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47 results on '"Horcajadas, J. A."'

1. Development of a Novel Non-invasive Metabolomics Assay to Predict Implantation Potential of Human Embryos

Author: Cabello-Pinedo, S., Abdulla, H., Mas, S., Fraire, A., Maroto, B., Seth-Smith, M., Escriba, M., Teruel, J., Crespo, J., Munné, S., and Horcajadas, J. A.
Published: 2024
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2. Development of a new comprehensive and reliable endometrial receptivity map (ER Map/ER Grade) based on RT-qPCR gene expression analysis

Author: Enciso, M, Carrascosa, J P, Sarasa, J, Martínez-Ortiz, P A, Munné, S, Horcajadas, J A, and Aizpurua, J
Published: 2018
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3. The mid-secretory endometrial transcriptomic landscape in endometriosis: a meta-analysis

Author: Vargas, E, primary, García-Moreno, E, additional, Aghajanova, L, additional, Salumets, A, additional, Horcajadas, J A, additional, Esteban, F J, additional, and Altmäe, S, additional
Published: 2022
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4. In Vitro Culture of Mouse Embryos Reduces Differential Gene Expression Between Inner Cell Mass and Trophectoderm

Author: Giritharan, G., Delle Piane, L., Donjacour, A., Esteban, F. J., Horcajadas, J. A., Maltepe, E., and Rinaudo, P.
Published: 2012
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5. P–171 Automated oocyte and zygote denudation using a novel microfluidic device supervised by a computer vision algorithm

Author: Guerrer. Sánchez, J, primary, Cabello, Y, additional, Fernánde. Blanco, G, additional, Fidalgo, J, additional, Hernánde. Montilla, I, additional, Carasa, P, additional, Matthys, L, additional, Belchin, P, additional, Horcajadas, J A, additional, and Munné, S, additional
Published: 2021
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6. P–403 Sodium tungstate increases embryo adhesion through a direct effect on endometrial cells

Author: Canals, I, primary, Cotán, D, additional, Torres, R, additional, Horcajadas, J A, additional, and Arbat, A, additional
Published: 2021
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7. Endometrial Receptivity Arrays (ERA) with different luteal support protocols after triggering final oocyte maturation with GnRH agonist: O-242

Author: Bermejo, A., Cerrillo, M., Ortega, I., Martínez-Conejero, J. A., Ruiz-Alonso, M., Horcajadas, J. A., Simón, C., and García-Velasco, J. A.
Published: 2012

8. Endometrial gene expression analysis in infertile women in natural and hormone replacement cycles: O-243

Author: Altmäe, S., Martinez-Conejero, J. A., Esteban, F. J., Horcajadas, J. A., Salumets, A., and Stavreus-Evers, A.
Published: 2012

9. mid-secretory endometrial transcriptomic landscape in endometriosis: a meta-analysis.

Author: Vargas, E, García-Moreno, E, Aghajanova, L, Salumets, A, Horcajadas, J A, Esteban, F J, and Altmäe, S
Subjects: ENDOMETRIOSIS, GENE expression, META-analysis
Abstract: STUDY QUESTION Do women with endometriosis have a different endometrial gene expression profile at the time of embryo implantation than women without endometriosis? SUMMARY ANSWER The endometrial gene expression profile of women with endometriosis differs from that of women without endometriosis at the mid-secretory phase, although the differences are small. WHAT IS KNOWN ALREADY About 50% of women with endometriosis suffer infertility. Several molecular studies have suggested impaired endometrial receptivity in women with endometriosis, while others have detected no dysregulation of endometrial receptivity. Nevertheless, the previous endometrial transcriptome studies comparing women with and without endometriosis have been performed in small sample size with limited statistical power. We set out to systematically search and compile data of endometrial gene expression signatures at the receptive phase in women with endometriosis versus control women. Based on the obtained data, we conducted a meta-analysis of differentially expressed genes in order to raise the power of the analysis for identifying the molecular profiles of receptive phase endometria in endometriosis. STUDY DESIGN, SIZE, DURATION A systematic literature search was conducted up to February 2022 following PRISMA criteria and included PubMed, Cochrane and Web of Science databases. For the systematic search, the term 'endometriosis' was paired with the terms 'transcriptomics', 'transcriptome', 'gene expression', 'RNA-seq', 'sequencing' and 'array', by using the Boolean operator 'AND' to connect them. Articles written in English were screened and interrogated for data extraction. PARTICIPANTS/MATERIALS, SETTING, METHODS A meta-analysis was performed on the selected studies to extract the differentially expressed genes described at the mid-secretory phase in women with endometriosis versus women without endometriosis in natural cycles, using the robust rank aggregation method. In total, transcriptome data of 125 women (78 patients and 47 controls) were meta-analysed, with a special focus on endometrial receptivity-specific genes based on commercial endometrial receptivity tests. MAIN RESULTS AND THE ROLE OF CHANCE In total, 8 studies were eligible for the quantitative meta-analysis, gathering transcriptome data from the mid-secretory phase endometria of 125 women. A total of 7779 differentially expressed transcripts between the study groups were retrieved (3496 up-regulated and 4283 down-regulated) and were meta-analysed. After stringent multiple correction, there was no differential expression of any single molecule in the endometrium of women with endometriosis versus controls, while enrichment analysis detected that the pathways of chemotaxis and locomotion are dysregulated in endometriosis. Further analysis of endometrial receptivity-specific genes highlighted dysregulation of C4BPA , MAOA and PAEP and enrichment of immune and defence pathways in women with endometriosis. LIMITATIONS, REASONS FOR CAUTION Most of the studies included into the meta-analysis were relatively small and had different study designs, which might have contributed to a bias. WIDER IMPLICATIONS OF THE FINDINGS The current meta-analysis supports the hypothesis that endometrial receptivity is altered in women with endometriosis, although the changes are small. The molecules and pathways identified could serve as future biomarkers and therapeutical targets in detecting and treating endometriosis-associated infertility. STUDY FUNDING/COMPETING INTEREST(S) The authors declare no competing interests. This work was supported by the Spanish Ministry of Education, Culture and Sport [grant FPU15/01193] and the Margarita Salas program for the Requalification of the Spanish University system [grant UJAR01MS]; Spanish Ministry of Economy, Industry and Competitiveness (MINECO) and European Regional Development Fund (FEDER): grants RYC-2016-21199 and ENDORE SAF2017-87526-R; Programa Operativo FEDER Andalucía (B-CTS-500-UGR18; A-CTS-614-UGR20); the Junta de Andalucía [BIO-302; and PAIDI P20_00158]; the University of Jaén [PAIUJA-EI_CTS02_2017]; the University of Granada, Plan Propio de Investigación 2016, Excellence actions: Units of Excellence; Unit of Excellence on Exercise and Health (UCEES), and by the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades and European Regional Development Fund (ERDF), ref. SOMM17/6107/UGR; the Estonian Research Council (grant PRG1076); Horizon 2020 innovation (ERIN, grant no. EU952516) of the European Commission and Enterprise Estonia (grant EU48695). TRIAL REGISTRATION NUMBER The systematic review was registered at PROSPERO (identifier: CRD42020122054). [ABSTRACT FROM AUTHOR]
Published: 2022
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10. Effect of an Intrauterine Device on the Gene Expression Profile of the Endometrium

Author: Horcajadas, J A., Sharkey, A M., Catalano, R D., Sherwin, J R. A., Domínguez, F, Burgos, L A., Castro, A, Peraza, M R., Pellicer, A, and Simón, C
Published: 2006

11. Transcriptome of early embryonic invasion at implantation sites in a murine model

Author: Moreno-Moya, J. M., primary, Franchi, N. A., additional, Martínez-Escribano, S., additional, Martínez-Conejero, J. A., additional, Bocca, S., additional, Oehninger, S., additional, and Horcajadas, J. A., additional
Published: 2016
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12. Endometrial gene expression analysis in infertile women in natural and hormone replacement cycles

Author: Altmael, S., Martinez-Conejero, J. A., Esteban, F. J., Horcajadas, J. A., Salumets, A., Stavreus-Evers, Anneli, Altmael, S., Martinez-Conejero, J. A., Esteban, F. J., Horcajadas, J. A., Salumets, A., and Stavreus-Evers, Anneli
Published: 2012

13. Endometrial gene expression analysis at the time of embryo implantation in women with unexplained infertility

Author: Altmäe, Signe, Martínez-Conejero, J. A., Salumets, A., Simón, C., Horcajadas, J. A., Stavreus-Evers, Anneli, Altmäe, Signe, Martínez-Conejero, J. A., Salumets, A., Simón, C., Horcajadas, J. A., and Stavreus-Evers, Anneli
Abstract: Successful embryo implantation depends on the quality of the embryo, as well as on the receptivity of the endometrium. The aim of this study was to investigate the endometrial gene expression profile in women with unexplained infertility in comparison with fertile controls at the time of embryo implantation in order to find potential predictive markers of uterine receptivity and to identify the molecular mechanisms of infertility. High-density oligonucleotide gene arrays, comprising 44 000 gene targets, were used to define the endometrial gene expression profile in infertile (n = 4) and fertile (n = 5) women during the mid-secretory phase (day LH +7). Microarray results were validated using real-time PCR. Analyses of expression data were carried out using non-parametric methods. Hierarchical clustering and principal component analysis showed a clear distinction in endometrial gene expression between infertile and fertile women. In total we identified 145 significantly (>3-fold change) up-regulated and 115 down-regulated genes in infertile women versus controls. Via Database for Annotation, Visualization and Integrated Discovery functional analysis we detected a substantial number of dysregulated genes in the endometria of infertile women, involved in cellular localization (21.1%) and transport (18.8%) and transporter activity (13.1%) and with major localization in extracellular regions (19.2%). Ingenuity Pathways Analysis of the gene list showed dysregulation of gene pathways involved in leukocyte extravasation signalling, lipid metabolism and detoxification in the endometria of infertile women. In conclusion, endometrial gene expression in women with unexplained infertility at the time of embryo implantation is markedly different from that in fertile women. These results provide new information on genes and pathways that may have functional significance as regards to endometrial receptivity and subsequent embryo implantation.
Published: 2010
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14. Predicting adverse obstetric outcome after early pregnancy events and complications: a review

Author: van Oppenraaij, R H F, Jauniaux, E, Christiansen, O B, Horcajadas, J A, Farquharson, R G, Exalto, N, van Oppenraaij, R H F, Jauniaux, E, Christiansen, O B, Horcajadas, J A, Farquharson, R G, and Exalto, N
Abstract: BACKGROUND The aim was to evaluate the impact of early pregnancy events and complications as predictors of adverse obstetric outcome. METHODS We conducted a literature review on the impact of first trimester complications in previous and index pregnancies using Medline and Cochrane databases covering the period 1980-2008. RESULTS Clinically relevant associations of adverse outcome in the subsequent pregnancy with an odds ratio (OR) > 2.0 after complications in a previous pregnancy are the risk of perinatal death after a single previous miscarriage, the risk of very preterm delivery (VPTD) after two or more miscarriages, the risk of placenta praevia, premature preterm rupture of membranes, VPTD and low birthweight (LBW) after recurrent miscarriage and the risk of VPTD after two or more termination of pregnancy. Clinically relevant associations of adverse obstetric outcome in the ongoing pregnancy with an OR > 2.0 after complications in the index pregnancy are the risk of LBW and very low birthweight (VLBW) after a threatened miscarriage, the risk of pregnancy-induced hypertension, pre-eclampsia, placental abruption, preterm delivery (PTD), small for gestational age and low 5-min Apgar score after detection of an intrauterine haematoma, the risk of VPTD and intrauterine growth restriction after a crown-rump length discrepancy, the risk of VPTD, LBW and VLBW after a vanishing twin phenomenon and the risk of PTD, LBW and low 5-min Apgar score in a pregnancy complicated by severe hyperemesis gravidarum. CONCLUSIONS Data from our literature review indicate, by finding significant associations, that specific early pregnancy events and complications are predictors for subsequent adverse obstetric and perinatal outcome. Though, some of these associations are based on limited or small uncontrolled studies. Larger population-based controlled studies are needed to confirm these findings. Nevertheless, identification of these risks will improve obstetric care.
Published: 2009

15. Endometrial receptivity and implantation are not affected by the presence of uterine intramural leiomyomas : A clinical and functional genomics analysis

