114 results on '"Hong-Yu Ma"'
Search Results
2. Comparison of postoperative analgesia and side effects in pediatric laparoscopic surgery with morphine and nalbuphine
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Jia Chen, Cheng-Yu Wang, John Wei Zhong, Yu-Hang Cai, Jianmin Zhang, Fang Wang, Mazhong Zhang, Hongbin Gu, Hong-Yu Ma, Zhen Wang, Junzheng Wu, and Hua-Cheng Liu
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Health sciences ,Medicine ,Medical specialty ,Surgery ,Pharmacology ,Natural sciences ,Science - Abstract
Summary: There is currently no consensus on the optimal perioperative pain management strategy involving specific opioids. This study aims to compare the postoperative analgesia, the associated side effects between nalbuphine and morphine in children undergoing laparoscopic surgery. One hundred ninety children were randomly assigned to nalbuphine (0.2 mg/kg) or morphine (0.2 mg/kg). Nalbuphine’s analgesic effect was non-inferior to morphine, with similar total rescue analgesic consumption during PACU stay (0.03 ± 0.05mg vs. 0.04 ± 0.06 mg, p > 0.05). Nalbuphine group had a lower incidence of respiratory depression (RR ≤ 10/min) (4.8% vs. 38.6%, p
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- 2024
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3. A framework from point clouds to workpieces
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Li-Yong Shen, Meng-Xing Wang, Hong-Yu Ma, Yi-Fei Feng, and Chun-Ming Yuan
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Computer numerical control ,Mesh reconstruction ,Mesh segmentation ,Path planning ,Scallop height ,Drawing. Design. Illustration ,NC1-1940 ,Computer applications to medicine. Medical informatics ,R858-859.7 ,Computer software ,QA76.75-76.765 - Abstract
Abstract Combining computer-aided design and computer numerical control (CNC) with global technical connections have become interesting topics in the manufacturing industry. A framework was implemented that includes point clouds to workpieces and consists of a mesh generation from geometric data, optimal surface segmentation for CNC, and tool path planning with a certified scallop height. The latest methods were introduced into the mesh generation with implicit geometric regularization and total generalized variation. Once the mesh model was obtained, a fast and robust optimal surface segmentation method is provided by establishing a weighted graph and searching for the minimum spanning tree of the graph for extraordinary points. This method is easy to implement, and the number of segmented patches can be controlled while preserving the sharp features of the workpiece. Finally, a contour parallel tool-path with a confined scallop height is generated on each patch based on B-spline fitting. Experimental results show that the proposed framework is effective and robust.
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- 2022
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4. Early diagnosis of intracranial atherosclerotic large vascular occlusion: A prediction model based on DIRECT-MT data
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He Li, Hong-Yu Ma, Lei Zhang, Pei Liu, Yong-Xin Zhang, Xiao-Xi Zhang, Zi-Fu Li, Peng-Fei Xing, Yong-Wei Zhang, Qiang Li, Peng-Fei Yang, and Jian-Min Liu
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intracranial atherosclerosis ,prediction model ,large vascular occlusion ,etiology of ischemic stroke ,DIRECT-MT ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
AimsThis study aimed to build a prediction model to early diagnose intracranial atherosclerosis (ICAS)-related large vascular occlusion (LVO) in acute ischemic stroke patients before digital subtractive angiography.MethodsPatients enrolled in the DIRECT-MT trial (NCT03469206) were included in our secondary analysis and distributed into ICAS-LVO and non-ICAS-LVO groups. We also retrieved demographic data, medical histories, clinical characteristics, and pre-operative imaging data. Hypothesis testing was used to compare data of the two groups, and univariate logistic regression was used to identify the predictors of ICAS-LVO primarily. Then, we used multivariate logistic regression to determine the independent predictors and formulate the prediction model. Model efficacy was estimated by the area under the receiver operating characteristic (ROC) curve (AUC) and diagnostic parameters generated from internal and external validations.ResultsThe subgroup analysis included 45 cases in the ICAS-LVO group and 611 cases in the non-ICAS-LVO group. Variates with p < 0.1 in the comparative analysis were used as inputs in the univariate logistic regression. Next, variates with p < 0.1 in the univariate logistic regression were used as inputs in the multivariate logistic regression. The multivariate logistic regression indicated that the atrial fibrillation history, hypertension and smoking, occlusion located at the proximal M1 and M2, hyperdense artery sign, and clot burden score were related to the diagnosis of ICAS-LVO. Then, we constructed a prediction model based on multivariate logistics regression. The sensitivity and specificity of the model were 84.09 and 74.54% in internal validation and 73.11 and 71.53% in external validation.ConclusionOur current prediction model based on clinical data of patients from the DIRECT-MT trial might be a promising tool for predicting ICAS-LVO.
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- 2022
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5. Prevention of polycystic ovary syndrome and postmenopausal osteoporosis by inhibiting apoptosis with Shenling Baizhu powder compound
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Jing Liang, Ai-li Bao, Hong-yu Ma, Wei Dong, Wei-hua Li, Xi Wu, Han-yu Li, Hai-yan Hou, Ya-qiong Chen, Jia-lin Fu, and Chao Shao
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Shenling Baizhu powder compound ,Aromatic hydrocarbon receptor (AHR) ,Polycystic ovary syndrome (POCS) ,Postmenopausal osteoporosis (PMO) ,Cell apoptosis ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Objective Shenling Baizhu powder (SBP) has been shown to reverse the abnormal expression of the aromatic hydrocarbon receptor (AHR) mediated by air pollution. Our study aimed to understand the main ingredient of SBP and investigate its action mechanism in preventing polycystic ovary syndrome (POCS) and postmenopausal osteoporosis (PMO). Methods The active ingredients of SBP with the highest binding affinity to AHR were screened using a Chinese medicine database, and their binding mechanism was simulated using molecular dynamics simulation (MDS). Rutin was utilized to treat ovarian granulosa cell lines and osteoblast cell lines. The cell lines were treated with a gradient of rutin concentration (0.01 mmol/L, 0.05 mmol/L and 0.1 mmol/L) to find the optimal drug dose. PCR was used to detect AHR and apoptosis-related proteins, and WB to detect the expression of AHR, caspase-3 and cleaved-caspase-3. Finally, the CCK-8 cell proliferation assay detected the proliferation of cells. Results We obtained Rutin through the Chinese medicine database, and dynamics simulation determined its binding sites. Ovarian granulosa cell lines and osteoblast cell lines were treated with Rutin. RT-PCR and western blotting revealed that the expression of apoptosis-associated protein Bcl-2 was elevated, and the expression of AHR, Bax, caspase-3 and PARP were decreased. CCK-8 results showed accelerated proliferation in both cell types. Conclusion Rutin, the main ingredient of SBP compound, works by binding to AHR, which can improve POCS and PMO by inhibiting cell apoptosis and by promoting cell proliferation.
