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The sleeve grouting connection is the most common form of vertical connection for prefabricated shear walls. However, during construction, this type of connection is prone to defects such as insufficient anchorage length of reinforcement, deviation of reinforcement, and insufficient amount of sleeve grouting, which significantly impact the integrity and seismic performance of the prefabricated shear wall structure. The finite element analysis of the prefabricated shear wall with sleeve grouting connection is still based on solid element modeling. This method has the disadvantages of complex models and low computational efficiency. In this paper, a simplified modeling method for prefabricated shear walls considering sleeve grouting defects was proposed to address this issue. Firstly, the equivalent constitutive relationship of the sleeve grouting defect connector was constructed based on the uniaxial tensile test of the existing sleeve grouting defect connector, and the T3D2 element was used to simulate the sleeve grouting connector. Then, the mechanical behavior of the horizontal joint between the shear wall and the foundation beam was simulated by the cohesive force model, and the prefabricated shear wall models with sleeve grouting defects were established. The accuracy of the simplified modeling method was verified by comparing the experimental results and numerical simulation results of the seismic performance of the prefabricated shear wall with sleeve grouting defects. The results showed that the hysteresis curve, skeleton curve, and failure mode of the numerical simulation were in good agreement with the test results. However, the stiffness of the concrete degenerated rapidly due to the apparent development of plastic-damaged concrete, which made the falling section of the hysteresis curve of the numerical simulation different from that of the test. The proposed simplified modeling method can be further applied to the performance study of prefabricated shear walls with sleeve grouting defects and can provide a reference for structural performance evaluation, design optimization, and construction quality control to a certain extent.

ABSTRACTTransient receptor potential (TRP) channels are evolutionarily conserved integral membrane proteins. They serve as crucial mediators of sensory signals and have profound effects on cellular functions. However, many functions of the TRP family remain to be explored. In this study, the biological functions of three potential TRP channels, TrpA, TrpB, and TrpC, in Aspergillus nidulans have been verified. Green fluorescent protein labeling indicated that these TRP channels have distinct localization patterns. TrpA was primarily located at hyphal tips, TrpB showed a localization pattern resembling the Golgi apparatus, and TrpC displayed a possible ER-like localization pattern. Findings suggested that these TRP channels may be involved in different physiological processes. Deletion or reduced expression of TrpA resulted in severe growth defects and abolished conidiation. Moreover, TrpA may have multiple roles during fungal development, as TrpA mutants caused significant changes in gene expression. The lack of TrpB resulted in colony defects in a calcium-dependent manner, especially under high-temperature conditions. These defects in ΔtrpB can be largely rescued by adding calcium, indicating that TrpB is required in low-calcium conditions. The deletion of TrpC did not exhibit any detectable phenotypes under tested cultural conditions. However, when major calcium channels MidA/CchA or PmrA were absent, the lack of TrpC further exacerbated the colony defects, implying that TrpC serves as a supplementary function. In conclusion, TRP channels are involved in a wide range of physiological processes and findings provide a valuable reference for investigating the functions of TRP proteins in other fungal or eukaryotic species.IMPORTANCETransient receptor potential (TRP) ion channels are evolutionarily conserved integral membrane proteins with non-selective ion permeability, and they are widely distributed in mammals and single-cell yeast and serve as crucial mediators of sensory signals. However, the relevant information concerning TRP channels in Aspergillus nidulans remains inadequately understood. In this study, by gene deletion, green fluorescent protein tagging, and cytosolic Ca2+ transient monitoring techniques, the biological functions of three potential TRP channels (TrpA, TrpB, and TrpC) have been explored for which they play distinct and multiple roles in hyphal growth, conidiation, responsiveness to external stress, and regulation of intracellular Ca2+ homeostasis. The findings of this study on the functions of potential TRP channels in A. nidulans may serve as a valuable reference for understanding the roles of TRP homologs in industrial or medical strains of Aspergillus, as well as in other filamentous fungi.

