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Objective This study aims to investigate the effect of Bacillus subtilis WB800N on diabetic wounds. Methods Haematoxylin & eosin (H&E) staining was used to observe the healing of skin wounds. Collagen deposition was assessed by Masson staining. Western blotting and qRT-PCR were used to detect vascular endothelial-related factors (VWF), CD31, TLR2, NLRP3, ASC and Caspase-1 expression. 16S rDNA sequencing detected microbiota distribution. The concentrations of IL-1β and IL-37 were measured by ELISA. Apoptosis was measured by the TUNEL assay. Results Compared with the control group, wound healing was delayed in diabetic mice. The wound area in the Bacillus subtilis group decreased more significantly than the diabetic wound group. H&E staining showed that Bacillus subtilis WB800N promoted wound healing and increased re-epithelialization. Masson staining showed that Bacillus subtilis WB800N increased collagen deposition in mice with diabetic wounds. Bacillus subtilis WB800N upregulated VWF and CD31 protein expression in diabetic wounds mice. The 16S rDNA results showed that Bacillus subtilis WB800N reduced the diversity of the gut microbiota of diabetic wounds mice and regulated the microbial composition. At the genus level, Bacillus subtilis WB800N reduced the relative abundance of Muribaculaceae and CG − 005 in diabetic wounds mice, whilst increasing the relative abundance of Lactobacillus. Bacillus subtilis WB800N increased the expression of TLR2, NLRP3, ASC and Caspase-1. Bacillus subtilis WB800N increased the concentrations of IL-1β and IL-37 in serum. Bacillus subtilis WB800N upregulated cell apoptosis. The TLR2 antagonist Sparstolonin B (SsnB) reduced the expression of TLR2, NLRP3, ASC, Caspase-1, IL-1β and IL-37 and the apoptosis in diabetic wounds mice, whilst the combined intervention of Bacillus subtilis and SsnB reversed the effect of SsnB treatment alone. Conclusion Bacillus subtilis WB800N alleviated diabetic wound healing by regulating gut microbiota homeostasis and TLR2. Significance and impact of research Our findings might provide potential therapeutic targets for diabetic wounds.

BACKGROUND: With the development of breast augmentation and the increased people's pursuit of beauty, more and more women choose breast prosthesis breast augmentation. This study aimed to evaluate the incidence of capsular contracture after breast augmentation with textured and smooth breast prostheses by meta-analysis. METHODS: Studies relating to capsular contracture after breast augmentation with textured breast prosthesis and smooth breast prosthesis were retrieved from the Chinese Journal Full-text Database (CNKI), VIP, the Wanfang Science and Technology Journal Full-text Database, the Chinese Biomedical Literature Search Database (CBM), PubMed, Embase, and the Cochrane Library. The search period was from database establishment to October 2021. EndNote X9 software (Clarivate, Philadelphia, PA, USA) was used to screen and exclude duplicate studies, screen the studies according to the inclusion and exclusion criteria, and extract data for quality evaluation. The data were recorded using the Stata 15.1 software (StataCorp LLC, College Station, TX, USA), and a meta-analysis was performed on the incidence of capsular contracture after breast augmentation with textured and smooth breast prostheses. Finally, the reliability of the results was assessed by sensitivity analysis. A funnel plot was used to evaluate the publication bias of the literature. RESULTS: A total of 13 articles were included, and the total sample size of the study was 5,440. The results of the meta-analysis showed that the incidence of capsular contracture after breast augmentation with textured breast prostheses was significantly lower than that with smooth breast prostheses (OR =0.26; 95% CI: 0.08, 0.81; P

Author(s): Liao, Junlin; Chen, Yong; Chen, Jia; He, Bin; Qian, Li; Xu, Jiaqin; Wang, Aijun; Li, Qingfeng; Xie, Hongju; Zhou, Jianda | Abstract: ObjectiveTo reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP).MethodsMaterialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness.ResultsThe 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P g 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans.ConclusionAuricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties.Levels of evidenceNA. Laryngoscope, 129:2467-2474, 2019.