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- 2022
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6. TNFAIP8 Promotes Cisplatin Chemoresistance in Triple-Negative Breast Cancer by Repressing p53-Mediated miR-205-5p Expression
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Hong-Yu Ma, Yang Li, Hui-Zi Yin, Hang Yin, Yuan-Yuan Qu, and Qing-Yong Xu
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TNFAIP8 ,miR-205-5p ,cisplatin tolerance ,triple-negative breast cancer ,TRAF2 ,p53 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Tumor necrosis factor alpha-induced protein 8 (TNFAIP8) is implicated in the tumor progression and prognosis of triple-negative breast cancer (TNBC), but the detailed regulatory mechanism of TNFAIP8 in cisplatin tolerance in TNBC has not yet been investigated. TNFAIP8 was evidently upregulated in TNBC tumor tissues and cell lines. Knocking down TNFAIP8 led to impaired proliferation and elevated apoptosis of TNBC cells upon cisplatin (DDP) treatment. Mechanistic studies revealed that TNFAIP8 repressed the expression of p53 and p53-promoted microRNA (miR)-205-5p; moreover, miR-205-5p targeted multiple genes required for the cell cycle and repressed Akt phosphorylation, which thus inhibited the proliferation of TNBC cells. In addition, silencing of TNFAIP8 led to the upregulation of miR-205-5p and the restraint of the TRAF2-NF-κB pathway, which thus enhanced the suppressive effects of DDP on tumor growth in nude mice. This study revealed that TNFAIP8 was essential in the DDP tolerance formation of TNBC cells by reducing p53-promoted miR-205-5p expression. Thus, targeting TNFAIP8 might become a promising strategy to suppress TNBC progression.
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- 2020
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7. LncRNA H19 promotes triple-negative breast cancer cells invasion and metastasis through the p53/TNFAIP8 pathway
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Yang Li, Hong-Yu Ma, Xiao-Wei Hu, Yuan-Yuan Qu, Xin Wen, Yu Zhang, and Qing-Yong Xu
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lncRNA H19 ,Breast cancer ,Metastasis ,p53 ,TNFAIP8 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Long non-coding RNA H19 (lncRNA H19) has been implicated in tumorigenesis and metastasis of breast cancer through regulating epithelial to mesenchymal transition (EMT); however, the underlying mechanisms remain elusive. Methods LncRNA H19 and TNFAIP8 were identified by qRT-PCR and western blotting. CCK-8 assay, clone formation assay, transwell assay, and flow cytometry assay were performed to determine cell proliferation, migration, invasion and cell cycle of breast cancer respectively. Western blotting and immunohistochemistry (IHC) were utilized to evaluate the protein expression levels of p53, TNFAIP8, and marker proteins of EMT cascades in vivo. Dual luciferase reporter assay and RNA pull down assay were conducted to evaluate the interactions of lncRNA H19, p53 and TNFAIP8. Results The expression of lncRNA H19 and TNFAIP8 was up-regulated in breast cancer tissues and cell lines, especially in triple-negative breast cancer (TNBC). Functionally, knockdown of lncRNA H19 or TNFAIP8 coused the capacities of cell proliferation, migration, and invasion were suppressed, and cell cycle arrest was induced, as well as that the EMT markers were expressed abnormal. Mechanistically, lncRNA H19 antagonized p53 and increased expression of its target gene TNFAIP8 to promote EMT process. Furthermore, silencing of lncRNA H19 or TNFAIP8 also could inhibit tumorigenesis and lymph node metastases of MDA-MB-231 cells in xenograft nude mouse models. Conclusions Our findings provide insight into a novel mechanism of lncRNA H19 in tumorigenesis and metastases of breast cancer and demonstrate H19/p53/TNFAIP8 axis as a promising therapeutic target for breast cancer, especially for TNBC.
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- 2020
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8. Integrated Microbiome–Metabolome Analysis Reveals Stage-Dependent Alterations in Bacterial Degradation of Aromatics in Leptinotarsa decemlineata
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Wei-Nan Kang, Lin Jin, Hong-Yu Ma, and Guo-Qing Li
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Leptinotarsa decemlineata ,stage-specific bacteria ,habitat ,aromatics ,biodegradation ,Physiology ,QP1-981 - Abstract
To avoid potential harm during pupation, the Colorado potato beetle Leptinotarsa decemlineata lives in two different habitats throughout its developmental excursion, with the larva and adult settling on potato plants and the pupa in soil. Potato plants and agricultural soil contain a specific subset of aromatics. In the present study, we intended to determine whether the stage-specific bacterial flora plays a role in the catabolism of aromatics in L. decemlineata. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the operational taxonomic units (OTUs) obtained by sequencing of culture-independent 16S rRNA region enriched a group of bacterial genes involved in the elimination of mono- and polycyclic aromatics at the pupal stage compared with those at the larval and adult periods. Consistently, metabolome analysis revealed that dozens of monoaromatics such as styrene, benzoates, and phenols, polycyclic aromatics, for instance, naphthalene and steroids, were more abundant in the pupal sample. Moreover, a total of seven active pathways were uncovered in the pupal specimen. These ways were associated with the biodegradation of benzoate, 4-methoxybenzoate, fluorobenzoates, styrene, vanillin, benzamide, and naphthalene. In addition, the metabolomic profiles and the catabolism abilities were significantly different in the pupae where their bacteria were removed by a mixture of three antibiotics. Therefore, our data suggested the stage-dependent alterations in bacterial breakdown of aromatics in L. decemlineata.
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- 2021
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9. An integrated study for the utilization of anthraquinone compounds extract 'Heshouwu' In vivo and their comparative metabolism in liver microsomes using UPLC-ESI-Q-TOF/MSn
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Sha Chen, Hong-Yu Ma, Zhe Deng, Jun Zhang, Jin-Tang Cheng, Chang Chen, and An Liu
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Anthraquinones ,liver microsomes ,UPLC-QqQ-MS/MS ,UPLC-Q-TOF/MS ,Medicine (General) ,R5-920 - Abstract
Objective: Anthraquinone (AQ), a major bioactive component of the traditional Chinese medicine HeShouWu, has widespread applications in industry and medicine. The objective of the current study is to explore the differences in the bioavailability of anthraquinones in vivo and the metabolism in liver microsomes. Materials and Methods: In vivo, we used a reliable UPLC-ESI-QqQ-MS/MS method to measure seven AQ compounds in the jugular vein plasma of rats following oral administration of HeShouWu. Furthermore, in order to quantify the bioavailability of AQs in vivo and to further understand the metabolism of these compounds, we compared the in vitro metabolism of AQ in different species with respect to metabolic profiles, the enzymes involved, and catalytic efficiency using liver microsomes from human (HLM), mouse (MLM), rat (RLM), and beagle dog (DLM). Results: We identified two metabolic pathways, including the hydroxylation and glucuronidation of AQ, in the liver microsomes of humans and other species using UPLC-ESI-Q-TOF. We found that substitutions on the AQ ring were crucial to the activity and regioselectivity of its hydroxylation. In general, hydroxylation activity decreased greatly with β-COOH (rhein) and enhanced dramatically with β-OH (emodin). We also found that glucuronidation of the compound emodin-8-O-β-D-glucoside acts as the main isoform in AQ hydroxylation in HLM and DLM. Total microsomal intrinsic clearance values for AQ were greatest in mouse microsomes, followed by those in dog, human, and rat microsomes. Conclusion: The absorption of different anthrquinone compounds varied based on the compound structure, the metabolism types and products of anthraquinones in liver microsomes were different in different species. These findings provide vital information for a deeper unuunderstanding of the metabolism of AQs.
