106 results on '"Hongying Cao"'
Search Results
2. Predictive value of combining urinary N-acetyl-β-D-glucosaminidase and serum homocysteine for contrast-induced nephropathy in patients after percutaneous coronary intervention
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Yiling Zhai, Changjun Luo, Nianying Qin, Hongying Cao, Chunyang Dong, Zhou Huang, Dongling Huang, Fan Wang, Wanxia Wei, Jincheng Li, Jie Yang, Xueling Lu, Zhengzhuang Huang, and Wei Wang
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N-acetyl-β-D-glucosaminidase ,homocysteine ,biomarker ,percutaneous coronary intervention ,contrast-induced nephropathy ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundContrast-induced nephropathy (CIN) can lead to serious complications following percutaneous coronary intervention (PCI). Urine N-Acetyl-β-D-glucosaminidase (uNAG) and serum homocysteine (sHCY) are both potential predictors for CIN detection, but their combination has not been explored. We aimed to combine uNAG and sHCY as predictors for the early detection of CIN and for prognosis prediction in patients after PCI.MethodsA total of 232 consecutive patients who underwent PCI at a university hospital were recruited for this study. According to the European Society of Urology and Reproduction (ESUR) criterion, CIN is defined as an elevation of serum creatinine (sCr) by ≥25% or ≥0.5 mg/dl from baseline within 48 h. We assessed the use of individual biomarkers (uNAG and sHCY) measured around PCI and their combinations for CIN detection and prognosis prediction. Receiver operating characteristic curves (ROC) and area under the curve (AUC) were used to evaluate the predictive efficiency of potential predictors.ResultsIn total, 54 (23.28%) patients developed CIN. Concentrations of uNAG and sHCY increased significantly in CIN subjects (p
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- 2024
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3. Excitatory purinergic and cholinergic expression changed in a partial bladder outlet obstruction-induced overactive bladder rat model
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Jingyi Huang, Hongliang Li, Yao Zhang, Jiaye Liu, Hongying Cao, and Yongling Long
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Medicine ,Science - Abstract
Abstract Overactive bladder (OAB) is a common, long-term symptom complex with a high prevalence in women worldwide. OAB has caused a social burden, and effective treatments are urgently needed. However, the pathogenesis of OAB has yet to be elucidated. Model rats underwent bladder outlet obstruction surgery. In the 2nd, 3rd, and 4th weeks after surgery, metabolic cages were used to detect the 12 h urine volume of rats in the sham and model groups. The urodynamic parameters bladder leak point pressure (BPLL), maximum voiding pressure (MVP), residual volume (RV), maximum bladder capacity (MBC), bladder compliance (BC), voided efficiency (VE), and non-voiding contractions (NVCs) were also detected. Moreover, the contractile responses of isolated detrusor muscles to electrical and carbachol stimulation were examined at the abovementioned time points. At the 4th week after surgery, the bladders of both groups were obtained for hematoxylin–eosin (H&E) and Masson’s trichrome staining. Real-time qPCR and Western blot were performed to quantify the expression of choline acetyltransferase (ChAT) and solute carrier family 17 member 9 (SLC17A9). At week 4, compared with the sham group, the 12 h urine volume of PBOO group increased significantly. The BLPP, MVP, VE, MBC, and NVCs increased significantly, and the VE was significantly reduced in 4-week PBOO group. The contractile responses of isolated detrusor muscles to electrical and carbachol stimulation significantly increased in 4-week PBOO group. In the 4-week PBOO group, the bladder wall and the ratio of bladder muscle to collagen within the bladder smooth muscle layer wall were significantly higher than those in the sham group. ChAT and SLC17A9 mRNA and protein expression in the OAB model rats significantly increased. At 4 weeks after PBOO, the OAB model was successfully established. The gene and protein expression levels of ChAT and SLC17A9 increased in the bladder of the OAB model, suggesting that OAB may be related to increased excitatory purinergic and cholinergic expression.
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- 2023
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4. Corrigendum: Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats
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Wanyu Chen, Lu Liao, Zitong Huang, Yulin Lu, Yukang Lin, Ying Pei, Shulin Yi, Chen Huang, Hongying Cao, and Bo Tan
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patchouli alcohol ,irritable bowel syndrome with diarrhea ,colonic longitudinal muscle myenteric plexus ,excitatory neurons ,intestinal motility ,Therapeutics. Pharmacology ,RM1-950 - Published
- 2024
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5. Impaired colonic motility in high-glycemic diet-induced diabetic mice is associated with disrupted gut microbiota and neuromuscular function
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Ying Pei, Rui Wang, Wanyu Chen, Shulin Yi, Chen Huang, Shaochan Liang, Hongying Cao, Yifei Xu, and Bo Tan
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diabetes ,obesity ,constipation ,colonic motility ,gut microbiota ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
Background: Similar to the high-fat diet (HFD), the high-glycemic diet (HGD) contributes to the development and progression of type 2 diabetes mellitus (T2DM). However, the effect of HGD on gastrointestinal motility in T2DM and its u nderlying mechanisms remain unclear. Methods: Thirty C57BL/6J mice were randomly designated into the normal-feeding diet (NFD) group, HFD group, and HGD group. The plasma glucose, plasma insulin, and gastrointestinal motility were examined. Meanwhile, the tension of isolated colonic smooth muscle rings was calculated, and the gut microbiota was analyzed by 16s rDNA high-throughput sequencing. Result: After 16 weeks of HGD feeding, obesity, hyperglycemia, insulin resistance, and constipation were observed in HGD mice. Autonomic contraction frequency of the colonic neuromuscular system and electrical field stimulation-induced contractions were reduced in HGD mice. On the contrary, neuronal nitric oxide synthase activity and neuromuscular relaxation were found to be enhanced. Finally, gut microbiota analysis revealed that Rhodospirillaceae abundance significantly increased at the family level in HGD mice. At the genus level, the abundance of Insolitispirillum increased remarkably, whereas Turicibacter abundance decreased significantly in HGD mice. Conclusion: HGD induced constipation in obese diabetic mice, which we speculated that it may be related to neuromuscular dysmotility and intestinal microbiota dysbiosis.
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- 2023
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6. PROTACs in gastrointestinal cancers
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Yu Chen, Qingfan Yang, Jinrun Xu, Liyao Tang, Yan Zhang, Fukuan Du, Yueshui Zhao, Xu Wu, Mingxing Li, Jing Shen, Ruilin Ding, Hongying Cao, Wanping Li, Xiaobing Li, Meijuan Chen, Zhigui Wu, Chi Hin Cho, Yu Du, Qinglian Wen, and Zhangang Xiao
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PROTACs ,gastrointestinal cancers ,targeted protein degradation ,cancer treatment ,E3 ligases ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Proteolysis targeting chimera (PROTAC) presents a powerful strategy for targeted protein degradation (TPD). The heterobifunctional PROTAC molecule consists of an E3 ligase ligand covalently linked to a protein of interest (POI) via a linker. PROTAC can induce ubiquitinated proteasomal degradation of proteins by hijacking the ubiquitin-proteasome degradation system (UPS). This technique has the advantages of broad targeting profile, good cell permeability, tissue specificity, high selectivity, oral bioavailability, and controllability. To date, a growing number of PROTACs targeting gastrointestinal cancers have been successfully developed, and, in many cases, their POIs have been validated as clinical drug targets. To the best of our knowledge, 15 PROTACs against various targets are currently tested in clinical trials, and many more are likely to be added in the near future. Therefore, this paper details the mechanism, research progress, and application in clinical trials of PROTACs, and summarizes the research achievements related to PROTACs in gastrointestinal cancers. Finally, we discuss the advantages and potential challenges of PROTAC for cancer treatment.
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- 2022
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7. Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats
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Wanyu Chen, Lu Liao, Zitong Huang, Yulin Lu, Yukang Lin, Ying Pei, Shulin Yi, Chen Huang, Hongying Cao, and Bo Tan
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patchouli alcohol ,irritable bowel syndrome with diarrhea ,colonic longitudinal muscle myenteric plexus ,excitatory neurons ,intestinal motility ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background and Purpose: Irritable bowel syndrome (IBS) is usually associated with chronic gastrointestinal disorders. Its most common subtype is accompanied with diarrhea (IBS-D). The enteric nervous system (ENS) modulates major gastrointestinal motility and functions whose aberration may induce IBS-D. The enteric neurons are susceptible to long-term neurotransmitter level alterations. The patchouli alcohol (PA), extracted from Pogostemonis Herba, has been reported to regulate neurotransmitter release in the ENS, while its effectiveness against IBS-D and the underlying mechanism remain unknown.Experimental Approach: In this study, we established an IBS-D model in rats through chronic restraint stress. We administered the rats with 5, 10, and 20 mg/kg of PA for intestinal and visceral examinations. The longitudinal muscle myenteric plexus (LMMP) neurons were further immunohistochemically stained for quantitative, morphological, and neurotransmitters analyses.Key Results: We found that PA decreased visceral sensitivity, diarrhea symptoms and intestinal transit in the IBS-D rats. Meanwhile, 10 and 20 mg/kg of PA significantly reduced the proportion of excitatory LMMP neurons in the distal colon, decreased the number of acetylcholine (Ach)- and substance P (SP)-positive neurons in the distal colon and restored the levels of Ach and SP in the IBS-D rats.Conclusion and Implications: These findings indicated that PA modulated LMMP excitatory neuron activities, improved intestinal motility and alleviated IBS-induced diarrheal symptoms, suggesting the potential therapeutic efficacy of PA against IBS-D.
