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Since the elucidation of the double helix structure of DNA molecule and its role of coding for genetic information, rapid progress of biomedical science including the sequencing of macromolecules in the living system has been witnessed. The technique has allowed the study field to evolve from single genes to genome in species. With demand of deposit and analysis of data obtained, a new science bioinformatics has been formed by integration of computer science and biology. Bioinformatics has found wide application in research of different disciplinary in medical science. To facilitate the grasp of its basic theory and practical application by the postgraduate students at the medical school, regular reading of relevant publications has been proposed as an approach to improve the quality of teaching, and its outcome has been evaluated by comparing the scores earned from the identical test papers. The students acquired the designed intervention had higher score in average statistically with p

The present paper focuses on the semiotics of historization, that is, of “narration as history” and provides a meta-analysis of the semiotic modeling of Confucius as a case in point. I argue that the personage who has come to be known as Confucius has existed and can only exist, without exception, in multiple forms of sociocultural representation and interpretation that are natural results of distinct modeling processes. These forms compose the immediate realities of Confucius for us and the very foundation upon which any further discussions about Confucius, his disciples, and their thoughts and lives are even possible in the first place. This treatment reminds us of how easily we can fall victim to the objective illusion and the confusion of understanding and axiology that Mieke Bal has rightly cautioned against. Moreover, the paper is by no means intended to be a revisionist or nihilistic denial of the historicity of Confucius, but rather an attempt to underscore the central and fundamental role that modeling systems about historical figures play in historization as human sociocultural and cognitive practice. In this sense, the case of Confucius serves as an instance, a specimen, a sign, which is extensible to any other historical figures.

Starting with a critique of so-called intercultural communication, the present paper contests and challenges the prevalent and dominant essentialist views of “culture”. It is exposed that these views have a detrimental underlying logic that is both destructive and self-destructive. Instead, the paper proposes a radically new idea of culture, a minimalist approach supported by insights gleaned from contemporary semiotic inquiry. In this approach, culture is defined as a biological instinct to acquire information through modeling, that is, learning by models. This instinct is at work, or is realized, in specific acts of such modeling, resulting in cultural practices and cultural artifacts. In the case of humanity, a cultural practice is anything a human does that can be modeled by another human and a cultural artifact is any object that humans make and can model. The paper argues it is imperative to keep in mind that when we deal with the “intercultural”, we are only dealing with concrete yet different cultural practices or cultural artifacts. This is an effective way to completely refute essentialism. In a sense, the paper is meant to be a wake-up call, instead of a fighting talk. Its main objective is not to negate or obliterate the field of “intercultural communication”, among others, but rather to save them from themselves—a true and worthy field of “intercultural communication” is a field against essentialism, instead of an accessory to essentialism, whether the commission is “before the fact” or “after the fact”.

Defect sites on oxide semiconductors play a crucial role in promoting photocatalytiperformance and modulating the bandgap structure of photocatalysts. However, the role of interfacial coordinatively unsaturated defect sites between metal and oxide in photocatalysis is still under debate. So, we designed an experiment to probe the role of interfacial coordinatively unsaturated defect sites. In this work, a series of Ti/TiO2 photocatalysts with varying concentrations of interfacial Ti3+ sites were prepared through an epitaxial growth method under hydrothermal conditions. Through experimental and computational investigations, the roles of interfacial defect sites were discussed in detail. On the one hand, the interfacial coordinatively unsaturated Ti3+ sites could act as visible-light-responsive sites in photocatalytic reactions due to the overlap and hybridization of multiple electronic orbitals. On the other hand, the Ti/TiO2 interface exhibited a certain degree of metallic character near the Fermi level because of the partial delocalization and redistribution of electrons, facilitating the charge migration and separation across the metal-oxide interface. Consequently, the obtained Ti/TiO2 catalysts showed notably enhanced charge transfer efficiency and visible light photocatalytic activity compared to their pristine counterparts. This work may provide a new perspective to interfacial defect engineering in classic metal/oxide heterojunction photocatalysts and figure a more precise direction to synthesize higher effective photocatalysts for environmental governance.