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- 2018
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10. SpCrus2 Glycine-Rich Region Contributes Largely to the Antiviral Activity of the Whole-Protein Molecule by Interacting with VP26, a WSSV Structural Protein
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Yue Wang, Chao Zhang, Wen-Hong Fang, Hong-Yu Ma, and Xin-Cang Li
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Scylla paramamosain ,crustin ,glycine-rich region (GRR) ,cysteine-rich region (CRR) ,WSSV ,binding and antiviral activities ,Biology (General) ,QH301-705.5 - Abstract
Crustins are cysteine-rich cationic antimicrobial peptides with diverse biological functions including antimicrobial and proteinase inhibitory activities in crustaceans. Although a few crustins reportedly respond to white spot syndrome virus (WSSV) infection, the detailed antiviral mechanisms of crustins remain largely unknown. Our previous research has shown that SpCrus2, from mud crab Scylla paramamosain, is a type II crustin containing a glycine-rich region (GRR) and a cysteine-rich region (CRR). In the present study, we found that SpCrus2 was upregulated in gills after WSSV challenge. Knockdown of SpCrus2 by injecting double-stranded RNA (dsSpCrus2) resulted in remarkably increased virus copies in mud crabs after infection with WSSV. These results suggested that SpCrus2 played a critical role in the antiviral immunity of mud crab. A GST pull-down assay showed that recombinant SpCrus2 interacted specifically with WSSV structural protein VP26, and this result was further confirmed by a co-immunoprecipitation assay with Drosophila S2 cells. As the signature sequence of type II crustin, SpCrus2 GRR is a glycine-rich cationic polypeptide with amphipathic properties. Our study demonstrated that the GRR and CRR of SpCrus2 exhibited binding activities to VP26, with the former displaying more potent binding ability than the latter. Interestingly, pre-incubating WSSV particles with recombinant SpCrus2 (rSpCrus2), rGRR, or rCRR inhibited virus proliferation in vivo; moreover, rSpCrus2 and rGRR possessed similar antiviral abilities, which were much stronger than those of rCRR. These findings indicated that SpCrus2 GRR contributed largely to the antiviral ability of SpCrus2, and that the stronger antiviral ability of GRR might result from its stronger binding activity to the viral structural protein. Overall, this study provided new insights into the antiviral mechanism of SpCrus2 and the development of new antiviral drugs.
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- 2021
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11. Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis
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Ze-Dong Wang, Huan-Huan Liu, Zhan-Xi Ma, Hong-Yu Ma, Zhong-Yu Li, Zhi-Bin Yang, Xing-Quan Zhu, Bin Xu, Feng Wei, and Quan Liu
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Toxoplasma gondii ,immunocompromised patients ,HIV/AIDS patients ,cancer patients ,transplant recipients ,prevalence ,Microbiology ,QR1-502 - Abstract
Toxoplasma gondii has been suggested as an important opportunistic pathogen in immunocompromised patients. We conducted a global meta-analysis to assess the prevalence and odds ratios (ORs) of T. gondii infection in immunocompromised individuals. Electronic databases were reviewed for T. gondii infection in HIV/AIDS patients, cancer patients, and transplant recipients, and meta-analyses were conducted to calculate overall estimated prevalence and ORs using random or fixed-effects models. Totally, 72 eligible studies were included. The estimated pooled prevalence of T. gondii infection in immunocompromised patients and the control was 35.9 and 24.7% (p < 0.001), with an OR of 2.24, i.e., 42.1 and 32.0% for HIV/AIDS patients and the control (p < 0.05), 26.0 and 12.1% for cancer patients and the control (p < 0.001), and 42.1 and 34.5% for transplant recipients and the control (p > 0.05), whose estimated pooled ORs were 1.92 (95% CI, 1.44–2.55), 2.89 (95% CI, 2.36–3.55), and 1.51 (95% CI, 1.16–1.95), respectively. This study is the first to demonstrate that the immunocompromised patients are associated with higher odds of T. gondii infection, and appropriate prevention and control measures are highly recommended for these susceptible populations.
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- 2017
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12. A Method of Hepatocyte Extraction Conjugated with HPLC is Established for Screening Potential Active Components in Chinese Medicines—Probing Herba Artemisiae Scopariae as an Exemplifying Approach
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Hong-Wei Fan, Hong-Yu Ma, Xiang-Rui Wu, Yong-Qing Hua, Quan Zhu, and Min Hong
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hepatocyte extraction ,HPLC ,Herba Artemisiae Scopariae ,bifendate ,liver protection ,Organic chemistry ,QD241-441 - Abstract
In order to establish an effective and quick method for screening potential bioactive compounds in Traditional Chinese Medicines (TCMs), hepatocytes were employed for extracting either bifendate, a clinical medicine for liver diseases, or chemicals in Herba Artemisiae Scopariae (A. Scopariae), a commonly used traditional Chinese medicine for remedying liver diseases such as hepatitis induced by viruses, chemicals or alcohol. After hepatocyte extraction the compounds were analyzed by HPLC, therefore this method was referrred to as hepatocyte extraction conjugated with HPLC (HE-HPLC). In the first part of this study, HE-HPLC showed that bifendate was extracted by hepatocytes and detected by HPLC-DAD which indicated the feasibility of this method. Then in the second part of the study, the potential active components in the A. scopariae extract were studied using HE-HPLC. Six chemicals in the A. scopariae extract, which could bind to hepatocytes in vitro, were detected by HPLC-DAD and three were identified as 7-hydroxy-coumarin (7-OH-C), capillartemisin A and 7-methoxy-coumarin, respectively. In vitro assays showed that 7-OH-C protected HL-7702 hepatocytes from H2O2 injury. The results indicated that these compounds could be extracted by hepatocytes, could be detected by HPLC and more importantly were bioactive. It is suggested that HE-HPLC is a useful method for screening potent active components in Chinese medicines used to treat liver diseases.
