465 results on '"Hongling Li"'
Search Results
2. Artemether ameliorates acetaminophen-induced liver injury through Nrf2 pathway
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Sijie Yu, Na Yang, Hongling Li, Xiaodan Hu, Li Zhang, and Shibo Li
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Artemether ,Acetaminophen ,Liver injury ,Nrf2 ,Antioxidative stress ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Acetaminophen (APAP) overdose is a prevalent cause of clinical pharmacological liver injury worldwide. Artemether (ART), a first-line antimalarial drug, has demonstrated hepatoprotective activity. However, its effect on APAP-induced acute liver injury (AILI) remains unclear. In this study, we investigated whether ART can protect against AILI and examined its underlying mechanisms. In vivo, ART mitigated APAP-induced liver histological changes, including mitochondrial damage, hepatocyte necrosis, hepatocyte apoptosis, and inflammatory infiltration. Additionally, ART reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in APAP-induced mice. ART also activated the Nrf2-HO-1/GPX4 signaling pathway, exerting antioxidant effects in both in vitro and in vivo models of AILI. To confirm Nrf2 as a target of ART in vivo, we pretreated C57BL/6 mice with the Nrf2 inhibitor, ML385. The results indicated that inhibiting Nrf2 diminishes the protective effect of ART against AILI. Overall, our findings suggest that ART's protective effect against AILI is mediated through the Nrf2-related antioxidant pathway.
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- 2024
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3. Synthesis and characterization on anti-clay polycarboxylate superplasticizer in concrete
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Hongling Li, Yaqian Wang, Xinyu Yang, and Guowen Sun
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Anti-clay polycarboxylatesuperplasticizer ,Montmorillonite ,Cement mortar ,Factorial factorial design ,Response surface design ,Anti-clay ability ,Materials of engineering and construction. Mechanics of materials ,TA401-492 - Abstract
To address the issues of low anti-clay performance and high adsorption of ordinary superplasticizer in concrete applications, a novel anti-clay polycarboxylate superplasticizer (Abbreviated as KN-G superplasticizer) was developed. Its structure and properties were characterized using techniques such as infrared spectroscopy (IR), nuclear magnetic resonance (NMR), and X-ray diffraction pattern (XRD). The results indicate that the optimal mass ratio for the synthesized KN-G superplasticizer was monomer: sulfonic acid base: initiator quality: chain transfer agent: hydroxypropyl acrylate=0.808:0.129:0.008:0.008: 0.008:0.007:0.048 under specific conditions, including an acid-to-ether molar ratio of 4.0:1, temperature of 35 °C, solid content of 56%, and drop rate of 4 s/drop. The successful grafting of -SO3H functional groups was confirmed by test results of IR and NMR. At a water-to-cement ratio of 0.45, the disage of montmorillonite and KN-G superplasticizer at 3% and 0.5% of the cement mass, respectively, resulted in the fluidity of mortar with low-quality sand reaching 120 mm, demonstrating the effective anti-clay ability of the KN-G superplasticizer.
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- 2024
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4. Research on improved YOLOv8n based potato seedling detection in UAV remote sensing images
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Lining Wang, Guanping Wang, Sen Yang, Yan Liu, Xiaoping Yang, Bin Feng, Wei Sun, and Hongling Li
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potato seedling detection ,UAV remote sensing ,YOLOv8n ,lightweight ,VanillaNet ,GSConv ,Plant culture ,SB1-1110 - Abstract
IntroductionAccurate detection of potato seedlings is crucial for obtaining information on potato seedlings and ultimately increasing potato yield. This study aims to enhance the detection of potato seedlings in drone-captured images through a novel lightweight model.MethodsWe established a dataset of drone-captured images of potato seedlings and proposed the VBGS-YOLOv8n model, an improved version of YOLOv8n. This model employs a lighter VanillaNet as the backbone network in-stead of the original YOLOv8n model. To address the small target features of potato seedlings, we introduced a weighted bidirectional feature pyramid network to replace the path aggregation network, reducing information loss between network layers, facilitating rapid multi-scale feature fusion, and enhancing detection performance. Additionally, we incorporated GSConv and Slim-neck designs at the Neck section to balance accuracy while reducing model complexity. ResultsThe VBGS-YOLOv8n model, with 1,524,943 parameters and 4.2 billion FLOPs, achieves a precision of 97.1%, a mean average precision of 98.4%, and an inference time of 2.0ms. Comparative tests reveal that VBGS-YOLOv8n strikes a balance between detection accuracy, speed, and model efficiency compared to YOLOv8 and other mainstream networks. Specifically, compared to YOLOv8, the model parameters and FLOPs are reduced by 51.7% and 52.8% respectively, while precision and a mean average precision are improved by 1.4% and 0.8% respectively, and the inference time is reduced by 31.0%.DiscussionComparative tests with mainstream models, including YOLOv7, YOLOv5, RetinaNet, and QueryDet, demonstrate that VBGS-YOLOv8n outperforms these models in terms of detection accuracy, speed, and efficiency. The research highlights the effectiveness of VBGS-YOLOv8n in the efficient detection of potato seedlings in drone remote sensing images, providing a valuable reference for subsequent identification and deployment on mobile devices.
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- 2024
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5. Performance Study of a Chain–Spoon Seed Potato Discharger Based on DEM-MBD Coupling
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Wei Xing, Hua Zhang, Wei Sun, Hui Li, Xiaolong Liu, Hongling Li, Yangzhou Chen, and Yonggang Lu
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potato ,spoon–chain-type planter ,seed clearing ,DEM-MBD coupling ,Agriculture (General) ,S1-972 - Abstract
To address the issues of the poor filling and clearing efficiency of spoon–chain potato seed dischargers, an optimization design was implemented in this study. Based on the motion characteristics of potatoes during the filling and transport processes, an inclination angle was set for the seed spoon cavity, and a seed-clearing brush was installed at the top of the seed discharger. A DEM-MBD coupled simulation model of the seed discharger was constructed. The working speed of the driving sprocket, the inclination angle of the seed spoon cavity, and the seed holding height were used as experimental factors, while the single-seed qualification rate, missed seed rate, and over-seeding rate were used as evaluation indices to conduct a quadratic regression orthogonal rotation combination experiment. This determined the optimal technical parameter combination for the best working performance. Based on the results of the DEM-MBD coupled simulation experiments, a response surface optimization test was conducted. The results showed that the optimal working performance was achieved when the working speed of the driving sprocket was 43 rpm, the inclination angle of the seed spoon cavity was 15°, and the seed holding height was 0.2 m. Under these conditions, the single-seed qualification rate was 95.28%, the missed seed rate was 0.92%, and the over-seeding rate was 3.80%. Further soil bin tests confirmed that, under the optimal working parameters, the relative deviations of all test indices from the response surface optimization test results were less than 2%. The research results provide new insights for the optimization design of spoon–chain potato seed-metering devices.
