Search

Vascular smooth muscle cells (VSMCs), which provides structural integrity and regulates the diameter of vasculature, are of great potential for modeling vascular-associated diseases and tissue engineering. Here, we presented a detailed comparison of differentiating human pluripotent stem cells (hPSCs) into VSMCs (hPSCs-VSMCs) in four different culture methods, including 2-dimensional (2D) culture, 3-dimensional (3D) PNIPAAm-PEG hydrogel culture, 3-dimensional (3D) alginate hydrogel culture, and transferring 3-dimensional alginate hydrogel culture to 2-dimensional (2D) culture. Both hydrogel-based culture methods could mimic in vivo microenvironment to protect cells from shear force, and avoid cells agglomeration, resulting in the extremely high culture efficiency (e.g., high viability, high purity and high yield) compared with 2D culture. We demonstrated hPSC-VSMCs produced from hydrogel-based culture methods had better contractile phenotypes and the potential of vasculature formation. The transcriptome analysis showed the hPSC-VSMCs derived from hydrogel-based culture methods displayed more upregulated genes in vasculature development, angiogenesis and blood vessel development, extracellular matrix compared with 2D culture. Taken together, hPSC-VSMCs produced from hydrogel-based culture system could be applied in various biomedical fields, and further indicated the suitable development of alginate hydrogel for industrial production by taking all aspects into consideration.

Abstract Zinc finger CCCH-type containing 15 (ZC3H15), a highly conserved eukaryotic protein, which was associated with several cellular processes and was ubiquitously expressed in various human tissues. Recent studies indicated that ZC3H15 was involved in tumorigenesis and may be a potential biomarker in hepatocellular carcinoma (HCC) and acute myeloid leukemia (AML). However, the biological function and molecular mechanism of ZC3H15 in gastric cancer (GC) have not been studied. In this study, we revealed that ZC3H15 was highly expressed in GC and high ZC3H15 expression was closely linked to poor survival of patients with GC. We found that ZC3H15 promoted cell proliferation, migration, and invasion by increasing c-Myc expression. Next, we found that ZC3H15 could modulate c-Myc protein stability by suppressing the transcription of FBXW7, which was mainly responsible for c-Myc degradation. Moreover, silencing of FBXW7 in ZC3H15-knockdown GC cells could partly abrogate the effects induced by ZC3H15 downregulation. Taken together, our data unearth the important roles of ZC3H15 in GC development and suggest that ZC3H15 may be a potential target for the treatment of GC.

Abstract Zinc finger CCCH-type containing 15 (ZC3H15), a highly conserved protein involved in several cellular processes, which was responsible for tumorigenesis and may be a promising marker in myeloid leukemia (AML) and hepatocellular carcinoma (HCC). However, little is known about the biological significance and molecular mechanisms of ZC3H15 in GBM. In this study, we revealed that ZC3H15 was overexpressed in GBM and high ZC3H15 expression was associated with poor survival of patients with GBM. We found that ZC3H15 promoted the proliferation, migration, invasion, and tumorigenesis of GBM cells by activating the EGFR signaling pathway. We also revealed that ZC3H15 reduced EGFR ubiquitination, which was responsible for EGFR protein stabilization. In addition, we demonstrated that ZC3H15 inhibited the transcription of CBL, which was an E3 ubiquitin ligase for EGFR proteasomal degradation. And silencing of CBL could partly abrogate the inhibitory effects on cell proliferation, migration, and invasion of GBM cells induced by ZC3H15 knockdown. Thus, our research revealed the important roles of ZC3H15 in GBM development and provided a brand-new insight for improving the treatment of GBMs.