Although the original purpose of the systemic lupus erythematosus (SLE) classification criteria was to distinguish SLE from other mimic diseases, and to facilitate sample selection in scientific research, they have become widely used as diagnostic criteria in clinical situations. It is not known yet if regarding classification criteria as diagnostic criteria, what problems might be encountered? This is the first study comparing the three sets of classification criteria for SLE, the 1997 American College of Rheumatology (ACR’97), 2012 Systemic Lupus International Collaborating Clinics (SLICC’12) and 2019 European League Against Rheumatism/American College of Rheumatology (EULAR/ACR’19), for their ability to distinguish patients with SLE from patients with pure mucocutaneous manifestations (isolated cutaneous lupus erythematosus without internal disease, i-CLE) in the lupus disease spectrum. 1,865 patients with SLE and 232 patients with i-CLE were recruited from a multicenter study. We found that, due to low specificity, none of the three criteria are adept at distinguishing patients with SLE from patients with i-CLE. SLICC’12 performed best among the original three criteria, but if a positive ANA was removed as an entry criterion, EULAR/ACR’19 would performed better. A review of previous studies that compared the three sets of criteria was presented in this work.

Supplemental material, sj-pdf-1-lup-10.1177_0961203320959716 for A comparison and review of three sets of classification criteria for systemic lupus erythematosus for distinguishing systemic lupus erythematosus from pure mucocutaneous manifestations in the lupus disease spectrum by Hui Jin, Tao Huang, Ruifang Wu, Ming Zhao, Haijing Wu, Hai Long, Heng Yin, Jieyue Liao, Shuangyan Luo, Yu Liu, Qing Zhang, Peng Zhang, Yixin Tan, Shuaihantian Luo, Xin Huang, Yaxiong Deng, Wei Liao, Liu Duan, Jianbo Chen, Yin Zhou, Jinghua Yin, Hong Qiu, Jin Yuan, Zijun Wang, Mengying Li, Xiaoqi Wu, Lina Chen, Liangmin Cai, Cancan Huang, Qianwen Li, Bingsi Tang, Bihui Yu, Xin Li, Xiaofei Gao, Yixi Hu, Xiaolei Ren, Haofan Xue, Zhangming Wei, Jinwei Chen, Fen Li, Guanghui Ling, Hui Luo, Hongjun Zhao, Sen Yang, Yong Cui, Youkun Lin, Xu Yao, Lingyun Sun, Qing Guo, Hong Fang, Kang Zeng, Danqi Deng, Jianzhong Zhang, Yuzhen Li, Xiongming Pu, Xiangping Liao, Xiqiang Dang, Danlin Huang, Yumei Liang, Qing Sun, Hongju Xie, Li Zeng, Cibo Huang, Qingchun Diao, Juan Tao, Jianbin Yu, Zhenlu Li, Hanshi Xu, Hui Li, Wei Lai, Xiguang Liu, Jingjing Wu, Tienan Li, Tiechi Lei, Qiuning Sun, Yanjia Li, Guoqiang Zhang, Xin Huang and Qianjin Lu in Lupus