Mitochondria, as the core metabolic organelles, play a crucial role in aerobic respiration/biosynthesis in fungi. Numerous studies have demonstrated a close relationship between mitochondria and Candida albicans virulence and drug resistance. Here, we report an octapeptide-aminopeptidase located in the mitochondrial matrix named Oct1p. Its homolog in the model fungus Saccharomyces cerevisiae is one of the key proteins in maintaining mitochondrial respiration and protein stability. In this study, we utilized evolutionary tree analysis, gene knockout experiments, mitochondrial function detection, and other methods to demonstrate the impact of Oct1p on the mitochondrial function of C. albicans. Furthermore, through transcriptome analysis, real-time quantitative PCR, and morphological observation, we discovered that the absence of Oct1p results in functional abnormalities in C. albicans, affecting hyphal growth, cell adhesion, and biofilm formation. Finally, the in vivo results of the infection of Galleria mellonella larvae and vulvovaginal candidiasis in mice indicate that the loss of Oct1p led to the decreased virulence of C. albicans. In conclusion, this study provides a solid theoretical foundation for treating Candida diseases, developing new targeted drugs, and serves as a valuable reference for investigating the connection between mitochondria and virulence in other pathogenic fungi.

Generative adversarial network (GAN) can generate diverse and high-resolution images for data augmentation. However, when GAN is applied to the synthetic aperture radar (SAR) dataset, the generated categories are not of the same quality. The unrealistic category will affect the performance of the subsequent automatic target recognition (ATR). To overcome the problem, we propose a reinforced constraint filtering with compensation afterwards GAN (RCFCA-GAN) algorithm to generate SAR images. The proposed algorithm includes two stages. We focus on improving the quality of easily generated categories in Stage 1. Then, we record the categories that are hard to generate and compensate by using traditional augmentation methods in Stage 2. Thus, the overall quality of the generated images is improved. We conduct experiments on the moving and stationary target acquisition and recognition (MSTAR) dataset. Recognition accuracy and Fréchet inception distance (FID) acquired by the proposed algorithm indicate its effectiveness.

Abstract Background A symptomatic postoperative pseudocyst (PP) is a cystic lesion that is formed in the operation area of the intervertebral disc, leading to worse symptoms. Some minority patients who developed PP experienced rapidly aggravating symptoms and could not be treated by any kind of conservative treatment. However, no clinical studies have evaluated the clinical characteristics and surgical strategies of symptomatic PP requiring a revision surgery after full-endoscopic lumbar discectomy (FELD). This study aimed to demonstrate the clinical characteristics and surgical strategies of symptomatic PP requiring a revision surgery after FELD. Methods We retrospectively analyzed the data of patients who received FELD revision surgeries due to symptomatic PP formation between January 2016 and December 2021. Common characteristics, time intervals of symptom recurrence and revision surgery, strategies for conservative treatment and revision surgery, operative time, imaging characteristics, numeric rating scale (NRS) score, Oswestry disability index (ODI) and overall outcome rating based on modified MacNab criteria were analyzed. Results Fourteen patients (males = 10, females = 4), with a mean age of 24.4 years, were enrolled. The mean time intervals of symptom recurrence and revision surgery were 43.5 and 18.9 days respectively. While the patients were conservatively managed with analgesics and physical therapy, pain persisted or progressively worsened. In comparison to the initial herniated disc, the PP was larger in 11 cases, and up- or down-migrated in four cases. The PP location included the lateral recess (n = 12), foraminal (n = 1), and centrolateral (n = 1) zones. One of the two cases treated by percutaneous aspiration (PA) was eventually treated by FELD as pain was not relieved. Follow-ups revealed an improved mean NRS score from 7.1 to 1.4, mean ODI from 68.6 to 7.9% and promising overall surgical outcomes. Conclusions The progressively severe pain experienced due to PP might be a result of its enlargement or migration to the lateral recess and foraminal zones. As complete removal of capsule is the goal, we recommend FELD instead of PA.

Characterizing the molecular mechanism through which different carbon sources affect the denitrification process would provide a basis for the proper selection of carbon sources, thus avoiding excessive carbon source dosing and secondary pollution while also improving denitrification efficiency. Here, we selected Thauera linaloolentis as a model organism of denitrification, whose genomic information was elucidated by draft genome sequencing and KEGG annotations, to investigate the growth kinetics, denitrification performances and characteristics of metabolic pathways under diverse carbon source conditions. We reconstructed a metabolic network of Thauera linaloolentis based on genomic analysis to help develop a systematic method of researching electron pathways. Our findings indicated that carbon sources with simple metabolic pathways (e.g., ethanol and sodium acetate) promoted the reproduction of Thauera linaloolentis, and its maximum growth density reached OD600 = 0.36 and maximum specific growth rate reached 0.145 h−1. These carbon sources also accelerated the denitrification process without the accumulation of intermediates. Nitrate could be reduced completely under any carbon source condition; but in the “glucose group”, the maximum accumulation of nitrite was 117.00 mg/L (1.51 times more than that in the “ethanol group”, which was 77.41 mg/L), the maximum accumulation of nitric oxide was 363.02 μg/L (7.35 times more than that in the “ethanol group”, which was 49.40 μg/L), and the maximum accumulation of nitrous oxide was 22.58 mg/L (26.56 times more than that in the “ethanol group”, which was 0.85 mg/L). Molecular biological analyses demonstrated that diverse types of carbon sources directly induced different carbon metabolic activities, resulting in variations in electron generation efficiency. Furthermore, the activities of the electron transport system were positively correlated with different carbon metabolic activities. Finally, these differences were reflected in the phenomenon of electronic competition between denitrifying reductases. Thus we concluded that this was the main molecular mechanism through which the carbon source type affected the denitrification process. In brief, carbon sources with simple metabolic pathways induced higher efficiency of electron generation, transfer, and competition, which promoted rapid proliferation and complete denitrification; otherwise Thauera linaloolentis would grow slowly and intermediate products would accumulate seriously. Our study established a method to evaluate and optimize carbon source utilization efficiency based on confirmed molecular mechanisms.