Author: Horcajadas, J A, Goyri, E, Higon, M A, Martinez-Conejero, J A, Gambadauro, Pietro, Garcia, G, Meseguer, M, Simon, C, Pellicer, A, Horcajadas, J A, Goyri, E, Higon, M A, Martinez-Conejero, J A, Gambadauro, Pietro, Garcia, G, Meseguer, M, Simon, C, and Pellicer, A
Published: 2008
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16. Endometriosis, endometrium, implantation and fallopian tube

Author: Tan, C. W., primary, Lee, Y. H., additional, Choolani, M., additional, Tan, H. H., additional, Griffith, L., additional, Chan, J., additional, Chuang, P. C., additional, Wu, M. H., additional, Lin, Y. J., additional, Tsai, S. J., additional, Rahmati, M., additional, Petitbarat, M., additional, Dubanchet, S., additional, Bensussan, A., additional, Chaouat, G., additional, Ledee, N., additional, Bissonnette, L., additional, Haouzi, D., additional, Monzo, C., additional, Traver, S., additional, Bringer, S., additional, Faidherbe, J., additional, Perrochia, H., additional, Ait-Ahmed, O., additional, Dechaud, H., additional, Hamamah, S., additional, Ibrahim, M. G., additional, de Arellano, M. L. B., additional, Sachtleben, M., additional, Chiantera, V., additional, Frangini, S., additional, Younes, S., additional, Schneider, A., additional, Plendl, J., additional, Mechsner, S., additional, Ono, M., additional, Hamai, H., additional, Chikawa, A., additional, Teramura, S., additional, Takata, R., additional, Sugimoto, T., additional, Iwahashi, K., additional, Ohhama, N., additional, Nakahira, R., additional, Shigeta, M., additional, Park, I. H., additional, Lee, K. H., additional, Sun, H. G., additional, Kim, S. G., additional, Lee, J. H., additional, Kim, Y. Y., additional, Kim, H. J., additional, Jeon, G. H., additional, Kim, C. M., additional, Bocca, S., additional, Wang, H., additional, Anderson, S., additional, Yu, L., additional, Horcajadas, J., additional, Oehninger, S., additional, Bastu, E., additional, Mutlu, M. F., additional, Celik, C., additional, Yasa, C., additional, Dural, O., additional, Buyru, F., additional, Quintana, F., additional, Cobo, A., additional, Remohi, J., additional, Ferrando, M., additional, Matorras, R., additional, Bermejo, A., additional, Iglesias, C., additional, Cerrillo, M., additional, Ruiz, M., additional, Blesa, D., additional, Simon, C., additional, Garcia-Velasco, J. A., additional, Chamie, L., additional, Ribeiro, D. M. F., additional, Riboldi, M., additional, Pereira, R., additional, Rosa, M. B., additional, Gomes, C., additional, de Mello, P. H., additional, Fettback, P., additional, Domingues, T., additional, Cambiaghi, A., additional, Soares, A. C. P., additional, Kimati, C., additional, Motta, E. L. A., additional, Serafini, P., additional, Hapangama, D. K., additional, Valentijn, A. J., additional, Al-Lamee, H., additional, Palial, K., additional, Drury, J. A., additional, von Zglinicki, T., additional, Saretzki, G., additional, Gargett, C. E., additional, Liao, C. Y., additional, Sung, Y. J., additional, Li, H. Y., additional, Morotti, M., additional, Remorgida, V., additional, Venturini, P. L., additional, Ferrero, S., additional, Nabeta, M., additional, Iki, A., additional, Hashimoto, H., additional, Koizumi, M., additional, Matsubara, Y., additional, Hamada, K., additional, Fujioka, T., additional, Matsubara, K., additional, Kusanagi, Y., additional, Nawa, A., additional, Zanatta, A., additional, da Rocha, A. M., additional, Guerra, J. L., additional, Cogliati, B., additional, Bianchi, P. d. M., additional, Prieto, B., additional, Exposito, A., additional, Mendoza, R., additional, Rabanal, A., additional, Bedaiwy, M., additional, Yi, L., additional, Dahoud, W., additional, Liu, J., additional, Hurd, W., additional, Falcone, T., additional, Biscotti, C., additional, Mesiano, S., additional, Sugiyama, R., additional, Nakagawa, K., additional, Nishi, Y., additional, Kuribayashi, Y., additional, Akira, S., additional, Germeyer, A., additional, Rosner, S., additional, Jauckus, J., additional, Strowitzki, T., additional, von Wolff, M., additional, Khan, K. N., additional, Kitajima, M., additional, Fujishita, A., additional, Nakashima, M., additional, Masuzaki, H., additional, Kajihara, T., additional, Ishihara, O., additional, Brosens, J., additional, Vezmar, K., additional, Savournin, V., additional, Balet, R., additional, Loh, S. F., additional, Tannenbaum, S. R., additional, Chan, J. K. Y., additional, Scarella, A., additional, Chamy, V., additional, Devoto, L., additional, Abrao, M., additional, Sovino, H., additional, Krasnopolskaya, K., additional, Popov, A., additional, Kabanova, D., additional, Beketova, A., additional, Ivakhnenko, V., additional, Shohayeb, A., additional, Wahba, A., additional, Abousetta, A., additional, al-inany, H., additional, El Daly, A., additional, Zayed, M., additional, Kvaskoff, M., additional, Han, J., additional, Missmer, S. A., additional, Navarro, P., additional, Meola, J., additional, Ribas, C. P., additional, Paz, C. P., additional, Ferriani, R. A., additional, Donabela, F. C., additional, Tafi, E., additional, Maggiore, U. L. R., additional, Scala, C., additional, Hackl, J., additional, Strehl, J., additional, Wachter, D., additional, Dittrich, R., additional, Cupisti, S., additional, Hildebrandt, T., additional, Lotz, L., additional, Attig, M., additional, Hoffmann, I., additional, Renner, S., additional, Hartmann, A., additional, Beckmann, M. W., additional, Urquiza, F., additional, Ferrer, C., additional, Incera, E., additional, Azpiroz, A., additional, Junovich, G., additional, Pappalardo, C., additional, Guerrero, G., additional, Pasqualini, S., additional, Gutierrez, G., additional, Corti, L., additional, Sanchez, A. M., additional, Bordignon, P. P., additional, Santambrogio, P., additional, Levi, S., additional, Persico, P., additional, Vigano, P., additional, Papaleo, E., additional, Ferrari, S., additional, Candiani, M., additional, van der Houwen, L. E. E., additional, Schreurs, A. M. F., additional, Lambalk, C. B., additional, Schats, R., additional, Hompes, P. G. A., additional, Mijatovic, V., additional, Xu, S. Y., additional, Li, J., additional, Chen, X. Y., additional, Chen, S. Q., additional, Guo, L. Y., additional, Mathew, D., additional, Nunes, Q., additional, Lane, B., additional, Fernig, D., additional, Hapangama, D., additional, Lind, T., additional, Hammarstrom, M., additional, Golmann, D., additional, Rodriguez-Wallberg, K., additional, Hestiantoro, A., additional, Cakra, A., additional, Aulia, A., additional, Al-Inany, H., additional, Houston, B., additional, Farquhar, C., additional, Tagliaferri, V., additional, Gagliano, D., additional, Immediata, V., additional, Tartaglia, C., additional, Zumpano, A., additional, Campagna, G., additional, Lanzone, A., additional, Guido, M., additional, Matsuzaki, S., additional, Darcha, C., additional, Botchorishvili, R., additional, Pouly, J. L., additional, Mage, G., additional, Canis, M., additional, Shivhare, S. B., additional, Bulmer, J. N., additional, Innes, B. A., additional, Lash, G. E., additional, de Graaff, A. A., additional, Zandstra, H., additional, Smits, L. J., additional, Van Beek, J. J., additional, Dunselman, G. A. J., additional, Bozdag, G., additional, Calis, P. T., additional, Demiralp, D. O., additional, Ayhan, B., additional, Igci, N., additional, Yarali, H., additional, Acar, N., additional, Er, H., additional, Ozmen, A., additional, Ustunel, I., additional, Korgun, E. T., additional, Kuroda, K., additional, Kuroda, M., additional, Arakawa, A., additional, Kitade, M., additional, Brosens, A. I., additional, Brosens, J. J., additional, Takeda, S., additional, and Yao, T., additional
Published: 2013
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17. Effect of ICSI on gene expression and development of mouse preimplantation embryos

Author: Giritharan, G., primary, Li, M. W., additional, Di Sebastiano, F., additional, Esteban, F. J., additional, Horcajadas, J. A., additional, Lloyd, K. C. K., additional, Donjacour, A., additional, Maltepe, E., additional, and Rinaudo, P. F., additional
Published: 2012
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18. A comparison of array technologies and next generation sequencing technologies in preconception genetic diagnosis

Author: Bisignano, A., primary, Ketterson, K., additional, Fischer, J., additional, Wells, D., additional, Horcajadas, J., additional, and Bush, E., additional
Published: 2012
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19. REPRODUCTIVE (EPI) GENETICS

Author: Sertyel, S., primary, Kolankaya, A., additional, Yigit, A., additional, Cengiz, F., additional, Kunacaf, G., additional, Akman, M. A., additional, Gurgan, T., additional, Yu, B., additional, DeCherney, A., additional, Segars, J., additional, Russanova, V., additional, Howard, B., additional, Serafini, P., additional, Kimati, C., additional, Hassun, P., additional, Cuzzi, J., additional, Peres, M., additional, Riboldi, M., additional, Gomes, C., additional, Fettback, P., additional, Alegretti, J., additional, motta, E., additional, Lappa, C., additional, Ottolini, C. S., additional, Summers, M. C., additional, Sage, K., additional, Rogers, S., additional, Griffin, D. K., additional, Handyside, A. H., additional, Thornhill, A. R., additional, Ubaldi, F., additional, Capalbo, A., additional, Wright, G., additional, Elliott, T., additional, Maggiulli, R., additional, Rienzi, L., additional, Nagy, Z. P., additional, Cinar Yapan, C., additional, Beyazyurek, C., additional, Ekmekci, C. G., additional, Altin, G., additional, Yesil, M., additional, Yelke, H., additional, Kahraman, S., additional, Khalil, M., additional, Rittenberg, V., additional, Khalaf, Y., additional, El-toukhy, T., additional, Alvaro Mercadal, B., additional, Imbert, R., additional, Demeestere, I., additional, De Leener, A., additional, Englert, Y., additional, Costagliola, S., additional, Delbaere, A., additional, Zimmermann, B., additional, Ryan, A., additional, Baner, J., additional, Gemelos, G., additional, Dodd, M., additional, Rabinowitz, M., additional, Hill, M., additional, Sandalinas, M., additional, Garcia-Guixe, E., additional, Jimenez-Macedo, A., additional, Gimenez, C., additional, Wemmer, N., additional, Potter, D., additional, Keller, J., additional, Cater, E., additional, Lynch, C., additional, Jenner, L., additional, Berrisford, K., additional, Campbell, A., additional, Keown, N., additional, Rouse, H., additional, Craig, A., additional, Fishel, S., additional, Palomares, A. R., additional, Lendinez Ramirez, A. M., additional, Martinez, F., additional, Ruiz Galdon, M., additional, Reyes Engel, A., additional, Mamas, T., additional, Xanthopoulou, L., additional, Heath, C., additional, Doshi, A., additional, Serhal, P., additional, SenGupta, S. B., additional, Plaza, S., additional, Templin, C., additional, Saguet, F., additional, Claustres, M., additional, Girardet, A., additional, Biricik, A., additional, Colamaria, S., additional, Bono, S., additional, Spizzichino, L., additional, Fiorentino, F., additional, Alegretti, J. R., additional, Barros, B., additional, Motta, E. L. A., additional, Tulay, P., additional, Naja, R. P., additional, Cascales-Roman, O., additional, Cawood, S., additional, Montjean, D., additional, Ravel, C., additional, Belloc, S., additional, Cohen-Bacrie, P., additional, Bashamboo, A., additional, McElreavey, K., additional, Benkhalifa, M., additional, Filippini, G., additional, Radovanovic, J., additional, Spalvieri, S., additional, Marabella, D., additional, Timperi, P., additional, Suter, T., additional, Jemec, M., additional, Traversa, M., additional, Marshall, J., additional, Leigh, D., additional, McArthur, S., additional, Zhang, L., additional, Yilmaz, A., additional, Zhang, X. Y., additional, Son, W. Y., additional, Holzer, H., additional, Ao, A., additional, Horcajadas, J. A., additional, Munne, S., additional, Fisher, J., additional, Ketterson, K., additional, Wells, D., additional, Bisignano, A., additional, Rubio, C., additional, Mateu, E., additional, Milan, M., additional, Mercader, A., additional, Bosch, E., additional, Labarta, E., additional, Crespo, J., additional, Remohi, J., additional, Simon, C., additional, Pellicer, A., additional, Garrido, N., additional, Buendia, P., additional, Delgado, A., additional, Escrich, L., additional, Poo, M. E., additional, Held, K., additional, Baukloh, V., additional, Arps, S., additional, Wittmann, S. T., additional, Petrussa, L., additional, Van de Velde, H., additional, De Rycke, M., additional, Ajredin, N., additional, Tac, H. A., additional, Yelke, H. K., additional, Basile, N., additional, Bronet, F., additional, Nogales, M. C., additional, Ariza, M., additional, Martinez, E., additional, Linan, A., additional, Gaytan, A., additional, Meseguer, M., additional, Christopikou, D., additional, Tsorva, E., additional, Economou, K., additional, Davies, S., additional, Mastrominas, M., additional, Avo Santos, M., additional, M. Lens, S., additional, C. Fauser, B., additional, S. E. Laven, J., additional, B. Baart, E., additional, Nakano, T., additional, Akamatsu, Y., additional, Sato, M., additional, Hashimoto, S., additional, Maezawa, T., additional, Himeno, T., additional, Ohnishi, Y., additional, Inoue, T., additional, Ito, K., additional, Nakaoka, Y., additional, Morimoto, Y., additional, Al Sharif, J., additional, Alhalabi, M., additional, Abou Alchamat, G., additional, Madania, A., additional, Khatib, A., additional, Kinj, M., additional, Monem, F., additional, Mahayri, Z., additional, Ajlouni, A., additional, Othman, A., additional, Chung, J. T., additional, Tan, S. L., additional, Burnik Papler, T., additional, Fon Tacer, K., additional, Devjak, R., additional, Juvan, P., additional, Virant-Klun, I., additional, Vrtacnik Bokal, E., additional, Zheng, H. Y., additional, Chen, S. L., additional, Chen, X., additional, Tang, Y., additional, Li, L., additional, Ye, D. S., additional, Yang, X. H., additional, Eichenlaub-Ritter, U., additional, Trapphoff, T., additional, Hastreiter, S., additional, Haaf, T., additional, Asada, H., additional, Maekawa, R., additional, Tamura, I., additional, Tamura, H., additional, Sugino, N., additional, Zakharova, E., additional, Zaletova, V., additional, Krivokharchenko, I., additional, Ata, B., additional, Kaplan, B., additional, Danzer, H., additional, Glassner, M., additional, and Opsahl, M., additional
Published: 2012
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20. SESSION 62: FEMALE REPRODUCTION TRACT (DYS)FUNCTION