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- 2012
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13. Two New Constituents from Artemisia capillaris Thunb.
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Yue-Hu Pei, Min Hong, Yu-Zhi Zhou, Yi Sun, and Hong-Yu Ma
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Artemisia capillaris ,p-hydroxyacetophenone ,adenine ,Organic chemistry ,QD241-441 - Abstract
Two new compounds, 6′-O-caffeoyl-p-hydroxyacetophenone-4-O-β-D-glucopyranoside(1) and 6-amino-9-[1-(3,4-dihydroxyphenyl)ethyl]-9H-purine (2) wereisolated from the aerial parts of Artemisia capillaris Thunb. The structures wereestablished on the basis of spectral data.
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- 2008
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14. N-acetylglucosamine 2-Epimerase from Pedobacter heparinus: First Experimental Evidence of a Deprotonation/Reprotonation Mechanism
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Su-Yan Wang, Pedro Laborda, Ai-Min Lu, Xu-Chu Duan, Hong-Yu Ma, Li Liu, and Josef Voglmeir
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sialic acid metabolism ,N-acetylglucosamine 2-epimerase ,deprotonation/reprotonation mechanism ,Neu5Ac analogues ,synthesis of sialic acid analogues ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
The control of cellular N-acetylmannosamine (ManNAc) levels has been postulated to be an effective way to modulate the decoration of cell surfaces with sialic acid. N-acetylglucosamine 2-epimerase catalyzes the interconversion of N-acetylglucosamine (GlcNAc) and ManNAc. Herein, we describe the cloning, expression, purification and biochemical characterization of an unstudied N-acetylglucosamine 2-epimerase from Pedobacter heparinus (PhGn2E). To further characterize the enzyme, several N-acylated glucosamine derivatives were chemically synthesized, and subsequently used to test the substrate specificity of PhGn2E. Furthermore, NMR studies of deuterium/hydrogen exchange at the anomeric hydroxy group and C-2 positions of the substrate in the reaction mixture confirmed for the first time the postulated epimerization reaction via ring-opening/enolate formation. Site-directed mutagenesis of key residues in the active site showed that Arg63 and Glu314 are directly involved in proton abstraction and re-incorporation onto the substrate. As all mechanistically relevant active site residues also occur in all mammalian isoforms, PhGn2E can serve as a model N-acetylglucosamine 2-epimerase for further elucidation of the active site mechanism in these enzymes.
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- 2016
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15. Real-Time Tool-Path Planning Using Deep Learning for Subtractive Manufacturing.
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Yi-Fei Feng 0001, Hong-Yu Ma, Li-Yong Shen, Chun-Ming Yuan, and Xin Jiang 0008
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- 2024
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16. Identification, characterization, and functional analysis of Tube and Pelle homologs in the mud crab Scylla paramamosain.
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Xin-Cang Li, Xiao-Wen Zhang, Jun-Fang Zhou, Hong-Yu Ma, Zhi-Dong Liu, Lei Zhu, Xiao-Juan Yao, Lin-Gui Li, and Wen-Hong Fang
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Medicine ,Science - Abstract
Tube and Pelle are essential components in Drosophila Toll signaling pathway. In this study, we characterized a pair of crustacean homologs of Tube and Pelle in Scylla paramamosain, namely, SpTube and SpPelle, and analyzed their immune functions. The full-length cDNA of SpTube had 2052 bp with a 1578 bp open reading frame (ORF) encoding a protein with 525 aa. A death domain (DD) and a kinase domain were predicted in the deduced protein. The full-length cDNA of SpPelle had 3825 bp with a 3420 bp ORF encoding a protein with 1140 aa. The protein contained a DD and a kinase domain. Two conserved repeat motifs previously called Tube repeat motifs present only in insect Tube or Tube-like sequences were found between these two domains. Alignments and structure predictions demonstrated that SpTubeDD and SpPelleDD significantly differed in sequence and 3D structure. Similar to TubeDD, SpTubeDD contained three common conserved residues (R, K, and R) on one surface that may mediate SpMyD88 binding and two common residues (A and A) on the other surface that may contribute to Pelle binding. By contrast, SpPelleDD lacked similar conservative residues. SpTube, insect Tube-like kinases, and human IRAK4 were found to be RD kinases with an RD dipeptide in the kinase domain. SpPelle, Pelle, insect Pelle-like kinases, and human IRAK1 were found to be non-RD kinases lacking an RD dipeptide. Both SpTube and SpPelle were highly expressed in hemocytes, gills, and hepatopancreas. Upon challenge, SpTube and SpPele were significantly increased in hemocytes by Gram-negative or Gram-positive bacteria, whereas only SpPelle was elevated by White Spot Syndrome Virus. The pull-down assay showed that SpTube can bind to both SpMyD88 and SpPelle. These results suggest that SpTube, SpPelle, and SpMyD88 may form a trimeric complex involved in the immunity of mud crabs against both Gram-negative and Gram-positive bacteria.
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- 2013
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17. A Lightweight Model for Feature Points Recognition of Tool Path Based on Deep Learning.
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Shuo-Peng Chen, Hong-Yu Ma, Li-Yong Shen, and Chun-Ming Yuan
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- 2023
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18. Optimal feedrate planning on a five-axis parametric tool path with global geometric and kinematic constraints.
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Hong-Yu Ma, Chun-Ming Yuan, Li-Yong Shen, and Xiao-Shan Gao
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- 2022
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19. A survey of path planning and feedrate interpolation in computer numerical control.
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Hong-Yu Ma, Li-Yong Shen, Xin Jiang 0008, Qiang Zou, and Chun-Ming Yuan
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- 2023
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20. Effect of Remimazolam on Emergence Delirium in Children Undergoing Laparoscopic Surgery: A Double-blinded Randomized Trial.
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Yu-Hang Cai, John Wei Zhong, Hong-Yu Ma, Szmuk, Peter, Cheng-Yu Wang, Zhen Wang, Xu-Lin Zhang, Le-Qi Dong, and Hua-Cheng Liu
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- 2024
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21. A Theoretically Complete Surface Segmentation Method for CNC Subtractive Fabrication
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Hong-Yu Ma, Chun-Ming Yuan, Li-Yong Shen, and Yi-Fei Feng
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General Medicine - Published
- 2023
22. Time-dependent quantum wave packet calculation for reaction S−(2P)+H2(1Σg+)→SH−(1Σ)+H(2S) on ab Initio potential energy surface
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Hong-yu Ma, Cheng-yuan Zhang, Yu-zhi Song, Feng-cai Ma, and Yong-qing Li
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Physical and Theoretical Chemistry - Abstract
The time-dependent wave packet propagation method was applied to investigate the dynamic behaviours of the reaction [Formula: see text] based on the electronic ground state (2A ′) potential energy surface of the SH2− ionic molecule. The collision energy dependent reaction probabilities and integral cross sections are obtained. The numerical results suggest that there are significant oscillation structures over all the studied range of the collision energies. The vibrational excitation and rotational excitation of the diatomic reagent H2 promote the reactivity significantly as suggested by the numerical total reaction probabilities with the initial rotational quantum number of j = 0, 2, 4, 6, 8, 10, and the vibrational quantum number v = 0, 1, 2, 3, 4. The numerical integral cross sections are quite consistent with the experimental data reported in previous work.