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- 2024
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6. Investigation of the Traveling Performance of the Tracked Chassis of a Potato Combine Harvester in Hilly and Mountainous Areas
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Yangzhou Chen, Zeyu Wang, Hua Zhang, Xiaolong Liu, Hui Li, Wei Sun, and Hongling Li
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potato ,self-propelled combine harvester ,tracked undercarriage ,passing performance ,Agriculture (General) ,S1-972 - Abstract
Aiming at the problems of poor passability of a tracked chassis due to small plots, complicated road conditions and steep slopes during mechanized potato harvesting in hilly and mountainous areas. To design a potato combine harvester and take the tracked chassis of the harvester as the research object to study its driving performance under typical road conditions in mountainous areas. Firstly, mechanical analysis and theoretical calculation are carried out on the tracked chassis to obtain the relevant parameters of key components, and secondly, its driving performance is analyzed to obtain the driving limit values of passing performance under different working conditions; RecurDyn software was used to establish the dynamics simulation and analysis model of the whole machine, and the driving limit values of the harvester were determined under five different road conditions through simulation. The results show the following: the driving limit gradient angle is 20° in cross-gradient conditions, 25° in longitudinal up-gradient conditions, 25° in longitudinal down-gradient conditions, the limiting height of the chassis that can be overpassed in obstacle-crossing conditions is 450 mm, and the limiting width of the chassis that can be spanned in trench-crossing conditions is 1200 mm. The simulation results were verified through field tests, and the results of the field tests showed that the harvester met the requirements of stable travelling on longitudinal slopes of 24°, climbed over a 450 mm straight wall and crossed over a 1200 mm trench, which were similar to the simulation results, indicating that the simulation results were accurate and feasible, and met the design requirements for the travelling passage of the crawler potato harvester. This study provides an in-depth understanding of the tracked chassis of the potato combine harvester in hilly mountainous areas, with a view to providing reference for the design of tracked harvesters for other crops in hilly mountainous areas.
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- 2024
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7. Anlotinib in Chinese patients aged ≥70 years with advanced non-squamous non-small cell lung cancer without prior chemotherapy: a multicenter, single-arm pilot trial
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Da Zhao, Zhengguo Li, Xinli Hou, Lei Yang, Zeng Li, Li Yan, Hongling Li, Hua Liu, Xiaoping Liu, Feixue Song, Guixiang Li, Yu Zhang, and Xiaoming Hou
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non-squamous ,non-small-cell lung carcinoma ,anlotinib ,elderly ,pilot trial ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundBased on pharmacoeconomics, drug availability and actual treatment, optimal treatment regimens for Chinese non-small-cell lung carcinoma (NSCLC) patients over 70 years old are needed.MethodsThis multicenter, single-arm pilot trial enrolled patients with advanced non-squamous NSCLC who refused systemic chemotherapy. Eligible patients received anlotinib (12 mg/day, d1-14, Q3W) until disease progression, intolerant toxicities, or withdrawal from the study. The primary endpoint was progression-free survival (PFS).ResultsForty-nine patients were screened between January 2019 and September 2021, of whom 40 patients were eligible. The median age was 76 years. With a median follow-up period of 16.20 (95% CI: 8.77, 25.10) months, the median PFS was 5.45 months (95% CI: 3.52-9.23) and the median overall survival was 10.32 months (95% CI: 6.44-12.78). Three patients achieved a partial response and 34 had stable disease, with an objective response rate of 7.5% and a disease control rate of 92.5%. Thirty-three (82.5%; 33/40) patients reported treatment-related adverse events (TRAEs) of any grade, and the incidence rate of grade ≥3 TRAEs was 35% (14/40). The most common grade ≥3 TRAEs were hypertension (4/40; 10.0%), hand-foot syndrome (3/40; 7.5%), and proteinuria (2/40; 5.0%).ConclusionAnlotinib treatment was feasible and safe in Chinese elderly patients with advanced non-squamous NSCLC who did not receive any systemic chemotherapy.
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- 2024
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8. Lactate Protects Intestinal Epithelial Barrier Function from Dextran Sulfate Sodium-Induced Damage by GPR81 Signaling
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Xiaojing Li, Zhijie Yao, Jin Qian, Hongling Li, and Haitao Li
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colitis ,GPR81 ,lactate ,intestinal epithelial barrier ,MMP9 ,NF-κB ,Nutrition. Foods and food supply ,TX341-641 - Abstract
The dysregulation of the intestinal epithelial barrier significantly contributes to the inflammatory progression of ulcerative colitis. Recent studies have indicated that lactate, produced by gut bacteria or derived from fermented foods, plays a key role in modulating inflammation via G-protein-coupled receptor 81 (GPR81). In this study, we aimed to investigate the potential role of GPR81 in the progression of colitis and to assess the impact of lactate/GPR81 signaling on intestinal epithelial barrier function. Our findings demonstrated a downregulation of GPR81 protein expression in patients with colitis. Functional verification experiments showed that Gpr81-deficient mice exhibited more severe damage to the intestinal epithelial barrier and increased susceptibility to DSS-induced colitis, characterized by exacerbated oxidative stress, elevated inflammatory cytokine secretion, and impaired expression of tight-junction proteins. Mechanistically, we found that lactate could suppress TNF-α-induced MMP-9 expression and prevent the disruption of tight-junction proteins by inhibiting NF-κB activation through GPR81 in vitro. Furthermore, our study showed that dietary lactate could preserve intestinal epithelial barrier function against DSS-induced damage in a GPR81-dependent manner in vivo. Collectively, these results underscore the crucial involvement of the lactate/GPR81 signaling pathway in maintaining intestinal epithelial barrier function, providing a potential therapeutic strategy for ulcerative colitis.
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- 2024
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9. Real-world study of the effectiveness of BBIBP-CorV (Sinopharm) COVID-19 vaccine in the Kingdom of Morocco
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Yaowen Zhang, Jihane Belayachi, Yunkai Yang, Qiang Fu, Lance Rodewald, Hongling Li, Bing Yan, Ying Wang, Yanna Shen, Qian Yang, Weiyun Mu, Rong Tang, Chen Su, Tianfang Xu, Majdouline Obtel, Abdelkader Mhayi, Rachid Razine, Redouane Abouqal, Yuntao Zhang, and Xiaoming Yang
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COVID-19 ,Vaccine ,Effectiveness ,BBIBP-CorV ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The Kingdom of Morocco approved BBIBP-CorV (Sinopharm) COVID-19 vaccine for emergency use on 22 January 2021 in a two-dose, three-to-four-week interval schedule. We conducted a retrospective cohort study to determine real-world BBIBP-CorV vaccine effectiveness (VE) against serious or critical hospitalization of individuals RT-PCR-positive for SARS-CoV-2 during the first five months of BBIBP-CorV use in Morocco. Methods The study was conducted among adults 18–99 years old who were tested by RT-PCR for SARS-CoV-2 infection between 1 February and 30 June 2021. RT-PCR results were individually linked with outcomes from the COVID-19 severe or critical hospitalization dataset and with vaccination histories from the national vaccination registration system. Individuals with partial vaccination (
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- 2022
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10. Expression of GABPA and HO-1 in classical Hodgkin's lymphoma and their clinical significance
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Hongling Li, Li Xue, Dezhuan Da, and Xuhui Zhao
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Surgery ,RD1-811 - Published
- 2023
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11. Effect of transcranial direct current stimulation combined with a smart hand joint training device on hand dysfunction in patients with early stroke
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Long Zhao, Zibo Liu, Qingfeng Sun, and Hongling Li
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stroke ,hand function ,smart hand joint training device ,transcranial direct current stimulation ,Medicine - Published
- 2022
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12. Thinking and exploration of the process-oriented diagnosis and management mode for emergency consciousness disorder
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Hongling Li, Yanpeng Li, Jiaqian Ma, and Jianguo Li
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Surgery ,RD1-811 - Published
- 2023
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13. VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, induces cell cycle arrest and apoptosis in breast cancer cells by inhibiting the Wnt/β-catenin pathway
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Hongling Li, Chunyan Dang, Xiaohui Tai, Li Xue, Yuna Meng, Shuping Ma, and Jing Zhang
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Medicine ,Science - Abstract
Abstract Schiff base compounds and their metal complexes have become important synthetic organic drugs due to their extensive biological activities, which include anticancer, antibacterial and antiviral effects. In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced by VALD-3. Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. Western blot analysis revealed that VALD-3 upregulated pro-apoptotic proteins (Bad and Bax), downregulated anti-apoptotic proteins (Bcl-2, Bcl-xl, survivin and XIAP) and increased the expression of cleaved caspase-3, cleaved caspase-8, Cyto-c and cleaved PARP. VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. In addition, survival analysis showed that VALD-3 can significantly prolong the survival time of mice (P = 0.036). This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer.