Author: Liu, B., primary, Li, J., additional, Li, M. T., additional, Hu, K. H., additional, Xia, T. T., additional, Xu, S. Y., additional, Sadek, K., additional, Bruce, K., additional, Macklon, N., additional, Cagampang, F., additional, Cheong, Y., additional, Karasu, T., additional, Marczylo, T. H., additional, Fonseca, B. M., additional, Correia-da Silva, G., additional, Teixeira, N. A., additional, Konje, J. C., additional, Pustovrh, C., additional, Villarroel, C., additional, Arriagada, C., additional, Munoz, A., additional, Kohen, P., additional, Nestler, J. E., additional, Devoto, L., additional, Bermejo, A., additional, Cerrillo, M., additional, Ortega, I., additional, Martinez-Conejero, J. A., additional, Ruiz-Alonso, M., additional, Horcajadas, J. A., additional, Simon, C., additional, Garcia-Velasco, J. A., additional, Altmae, S., additional, Esteban, F. J., additional, Salumets, A., additional, Stavreus-Evers, A., additional, Ozornek, H., additional, Ozay, A., additional, and Ergin, E. G., additional
Published: 2012
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21. Endometrial Receptivity Is Affected in Women With High Circulating Progesterone Levels at the End of the Follicular Phase: A Functional Genomics Analysis

Author: Labarta, E., primary, Martínez-Conejero, J. A., additional, Alamá, P., additional, Horcajadas, J. A., additional, Pellicer, A., additional, Simón, C., additional, and Bosch, E., additional
Published: 2011
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22. A novel model of human implantation: 3D endometrium-like culture system to study attachment of human trophoblast (Jar) cell spheroids

Author: Wang, H., primary, Pilla, F., additional, Anderson, S., additional, Martinez-Escribano, S., additional, Herrer, I., additional, Moreno-Moya, J. M., additional, Musti, S., additional, Bocca, S., additional, Oehninger, S., additional, and Horcajadas, J. A., additional
Published: 2011
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23. SELECTED ORAL COMMUNICATION SESSION, SESSION 62: ENDOMETRIUM, IMPLANTATION AND ENDOMETRIOSIS Wednesday 6 July 2011 10:00 - 11:45

Author: Edgar, D. H., primary, de Graaff, A. A., additional, D'Hooghe, T. M., additional, Dunselman, G. A. J., additional, Dirksen, C. D., additional, Hummelshoj, L., additional, EndoCost Consortium, W. E. R. F., additional, Simoens, S., additional, Garcia-Cerrudo, E., additional, Martinez-Conejero, J. A., additional, Herrero, L., additional, Rodriguez, S., additional, Horcajadas, J. A., additional, Remohi, J., additional, Garcia-Velasco, J. A., additional, Salker, M. S., additional, Christian, M., additional, Steel, J. H., additional, Nautiyal, J., additional, Lang, F., additional, Brosens, J. J., additional, Mitra, A., additional, Bhattacharya, J., additional, Kundu, S., additional, Pal, M., additional, Mukhopadhyay, D., additional, Komsky, A., additional, Ben-Ami, I., additional, Strassburger, D., additional, Kasterstein, E., additional, Komarovsky, D., additional, Bern, O., additional, Maslansky, B., additional, Raziel, A., additional, Friedler, S., additional, Gidoni, Y. S., additional, Ron-El, R., additional, Finas, D., additional, Hunold, P., additional, Koester, F., additional, Altevogt, P., additional, and Hornung, D., additional
Published: 2011
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24. SELECTED ORAL COMMUNICATION SESSION, SESSION 70: ANDROLOGY AND SEMINAL FACTORS Wednesday 6 July 2011 14:00 - 15:45

Author: Caballero Peregrin, P., primary, Nunez-Calonge, R., additional, Guijarro, J. A., additional, Ortega, L., additional, Cortes, S., additional, Gosalvez, J., additional, Palini, S., additional, De Stefani, S., additional, Scala, V., additional, Benedetti, S., additional, Tagliamonte, M. C., additional, Catalani, S., additional, Primiterra, M. A., additional, Polli, V., additional, Rocchi, P., additional, Tiezzi, A., additional, Donati, L., additional, Pelosi, E., additional, Canestrari, F., additional, Bulletti, C., additional, Garcia-Herrero, S., additional, Meseguer, M., additional, Martinez-Conejero, J. A., additional, Romany, L., additional, Ruiz, M., additional, Horcajadas, J. A., additional, Pellicer, A., additional, Garrido, N., additional, Ramon, O., additional, Corcostegui, B., additional, Crisol, L., additional, Exposito, A., additional, Mugica, J., additional, Matorras, R., additional, Kyurkchiev, S., additional, Dyulgerova-Nikolova, D., additional, Milachich, T., additional, Shterev, A., additional, Pons Mallol, I., additional, Cercas Duque, R., additional, Villas Martin, C., additional, Brana Pelayo, C., additional, Fernandez Shaw, S., additional, Arts, E. G. J. M., additional, Wester, N. E., additional, Groen, H., additional, van Echten-Arends, J., additional, and Land, J. A., additional
Published: 2011
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25. POSTER VIEWING SESSION - EARLY PREGNANCY

Author: Kamijo, T., primary, Milart, P., additional, Wojcik, K., additional, Szkodziak, P., additional, Wozniak, S., additional, Czuczwar, P., additional, Paszkowski, T., additional, Landolsi, H., additional, Yacoubi, M. T., additional, Stita, W., additional, Gribaa, M., additional, Hmissa, S., additional, Molenaar, N., additional, van Besouw, N. H., additional, Steegers, E. A. P., additional, Visser, W., additional, de Kuiper, P., additional, de Krijger, R., additional, Exalto, N., additional, Lagrand, R., additional, Kaandorp, S. P., additional, Mellink, C. H. M., additional, van Wely, M., additional, Redeker, E. J. W., additional, Knegt, A. C., additional, Goddijn, M., additional, Vidal, C., additional, Giles, J., additional, Meseguer, M., additional, Zuzuarregui, J. L., additional, Bosch, E., additional, Pellicer, A., additional, Schust, D., additional, Sugimoto, M., additional, Sugimoto, J., additional, Reus, A. D., additional, Stephenson, M. D., additional, Krijger de, R. R., additional, Dunne van, F. M., additional, Exacoustos, C., additional, Vaquero, E., additional, Di Giovanni, A., additional, Romeo, V., additional, Lazzarin, N., additional, Arduini, D., additional, Brahem, S., additional, Mehdi, M., additional, Atig, F., additional, Ghedir, H., additional, Ibala, S., additional, Ajina, M., additional, Saad, A., additional, Chang, C., additional, Wang, H., additional, Huang, S., additional, Pai, S., additional, Soong, Y., additional, Papanikolaou, E., additional, Pantos, G., additional, Grimbizis, G., additional, Bili, E., additional, Polyzos, N., additional, Karastefanou, K., additional, Humaidan, P., additional, Esteves, S., additional, Tarlatzis, B., additional, McNamee, K., additional, Topping, A., additional, Farquharson, R. G., additional, Dawood, F., additional, Ruiz Galdon, M., additional, Lendinez, A. M., additional, Palomares, A. R., additional, Martinez, F., additional, Perez-Nevot, B., additional, Jimenez Fernandez, A., additional, Reyes-Engel, A., additional, Horcajadas, J. A., additional, Savaris, R. F., additional, Kovac, V., additional, Reljic, M., additional, Vlaisavljevic, V., additional, Colicchia, A., additional, Pergolini, I., additional, Gilio, B., additional, Rampini, M. R., additional, Alfano, P., additional, Marconi, D., additional, Verlengia, C., additional, Alviggi, E., additional, Bellver, J., additional, Cruz, F., additional, Martinez, M. C., additional, Ramirez, J., additional, Ferro, J., additional, Garrido, N., additional, Brown, J. K., additional, Lauer, K. B., additional, Inglis, N. F., additional, Critchley, H. O. D., additional, Horne, A. W., additional, Samli, H., additional, Cetinkaya Demir, B., additional, Ozgoz, A., additional, Atalay, M. A., additional, Uncu, G., additional, Yan, Y., additional, Cai-hong, M. A., additional, Jie, Q. I. A. O., additional, Xin-na, C. H. E. N., additional, Weimar, C. H. E., additional, Kavelaars, A., additional, Gellersen, B., additional, Brosens, J. J., additional, de Vreeden-Elbertse, J. M. T., additional, Heijnen, C. J., additional, Macklon, N. S., additional, Castillo, J. C., additional, Dolz, M., additional, Caballero, O., additional, Abad, L., additional, Perez-Panades, J., additional, Bonilla-Musoles, F., additional, Eggert - Kruse, W., additional, Scholz, S., additional, Klopsch, I., additional, and Strowitzki, T., additional
Published: 2011
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26. POSTER VIEWING SESSION - REPRODUCTIVE (EPI) GENETICS