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- 2022
23. Trend of research on the medical use of molecular hydrogen: a bibliometric analysis.
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He Li, Hong-Yu Ma, Wei-Long Hua, Yong-Xin Zhang, Lei Zhang, Peng-Fei Xing, Peng-Fei Yang, and Jian-Min Liu
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BIBLIOMETRICS , *HYDROGEN analysis , *MEDICAL research , *COVID-19 , *GUT microbiome - Abstract
The medical use of molecular hydrogen, including hydrogen-rich water and hydrogen gas, has been extensively explored since 2007. This article aimed to demonstrate the trend in medical research on molecular hydrogen. A total of 1126 publications on hydrogen therapy were retrieved from the PubMed database until July 30, 2021. From 2007 to 2020, the number of publications in this field had been on an upward trend. Medical Gas Research, Scientific Report and Shock have contributed the largest number of publications on this topic. Researchers by the name of Xue-Jun Sun, Ke-Liang Xie and Yong-Hao Yu published the most studies in the field. Analysis of the co-occurrence of key words indicated that the key words “molecular hydrogen,” “hydrogen-rich water,” “oxidative stress,” “hydrogen gas,” and “inflammation” occurred most frequently in these articles. “Gut microbiota,” “pyroptosis,” and “COVID-19” occurred the most recently among the keywords. In summary, the therapeutic application of molecular hydrogen had attracted much attention in these years. The advance in this field could be caught up by subscribing to relevant journals or following experienced scholars. Oxidative stress and inflammation were the most important research directions currently, and gut microbiota, pyroptosis, and coronavirus disease 2019 might become hotspots in the future. [ABSTRACT FROM AUTHOR]
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- 2023
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24. Novel predictors and a predictive model of cerebrovascular atherosclerotic ischemic stroke based on clinical databases
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He Li, Pei Liu, Hong-Yu Ma, Wei-Long Hua, Yong-Xin Zhang, Lei Zhang, Yong-Wei Zhang, Bo Hong, Peng-Fei Yang, and Jian-Min Liu
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Neurology ,Neurology (clinical) ,General Medicine - Abstract
Early identification of cerebrovascular atherosclerotic ischemic stroke is necessary for accurate treatment and clinical research.To identify novel predictors and build a predictive model of ischemic strokes due to cerebrovascular atherosclerosis.MIMIC-IV database was used to search for clinical data of patients with ischemic stroke. Included patients were divided into two groups according to their etiologies. Univariate and multivariate logistic regressions were used to build the predictive model, and the model reliability parameters were calculated. The cut-off value for the model was selected according to the Youden index. Clinical data from the Neurovascular Center of Changhai Hospital were used to verify the predictive model.Logistical regressions showed a positive correlation between advanced age, peripheral atherosclerosis, history of transient ischemia, and the diagnosis of ischemic strokes due to cerebrovascular atherosclerosis. The history of atrial fibrillation, levels of the National Institutes of Health Stroke Scale, serum potassium, and activated partial thromboplastin time were negatively correlated to the diagnosis of cerebrovascular atherosclerotic ischemic stroke. The predictive model was constructed from logistic regression results, and the area under the curve was 0.764. The cut-off value for the model was set at 0.089 to achieve the highest Youden index, with sensitivity and specificity of 75.9% and 64.1%. Clinical verification of the model revealed that the sensitivity and specificity of the model were 52.5% and 93.0% respectively.The efficacy of the predictive model was acceptable as an aid in predicting cerebrovascular atherosclerotic ischemic stroke.
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- 2022
25. Tool Path Planning with Confined Scallop Height Error Using Optimal Connected Fermat Spirals
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Hong-Yu Ma, Chun-Ming Yuan, and Li-Yong Shen
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Statistics and Probability ,Computational Mathematics ,Applied Mathematics - Published
- 2022
26. Rosuvastatin Enhances Alveolar Fluid Clearance in Lipopolysaccharide-Induced Acute Lung Injury by Activating the Expression of Sodium Channel and Na,K-ATPase via the PI3K/AKT/Nedd4-2 Pathway
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Yang Ye, Qian Wang, Xi-Ya Ren, Hong-Xia Mei, Fang-gao Smith, Hao-Ran Xu, Qian Yang, Yan Chen, Pu-Hong Zhang, Shengwei Jin, Hong-Yu Ma, Shu-Yang Xiang, Chen-xi Shen, and Ke-Wen Xu
- Subjects
0301 basic medicine ,ARDS ,Lipopolysaccharide ,Immunology ,Lung injury ,Pharmacology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,In vivo ,medicine ,Immunology and Allergy ,Rosuvastatin ,Na+/K+-ATPase ,Protein kinase B ,PI3K/AKT/mTOR pathway ,Original Research ,alveolar fluid clearance ,nutritional and metabolic diseases ,acute respiratory distress syndrome ,respiratory system ,medicine.disease ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Na,K-ATPases ,Journal of Inflammation Research ,rosuvastatin ,sodium channel ,medicine.drug - Abstract
Hao-Ran Xu,1 Qian Yang,1 Shu-Yang Xiang,1 Pu-Hong Zhang,1 Yang Ye,1 Yan Chen,1 Ke-Wen Xu,2 Xi-Ya Ren,2 Hong-Xia Mei,1 Chen-Xi Shen,1 Hong-Yu Ma,1 Fang-Gao Smith,1,3 Sheng-Wei Jin,1 Qian Wang1 1Department of Anesthesia and Critical Care, The Second Afï¬liated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People’s Republic of China; 2Wenzhou Medical University, Wenzhou, People’s Republic of China; 3Institute of Inflammation and Aging, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UKCorrespondence: Sheng-Wei Jin; Qian WangDepartment of Anesthesia and Critical Care, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 325027, People’s Republic of ChinaTel +86 577-88002806Fax +86 577-88832693Email jsw@wmu.edu.cn; wqian84@163.comBackground: Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are devastating clinical conditions characterized by pulmonary epithelial damage and protein-rich fluid accumulation in the alveolar spaces. Statins are a class of HMG-CoA reductase inhibitors, which exert cholesterol-lowering and anti-inflammatory effects.Methods: Rosuvastatin (1 mg/kg) was injected intravenously in rats 12 h before lipopolysaccharide (LPS, 10 mg/kg) administration. Eight hours later after LPS challenge, alveolar fluid clearance (AFC) was detected in rats (n = 6– 8). Rosuvastatin (0.3 μmol/mL) and LPS were cultured with primary rat alveolar type II epithelial cells for 8 h.Results: Rosuvastatin obviously improved AFC and attenuated lung-tissue damage in ALI model. Moreover, it enhanced AFC by increasing sodium channel and Na,K-ATPase protein expression. It also up-regulated P-Akt via reducing Nedd4-2 in vivo and in vitro. Furthermore, LY294002 blocked the increase in AFC in response to rosuvastatin. Rosuvastatin-induced AFC was found to be partly rely on sodium channel and Na,K-ATPase expression via the PI3K/AKT/Nedd4-2 pathway.Conclusion: In summary, the findings of our study revealed the potential role of rosuvastatin in the management of ALI/ARDS.Keywords: acute respiratory distress syndrome, rosuvastatin, alveolar fluid clearance, sodium channel, Na,K-ATPases