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- 2022
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8. Modified Cangfu Daotan decoction ameliorates polycystic ovary syndrome with insulin resistance via NF-κB/LCN-2 signaling pathway in inflammatory microenvironment
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Shuowen Liu, Yao Zhang, Fang Yang, Jingna Gu, Ruyue Zhang, Yingying Kuang, Wantong Mai, Chengbo Zheng, Yang Yu, Ruling Lu, Lei Zeng, Hongying Cao, and Yongling Long
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Lcn-2 ,NF-kB signaling pathway ,INSR/IRS-1/GLUT4 ,PCOS-IR ,Modified Cangfu Daotan decoction ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
This study explored the possible connection between the insulin resistance-targeting protein adipokine lipocalin-2 (LCN-2) and NF-κB signaling pathway in the inflammatory microenvironment in PCOS-IR model rats to determine the pharmacological mechanism of modified Cangfu Daotan decoction (MCDD) intervention for PCOS-IR. We used a high-fat diet (42 days) combined with letrozole (1 mg/kg/day, 42 days) to establish a PCOS-IR rat model. From the third week after modeling, the rats were given continuous administration of MCDD (high dose with 31.68 g/kg, medium dose with 15.84 g/kg, and low dose with 7.92 g/kg) for 28 days. Serum, ovarian tissue, liver, and adipose tissue were collected after the last gavage. Enzyme-linked immunosorbent assay, hematoxylin-eosin (HE) staining, Masson staining, qRT-PCR, and Western blot experiments were performed to detect various indicators. Our results showed that MCDD could reduce body weight and abdominal fat weight; restore normal estrous cycle and ovarian function; alleviate fatty liver; regulate HOMA-IR and OGTT index; reduce serum inflammatory factor levels, LCN-2 level, and gene expression; and regulate the insulin signal transduction and NF-κB pathways in PCOS-IR rats. Thus, MCDD may play a role in improving ovarian function in PCOS-IR rats by downregulating NF-κB/LCN-2 proteins and upregulating the gene expression of Insr/Irs-1/Glut4 in the insulin signaling pathway in the inflammatory environment.
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- 2022
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9. Research on the Regional Environmental Impact and Risk Assessment Affected by Mineral Resource Development: A Case Study of the Taojia River Watershed in Hunan
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Hongying Cao, Ziwei Wang, Xiaoyong Liao, You Li, and Yongbing Zhu
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mineral resource development ,heavy metals ,environmental quality ,risk assessment ,source analysis ,Evolution ,QH359-425 ,Ecology ,QH540-549.5 - Abstract
The mining and production of mineral resources can directly lead to soil and water pollution, posing a serious threat to human health. In this study, the Taojia River basin, a tributary of the Xiangjiang River, was selected as the study area. Based on the concentrations of heavy metals (As, Cd, Pb, and Zn) in 653 soil/substrate samples collected from 342 points in the study area in 2013 and 2021, the changes in soil heavy metal concentrations in the region were systematically analyzed to assess their environmental risks and impacts on regional environmental quality. The results showed that from 2013 to 2021, the As, Pb, and Zn pollution in regional soil, tailing sand, and surface water was reduced, while the Cd pollution increased. The average soil As, Pb, and Zn concentrations decreased from 3,750, 2,340, and 1,180 mg/kg to 457, 373, and 387mg/kg, respectively, while the Cd concentration increased from 0.11to 1.91 mg/kg; additionally, the overall distribution trend of heavy metal concentrations was high in the south, low in the north, and gradually decreased from upstream to downstream. The single evaluation index of heavy metal pollution risk showed that the percentages of medium to heavy pollution points in the soil As, Pb, and Zn were 84, 57, and 28%, respectively, in 2013, and this index decreased to 38, 37, and 25%, respectively, in 2021. The regional environmental quality was closely related to the intensity of mineral resource development. From 2000 to 2010, frequent mining development activities led to an increase in the area of construction land and a continuous decrease in the area of arable land, grassland, and vegetation cover in the region. During 2010–2020, the area of construction land decreased, and the vegetation coverage increased. The comprehensive evaluation index showed that the overall soil pollution risk in the watershed decreased, and the proportion of heavily polluted points decreased from 80 to 65%. It was shown by principal component analysis and factor analysis that mining development activities were the largest source of heavy metal pollution, in addition to mixed sources of tailings, solid waste, agriculture, and traffic emission sources. The results provide a scientific basis for the management and risk control of heavy metal pollution in the Taojia River basin.
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- 2022
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10. Therapeutic Effects of Berberine Hydrochloride on Stress-Induced Diarrhea-Predominant Irritable Bowel Syndrome Rats by Inhibiting Neurotransmission in Colonic Smooth Muscle
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Yulin Lu, Jingjing Huang, Yao Zhang, Zitong Huang, Weiming Yan, Tianran Zhou, Zhesheng Wang, Lu Liao, Hongying Cao, and Bo Tan
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berberine hydrochloride ,diarrhea-predominant irritable bowel syndrome ,colonic longitudinal smooth muscles ,inhibitory effects ,neurotransmission ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The etiology of diarrhea-predominant irritable bowel syndrome (IBS-D) is complicated and closely related to neurotransmission in the gastrointestinal (GI) tract. Developing new strategies for treating this disease is a major challenge for IBS-D research. Berberine hydrochloride (BBH), the derivative of berberine, is a herbal constituent used to treat IBS. Previous studies have shown that BBH has potential anti-inflammatory, antibacterial, analgesic, and antidiarrheal effects and a wide range of biological activities, especially in regulating the release of some neurotransmitters. A modified IBS-D rat model induced by chronic restraint stress was used in all experiments to study the effects of BBH on the GI tract. This study measured the abdominal withdrawal reflex (AWR) response to graded colorectal distention (CRD; 20, 40, 60, and 80 mmHg) and observed the fecal areas of stress-induced IBS-D model. Experiments were conducted using organ bath techniques, which were performed in vitro using strips of colonic longitudinal smooth muscle. Inhibitory and excitatory neurotransmitter agents were added to each organ bath to observe contractile responses on the strips and the treatment effect exerted by BBH. The IBS-D rat model was successfully induced by chronic restraint stress, which resulted in an increased defecation frequency and visceral hypersensitivity similar to that of humans. BBH could reduce 4-h fecal areas and AWR response to CRD in IBS-D. The stress-induced IBS-D model showed upregulated colonic mRNA expression levels of 5-hydroxytryptamine-3A receptor and downregulated expression levels of neuronal nitric oxide synthase. Meanwhile, BBH could reverse this outcome. The responses of substances that regulate the contraction induced by related neurotransmission in the longitudinal smooth muscle of IBS-D colon (including the agonist of acetylcholine, carbachol; NOS inhibitor, L-NAME; and P2Y1 receptor antagonist, MRS2500) can be inhibited by BBH. In summary, BBH promotes defecation frequency and visceral hypersensitivity in IBS-D and exerts inhibitory effects on contractile responses in colonic longitudinal smooth muscle. Thus, BBH may represent a new therapeutic approach for treating IBS-D.
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- 2021
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11. Unraveling the Novel Effect of Patchouli Alcohol Against the Antibiotic Resistance of Helicobacter pylori
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Yuanzun Zhong, Liyao Tang, Qiuhua Deng, Li Jing, Jiao Zhang, Yao Zhang, Feng Yu, Yijun Ou, Shaoju Guo, Bin Huang, Hongying Cao, Ping Huang, and Yifei Xu
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Helicobacter pylori ,patchouli alcohol ,resistance ,clarithromycin ,intracellular ,Microbiology ,QR1-502 - Abstract
The long-term colonization of Helicobacter pylori can cause various gastrointestinal diseases, and its high genetic variability is prone to antibiotic resistance and leads to failure of clinical treatment. Intracellular survival also contributes to the drug tolerance of H. pylori. Patchouli alcohol (PA) shows a highly efficient activity against H. pylori in vitro and in vivo. And this study aims to explore whether PA can reduce the resistance of H. pylori and determine the underlying mechanism. Checkerboard and time–kill bactericidal curve assay reveal that the combination of PA and clarithromycin (CLR) promoted the inhibition and bactericidal effect against H. pylori. Stimulation of CLR leads to the internalization of H. pylori, but PA can effectively inhibit the invasion induced by CLR. Compared with antibiotics, PA remarkably eradicated the intracellular H. pylori, and this intracellular sterilized ability was further improved in combination with antibiotics (CLR and metronidazole). The expression of H. pylori efflux pump genes (hp0605, hp1327, and hp1489) was dose-dependently downregulated by PA. Digital droplet PCR indicated that the H. pylori mutant of A2143G can be inhibited by PA. Cellular uptake and transport assays showed that PA is rapidly absorbed, which promotes its activity against intracellular bacteria. Therefore, PA can act synergistically with CLR as a candidate treatment against drug-resistant H. pylori.
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- 2021
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12. Clinical Strains of Helicobacter pylori With Strong Cell Invasiveness and the Protective Effect of Patchouli Alcohol by Improving miR-30b/C Mediated Xenophagy
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Yifei Xu, Qiuhua Deng, Yuanzun Zhong, Li Jing, Haiwen Li, Jingwei Li, Huimin Yu, Huafeng Pan, Shaoju Guo, Hongying Cao, Ping Huang, and Bin Huang
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helicobacer pylori ,patchouli alcohol ,xenophagy ,Intracellular ,drug resistance ,miR-30 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Helicobacter pylori was classified by the World Health Organization as a class 1 carcinogen. The development of drug-resistant strains of this pathogen poses a serious threat to human health worldwide. The cell invasion of H. pylori activates xenophagy in gastric epithelial cells by mediating miR-30b/c, and the emergence of autophagosomes provides a niche that enables the survival of intracellular H. pylori and promotes its drug resistance. This study revealed that some clinical drug-resistant H. pylori strains present much stronger invasive ability than standard strains. Patchouli alcohol (PA), a tricyclic sesquiterpene from Pogostemon cablin (Blanco) Benth (Labiatae), showed reliable activity against intracellular H. pylori. The mechanisms appeared to involve the downregulation of miR-30c-3p/5p and miR-30b-5p, thereby upregulating xenophagy-related gene expression (ULK1, ATG5, ATG12, and ATG14) and enhancing xenophagy. PA also inhibited the nuclear transfection of miR-30b-5p induced by H. pylori, thereby enhancing transcription factor EB function and increasing lysosome activity. The finding of strongly invasive intracellular H. pylori has great implications for clinical treatment, and PA can act against invasive H. pylori based on the improvement of miR-30b/c mediated xenophagy. Taken together, the results demonstrate that PA have potential use as a candidate medication for intracellular drug-resistant H. pylori.