Author: Acar-Perk, B., primary, Weimer, J., additional, Koch, K., additional, Salmassi, A., additional, Arnold, N., additional, Mettler, L., additional, Schmutzler, A. G., additional, Ottolini, C. S., additional, Griffin, D. K., additional, Handyside, A. H., additional, Summers, M. C., additional, Thornhill, A. R., additional, Montjean, D., additional, Benkhalifa, M., additional, Cohen-Bacrie, P., additional, Siffroi, J. P., additional, Mandelbaum, J., additional, Berthaut, I., additional, Bashamboo, A., additional, Ravel, C., additional, McElreavey, K., additional, Ao, A., additional, Zhang, X. Y., additional, Yilmaz, A., additional, Chung, J. T., additional, Demirtas, E., additional, Son, W. Y., additional, Dahan, M., additional, Buckett, W., additional, Holzer, H., additional, Tan, S. L., additional, Perheentupa, A., additional, Vierula, M., additional, Jorgensen, N., additional, Skakkebaek, N. E., additional, Chantot-Bastaraud, S., additional, Toppari, J., additional, Muzii, L., additional, Magli, M. C., additional, Gioia, L., additional, Mattioli, M., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Koscinski, I., additional, Elinati, E., additional, Fossard, C., additional, Kuentz, P., additional, Kilani, Z., additional, Demirol, A., additional, Gurgan, T., additional, Schmitt, F., additional, Velez de la Calle, J., additional, Iqbal, N., additional, Louanjli, N., additional, Pasquier, M., additional, Carre-Pigeon, F., additional, Muller, J., additional, Barratt, C., additional, Viville, S., additional, Magli, C., additional, Grugnetti, C., additional, Castelletti, E., additional, Paviglianiti, B., additional, Pepas, L., additional, Braude, P., additional, Grace, J., additional, Bolton, V., additional, Khalaf, Y., additional, El-Toukhy, T., additional, Galeraud-Denis, I., additional, Bouraima, H., additional, Sibert, L., additional, Rives, N., additional, Carreau, S., additional, Janse, F., additional, de With, L. M., additional, Fauser, B. C. J. M., additional, Lambalk, C. B., additional, Laven, J. S. E., additional, Goverde, A. J., additional, Giltay, J. C., additional, De Leo, V., additional, Governini, L., additional, Quagliariello, A., additional, Margollicci, M. A., additional, Piomboni, P., additional, Luddi, A., additional, Miyamura, H., additional, Nishizawa, H., additional, Ota, S., additional, Suzuki, M., additional, Inagaki, A., additional, Egusa, H., additional, Nishiyama, S., additional, Kato, T., additional, Nakanishi, I., additional, Fujita, T., additional, Imayoshi, Y., additional, Markoff, A., additional, Yanagihara, I., additional, Udagawa, Y., additional, Kurahashi, H., additional, Alvaro Mercadal, B., additional, Imbert, R., additional, Demeestere, I., additional, De Leener, A., additional, Englert, Y., additional, Costagliola, S., additional, Delbaere, A., additional, Velilla, E., additional, Colomar, A., additional, Toro, E., additional, Chamosa, S., additional, Alvarez, J., additional, Lopez-Teijon, M., additional, Fernandez, S., additional, Hosoda, Y., additional, Hasegawa, A., additional, Morimoto, N., additional, Wakimoto, Y., additional, Ito, Y., additional, Komori, S., additional, Sati, L., additional, Zeiss, C., additional, Demir, R., additional, McGrath, J., additional, Ku, S. Y., additional, Kim, Y. J., additional, Kim, Y. Y., additional, Kim, H. J., additional, Park, K. E., additional, Kim, S. H., additional, Choi, Y. M., additional, Moon, S. Y., additional, Minor, A., additional, Chow, V., additional, Ma, S., additional, Martinez Mendez, E., additional, Gaytan, M., additional, Linan, A., additional, Pacheco, A., additional, San Celestino, M., additional, Nogales, C., additional, Ariza, M., additional, Cernuda, D., additional, Bronet, F., additional, Lendinez Ramirez, A. M., additional, Palomares, A. R., additional, Perez-Nevot, B., additional, Urraca, V., additional, Ruiz Martin, A., additional, Reche, A., additional, Ruiz Galdon, M., additional, Reyes-Engel, A., additional, Treff, N. R., additional, Tao, X., additional, Taylor, D., additional, Levy, B., additional, Ferry, K. M., additional, Scott Jr., R. T., additional, Vasan, S., additional, Acharya, K. K., additional, Vasan, B., additional, Yalaburgi, R., additional, Ganesan, K. K., additional, Darshan, S. C., additional, Neelima, C. H., additional, Deepa, P., additional, Akhilesh, B., additional, Sravanthi, D., additional, Sreelakshmi, K. S., additional, Deepti, H., additional, van Doorninck, J. H., additional, Eleveld, C., additional, van der Hoeven, M., additional, Birnie, E., additional, Steegers, E. A. P., additional, Galjaard, R. J., additional, van den Berg, I. M., additional, Fiorentino, F., additional, Spizzichino, L., additional, Bono, S., additional, Biricik, A., additional, Kokkali, G., additional, Rienzi, L., additional, Ubaldi, F. M., additional, Iammarrone, E., additional, Gordon, A., additional, Pantos, K., additional, Oitmaa, E., additional, Tammiste, A., additional, Suvi, S., additional, Punab, M., additional, Remm, M., additional, Metspalu, A., additional, Salumets, A., additional, Rodrigo, L., additional, Mir, P., additional, Cervero, A., additional, Mateu, E., additional, Mercader, A., additional, Vidal, C., additional, Giles, J., additional, Remohi, J., additional, Pellicer, A., additional, Martin, J., additional, Rubio, C., additional, Mozdarani, H., additional, Moghbeli Nejad, S., additional, Behmanesh, M., additional, Alleyasin, A., additional, Ghedir, H., additional, Ibala-Romdhane, S., additional, Mamai, O., additional, Brahem, S., additional, Elghezal, H., additional, Ajina, M., additional, Gribaa, M., additional, Saad, A., additional, Martinez, M. C., additional, Peinado, V., additional, Milan, M., additional, Al-Asmar, N., additional, Buendia, P., additional, Delgado, A., additional, Escrich, L., additional, Amorocho, B., additional, Simon, C., additional, Petrussa, L., additional, Van de Velde, H., additional, De Munck, N., additional, De Rycke, M., additional, Altmae, S., additional, Martinez-Conejero, J. A., additional, Esteban, F. J., additional, Ruiz-Alonso, M., additional, Stavreus-Evers, A., additional, Horcajadas, J. A., additional, Bug, B., additional, Raabe-Meyer, G., additional, Bender, U., additional, Zimmer, J., additional, Schulze, B., additional, Vogt, P. H., additional, Laisk, T., additional, Peters, M., additional, Grabar, V., additional, Feskov, A., additional, Zhilkova, E., additional, Sugawara, N., additional, Maeda, M., additional, Seki, T., additional, Manome, T., additional, Nagai, R., additional, Araki, Y., additional, Georgiou, I., additional, Lazaros, L., additional, Xita, N., additional, Chatzikyriakidou, A., additional, Kaponis, A., additional, Grigoriadis, N., additional, Hatzi, E., additional, Grigoriadis, I., additional, Sofikitis, N., additional, Zikopoulos, K., additional, Gunn, M., additional, Brezina, P. R., additional, Benner, A., additional, Du, L., additional, Kearns, W. G., additional, Shen, X., additional, Zhou, C., additional, Xu, Y., additional, Zhong, Y., additional, Zeng, Y., additional, Zhuang, G., additional, Gunn, M. C., additional, Richter, K., additional, Andreeva, P., additional, Dimitrov, I., additional, Konovalova, M., additional, Kyurkchiev, S., additional, Shterev, A., additional, Daser, A., additional, Day, E., additional, Turley, H., additional, Immesberger, A., additional, Haaf, T., additional, Hahn, T., additional, Dear, P. H., additional, Schorsch, M., additional, Don, J., additional, Golan, N., additional, Eldar, T., additional, and Yaverboim, R., additional
Published: 2011
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27. Unique Transcriptome, Pathways, and Networks in the Human Endometrial Fibroblast Response to Progesterone in Endometriosis

Author: Aghajanova, L., primary, Tatsumi, K., additional, Horcajadas, J. A., additional, Zamah, A. M., additional, Esteban, F. J., additional, Herndon, C. N., additional, Conti, M., additional, and Giudice, L. C., additional
Published: 2010
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28. Posters * Reproductive Endocrinology (i.e. PCOS, Menarche, Menopause etc.)

