1. FAST-EM array tomography: a workflow for multibeam volume electron microscopy.
- Author
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Kievits AJ, Duinkerken BHP, Lane R, de Heus C, van Beijeren Bergen En Henegouwen D, Höppener T, Wolters AHG, Liv N, Giepmans BNG, and Hoogenboom JP
- Abstract
Elucidating the 3D nanoscale structure of tissues and cells is essential for understanding the complexity of biological processes. Electron microscopy (EM) offers the resolution needed for reliable interpretation, but the limited throughput of electron microscopes has hindered its ability to effectively image large volumes. We report a workflow for volume EM with FAST-EM, a novel multibeam scanning transmission electron microscope that speeds up acquisition by scanning the sample in parallel with 64 electron beams. FAST-EM makes use of optical detection to separate the signals of the individual beams. The acquisition and 3D reconstruction of ultrastructural data from multiple biological samples is demonstrated. The results show that the workflow is capable of producing large reconstructed volumes with high resolution and contrast to address biological research questions within feasible acquisition time frames., Competing Interests: Competing interests: A.J.K., B.H.P.D., R.L, C.H., D.B.B.H., T.H., A.H.G.W., N.L., and B.N.G.G. declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. FAST-EM is a product of Delmic BV, J.P.H. is co-founder of and shareholder in Delmic BV., (© 2024 the author(s), published by De Gruyter on behalf of Thoss Media.)
- Published
- 2024
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