This paper affords a Lotmanian cultural semiotic analysis of the inner workings of ritual embodying the mechanism of cultural memory. In this intersectional study, we propose treating ritual as an integral semiotic system in which the community follows a prescribed collective process to create religious or social meanings and to regulate the mechanism of cultural memory through concrete symbols in the forms of behavior, speech, gestures, objects, spatial structures, and so on. Three semiotic properties of ritual in relation to cultural memory are identified, namely, continuity, concreteness, and integrity, which are jointly responsible for the efficacy of ritual in cultural memory making, or to be specific, in the preservation, retrieval, and even reproduction of cultural memory. With these properties, ritual helps construct and maintain socio-cultural order and group identity in the community, by repeating itself and thus creating a sense of continuity through the preservation and retrieval of cultural memory. The key components in ritual are its diverse and polysemous symbols, which are seldom confined to a specific context, although they are indeed subject to the dominant symbol and the dominant meaning in a ritual when necessary. With an extraordinary degree of autonomy and not bound to any fixed context, ritual symbols can enter a ritual situation as its components but retain the freedom of leaving the ritual context at any time, like an unchained “spectator,” and permeating multiple contexts as self-contained units. It is precisely through these transferrable ritual symbols that the fragments of cultural memory are randomly dispersed in the semiosphere and carried to unexpected socio-cultural contexts, bridging the past, the present, and the future and creating new cultural memory.

Summary The immature characteristics and metabolic phenotypes of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) restrict their applications for disease modeling, drug discovery, and cell-based therapy. Leveraging on the metabolic shifts from glycolysis to fatty acid oxidation as CMs mature, a human hexokinase1-GFP metabolic reporter cell line (H7 HK1-GFP) was generated to facilitate the isolation of fetal or more matured hPSC-CMs. RNA sequencing of fetal versus more matured CMs uncovered a potential role of interferon-signaling pathway in regulating CM maturation. Indeed, IFN-γ-treated CMs resulted in an upregulation of the JAK-STAT pathway, which was found to be associated with increased expression of CM maturation genes, shift from MYH6 to MYH7 expression, and improved sarcomeric structure. Functionally, IFN-γ-treated CMs exhibited a more matured electrophysiological profile, such as increased calcium dynamics and action potential upstroke velocity, demonstrated through calcium imaging and MEA. Expectedly, the functional improvements were nullified with a JAK-STAT inhibitor, ruxolitinib., Highlights • RNA-seq revealed upregulation of IFN-signaling pathways during CM maturation • IFN-γ-treated PSC-derived fetal CMs display increased MYH7:MYH6 ratio • IFN-γ-treated PSC-derived fetal CMs exhibited improved electrophysiological profile, In this article, Soh and colleagues utilize a metabolic reporter to identify a novel role of JAK-STAT signaling in hPSC-CM maturation. IFN-γ treatment activates the JAK-STAT pathway and upregulates STAT1 expression in hPSC-derived fetal CMs, thereby enhancing the expression of CM maturation genes including MYH7, which subsequently resulted in improved sarcomeric structure, and a relatively more matured electrophysiological profile in vitro.

Background ZMYND11 is a reader of histone proteins and plays an important inhibition role in tumor. There is growing evidence to support its importance, but without a pan-cancer analysis, Based on multiple databases, we conducted a comprehensive analysis. Methods Tumor data from both The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were a clear relationship observed gene expression of ZMYND11, state of survival, induction of DNA methylation, alterations in genes, contribution to the parameter of protein phosphorylation, lymphocyte immune infiltration, and correlative cellular pathways. Results ZMYND11 was under-expressed in the great majority of carcinomas. High expression group of ZMYND11 offers a more favorable prognosis in KIRC, LGG, MESO, LUAD,PRAD,READ,THCA,BRCA, SARC and UVM. Alterations in N304Kfs*33, K345Rfs*43 and N271Tfs*18 occur in structurally important domains of ZMYND11 and was found in prostate, colorectal and breast cancers.In most tumour tissues, ZMYND11 promoter DNA methylation was lower than in normal tissues, and promoter methylation was largely negatively correlated with mRNA expression levels. We observed low levels of phosphorylation at locus 349 protein in KIRC were identified with low total ZNYND11 protein expression. High phosphorylation levels at locus 393 protein in breast cancer with high total ZNYND11 protein expression. ZMYND11 gene expression in human tumors is mostly reflected by positively relation to immune cell infiltration while it is confirmed by positive association with CD8 + T cell. There was a differential expression of ZMYND11 in different immune subtypes. The gene enrichment SOSO2, PJA2 and KIAA1109 may be molecules interacting with ZMYND11.KEGG and GO analysis involving in RNAase II promoter regulation, positive and negative regulation of the RNAase II promoter, positive and negative regulation of DNA transcription, chromatin modification. Conclusions This is a comprehensive pan-cancer analysis of the ZMYND11. It describes the role of ZMYND11 in tumour. and highlights a potential target for ZMYND11 in tumour.