Author: Fujii, R., primary, Fujita, S., additional, Waseda, T., additional, Oka, Y., additional, Takagi, H., additional, Tomizawa, H., additional, Sasagawa, T., additional, Makinoda, S., additional, Cavagna, M., additional, Braga, D. P. A. F., additional, Figueira, R. C. S., additional, Aoki, T., additional, Maldonado, L. G. L., additional, Iaconelli, A., additional, Borges, E., additional, Prabhakar, s., additional, Dittrich, R., additional, Beckmann, M. W., additional, Hoffmann, I., additional, Mueller, A., additional, Kjotrod, S., additional, Carlsen, S. M., additional, Rasmussen, P. E., additional, Holst-Larsen, T., additional, Mellembakken, J., additional, Thurin-Kjellberg, A., additional, Haapaniemi Kouru, K., additional, Morin Papunen, L., additional, Humaidan, P., additional, Sunde, A., additional, von During, V., additional, Pappalardo, S., additional, Valeri, C., additional, Crescenzi, F., additional, Manna, C., additional, Sallam, H. N., additional, Polec, A., additional, Raki, M., additional, Tanbo, T., additional, Abyholm, T., additional, Fedorcsak, P., additional, Tabanelli, C., additional, Ferraretti, A. P., additional, Feliciani, E., additional, Magli, M. C., additional, Fasolino, C., additional, Gianaroli, L., additional, Wang, T., additional, Feng, C., additional, Song, Y., additional, Dong, M. Y., additional, Sheng, J. Z., additional, Huang, H. F., additional, Sayyah Melli, M., additional, Kazemi-shishvan, M., additional, Snajderova, M., additional, Zemkova, D., additional, Pechova, M., additional, Teslik, L., additional, Lanska, V., additional, Ketel, I., additional, Serne, E., additional, Stehouwer, C., additional, Korsen, T., additional, Hompes, P., additional, Smulders, Y., additional, Voorstemans, L., additional, Homburg, R., additional, Lambalk, C., additional, Bellver, J., additional, Martinez-Conejero, J. A., additional, Pellicer, A., additional, Labarta, E., additional, Alama, P., additional, Melo, M. A. B., additional, Horcajadas, J. A., additional, Agirregoitia, N., additional, Peralta, L., additional, Mendoza, R., additional, Exposito, A., additional, Matorras, R., additional, Agirregoitia, E., additional, Ajina, M., additional, Chaouache, N., additional, Gaddas, M., additional, Souissi, A., additional, Tabka, Z., additional, Saad, A., additional, Zaouali-Ajina, M., additional, Zbidi, A., additional, Eguchi, N., additional, Jinno, M., additional, Watanabe, A., additional, Hirohama, J., additional, Hatakeyama, N., additional, Choi, Y. M., additional, Kim, J. J., additional, Kim, D. H., additional, Yoon, S. H., additional, Ku, S. Y., additional, Kim, S. H., additional, Kim, J. G., additional, Lee, K. S., additional, Moon, S. Y., additional, Xiong, Y., additional, Liang, X., additional, Li, Y., additional, Yang, X., additional, Wei, L., additional, Fujii, R., additional, Utsunomiya, T., additional, Chu, S., additional, Li, P., additional, Akarsu, S., additional, Dirican, E. K., additional, Akin, K. O., additional, Kormaz, C., additional, Goktolga, U., additional, Ceyhan, S. T., additional, Kara, C., additional, Nadamoto, K., additional, Tarui, S., additional, Ida, M., additional, Sugihara, K., additional, Haruki, A., additional, Hukuda, A., additional, Morimoto, Y., additional, Albu, A., additional, Albu, D., additional, Sandu, L., additional, Kong, G., additional, Cheung, L., additional, Lok, I., additional, Pinto, A., additional, Teixeira, L., additional, Figueiredo, H., additional, Pires, I., additional, Silva Carvalho, J. L., additional, Pereira, M. L., additional, Faut, M., additional, de Zuniga, I., additional, Colaci, D., additional, Barrios, E., additional, Oubina, A., additional, Terrado Gil, G., additional, Motta, A., additional, Horton, M., additional, Sobral, F., additional, Gomez Pena, M., additional, Gleicher, N., additional, Barad, D. H., additional, Li, Y. P., additional, Zhao, H. C., additional, Spaczynski, R. Z., additional, Guzik, P., additional, Banaszewska, B., additional, Krauze, T., additional, Wykretowicz, A., additional, Wysocki, H., additional, Pawelczyk, L., additional, Sarikaya, E., additional, Gulerman, C., additional, Cicek, N., additional, Mollamahmutoglu, L., additional, Venetis, C. A., additional, Kolibianakis, E. M., additional, Toulis, K., additional, Goulis, D., additional, Loutradi, K., additional, Chatzimeletiou, K., additional, Papadimas, I., additional, Bontis, I., additional, Tarlatzis, B. C., additional, Schultze-Mosgau, A., additional, Griesinger, G., additional, Schoepper, B., additional, Cordes, T., additional, Diedrich, K., additional, Al-Hasani, S., additional, Gomez, R., additional, Jovanovic, V., additional, Sauer, C. M., additional, Shawber, C. J., additional, Sauer, M. V., additional, Kitajewski, J., additional, Zimmermann, R. C., additional, Bungum, L., additional, Jacobsson, A. K., additional, Rosen, F., additional, Becker, C., additional, Andersen, C. Y., additional, Guner, N., additional, Giwercman, A., additional, Kiapekou, E., additional, Zapanti, E., additional, Boukelatou, D., additional, Mavreli, T., additional, Bletsa, R., additional, Stefanidis, K., additional, Drakakis, P., additional, Mastorakos, G., additional, Loutradis, D., additional, Malhotra, N., additional, Sharma, V., additional, Kumar, S., additional, Roy, K. K., additional, Sharma, J. B., additional, Ferraretti, A., additional, Crippa, A., additional, Stanghellini, I., additional, Robles, F., additional, Serdynska-Szuster, M., additional, Kristensen, S. L., additional, Ernst, E., additional, Toft, G., additional, Olsen, S. F., additional, Bonde, J. P., additional, Vested, A., additional, Ramlau-Hansen, C. H., additional, Wang, F. F., additional, Qu, F., additional, Ding, G. L., additional, Gallot, V., additional, Genro, V., additional, Roux, I., additional, Scheffer, J. B., additional, Frydman, R., additional, Fanchin, R., additional, Kanta Goswami, S., additional, Banerjee, S., additional, Chakravarty, B. N., additional, Kabir, S. N., additional, Seeber, B. E., additional, Morandell, E., additional, Kurzthaler, D., additional, Wildt, L., additional, Dieplinger, H., additional, Tutuncu, L., additional, Bodur, S., additional, Dundar, O., additional, Ron - El, R., additional, Seger, R., additional, Komarovsky, D., additional, Kasterstein, E., additional, Komsky, A., additional, Maslansky, B., additional, Strassburger, D., additional, Ben-Ami, I., additional, Zhao, X. M., additional, Ni, R. M., additional, Lin, L., additional, Dong, M., additional, Tu, C. H., additional, He, Z. H., additional, Yang, D. Z., additional, Karamalegos, C., additional, Polidoropoulos, N., additional, Papanikopoulos, C., additional, Stefanis, P., additional, Argyrou, M., additional, Doriza, S., additional, Sisi, V., additional, Moschopoulou, M., additional, Karagianni, T., additional, Mentorou, C., additional, Economou, K., additional, Davies, S., additional, Mastrominas, M., additional, Gougeon, A., additional, De Los Santos, M. J., additional, Garcia-Laez, V., additional, Esteban, F., additional, Crespo, J., additional, Li, H. W. R., additional, Anderson, R. A., additional, Yeung, W. S. B., additional, Ho, P. C., additional, Ng, E. H. Y., additional, Yang, H. I., additional, Lee, K. E., additional, Seo, S. K., additional, Kim, H. Y., additional, Cho, S. H., additional, Choi, Y. S., additional, Lee, B. S., additional, Park, K. H., additional, Cho, D. J., additional, Hart, R., additional, Doherty, D., additional, Mori, T., additional, Hickey, M., additional, Sloboda, D., additional, Norman, R., additional, Huang, R. C., additional, Beilin, L., additional, Freiesleben, N., additional, Lossl, K., additional, Johannsen, T. H., additional, Loft, A., additional, Bangsboll, S., additional, Hougaard, D., additional, Friis-Hansen, L., additional, Christiansen, M., additional, Nyboe Andersen, A., additional, Thum, M. Y., additional, Abdalla, H., additional, Martinez-Salazar, J., additional, De la Fuente, G., additional, Kohls, G., additional, Garcia Velasco, J. A., additional, Yasmin, E., additional, Kukreja, S., additional, Barth, J., additional, Balen, A. H., additional, Esra, T., additional, Var, T., additional, Citil, A., additional, Dogan, M., additional, Messini, C. I., additional, Dafopoulos, K., additional, Chalvatzas, N., additional, Georgoulias, P., additional, Anifandis, G., additional, Messinis, I. E., additional, Celik, O., additional, Hascalik, S., additional, Celik, N., additional, Sahin, I., additional, Aydin, S., additional, Hanna, C. W., additional, Bretherick, K. L., additional, Liu, C. C., additional, Stephenson, M. D., additional, Robinson, W. P., additional, Louwers, Y. V., additional, Goodarzi, M. O., additional, Taylor, K. D., additional, Jones, M. R., additional, Cui, J., additional, Kwon, S., additional, Chen, Y. D. I., additional, Guo, X., additional, Stolk, L., additional, Uitterlinden, A. G., additional, Laven, J. S. E., additional, Azziz, R., additional, Navaratnarajah, R., additional, Grun, B., additional, Sinclair, J., additional, Dafou, D., additional, Gayther, S., additional, Timms, J. F., additional, Hardiman, P. J., additional, Ye, Y., additional, Wu, R., additional, Ou, J., additional, Kim, S. D., additional, Jee, B. C., additional, Lee, J. Y., additional, Suh, C. S., additional, Jung, J. H., additional, Opmeer, B. C., additional, Broeze, K. A., additional, Coppus, S. F., additional, Collins, J. A., additional, Den Hartog, J. E., additional, Land, J. A., additional, Van der Linden, P. J., additional, Marianowski, P., additional, Ng, E., additional, Van der Steeg, J. W., additional, Steures, P., additional, Strandell, A., additional, Mol, B. W., additional, Tarlatzi, T. B., additional, Kyrou, D., additional, Mertzanidou, A., additional, Fatemi, H. M., additional, Devroey, P., additional, Batenburg, T. E., additional, Konig, T. E., additional, Overbeek, A., additional, Schats, R., additional, Lambalk, C. B., additional, Carone, D., additional, Vizziello, G., additional, Vitti, A., additional, Chiappetta, R., additional, Topcu, H. O., additional, Yuksel, B., additional, Islimye, M., additional, Karakaya, J., additional, ozat, M., additional, Batioglu, S., additional, Kuchenbecker, W. K., additional, Groen, H., additional, Bolster, J. H., additional, van Asselt, S., additional, Wolffenbuettel, B. H., additional, Hoek, A., additional, Wu, Y., additional, Pan, H., additional, Chen, X., additional, Huang, H., additional, Zavos, A., additional, Verikouki, C., additional, Van Os, L., additional, Vink-Ranti, C. Q. J., additional, Rijnders, P. M., additional, Tucker, K. E., additional, Jansen, C. A. M., additional, Lucco, F., additional, Pozzobon, C., additional, Lara, E., additional, Galliano, D., additional, Ballesteros, A., additional, Ghoshdastidar, B., additional, Maity, S. P., additional, Ghoshdastidar, S., additional, Luna, M., additional, Vela, G., additional, Sandler, B., additional, Barritt, J., additional, Flisser, E. D., additional, Copperman, A. B., additional, Nogueira, D., additional, Prat, L., additional, Degoy, J., additional, Bonald, F., additional, Montagut, J., additional, Maity, S., additional, Chen, S., additional, Luo, C., additional, Zhen, H., additional, Shi, X., additional, Wu, F., additional, Ni, Y., additional, Merdassi, G., additional, Chaker, A., additional, Kacem, K., additional, Benmeftah, M., additional, Fourati, S., additional, Wahabi, D., additional, Zhioua, F., additional, Zhioua, A., additional, Saini, P., additional, Saini, A., additional, Sugiyama, R., additional, Nakagawa, K., additional, Nishi, Y., additional, Jyuen, H., additional, Kuribayashi, Y., additional, Inoue, M., additional, Jancar, N., additional, Vrtacnik Bokal, E., additional, Virant-Klun, I., additional, Lee, J. H., additional, Kim, S. G., additional, Cha, E. M., additional, Park, I. H., additional, Lee, K. H., additional, Dahdouh, E. M., additional, Desrosiers, P., additional, St-Michel, P., additional, Villeneuve, M., additional, Fontaine, J. Y., additional, Granger, L., additional, Ramon, O., additional, Burgos, J., additional, Abanto, E., additional, Gonzalez, M., additional, Mugica, J., additional, Corcostegui, B., additional, Tal, J., additional, Ziskind, G., additional, Ohel, G., additional, Paltieli, Y., additional, Paz, G., additional, Lewit, N., additional, Sendel, H., additional, Khouri, S., additional, Calderon, I., additional, van Gelder, P., additional, Al-Inany, H. G., additional, Antaki, R., additional, Dean, N., additional, Lapensee, L., additional, Racicot, M., additional, Menard, S., additional, Kadoch, I., additional, Meylaerts, L. J., additional, Dreesen, L., additional, Vandersteen, M., additional, Neumann, C., additional, Zollner, U., additional, Kato, K., additional, Segawa, T., additional, Kawachiya, S., additional, Okuno, T., additional, Kobayashi, T., additional, Takehara, Y., additional, Kato, O., additional, Jayaprakasan, K., additional, Nardo, L., additional, Hopkisson, J., additional, Campbell, B., additional, and Raine-Fenning, N., additional
Published: 2010
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29. Posters * Endometriosis, Endometrium and Implantation