- Published
- 2021
27. Comparison of four markers of hepatic fibrosis and hepatic function indices in patients with liver cirrhosis and hepatoma
- Author
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Ruo-Yi Li, Shou-Zhen Quan, Lei Dong, Hong-Yu Ma, and Xiang-Rong Wang
- Subjects
Collagen Type IV ,Liver Cirrhosis ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,Cirrhosis ,medicine.drug_class ,Bilirubin ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Fibrosis ,Internal medicine ,medicine ,Humans ,Advanced and Specialized Nursing ,Bile acid ,business.industry ,Albumin ,medicine.disease ,Anesthesiology and Pain Medicine ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,Alkaline phosphatase ,030211 gastroenterology & hepatology ,Laminin ,business ,Hepatic fibrosis ,Biomarkers ,TBIL - Abstract
Background The present study aimed to compare four hepatic fibrosis markers [i.e., hyaluronic acid (HA), laminin (LN), procollagen III N-terminal peptide (PIIINP), and collagen type IV (CIV)] and 16 hepatic function indices in patients with liver cirrhosis of varying etiology. Methods The hepatic function indices and hepatic fibrosis markers were measured in 108 patients with liver cirrhosis and hepatoma using an automatic biochemical analyzer and luminescent immune analyzer. Twenty healthy controls were enrolled to compare the differences between liver cirrhosis and hepatoma of varying etiology and to analyze the correlations between the hepatic function indices and fibrosis markers. Results There was no correlation between alanine aminotransferase (ALT), total protein (TP), alkaline phosphatase (ALP), or the four markers of hepatic fibrosis in liver cirrhosis caused by hepatitis B (P>0.05). Aspartate aminotransferase (AST) was positively correlated with HA (r=0.428, P=0.007), LN (r=0.458, P=0.004), and CIV (r=0.374, P=0.021). Total bilirubin (TBIL) and direct bilirubin (DBIL) were positively correlated with LN (TBIL: r=0.480, P=0.002; DBIL: r=0.457, P=0.004), PIIINP (TBIL: r=0.380, P=0.017; DBIL: r=0.406, P=0.011), and CIV (TBIL: r=0.415, P=0.010; DBIL: r=0.400, P=0.013). Total bile acid (TBA) and γ-glutamyltranspeptidase (GGT) were positively correlated with PIIINP (TBA: r=0.363, P=0.025; GGT: r=0.353, P=0.029) and CIV (TBA: r=0.419, P=0.009; GGT: r=0.335, P=0.040). Leucine aminopeptidase (LAP) was positively correlated with LN (r=0.482, P=0.002). Cholinesterase (CHE) (HA: r=-0.452, P=0.004, LN: r=-0.336, P=0.039; PIIINP: r=-0.468, P=0.003; CIV: r=-0.485, P=0.002), prealbumin (PA) (HA: r=-0.575, P=0.000, LN: r=-0.413, P=0.010; PIIINP: r=-0.344, P=0.035; CIV: r=-0.371, P=0.022), albumin (ALB) (HA: r=-0.541, P=0.000, LN: r=-0.373, P=0.021; PIIINP: r=-0.353, P=0.030; CIV: r=-0.415, P=0.010), and superoxide dismutase (SOD) (HA: r=-0.334, P=0.040, LN: r=-0.347, P=0.033; PIIINP: r=-0.487, P=0.002; CIV: r=-0.536, P=0.001) were negatively correlated with the four markers of hepatic fibrosis. There was no correlation between ALT, AST, TBIL, TP, ALP, GGT, or the four hepatic fibrosis markers in hepatoma caused by hepatitis B (P>0.05). Meanwhile, DBIL and TBA were positively correlated with CIV (DBIL: r=0.519, P=0.023; TBA: r=0.563, P=0.012), while CHE (r=-0.604, P=0.006), ALB (r=-0.564, P=0.012), and SOD (r=-0.489, P=0.034) were negatively correlated with CIV. Moreover, PA was negatively correlated with LN (r=-0.510, P=0.026) and CIV (r=-0.696, P=0.001). Conclusions The concentrations of the serological indices differed significantly based on the specific liver cirrhosis etiology. There was a strong correlation between the hepatic function indices and four hepatic fibrosis markers. Thus, the detection of these markers might improve the diagnosis and treatment of hepatoma.
- Published
- 2021
28. Effects of Dynamic Plasma Sheath on Electromagnetic Wave Propagation and Bit Error Rate Under External Magnetic Field
- Author
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Ying-Min Yi, Zi-Han Jiao, Jiang-Fan Liu, Guang-Hui Bai, Hong-Yu Ma, Xiaoli Xi, and Yun Fang
- Subjects
Physics ,Nuclear and High Energy Physics ,Debye sheath ,Electron density ,Frequency-shift keying ,Wave propagation ,Keying ,Plasma ,Condensed Matter Physics ,01 natural sciences ,010305 fluids & plasmas ,Computational physics ,symbols.namesake ,0103 physical sciences ,symbols ,Transmission coefficient ,Phase-shift keying - Abstract
The “magnetic window” is considered to be an effective technique for alleviating a vehicle’s atmospheric reentry blackout, whereas the dynamic variation characteristic of the plasma sheath is critical for the vehicle’s communication. In this article, the effects of dynamic plasma sheath on electromagnetic wave propagation and the bit error rate (BER) of two typical modulation signals under an external magnetic field are investigated. First, the hybrid matrix method is used to calculate the transmission coefficients of right-handed circularly polarized (RHCP) waves with different magnetic fields and plasma electron density fluctuation. Then, the average BER of two typical modulations signals, namely M-ary phase-shift keying (MPSK) and noncoherent M-ary frequency shift keying modulation (NC-MFSK) is numerically simulated based on the magnetized plasma sheath channel response. The results show that the magnitude in dB scale and phase of the RHCP transmission coefficient follow Gaussian distribution when the probability density function of the electron density is a Gaussian distribution. The BER performance of binary phase-shift keying (BPSK) and NC-MFSK is relevant to the incident wave frequency, the plasma stopband, and the intensity of electron density variation. Owing to the insensitivity of phase jitter, the NC-8FSK has a better performance than BPSK, followed by NC-4FSK and NC-2FSK.