This paper provides an integrative and updated view of modeling in semiotics. It postulates that the essence of modeling is supersession. In any act or instance of modeling, the model supersedes and is brought to the front for salience, accessibility, and operability, whereas at the same time the modeled recedes and exists in the background, inaccessible and inoperable. The paper goes on to differentiate between two major types of modeling, the underlying “existential modeling,” functioning as the fundamental scaffold and the genuine foundation of all other types of modeling as we know them, and the overlaying “semiotic modeling,” designating the process of creation and use of “forms of meaning,” a process that underlies both cognition and communication. By focusing on semiotic modeling, the paper features an unconventional view that casts a new light on the relation between a model and a sign and thus the relation between semiotic modeling and semiosis. Endorsing an embodied approach to meaning-making as semiotic modeling, the paper finds it important to stress the appropriateness and necessity of understanding the term “model” as a verb rather than as a noun, in that modeling is never static and should be properly regarded in terms of embodied action.

The present issue of Chinese Semiotic Studies is published in memory of Thomas A. Sebeok. Sebeok was not only a master semiotician, but more importantly a grand artist in semiotics. As one of the most important contemporary figures in semiotics, linguistics, ethnology, and cultural studies, Sebeok made profound contributions to the progress of global semiotics through his distinguished theoretical achievements and promotional activities. His works have proven to be so relevant that they continue to exert a determinative influence and provide directions for the development of semiotics and its many subdivisions, especially biosemiotics, beyond the 20th century. Now, 21 years into the present century, during which semiotic studies around the world have made remarkable progress, it is about time to highlight some specific ways in which his contributions will continue to shape and guide semiotic studies, demonstrating the relevance of these contributions to the 21st century challenges. To this end, this special issue presents some up-to-date and informed studies that explore Sebeok’s contributions to semiotics and their vital implications for fundamental problems relevant to humanity as a semiotic animal in the present day and in the rest of this century and beyond.

This study is based on the practice of cleaner production (CP) audits in more than 300 enterprises. After reviewing 1484 CP options, it is found that 82.5% of the options are directly and indirectly related to management. Moreover, there are common problems such as the poor sustainability of CP audit results. This shows that enterprise management plays a significant role in implementing CP options. Based on these, this study proposes a new concept of cleaner production management (CPM) system, and draws lessons from the modern environmental management theory and method to establish the framework of CPM system. This system combines the theory and method of CP with the management system of enterprises and presents the CPM requirements in the current enterprise management system, which mainly includes CPM responsibilities, CP objectives, CPM indexes, CP energy resources management, CP process control, CP product management, CPM inspections, and CP performance evaluation. The characteristic of the proposed system is to construct a modern information-based CPM system. With highly systematic, concise structure, and easy operability, this system not only simplifies and improves the CP work in enterprises and reduces several tedious tasks such as document preparation, but also enhance the enterprise management efficiency and motivate the willingness of employees to participate in CP. Innovated and developed for CP implementation, the system has been implemented and applied in Chinese enterprises, and the implementation and application have obvious effects on improving production level as well as environmental and economic benefits.

