa, D., additional, Jarva, L., additional, Vaidla, K., additional, Palta, P., additional, Laan, M., additional, Karro, H., additional, Soritsa, A., additional, Salumets, A., additional, Peters, M., additional, Miskova, A., additional, Pilmane, M., additional, Rezeberga, D., additional, Assou, S., additional, Letur, H., additional, Piomboni, P., additional, Stendardi, A., additional, Gambera, L., additional, De Leo, V., additional, Focarelli, R., additional, Tamm, K., additional, Simm, J., additional, Metsis, M., additional, Vodolazkaia, A., additional, Fassbender, A., additional, Kyama, C. M., additional, Bokor, A., additional, Schols, D., additional, Huskens, D., additional, Meuleman, C., additional, Peeraer, K., additional, Tomassetti, C., additional, D'Hooghe, T. M., additional, Machens, K., additional, Afhuppe, W., additional, Schulz, A., additional, Diefenbach, K., additional, Schutt, B., additional, Faustmann, T., additional, Reischl, J., additional, Altmae, S., additional, Reimand, J., additional, Laisk, T., additional, Hovatta, O., additional, Kolde, R., additional, Vilo, J., additional, Stavreus-Evers, A., additional, Lee, J. H., additional, Kim, S. G., additional, Kim, Y. Y., additional, Park, I. H., additional, Sun, H. G., additional, Lee, K. H., additional, Ezoe, K., additional, Kawano, H., additional, Yabuuchi, A., additional, Ochiai, K., additional, Nagashima, H., additional, Osada, H., additional, Kagawa, N., additional, Kato, O., additional, Tamura, I., additional, Asada, H., additional, Taketani, T., additional, Tamura, H., additional, Sugino, N., additional, Garcia Velasco, J., additional, Prieto, L., additional, Quesada, J. F., additional, Cambero, O., additional, Toribio, M., additional, Hur, C. Y., additional, Lim, K. S., additional, Lee, W. D., additional, Lim, J. H., additional, Germeyer, A., additional, Nelson, L., additional, Graham, A., additional, Jauckus, J., additional, Strowitzki, T., additional, Lessey, B., additional, Gyulmamedova, I., additional, Illina, O., additional, Illin, I., additional, Mogilevkina, I., additional, Chaika, A., additional, Nosenko, O., additional, Boykova, I., additional, Gulmamedova, E., additional, Isik, H., additional, Moraloglu, O., additional, Seven, A. L. I., additional, Kilic, S., additional, Erkayiran, U., additional, Caydere, M., additional, Batioglu, S., additional, Alhalabi, M., additional, Samawi, S., additional, Taha, A., additional, Kafri, N., additional, Modi, S., additional, Khatib, A., additional, Sharif, J., additional, Othman, A., additional, Lancuba, S., additional, Branzini, C., additional, Lopez, M., additional, Baricalla, A., additional, Cristina, C., additional, Chen, J., additional, Jiang, Y., additional, Zhen, X., additional, Hu, Y., additional, Yan, G., additional, Sun, H., additional, Mizumoto, J., additional, Ueno, J., additional, Carvalho, F. M., additional, Casals, G., additional, Ordi, J., additional, Guimera, M., additional, Creus, M., additional, Fabregues, F., additional, Casamitjana, R., additional, Carmona, F., additional, Balasch, J., additional, Choi, Y. S., additional, Kim, K. C., additional, Kim, K. H., additional, Lee, B. S., additional, Kim, S. H., additional, Overbergh, L., additional, Verdrengh, E., additional, Kyama, C., additional, Waelkens, E., additional, Mathieu, C., additional, Iwasa, T., additional, Hatano, K., additional, Hasegawa, E., additional, Ito, H., additional, Isaka, K., additional, L. Rocha, A. L., additional, Reis, F., additional, Lee, K. S., additional, Joo, J. K., additional, Son, J. B., additional, Choi, J. R., additional, Vidali, A., additional, Barad, D. H., additional, Gleicher, N., additional, Sayyah-Melli, M., additional, and Kazemi-Shishvan, M., additional

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2011
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42. POSTER VIEWING SESSION - MALE AND FEMALE FERTILITY PRESERVATION