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- 2021
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13. Effects of Radix Linderae extracts on a mouse model of diabetic bladder dysfunction in later decompensated phase
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Xufeng Yang, Dawei Lian, Pinglong Fan, Yifei Xu, Jing Wang, Fangjun Chen, Huanling Lai, Weiwen Jiang, Linjie Zhang, Ping Huang, and Hongying Cao
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Diabetic bladder dysfunction ,Later decompensated phase ,Radix Linderae ,High-fat diet ,Streptozotocin ,Other systems of medicine ,RZ201-999 - Abstract
Abstract Background This study aimed to elucidate the effects and mechanisms of Radix Linderae (RL) extracts on a mouse model of diabetic bladder dysfunction (DBD), especially on later decompensated phase. Methods Male C57BL/6J mice were intraperitoneally injected with streptozotocin (STZ) after 4 weeks of high-fat diet (HFD) feeding. DBD mouse models (later decompensated phase) were developed by 12-weeks persistent hyperglycemia and then treated with RL extracts for 4 weeks. During administration, the fasting blood glucose (FBG) test was performed once a week. Four weeks later, oral glucose tolerance test (OGTT), voided stain on paper (VSOP), and urodynamic alteration were explored. We also performed haematoxylin and eosin (H&E) and Masson’s trichrome staining to observe the histology of the bladder. Then, the contractile responses to α, β-methylene ATP, capsaicin (CAP), KCl and carbachol were measured. Moreover, qPCR assay was performed to analyse the bladder gene expression levels of M3 receptors and TRPV1. Results The diabetic mice exhibited higher FBG, OGTT and urine production, and no substantial alteration was observed after RL treatment. Urodynamic test showed the maximum bladder capacity (MBC), residual volume (RV) and bladder compliance (BC), as well as the decrement of voided efficiency (VE) and micturition volume (MV), remarkably increased in the DBD mice. Furthermore, RL treatment significant improved urodynamic urination, with lower MBC, RV, and, BC, as well as higher VE and MV, as compared with the model groups. The wall thickness of the bladder and the ratio of smooth muscle/collagen remarkably increased, and RL could effectively attenuate the pathological change. The response of bladder strips to the stimulus was also reduced in the DBD mice, and RL treatment markedly increased the contraction. Furthermore, the gene expression levels of M3 receptors and TRPV1 were down-regulated in the bladders of the diabetic mice, whereas RL treatment retrieved those gene expression levels. Conclusions RL extracts can improve the bladder voiding functions of the DBD model mice in later decompensated phase, and underlying mechanisms was associated with mediating the gene expression of M3 receptors and TRPV1 in the bladder instead of improving blood sugar levels.
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- 2019
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14. Diabetic bladder dysfunction in T2D KK-Ay mice and its changes in the level of relevant gene expression
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Jiao Zhang, Yao Zhang, Xufeng Yang, Jing Wang, Yifei Xu, Rui Wang, Bo Tan, Ping Huang, and Hongying Cao
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KK-Ay mice ,Type 2 diabetes ,Diabetic bladder dysfunction ,Nerve conduction ,Myosin Va ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Objective: Diabetic bladder dysfunction (DBD) is one of the most common and bothersome complications of diabetes mellitus (DM). The purpose of the present study is to investigate DBD in KK-Ay mice, and to identify the expression of relative genes. Method: Totally twenty-seven KK-Ay mice and thirty C57BL/6 J mice, respectively, were randomly divided into 12-, 18-, and 25-week old groups. The weight, water intake, voided volume, the frequency of micturition, fasting blood glucose (FBG), oral glucose tolerance test (OGTT) were measured at varying time points. Maximum bladder volume (MBC), residual volume (RV), bladder compliance (BC), micturition efficiency (VE) and maximum micturition pressure (MVP) were assessed by urodynamic test, and contractile responses to α, β‐methylene ATP, KCl, electrical‐field stimulation, carbachol were performed by detrusor smooth muscle strips contractility test. The bladders were stained with hematoxylin and eosin (H&E) and Masson’s trichrome to determine bladder wall thickness. Additionally, the mRNA expression of Myosin Va, SLC17A9, P2X1, M3 and M2 were then verified by qRT-PCR. Result: The weight, water intake, voided volumes, micturition frequency, FBG, the blood glucose AUC0-2h of KK-Ay mice were significantly increased at three time points. MBC, RV and BC were significantly increased; VE was significantly lower at the age of 18 and 25 weeks in KK-Ay mice; MVP was significantly increased at the age of 25 weeks in KK-Ay mice. In DSM strips contractility test, the amplitude of the spontaneous activity in KK-Ay mice significant increased at 12 weeks and 18 weeks, while both the amplitude and frequency were significantly decreased at the age of 25 weeks. The level of Myosin Va, SLC17A9 and M3 receptor significantly decreased in KK-Ay mice at 12 weeks, while Myosin Va markedly increased at 18 weeks; P2X1 and M2 receptors of KK-Ay mice was significantly increased at all three time points. Conclusion: Taken together, this study demonstrates that KK-Ay mice can be a proper model to investigate DBD whose transformation from compensatory state to decompensated state may ascribe to the time-dependent alternations of Myosin Va, SLC17A9, P2X1, M3 and M2 expression levels.
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- 2020
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15. Prognostic value of liver stiffness measurement for the liver-related surgical outcomes of patients under hepatic resection: A meta-analysis.
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Zitong Huang, Jingjing Huang, Tianran Zhou, Hongying Cao, and Bo Tan
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Medicine ,Science - Abstract
Previous studies have discussed the liver stiffness measurement (LSM) performance on predicting liver-related surgical outcomes for patients of hepatocellular carcinoma (HCC) under hepatic resection, yet there is much variation in reporting and consistency of findings. Therefore, we report a meta-analysis on this issue.We comprehensively searched PubMed, Embase, and Web of science to find the eligible cohort studies. The pooled Odds Ratios (OR) and 95% confidence intervals (CIs) were calculated to evaluate effect. The weighted mean LSM value was calculated as the optimal LSM cut-off value among studies.12 prospective cohort studies and one retrospective cohort study, including a total of 1942 cases were identified. The pooled results showed that preoperative LSM is significantly associated with the occurrence of overall postoperative complications (OR 1.76, 95% CI 1.46-2.11). In addition, a weighted mean LSM value of 14.2 kPa and 11.3KPa were suggested as the optimal LSM cut-off value reference using transient elastoqraphy (TE) for predicting overall postoperative complications in Asia countries and European countries, respectively.Preoperative LSM should be taken into account cautiously in the management of patients undergoing hepatectomy of HCC. Future studies could focus on setting a prognostic model integrated with LSM in predicting post-hepatectomy outcomes.
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- 2018
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16. Predictive value of combining urinary N-acetyl-β-Dglucosaminidase and serum homocysteine for contrastinduced nephropathy in patients after percutaneous coronary intervention.
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Yiling Zhai, Changjun Luo, Nianying Qin, Hongying Cao, Chunyang Dong, Zhou Huang, Dongling Huang, Fan Wang, Wanxia Wei, Jincheng Li, Jie Yang, Xueling Lu, Zhengzhuang Huang, and Wei Wang
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- 2024
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17. TRPV1 Dysfunction Impairs Gastric Nitrergic Neuromuscular Relaxation in High-Fat Diet–Induced Diabetic Gastroparesis Mice
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Siyuan Xu, Shaochan Liang, Ying Pei, Rui Wang, Yao Zhang, Yifei Xu, Bin Huang, Haiwen Li, Juanjuan Li, Bo Tan, Hongying Cao, and Shaoju Guo
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Pathology and Forensic Medicine - Published
- 2023
18. Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats.
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Wanyu Chen, Lu Liao, Zitong Huang, Yulin Lu, Yukang Lin, Ying Pei, Shulin Yi, Chen Huang, Hongying Cao, and Bo Tan
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IRRITABLE colon ,ENTERIC nervous system ,NEURAL transmission ,SUBMUCOUS plexus ,SUBSTANCE P ,GASTROINTESTINAL motility ,RATS - Abstract
Background and Purpose: Irritable bowel syndrome (IBS) is usually associated with chronic gastrointestinal disorders. Its most common subtype is accompanied with diarrhea (IBS-D). The enteric nervous system (ENS) modulates major gastrointestinal motility and functions whose aberration may induce IBS-D. The enteric neurons are susceptible to long-term neurotransmitter level alterations. The patchouli alcohol (PA), extracted from Pogostemonis Herba, has been reported to regulate neurotransmitter release in the ENS, while its effectiveness against IBS-D and the underlying mechanism remain unknown. Experimental Approach: In this study, we established an IBS-D model in rats through chronic restraint stress. We administered the rats with 5, 10, and 20 mg/kg of PA for intestinal and visceral examinations. The longitudinal muscle myenteric plexus (LMMP) neurons were further immunohistochemically stained for quantitative, morphological, and neurotransmitters analyses. Key Results: We found that PA decreased visceral sensitivity, diarrhea symptoms and intestinal transit in the IBS-D rats. Meanwhile, 10 and 20 mg/kg of PA significantly reduced the proportion of excitatory LMMP neurons in the distal colon, decreased the number of acetylcholine (Ach)- and substance P (SP)-positive neurons in the distal colon and restored the levels of Ach and SP in the IBS-D rats. Conclusion and Implications: These findings indicated that PA modulated LMMP excitatory neuron activities, improved intestinal motility and alleviated IBS-induced diarrheal symptoms, suggesting the potential therapeutic efficacy of PA against IBS-D. [ABSTRACT FROM AUTHOR]
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- 2024
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19. Effects of NBP on postoperative cognitive dysfunction in rats via Nrf 2/ARE pathway
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Jianshuai He, Junqiong Gao, He Zhu, Yang Zhao, Xiaotian Zhang, Xiufang Wang, Shengnan Wan, Hongying Cao, Lin Zhai, Yuanyong Wang, and Shilei Wang
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Aging ,Cell Biology - Published
- 2023
20. Three-dimensional delineation of soil pollutants at contaminated sites: Progress and prospects
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Huan Tao, Xiaoyong Liao, Hongying Cao, Dan Zhao, and Yixuan Hou
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Earth and Planetary Sciences (miscellaneous) - Published
- 2022
21. Excitatory Nerve Conduction Changed In a Partial BOO-induced OAB Rat Model
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Jingyi Huang, Hongliang Li, Yao Zhang, Jiaye Liu, Hongying Cao, and Yongling Long
- Abstract
Background Overactive bladder (OAB) is a common, long-term symptom complex with a high prevalence in women worldwide. The pathogenesis of OAB has not been elucidated. This study investigated the excitatory purinergic and cholinergic nerve conduction in OAB rat model induced by partial bladder outlet obstruction(PBOO). Methods Model rats underwent bladder outlet obstruction surgery. In the 2nd, 3rd, and 4th week after surgery, metabolic cages were used to detect the 12-hours Urine volume of the rats in the sham and model groups. The urodynamic parameters Bladder leak point pressure (BPLL), Maximum voiding pressure (MVP), Residual volume (RV), Maximum bladder capacity (MBC), Bladder compliance (BC), Voided efficiency (VE), and Non-voiding contractions (NVCs) were also detected. Besides, the time point above examined the contractile responses of isolated detrusor muscles to electrical and carbachol stimulation. 4th week after surgery, the bladders of both groups were taken for hematoxylin, eosin (H&E), and Masson’s trichrome staining. Besides, real-time qPCR and Western blot were performed to quantify the expression of Choline acetyltransferase(ChAT) and Solute carrier family 17 member 9 (SLC17A9). Results At week 4, compared with the sham group, the 12 h Urine volume increase significantly. The BLPP、MVP、VE、MBC、NVCs increased significantly, and the VE was significantly reduced. The contractile responses of isolated detrusor muscles to electrical and carbachol stimulation were significantly increased. In 4 week PBOO group, the bladder wall and the ratio of bladder muscle to collagen within the bladder smooth muscle layer wall were significantly higher than in the sham group. ChAT and SLC17A9 mRNA and protein expression in OAB model rats was significantly increased. Conclusions 4 weeks after PBOO, the OAB model was successfully established. The gene and protein expressions of ChAT and SLC17A9 were increased in the bladder of OAB model, suggesting that OAB may relate to the increased excitatory purinergic and cholinergic nerve conduction.