Understanding the mechanisms of plasticity in structural steels is essential for the operation of next-generation fusion reactors. Elemental composition, particularly the amount of Cr present, and irradiation can have separate and synergistic effects on the mechanical properties of ferritic/martensitic steels. The study of ion-irradiated FeCr alloys is useful for gaining a mechanistic understanding of irradiation damage in steels. Previous studies of ion-irradiated FeCr did not clearly distinguish between the nucleation of dislocations to initiate plasticity, and their propagation through the material as plasticity progresses. In this study, Fe3Cr, Fe5Cr, and Fe10Cr were irradiated with 20 MeV Fe$^{3+}$ ions at room temperature to nominal doses of 0.01 dpa and 0.1 dpa. Nanoindentation was carried out with Berkovich and spherical indenter tips to study the nucleation of dislocations and their subsequent propagation. The presence of irradiation-induced defects reduced the theoretical shear stress and barrier for dislocation nucleation. The presence of Cr further enhanced this effect due to increased retention of irradiation defects. However, this combined effect is still small compared to dislocation nucleation from pre-existing sources such as Frank-Read sources and grain boundaries. The yield strength, an indicator of dislocation mobility, of FeCr increased with irradiation damage and Cr. The increased retention of irradiation defects due to the presence of Cr also further increased the yield strength. Reduced work hardening capacity was also observed following irradiation. The synergistic effects of Cr and irradiation damage in FeCr appear to be more important for the propagation of dislocations, rather than their nucleation., 32 pages, 10 figures

Tungsten is a promising candidate material for plasma-facing armour components in future fusion reactors. A key concern is irradiation-induced degradation of its normally excellent thermal transport properties. In this comprehensive study, thermal diffusivity degradation in ion-implanted tungsten and its evolution from room temperature (RT) to 1073 K is considered. Five samples were exposed to 20 MeV self-ions at RT to achieve damage levels ranging from 3.2 x 10(-4) to 3.2 displacements per atom (dpa). Transient grating spectroscopy with insitu heating was then used to study thermal diffusivity evolution as a function of temperature. Using a kinetic theory model, an equivalent point defect density is estimated from the measured thermal diffusivity. The results showed a prominent recovery of thermal diffusivity between 450 K and 650 K, which coincides with the onset of mono-vacancy mobility. After 1073 K annealing samples with initial damage of 3.2 x 10(-3) dpa or less recover close to the pristine value of thermal diffusivity. For doses of 3.2 x 10(-2) dpa or higher, on the other hand, a residual reduction in thermal diffusivity remains even after 1073 K annealing. Transmission electron microscopy reveals that this is associated with extended, irradiation-induced dislocation structures that are retained after annealing. A sensitivity analysis shows that thermal diffusivity provides an efficient tool for assessing total defect content in tungsten up to 10 0 0 K. (c) 2022 The Author(s). Published by Elsevier Ltd on behalf of Acta Materialia Inc.

Increased intestinal permeability is seen in a variety of inflammatory conditions such as enteric infections and inflammatory bowel disease. Because barrier function can provide a key biomarker of disease severity, it often is assayed in animal models. A common methodology involves gavaging mice with fluorescein isothiocyanate-conjugated dextran (FITC-D), followed by cardiac puncture to assay plasma fluorescence on a spectrophotometer. Although the FITC-D method is relatively simple, its sensitivity is limited and enables only a single measurement because the test requires killing the subject. Herein, we describe a novel flow cytometry-based method of intestinal permeability measurement based on detection of orally gavaged ovalbumin (OVA) that leaks out of the gut. Our approach uses minute blood volumes collected from the tail vein, permitting repeated testing of the same subject at multiple time points. By comparing this assay against the gold standard FITC-D method, we show the expanded utility of our OVA assay in measuring intestinal permeability.We directly compared our OVA assay against the FITC-D assay by co-administering both probes orally to the same animals and subsequently using their respective methodologies to measure intestinal permeability by detecting probe levels in the plasma. Permeability was assessed in mice genetically deficient in intestinal mucus production or glycosylation. In addition, wild-type mice undergoing dextran sodium sulfate-induced colitis or infected by the enteric bacterial pathogen Citrobacter rodentium also were tested.The OVA assay showed very high efficacy in all animal models of intestinal barrier dysfunction tested. Besides identifying intestinal barrier dysfunction in mice with impaired mucin glycosylation, the assay also allowed for repeated tracking of intestinal permeability within the same animal over time, providing data that cannot be easily acquired with other currently applied methods.The OVA assay is a highly sensitive and effective method of measuring intestinal permeability in mouse models of barrier dysfunction and experimental colitis.