Author: Jiang, Y., primary, Zhao, J., additional, Hua, M., additional, Zhen, X., additional, Yan, G., additional, Hu, Y., additional, Sun, H., additional, Selvaggi, L., additional, Zannoni, G. F., additional, Tagliaferri, V., additional, De Cicco, S., additional, Vellone, V. G., additional, Romualdi, D., additional, Lanzone, A., additional, Guido, M., additional, Fassbender, A., additional, Vodolazkaia, A. V., additional, Bossuyt, X. B., additional, Kyama, M. K., additional, Meuleman, C. M., additional, Peeraer, K. P., additional, Tomassetti, C. T., additional, D'Hooghe, T. M., additional, Lumini, A., additional, Nanni, L., additional, Manna, C., additional, Pappalardo, S., additional, Melin, A., additional, Lundholm, C., additional, Malki, N., additional, Swahn, M. L., additional, Sparen, P., additional, Bergqvist, A., additional, Crescenzi, F., additional, Farrag, A., additional, Sallam, H. N., additional, Zou, L., additional, Ding, G., additional, Zhang, R., additional, Sheng, J., additional, Huang, H., additional, von Kleinsorgen, C., additional, Wilson, T., additional, Thiel-Moder, U., additional, Ebert, A. D., additional, Reinfandt, M., additional, Papadopolous, T., additional, Melo, A. S., additional, Rodrigues, J. K., additional, Dib, L. A., additional, Andrade, A. Z., additional, Donabela, F. C., additional, Ferriani, R. A., additional, Navarro, P. A., additional, Tocci, A., additional, Royo, P., additional, Lucchini, C., additional, Ramos, P., additional, Alcazar, J. L., additional, Habara, T., additional, Terada, S., additional, Yoshioka, N., additional, Hayashi, N., additional, Haouzi, D., additional, Assou, S., additional, Monzo, C., additional, Anahory, T., additional, Dechaud, H., additional, De Vos, J., additional, Hamamah, S., additional, Gonzalez-Ramos, R., additional, Rojas, C., additional, Rocco, J., additional, Poch, A., additional, Sovino, H., additional, Kohen, P., additional, Munoz, A., additional, Devoto, L., additional, Aygen, M. A., additional, Atakul, T., additional, Oner, G., additional, Ozgun, M. T., additional, Sahin, Y., additional, Ozturk, F., additional, Li, R., additional, Qiao, J., additional, Zhylkova, I., additional, Feskov, A., additional, Feskova, I., additional, Somova, O., additional, Chumakova, N., additional, Bontekoe, S., additional, Blake, D., additional, Heineman, M. J., additional, Williams, E. C., additional, Johnson, N. P., additional, Motta, A., additional, Colaci, D., additional, Horton, M., additional, Faut, M., additional, Bisioli, C., additional, Kopcow, L., additional, de Zuniga, I., additional, Wiener-Megnazi, Z., additional, Khaytov, M., additional, Lahav - Baratz, S., additional, Shiloh, H., additional, Koifman, M., additional, Oslander, R., additional, Dirnfeld, M., additional, Sundqvist, J., additional, Andersson, K. L., additional, Scarselli, G., additional, Gemzell-Danielsson, K., additional, Lalitkumar, P. G. L., additional, Tokushige, N., additional, Markham, R., additional, Crossett, B., additional, Ahn, S., additional, Nelaturi, V., additional, Khan, A., additional, Fraser, I. S., additional, Van Vaerenbergh, I., additional, Fatemi, H. M., additional, Blockeel, C., additional, Van Lommel, L., additional, In't Veld, P., additional, Schuit, F., additional, Kolibianakis, E. M., additional, Devroey, P., additional, Bourgain, C., additional, Sugino, N., additional, Tamura, I., additional, Lee, R., additional, Maekawa, R., additional, Gelbaya, T., additional, Gordts, S., additional, D'Hooghe, T. N., additional, Gergolet, M., additional, Nardo, L. G., additional, Yu, H., additional, Wang, H., additional, Lee, C., additional, Soong, Y., additional, Kremenska, Y., additional, Masliy, Y., additional, Goncharova, Y., additional, Kremenskoy, M., additional, Veselovskyy, V., additional, Zukin, V., additional, Sudoma, I., additional, Delgado-Rosas, F., additional, Gomez, R., additional, Tamarit, S., additional, Abad, A., additional, Simon, C., additional, Pellicer, A., additional, Racicot, M., additional, Dean, N. L., additional, Antaki, R., additional, Menard, S., additional, Kadoch, I. J., additional, Garcia-Guzman, R., additional, Cabrera Romero, L., additional, Hernandez, J., additional, Palumbo, A., additional, Marshall, E., additional, Lowry, J., additional, Maybin, J. A., additional, Collins, F., additional, Critchley, H. O. D., additional, Saunders, P. T. K., additional, Chaudhury, K., additional, Jana, S. K., additional, Banerjee, P., additional, Mukherjee, S., additional, Chakravarty, B. N., additional, Allegra, A., additional, Marino, A., additional, Lama, A., additional, Santoro, A., additional, Agueli, C., additional, Mazzola, S., additional, Volpes, A., additional, Delvoux, B., additional, de Graaff, A. A., additional, Kyama, C. M., additional, Dunselman, G. A. J., additional, Romano, A., additional, Caccavo, D., additional, Pellegrino, N. M., additional, Totaro, I., additional, Panzarino, M., additional, Nardelli, C., additional, Depalo, R., additional, Flores, R., additional, Montanana, V., additional, Monzo, A., additional, Polo, P., additional, Garcia-Gimeno, T., additional, Cabo, A., additional, Rubio, J. M., additional, Beets, G. L., additional, van Lankveld, J. J., additional, Kim, H. Y., additional, Lee, B. S., additional, Cho, S. H., additional, Choi, Y. S., additional, Seo, S. K., additional, Lee, K. E., additional, Yang, H. I., additional, Abubakirov, A., additional, Vacheyshvili, T., additional, Krechetova, L., additional, Ziganshina, M., additional, Demura, T., additional, Nazarenko, T., additional, Fulop, I., additional, Rucz, A., additional, Herczegh, S. Z., additional, Ujvari, A., additional, Takacs, S. Z., additional, Szakonyi, T., additional, Lopez - Muniz, A., additional, Zamora, L., additional, Serra, O., additional, Guix, C., additional, Lopez-Teijon, M., additional, Benadiva, C., additional, Alvarez, J. G., additional, Goudakou, M., additional, Karkanaki, A., additional, Kalogeraki, A., additional, Mataliotakis, I., additional, Kalogiannidis, I., additional, Prapas, I., additional, Hosie, M., additional, Thomson, K. J., additional, Penny, C. B., additional, Penny, C., additional, Hosie, M. J., additional, McKinnon, B., additional, Klaeser, B., additional, Bersinger, N., additional, Mueller, M. D., additional, Horcajadas, J. A., additional, Martinez-Conejero, J. A., additional, Montesinos, M., additional, Morgan, M., additional, Fortuno, S., additional, Yi, K. W., additional, Shin, J. H., additional, Park, H. T., additional, Kim, T., additional, Kim, S. H., additional, Hur, J. Y., additional, Chan, R. W. S., additional, Chan, Y. Y., additional, Ng, E. H. Y., additional, Yeung, W. S. B., additional, Santulli, P., additional, Borghese, B., additional, Chopin, N., additional, Marcellin, L., additional, de Ziegler, D., additional, Chapron, C., additional, Elnashar, A., additional, Badawy, A., additional, Mosbah, A., additional, Tzioras, S., additional, Polyzos, N. P., additional, Messini, C. I., additional, Papanikolaou, E. G., additional, Valachis, A., additional, Patavoukas, E., additional, Mauri, D., additional, Messinis, I. E., additional, Acar, N., additional, Hirota, Y., additional, Tranguch, S., additional, Daikoku, T., additional, Burnum, K. E., additional, Xie, H., additional, Kodama, A., additional, Osuga, Y., additional, Ustunel, I., additional, Friedman, D. B., additional, Caprioli, R. M., additional, Dey, S. K., additional, Mitra, A., additional, Sahu, R., additional, Pal, M., additional, Bhattachrayya, A. K., additional, Bhattachrya, J., additional, Ferrero, S., additional, Remorgida, V., additional, Rollandi, G. A., additional, Biscaldi, E., additional, Cho, S., additional, Arena, E., additional, Morando, A., additional, Tomazevic, T., additional, Ban-Frangez, H., additional, Virant-Klun, I., additional, Verdenik, I., additional, Pozlep, B., additional, Vrtacnik-Bokal, E., additional, Valenzano Menada, M., additional, Morotti, M., additional, Venturini, P. L., additional, Dimitriadis, E., additional, Salamonsen, L. A., additional, Hannan, N., additional, O'Connor, O., additional, Rombauts, L., additional, Stoikos, C., additional, Mahmoudi, M., additional, Shaikh, A., additional, Mousavifar, N., additional, Rastin, M., additional, Baharara, J., additional, Tabasi, N., additional, Takemura, Y., additional, Fujimoto, A., additional, Tsutsumi, R., additional, Ooi, N., additional, Yano, T., additional, Taketani, Y., additional, Panagiotidis, I., additional, Prapas, Y., additional, Zhang, D., additional, Lv, P. P., additional, Ding, G. L., additional, Zhang, R. J., additional, Zou, L. B., additional, Xu, G. F., additional, Gao, H. J., additional, Zhu, Y. M., additional, Sheng, J. Z., additional, Huang, H. F., additional, Labarta, E., additional, Alama, P., additional, and Bosch, E., additional
Published: 2010
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30. 529. PROPROTEIN CONVERTASE 6 PLAYS A CRITICAL ROLE IN MODULATING THE HUMAN ENDOMETRIAL EPITHELIUM FOR RECEPTIVITY AND IMPLANTATION

Author: Nie, G., primary, Li, Y., additional, Salamonsen, L. A., additional, Simon, C., additional, Quiñonero, A., additional, Horcajadas, J., additional, Rombauts, L., additional, and Heng, S., additional
Published: 2009
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31. Comparative protein-profile analysis of implanted versus non-implanted human blastocysts

Author: Dominguez, F., primary, Gadea, B., additional, Esteban, F. J., additional, Horcajadas, J. A., additional, Pellicer, A., additional, and Simon, C., additional
Published: 2008
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32. Implantation of the Human Embryo: Research Lines and Models.

Author: Bischof, P., Aplin, J. D., Bentin-Ley, U., Brannstrom, M., Casslen, B., Castrillo, J. L., Classen-Linke, I., Critchley, H. O. D., Devoto, L., D'Hooghe, T., Horcajadas, J. A., Groothuis, P., Ivell, R., Pongrantz, I., Macklon, N. S., Sharkey, A., Vicovac, L., White, J. O., Winterhager, E., and Von Wolff, M.
Subjects: HUMAN embryo transfer, ENDOMETRIUM, FEMALE infertility, HUMAN fertility, WOMEN'S health, REPRODUCTIVE technology, ABORTION
Abstract: Infertility is an increasing problem all over the world, and it has been estimated that 10–15% of couples in fertile age have fertility problems. Likewise induced unsafe abortion is a serious threat to women’s health. Despite advances made in assisted reproduction techniques, little progress has been made in increasing the success rate during fertility treatment. This document describes a wide range of projects carried out to increase the understanding in the field of embryo implantation research. The ‘Fruitful’ research network was created to encourage collaborations within the consortium and to describe our different research potentials to granting agencies or private sponsors. Copyright © 2006 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
Published: 2006
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33. Leptin system in embryo development and implantation: a protein in search of a function.

Author: Cervero, A., Horcajadas, J. A., Dominguez, F., Pellicer, A., and Simón, C.
Subjects: *HUMAN embryos, *TRANSPLANTATION of organs, tissues, etc., *LEPTIN, *ENDOMETRIUM, *BLASTOCYST
Abstract: Implantation is a crucial moment in the reproduction process that requires perfect synchronization between the embryo and the material endometrium. The embryo must reach the blastocyst stage and the endometrium must be prepared to receive it. An appropriate and specific molecular dialogue must also take place between them. There is ample evidence to show that the leptin system is implicated in this cross-talk. Examples are described. Although there is some controversy surrounding the data, they are supported by the presence of leplin receptor mRNA in mouse and human oocytes and embryos throughout preimplantation development. Otherwise, the leptin mRNA is only detected at the blastocyst stage in both human and mouse. Furthermore, leptin is found at higher concentrations in the conditioned media from competent human blastocysts than in those from arrested embryos, suggesting that this molecule is a marker for blastocyst viability. Given that expression of the leptin receptor increases in the human endometrium during the luteal phase, the secreted leptin could trigger its activation. Finally, leptin and the leptin receptor have been detected in implantation sites. All these findings point to the involvement of the leptin system in the molecular mechanism of the implantation process and embryo development. [ABSTRACT FROM AUTHOR]
Published: 2005
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34. Effects of ovarian stimulation on endometrial gene expression profile.

Author: Pellicer, A., Conejero, J. A., Horcajadas, J. A., and Simón, C.
Subjects: *OVARIES, *REPRODUCTIVE technology, *EMBRYO transfer, *MENSTRUAL cycle, *OVUM, *RNA
Abstract: Controlled ovarian stimulation (COS) used in assisted reproduction techniques (ART) produces lower implantation rates per embryo transferred as compared to natural and ovum donation cycles, suggesting a suboptimal endometrial development. Endometrial alterations have been observed by histological and biochemical techniques during decades. The recent developments in functional genomics have provided objective tools to analyze the endometrium in natural cycles and evaluate the impact of COS protocols in endometrial development. COS cycles that use GnRH agonists and antagonists have been analyzed in detail during the window of implantation to establish the differences with respecting to the natural cycle [1-5]. Even more, it has been demonstrated that endometria from natural cycles follow different genomic patterns compared to COS cycles in the transition from the pre-receptive (days LH/hCG+1 until LH/hCG+5) to the receptive phase (day LH+7/hCG+7). Specifically, it has been demonstrated the existence of a two-day delay in the activation/repression of two clusters composed by 218 and 133 genes on day hCG+7 versus LH+7 [6]. To further increase our understanding of the pathways governing endometrial receptivity, we have expanded these studies by comparing the gene expression profile of the human endometrium troughout the early-mid secretory phase in natural cycles vs controlled ovarian stimulated (COS) cycles. We have analyzed endometrial samples collected from healthy fertile cycling ovum donors (aged 23-39), that underwent either a natural cycle (n = 25) or stimulated cycles (n = 25) at days LH+1, LH+3, LH+5, LH +7 and LH+9 (n = 5 per time point), no progesterone (P) supplementation was administered. RNA was extracted and labeled cDNA were hybridized onto the GeneChip HG&lowbar;U133A (Affymetrix) for the comparisons. Gene expression data were analysed using different methods such as clustering or selection of differentially expressed genes, as implemented in the GEPAS (http://www.gepas.org). We analyzed their corresponding temporal functional profiles. For that end, we used the first day as reference and we compared each subsequent day to this reference time by a gene set enrichment analysis, as implemented in the FatiScan tool of Babelomics. This method allows us to trace the functional blocks (GO, KEGG pathways, etc.) that were significantly up- and down-regulated on each day of the WOI. Our results demonstrate that the receptivity transition from pre-receptive (LH+1, LH+3 and LH+5) to receptive (LH+7, LH+9) endometria displayed a differential profile of biological processes over and under expressed, pathways statistically over and under represented and differential gene clustering. Furthermore, these results indicate that gene expression profiling of the endometrium at the time of implantation is different between natural cycles specifically at LH+7 and COH cycles at hCG+7 and similarities are recovered thereafter. Based on this basic data, influences of endometrial function on IVF outcomes will be reviewed as well state of the art of human endometrial receptivity markers. [ABSTRACT FROM AUTHOR]
Published: 2010
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35. Endometrial receptivity is affected in women with high circulating progesterone levels at the end of the follicular phase: a functional genomics analysis.