Abstract Mitochondrial mass imbalance is one of the key causes of cardiovascular dysfunction after hypoxia. The activation of dynamin-related protein 1 (Drp1), as well as its mitochondrial translocation, play important roles in the changes of both mitochondrial morphology and mitochondrial functions after hypoxia. However, in addition to mediating mitochondrial fission, whether Drp1 has other regulatory roles in mitochondrial homeostasis after mitochondrial translocation is unknown. In this study, we performed a series of interaction and colocalization assays and found that, after mitochondrial translocation, Drp1 may promote the excessive opening of the mitochondrial permeability transition pore (mPTP) after hypoxia. Firstly, mitochondrial Drp1 maximumly recognizes mPTP channels by binding Bcl-2-associated X protein (BAX) and a phosphate carrier protein (PiC) in the mPTP. Then, leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2) is recruited, whose kinase activity is inhibited by direct binding with mitochondrial Drp1 after hypoxia. Subsequently, the mPTP-related protein hexokinase 2 (HK2) is inactivated at Thr-473 and dissociates from the mitochondrial membrane, ultimately causing structural disruption and overopening of mPTP, which aggravates mitochondrial and cellular dysfunction after hypoxia. Thus, our study interprets the dual direct regulation of mitochondrial Drp1 on mitochondrial morphology and functions after hypoxia and proposes a new mitochondrial fission-independent mechanism for the role of Drp1 after its translocation in hypoxic injury.

Abstract Background It is well known that sepsis is a prevalent severe disease caused by infection and the treatment strategies are limited. Recently pericyte-derived microvesicles (PMVs) were confirmed to be therapeutic in many diseases, whether PMVs can protect vascular endothelial cell (VEC) injury is unknown. Methods Pericytes were extracted from the retina of newly weaned rats, and PMVs were collected after starvation and characterized by flow-cytometry and transmission electron microscopy. First, the effect of PMVs on pulmonary vascular function in septic rats was measured via intravenous administration with HE staining, immunofluorescence, and Elisa analysis. Then, PMVs were co-incubated with VECs in the presence of lipopolysaccharide (LPS), and observed the protective effect of PMVs on VECs. Next, the proteomic analysis and further Gene Ontology (GO) enrichment analysis were performed to analyze the therapeutic mechanism of PMVs, and the angiogenesis-related protein CTGF was highly expressed in PMVs. Finally, by CTGF upregulation and downregulation in PMV, the role of PMV-carried CTGF was investigated. Results PMVs restored the proliferation and angiogenesis ability of pulmonary VECs, and alleviated pulmonary vascular leakage in septic rats and LPS-stimulated VECs. Further study showed that PMVs delivered CTGF to VECs, and subsequently activated ERK1/2, and increased the phosphorylation of STAT3, thereby improving the function of VECs. The further study found CD44 mediated the absorption and internalization of PMVs to VECs, the anti-CD44 antibody inhibited the protective effect of PMVs. Conclusions PMVs may delivery CTGF to VECs, and promote the proliferation and angiogenesis ability by activating the CTGF-ERK1/2-STAT3 axis, thereby protecting pulmonary vascular function in sepsis. The therapeutic effect of PMVs was highly related to CD44-mediated absorption. Video Abstract

For buildings that meet the requirements of current seismic design codes, damage to nonstructural components and the internal objects of buildings often become the main source of the seismic economic losses of these buildings. However, the current specifications only consider the safety of ‘no collapse under strong earthquake’ and do not consider ‘functional recoverability’. In this paper, a six-story frame building was taken as an example. Four joint performance limit states were proposed, as per FEMA 273, to establish a two-dimensional probabilistic seismic demand model that considers parameter correlations. The limit state function was established, and the two-dimensional seismic vulnerability curve was calculated. The seismic intensity–economic loss curve and the annual average economic loss established by one-dimensional and two-dimensional seismic vulnerability curves were compared. The results showed that the seismic performance of the structure was lower than expected when using only a one-dimensional seismic vulnerability curve. However, the situation was more serious under high-intensity earthquake and high-performance levels.