Ion-irradiated FeCr alloys are useful for understanding and predicting neutron damage in the structural steels of future nuclear reactors. Previous studies have largely focused on the structure of irradiation induced defects, probed by transmission electron microscopy (TEM), as well as changes in mechanical properties. Across these studies, a wide range of irradiation conditions has been employed on samples with different processing histories, which complicates the analysis of the relationship between defect structures and material properties. Furthermore, key properties, such as irradiation-induced changes in thermal transport and lattice strain, are little explored. Here we present a systematic study of Fe3Cr, Fe5Cr and Fe10Cr binary alloys implanted with 20 MeV Fe3+ ions to nominal doses of 0.01 dpa and 0.1 dpa at room temperature. Nanoindentation, transient grating spectroscopy (TGS) and X-ray micro-beam Laue diffraction were used to study the changes in hardness, thermal diffusivity and strain in the material as a function of damage and Cr content. Our results suggest that Cr leads to an increased retention of irradiation-induced defects, causing substantial changes in hardness and lattice strain. However, thermal diffusivity varies little with increasing damage and instead degrades significantly with increasing Cr content in the material. We find significant lattice strains even in samples exposed to a nominal displacement damage of 0.01 dpa. The defect density predicted from the lattice strain measurements is significantly higher than that observed in previous TEM studies, suggesting that TEM may not fully capture the irradiation-induced defect population. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

The development of denitrifying polyphosphate accumulating organisms (DPAOs) presents a strategy to carbon competition between denitrifying bacteria and phosphorus removing bacteria. However, low temperature inhibits the rate of enzyme-catalyzed and substrate diffusion during denitrifying phosphorus removal (DPR). Therefore, the present study assessed the addition of NQS (100 μmol/L) for enhancing the removal of TP and TN in DPR reactors operated at alternating anaerobic and anoxic phases and different influent phosphate concentrations. The results showed that the removal efficiency of TP and TN in NQS-DPR system at 10 °C were 99.9% and 42.0%, respectively, which were 2.1 and 2.0 times higher than that of DPR system. Adding NQS significantly alleviated the increase of pH under anoxic condition and decreased the ORP value of the reactor, which in turn enhanced the PHAs accumulation process. The determination of functional genes (nirK, narG and phoD) showed that Dechloromonas, Lentimicrobium, and Terrimonas were the dominant functional bacteria in NQS-DPR system at 10 °C with the relative abundance of 3.09%, 2.99% and 2.28%, respectively. This study can provide valuable information for the effects of the addition of the redox mediator on denitrifying phosphorus removal technology.

To achieve sustainable metal-free electron-Fenton, N self-doped biochar air-cathode (BCAC) was prepared by pyrolyzing coffee residues. During the pyrolysis process, the endogenous N transformed from edge-doping to graphite-doping. Particularly, N vacancies started to evolve when the peak temperature exceeded 700 °C. A high Tetracycline removal rate of 70.42% was obtained on the BCAC at the current density of 4 mA cm

This paper affords a critical and historical reappraisal of Bakhtin’s theory of polyphony. It addresses the issue of the subjectivity of interpretation in the reception and formulation of this highly influential theory in literary semiotics. Following a revaluation of three major patterns of interpretation of polyphony that have emerged in the global field of literary theory since 1929, as well as Bakhtin’s shift in emphasis in 1963, we find that Bakhtin’s theorizing of polyphony, based on his seemingly inconsistent interpretation of Dostoevsky’s novels, was defined by his own subjectivity as well. An obvious consequence of such subjective predispositions in both the reception of Bakhtin’s theory and his own treatment of Dostoevsky’s polyphonic novels is that they have instigated a type of perpetuating availability bias in approaching the theory of polyphony. This revelation is key to understanding the wholeness of the theory of polyphony from a diachronic perspective. By tracing the cultural and intellectual sources of Dostoevsky’s polyphonic creation, this paper attempts to reframe and restore the Bakhtinian idea of polyphony to its fullness, which we believe can be encapsulated in one phrase: harmony without uniformity.