Author: Labarta E, Martínez-Conejero JA, Alamá P, Horcajadas JA, Pellicer A, Simón C, and Bosch E
Subjects: Adult, Endometrium metabolism, Endometrium pathology, Female, Follicular Phase genetics, Gene Expression Profiling, Gene Expression Regulation, Genomics, Humans, Oligonucleotide Array Sequence Analysis, Ovulation Induction, Pregnancy, Prospective Studies, Embryo Implantation genetics, Endometrium physiology, Follicular Phase metabolism, Progesterone blood
Abstract: Background: Elevated serum progesterone levels at the end of the follicular phase in controlled ovarian stimulation (COS) leads to a poorer ongoing pregnancy rate in IVF cycles due to reduced endometrial receptivity. The objective of this study was to use microarray technology to compare endometrial gene expression profiles at the window of implantation according to the levels of circulating progesterone., Methods: For this prospective cohort study, microarray data were obtained from endometrial biopsies from 12 young healthy oocyte donors undergoing COS with pituitary suppression by either gonadotrophin-releasing hormone (GnRH) agonists or antagonists, and recombinant FSH. On the day of recombinant chorionic gonadotrophin (rCG) administration, six women had serum progesterone levels (P) >1.5 ng/ml (study group) and six had serum P levels <1.5 ng/ml (control group). Endometrial samples were collected using a Pipelle catheter 7 days after the rCG injection., Results: Using the parametric test, we identified 140 genes significantly dysregulated (64 up- and 76 down-regulated) in the study group endometria compared with the control endometria, regardless of the GnRH analogue employed. These genes are related to cell adhesion, developmental processes, the immune system and others, which are all required for normal endometrial function development. Of the 25 gene targets previously proposed as markers for endometrial receptivity, 13 appeared over-regulated in the study group., Conclusions: Our results reveal that elevated progesterone levels on the day of rCG administration can induce significant alterations in the gene expression profile of the endometrium.
Published: 2011
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36. Unique transcriptome, pathways, and networks in the human endometrial fibroblast response to progesterone in endometriosis.

Author: Aghajanova L, Tatsumi K, Horcajadas JA, Zamah AM, Esteban FJ, Herndon CN, Conti M, and Giudice LC
Subjects: Adult, Base Sequence, Case-Control Studies, DNA Primers genetics, Drug Resistance genetics, Female, Fibroblasts drug effects, Fibroblasts metabolism, Gene Expression drug effects, Gene Expression Profiling, Gene Regulatory Networks drug effects, Humans, In Vitro Techniques, Middle Aged, Peritoneal Diseases genetics, Peritoneal Diseases metabolism, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction drug effects, Endometriosis genetics, Endometriosis metabolism, Endometrium drug effects, Endometrium metabolism, Progesterone pharmacology
Abstract: Eutopic endometrium in endometriosis has molecular evidence of resistance to progesterone (P(4)) and activation of the PKA pathway in the stromal compartment. To investigate global and temporal responses of eutopic endometrium to P(4), we compared early (6-h), intermediate (48-h), and late (14-Day) transcriptomes, signaling pathways, and networks of human endometrial stromal fibroblasts (hESF) from women with endometriosis (hESF(endo)) with hESF from women without endometriosis (hESF(nonendo)). Endometrial biopsy samples were obtained from subjects with and without mild peritoneal endometriosis (n = 4 per group), and hESF were isolated and treated with P(4) (1 μM) plus estradiol (E(2)) (10 nM), E(2) alone (10 nM), or vehicle for up to 14 days. Total RNA was subjected to microarray analysis using a Gene 1.0 ST (Affymetrix) platform and analyzed by using bioinformatic algorithms, and data were validated by quantitative real-time PCR and ELISA. Results revealed unique kinetic expression of specific genes and unique pathways, distinct biological and molecular processes, and signaling pathways and networks during the early, intermediate, and late responses to P(4) in both hESF(nonendo) and hESF(endo), although a blunted response to P(4) was observed in the latter. The normal response of hESF to P(4) involves a tightly regulated kinetic cascade involving key components in the P(4) receptor and MAPK signaling pathways that results in inhibition of E(2)-mediated proliferation and eventual differentiation to the decidual phenotype, but this was not established in the hESF(endo) early response to P(4). The abnormal response of this cell type to P(4) may contribute to compromised embryonic implantation and infertility in women with endometriosis.
Published: 2011
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37. Effect of ICSI on gene expression and development of mouse preimplantation embryos.

Author: Giritharan G, Li MW, Di Sebastiano F, Esteban FJ, Horcajadas JA, Lloyd KC, Donjacour A, Maltepe E, and Rinaudo PF
Subjects: Animals, DNA-Binding Proteins biosynthesis, Embryo Culture Techniques, Embryonic Development, Female, Histone Deacetylase 6, Histone Deacetylases biosynthesis, Histone-Lysine N-Methyltransferase, Mice, Myeloid-Lymphoid Leukemia Protein biosynthesis, Protein Array Analysis, Transcription Factors biosynthesis, Blastocyst metabolism, Gene Expression Profiling, Gene Expression Regulation, Developmental, Sperm Injections, Intracytoplasmic
Abstract: Background: In vitro culture (IVC) and IVF of preimplantation mouse embryos are associated with changes in gene expression. It is however not known whether ICSI has additional effects on the transcriptome of mouse blastocysts., Methods: We compared gene expression and development of mouse blastocysts produced by ICSI and cultured in Whitten's medium (ICSI(WM)) or KSOM medium with amino acids (ICSI(KSOMaa)) with control blastocysts flushed out of the uterus on post coital Day 3.5 (in vivo). In addition, we compared gene expression in embryos generated by IVF or ICSI using WM. Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip., Results: Blastocysts from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared with embryos generated in vivo. Approximately 1000 genes are differentially expressed between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after IVC. Unexpectedly, expression of only 41 genes was significantly different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOM(aa))., Conclusions: Our results suggest that fertilization by ICSI may play a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.
Published: 2010
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38. Endometrial gene expression analysis at the time of embryo implantation in women with unexplained infertility.

Author: Altmäe S, Martínez-Conejero JA, Salumets A, Simón C, Horcajadas JA, and Stavreus-Evers A
Subjects: Adult, Embryo Implantation genetics, Female, Gene Expression Profiling, Humans, Oligonucleotide Array Sequence Analysis, Pregnancy, Principal Component Analysis, Reverse Transcriptase Polymerase Chain Reaction, Embryo Implantation physiology, Endometrium metabolism, Gene Expression Regulation, Infertility, Female genetics
Abstract: Successful embryo implantation depends on the quality of the embryo, as well as on the receptivity of the endometrium. The aim of this study was to investigate the endometrial gene expression profile in women with unexplained infertility in comparison with fertile controls at the time of embryo implantation in order to find potential predictive markers of uterine receptivity and to identify the molecular mechanisms of infertility. High-density oligonucleotide gene arrays, comprising 44 000 gene targets, were used to define the endometrial gene expression profile in infertile (n = 4) and fertile (n = 5) women during the mid-secretory phase (day LH + 7). Microarray results were validated using real-time PCR. Analyses of expression data were carried out using non-parametric methods. Hierarchical clustering and principal component analysis showed a clear distinction in endometrial gene expression between infertile and fertile women. In total we identified 145 significantly (>3-fold change) up-regulated and 115 down-regulated genes in infertile women versus controls. Via Database for Annotation, Visualization and Integrated Discovery functional analysis we detected a substantial number of dysregulated genes in the endometria of infertile women, involved in cellular localization (21.1%) and transport (18.8%) and transporter activity (13.1%) and with major localization in extracellular regions (19.2%). Ingenuity Pathways Analysis of the gene list showed dysregulation of gene pathways involved in leukocyte extravasation signalling, lipid metabolism and detoxification in the endometria of infertile women. In conclusion, endometrial gene expression in women with unexplained infertility at the time of embryo implantation is markedly different from that in fertile women. These results provide new information on genes and pathways that may have functional significance as regards to endometrial receptivity and subsequent embryo implantation.
Published: 2010
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39. Predicting adverse obstetric outcome after early pregnancy events and complications: a review.

Author: van Oppenraaij RH, Jauniaux E, Christiansen OB, Horcajadas JA, Farquharson RG, and Exalto N
Subjects: Abortion, Induced adverse effects, Birth Weight, Crown-Rump Length, Female, Humans, Pregnancy, Pregnancy Trimester, First, Prognosis, Risk Assessment, Pregnancy Complications diagnosis, Pregnancy Outcome
Abstract: BACKGROUND The aim was to evaluate the impact of early pregnancy events and complications as predictors of adverse obstetric outcome. METHODS We conducted a literature review on the impact of first trimester complications in previous and index pregnancies using Medline and Cochrane databases covering the period 1980-2008. RESULTS Clinically relevant associations of adverse outcome in the subsequent pregnancy with an odds ratio (OR) > 2.0 after complications in a previous pregnancy are the risk of perinatal death after a single previous miscarriage, the risk of very preterm delivery (VPTD) after two or more miscarriages, the risk of placenta praevia, premature preterm rupture of membranes, VPTD and low birthweight (LBW) after recurrent miscarriage and the risk of VPTD after two or more termination of pregnancy. Clinically relevant associations of adverse obstetric outcome in the ongoing pregnancy with an OR > 2.0 after complications in the index pregnancy are the risk of LBW and very low birthweight (VLBW) after a threatened miscarriage, the risk of pregnancy-induced hypertension, pre-eclampsia, placental abruption, preterm delivery (PTD), small for gestational age and low 5-min Apgar score after detection of an intrauterine haematoma, the risk of VPTD and intrauterine growth restriction after a crown-rump length discrepancy, the risk of VPTD, LBW and VLBW after a vanishing twin phenomenon and the risk of PTD, LBW and low 5-min Apgar score in a pregnancy complicated by severe hyperemesis gravidarum. CONCLUSIONS Data from our literature review indicate, by finding significant associations, that specific early pregnancy events and complications are predictors for subsequent adverse obstetric and perinatal outcome. Though, some of these associations are based on limited or small uncontrolled studies. Larger population-based controlled studies are needed to confirm these findings. Nevertheless, identification of these risks will improve obstetric care.
Published: 2009
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40. Microarray analysis in sperm from fertile and infertile men without basic sperm analysis abnormalities reveals a significantly different transcriptome.

Author: Garrido N, Martínez-Conejero JA, Jauregui J, Horcajadas JA, Simón C, Remohí J, and Meseguer M
Subjects: Antigens, Neoplasm metabolism, Apoptosis Regulatory Proteins metabolism, DNA genetics, Humans, Male, RNA, Messenger metabolism, Semen cytology, Semen metabolism, Spermatozoa abnormalities, Trypsin, Trypsinogen metabolism, gamma-Glutamyltransferase metabolism, Fertility genetics, Gene Expression Profiling, Infertility, Male genetics, Oligonucleotide Array Sequence Analysis, Spermatozoa metabolism
Abstract: Sperm analysis following World Health Organization guidelines is unable to explain the molecular causes of male infertility when basic sperm parameters are within a normal range and women do not present gynecologic pathology. Consequently, there is a need for accurate diagnostic tools in this area, and microarray technology emerges as promising. We present, for the first time, preliminary results of a comparison of sperm mRNA expression profiles between fertile and infertile men with normal semen parameters, discovering profound discrepancies between groups, with potential diagnostic and therapeutic possibilities.
Published: 2009
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41. CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines.