Analysis of the coseismic differential response of adjacent monitoring wells is an important way to study the coseismic response mechanism of groundwater. On February 3, 2020, the Qingbaijiang earthquake caused the abnormal changes of groundwater levels and water quality in the monitoring wells of Longquanshan. In this paper, the coseismic differential response characteristics of two adjacent monitoring wells are analyzed by using the automatic monitoring data of various hydrochemical components and water levels. Based on the coupling response characteristics of hydrochemical components and water levels, the mechanism of differential response of hydrochemical components and water levels is discussed. In the case of the same energy density, the water level variation amplitude of well ZK1 is larger than that of well ZK6, which indicates that ZK1 is more sensitive to the earthquake response than ZK6. The earthquake mainly caused the discharge of the groundwater (Eh0) from the first aquifer (high concentration of Cl−, TDS, and pH) to ZK6. The discharge and mixing of water from different aquifers into the wells are the reason for the different response of chemical components of groundwater in these two wells. According to the trends of chemical components and water levels after the earthquake, rock mass under seismic action of undrained volume change is the main cause of groundwater water status step change, that is, plastic deformation and permanent change in permeability occurred in the second aquifer around well ZK1, and no permanent change in permeability but elastic deformation occurred in rock mass around well ZK6.

Ubiquitination and SUMOylation, which are posttranslational modifications, play prominent roles in regulating both protein expression and function in cells, as well as various cellular signal transduction pathways. Metabolic reprogramming often occurs in various diseases, especially cancer, which has become a new entry point for understanding cancer mechanisms and developing treatment methods. Ubiquitination or SUMOylation of protein substrates determines the fate of modified proteins. Through accurate and timely degradation and stabilization of the substrate, ubiquitination and SUMOylation widely control various crucial pathways and different proteins involved in cancer metabolic reprogramming. An understanding of the regulatory mechanisms of ubiquitination and SUMOylation of cell proteins may help us elucidate the molecular mechanism underlying cancer development and provide an important theory for new treatments. In this review, we summarize the processes of ubiquitination and SUMOylation and discuss how ubiquitination and SUMOylation affect cancer metabolism by regulating the key enzymes in the metabolic pathway, including glucose, lipid and amino acid metabolism, to finally reshape cancer metabolism.

Purpose. Magnetic resonance imaging (MRI) has a high sensitivity for differentiating between malignant and non-malignant breast lesions but is sometimes limited due to its low specificity. Here, we performed a meta-analysis to evaluate the diagnostic performance of mean kurtosis (MK) and mean diffusivity (MD) values in magnetic resonance diffusion kurtosis imaging (DKI) for benign and malignant breast lesions. Methods. Original articles on relevant topics, published from 2010 to 2019, in PubMed, EMBASE, and WanFang databases were systematically reviewed. According to the purpose of the study and the characteristics of DKI reported, the diagnostic performances of MK and MD were evaluated, and meta-regression was conducted to explore the source of heterogeneity. Results. Fourteen studies involving 1,099 (451 benign and 648 malignant) lesions were analyzed. The pooled sensitivity, pooled specificity, positive likelihood ratio, and negative likelihood ratio for MD were 0.84 (95% confidence interval (CI), 0.81-0.87), 0.83 (95% CI, 0.79-0.86), 4.44 (95% CI, 3.54-5.57), and 0.18 (95% CI, 0.13-0.26), while those for MK were 0.89 (95% CI, 0.86-0.91), 0.86 (95% CI, 0.82-0.89), 5.72 (95% CI, 4.26-7.69), and 0.13 (95% CI, 0.09-0.19), respectively. The overall area under the curve (AUC) was 0.91 for MD and 0.95 for MK. Conclusions. Analysis of the data from 14 studies showed that MK had a higher pooled sensitivity, pooled specificity, and diagnostic performance for differentiating between breast lesions, compared with MD.

The effect of corrosion time on the mechanical behavior of 5083/6005A welded joints in a 3.5% NaCl + 0.01 mol/L NaHSO3 solution was evaluated via scanning electron microscopy (SEM), polarization curve analysis, and X-ray photoelectron spectroscopy (XPS). The prediction model of fatigue life after corrosion was established based on the experimental results and the theory of fracture mechanics, and the formula for the effect of corrosion time on lifespan was determined. The results show that with increasing corrosion time, the corrosion of the sample becomes increasingly severe, and the elongation and fatigue life of the 5083/6005A welded joints decrease significantly. The corrosion resistance of the 5083/6005A welded joints decreases with increasing corrosion time because the corrosive medium promotes the destruction of the oxide film and thereby reduces the corrosion resistance. The corrosion products of the 5083/6005A welded joints are Al(OH)3 and AlCl3.