Author

Akakubo, N., primary, Kagawa, N., additional, Yabuuchi, A., additional, Silber, S. J., additional, Yamaguchi, S., additional, Nagumo, Y., additional, Takai, Y., additional, Ishihara, S., additional, Takehara, Y., additional, Kato, O., additional, Kocent, J., additional, Hu, J. C. Y., additional, Neri, Q. V., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Armuand, G., additional, Rodriguez-Wallberg, K., additional, Wettergren, L., additional, Lampic, C., additional, Martinez-Soto, J. C., additional, Domingo, J. C., additional, Cordovilla, B., additional, Gadea, J., additional, Landeras, J., additional, Sadri-Ardekani, H., additional, Akhondi, M. M., additional, van der Veen, F., additional, de Rooij, D. G., additional, Repping, S., additional, van Pelt, A. M. M., additional, Vanacker, J., additional, Luyckx, V., additional, Dolmans, M. M., additional, Amorim, C. A., additional, Van Langendonckt, A., additional, Donnez, J., additional, Camboni, A., additional, Gavella, M., additional, Lipovac, V., additional, Siftar, Z., additional, Garaj-Vrhovac, V., additional, Gajski, G., additional, Gook, D., additional, Borg, J., additional, Edgar, D. H., additional, Brink-van der Vlugt, J. J., additional, Van der Velden, V. H. J., additional, Noordijk, A., additional, Timmer-Bosscha, H., additional, Tissing, W. J. E., additional, Land, J. A., additional, Hollema, H., additional, Van Echten-Arends, J., additional, Alvarez, J. G., additional, Gosalvez, A., additional, Velilla, E., additional, Lopez-Teijon, M., additional, Lopez-Fernandez, C., additional, Gosalvez, J., additional, Kristensen, S. G., additional, Rasmussen, A., additional, Yding Andersen, C., additional, Raziel, A., additional, Friedler, S., additional, Gidoni, Y., additional, Ben Ami, I., additional, Kaufman, S., additional, Omansky, A., additional, Strassburger, D., additional, Komarovsky, D., additional, Bern, O., additional, Kasterstein, E., additional, Komsky, A., additional, Maslansky, B., additional, Ron-El, R., additional, Fujimoto, A., additional, Osuga, Y., additional, Ichinose, M., additional, Oishi, H., additional, Harada, M., additional, Koizumi, M., additional, Takemura, Y., additional, Yano, T., additional, Taketani, Y., additional, Molnar, Z., additional, Mokanszki, A., additional, Benyo, M., additional, Bazsane Kassai, Z., additional, Olah, E., additional, Jakab, A., additional, Rodriguez-Wallberg, K. A., additional, Vonheim, E., additional, Gumus, E., additional, Persson, I., additional, Lundqvist, M., additional, Karlstrom, P. O., additional, Hovatta, O., additional, Pasqualotto, F. F., additional, Teixeira, R., additional, Medeiros, G. S., additional, Canabarro, C., additional, Tonezer, J., additional, Grando, A. P. C., additional, Borges Jr., E., additional, Pasqualotto, E. B., additional, Westphal, J. R., additional, Bastings, L., additional, Beerendonk, C. C. M., additional, Braat, D. D. M., additional, Peek, R., additional, Courbiere, B., additional, Berthelot-Ricou, A., additional, Di Giorgio, C., additional, De Meo, M., additional, Roustan, A., additional, Botta, A., additional, Perrin, J., additional, Abir, R., additional, Orvieto, R., additional, Friedman, O., additional, Ben-Haroush, A., additional, Fisch, B., additional, Lawrenz, B., additional, Henes, J., additional, Henes, M., additional, Neunhoeffer, E., additional, Schmalzing, M., additional, Fehm, T., additional, and Koetter, I., additional

Published
2011
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44. Session 65: Fertility Preservation 3

Author

Chian, R. C., primary, Yan, J., additional, Suzuki, J. J., additional, Yu, X. M., additional, Wang, J., additional, Tan, S. L., additional, Kan, F. W. K., additional, Liu, P., additional, Qiao, J., additional, Vanderzwalmen, P., additional, Wirleitner, B., additional, Stecher, A., additional, Lejeune, B., additional, Puissant, F., additional, Vanderzwalmen, S., additional, Schwerda, D., additional, Baramsai, B., additional, Zech, N. H., additional, Antinori, M., additional, Licata, E., additional, Dani, G., additional, Cerusico, F., additional, Versaci, C., additional, Antinori, S., additional, Menezes, J., additional, Sjoblom, P., additional, Brask, K., additional, Nordqvist, S., additional, Wramsby, H., additional, Rodriguez-Wallberg, K. A., additional, Borgstrom, B., additional, Sheikhi, M., additional, Lundqvist, M. L., additional, Hovatta, O., additional, Trapphoff, T., additional, El Hajj, N., additional, Haaf, T., additional, Eichenlaub-Ritter, U., additional, Gomes, C., additional, Alegretti, J. R., additional, Rocha, A. M., additional, Motta, E. L. A., additional, Serafini, P., additional, Hassun, P. A., additional, Baracat, E. C., additional, and Smith, G. D., additional

Published
2010
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46. Co-existence of heparin-binding epidermal growth factorlike growth factor and pinopodes in human endometrium at the time of implantation

Author

Stavreus-Evers, A., Aghajanova, L., Brismar, Hjalmar, Eriksson, H., Landgren, B. M., Hovatta, O., Stavreus-Evers, A., Aghajanova, L., Brismar, Hjalmar, Eriksson, H., Landgren, B. M., and Hovatta, O.

Abstract

Pinopodes have been suggested to be markers of uterine receptivity. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is expressed in increasing amounts in the secretory phase endometrium and is considered to be important for the human implantation process. The aim of this study was to investigate a possible co-existence of pinopodes and HB-EGF in the normal human endometrium. Endometrial biopsies were obtained from women with normal menstrual cycles. The biopsies were examined by scanning electron microscopy for the detection of pinopodes, by immunohistochemistry for the expression of HB-EGF protein, and by confocal microscopy to determine if HB-EGF was present on the surface of the pinopodes. The expression of HB-EGF in luminal and glandular epithelium was highest when fully developed pinopodes were present. Using confocal microscopy it was shown that HB-EGF was present both inside the luminal epithelial cells and on the surface of pinopodes. These findings suggest that HB-EGF might play a role in both the attachment and penetration steps in the human implantation process. Furthermore, the immunohistochemical staining demonstrates that HB-EGF can be used as a marker for the implantation window., QC 20100525

Published
2002
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48. Immortalized human skin fibroblast feeder cells support growth and maintenance of both human embryonic and induced pluripotent stem cells

Author

Unger, C., primary, Gao, S., additional, Cohen, M., additional, Jaconi, M., additional, Bergstrom, R., additional, Holm, F., additional, Galan, A., additional, Sanchez, E., additional, Irion, O., additional, Dubuisson, J. B., additional, Giry-Laterriere, M., additional, Salmon, P., additional, Simon, C., additional, Hovatta, O., additional, and Feki, A., additional

Published
2009
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