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- 2023
22. Patchouli Alcohol Improves Intestinal Motility in Ibs-D Rats Through Muscularis Macrophages Modulation of Enteric Neurons
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Ying Pei, Chen Huang, Wanyu Chen, Yao Zhang, Rui Bao, Shulin Yi, Ting Li, Yifei Xu, Hongying Cao, and Bo Tan
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- 2023
23. miR-30c Increases the Intracellular Survival of Helicobacter pylori by Inhibiting Autophagy
- Author
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Qiuhua Deng, Yifei Xu, Yuanzun Zhong, Liyao Tang, Si Du, Jiongming Yang, Lingping Wu, Shaoju Guo, Bin Huang, Hongying Cao, and Ping Huang
- Subjects
Article Subject ,Virology ,Immunology ,Microbiology - Abstract
Persistent Helicobacter pylori infection causes a variety of gastrointestinal diseases and even gastric cancer. H. pylori invades gastric epithelial cells to survive and proliferate, which is one of the key factors in persistent colonization. A Published study has confirmed that cells can eliminate intracellular H. pylori through xenophagy to maintain intracellular balance. However, a growing body of evidences indicate that H. pylori can inhibit xenophagy by miRNA through regulating the expression of key autophagy-related genes. Through western blot analysis, mRFP-GFP-LC3 transfection assay, and transmission electron microscopy, we found that H. pylori infection obstructed autophagy flux degradation stage in GES-1 cell lines. Gentamicin protection assay confirmed that inhibit xenophagy is benefit for intracellular H. pylori survive. miR-30c-1-3p and miR-30c-5p were upregulated in GES-1 cell lines after infecting with H. pylori, resulting in the negative regulation on xenophagy. Further studies through bioinformatics analysis and dual-luciferase reporter assays confirmed that ATG14 and ULK1 were the target genes of miR-30c-1-3p and that ATG12 was the target gene of miR-30c-5p. The overexpression of miR-30c-1-3p and miR-30c-5p reduces the expression of ATG14, ULK1, and ATG12 at mRNA level and also decreased intracellular H. pylori elimination in GES-1 cells. The above results suggested that the inhibition on xenophagy by miR-30c-1-3p and miR-30c-5p through ATG14, ULK1, and ATG12 targeting benefitted intracellular H. pylori in the evasion of xenophagy clearance.
- Published
- 2022
24. Variations of microbiota in three types of typical military contaminated sites: Diversities, structures, influence factors, and co-occurrence patterns
- Author
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Junpeng Luo, You Li, Hongying Cao, Yongbing Zhu, Xiaodong Liu, Haonan Li, and Xiaoyong Liao
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Environmental Engineering ,Triazines ,Health, Toxicology and Mutagenesis ,Microbiota ,Pollution ,Azocines ,Soil ,Military Personnel ,Biodegradation, Environmental ,Environmental Chemistry ,Humans ,Soil Pollutants ,Waste Management and Disposal ,Trinitrotoluene - Abstract
Contamination with energetic compounds (ECs) is common in military sites and poses a great risk to the environment and human health. However, its effects on the soil bacterial communities remain unclear. This study assessed the variations of bacterial communities, co-occurrence patterns, and their influence factors in three types of typical military-contaminated sites (artillery range, military-industrial site, and ammunition destruction site). The results showed that the most polluted sites were ammunition destruction sites, followed by military-industrial sites, whereas pollution in the artillery ranges was minimal. The average concentrations of ECs including 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) in the study sites ranged 120-1.67 × 10
- Published
- 2022
25. Planning analysis on metropolitan marginal area of Beijing along with its expansion.
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Yongfu Li 0005, Anrong Dang, and Hongying Cao
- Published
- 2010
- Full Text
- View/download PDF
26. Conservation oriented accessibility assessment for historical and cultural towns: Taking Zhouzhuang town as an example.
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Yongfu Li 0005, Anrong Dang, and Hongying Cao
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- 2010
- Full Text
- View/download PDF
27. Spatial-vertical variations of energetic compounds and microbial community response in soils from an ammunition demolition site in China
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Huijun Zhang, Yongbing Zhu, Shiyu Wang, Sanping Zhao, Yaguang Nie, Chao Ji, Qing Wang, Xiaoyong Liao, Hongying Cao, and Xiaodong Liu
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Environmental Engineering ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Published
- 2023
28. Three-dimensional distribution characteristics of multiple pollutants in the soil at a steelworks mega-site based on multi-source information
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Yixuan Hou, You Li, Huan Tao, Hongying Cao, Xiaoyong Liao, and Xiaodong Liu
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Environmental Engineering ,Health, Toxicology and Mutagenesis ,Environmental Chemistry ,Pollution ,Waste Management and Disposal - Published
- 2023
29. Patchouli alcohol improved diarrhea-predominant irritable bowel syndrome by regulating excitatory neurotransmission in the myenteric plexus of rats
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Bo Tan, wanyu chen, lu liao, zitong huang, yulin lu, yukang lin, ying pei, shulin yi, chen huang, and hongying cao
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Pharmacology ,Pharmacology (medical) - Abstract
Background and Purpose: Irritable bowel syndrome (IBS) is usually associated with chronic gastrointestinal disorders. Its most common subtype is accompanied with diarrhea (IBS-D). The enteric nervous system (ENS) modulates major gastrointestinal motility and functions whose aberration may induce IBS-D. The enteric neurons are susceptible to long-term neurotransmitter level alterations. The patchouli alcohol (PA), extracted from Pogostemonis Herba, has been reported to regulate neurotransmitter release in the ENS, while its effectiveness against IBS-D and the underlying mechanism remain unknown.Experimental Approach: In this study, we established an IBS-D model in rats through chronic restraint stress. We administered the rats with 5, 10, and 20 mg/kg of PA for intestinal and visceral examinations. The longitudinal muscle myenteric plexus (LMMP) neurons were further immunohistochemically stained for quantitative, morphological, and neurotransmitters analyses.Key Results: We found that PA decreased visceral sensitivity, diarrhea symptoms and intestinal transit in the IBS-D rats. Meanwhile, 10 and 20 mg/kg of PA significantly reduced the proportion of excitatory LMMP neurons in the distal colon, decreased the number of acetylcholine (Ach)- and substance P (SP)-positive neurons in the distal colon and restored the levels of Ach and SP in the IBS-D rats.Conclusion and Implications: These findings indicated that PA modulated LMMP excitatory neuron activities, improved intestinal motility and alleviated IBS-induced diarrheal symptoms, suggesting the potential therapeutic efficacy of PA against IBS-D.
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- 2022
30. An improved non-stationary geostatistical method for three-dimensional interpolation of Benzo(a)pyrene at a contaminated site
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You Li, Yixuan Hou, Huan Tao, Hongying Cao, Xiaodong Liu, Ziwei Wang, and Xiaoyong Liao
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Soil ,Spatial Analysis ,Environmental Engineering ,Benzo(a)pyrene ,Environmental Chemistry ,Soil Pollutants ,Pollution ,Waste Management and Disposal ,Environmental Monitoring - Abstract
Intense industrial activities and complex hydrogeological conditions at contaminated sites make accurate three-dimensional (3D) mapping challenging. The cause is the non-stationarity in the variance of soil pollutants in geographical space (G-space), making the stationary hypothesis required by the Kriging method unsatisfactory. To handle the variance non-stationarity, a Variance-Octree-Kriging (VOK) method was proposed. VOK is a spatial deformation method that constructs a stationary deformation space (D-space) by stretching and shrinking the G-spaces with low and high spatial correlation, respectively. VOK method consists of 3D stratification in G-space, space scaling and transformation, and ordinary Kriging (OK) in D-space. 3D stratification uses variance octree (VOT) to generate a set of anchor points in the G-space. The spatial scaling and transformation use the virtual force algorithm (VFA) and thin-plate spline to evenly distribute the anchor points and obtain the D-space, where the OK is implemented. The method was applied to predict the distribution of soil Benzo(a)pyrene (BaP) at a contaminated site in North China Plain. The results show that the interpolation accuracy of VOK was 9% higher than that of OK. The VOK method also changed the spatial structure from anisotropic to isotropic. The root mean squared error (RMSE) of fill, silt and clay layers decreased by 4.67%, 11.39%, and 20.46%, respectively. This method is applicable to the 3D interpolation of pollutants at contaminated sites, with the advantages of high interpolation accuracy and the ability to handle the non-stationarity in variance.