Gas fuel direct injection (DI) technology can improve the control precision of the in-cylinder mixing and combustion process and effectively avoid volumetric efficiency reduction in a compressed natural gas (CNG) engine, which has been a tendency. However, compared with the port fuel injection (PFI) method, the former’s mixing path and duration are shortened greatly, which often leads to poor mixing uniformity. What is worse, the in-cylinder mixing performance would be seriously affected by engine working conditions, such as engine speed and load. Based on this situation, the fluid mechanics software FLUENT is used in this article, and the computational fluid dynamics (CFD) model of the injection and mixing process in a gas-fueled direct injection engine is established. A quantitative evaluation mechanism of the in-cylinder mixing performance of the CNG engine is proposed to explore the influencing rule of different engine speeds and loads on the mixing process and performance. The results indicate that phase space analysis can accurately reflect the characteristics of the mixture mixing process. The gas fuel mixture rapidly occupies the cylinder volume in the injection stage. During the transition stage, the gas fuel mixture is in a highly transient state. The diffusion stage is characterized by the continuous homogenization of the mixture. The in-cylinder mixing performance is linearly dependent on the engine’s working condition in the phase space.

Abstract Ultralong organic phosphorescence (UOP) crystals have attracted increased attention due to the distinct photophysical property of a long-lived lifetime. However, organic crystals are generally brittle, leading to a serious problem for their application in flexible technology. Herein, we report three types of elastic organic crystals (EOCs) with ultralong phosphorescence via introducing halogen atoms (Cl, Br, I) into π-conjugated phosphorescent molecules. Especially, the crystal containing iodine atoms displayed both excellent elasticity (ε = 3.01%) and high phosphorescent efficiency (Φ Ph = 19.1%) owing to the strong halogen bonds. Taking advantage of its highly efficient UOP and excellent elasticity, we successfully used a DCz4I crystal for anti-counterfeiting application. These findings may provide guidance for the development of elastic crystals with afterglow and expand the scope of potential applications on flexible materials.

Abstract Background Sepsis is a major cause of death in ICU, and intestinal barrier dysfunction is its important complication, while the treatment is limited. Recently, mesenchymal stem cell-derived microvesicles (MMVs) attract much attention as a strategy of cell-free treatment; whether MMVs are therapeutic in sepsis induced-intestinal barrier dysfunction is obscure. Methods In this study, cecal ligation and puncture-induced sepsis rats and lipopolysaccharide-stimulated intestinal epithelial cells to investigate the effect of MMVs on intestinal barrier dysfunction. MMVs were harvested from mesenchymal stem cells and were injected into sepsis rats, and the intestinal barrier function was measured. Afterward, MMVs were incubated with intestinal epithelial cells, and the effect of MMVs on mitochondrial dynamic balance was measured. Then the expression of mfn1, mfn2, OPA1, and PGC-1α in MMVs were measured by western blot. By upregulation and downregulation of mfn2 and PGC-1α, the role of MMVs in mitochondrial dynamic balance was investigated. Finally, the role of MMV-carried mitochondria in mitochondrial dynamic balance was investigated. Results MMVs restored the intestinal barrier function by improving mitochondrial dynamic balance and metabolism of mitochondria. Further study revealed that MMVs delivered mfn2 and PGC-1α to intestinal epithelial cells, and promoted mitochondrial fusion and biogenesis, thereby improving mitochondrial dynamic balance. Furthermore, MMVs delivered functional mitochondria to intestinal epithelial cells and enhanced energy metabolism directly. Conclusion MMVs can deliver mfn2, PGC-1α, and functional mitochondria to intestinal epithelial cells, synergistically improve mitochondrial dynamic balance of target cells after sepsis, and restore the mitochondrial function and intestinal barrier function. The study illustrated that MMVs might be a promising strategy for the treatment of sepsis.