Supplemental material, sj-pdf-2-lup-10.1177_0961203320959716 for A comparison and review of three sets of classification criteria for systemic lupus erythematosus for distinguishing systemic lupus erythematosus from pure mucocutaneous manifestations in the lupus disease spectrum by Hui Jin, Tao Huang, Ruifang Wu, Ming Zhao, Haijing Wu, Hai Long, Heng Yin, Jieyue Liao, Shuangyan Luo, Yu Liu, Qing Zhang, Peng Zhang, Yixin Tan, Shuaihantian Luo, Xin Huang, Yaxiong Deng, Wei Liao, Liu Duan, Jianbo Chen, Yin Zhou, Jinghua Yin, Hong Qiu, Jin Yuan, Zijun Wang, Mengying Li, Xiaoqi Wu, Lina Chen, Liangmin Cai, Cancan Huang, Qianwen Li, Bingsi Tang, Bihui Yu, Xin Li, Xiaofei Gao, Yixi Hu, Xiaolei Ren, Haofan Xue, Zhangming Wei, Jinwei Chen, Fen Li, Guanghui Ling, Hui Luo, Hongjun Zhao, Sen Yang, Yong Cui, Youkun Lin, Xu Yao, Lingyun Sun, Qing Guo, Hong Fang, Kang Zeng, Danqi Deng, Jianzhong Zhang, Yuzhen Li, Xiongming Pu, Xiangping Liao, Xiqiang Dang, Danlin Huang, Yumei Liang, Qing Sun, Hongju Xie, Li Zeng, Cibo Huang, Qingchun Diao, Juan Tao, Jianbin Yu, Zhenlu Li, Hanshi Xu, Hui Li, Wei Lai, Xiguang Liu, Jingjing Wu, Tienan Li, Tiechi Lei, Qiuning Sun, Yanjia Li, Guoqiang Zhang, Xin Huang and Qianjin Lu in Lupus

OBJECTIVE To assess the viability and biomechanics of bare diced cartilage grafts. METHODS Cartilage samples were collected from 1 ear in 15 rabbits as well as costal cartilage. Each rabbit was inserted bare diced- and single-strip costal-cartilage grafts, respectively, into paraspinal subcutaneous pockets: after euthanasia at 2 months, specimens were weighed, with diced cartilage grafts examined histomorphologically by hematoxylin-eosin staining, masson trichrome staining, and immunohistochemistry. Finally, biomechanical properties of grafts were assessed. RESULTS Bare diced cartilage grafts were connected into an integrated mass after 2 months, and inward growth of fibrous tissues and angiogenesis were observed. Mean wet weights of diced cartilage grafts were 1.603 ± 0.278 and 1.662 ± 0.204 g pre- and postoperation, respectively; those of costal cartilage grafts were 0.053 ± 0.008 and 0.058 ± 0.008 g, respectively. In compression assays, mean modulus values of elasticity at yield in diced- and costal-cartilage grafts were 7.65 ± 0.59 and 22.30 ± 1.15 MPa, respectively (P

To evaluate the clinical effect of combination of autologous tissue reconstruction of tarsal plate with temporal flap on repair of full-thickness lower eyelid defect. Methods: Eleven patients (11 eyes) underwent hard palate mucosa or ear cartilage combined the emporal flap with the orbicularis oculi muscle to repair full-thickness defect of palpebra inferior. Of the 11 patients, 6 had more than 75% eyelid defect area, and 5 had more than 50% eyelid defect area. Results: All 11 eyes closed completely, with no entropion or ectropion, and returned to normal basically. Postoperative follow-up was performed for 6 months to 5 years, 3 years and 4 months on average. The function and form of eyelid remained stable. Infection, leakage or contracture was not found on reconstruction tarsus. Conclusion: Reconstruction of eyelid with autogenous hard palate mucosa or ear cartilage combined the emporal flap with the orbicularis oculi muscle is a simple, convenient and effective method.目的：总结和评价下眼睑全层缺损创伤后采用自体硬腭黏膜或耳软骨替代眼睑后层，联合眼轮匝肌蒂颞区皮瓣修复眼睑再造的临床效果。方法：对11例(11眼)下眼睑创伤后患者行自体硬腭黏膜或自体耳软骨联合眼轮匝肌蒂颞区皮瓣修复下眼睑全层缺损。11例均为重度眼睑缺损，其中眼睑75%以上全层缺损6例，50%缺损5例。 结果：11眼睑术后功能及外观基本恢复正常，闭合完全，无内外翻。术后随访6个月~5年，平均3年4个月，眼睑功能及形态保持稳定，再造睑板无感染、外露及挛缩。结论：自体硬腭黏膜或耳软骨联合眼轮匝肌蒂颞区皮瓣下眼睑重建术是一种简便易行、取材方便、临床效果满意的眼睑再造术。.