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- 2022
31. Relaxation Effect of Patchouli Alcohol in Rat Corpus Cavernous and Its Underlying Mechanisms
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Hongying Cao, Jing Wang, Ping Huang, Xufeng Yang, Yifei Xu, and Fang-jun Chen
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Article Subject ,Endothelium ,Sildenafil ,chemistry.chemical_element ,Calcium ,Pharmacology ,Glibenclamide ,Other systems of medicine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Enos ,medicine ,030304 developmental biology ,0303 health sciences ,Tetraethylammonium ,biology ,Potassium channel blocker ,biology.organism_classification ,Nitric oxide synthase ,medicine.anatomical_structure ,Complementary and alternative medicine ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,RZ201-999 ,Research Article ,medicine.drug - Abstract
In this study, we investigated the relaxation effect and mechanisms of patchouli alcohol (PA) on rat corpus cavernosum. Corpus cavernosum strips were used in organ baths for isometric tension studies. The results showed that PA demonstrated concentration-dependent relaxation effect on rat corpus cavernosum. The relaxant response to PA was not influenced by tetrodotoxin and atropine while it was significantly inhibited by removal of endothelium. L-NG-nitroarginine methyl ester (L-NAME, a nitric oxide synthase inhibitor) or 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, a soluble guanylate cyclase inhibitor) significantly inhibited relaxation response to PA, whereas indomethacin (COX inhibitor) had no effect on PA-induced relaxation. The treatment of endothelium-deprived corpus cavernosum with several potassium channel blockers including tetraethylammonium (TEA), 4-aminopyridine (4-AP), and glibenclamide had no effect on PA-induced relaxation. Endothelium-deprived corpus cavernosal contractions induced by cumulative addition of Ca2+ to high KCl solution without CaCl2 were significantly inhibited by PA. Also, PA improved relaxant capacity of sildenafil in rat corpus cavernosum. In addition, the perfusion with PA significantly increased the levels of cGMP and expression of mRNA and protein of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS). Furthermore, intracavernous injection of PA enhanced the rise in intracavernous pressure in rats during cavernosal nerve electric stimulation. In conclusion, PA relaxed the rat corpus cavernosum attributed to both endothelium-dependent and -independent properties. While the former component was mostly involved in nitric oxide signaling pathway, the endothelium-independent mechanism involved in PA-induced relaxation was probably linked to calcium antagonism.
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- 2020
32. Suo Quan Wan ameliorates bladder overactivity and regulates neurotransmission via regulating Myosin Va protein expression
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Yao Zhang, Jiao Zhang, Ming Hong, Jingyi Huang, Siyuan Xu, Rui Wang, Na Zhou, Ping Huang, Bo Tan, and Hongying Cao
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Pharmacology ,Urinary Bladder ,Pharmaceutical Science ,Myosins ,Synaptic Transmission ,Streptozocin ,Diabetes Mellitus, Experimental ,Rats ,Urodynamics ,Complementary and alternative medicine ,Drug Discovery ,Molecular Medicine ,Animals ,Muscle Contraction - Abstract
Ancient prescriptions of Suo Quan Wan (SQW) have therapeutic effects on diabetic bladder dysfunction. However, the underlying mechanism remains unclear. Here, we hypothesized that SQW ameliorates bladder overactivity and regulates neurotransmission via regulating Myosin Va protein expression.After diabetic rats were induced by streptozotocin (65 mg/kg), the model of diabetic bladder dysfunction was established by detecting fasting blood glucose, urodynamic test, in vitro muscle strip experiments, and histological examination. One week after induction, SQW was given to observe the therapeutic effect. The expression levels of Myosin Va in control, Model, SQW L and SQW H groups were detected by RT-qPCR, RNAscope and immunofluorescence assay. The expression levels of ChAT, SP, nNOS and VIP proteins were observed by immunofluorescence assay. After knockdown and overexpression of Myosin Va, the expression changes of ChAT, SP, nNOS and VIP and the regulatory role of SQW were observed.STZ-induced DM rats had significantly higher serum glucose levels and lower body weight. Compared with the diabetic rats, SQW treatment significantly improved urination function with decreased residual volume (RV), bladder compliance (BC), non-voiding contractions (NVCs), and increased voided efficiency (VE). In addition, contractile responses of muscle strips to electrical-field stimulation (EFS), carbachol (CCh), KCl were significantly lower in the SQW H and SQW L groups than those in the model group. RT-qPCR found that the expression of Myosin Va in the bladder tissue or bladder neurons in model group was significantly increased compared with the control group, and SQW treatment significantly decreased the levels of Myosin Va. In DM rats, ChAT and SP expression were significantly increased, while nNOS and VIP expression were significantly decreased, and SQW improved this phenomenon. Interestingly, SQW ameliorated the abnormal expression of ChAT, SP, nNOS and VIP caused by myosin Va knockdown, and Myosin Va overexpression results are consistent with these.SQW ameliorates overactive bladder and regulate neurotransmission via regulating Myosin Va mRNA and protein expression.
- Published
- 2021
33. New perspective on human health risk from polycyclic aromatic hydrocarbons (PAHs) on surfaces of structures and buildings for industrial legacy before and after remediation
- Author
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Hongying Cao, Bayier Wulijia, Lin Wang, You Li, and Xiaoyong Liao
- Subjects
Renewable Energy, Sustainability and the Environment ,Strategy and Management ,Building and Construction ,Industrial and Manufacturing Engineering ,General Environmental Science - Published
- 2022
34. Therapeutic Effects of Berberine Hydrochloride on Stress-Induced Diarrhea-Predominant Irritable Bowel Syndrome Rats by Inhibiting Neurotransmission in Colonic Smooth Muscle
- Author
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Hongying Cao, Lu Liao, Tianran Zhou, Yulin Lu, Zi-Tong Huang, Yao Zhang, Bo Tan, Weiming Yan, Jing-Jing Huang, and Zhesheng Wang
- Subjects
Agonist ,Carbachol ,medicine.drug_class ,RM1-950 ,Pharmacology ,Neurotransmission ,colonic longitudinal smooth muscles ,chemistry.chemical_compound ,Berberine ,medicine ,Pharmacology (medical) ,neurotransmission ,Receptor ,Irritable bowel syndrome ,Original Research ,business.industry ,inhibitory effects ,Antagonist ,diarrhea-predominant irritable bowel syndrome ,medicine.disease ,berberine hydrochloride ,chemistry ,Therapeutics. Pharmacology ,business ,Acetylcholine ,medicine.drug - Abstract
The etiology of diarrhea-predominant irritable bowel syndrome (IBS-D) is complicated and closely related to neurotransmission in the gastrointestinal (GI) tract. Developing new strategies for treating this disease is a major challenge for IBS-D research. Berberine hydrochloride (BBH), the derivative of berberine, is a herbal constituent used to treat IBS. Previous studies have shown that BBH has potential anti-inflammatory, antibacterial, analgesic, and antidiarrheal effects and a wide range of biological activities, especially in regulating the release of some neurotransmitters. A modified IBS-D rat model induced by chronic restraint stress was used in all experiments to study the effects of BBH on the GI tract. This study measured the abdominal withdrawal reflex (AWR) response to graded colorectal distention (CRD; 20, 40, 60, and 80 mmHg) and observed the fecal areas of stress-induced IBS-D model. Experiments were conducted using organ bath techniques, which were performed in vitro using strips of colonic longitudinal smooth muscle. Inhibitory and excitatory neurotransmitter agents were added to each organ bath to observe contractile responses on the strips and the treatment effect exerted by BBH. The IBS-D rat model was successfully induced by chronic restraint stress, which resulted in an increased defecation frequency and visceral hypersensitivity similar to that of humans. BBH could reduce 4-h fecal areas and AWR response to CRD in IBS-D. The stress-induced IBS-D model showed upregulated colonic mRNA expression levels of 5-hydroxytryptamine-3A receptor and downregulated expression levels of neuronal nitric oxide synthase. Meanwhile, BBH could reverse this outcome. The responses of substances that regulate the contraction induced by related neurotransmission in the longitudinal smooth muscle of IBS-D colon (including the agonist of acetylcholine, carbachol; NOS inhibitor, L-NAME; and P2Y1 receptor antagonist, MRS2500) can be inhibited by BBH. In summary, BBH promotes defecation frequency and visceral hypersensitivity in IBS-D and exerts inhibitory effects on contractile responses in colonic longitudinal smooth muscle. Thus, BBH may represent a new therapeutic approach for treating IBS-D.
- Published
- 2021
35. Time‐dependent functional, morphological, and molecular changes in diabetic bladder dysfunction in streptozotocin‐induced diabetic mice
- Author
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Li-jun Fu, Fang-jun Chen, Yifei Xu, Hongying Cao, Ping Huang, Jing Wang, Rui‐Wang, Wen-Kang Ren, Xufeng Yang, Li-yao Tang, and Bo Tan
- Subjects
Male ,medicine.medical_specialty ,Carbachol ,Urology ,Myosin Type V ,Drinking ,Original Basic Science Articles ,030232 urology & nephrology ,Stimulation ,compensated state ,temporal changes ,Diabetes Mellitus, Experimental ,Mice ,decompensated state ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,Myosin ,medicine ,Animals ,RNA, Messenger ,Messenger RNA ,030219 obstetrics & reproductive medicine ,Myosin Heavy Chains ,medicine.diagnostic_test ,business.industry ,Body Weight ,Original Basic Science Article ,Urinary Bladder Diseases ,Cystometry ,Diabetic mouse ,Glucose Tolerance Test ,Streptozotocin ,medicine.disease ,Electric Stimulation ,Stimulation, Chemical ,Mice, Inbred C57BL ,Urodynamics ,SLC17A9 ,diabetic bladder dysfunction (DBD) ,Endocrinology ,Nucleotide Transport Proteins ,Neurology (clinical) ,business ,myosin Va ,Muscle Contraction ,medicine.drug - Abstract
Aim Diabetic bladder dysfunction (DBD) is one of the most common and bothersome complications of diabetes mellitus (DM). This study aimed to investigate the functional, structural, and molecular changes of the bladder at 0, 3, 6, 9, and 12 weeks after DM induction by streptozotocin (STZ) in male C57BL/6 mice. Methods Male C57BL/6J mice were injected with STZ (130 mg/kg). Then, diabetic general characteristics, cystometry test, histomorphometry, and contractile responses to α, β‐methylene ATP, KCl, electrical‐field stimulation, carbachol were performed at 0, 3, 6, 9, and 12 weeks after induction. Finally, protein and messenger RNA (mRNA) expressions of myosin Va and SLC17A9 were quantified. Results DM mice exhibited lower body weight, voiding efficiency and higher water intake, urine production, fasting blood glucose, oral glucose tolerance test, bladder wall thickness, maximum bladder capacity, residual volume, bladder compliance. In particular, nonvoiding contractions has increased more than five times at 6 weeks. And the amplitudes of spontaneous activity, contractile responses to all stimulus was about two times higher at 6 weeks but cut almost in half at 12 weeks. The protein and mRNA expressions of myosin Va and SLC17A9 were about two times higher at 6 weeks, but myosin Va was reverted nearly 40% while SLC17A9 is still higher at 12 weeks. Conclusions DBD transitioned from a compensated state to a decompensated state in STZ‐induced DM mice at 9 to 12 weeks after DM induction. Our molecular data suggest that the transition may be closely related to the alterations of myosin Va and SLC17A9 expression levels in the bladder with time.