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- 2021
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14. Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway
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Yanlei Yang, Junfen Fan, Haoying Xu, Linyuan Fan, Luchan Deng, Jing Li, Di Li, Hongling Li, Fengchun Zhang, and Robert Chunhua Zhao
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Long noncoding RNAs are crucial factors for modulating adipogenic differentiation, but only a few have been identified in humans. In the current study, we identified a previously unknown human long noncoding RNA, LYPLAL1-antisense RNA1 (LYPLAL1-AS1), which was dramatically upregulated during the adipogenic differentiation of human adipose-derived mesenchymal stem cells (hAMSCs). Based on 5′ and 3′ rapid amplification of cDNA ends assays, full-length LYPLAL1-AS1 was 523 nt. Knockdown of LYPLAL1-AS1 decreased the adipogenic differentiation of hAMSCs, whereas overexpression of LYPLAL1-AS1 enhanced this process. Desmoplakin (DSP) was identified as a direct target of LYPLAL1-AS1. Knockdown of DSP enhanced adipogenic differentiation and rescued the LYPLAL1-AS1 depletion-induced defect in adipogenic differentiation of hAMSCs. Further experiments showed that LYPLAL1-AS1 modulated DSP protein stability possibly via proteasome degradation, and the Wnt/β-catenin pathway was inhibited during adipogenic differentiation regulated by the LYPLAL1-AS1/DSP complex. Together, our work provides a new mechanism by which long noncoding RNA regulates adipogenic differentiation of human MSCs and suggests that LYPLAL1-AS1 may serve as a novel therapeutic target for preventing and combating diseases related to abnormal adipogenesis, such as obesity.
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- 2021
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15. Cross-Linked Versus Conventional Polyethylene for Long-Term Clinical Outcomes After Total Hip Arthroplasty: A Systematic Review and Meta-Analysis
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Jiangyuan Shi, Weicong Zhu, Shaohua Liang, Hongling Li, and Siming Li
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cross-linked ,hip arthroplasty ,polyethylene ,osteolysis ,revision ,radiological wear ,Surgery ,RD1-811 - Abstract
Background: Cross-linked polyethylene (HXLPE) liners have been used for total hip arthroplasty (THA) to address the problem of osteolysis and revision surgery associated with conventional polyethylene (CPE) liners. This systematic review and meta-analysis investigated the long-term efficacy of HXLPE in preventing revision surgery and radiological osteolysis in comparison to CPE. Materials and Methods: A comprehensive search of PubMed, Embase, and the Cochrane Library from their respective inception to September 2018 was conducted to identify potential candidate articles. Data were pooled using Stata software 14.0. The quality of randomized controlled trials (RCTs) and observational studies was assessed by two different authors using the Cochrane risk-of-bias tool and the Newcastle–Ottawa scale (NOS), respectively. Results: Eight RCTs and six observational studies were included in this review. The pooled results significantly favored HXLPE over CPE in terms of total number of revisions and radiological osteolysis, with a risk reduction of 78% (95% confidence interval [CI] 0.13–0.36; p
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- 2021
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16. Lnc13728 facilitates human mesenchymal stem cell adipogenic differentiation via positive regulation of ZBED3 and downregulation of the WNT/β-catenin pathway
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Haoying Xu, Yanlei Yang, Linyuan Fan, Luchan Deng, Junfen Fan, Di Li, Hongling Li, and Robert Chunhua Zhao
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Long noncoding RNA 13728 ,Adipogenic differentiation ,hADSCs ,ZBED3 ,WNT/β-catenin signal pathway ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Obesity has received increasing attention because of its widespread worldwide occurrence and many threats to health. Human adipose-derived mesenchymal stem cells (hADSCs) are a critical source of adipocytes. Long noncoding RNAs (lncRNAs) play pivotal roles in cell fate determination and differentiation. The objective of the present study was to identify and investigate the function and regulatory mechanism of lncRNAs on adipogenic differentiation of hADSCs. Methods We used lncRNA arrays to identify the prominent differentially expressed lncRNAs before and after hADSC adipogenic differentiation and verified their biological function through antisense oligonucleotide knockdown or lentivirus overexpression. The adipogenic differentiation of hADSCs was assessed by oil red O staining as well as the mRNA and protein levels of adipogenic marker genes through qRT-PCR and western blot. Bioinformatic tool LncPro and immunofluorescence was performed to uncover the interaction between lnc13728 and ZBED3. WNT/β-catenin signaling pathway was evaluated by western blot and immunofluorescence. Results The lncRNA arrays showed that lnc13728 expression was significantly upregulated after hADSC adipogenic differentiation and was correlated positively with the expression of the adipogenesis-related genes in human adipose tissue. Lnc13728 knockdown in hADSCs suppressed the expression of the adipogenesis-related genes at both mRNA and protein level and weakened lipid droplet production. Accordingly, lnc13728 overexpression enhanced hADSC adipogenic differentiation. Beyond that, lnc13728 co-localized with ZBED3 in the cytoplasm and regulated its expression positively. Downregulating ZBED3 had a negative effect on adipogenic differentiation, while the expression of WNT/β-catenin signaling pathway-related proteins was upregulated. Conclusions Lnc13728 promotes hADSC adipogenic differentiation possibly by positively regulating the expression of ZBED3 which plays a role in inhibiting the WNT/β-catenin pathway.
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- 2021
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17. Large-Scale Genomic Epidemiology of Klebsiella pneumoniae Identified Clone Divergence with Hypervirulent Plus Antimicrobial-Resistant Characteristics Causing Within-Ward Strain Transmissions
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Na Pei, Yanming Li, Chunjiao Liu, Zijuan Jian, Tianzhu Liang, Yiming Zhong, Wanying Sun, Jingxuan He, Xinyi Cheng, Hongling Li, Xiaole Lei, Xin Liu, Ziqing Deng, Qingxia Liu, Xia Chen, Qun Yan, Karsten Kristiansen, Junhua Li, and Wenen Liu
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clone divergence ,genomic epidemiology ,Klebsiella pneumoniae ,nosocomial transmission ,Microbiology ,QR1-502 - Abstract
ABSTRACT Global dissemination of K. pneumoniae clones poses health hazards to the public. Genomic epidemiology studies with comprehensive data set further revealed clone divergence, showing a high complexity in evolution. Moreover, clones carrying both acquired virulent and antimicrobial-resistant genes emerged and might replace the carbapenem-resistant clones. Co-occurrence of virulence and resistance is emerging. An unbiased collection of 3,061 clinical K. pneumoniae isolates (January 5, 2013 to July 24, 2018) underwent whole-genome sequencing. Pairwise core-genome single-nucleotide polymorphism (cgSNP) distances identified clone divergence and transmission events. A sum of 2,193 nonduplicated genomes clustered into four phenotypically indistinguishable species complexes. 93% (n = 2,035) were KpI with its largest clonal group (CG) being CG11 (n = 406). Three hundred ninety-three were ST11 and three hundred seventy-four carried blaKPC-2. Noticeably, CG11 is divided into two main subclones based on the capsule synthesis K loci (KL). CG11-KL64 showed a clear hypervirulent plus antimicrobial-resistant (hv+AMR) characteristic. Besides, the phylogenetic structure revealed the clone divergence of CG25, and this is the first report with sufficient CG25 genomes to identify the divergence. The outcomes of the hv+AMR CG25 cluster 1 affected patients were poorer (P < 0.05). Moreover, two episodes of strain transmissions were associated with CG25 cluster 1. Other transmissions were associated with ST20 and ST307. Genomic epidemiology identified clone divergence of CG11 and CG25. The hv+AMR subclones pose greater threats on a global scale. Nosocomial transmissions of the high-risk clones raised our concerns about the evolution and transmission of emerging clones among newborns and critically ill patients. IMPORTANCE The convergence of AMR and acquired virulence posing higher risks to the public is a focusing point. With sufficient genomes and genotypes, we successfully identify the convergence in two subclones, the previously reported CG11-KL64, and the newly reported CG25 cluster 1. The novel finding of the CG25 divergence was not only revealed by the phylogenetic tree but also confirmed by the clinical outcome data and the accessory genome patterns. Moreover, the transmission subclones circulated in two clinically important wards highlights the deficiency of infection control program using conventional methods. Without the assistance of whole-genome sequencing, the transmissions of high-risk clones could not be identified.