To explore effects of trehalose as a cryopreserve agent on survival rate of fatty tissue after cryopreservation. Methods: The liposuction was used on the abdomen of adult female. After centrifugation and purification, adipose was randomized into the following three groups, the trehalose group, the fetal bovine serum (FBS)+10%DMSO group and the physiological saline group. The specimens were cryopreserved at -196 ℃ for 3 months and then the HE staining, glucose transfer method and CK method were used to detect the cell survival rate in each group. Results: The activity of adipose in the trehalose group and FBS+10%DMSO group adipose was higher than that in the physiological saline group (P0.05); while there was no significant difference between the trehalose group and FBS+10%DMSO group (P0.05). Conclusion: As cryoprotectant, trehalose could keep fat cell viability, and adipose tissue can be used for clinical transplantation after 3 months' freezing.目的：探讨海藻糖作为冻存保护剂对脂肪组织冻存后存活率的影响。方法：抽吸成年女性腹部或大腿脂肪颗粒，经离心纯化后，分3组：海藻糖组、胎牛血清+10%DMSO组、生理盐水组。于–196 ℃下冻存3个月后，用HE染色、葡萄糖转移法、肌酸激酶法检测脂肪细胞存活情况。结果：海藻糖组及胎牛血清+10%DMSO组脂肪细胞活性均高于生理盐水组(P0.05)；海藻糖组与胎牛血清+10%DMSO组脂肪细胞活性差异无统计学意义(P0.05)。结论：海藻糖作为冻存保护剂能明显保持冷冻脂肪细胞活性，脂肪组织冷冻保存3个月后可用于临床移植。.

Three-dimensional (3D) bioprinting provides an advanced technology for tissue engineering and regenerative medicine because of its ability to produce the models or organs with higher precision and more suitable for human body. It has been successfully used to produce a variety of cartilage scaffold materials. In addition, 3D bioprinter can directly to print tissue and organs with live chondrocytes. In conclusion, 3D bioprinting may have broad prospect for cartilage regeneration and reconstruction in tissue engineering.3D生物打印是3D打印技术在生物医学领域中的交叉应用，能够制作出高精度的更为适合人体的模型和组织器官，利用3D生物打印成功制作出多种软骨细胞生物支架材料，而且能够直接携带软骨细胞进行3D生物打印。3D生物打印为组织工程和再生医学提供了更加先进的手段，为组织工程软骨再生与重建带来了广阔的前景。.

To present a method for quantifying the anastomosis between two vessels based on angiograpy and provide a theoretical basis for vascular study in skin flap.Ten bilateral skin flaps of 20 cm x 20 cm based on deep iliac circumflex vessels were elevated from the abdominal wall including musculus rectus abdominis and deep superior epigastric vessels in 5 minipigs which were 100-115 cm in length and 25-35 kg in weight. One side was inserted an expander under the panniculus carnosus and was expanded regularly with 30-40 ml isotonic Na chloride injection (expanded group) and the other was unexpanded group which were without any treatment. A X-ray image of the flap vessles was obtained after a gelatin-lead oxide mixture was carotid arterially injected and 24 hours of cryopreservation of the body. Three parallel lines with equal interval perpendicular to long axis of the two vessels were designed at the communication area. Vessel anastomosis quantifying was determined by counting the number of marks derived from the intersections of the lines and the vessels and statistical analysis was carried out.The mark of intersection in expanded group (81.20+/-10.33) was more than that in unexpanded group (22.40+/-5.41), showing significant difference (P0.01).The method for quantifying vessel anastomosis in skin flap is simple, reliable, and easy-to-perform. The principles of this procedure may also be applied to other experimental and clinical studies.