- Published
- 2019
36. Removal of polycyclic aromatic hydrocarbons from different soil fractions by persulfate oxidation
- Author
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Hongying Cao, Xiaoyong Liao, You Li, Qiongzhi Liu, and Xuegang Gong
- Subjects
Environmental Engineering ,Environmental remediation ,0211 other engineering and technologies ,Fraction (chemistry) ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Soil ,polycyclic compounds ,Soil Pollutants ,Environmental Chemistry ,Humic acid ,Organic matter ,Polycyclic Aromatic Hydrocarbons ,Environmental Restoration and Remediation ,0105 earth and related environmental sciences ,General Environmental Science ,chemistry.chemical_classification ,021110 strategic, defence & security studies ,Sulfates ,General Medicine ,Persulfate ,Soil contamination ,chemistry ,Environmental chemistry ,Composition (visual arts) ,Oxidation process ,Oxidation-Reduction - Abstract
Removal of polycyclic aromatic hydrocarbons (PAHs) from different soil fractions of contaminated soil was investigated by using activated persulfate oxidation remediation in our research. The results showed that the light fraction, which accounted for only 10% of the soil, contained 30% of the PAHs at a concentration of 4352 mg/kg. The heavy fraction contained more high-molecular-weight PAHs, and the total PAH concentration was 625 mg/kg. After being oxidized, the removal rate of PAHs was 39% in the light fraction and nearly 90% in the heavy fraction. Among the different fractions of the heavy fraction, humic acid contained the highest concentration of PAHs, and consequently, the highest removal efficiency of PAHs was also in humic acid. Compared with the light fraction, the heavy fraction has more aromatic compounds and those compounds were broken down during the oxidation process, which may be the removal mechanism involved in the oxidation of high-ring PAHs. Similarly, the enhancement of C=C bonds after oxidation can also explain the poor removal of high-ring PAHs in the light fraction. These results imply that different fractions of soil vary in composition and structure, leading to differences in the distribution and oxidation efficiencies of PAHs.
- Published
- 2019
37. Codelivery of GRP78 siRNA and docetaxel via RGD-PEG-DSPE/DOPA/CaP nanoparticles for the treatment of castration-resistant prostate cancer
- Author
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Xiangyu Zhang, Liang Li, Haiyan Zhang, Zelai He, Fanzhong Lin, Longquan Xiang, and Hongying Cao
- Subjects
0301 basic medicine ,Pharmacology ,Drug ,Small interfering RNA ,Chemistry ,media_common.quotation_subject ,Pharmaceutical Science ,Cancer ,Gene delivery ,medicine.disease ,03 medical and health sciences ,Prostate cancer ,030104 developmental biology ,0302 clinical medicine ,Docetaxel ,In vivo ,030220 oncology & carcinogenesis ,Drug Discovery ,Drug delivery ,medicine ,Cancer research ,medicine.drug ,media_common - Abstract
Background: Castration-resistant prostate cancer (CRPC) accounts for the majority of prostate cancer deaths, and patients with CRPC are prone to developing drug resistance. Therefore, there is a need to develop effective therapeutics to treat CRPC, especially drug-resistant CRPC. Although various nanoparticles have been developed for drug or gene delivery and control release, approaches to reproducibly formulate the optimal treatment with nanoparticles that could effectively target CRPC and bone metastasis remain suboptimal. Recently, codelivery of a chemotherapeutic agent and a small interfering RNA (siRNA) has become a promising strategy for the treatment of drug-resistant prostate cancer. Methods: In a previous study, we prepared a novel RGD-PEG-DSPE/CaP nanoparticle as an effective and biocompatible drug and gene delivery system. In this study, we further modify the nanoparticle to obtain the LCP-RGD nanoparticle, which contains a calcium phosphate (CaP) core, dioleoyl phosphatidic acid (DOPA) and RGD modified poly(ethylene glycol)-conjugated distearoyl phosphatidylethanolamine (RGD-PEG-DSPE). This drug delivery system was used for codelivery of GRP78 siRNA and docetaxel (DTXL) for the treatment of the PC-3 CRPC. Results: The nanoparticles contain the CaP core, which can effectively compress the negatively charged siRNA, while the DOPA and RGD-PEG-DSPE component can effectively carry DTXL. The arginine-glycine-aspartic acid (RGD) segment can target the prostate cancer site, as the cancer site is neovascularized. This novel nanoparticle has good stability, excellent biocompatibility, high drug and siRNA loading capacity, and an in vitro sustainable release profile. Conclusion: Codelivery of DTXL and GRP78 siRNA has enhanced in vitro and in vivo anti-prostate cancer effects which are much greater than using free DTXL and free GRP78 siRNA together. Our study also indicated that codelivery of DTXL and GRP78 siRNA have an in vitro and in vivo combinational anti-prostate cancer effect and also could effectively sensitize the cell-killing effect of DTXL; this method may be especially suitable for drug-resistant CRPC treatment.
- Published
- 2019
38. Sensitive detection of polycyclic aromatic hydrocarbons with gold colloid coupled chloride ion SERS sensor
- Author
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Xiaoyong Liao, Hongying Cao, Xuegang Gong, Yishu Zhao, You Li, Haonan Li, and Daniel P. Cassidy
- Subjects
02 engineering and technology ,01 natural sciences ,Biochemistry ,Chloride ,Analytical Chemistry ,chemistry.chemical_compound ,symbols.namesake ,Electrochemistry ,medicine ,Environmental Chemistry ,Spectroscopy ,Naphthalene ,Trisodium citrate ,Detection limit ,010401 analytical chemistry ,Substrate (chemistry) ,Phenanthrene ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,chemistry ,symbols ,Pyrene ,0210 nano-technology ,Raman spectroscopy ,medicine.drug ,Nuclear chemistry - Abstract
A simple surface-enhanced Raman spectroscopy (SERS) sensor based on an undecorated gold-colloid substrate was developed for the rapid and effective detection of polycyclic aromatic hydrocarbons (PAH). The SERS enhancement of the bare Au nanoparticles for PAH was achieved by adjusting chemical reduction conditions and Cl- content. The strongest SERS response of this system was achieved with 2.0 mL of trisodium citrate (1%) and 80 μL of NaCl (1 M). With this simple SERS sensor, qualitative and quantitative determination of trace-level naphthalene (NaP), phenanthrene (PHE) and pyrene (PYR) were achieved using a portable Raman spectrometer at detection limits of 1.38 μg L-1, 0.23 μg L-1, and 0.45 μg L-1, respectively. Plots of SERS intensity vs. PAH concentrations were linear, with correlation coefficients (R2) ranging from 0.8729 to 0.9994. More importantly, the SERS sensor was able to accurately identify each PAH in complex mixtures. This SERS technique shows great promise for the rapid and direct detection of aromatic hydrocarbons organic pollutants in field.
- Published
- 2019
39. Clinical Strains of
- Author
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Yifei, Xu, Qiuhua, Deng, Yuanzun, Zhong, Li, Jing, Haiwen, Li, Jingwei, Li, Huimin, Yu, Huafeng, Pan, Shaoju, Guo, Hongying, Cao, Ping, Huang, and Bin, Huang
- Subjects
Pharmacology ,drug resistance ,xenophagy ,helicobacer pylori ,miR-30 ,Intracellular ,Original Research ,patchouli alcohol - Abstract
Helicobacter pylori was classified by the World Health Organization as a class 1 carcinogen. The development of drug-resistant strains of this pathogen poses a serious threat to human health worldwide. The cell invasion of H. pylori activates xenophagy in gastric epithelial cells by mediating miR-30b/c, and the emergence of autophagosomes provides a niche that enables the survival of intracellular H. pylori and promotes its drug resistance. This study revealed that some clinical drug-resistant H. pylori strains present much stronger invasive ability than standard strains. Patchouli alcohol (PA), a tricyclic sesquiterpene from Pogostemon cablin (Blanco) Benth (Labiatae), showed reliable activity against intracellular H. pylori. The mechanisms appeared to involve the downregulation of miR-30c-3p/5p and miR-30b-5p, thereby upregulating xenophagy-related gene expression (ULK1, ATG5, ATG12, and ATG14) and enhancing xenophagy. PA also inhibited the nuclear transfection of miR-30b-5p induced by H. pylori, thereby enhancing transcription factor EB function and increasing lysosome activity. The finding of strongly invasive intracellular H. pylori has great implications for clinical treatment, and PA can act against invasive H. pylori based on the improvement of miR-30b/c mediated xenophagy. Taken together, the results demonstrate that PA have potential use as a candidate medication for intracellular drug-resistant H. pylori.
- Published
- 2021
40. Diabetic bladder dysfunction in T2D KK-Ay mice and its changes in the level of relevant gene expression
- Author
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Jing Wang, Ping Huang, Bo Tan, Yao Zhang, Xufeng Yang, Rui Wang, Jiao Zhang, Hongying Cao, and Yifei Xu
- Subjects
0301 basic medicine ,Blood Glucose ,Male ,medicine.medical_specialty ,Carbachol ,Nerve conduction ,media_common.quotation_subject ,Myosin Type V ,Urinary Bladder ,H&E stain ,Stimulation ,Type 2 diabetes ,RM1-950 ,Urination ,Streptozocin ,Diabetes Mellitus, Experimental ,Myosin Va ,Contractility ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,Myosin ,medicine ,Animals ,RNA, Messenger ,media_common ,Pharmacology ,Myosin Heavy Chains ,business.industry ,KK-Ay mice ,Diabetic bladder dysfunction ,General Medicine ,medicine.disease ,Mice, Inbred C57BL ,Receptors, Purinergic P2X1 ,Urodynamics ,030104 developmental biology ,Endocrinology ,Diabetes Mellitus, Type 2 ,030220 oncology & carcinogenesis ,Nucleotide Transport Proteins ,Therapeutics. Pharmacology ,business ,medicine.drug ,Muscle Contraction - Abstract
Objective: Diabetic bladder dysfunction (DBD) is one of the most common and bothersome complications of diabetes mellitus (DM). The purpose of the present study is to investigate DBD in KK-Ay mice, and to identify the expression of relative genes. Method: Totally twenty-seven KK-Ay mice and thirty C57BL/6 J mice, respectively, were randomly divided into 12-, 18-, and 25-week old groups. The weight, water intake, voided volume, the frequency of micturition, fasting blood glucose (FBG), oral glucose tolerance test (OGTT) were measured at varying time points. Maximum bladder volume (MBC), residual volume (RV), bladder compliance (BC), micturition efficiency (VE) and maximum micturition pressure (MVP) were assessed by urodynamic test, and contractile responses to α, β‐methylene ATP, KCl, electrical‐field stimulation, carbachol were performed by detrusor smooth muscle strips contractility test. The bladders were stained with hematoxylin and eosin (H&E) and Masson’s trichrome to determine bladder wall thickness. Additionally, the mRNA expression of Myosin Va, SLC17A9, P2X1, M3 and M2 were then verified by qRT-PCR. Result: The weight, water intake, voided volumes, micturition frequency, FBG, the blood glucose AUC0-2h of KK-Ay mice were significantly increased at three time points. MBC, RV and BC were significantly increased; VE was significantly lower at the age of 18 and 25 weeks in KK-Ay mice; MVP was significantly increased at the age of 25 weeks in KK-Ay mice. In DSM strips contractility test, the amplitude of the spontaneous activity in KK-Ay mice significant increased at 12 weeks and 18 weeks, while both the amplitude and frequency were significantly decreased at the age of 25 weeks. The level of Myosin Va, SLC17A9 and M3 receptor significantly decreased in KK-Ay mice at 12 weeks, while Myosin Va markedly increased at 18 weeks; P2X1 and M2 receptors of KK-Ay mice was significantly increased at all three time points. Conclusion: Taken together, this study demonstrates that KK-Ay mice can be a proper model to investigate DBD whose transformation from compensatory state to decompensated state may ascribe to the time-dependent alternations of Myosin Va, SLC17A9, P2X1, M3 and M2 expression levels.