Small ribosomal protein subunit S7 (RPS7) has been reported to be associated with various malignancies, but the role of RPS7 in ovarian cancer remains unclear. In this study, we found that silencing of RPS7 by a specific shRNA promoted ovarian cancer cell proliferation, accelerated cell cycle progression, and slightly reduced cell apoptosis and response to cisplatin treatment. Knockdown of RPS7 resulted in increased expression of P85α, P110α, and AKT2. Although the basal levels of ERK1/2, MEK1/2, and P38 were inconsistently altered in ovarian cancer cells, the phosphorylated forms of MEK1/2 (Ser217/221), ERK1/2 (Thr202/Tyr204), JNK1/2 (Thr183/Tyr185), and P38 (Thr180/Tyr182) were consistently reduced after RPS7 was silenced. Both the in vitro anchorage-independent colony formation and in vivo animal tumor formation capability of cells were enhanced after RPS7 was depleted. We also showed that silencing of RPS7 enhanced ovarian cancer cell migration and invasion. In sum, our results suggest that RPS7 suppresses ovarian tumorigenesis and metastasis through PI3K/AKT and MAPK signal pathways. Thus, RPS7 may be used as a potential marker for diagnosis and treatment of ovarian cancer.

Background: Collagen type VIII α 1 chain (COL8A1), a collagen type VIII protein, has been suggested to exert various functions in progression of multiple cancers. However, the effect of COL8A1 in papillary thyroid cancer (PTC) has not been elucidated. Methods: The Cancer Genome Atlas (TCGA) databases were applied to investigate the COL8A1 expression and its clinical significance in PTC. The COL8A1 expression level was further validated using Gene Expression Omnibus (GEO) data and clinical paired PTC tissues. Additionally, the Kaplan–Meier curve was used to analyze the prognosis. The cell’s migrative and invasive abilities were evaluated by wound healing assay and Transwell assay. CCK8 assays were used to evaluate the proliferation of PTC cells. Western blotting was conducted to explore the potential mechanisms involved in the pro-tumor role of COL8A1. The correlation between immune cell infiltration and COL8A1 was analyzed using the Tumor Immune Estimation Resource (TIMER) database and the single-sample GSEA (ssGSEA) method. Results: We found that COL8A1 was upregulated in PTC (P < 0.05). High COL8A1 expression level was significantly associated with advanced T stage (P < 0.01), N stage (P < 0.001) and poor prognosis (P = 0.0142) in PTC. Furthermore, cell migration and invasion were significantly reduced following COL8A1 knockdown (P < 0.001). Mechanistic studies demonstrated that the epithelial-to-mesenchymal transition (EMT) related proteins (FN1, MMP9, MMP7, ZEB2 and Twist1) and phosphorylation of AKT and ERK were obviously down-regulated after COL8A1 knockdown (P < 0.01). Moreover, COL8A1 expression was correlated with immune cell infiltration. Conclusion: Our study demonstrates that COL8A1 may function as an oncogene and a potential prognostic biomarker for PTC patients.

Background: Transforming growth factor beta receptor III (TGFBR3) has been shown to play a tumor-suppressive role in a variety of cancers. However, its role in papillary thyroid cancer (PTC) remains unknown. Method: TGFBR3 expression levels in PTC were analyzed utilizing The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Edu, wound healing, and Transwell assays were used to evaluate cell proliferation, migration, and invasion. Transcriptome sequencing, quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), and Western blotting were used to detect the underlying mechanism of TGFBR3 in PTC progression. Result: This study demonstrated that TGFBR3 expression was significantly down-regulated in PTC compared to normal thyroid tissues. Low expression of TGFBR3 was associated with poor prognosis of patients with PTC. Furthermore, TGFBR3 expression positively correlated with thyroid differentiation score. In investigating the biological impact of TGFBR3 overexpression in PTC cell lines, we found that the proliferation, migration, and invasion of PTC cells were significantly inhibited in response to TGFBR3 overexpression. Moreover, we also demonstrated that overexpression of TGFBR3 inhibited the PI3K/AKT pathway and epithelial-mesenchymal transformation processes. Lastly, TGFBR3 expression was found to be involved in tumor immune infiltration, highlighting its potential influence on immune dynamics within the tumor microenvironment in PTC. Conclusion: TGFBR3 plays a tumor-suppressive role in PTC progression by inhibiting the PI3K/AKT pathway and epithelial mesenchymal transformation.