- Published
- 2020
29. Study on Logging Identification Method of Complex Lithology in X Oilfield
- Author
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Ji-wei Ding, Hong-yu Ma, Qing-shan Yang, Yan Lu, and Shu-jun Yin
- Published
- 2022
30. Trend of research on the medical use of molecular hydrogen: a bibliometric analysis
- Author
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Jian-Min Liu, Peng-Fei Yang, He Li, Hong-Yu Ma, Wei-Long Hua, Yong-Xin Zhang, Lei Zhang, and Peng-Fei Xing
- Subjects
Anesthesiology and Pain Medicine ,Neuroscience (miscellaneous) - Published
- 2022
31. Differentially Expressed Protein Are Involved in Dynamic Changes of Catechins Contents in Postharvest Tea Leaves under Different Temperatures
- Author
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Hong-Yu Ma, Yong-Xin Wang, Rui-Min Teng, Jing Zhuang, and Zhi-Jun Wu
- Subjects
Proteomics ,0106 biological sciences ,Chemistry ,010401 analytical chemistry ,Temperature ,General Chemistry ,respiratory system ,complex mixtures ,01 natural sciences ,Camellia sinensis ,Catechin ,0104 chemical sciences ,Plant Leaves ,Temperature treatment ,Gene Expression Regulation, Plant ,Gene expression ,Postharvest ,Food science ,KEGG ,Transcriptome ,General Agricultural and Biological Sciences ,Plant Proteins ,010606 plant biology & botany - Abstract
In this study, isobaric tags for relative and absolute quantitation (iTRAQ) technology were used to investigate three samples from postharvest tea leaves that were treated at room temperature (25 °C, control group), high temperature (38 °C), and low temperature (4 °C) for 4 h. In heat and cold treatments, a total of 635 and 566 differentially expressed proteins (DEPs) were determined, respectively. DEPs were annotated to GO and KEGG databases, which revealed that DEPs involved in various aspects of biological process. Three catechins-related DEPs, CsCHI, CsF3H, and CsANR, were identified. Both catechins contents and the expression profiles of catechins biosynthesis-related genes changed significantly under different temperature treatments. The correlations between catechins contents, gene expression profiles, and DEPs were analyzed. This study provides potential new insights into the molecular basis for tea production of postharvest leaves and catechins content changes at diverse temperature conditions and will guide the improvement of tea-processing technology.
- Published
- 2019
32. LXA4 Inhibits Lipopolysaccharide-Induced Inflammatory Cell Accumulation by Resident Macrophages in Mice
- Author
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Qian Yang, Qian Wang, Hao-Ran Xu, Shu-Yang Xiang, Yang Ye, Shengwei Jin, Hong-Yu Ma, and Hong-Xia Mei
- Subjects
0301 basic medicine ,ARDS ,Lipopolysaccharide ,Immunology ,CCL2 ,Pharmacology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Immunology and Allergy ,Macrophage ,Receptor ,Original Research ,lipoxin A4 ,lipopolysaccharide ,neutrophil ,medicine.disease ,CXCL2 ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,Alveolar macrophage ,Tumor necrosis factor alpha ,resident macrophage ,recruited macrophage ,Journal of Inflammation Research - Abstract
Hong-Xia Mei, Yang Ye, Hao-Ran Xu, Shu-Yang Xiang, Qian Yang, Hong-Yu Ma, Sheng-Wei Jin, Qian Wang Department of Anesthesia and Critical Care, The Second Afï¬liated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, People’s Republic of ChinaCorrespondence: Sheng-Wei Jin; Qian WangDepartment of Anesthesia and Critical Care, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, 109 Xueyuan Road, Wenzhou, Zhejiang Province, 325027, People’s Republic of ChinaTel +86-577-88002806Fax +86-577-88832693Email jinshengwei69@163.com; wqian84@163.comIntroduction: Alveolar macrophages that regulate the inflammatory response in lungs are the main target cell for the treatment of inflammatory pulmonary pathologies, such as acute respiratory distress syndrome (ARDS). Yolk sac derived alveolar resident macrophages play an important role in the pulmonary inflammatory response. With regards to anti-inflammatory actions, lipoxin A4 (LXA4) has been identified as an inflammatory “braking signal”.Methods: In vivo, LXA4 (0.1 μg/mouse) was injected intraperitoneally after intratracheal (1 mg/kg) lipopolysaccharide (LPS) administration; flow cytometry was used to measure peripheral blood monocyte derived recruited macrophage and neutrophil numbers; resident alveolar macrophage was depleted by liposome clodronate; CXCL2, CCL2, MMP9 level was detected by RT-PCR and ELISA. In vitro, sorted resident macrophages (1× 106) were cultured with LPS (1 μg/mL) and LXA4 (100 nmol/mL) with or without BOC-2 (10 μM) for 24 h to gain a better understanding of the mechanisms of LXA4.Results: LXA4 inhibited tumor necrosis factor-a (TNF-a) and interleukin-1β (IL-1β) production induced by LPS. LXA4 also mediated LPS-induced macrophage recruitment and showed that this was dependent on CCL2 secretion and release by resident macrophages. LXA4 protects lung tissue by inhibiting neutrophil recruitment, partly through the CXCL2/MMP-9 signaling pathway. CXCL2 and MMP-9 are mainly expressed by resident macrophages and neutrophils, respectively. Finally, LXA4’s beneficial effects were abrogated by BOC-2, an LXA4 receptor inhibitor.Conclusion: These results suggest that LXA4 may be a promising therapy for preventing and treating ARDS.Keywords: resident macrophage, recruited macrophage, neutrophil, lipoxin A4, lipopolysaccharide
- Published
- 2021
33. Interleukin-6 RNA knockdown ameliorates acute lung injury induced by intestinal ischemia reperfusion in rats by upregulating interleukin-10 expression
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Liu‑Lin Xiong, Ting‑Hua Wang, Yun‑Hui Zhang, Mu‑Dong Wen, Bing Yuan, Piao Zhang, and Hong‑Yu Ma
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,intestinal ischemia/reperfusion ,interleukin-10 ,Acute Lung Injury ,Ischemia ,Biology ,Lung injury ,Pharmacology ,Biochemistry ,Proinflammatory cytokine ,Small hairpin RNA ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,RNA interference ,Genetics ,medicine ,Animals ,Interleukin 6 ,Molecular Biology ,Lung ,Gene knockdown ,Interleukin-6 ,Articles ,medicine.disease ,Up-Regulation ,Intestines ,Interleukin 10 ,030104 developmental biology ,RNAi Therapeutics ,Oncology ,030220 oncology & carcinogenesis ,Reperfusion Injury ,biology.protein ,Molecular Medicine - Abstract