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- 2020
41. Study on optimization and stability of a single dose streptozotocin-induced diabetic modeling in rats
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Yao Zhang, jiao Zhang, Ming Hong, Jingyi Huang, Rui Wang, Bo Tan, Ping Huang, and Hongying Cao
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endocrine system diseases ,nutritional and metabolic diseases - Abstract
BackgroundOptimization of experimental conditions in streptozotocin induced diabetic model in Sprague Dawley (SD) rats to evaluate the stability of the model.MethodsMale and female SD rats were randomly divided into control group, STZ 45 group (STZ: 45 mg / kg), STZ 65 group (STZ: 65 mg / kg), STZ 85 group (STZ: 85 mg / kg), high fat diet with STZ 45 group (STZ: 45 mg / kg), high fat diet with STZ 65 group (STZ: 65 mg / kg), high fat diet with STZ 85 group (STZ: 85 mg / kg). N = 6 in each group. The changes of body weight and blood glucose were observed dynamically.ResultsThere was no significant difference in blood glucose or body weight between the STZ 45 group and the control group in both male and female rats, whether or not they were on a high-fat diet. However, there were significant differences in blood glucose between the high-dose STZ group and the control group in both male and female rats, regardless of whether the rats were on a high-fat diet or not (P < 0.05 or P < 0.01). Compared with the control group, there were significant differences in blood glucose levels (P < 0.05 or P < 0.01) and higher blood glucose levels in the male rats fed with the normal diet than that in those fed with the high-fat diet.ConclusionsIn this study, male rats fed with ordinary feed and injected STZ dose of 65 mg / kg were the most stable and ideal diabetic rat.
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- 2020
42. Contamination characteristics of energetic compounds in soils of two different types of military demolition range in China
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Huijun Zhang, Yongbing Zhu, Shiyu Wang, Sanping Zhao, Yaguang Nie, Xiaoyong Liao, Hongying Cao, Hao Yin, and Xiaodong Liu
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Soil ,Military Personnel ,Explosive Agents ,Triazines ,Health, Toxicology and Mutagenesis ,Humans ,Soil Pollutants ,General Medicine ,Toxicology ,Pollution ,Trinitrotoluene - Abstract
The pollution of energetic compounds (ECs) in military ranges has become the focus of worldwide attention. However, few studies on the contamination of ECs at Chinese military ranges have been reported to date. In this study, two different types of military demolition range in China, Dunhua (DH) and Taiyuan (TY), were investigated and the ECs in their soils were determined. 10 ECs were detected at both ranges. While all the contamination characteristics were distinct, 2,4,6-trinitrotoluene (TNT) was the most abundant contamination source in soils at DH range, with an average concentration of 1106 mg kg
- Published
- 2022
43. Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
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Zi-Tong Huang, Bo Tan, Yao Zhang, Rui Wang, Wen-Kang Ren, Jiao Zhang, Ping Huang, and Hongying Cao
- Subjects
Nervous system ,Pathology ,medicine.medical_specialty ,media_common.quotation_subject ,Neuroregulation ,Urinary system ,General Chemical Engineering ,Immunocytochemistry ,Urinary Bladder ,Cell Culture Techniques ,Cell Separation ,Biology ,Immunofluorescence ,Urination ,Synaptic vesicle ,General Biochemistry, Genetics and Molecular Biology ,Rats, Sprague-Dawley ,medicine ,Animals ,media_common ,Neurons ,medicine.diagnostic_test ,General Immunology and Microbiology ,General Neuroscience ,Choline acetyltransferase ,Rats ,medicine.anatomical_structure ,nervous system ,Neuroglia - Abstract
The lower urinary tract has two main functions, namely, periodic urine storage and micturition; these functions are mediated through central and peripheral neuroregulation. Although extensive research on the lower urinary tract nervous system has been conducted, most studies have focused on primary culture. This protocol introduces a method for the isolation and culture of bladder neurons and glia from Sprague-Dawley rats. In this method, the neurons and glia were incubated in a 37 °C, 5% CO2 incubator for 5-7 days. As a result, they grew into mature shapes suitable for related subsequent immunofluorescence experiments. Cells were morphologically observed using an optical microscope. Neurons, synaptic vesicles, and glia were identified by β-III-tubulin and MAP-2, Synapsin-1, and GFAP staining, respectively. Meanwhile, immunocytochemistry was performed on several neurotransmitter-related proteins, such as choline acetyltransferase, DYNLL2, and SLC17A9.
- Published
- 2020
44. RETRACTED: Knockdown of lncRNA CCAT2 inhibits endometrial cancer cells growth and metastasis via sponging miR-216b
- Author
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Pengmu Xie, Hongying Cao, Ying Li, Jianhua Wang, and Zhumei Cui
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,Apoptosis ,Biology ,Metastasis ,Phosphatidylinositol 3-Kinases ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,Internal medicine ,Genetics ,medicine ,Humans ,PTEN ,Neoplasm Metastasis ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Oncogene ,Cell growth ,Endometrial cancer ,PTEN Phosphohydrolase ,General Medicine ,Transfection ,Middle Aged ,medicine.disease ,Endometrial Neoplasms ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,Proto-Oncogene Proteins c-bcl-2 ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Female ,RNA Interference ,RNA, Long Noncoding ,Proto-Oncogene Proteins c-akt ,Signal Transduction - Abstract
Objective Colon cancer-associated transcript 2 (CCAT2), a novel lncRNA has been reported as an oncogene in several cancers. This study was aimed to explore whether CCAT2 also exerted oncogenic roles in endometrial cancer cells. Materials and methods The expression of CCAT2 in 30 pairs of endometrial cancer and matched non-cancerous tissues were detected by qRT-PCR. Two endometrial cancer cell lines HEC-1-A and RL95-2 were used throughout this study. CCAT2 in cells was silenced by transfection with shRNA targeted CCAT2, then cell growth and metastasis were assessed by performing trypan blue staining, Transwell assay, and flow cytometry. Dual-luciferase reporter assay was used to detect the combination of miR-216b and CCAT2. Besides, the expression of miR-216b and Bcl-2 in cells were overexpressed or suppressed by transfection with their correspondingly mimic/vector or inhibitor/shRNA. qRT-PCR and western blot analysis were performed to detect the expression of Bcl-2 and main factors in PTEN/PI3K/AKT and mTOR signaling pathways. Results CCAT2 was highly expressed in endometrial cancer tissues when compared to non-cancerous endometrial tissues. Knockdown of CCAT2 inhibited HEC-1-A and RL95-2 cells viability, migration, invasion, but induced apoptosis. CCAT2 was an endogenous sponge by competing for miR-216b, and miR-216b suppression alleviated CCAT2 silence-diminished cell growth and metastasis. miR-216b negatively regulated Bcl-2 and Bcl-2 could further active PTEN/PI3K/AKT and mTOR signaling pathways. Conclusions To conclude, these results demonstrated lncRNA CCAT2 was highly expressed in endometrial cancer tissues. Knockdown of CCAT2 inhibited cell growth and metastasis of endometrial cancer cells by sponging miR-216b.
- Published
- 2017
45. Resonant Frequency Evaluation on Reradiation Interference from Power Transmission Line Based on the Generalized Resonance
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Liu Ren, Jiang Haotian, Tang Bo, Sun Rui, and Hongying Cao
- Subjects
Physics ,Frequency band ,020209 energy ,Resonance ,Zero-point energy ,02 engineering and technology ,Condensed Matter Physics ,Interference (wave propagation) ,Electronic, Optical and Magnetic Materials ,Computational physics ,Electric power transmission ,Nuclear magnetic resonance ,Poynting's theorem ,Reradiation ,0202 electrical engineering, electronic engineering, information engineering ,Electrical and Electronic Engineering ,Antenna (radio) - Abstract
IEEE was not able to give the resonance mechanism of reradiation interference (RRI) above 1.7 MHz from power lines based on research works of electric field. In order to solve this problem, the generalized resonance theory is introduced, and a method based on the electromagnetic (EM) energy balance theory is proposed to predict the RRI resonant frequency. Taking a large-scale space including UHV power lines and antenna arrays as a generalized closed system, the expression of EM open systems' generalized resonance factor is derived based on the complex Poynting theorem. Then the RRI resonant frequency corresponds to the zero point of the generalized resonance factor's imaginary part. Taking the case researched by IEEE as an example, the maximum deviation of the resonant frequency between the values predicted by the generalized resonance theory and the measurement values obtained from scaled model experiments are less than ±0.169 MHz in the frequency band below 1.7 MHz.