Abstract Ruthenium (Ru) is widely recognized as a low-cost alternative to iridium as anode electrocatalyst in proton-exchange membrane water electrolyzers (PEMWE). However, the reported Ru-based catalysts usually only operate within tens of hours in PEMWE because of their intrinsically high reactivity of lattice oxygen that leads to irrepressible Ru leaching and structural collapse. Herein, we report a design concept by employing large-sized and acid-resistant lattice lead (Pb) as a second element to induce a pinning effect for effectively narrowing the moving channels of oxygen atoms, thereby lowering the reactivity of lattice oxygen in Ru oxides. The Pb-RuO2 catalyst presents a low overpotential of 188 ± 2 mV at 10 mA cm−2 and can sustain for over 1100 h in an acid medium with a negligible degradation rate of 19 μV h−1. Particularly, the Pb-RuO2-based PEMWE can operate for more than 250 h at 500 mA cm−2 with a low degradation rate of only 17 μV h−1. Experimental and theoretical calculation results reveal that Ru-O covalency is reduced due to the unique 6s−2p−4d orbital hybridization, which increases the loss energy of lattice oxygen and suppresses the over-oxidation of Ru for improved long-term stability in PEMWE.

Abstract Background It is well-established that understanding the mechanism of prostate cancer (PCa)-associated metastasis is paramount for improving its prognosis. Metastasis is known to involve the communication between tumor-associated macrophages (TAMs) and tumor cells. Exosomes are crucial in mediating this intercellular communication within the tumor microenvironment. Nonetheless, the role of exosomal proteins in PCa metastasis is not yet fully understood. Here, we investigated the mechanisms of prostate cancer-derived exosomal PSM-E on regulating macrophage M2 polarization to suppress tumor invasion and metastasis. Methods PSM-E levels in exosomes were detected by transmission electron microscopy and Western blotting analysis. The diagnostic value of urine-derived exosomal PSM-E in PCa were evaluated by LC-MS/MS, correlation analysis, and ROC curves analysis. The mechanisms underlying the inhibitory effect of exosomal PSM-E on the M2 polarization of macrophages was investigated by co-IP, IHC staining, and PCa tumorigenesis model, etc. Results We revealed that exosomal PSM-E is upregulated in exosomes derived from the serum and urine of PCa patients. Clinically, an elevated exosomal PSM-E expression in urine is significantly correlated with an advanced pathological tumor stage and a high Gleason score. Our research also revealed that exosomal PSM-E inhibits prostate cancer cell proliferation, invasion, and metastasis by suppressing macrophage polarization in vitro and in vivo. Furthermore, we provided compelling evidence that exosomal PSM-E inhibits M2 polarization of macrophages by recruiting RACK1 and suppressing the FAK and ERK signaling pathways, consequently suppressing PCa invasion and metastasis. Furthermore, we found that the protease-associated domain of PSM-E and the fourth tryptophan-aspartate repeat of RACK1 are crucial for the interaction between PSM-E and RACK1. Conclusions Notably, exosomes carrying PSM-E from PCa urine could potentially serve as a biomarker for PCa, and targeting exosomal PSM-E may represent a strategy for preventing tumor progression in this patient population.