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- 2022
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18. The Effect of Adding Transcranial Direct Current Stimulation to Hyperbaric Oxygen Therapy in Patients With Delayed Encephalopathy After Carbon Monoxide Poisoning: A Randomised Controlled Trial
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Huifang Cao, Xiaona Tan, Zibo Liu, Long Zhao, Lin Chi, Manyu Li, Chunhui Liu, and Hongling Li
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transcranial direct current stimulation ,hyperbaric oxygen ,delayed encephalopathy after carbon monoxide poisoning ,cognitive function ,activities of daily living ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Objective: To investigate the effect of transcranial direct current stimulation (tDCS) combined with hyperbaric oxygen therapy (HBOT) in patients with delayed encephalopathy after carbon monoxide poisoning (DEACMP).Design: A parallel-group, open-label randomised controlled study.Setting: Hyperbaric Oxygen Therapy Room of the Second Hospital of Hebei Medical University.Subjects: A total of 40 patients were recruited for the current study. Patients were randomly divided into a treatment group and a control group (20 cases/group).Interventions: Control group: conventional, individualised rehabilitation therapy. Treatment group: conventional, individualised rehabilitation therapy and tDCS.Main Measures: cognitive function of patients, the Barthel Index (BI).Results: After treatment, significantly higher MMSE and BI scores, as well as a greater reduction in P300 latency and an increase in P300 amplitude, were observed in the treatment group compared to the control group (MMSE: 13 ± 7 vs. 9 ± 5; P300 latency: 342 ± 29 vs. 363 ± 17 ms; P300 amplitude: 7.0 ± 3.3 vs. 5.1 ± 2.7 μV; all P < 0.05). In both groups, however, MMSE and BI scores, in addition to P300 amplitude, were significantly improved; in contrast, there was a decrease in P300 latency in both groups after treatment compared to before treatment (all P < 0.05).Conclusion: Combined with HBOT, tDCS can help improve cognitive function and ADL in patients with DEACMP. This combination therapy might be a helpful method to enhance the recovery of patients with DEACMP.
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- 2021
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19. Stability of Wafer-Scale Thin Films of Vertically Aligned Hexagonal BN Nanosheets Exposed to High-Energy Ions and Reactive Atomic Oxygen
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Shiyong Huang, Zhi Kai Ng, Hongling Li, Apoorva Chaturvedi, Jian Wei Mark Lim, Roland Yingjie Tay, Edwin Hang Tong Teo, Shuyan Xu, Kostya (Ken) Ostrikov, and Siu Hon Tsang
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inductively coupled plasmas ,chemical vapor deposition ,boron nitride ,protective layer ,ion bombardment ,atomic oxygen ,Chemistry ,QD1-999 - Abstract
Stability of advanced functional materials subjected to extreme conditions involving ion bombardment, radiation, or reactive chemicals is crucial for diverse applications. Here we demonstrate the excellent stability of wafer-scale thin films of vertically aligned hexagonal BN nanosheets (hBNNS) exposed to high-energy ions and reactive atomic oxygen representative of extreme conditions in space exploration and other applications. The hBNNS are fabricated catalyst-free on wafer-scale silicon, stainless steel, copper and glass panels at a lower temperature of 400 °C by inductively coupled plasma (ICP) assisted chemical vapor deposition (CVD) and subsequently characterized. The resistance of BNNS to high-energy ions was tested by immersing the samples into the plasma plume at the anode of a 150 W Hall Effect Thruster with BNNS films facing Xenon ions, revealing that the etching rate of BNNS is 20 times less than for a single-crystalline silicon wafer. Additionally, using O2/Ar/H2 plasmas to simulate the low Earth orbit (LEO) environment, it is demonstrated that the simulated plasma had very weak influence on the hBNNS surface structure and thickness. These results validate the strong potential of BNNS films for applications as protective, thermally conductive and insulating layers for spacecrafts, electric plasma satellite thrusters and semiconductor optoelectronic devices.
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- 2022
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20. Exosomes secreted by mesenchymal stromal/stem cell-derived adipocytes promote breast cancer cell growth via activation of Hippo signaling pathway
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Shihua Wang, Xiaodong Su, Meiqian Xu, Xian Xiao, Xiaoxia Li, Hongling Li, Armand Keating, and Robert Chunhua Zhao
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Exosomes ,Adipocyte ,Mesenchymal stromal/stem cell ,Breast cancer ,Hippo signaling pathway ,Medicine (General) ,R5-920 ,Biochemistry ,QD415-436 - Abstract
Abstract Objective Although adipocytes are the most abundant stromal cell component in breast cancer tissues, their interaction with breast cancer cells has been less investigated compared to cancer-associated fibroblasts or macrophages. Exosomes are a novel way of cell-cell communication and have been demonstrated to play an important role in various biological processes. However, to our knowledge, only a few studies have reported the effects of adipocyte exosomes on tumor development. Here, utilizing exosomes isolated from in vitro mesenchymal stromal/stem cell (MSC)-differentiated adipocytes, we systematically investigated this issue in a breast cancer model. Material and methods Exosomes were isolated from MSC-differentiated adipocytes and added to breast cancer cells MCF7. Cell proliferation was detected by MTS, and migration was analyzed by wound healing and transwell assay. An in vivo mouse xenograft model was used to evaluate MSC-differentiated adipocyte exosomes’ contribution to tumor growth. Signaling pathway activation was evaluated by western blot and immunofluorescence staining. Results We found MSC-differentiated adipocyte-derived exosomes are actively incorporated by breast cancer cell MCF7 and subsequently promote MCF7 proliferation and migration as well as protect MCF7 from serum derivation or chemotherapeutic drug-induced apoptosis in vitro. In the in vivo mouse xenograft model, depletion of exosomes reduces tumor-promoting effects of adipocytes. Transcriptomic analysis of MSC-differentiated adipocyte exosome-treated MCF7 identified several activated signaling pathways, among which we confirm the Hippo signaling pathway and found a blockade of this pathway leads to a reduced growth-promoting effect of adipocyte exosomes. Conclusion Taken together, our findings provide new insights into the role of adipocyte exosomes in the tumor microenvironment.