Author: Dominguez F, Martínez S, Quiñonero A, Loro F, Horcajadas JA, Pellicer A, and Simón C
Subjects: Adult, Blastocyst cytology, Blastocyst drug effects, Blastocyst metabolism, Cell Line, Cell Movement drug effects, Chemokine CXCL10 genetics, Chemokine CXCL10 metabolism, Culture Media, Conditioned pharmacology, Endometrium cytology, Endometrium drug effects, Endometrium metabolism, Female, Humans, Immunohistochemistry, Menstrual Cycle metabolism, Protein Array Analysis, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, CXCR3 genetics, Receptors, CXCR3 metabolism, Reverse Transcriptase Polymerase Chain Reaction, Trophoblasts cytology, Trophoblasts metabolism, Chemokine CXCL10 pharmacology, Chemotaxis drug effects, Interleukin-6 pharmacology, Trophoblasts drug effects
Abstract: The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-gamma-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model.
Published: 2008
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42. Identification, characterization and co-localization of label-retaining cell population in mouse endometrium with typical undifferentiated markers.

Author: Cervelló I, Martínez-Conejero JA, Horcajadas JA, Pellicer A, and Simón C
Subjects: Animals, Biomarkers analysis, Bromodeoxyuridine, Cell Differentiation, Female, Immunohistochemistry, Mice, Mice, Inbred ICR, Octamer Transcription Factor-3 metabolism, Proto-Oncogene Proteins c-kit metabolism, Staining and Labeling, Endometrium cytology, Endometrium metabolism, Stem Cells cytology
Abstract: Background: The endometrium, lining of the uterus, is a highly active organ that is remodelled periodically during the lifespan. Different studies suggest the presence of an adult or progenitor stem cell (PSC) population in this tissue because of its cyclic regenerative capacity., Methods: In this study, we aim at identifying and localizing the putative PSC population in the murine uterus using the 5-bromo-2'-deoxyuridine (BrdU) labelling method to detect label-retaining cells (LRCs) that cycle slowly. Uteri from BrdU-treated mice were analysed via single and double immunohistochemistry to co-localize them with the markers of undifferentiation already described such as c-KIT and POU5F1 (also known as OCT-4). Finally, we confirmed the presence of the indicated markers at mRNA level., Results: We observed the presence and gradual decrease of LRCs in the endometrium during the lifespan of the mice. In adulthood, the LRC population decreased notably and remained in the lower region of the stroma in the murine endometrium. Some of the endometrial LRCs co-localized with c-KIT and POU5F1. PCR and nested-PCR confirmed the presence of these undifferentiated markers., Conclusions: We demonstrated that the murine endometrium possesses LRCs with the features of a putative PSC population localized at the lower region of the stroma.
Published: 2007
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43. Wide genomic analysis of human endometrial receptivity: new times, new opportunities.

Author: Horcajadas JA, Pellicer A, and Simón C
Subjects: Animals, Embryo Implantation genetics, Embryo Implantation physiology, Female, Gene Expression Profiling methods, Humans, Menstrual Cycle genetics, Oligonucleotide Array Sequence Analysis, Endometrium physiology, Menstrual Cycle physiology
Abstract: Microarray technology has broadened the insight into many research fields allowing scientists to analyse the expression of many genes in quick and efficient experiments aimed at translating these findings into clinical applications. In reproductive medicine, researchers have exploited microarrays to increase understanding of the molecular mechanisms involved in endometrial receptivity and how a possible therapeutic translation can be feasible. In the last 4 years, several studies have focused on the genomics of the human endometrium in different physiological and pathological conditions, and these studies have generated a large amount of information about the regulation and dysregulation of the window of implantation (WOI) genes in fertile, subfertile and refractory conditions. However, the key molecules/mechanisms in endometrial receptivity remain to be elucidated. In this comprehensive review, we have analysed the available results obtained in our own and other laboratories, defining the genomic profile of the receptive endometrium in different situations and its possible clinical application.
Published: 2007
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44. Similar endometrial development in oocyte donors treated with either high- or standard-dose GnRH antagonist compared to treatment with a GnRH agonist or in natural cycles.

Author: Simon C, Oberyé J, Bellver J, Vidal C, Bosch E, Horcajadas JA, Murphy C, Adams S, Riesewijk A, Mannaerts B, and Pellicer A
Subjects: Adolescent, Adult, Buserelin pharmacology, Chorionic Gonadotropin, Endometrium drug effects, Endometrium pathology, Female, Fertilization in Vitro, Follicle Stimulating Hormone therapeutic use, Gene Expression Regulation, Gonadotropin-Releasing Hormone analogs & derivatives, Gonadotropin-Releasing Hormone pharmacology, Humans, Luteal Phase, Microscopy, Electron, Scanning, Oligonucleotide Array Sequence Analysis, Oocytes metabolism, Oocytes pathology, Ovulation Induction, RNA chemistry, Receptors, Estrogen metabolism, Receptors, Progesterone metabolism, Time Factors, Ultrasonics, Endometrium cytology, Gonadotropin-Releasing Hormone agonists, Gonadotropin-Releasing Hormone antagonists & inhibitors, Oocyte Donation methods, Oocytes cytology
Abstract: Background: This descriptive study evaluates the impact on endometrial development of standard and high doses of a GnRH antagonist in stimulated cycles compared with GnRH agonist and natural cycles., Methods: Thirty-one oocyte donors were treated with a combination of rFSH and 0.25 mg/day ganirelix (standard dose), 2 mg/day ganirelix (high dose) or 0.6 mg/day buserelin (long protocol). Vaginal progesterone (200 mg/day) was administered in the luteal phase. Endometrial biopsies were performed 2 and 7 days after HCG administration. Additional biopsies were carried out in a subset of 12 subjects, 2 and 7 days following the LH peak of their previous natural cycle. Biopsies were evaluated histologically and by scanning electron microscopy. Gene expression profiles were also studied., Results: At HCG +2, all the parameters studied were similar in all the groups and comparable to those observed in the natural cycle. At HCG +7, endometrial dating, steroid receptors and the presence of pinopodes were comparable in both GnRH antagonist groups and in the natural cycle. In buserelin group, endometrial dating and pinopode expression suggested an arrested endometrial development. For window of implantation genes, expression patterns were closer to those in the natural cycle following standard- or high-dose ganirelix than after buserelin administration., Conclusion: No relevant alteration was observed in the endometrial development in the early and mid-luteal phases in women undergoing controlled ovarian stimulation for oocyte donation following daily treatment with a standard- or high-dose GnRH antagonist. In addition, the endometrial development after GnRH antagonist mimics the natural endometrium more closely than after GnRH agonist.
Published: 2005
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45. Analysis of early promoters of the Bacillus bacteriophage GA-1.

Author: Horcajadas JA, Meijer WJ, Rojo F, and Salas M
Subjects: Base Sequence, DNA Replication, Molecular Sequence Data, Bacillus Phages genetics, Promoter Regions, Genetic
Abstract: Bacteriophage GA-1, which infects Bacillus sp. strain G1R, is evolutionarily related to phage phi29, which infects Bacillus subtilis. We report the characterization of several GA-1 promoters located at either end of its linear genome. Some of them are unique for GA-1 and drive the expression of open reading frames that have no counterparts in the genome of phi29 or related phages. These unique promoters are active at early infection times and are repressed at late times. In vitro transcription reactions revealed that the purified GA-1-encoded protein p6 represses the activity of these promoters, although the amount of p6 required to repress transcription was different for each promoter. The level of protein p6 produced in vivo increases rapidly during the first stage of the infection cycle. The protein p6 concentration may serve to modulate the expression of these early promoters as infection proceeds.
Published: 2001
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46. Phi29 family of phages.

Author: Meijer WJ, Horcajadas JA, and Salas M
Subjects: Amino Acid Sequence, Bacillus metabolism, Bacillus Phages chemistry, Bacillus Phages growth & development, Base Sequence, DNA Replication, DNA, Viral metabolism, Molecular Sequence Data, RNA, Viral metabolism, Transcription, Genetic, Viral Proteins chemistry, Viral Proteins genetics, Bacillus virology, Bacillus Phages genetics
Abstract: Continuous research spanning more than three decades has made the Bacillus bacteriophage phi29 a paradigm for several molecular mechanisms of general biological processes, such as DNA replication, regulation of transcription, phage morphogenesis, and phage DNA packaging. The genome of bacteriophage phi29 consists of a linear double-stranded DNA (dsDNA), which has a terminal protein (TP) covalently linked to its 5' ends. Initiation of DNA replication, carried out by a protein-primed mechanism, has been studied in detail and is considered to be a model system for the protein-primed DNA replication that is also used by most other linear genomes with a TP linked to their DNA ends, such as other phages, linear plasmids, and adenoviruses. In addition to a continuing progress in unraveling the initiation of DNA replication mechanism and the role of various proteins involved in this process, major advances have been made during the last few years, especially in our understanding of transcription regulation, the head-tail connector protein, and DNA packaging. Recent progress in all these topics is reviewed. In addition to phi29, the genomes of several other Bacillus phages consist of a linear dsDNA with a TP molecule attached to their 5' ends. These phi29-like phages can be divided into three groups. The first group includes, in addition to phi29, phages PZA, phi15, and BS32. The second group comprises B103, Nf, and M2Y, and the third group contains GA-1 as its sole member. Whereas the DNA sequences of the complete genomes of phi29 (group I) and B103 (group II) are known, only parts of the genome of GA-1 (group III) were sequenced. We have determined the complete DNA sequence of the GA-1 genome, which allowed analysis of differences and homologies between the three groups of phi29-like phages, which is included in this review.
Published: 2001
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47. The switch from early to late transcription in phage GA-1: characterization of the regulatory protein p4G.

Author: Horcajadas JA, Monsalve M, Rojo F, and Salas M
Subjects: Amino Acid Sequence, Base Sequence, Binding Sites, Cloning, Molecular, DNA chemistry, DNA genetics, DNA metabolism, DNA Footprinting, DNA-Binding Proteins genetics, DNA-Binding Proteins isolation & purification, DNA-Directed RNA Polymerases metabolism, Dimerization, Genes, Viral genetics, Molecular Sequence Data, Osmolar Concentration, Promoter Regions, Genetic genetics, Repressor Proteins antagonists & inhibitors, Repressor Proteins metabolism, Sequence Homology, Amino Acid, Transcription Factors genetics, Transcription Factors isolation & purification, Viral Proteins genetics, Viral Proteins isolation & purification, Bacillus Phages genetics, DNA-Binding Proteins metabolism, Gene Expression Regulation, Viral, Transcription Factors metabolism, Transcription, Genetic genetics, Viral Proteins metabolism
Abstract: The transcription program of the Bacillus phage GA-1, a distant relative of phage Phi29, has been studied. Transcription of the GA-1 genome occurred in two stages, early and late. Early genes were expressed from two promoters equivalent to the Phi29 A2b and A2c promoters, whereas late transcription started at a site equivalent to the Phi29 late A3 promoter. The activity of the GA-1 early A2b and A2c promoters diminished 10 minutes after infection, a time at which expression of the late promoter increased significantly. The switch from early to late transcription required protein synthesis, suggesting the need for viral protein(s). An open reading frame was found in the GA-1 genome coding for a protein showing a 53 % similarity to Phi29 regulatory protein p4, and was named p4G. In Phi29, protein p4 represses the early A2b and A2c promoters and activates the late A3 promoter by recruiting RNA polymerase to it. A binding site for protein p4Gwas localized upstream from the GA-1 late A3 promoter, overlapping with the early A2b promoter. In vitro, protein p4Gprevented the binding of RNA polymerase to the GA-1 early A2b promoter but, unlike in Phi29, had no effect on the expression of the late A3 promoter: RNA polymerase could efficiently bind and initiate transcription from the A3 promoter in the absence of protein p4G. Therefore, activation of late transcription occurs differently in GA-1 and Phi29. We propose that protein p4Gis an anti-repressor which inhibits the binding to the late promoter of an unknown repressor factor present in the host strain., (Copyright 1999 Academic Press.)
Published: 1999
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