The corrosion and odor in concrete sewers are mainly related to the sulfide production, which is, under certain circumstances, directly proportional to the hydraulic retention time (HRT) of the sewer. To reduce the corrosion and control the odor in concrete sewers, it is necessary to model the production of sulfide in the concrete sewers with different HRTs. However, previous researches were mostly carried out in simulated Perspex-made sewers, and the obtained theoretical formulas based on the Monod equation were impractical because of the complexity. An actual concrete pipe with domestic sewage was employed in this study to obtain a simple but practical model, which can be applied to quantitively describe the sulfide production according to the HRT of the sewer and the chemical oxygen demand (COD) of the sewage. The empirical equation obtained was , the coefficient is a logarithmic function of the HRT, and the sulfide production rate and COD have a power relationship. Based on the data of COD and HRT obtained in the realistic sewer, the production of sulfide in the sewer can be predicted for better maintaining sewers through sulfide control. HIGHLIGHTS An actual concrete pipe was used to evaluate the effect of HRT on the variation of pollutants.; Few degradations of nitrogen and phosphorus in an actual concrete pipe were found with HRT.; The degradation of COD in an actual concrete pipe accorded with the first-order reaction law.; Sulfide production rate was the power function of solute COD, and the coefficient is the logarithmic function of the HRT.;

Abstract Anaerobic ammonium oxidation (anammox) bacteria significantly improve the efficiency and reduce cost of nitrogen removal in wastewater treatment plants. However, their slow growth and vulnerable activity limit the application of anammox technology. In this paper, the enhancement of biotin on the nitrogen removal activity of anammox bacteria in short-term batch experiments was studied. We found that biotin played a significant role in promoting anammox activity within a biotin concentration range of 0.1–1.5 mg/L. At a biotin concentration of 1.0 mg/L, the total nitrogen removal rate (NRR) increased by 112%, extracellular polymeric substance (EPS) secretion and heme production significantly improved, and anammox bacterial biomass increased to maximum levels. Moreover, the predominant genus of anammox bacteria was Candidatus Brocadia.

Abstract Wastewater characterization is the basis for process design and operation optimization of wastewater treatment plants (WWTPs). In this work, a comprehensive study of the respirometry method has been performed to evaluate the biodegradable organic matters of wastewater. First, the optimal initial substrate to biomass ratio (S0/X0) was confirmed. Second, under the optimal S0/X0, the degradation curves of wastewater carbon oxygen demand (COD) components rapidly biodegradable COD (RBCOD) and slowly biodegradable COD (SBCOD) were obtained. Third, the Mann–Kendall test was performed to confirm the time point (t2) when endogenous respiration levels were reached, and the hydrolysis model was used to determine the time point (t1) of the SBCOD degradation stage. Considering the results, an adequate wastewater COD characterization method for RBCOD and SBCOD has been proposed. This study provides strong support to carry out effective and feasible process design, process diagnosis and optimization capability, can help achieve refined and stable operational management of WWTPs.

Natural gas has emerged as one of the preferred alternative fuels for vehicles owing to its advantages of abundant reserves, cleaner combustion and lower cost. At present, the gas supply methods for natural-gas engines are mainly port fuel injection (PFI) and direct injection (DI). The transient injection characteristics of a gas fuel injection device, as the terminal executive component of the PFI or DI mode, will directly affect the key performance of a gas fuel engine. Therefore, gas fuel injection devices have been selected as the research object of this paper, with a focus on the transient injection process. To explore the impacts of valve vibration amplitude, period, frequency and velocity on transient injection characteristics, one transient computational fluid dynamics (CFD) model for gas fuel injection devices was established. The findings thereof demonstrated that there is a linear relationship between the instantaneous mass flow rate and instantaneous lift during the vibration process. However, this relationship is somewhat impacted when the valve speed is high enough. A shorter valve vibration period tends to preclude a shorter period of flow-hysteresis fluctuation. The near-field pressure fluctuation at the throat of an injection device, caused by valve vibration, initiates flow fluctuation.

The PBL (Perfobond Leiste) shear connector has the advantages of high bearing capacity and strong constraint ability; however, the traditional PBL shear connector has strong and weak axis problems, and its stiffness is large, resulting in weak deformation ability. To this end, this paper proposes a new type of flange triple-web shear key and obtains the new shear key’s mechanical properties and failure mechanism through the push-out test. The results show that the failure mode of the new shear key is the deformation of the steel plate on the web and the edge of the opening, which has a high bearing capacity, outstanding deformation ability, and good integrity with concrete, showing obvious semi-rigid characteristics. Through numerical analysis, the effects of flange width, web height, and steel plate thickness on the mechanical properties of shear keys are obtained. Based on the fitting analysis method, the calculation formula of shear key bearing capacity is proposed. Finally, the horizontal seismic performance of the shear key is numerically simulated. It is found that the hysteretic curve of the shear key is full and shows good energy dissipation capacity.