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- 2019
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21. Combination of serum lipids and cancer antigens as a novel marker for colon cancer diagnosis
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Tong Li, Yinfen Qian, Hongling Li, and Jiusheng Deng
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Colon cancer ,Serum lipids ,Cancer antigens ,Diagnostic marker ,Nutritional diseases. Deficiency diseases ,RC620-627 - Abstract
Abstract Background Colon cancer is a malignancy of the large intestine with high mortality and economic burden. Recent studies reveal a new relationship between blood lipids and the risk of cancer. The presents study aims to investigate the combination of serum lipids with cancer antigens as a novel diagnostic marker for colon cancer. Methods Two hundred of colon cancer patients or healthy subjects were recruited. Serum lipids and cancer antigens such as total cholesterol (TC), high-density lipoprotein (HDL), carcinoembryonic antigen (CEA) and carbohydrate antigen 19–9 (CA19–9) were measured. Results There were significantly lower level of serum TC or HDL, and significantly higher level of serum CEA or CA19–9 in patients than in healthy subjects. Serum TC or HDL in patients with advanced colon cancer was significantly lower than the ones with early stage disease. The level of serum TC or HDL in patients after surgical removal of colon cancer was significantly higher compared to the ones before surgery, but serum CEA or CA19–9 after surgery was significantly reduced in comparison with the ones before surgery. Combined TC, HDL, CEA and CA19–9 as a diagnostic marker for colon cancer had the highest positive predictive rate in comparison with individual, two or three of the parameters. Conclusions The combination of serum TC, HDL, CEA and CA19–9 can be used as an effective marker for colon cancer, and offers a novel strategy for clinical diagnosis and monitoring the disease.
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- 2018
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22. IMP-38-Producing High-Risk Sequence Type 307 Klebsiella pneumoniae Strains from a Neonatal Unit in China
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Siyi Wang, Juan Zhao, Ning Liu, Fang Yang, Yiming Zhong, Xiumei Gu, Zijuan Jian, Qun Yan, Qingxia Liu, Hongling Li, Yanming Li, Jing Liu, Hui Li, Liang Chen, and Wenen Liu
- Subjects
carbapenem-resistant Klebsiella pneumoniae ,IMP-38 ,ST307 ,whole-genome sequencing ,plasmid ,high-risk clone ,Microbiology ,QR1-502 - Abstract
ABSTRACT An emerging multidrug-resistant Klebsiella pneumoniae high-risk clone of sequence type 307 (ST307) has been increasingly reported worldwide. Here, we described the genomic characteristics of an IMP-38-producing ST307 K. pneumoniae strain and investigated the prevalence of blaIMP-38 among carbapenem-resistant Klebsiella pneumoniae isolates from a tertiary care hospital in central China. A total of 14 IMP-38-producing ST307 K. pneumoniae strains were identified from 2013 to 2016, with 13 strains isolated from patients with neonatal sepsis in the neonatal ward. PacBio and Illumina whole-genome sequencing analysis performed on a representative IMP-38-producing K. pneumoniae strain, WCGKP294, showed that it contained a circular chromosome and two plasmids. Carbapenemase gene blaIMP-38 is colocated with blaCTX-M-3 in transposon Tn6382 on an IncHI5 plasmid (pWCGKP294-2). WCGKP294 harbors another IncFIB plasmid, pWCGKP294-1, carrying three copies of tandem-repeated IS26-blaSHV-2A-deoR-ygbJ-ygbK-fucA-IS26 composite transposon elements. Phylogenetic analysis placed WCGKP294 in the global ST307 cluster, distant from the U.S. (Texas) and South Africa clusters. Nevertheless, WCGKP294 does not contain the chromosomal fluoroquinolone resistance-associated mutations and IncFIIK/IncFIBK plasmid-associated blaCTX-M-15 gene that are frequently found in other global ST307 strains. IMPORTANCE We described the genome and resistome characterization of a carbapenem-resistant Klebsiella pneumoniae ST307 strain carrying blaIMP-38 in China. This report highlights that the high-risk ST307 clone continues to acquire different antimicrobial resistance genes, posing significant challenges to clinical practice, and should be closely monitored.
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- 2020
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23. DHX36, BAX, and ARPC1B May Be Critical for the Diagnosis and Treatment of Tuberculosis
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Yunli Zhang, Yanming Li, Hongling Li, Qingxia Liu, Wei Wang, Zijuan Jian, and Wenen Liu
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Diseases of the respiratory system ,RC705-779 - Abstract
Background. Tuberculosis (TB) is usually caused by Mycobacterium tuberculosis, which has the highest mortality rate among infectious diseases. This study is designed to identify the key genes affecting the diagnosis and treatment of TB. Methods. GSE54992, which included 39 peripheral blood mononuclear cell (PBMC) samples, was extracted from the Gene Expression Omnibus database. After the samples were classified into type and time groups by limma package, the differentially expressed genes (DEGs) were analyzed using the Analysis of Variance. Using pheatmap package, hierarchical cluster analysis was performed for the DEGs. Then, the key modules correlated with TB were selected using the WGCNA package. Finally, functional and pathway enrichment analyses were carried out using clusterProfiler package. Results. The DEGs in subclusters 3, 6, 7, and 8 were chosen for further analyses. Based on WGCNA analysis, blue and green modules in type group and pink module in time group were selected as key modules. From the key modules, 9 (including BAX and ARPC1B) hub genes in type group and 6 (including DHX36) hub genes in time group were screened. Through pathway enrichment analysis, the TNF signaling pathway was enriched for the green module. Conclusion. DHX36, BAX, and ARPC1B might be key genes acting in the mechanisms of TB. Besides, the TNF signaling pathway might also be critical for the diagnosis and therapy of the disease.
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- 2020
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24. Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates from 17 Chinese cities from 2011 to 2016
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Chunjiang Zhao, Zongbo Li, Feifei Zhang, Xiaobing Zhang, Ping Ji, Ji Zeng, Bijie Hu, Zhidong Hu, Kang Liao, Hongli Sun, Rong Zhang, Bin Cao, Chao Zhuo, Wei Jia, Yaning Mei, Yunzhuo Chu, Xuesong Xu, Qing Yang, Yan Jin, Quan Fu, Xiuli Xu, Hongling Li, Lijun Wang, Yuxing Ni, Hongjie Liang, and Hui Wang
- Subjects
Streptococcus pneumoniae ,Serotyping ,Antimicrobial resistance ,Multilocus sequence typing ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Streptococcus pneumoniae, the leading pathogen of bacterial infections in infants and the elderly, is responsible for pneumococcal diseases with severe morbidity and mortality. Emergence of drug-resistant strains presented new challenges for treatment and prevention. Vaccination has proven to be an effective means of preventing pneumococcal infection worldwide. Detailed epidemiological information of antibiotic susceptibilities and serotype distribution will be of great help to the management of pneumococcal infections. Methods A total of 881 S. pneumoniae isolates were collected from patients at 23 teaching hospitals in 17 different cities from 2011 to 2016. The main specimen types included sputum, blood, broncho-alveolar lavage fluid, pharyngeal swabs, and cerebrospinal fluid. Minimum inhibitory concentrations (MICs) were determined using the agar dilution method. Capsular serotypes were identified using latex agglutination and quellung reaction test. Molecular epidemiology was investigated using multilocus sequence typing. Results S. pneumoniae isolates were highly resistant to macrolides, tetracycline, and trimethoprim/sulfamethoxazole. The rate of resistance to penicillin was 51.6% (oral breakpoint). However, levofloxacin and moxifloxacin maintained excellent antimicrobial activity and all of the isolated strains were susceptible to vancomycin. Twenty-two serotypes were identified among the 881 isolates. Prevalent serotypes were 19F (25.7%), 19A (14.0%), 15 (6.8%), 6B (3.6%), 6A (3.0%), and 17 (2.8%). The overall vaccine coverage rates for 7- and 13-valent pneumococcal conjugate vaccines were 37.5% and 58.3%, respectively. Vaccine coverage rates in young children and economically underdeveloped regions were higher than those in older adults and developed regions. Vaccine-covered serotypes demonstrated higher resistance compared with uncovered serotypes. Molecular epidemiological typing demonstrated that S. pneumoniae showed significant clonal dissemination and that ST271 (120, 28.3%), ST320 (73, 17.2%) and ST81 (27, 6.6%) were the major STs. Conclusions High resistance to clinical routine antibiotics was observed for all 881 S. pneumoniae strains. Drug resistance varied among different serotypes and age groups. Prevalent serotypes among the isolates were 19F, 19A, 15, 6B, 6A, and 17. Community-acquired strains should also be included in future studies to gain a better understanding of the prevalence and resistance of S. pneumoniae in China.