Weathered coal has low calorific value, single utilization mode and environmental pollution. In order to broaden the utilization way of weathered coal, realize resource utilization and environmental protection, dark fermentation of weathered coal was carried out to produce biological hydrogen. Naturally weathered coals from three mining areas of Jincheng and Taiyuan in Shanxi Province and Wuhai in Inner Mongolia were selected, and the coal seam mine water was used as the source of bacteria. The feasibility of biological hydrogen production from weathered coal and the key metabolites and pathways were revealed by means of biological hydrogen production test, GC-MS, three-dimensional fluorescence and metagenomics. The results show that weathered coal in different mining areas can be converted into bio-hydrogen, and the hydrogen production of Wuhai weathered coal is the largest (10.26 mL/g), which is much higher than that of Jincheng weathered coal (5.22 mL/g). The hydrogen production system of weathered coal is in an acidic environment, and pH and COD mass concentration have certain regular changes. In the process of hydrogen production from weathered coal, the liquid organic matter is mainly composed of acids (acetic acid, propionic acid, valeric acid and butyric acid) and alcohols (2,3-butanediol, sugar alcohol and (S)-1,2-propanediol). The soluble organic matter is mainly composed of humic acid and tryptophan protein organic matter, and with the progress of fermentation, a variety of organic matter is consumed and utilized. In the process of hydrogen production from weathered coal, microorganisms are mainly dominated by Pseudomonadota and Citrobacter, and hydrogen is mainly produced by decomposing and utilizing organic acids (acetic acid, propionic acid, butyric acid, lactic acid). In the process of hydrogen production from weathered coal, the acetic acid metabolic pathway is dominated by the glycolysis pathway. The organic matter is decomposed by bacteria to produce pyruvate and then used by hydrogen-producing bacteria. Among them, alanine kinase and aldehyde ketone dehydrogenase play an important role in the process of cell metabolism to produce hydrogen. The research results reveal the potential mechanism of hydrogen production from weathered coal and provide a theoretical reference for the effective utilization of weathered coal.

IntroductionThe accurate and rapid detection of ginseng fruits in natural environments is crucial for the development of intelligent harvesting equipment for ginseng fruits. Due to the complexity and density of the growth environment of ginseng fruits, some newer visual detection methods currently fail to meet the requirements for accurate and rapid detection of ginseng fruits. Therefore, this study proposes the YOLO-Ginseng detection method.MethodsFirstly, this detection method innovatively proposes a plug-and-play deep hierarchical perception feature extraction module called C3f-RN, which incorporates a sliding window mechanism. Its unique structure enables the interactive processing of cross-window feature information, expanding the deep perception field of the network while effectively preserving important weight information. This addresses the detection challenges caused by occlusion or overlapping of ginseng fruits, significantly reducing the overall missed detection rate and improving the long-distance detection performance of ginseng fruits; Secondly, in order to maintain the balance between YOLO-Ginseng detection precision and speed, this study employs a mature channel pruning algorithm to compress the model.ResultsThe experimental results demonstrate that the compressed YOLO-Ginseng achieves an average precision of 95.6%, which is a 2.4% improvement compared to YOLOv5s and only a 0.2% decrease compared to the uncompressed version. The inference time of the model reaches 7.4ms. The compressed model exhibits reductions of 76.4%, 79.3%, and 74.2% in terms of model weight size, parameter count, and computational load, respectively.DiscussionCompared to other models, YOLO-Ginseng demonstrates superior overall detection performance. During the model deployment experiments, YOLO-Ginseng successfully performs real-time detection of ginseng fruits on the Jetson Orin Nano computing device, exhibiting good detection results. The average detection speed reaches 24.9 fps. The above results verify the effectiveness and practicability of YOLO-Ginseng, which creates primary conditions for the development of intelligent ginseng fruit picking equipment.