- Published
- 2016
46. Additional file 2: of Effects of Radix Linderae extracts on a mouse model of diabetic bladder dysfunction in later decompensated phase
- Author
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Xufeng Yang, Dawei Lian, Pinglong Fan, Yifei Xu, Wang, Jing, Fangjun Chen, Huanling Lai, Weiwen Jiang, Linjie Zhang, Huang, Ping, and Hongying Cao
- Abstract
Original recordings of the detrusor strips contraction to stimulus. (DOCX 595 kb)
- Published
- 2019
- Full Text
- View/download PDF
47. Codelivery of GRP78 siRNA and docetaxel via RGD-PEG-DSPE/DOPA/CaP nanoparticles for the treatment of castration-resistant prostate cancer
- Author
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Xiangyu, Zhang, Zelai, He, Longquan, Xiang, Liang, Li, Haiyan, Zhang, Fanzhong, Lin, and Hongying, Cao
- Subjects
Calcium Phosphates ,Male ,Cell Survival ,Mice, Nude ,Antineoplastic Agents ,Docetaxel ,codelivery ,RANK ,Polyethylene Glycols ,Mice ,Drug Delivery Systems ,Animals ,Humans ,RNA, Small Interfering ,Endoplasmic Reticulum Chaperone BiP ,Heat-Shock Proteins ,Cell Proliferation ,Original Research ,Mice, Inbred BALB C ,Phosphatidylethanolamines ,Dihydroxyphenylalanine ,Prostatic Neoplasms, Castration-Resistant ,siRNA ,PC-3 Cells ,Nanoparticles ,Drug Screening Assays, Antitumor ,Oligopeptides - Abstract
Background: Castration-resistant prostate cancer (CRPC) accounts for the majority of prostate cancer deaths, and patients with CRPC are prone to developing drug resistance. Therefore, there is a need to develop effective therapeutics to treat CRPC, especially drug-resistant CRPC. Although various nanoparticles have been developed for drug or gene delivery and control release, approaches to reproducibly formulate the optimal treatment with nanoparticles that could effectively target CRPC and bone metastasis remain suboptimal. Recently, codelivery of a chemotherapeutic agent and a small interfering RNA (siRNA) has become a promising strategy for the treatment of drug-resistant prostate cancer. Methods: In a previous study, we prepared a novel RGD-PEG-DSPE/CaP nanoparticle as an effective and biocompatible drug and gene delivery system. In this study, we further modify the nanoparticle to obtain the LCP-RGD nanoparticle, which contains a calcium phosphate (CaP) core, dioleoyl phosphatidic acid (DOPA) and RGD modified poly(ethylene glycol)-conjugated distearoyl phosphatidylethanolamine (RGD-PEG-DSPE). This drug delivery system was used for codelivery of GRP78 siRNA and docetaxel (DTXL) for the treatment of the PC-3 CRPC. Results: The nanoparticles contain the CaP core, which can effectively compress the negatively charged siRNA, while the DOPA and RGD-PEG-DSPE component can effectively carry DTXL. The arginine-glycine-aspartic acid (RGD) segment can target the prostate cancer site, as the cancer site is neovascularized. This novel nanoparticle has good stability, excellent biocompatibility, high drug and siRNA loading capacity, and an in vitro sustainable release profile. Conclusion: Codelivery of DTXL and GRP78 siRNA has enhanced in vitro and in vivo anti-prostate cancer effects which are much greater than using free DTXL and free GRP78 siRNA together. Our study also indicated that codelivery of DTXL and GRP78 siRNA have an in vitro and in vivo combinational anti-prostate cancer effect and also could effectively sensitize the cell-killing effect of DTXL; this method may be especially suitable for drug-resistant CRPC treatment.
- Published
- 2018
48. Effect of various chemical oxidation reagents on soil indigenous microbial diversity in remediation of soil contaminated by PAHs
- Author
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Chunming Su, Hongying Cao, Xiaoyong Liao, You Li, and Zeying Wu
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inorganic chemicals ,Environmental Engineering ,Environmental remediation ,Health, Toxicology and Mutagenesis ,Iron ,0208 environmental biotechnology ,Population ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Article ,chemistry.chemical_compound ,Soil ,Bioremediation ,Potassium Permanganate ,Pseudomonas ,Environmental Chemistry ,Soil Pollutants ,Polycyclic Aromatic Hydrocarbons ,education ,Coke ,0105 earth and related environmental sciences ,Total organic carbon ,education.field_of_study ,Permanganate ,Public Health, Environmental and Occupational Health ,Oxides ,General Medicine ,General Chemistry ,Hydrogen Peroxide ,Persulfate ,Oxidants ,Pollution ,020801 environmental engineering ,Potassium permanganate ,Biodegradation, Environmental ,chemistry ,Manganese Compounds ,Reagent ,Environmental chemistry ,Oxidation-Reduction - Abstract
Chemical oxidation is a promising pretreatment step coupled with bioremediation for removal of polycyclic aromatic hydrocarbons (PAHs). The effectiveness of Fenton, modified Fenton, potassium permanganate and activated persulfate oxidation treatments on the real contaminated soils collected from a coal gas plant (263.6 ± 73.3 mg kg−1 of the Σ16 PAHs) and a coking plant (385.2 ± 39.6 mg kg−1 of the Σ16 PAHs) were evaluated. Microbial analyses showed only a slight impact on indigenous microbial diversity by Fenton treatment, but showed the inhibition of microbial diversity and delayed population recovery by potassium permanganate reagent. After potassium permanganate treatment, the microorganism mainly existed in the soil was Pseudomonas or Pseudomonadaceae. The results showed that total organic carbon (TOC) content in soil was significantly increased by adding modified Fenton reagent (1.4%–2.3%), while decreased by adding potassium permanganate (0.2%–1%), owing to the nonspecific and different oxidative properties of chemical oxidant. The results also demonstrated that the removal efficiency of total PAHs was ordered: permanganate (90.0%–92.4%) > activated persulfate (81.5%–86.54%) > modified Fenton (81.5%–85.4%) > Fenton (54.1%–60.0%). Furthermore, the PAHs removal efficiency was slightly increased on the 7th day after Fenton and modified Fenton treatments, about 14.6%, and 14.4% respectively, and the PAHs removal efficiency only enhanced 4.1% and 1.3% respectively from 1st to 15th day after potassium permanganate and activated persulfate treatments. The oxidants greatly affect the growth of soil indigenous microbes, which cause further influence for PAHs degradation by bioremediation.
- Published
- 2018
49. Quantification of Oxidant Demand and Consumption for In Situ Chemical Oxidation Design: in the Case of Potassium Permanganate
- Author
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Hongying Cao, Xiaoyong Liao, You Li, Daniel P. Cassidy, and Kun Yang
- Subjects
chemistry.chemical_classification ,Environmental Engineering ,Coefficient of determination ,Chemistry ,Ecological Modeling ,Potassium ,0208 environmental biotechnology ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Pollution ,020801 environmental engineering ,Reaction rate ,Potassium permanganate ,chemistry.chemical_compound ,In situ chemical oxidation ,Loam ,Environmental chemistry ,Soil water ,Environmental Chemistry ,Organic matter ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
Accurate estimation of oxidant consumption during in situ chemical oxidation (ISCO) is the key to determining the treatment effectiveness in contaminated sites. We established the estimation model of soil oxidant demand (SOD) and simulation equations of potassium permanganate (KMnO4) dynamic consumption based on the reaction equation of KMnO4 with reductive minerals and the estimation model of SOD. Model validation, model application, and simulation assessment had been accomplished. Results indicated that the simulations are in good agreement with measured data. The confidence level of the SOD estimation model of KMnO4 was over 80%, with sensitivity in decreasing order as follows: organic matter content > initial KMnO4 concentration > reductive minerals (RMs). Particularly, the organic matter played a dominate role in the SOD model estimation. The coefficient of determination (R2) of the SOD dynamic consumption simulation equation was above 0.9. Among the various types of soils, the overall trend of SOD value and reaction period decreased as follows: clay > loam > sand. However, the consumption rate of KMnO4 decreased in the order of clay > sand > loam. In addition, SOD value, reaction period, and reaction rate all increased as the initial concentration of KMnO4 went up. This work can provide a methodology and reference for selecting and estimating of the optimal oxidant doses and reaction period during field application.
- Published
- 2018
50. Effect of patchouli alcohol on Helicobacter pylori-induced neutrophil recruitment and activation
- Author
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Wen-Hui Wei, Yuan-Hui Deng, Yifei Xu, Li-jun Fu, Jing Wang, Da-wei Lian, Fang-jun Chen, Xufeng Yang, Ping Huang, Wen-Kang Ren, and Hongying Cao
- Subjects
0301 basic medicine ,Male ,Neutrophils ,Immunology ,Anti-Inflammatory Agents ,Pharmacology ,Helicobacter Infections ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,In vivo ,Gene expression ,Gastric mucosa ,medicine ,Immunology and Allergy ,Animals ,Adhesins, Bacterial ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Acute Gastritis ,Helicobacter pylori ,NADPH Oxidases ,Gene Expression Regulation, Bacterial ,medicine.disease ,biology.organism_classification ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Neutrophil Infiltration ,030220 oncology & carcinogenesis ,Cytokines ,Reactive Oxygen Species ,Infiltration (medical) ,Sesquiterpenes - Abstract
Neutrophil infiltration typically occurs in Helicobacter pylori (H. pylori)-induced acute gastritis; however, this immune response fails to eradicate H. pylori in vivo. Moreover, reactive oxygen species (ROS), which are generated by neutrophils, cause severe damage to gastric mucosa. Patchouli alcohol (PA) has been reported to have effective anti-oxidative and anti-H. pylori activities, and we investigated its effects on H. pylori-induced neutrophil recruitment and activation in this research. In neutrophil recruitment experiment, H. pylori was injected into rat air pouch to explore the effects of PA (10, 20 and 40 mg/kg) on acute inflammatory response. The results revealed that PA significantly reduced the weight of exudate and the number of neutrophils in the air pouch. Meanwhile, remarkable decrements in TNF-α and IL-8 levels in exudates were observed. In neutrophil activation experiment, rat neutrophils were isolated and activated by using 50 μg/mL H. pylori water-soluble surface protein with or without the treatment of PA (5, 10 or 20 μmol/L). Results indicated that PA not only significantly inhibited the production of ROS, but also reduced the gene and protein expressions of p22/p47-phoxes, and the binding of p22/p47-phoxes. Furthermore, the influence of PA on the neutrophil activation genes of H. pylori (h-nap and sabA) was investigated, and the results showed that expressions of h-nap and sabA were remarkably decreased after PA treatment. In conclusion, PA reduced the recruitment and activation of neutrophils induced by H. pylori, as shown by its inhibition of pro-inflammatory factor generation, p22/p47-phoxes function and H. pylori neutrophil activation-related gene expression.
- Published
- 2018
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