- Published
- 2017
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25. Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway
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Xiaoxia Li, Shihua Wang, Rongjia Zhu, Hongling Li, Qin Han, and Robert Chunhua Zhao
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Exosomes ,MSCs ,Tumor-supportive ,Inflammation ,NF-κB ,TLR2 ,Diseases of the blood and blood-forming organs ,RC633-647.5 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background In tumor microenvironment, a continuous cross-talk between cancer cells and other cellular components is required to sustain tumor progression. Accumulating evidence suggests that exosomes, a novel way of cell communication, play an important role in such cross-talk. Exosomes could facilitate the direct intercellular transfer of proteins, lipids, and miRNA/mRNA/DNAs between cells. Since mesenchymal stem cells (MSCs) can be attracted to tumor sites and become an important component of the tumor microenvironment, there is an urgent need to reveal the effect of tumor exosomes on MSCs and to further explore the underlying molecular mechanisms. Methods Exosomes were harvested from lung cancer cell line A549 and added to MSCs. Secretion of inflammation-associated cytokines in exosome-treated MSCs were analyzed by RT-PCR and ELISA. The growth-promoting effect of exosome-treated MSCs on lung tumor cells was evaluated by in vivo mouse xenograft model. Signaling pathway involved in exosomes-treated MSCs was detected by PCR array of human toll-like receptor signaling pathway, RT-PCR, and Western blot. Results Data showed that lung tumor cell A549-derived exosomes could induce a pro-inflammatory phenotype in MSCs named P-MSCs, which have significantly elevated secretion of IL-6, IL-8, and MCP-1. P-MSCs possess a greatly enhanced ability in promoting lung tumor growth in mouse xenograft model. Analysis of the signaling pathways in P-MSCs revealed a fast triggering of NF-κB. Genetic ablation of Toll-like receptor 2 (TLR2) by siRNA and TLR2-neutralizing antibody could block NF-κB activation by exosomes. We further found that Hsp70 present on the surface of lung tumor exosomes contributed to the induction of P-MSCs by A549 exosomes. Conclusions Our studies suggest a novel mechanism by which lung tumor cell-derived exosomes induce pro-inflammatory activity of MSCs which in turn get tumor supportive characteristics.
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- 2016
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26. Long Noncoding RNA ADINR Regulates Adipogenesis by Transcriptionally Activating C/EBPα
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Tengfei Xiao, Lihui Liu, Hongling Li, Yu Sun, Huaxia Luo, Tangping Li, Shihua Wang, Stephen Dalton, Robert Chunhua Zhao, and Runsheng Chen
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Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
C/EBPα is a critical transcriptional regulator of adipogenesis. How C/EBPα transcription is itself regulated is poorly understood, however, and remains a key question that needs to be addressed for a complete understanding of adipogenic development. Here, we identify a lncRNA, ADINR (adipogenic differentiation induced noncoding RNA), transcribed from a position ∼450 bp upstream of the C/EBPα gene, that orchestrates C/EBPα transcription in vivo. Depletion of ADINR leads to a severe adipogenic defect that is rescued by overexpression of C/EBPα. Moreover, we reveal that ADINR RNA specifically binds to PA1 and recruits MLL3/4 histone methyl-transferase complexes so as to increase H3K4me3 and decrease H3K27me3 histone modification in the C/EBPα locus during adipogenesis. These results show that ADINR plays important roles in regulating the differentiation of human mesenchymal stem cells into adipocytes by modulating C/EBPα in cis.
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- 2015
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27. Synthesis of Ergosterol Peroxide Conjugates as Mitochondria Targeting Probes for Enhanced Anticancer Activity
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Ming Bu, Hongling Li, Haijun Wang, Jing Wang, Yu Lin, and Yukun Ma
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ergosterol peroxide ,target probe ,mitochondria ,fluorescence imaging ,antitumor activity ,Organic chemistry ,QD241-441 - Abstract
Inspired by the significant bioactivity of ergosterol peroxide, we designed and synthesized four fluorescent coumarin and ergosterol peroxide conjugates 8a−d through the combination of ergosterol peroxide with 7-N,N-diethylamino coumarins fluorophore. The cytotoxicity of synthesized conjugates against three human cancer cells (HepG2, SK-Hep1, and MCF-7) was evaluated. The results of fluorescent imaging showed that the synthesized conjugates 8a−d localized and enriched mainly in mitochondria, leading to significantly enhanced cytotoxicity over ergosterol peroxide. Furthermore, the results of biological functions of 8d showed that it could suppress cell colony formation, invasion, and migration; induce G2/M phase arrest of HepG2 cells, and increase the intracellular ROS level.
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- 2019
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28. Comment on 'Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing'
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Hongling Li, Cong Cao, Ai Huang, and Yi Man
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
A recent paper in this journal, presented a novel method by topical application of growth factors in stimulating diabetic cutaneous wound healing that caught our attention. We believe that the experimental method in the article is efficient and creative, but it also has some controversies and shortcomings to be discussed. We noted that the authors used “Tegaderm” as a semiocclusive dressing film and stated that it exerted a “splinting effect” on the wound margins and controlled contraction. Indeed, the “Tegaderm” itself can serve as a dressing film to isolate the wound bed with outside environments while the “splinting effect” is mainly achieved by adding silicone splints around the wound. Considering the unique properties of silicone splints and “Tegaderm,” our experimental group propose an alternative method named “combined-suturing” technique that is not only suturing the silicone splints but also securing the “Tegaderm” around the wound. The specific reasons and operative procedures are explained in detail in this letter.
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- 2015
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29. The Epstein-Barr virus-miRNA-BART6-5p regulates TGF-β/SMAD4 pathway to induce glycolysis and enhance proliferation and metastasis of gastric cancer cells.