Abstract Background Previous research has revealed a negative association between social support and procrastination. However, few studies have investigated the mechanism underlying this relationship among vocational college students. Objective Based on the social cognitive theory, this study was intended to investigate the multiple mediating effects of self-efficacy and resilience on the relationship between social support and procrastination among vocational college students. Methods This study employed a cross-sectional design involving a sample of 1,379 students from a vocational college in China. Data were collected using the General Procrastination Scale, the Multidimensional Scale of Perceived Social Support, the General Self-Efficacy Scale, and the Resilience Scale-14. The PROCESS macro for SPSS was used to examine the multiple mediation model. Results Our findings indicate significant negative correlations between social support, self-efficacy, resilience, and procrastination. The multiple mediation analysis showed that social support did not have a significant direct impact on procrastination. Instead, the relationship between social support and procrastination was fully mediated by self-efficacy (indirect effect: -0.017; 95% CI: -0.032, -0.004) and resilience (indirect effect: -0.047; 95% CI: -0.072, -0.025), and sequentially mediated by both factors (indirect effect: -0.013; 95% CI: -0.020, -0.007). Conclusions The results emphasise the importance of enhancing self-efficacy and resilience in initiatives aimed at preventing and intervening in case of procrastination among vocational college students. Additionally, strengthening social support may also be crucial to preventing or reducing procrastination among this population.

Adding corn straw to the anaerobic fermentation system of coal can significantly increase the gas production of coal-to-biomethane. However, the changes in substrate and microbial metabolism accompanying this combined fermentation are rarely studied. The Scanning Electron Microscope (SEM) test was utilized to analyze the microbial adhesion characteristics of coal and corn straw, while the X-ray Photoelectron Spectroscopy (XPS) test examined the changes in surface elements of coal. The results indicated that the optimal ratios of lean coal, weakly caking coal, and coking coal to corn straw were 2∶1, 2∶1, and 3∶1, respectively, and the corresponding methane yields were 17.28 mL/g, 12.51 mL/g, and 14.88 mL/g. The promotional effect of co-fermentation of different rank coal and straw was observed in the order of lean coal > coking coal > weakly caking coal. Microbial identification revealed that Sphaerochaeta and Proteiniphilum were the dominant bacterial genera in the mixed fermentation system, comprising 35.95% and 24.36% of the population, respectively. Additionally, Methanosarcina, Methanobacterium, and Methanoculleus emerged as the dominant archaea, constituting 49.71%, 31.83%, and 9.87%, respectively. In comparison with coking coal and weakly caking coal, the bacterial and archaeal genera, along with their associated gene functions responsible for carbohydrate metabolism in the combined fermentation broth of lean coal, coking coal, and weakly caking coal, were found to be dominant. This observation aligns with the principles governing methane production. Notably, in mixed substrate fermentation, as coal rank increased, bacterial presence on the coal surface gradually declined, while a substantial bacterial population consistently adhered to the surface of corn straw, indicating the latter's sustained advantage in degradation. After co-fermentation, the relative content of C element on the surface of coking coal and weakly caking coal decreased more significantly than that of lean coal, while the total relative content of oxygen-containing organic carbon ( C—O, C=O and O—C=O ) increased significantly, indicating that co-fermentation can promote the growth and metabolism of bacteria, so that more organic carbon can be converted into biological methane. The research results identified the effect of co-fermentation of different rank coal and corn straw at the micro level, which has reference significance for revealing the internal mechanism of co-fermentation of coal and corn straw.

Background: Filamin A (FLNA) is a member of the filamin family and has been found to be critical for the progression of several cancers. However, its biological function in papillary thyroid cancer (PTC) remains largely unexplored. Methods: Data from The Cancer Genome Atlas (TCGA) databases were utilized to analyze the FLNA expression level and its influence on the clinical implications of patients with PTC. Gene Expression Omnibus (GEO) and qRT-PCR was used to verify the expression levels of FLNA in PTC. Kaplan–Meier survival analysis was conducted to evaluate the prognostic value of FLNA in PTC. Transwell assays and wound healing were performed to examine the biological function of FLNA knockdown in PTC cells. Gene set enrichment analysis (GSEA) and Western blotting were conducted to investigate the potential mechanisms underlying the role of FLNA in PTC progression. In addition, the relationship between FLNA expression and the tumor immune microenvironment (TME) in PTC was explored. Results: FLNA was significantly upregulated in PTC tissues. High expression levels of FLNA was correlated with advanced TNM stage, T stage, and N stage, as well as poor disease-free interval (DFI) and progression-free interval (PFI) time in PTC patients. Moreover, we found that FLNA knockdown inhibited the migration and invasion of PTC cells. Mechanistically, FLNA knockdown inhibited epithelial–mesenchymal transition (EMT) in PTC and affected the activation of the FAK/AKT signaling pathway. In addition, FLNA expression was associated with TME in PTC. Conclusion: FLNA may be regarded as a new therapeutic target for PTC patients.