In recent decades, research on alternative fuel engines is becoming more and more popular. Compressed natural gas (CNG) has the advantages of abundant reserves and a lower cost. It can reduce vehicle emissions relatively quickly and has little impact on the entire transportation infrastructure. As the fourth generation of a gas fuel supply method, gas fuel direct injection (DI) technology can effectively avoid volumetric efficiency reduction and power reduction problems of the port fuel injection (PFI) method. However, the former’s mixing path and duration are shortened greatly, which often leads to poor mixing uniformity. In order to improve the in-cylinder mixing uniformity, the in-cylinder mixing process of the CNG-fueled engine is taken as the research object in this study. The computational fluid dynamics (CFDs) models of the mixing process for the PFI and DI modes are established, and their mixing uniformities are compared. Besides, based on the authors’ previous research, the influence mechanism of the piston crown shape and fuel injection angle on the mixing process of the CNG DI engine is explored. The results show that the probability distribution frequency (PDF) of the best mixture concentration region (BMCR) is as high as 72% for the PFI mode, which is much higher than for the DI mode. The shorter jet impingement distance of the flat top piston leads to higher turbulent kinetic energy (TKE) intensity, and the in-cylinder mixing uniformity will be improved. When gas fuel is injected into an area with a higher in-cylinder TKE, the average in-cylinder TKE will be higher, and the in-cylinder mixture will be more homogeneous.

Activated sludge processes with an ultra-short sludge retention time (ultra-short-SRT) are considered to have potential for energy and resource recovery from wastewater. The present study focused on the sludge characteristics, system performance and microbial kinetics in ultra-short-SRT activated sludge (USSAS) processes using typical domestic wastewater (SRT = 0.5, 1, 2, 3 and 4 d). The results showed that compared with the sludge in conventional activated sludge (CAS) processes, the sludge structure in USSAS system was looser (fractal dimension, D2P, 1.19–1.33), the boundary was rougher (pore boundary fractal dimension, DB, 1.44–1.59), the sludge concentration was lower, and the sludge volume index (SVI) was higher; bacteria such as Thiothrix and Trichococcus that cause sludge bulking, which poses an operation risk, were extensively detected, especially at SRTs of 0.5 d and 1.0 d. The performance in terms of total chemical oxygen demand (tCOD) and phosphorus removal increased with increasing SRT, and the highest removal rate (approximately 85% for tCOD and 90% for phosphorus) was observed when the SRT was 4 d. Both bioconversion and biosorption were responsible for the C/P separation, and their roles were different for different types of organic matter and phosphorus under different SRT conditions. The proportion of phosphate-accumulating organisms (PAO) reached 2.4% when the SRT was 3 d, resulting in highly effective biological phosphorus removal. The values of microbial kinetic parameters such as YH and KdH in USSAS systems were higher than those in CAS systems, indicating faster microbial community renewal. This study was helpful for understanding the characteristics of USSAS process.

Transient Receptor Potential (TRP) proteins constitute a superfamily that encodes transmembrane ion channels with highly diverse permeation and gating properties. Filamentous fungi possess putative TRP channel-encoded genes, but their functions remain elusive. Here, we report that a putative TRP-like calcium channel, trpR, in the filamentous fungus Aspergillus nidulans, performs important roles in conidiation and in adapting to cell wall disruption reagents in a high temperature-induced defect-dependent manner, especially under a calcium-limited culture condition. The genetic and functional relationship between TrpR and the previously identified high-affinity calcium channels CchA/MidA indicates that TrpR has an opposite response to CchA/MidA when reacting to cell wall disruption reagents and in regulating calcium transients. However, a considerable addition of calcium can rescue all the defects that occur in TrpR and CchA/MidA, meaning that calcium is able to bypass the necessary requirement. Nevertheless, the colocalization at the membrane of the Golgi for TrpR and the P-type Golgi Ca2+ ATPase PmrA suggests two channels that may work as ion transporters, transferring Ca2+ from the cytosol into the Golgi apparatus and maintaining cellular calcium homeostasis. Therefore, combined with data for the trpR deletion mutant revealing abnormal cell wall structures, TrpR works as a Golgi membrane calcium ion channel that involves cell wall integration.