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XUHUI ZHAO, XIAOMIN HUANG, CHUNYAN DANG, XIA WANG, YUJIAO QI, and HONGLING LI
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STOMACH cancer ,CANCER cells ,GLYCOLYSIS ,METASTASIS ,CANCER cell migration - Abstract
Background: EBV-miR-BARTs exhibit significant relevance in epithelial tumors, particularly in EBVassociated gastric and nasopharyngeal cancers. However, their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored. Material and Methods: Initially, EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines. Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45, and downregulation in EBVpositive cells (SUN-719). The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation, migration, and glycolytic processes, with the TGF-ß/SMAD4 signaling pathway value clarified using a TGF-ß inhibitor. Results: EBV-miRNA-BART6-5p exhibits pronounced upregulation in EBV-associated gastric cancer tissues and EBV-positive cells, while its target gene SMAD4 demonstrates downregulated expression. Upregulation of it can promote the proliferation and migration of gastric cancer cells. Additionally, We found EBV-miRNA-BART6-5p promotes glycolysis of gastric cancer cells. Inhibition of the TGF-ß/SMAD4 signaling pathway resulted in suppressed proliferation and migration of gastric cancer cells, concomitant with a diminished glycolytic capacity. Conclusion: In this study, we found that EBV-miRNA-BART6-5p can target SMAD4, effectively increasing glycolysis in gastric cancer cells by regulating the TGF-ß/SMAD4 signaling pathway, thereby enhancing the proliferation and metastasis of gastric cancer cells. Our findings may offer new insights into the metabolic aspects of gastric cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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30. Uterine tumors mimicking ovarian sex cord tumors with rhabdoid differentiation: a clinicopathologic study of 4 cases: A case series analysis.
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Hongling Li, Le Xie, Jinhui Zhang, Yuanyuan Xu, Xingyan Wu, Zengwei Chen, and Rongjun Mao
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- 2024
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31. YNU_oxz at SemEval-2020 Task 12: Bidirectional GRU with Capsule for Identifying Multilingual Offensive Language.
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Xiaozhi Ou and Hongling Li
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- 2020
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32. YNU-oxz at SemEval-2020 Task 4: Commonsense Validation Using BERT with Bidirectional GRU.
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Xiaozhi Ou and Hongling Li
- Published
- 2020
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33. Zyy1510 Team at SemEval-2020 Task 9: Sentiment Analysis for Code-Mixed Social Media Text with Sub-word Level Representations.
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Yueying Zhu, Xiaobing Zhou, Hongling Li, and Kunjie Dong
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- 2020
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34. YNU-oxz at SemEval-2020 Task 5: Detecting Counterfactuals Based on Ordered Neurons LSTM and Hierarchical Attention Network.
- Author
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Xiaozhi Ou, Shengyan Liu, and Hongling Li
- Published
- 2020
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35. YNU@Dravidian-CodeMix-FIRE2020: XLM-RoBERTa for Multi-language Sentiment Analysis.
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Xiaozhi Ou and Hongling Li
- Published
- 2020
36. YNU_OXZ at HASOC 2020: Multilingual Hate Speech and Offensive Content Identification based on XLM-RoBERTa.
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Xiaozhi Ou and Hongling Li
- Published
- 2020
37. AN ALTERNATIVE OPTIMISATION MODEL FOR RESERVE CAPACITY OF POWER SYSTEM CONSIDERING COORDINATIVE AGGREGATION OF LARGE-SCALE RENEWABLE ENERGY, 1-6.
- Author
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Bin, Wei, primary, Chao, Gao, additional, Huazhen, Cao, additional, Changwei, Wang, additional, Jiekang, Wu, additional, and Hongling, Li, additional
- Published
- 2024
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38. Combined with finite element analysis of car seat safety performance improvement
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Hongling, Li, Yude, Dong, Changliang, Chen, and Chao, Chen
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- 2021
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39. YNU_OXZ @ HaSpeeDe 2 and AMI : XLM-RoBERTa with Ordered Neurons LSTM for Classification Task at EVALITA 2020.
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Xiaozhi Ou and Hongling Li
- Published
- 2020
40. 基于贝叶斯网的短文本特征扩展方法 (Short Text Feature Extension Method Based on Bayesian Networks).
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Huiqing Liu, Yanbu Guo, Hongling Li, and Weihua Li 0006
- Published
- 2019
41. Effect of transcranial direct current stimulation combined with respiratory training on dysphagia in post-stroke patients
- Author
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Hao, Li, Long, Zhao, Xiaokai, Yuan, Qingjuan, Zhang, Yatao, Pang, and Hongling, Li
- Subjects
Biomaterials ,Biomedical Engineering ,Biophysics ,Health Informatics ,Bioengineering ,Information Systems - Abstract
BACKGROUND: Transcranial direct current stimulation (tDCS) has a considerable advantage in the rehabilitation treatment of dysphagia. OBJECTIVE: The purpose of this study was to explore the effect of tDCS combined with respiratory training on dysphagia in post-stroke patients. METHODS: From December 2017 to January 2019, 64 post-stroke patients who were hospitalized in the Department of Neurology of the Second Hospital of Hebei Medical University were enrolled in this study. They were randomly divided into control and treatment groups (n= 32). Patients in the two groups received routine swallowing rehabilitation training and respiratory training. On this basis, the patients in the treatment group received tDCS. The anode was placed in the movement area of the pharyngeal cortex on the unaffected side of the patients’ bodies, and the cathode was placed in the upper orbital orbit on the opposite side. The current intensity was 1.5 mA, 20 min/time, 1 time/d, and 6 d/w. Before and after the treatment, the water swallow text, functional oral intake scale (FOIS), forced vital capacity (FVC) and peak expiratory flow (PEF) were assessed, and the correlation among them was evaluated. RESULTS: There were no differences in all indexes before and after treatment. After treatment, water swallow text, FOIS, FVC and PEF were all better than before treatment, and the clinical efficacy in the treatment group was significantly better than that in the control group. FVC and PEF were positively correlated with water swallow text and FOIS. CONCLUSION: tDCS combined with respiratory training may have a significant therapeutic effect on dysphagia in post-stroke patients.
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- 2023
42. The effect of radiotherapy time and dose on acute hematologic toxicity during concurrent postoperative chemoradiotherapy for early high-risk cervical cancer
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Xiaojing Yang, Zhen Li, Lihua Zhang, Hongling Li, Xinmiao Yang, Yi Sun, Lijun Liu, and Jie Fu
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Oncology - Published
- 2023
43. Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma
- Author
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Dezhuan, Da, Zhiang, Pan, Lu, Zeng, Yamei, Dang, Chunyan, Dang, Yunxia, Huang, Dujuan, Shi, and Hongling, Li
- Subjects
Stomach Neoplasms ,Glutamate-Cysteine Ligase ,Humans ,Surgery ,Adenocarcinoma ,Glutathione - Abstract
To determine the expression and function of glutamate-cysteine ligase catalytic (GCLC) and glutamate-cysteine ligase catalytic modifier (GCLM) in gastric adenocarcinoma.Bioinformatics was used to analyze the expression of GCLC and GCLM. We download and analyzed the expression of gastric adenocarcinoma patients from TCGA database. Moreover, the method of immunochemistry was used to verify the expression of GCLC and GCLM in gastric adenocarcinoma.At first, the expression of GCLC and GCLM in gastric adenocarcinoma tissues were both significantly higher compared with normal tissues analyzed via TCGA database. Then, gastric adenocarcinoma tissues were collected and performed with immunochemistry. The gastric adenocarcinoma with positive staining for GCLC and GCLM was 77% and 80%, respectively, which was significantly higher compared with adjacent normal tissues (9% and 11%, respectively).The disordered expression of GCLC and GCLM in gastric adenocarcinoma suggested that these factors may induce tumorigenesis and may be a novel target for diagnosis and treatment of gastric adenocarcinoma.
- Published
- 2023
44. PG-CODE: Latent dirichlet allocation embedded policy knowledge graph for government department coordination
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Yilin Kang, Renwei Ou, Yi Zhang, Hongling Li, and Shasha Tian
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Multidisciplinary - Published
- 2022
45. Successful treatment with bortezomib in combination with dexamethasone in a middleaged male with idiopathic multicentric Castleman’s disease: A case report.