Abstract With growing recognition of the importance of community engagement in addressing public health challenges, its role in promoting healthy behaviors and preventing infectious diseases has gained attention. However, vaccination coverage remains a significant concern in many developing countries. While previous studies have linked community engagement to positive health outcomes, there is a gap in understanding its influence on individual vaccination choices, particularly in the context of developing countries. Utilizing data from the 2021 Chinese General Social Survey (CGSS), this study examines the impact of community engagement on COVID-19 and flu vaccination uptake among 7281 individuals. Community engagement, measured by community vaccination notifications, serves as the key independent variable. The study employs Ordinary Least Squares (OLS) regression and Propensity Score Matching (PSM) methods to analyze the relationship between community engagement and vaccination behavior. The analysis reveals a positive association between community engagement and vaccination rates. Specifically, individuals receiving notifications were more likely to get the COVID-19 vaccine compared to non-recipients (vaccination rates: 100% vs. 53.3%), and flu vaccination rates were also significantly higher among those notified (2.7% vs. 1.9%). Mechanism analysis suggests that individuals receiving community notifications are more aware of the benefits of vaccination, leading to higher vaccination rates among this group. This study underscores the effectiveness of community engagement strategies in promoting positive vaccination behavior among individuals in China. By enhancing awareness and trust in immunization, community engagement initiatives play a crucial role in shaping health behaviors and improving vaccination uptake. These findings emphasize the importance of integrating community engagement approaches into public health interventions to address vaccination challenges.

ABSTRACT Background The clinical impact of obesity on postoperative outcomes of patients undergoing thyroidectomy remains controversial. Methods Patients aged ≥ 18 years who were diagnosed with thyroid malignancy and underwent thyroidectomy between 2016 and 2020 were included, and divided into two groups: patients with body mass index (BMI)

In order to find out the internal reasons for the difference in biological methane production between weathered coal and lignite, weathered coal and lignite were selected respectively, and the enriched and domesticated bacterial liquid was used as the source of bacteria. The internal mechanism of the difference in biological gas production between weathered coal and lignite was analyzed by biological gas production simulation, infrared spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy and 16 S rRNA test. The results showed that the biogas production potential of lignite (7.63 mL/g) was higher than that of weathered coal (3.24 mL/g). Compared with weathered coal, in the process of gas production, various groups of lignite fall off obviously, aromatic substances are more converted into other substances with small molecular weight, and phenolic carbon or ether carbon (C—O) oxidation is more obvious, which is conducive to the formation of more small molecular organic acids. Compared with weathered coal, lignite has more serious surface erosion and more pore cracks in the process of biological gas production. The diversity of bacteria and archaea in lignite is lower than that in weathered coal, but the main functional flora accounts for a large proportion. The proportion of Macellibacteroides and Lysinibacillus in bacteria reaches 68.05%, and the proportion of Methanosarcina and Methanobacterium in archaea reaches 89.99%, which is much larger than that of weathered coal. It plays a positive role in the degradation and utilization of organic matter in coal and can provide raw materials for subsequent methanogens. Therefore, lignite is more conducive to the utilization of microbial products to produce methane. Methane metabolism type of weathered coal is methyl nutrition hydrogen nutrition type different from lignite. Microorganisms in lignite can make better use of acetic acid as a carbon source, glucose and glycogen can be effectively degraded into pyruvate, strong oxidation (reduction), and produce ATP for biochemical reactions. The research results provide a theoretical reference for the mechanism of gas production difference between weathered coal and lignite, and provide practical significance for the future biomethanation utilization of weathered coal and lignite.