In the face of myriad crises in modern societies, semiotic inquiry has many valuable contributions to make. However, the long-standing dominant analytical paradigms in the field have made it exceedingly difficult, if not altogether impossible, to tackle the countless unanalyzable aspects of semiosis in the human condition. What needs to be done in semiotics is to highlight another mode of knowing, synthetic thinking, without excluding the analytical mode. Drawing inspiration and strength from classical Eastern philosophies and aesthetics, notably I Ching and Laozi, as well as classics and advances in global semiotics, the present paper proposes a cultural semiotics of jingshen, understood here as the holistic flux of mind, vitality, and creativity. This route of inquiry seeks cogent coalescence of the two foregoing modes of knowing so as to better inform semiotics in a new age. At the same time, it creates a unique methodology: the fusion of revelatory “embodied cognition” and “cognition via knowledge/ abstraction.” Viewed in this light, the purpose or function of semiotics is not limited to understanding signs and sign relations or uncovering laws governing the evolution of semiosis, but more importantly it embraces the improvement of mental capacity, the expansion of cognitive space, and the liberation of human thinking.

It is well-known that steels always oxidize faster in the environments containing water vapour than in dry oxygen. Due to the difficulties in obtaining necessary experimental scale of observations, the mechanisms responsible for the steam-accelerated oxidation are still unclear. Through a combination of multiscale characterization techniques, the surface oxide film formed on an Fe-17Cr-9Ni stainless steel after exposure to high-temperature steam has been studied in detail. The characterization results obtained in this study reveal that the inner oxide layer actually consists of two phases Fe-Ni austenite and FeCr2O4 oxide, which formed due to internal oxidation. The classic internal oxidation model underestimates the thickness of the inner oxide layer by one order of magnitude. This difference can be explained by the existence of fast diffusion channels in the inner oxide layer. This study provides direct evidence of a high density of nanopores in the oxide phase of the internal oxide layer, which can act as fast-diffusion channels if interconnected, and proposes their mechanisms of formation, a consequence of water dissociation-induced protons promoting the formation, migration, and clustering of both cation and anion vacancies.

Elastic interactions play an important role in controlling irradiation damage evolution, but remain largely unexplored experimentally. Using transmission electron microscopy (TEM) and high-resolution on-axis transmission Kikuchi diffraction (HR-TKD), we correlate the evolution of irradiation-induced damage structures and the associated lattice strains in self-ion irradiated pure tungsten. TEM reveals different dislocation loop structures as a function of sample thickness, suggesting that free surfaces limit the formation of extended defect structures found in thicker samples. HR-TKD strain analysis shows the formation of crystallographically-orientated long-range strain fluctuation above 0.01 dpa and a decrease of total elastic energy above 0.1 dpa.

Furfural and 5-hydroxymethylfurfural (HMF) have been identified as promising bio-platform furans that have a wide range of potential applications as biofuels, bioplastics, and biochemicals. Furfural and HMF are typically synthesized from the substrates of C5 sugars and C6 sugars, respectively. Furfural can also be produced from C6 sugars, which is technically more challenging owing to the higher energy requirement for carbon&ndash, carbon bond cleavage. In this study, the simultaneous production of furfural and HMF from glucose was conducted over different binary catalyst systems of Br&oslash, nsted acids and Lewis acids using &gamma, valerolactone (GVL) as the solvent. A promising performance was achieved by a SnSO4-H2SO4 coupling catalyst, with an optimized furfural yield of 42% and an HMF yield of 34% at 443 K in GVL. In addition, a kinetics study was performed in order to understand the mechanism of the simultaneous formation of furfural and HMF from glucose at different temperatures and GVL/water ratios. The results showed that the ratio of furfural to HMF production rate at different temperatures (433 to 463 K) or GVL/water ratios (90 to 80%) was constant close to 1, suggesting that the production of furfural and HMF might follow similar reaction pathways. Finally, the reaction pathway of glucose conversion to furfural and HMF was proposed based on the experimental and kinetics studies.