- Author
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Hongling Li, Yang He, Yongying Wang, and Mengwei Xu
- Abstract
Multicentric Castleman disease (MCD) is a heterogeneous, life-threatening disease. A subgroup of HIV-negative and HHV-8-negative MCD is defined as idiopathic MCD (iMCD) with a poor prognosis. Here we report an unusual case of a 47-year-old male patient with iMCD who experienced multiple treatment regimens such as chemotherapy, immunomodulatory therapy, and targeted therapy, all of which were considered ineffective. Subsequently, he was started on bortezomib in combination with dexamethasone for six cycles and he was in complete remission. The patient has survived nearly 13 years to date – the longest survival of any iMCD patient treated with bortezomib in combination with dexamethasone. Bortezomib combined with dexamethasone may be an effective salvage strategy for severe and refractory iMCD. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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46. Value of Combined Detection of PCT, CRP, and FIB in Differentiating Viral Infection from Bacterial Infection in Severe Pneumonia.
- Author
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JiaQian Ma, LingLing Li, XiaoLiang Qie, Qian Zhao, LiTao Zhang, Ning Xu, XuRui Li, Hui Guo, HongLing Li, Jian Lv, and JianGuo Li
- Subjects
BACTERIAL diseases ,VIRUS diseases ,PEARSON correlation (Statistics) ,RECEIVER operating characteristic curves ,NEONATAL sepsis ,C-reactive protein - Abstract
Background: The aim was to explore the value of combined detection of PCT, CRP, and FIB in differentiating severe pneumonia from viral infection and bacterial infection. Methods: A total of 100 patients with severe pneumonia admitted to Hebei General Hospital from August 2020 to November 2021 were selected as the research objects, including 50 patients with viral pneumonia (as the viral group, n = 50) and 50 patients with bacterial pneumonia (as the bacterial group, n = 50). At the same time, the clinical data of 50 healthy people in the hospital were selected as the healthy group (n = 50). All the subjects in the three groups were tested for PCT, CRP, and FIB. The difference of each index level among the three groups was compared. The diagnostic efficacy of each index for pneumonia was analyzed by drawing receiver operating characteristic curves, and the independent predictors of pneumonia were determined by logistic regression model. Results: There were no statistically significant differences in gender, age, course of disease, body mass index (BMI), and other general data among the three groups (p > 0.05). Compared with the healthy group, the levels of serum PCT, CRP, and FIB in the viral group and the bacterial group were significantly increased, and the levels of serum PCT, CRP, and FIB in the bacterial group were significantly higher than those in the viral group, and the differences were statistically significant (p < 0.05). The positive rates of FIB, CRP, and PCT in bacterial group and viral group were increased in turn, and the differences were statistically significant (p < 0.05), and the positive rates of combined detection in the two groups were significantly higher than the positive rates of single index detection (p < 0.05). Taking etiological examination as the gold standard, the sensitivity (92.59%) and specificity (90.17%) of the three combined detection methods were significantly higher than those of PCT, CRP, and FIB alone. Kappa test showed that the results of the combined detection and etiological examination were in good agreement (Kappa value = 0.847, p < 0.05). ROC curve analysis showed that the AUC of combined prediction of the three was 0.964, which was higher than that of single detection of 0.859, 0.832, and 0.871. Logistic regression analysis showed that serum PCT, CRP, and FIB were independent predictors of bacterial pneumonia, and the differences were statistically significant (p < 0.05). Pearson's correlation analysis showed that FIB level in the bacterial group was positively correlated with PCT and CRP. PCT was positively correlated with CRP. Conclusions: Compared with viral pneumonia, the levels of serum PCT, CRP, and FIB in patients with bacterial pneumonia are higher. Biochemical indexes can be used as independent predictors for the diagnosis of bacterial pneumonia, and have high diagnostic value. The combined detection of the three has the highest diagnostic efficiency, which is conducive to the clinical differential diagnosis of the early types of pneumonia infection. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
47. Honeynet optimization strategy based on attack graph
- Author
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ZhangSheng Yang, HongLing Li, Zhe Yang, and LiuZhi Ye
- Published
- 2023
48. Out-degree betweenness centrality based on betweenness centrality
- Author
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Zhe Yang, HongLing LI, ZhangSheng Yang, and LiuZhi Ye
- Published
- 2023
49. Hiding information in BMP based on ECC and LSB algorithm
- Author
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Liuzhi Ye, Hongling Li, Zhangsheng Yang, and Zhe Yang
- Published
- 2023
50. Increased serum anti-CYP2E1 IgG autoantibody levels may be involved in the pathogenesis of occupational trichloroethylene hypersensitivity syndrome: a case–control study
- Author
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Tamie Nakajima, Hailan Wang, Yuan Yuan, Yuki Ito, Hisao Naito, Yoshiyuki Kawamoto, Kozue Takeda, Kiyoshi Sakai, Na Zhao, Hongling Li, Xinxiang Qiu, Lihua Xia, Jiabin Chen, Qifeng Wu, Laiyu Li, Hanlin Huang, Yukie Yanagiba, Hiroshi Yatsuya, and Michihiro Kamijima
- Subjects
Male ,Polymorphism, Genetic ,Health, Toxicology and Mutagenesis ,Cytochrome P-450 CYP2E1 ,General Medicine ,Toxicology ,Trichloroethylene ,Hepatitis, Autoimmune ,HLA-B Antigens ,Case-Control Studies ,Immunoglobulin G ,Occupational Exposure ,Drug Hypersensitivity Syndrome ,Humans ,Female ,Chemical and Drug Induced Liver Injury ,Autoantibodies - Abstract
Occupational exposure to trichloroethylene (TCE) causes a systemic skin disorder with hepatitis known as TCE hypersensitivity syndrome (TCE-HS). Human Leukocyte Antigen (HLA)-B*13:01 is its susceptibility factor; however, the immunological pathogenesis of TCE-HS remains unknown. We herein examined the hypothesis that autoantibodies to CYP2E1 are primarily involved in TCE-HS. A case–control study of 80 TCE-HS patients, 186 TCE-tolerant controls (TCE-TC), and 71 TCE-nonexposed controls (TCE-nonEC) was conducted to measure their serum anti-CYP2E1 antibody (IgG) levels. The effects of TCE exposure indices, such as 8-h time-weighted-average (TWA) airborne concentrations, urinary metabolite concentrations, and TCE usage duration; sex; smoking and drinking habits; and alanine aminotransferase (ALT) levels on the antibody levels were also analyzed in the two control groups. There were significant differences in anti-CYP2E1 antibody levels among the three groups: TCE-TC > TCE-HS patients > TCE-nonEC. Antibody levels were not different between HLA-B*13:01 carriers and noncarriers in TCE-HS patients and TCE-TC. The serum CYP2E1 measurement suggested increased immunocomplex levels only in patients with TCE-HS. Multiple regression analysis for the two control groups showed that the antibody levels were significantly higher by the TCE exposure. Women had higher antibody levels than men; however, smoking, drinking, and ALT levels did not affect the anti-CYP2E1 antibody levels. Anti-CYP2E1 antibodies were elevated at concentrations lower than the TWA concentration of 2.5 ppm for TCE exposure. Since HLA-B*13:01 polymorphism was not involved in the autoantibody levels, the possible mechanism underlying the pathogenesis of TCE-HS is that TCE exposure induces anti-CYP2E1 autoantibody production, and HLA-B*13:01 is involved in the development of TCE-HS.
- Published
- 2022
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