Acute lung injury (ALI) is a common complication following intestinal ischemia/reperfusion (II/R) injury and contributes to the associated high mortality rate. However, the underlying mechanism is poorly understood and treatments are limited. RNA interference (RNAi) has been demonstrated to provide a promising disease treatment strategy both in vitro and in vivo. Therefore, the present study aimed to test whether blocking the proinflammatory cytokine IL‑6 by RNAi may protect the lungs from remote organ injury following II/R, and to investigate the potential underlying mechanisms. A total of 176 adult healthy male Sprague‑Dawley rats were randomly divided into sham, II/R, negative‑control and IL‑6‑short hairpin (sh)RNA groups. The rats underwent II/R injury with occlusion of the superior mesenteric artery and coeliac artery to induce ischemia for 40 min, and were subsequently reperfused for 0‑48 h. The negative‑control group received a control lentiviral vector containing scrambled or non‑specific sequences, and the IL‑6‑shRNA groups were administered with a vector containing an IL‑6 shRNA sequence to affect RNAi‑mediated knockdown of IL‑6. ALI severity was determined by lung edema (lung wet/dry ratio) and histological analysis (lung injury scores). IL‑6 localization, and mRNA and protein expression levels, were detected by immunofluorescence, reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. IL‑10 expression induced by IL‑6 knockdown in lung tissues was additionally detected. IL‑6 RNAi was revealed to significantly reduce the expression of IL‑6, which was associated with upregulated IL‑10 expression in lung tissues. Consequently, the severities of ALI and edema induced by II/R were substantially improved. In conclusion, the present study demonstrated that IL‑6 RNAi may protect the lung from ALI induced by II/R, and that this protective role may be associated with upregulation of IL‑10. These findings may contribute to the development of an IL‑6‑RNAi‑based therapeutic strategy for the treatment of II/R‑induced ALI.
- Published
- 2017
34. MicroRNAs Involved in the Regulation of LC-PUFA Biosynthesis in Teleosts: miR-33 Enhances LC-PUFA Biosynthesis in Siganus canaliculatus by Targeting insig1 which in Turn Upregulates srebp1
- Author
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Jun Jun Sun, Li Guo Zheng, Qing Hao Zhang, Shuqi Wang, Jin Ying Zhang, Óscar Monroig, Douglas R. Tocher, Hong Yu Ma, Cui Ying Chen, Yuan You Li, Cui Hong You, National Key Research and Development Program (China), National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province, Agriculture Research System of China, Guandong Academy Of Agricultural Sciences, Monroig, Óscar [0000-0001-8712-0440], Tocher, Douglas R. [0000-0002-8603-9410], Monroig, Óscar, and Tocher, Douglas R.
- Subjects
0106 biological sciences ,0301 basic medicine ,miR-33 ,Fatty Acid Desaturases ,Fish Proteins ,FADS2 ,Genetic Vectors ,Gene Expression ,Srebp1 ,Biology ,Δ6Δ5fads2 ,srebp1 ,01 natural sciences ,Applied Microbiology and Biotechnology ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Downregulation and upregulation ,Biosynthesis ,010608 biotechnology ,microRNA ,Animals ,Humans ,Cloning, Molecular ,Promoter Regions, Genetic ,3' Untranslated Regions ,Gene knockdown ,Insig1 ,insig1 ,biology.organism_classification ,Lipid Metabolism ,LC-PUFA biosynthesis ,Recombinant Proteins ,Sterol regulatory element-binding protein ,Cell biology ,Perciformes ,MicroRNAs ,030104 developmental biology ,HEK293 Cells ,chemistry ,Gene Expression Regulation ,elovl5 ,Fatty Acids, Unsaturated ,Hepatocytes ,Elovl5 ,Sterol Regulatory Element Binding Protein 1 ,Rabbitfish ,Signal Transduction - Abstract
Post-transcriptional regulatory mechanisms play important roles in the regulation of LC-PUFA biosynthesis. Our previous study revealed that miR-33 could increase the expression of fatty acyl desaturases (fads2) in the rabbitfish Siganus canaliculatus, but the specific mechanism is unknown. Here, we confirmed that miR-33 could target the 3′UTR of insulin-induced gene 1 (insig1), resulting in downregulation of its protein level in the rabbitfish hepatocyte line (SCHL). In vitro overexpression of miR-33 inhibited the mRNA level of insig1 and increased the mRNA levels of Δ6Δ5 fads2 and elovl5, as well as srebp1. In SCHL cells, proteolytic activation of sterol-regulatory-element-binding protein-1 (Srebp1) was blocked by Insig1, with overexpression of insig1 decreasing mature Srebp1 level, while inhibition of insig1 led to the opposite effect. Srebp1 could enhance the promoter activity of Δ6Δ5 fads2 and elovl5, whose expression levels decreased with knockdown of srebp1 in SCHL. Overexpression of miR-33 also resulted in a higher conversion of 18:3n-3 to 18:4n-3 and 20:5n-3 to 22:5n-3, linked to desaturation and elongation via Δ6Δ5 Fads2 and Elovl5, respectively. The results suggested that the mechanism by which miR-33 regulates LC-PUFA biosynthesis in rabbitfish is through enhancing the expression of srebp1 by targeting insig1. The findings here provide more insight to the mechanism of miRNAs involvement in the regulation of LC-PUFA biosynthesis in teleosts., This work was financially supported by the National Key R&D Program of China (2018YFD0900400), National Natural Science Foundation of China (No. 31873040 and No. 31702357), Natural Science Foundation of Guangdong Province (2018A030313910), China Agriculture Research System (CARS-47) and Guangdong Agriculture Research System (Freshwater Fish).
- Published
- 2019
35. Identification and Characterization of Two Novel Alpha-D-Galactosidases from Pedobacter heparinus
- Author
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Hong-Yu Ma, Josef Voglmeir, Li Liu, Yong-Mei Lv, Anna Kulinich, and Si Liu
- Subjects
chemistry.chemical_classification ,food.ingredient ,Galactosidases ,Pedobacter heparinus ,General Medicine ,medicine.disease_cause ,Biochemistry ,chemistry.chemical_compound ,food ,Enzyme ,chemistry ,Structural Biology ,Galactose ,Hydrolase ,medicine ,Glycosyl ,Sodium dodecyl sulfate ,Pedobacter - Abstract
Two putative α-D-galactosidases (α-GALs) belonging to glycosyl hydrolase family 27, and originating from the rather unexplored bacterial strain Pedobacter heparinus, were cloned and biochemically characterized. The recombinant enzymes designated as PhAGal729 and PhAGal2920 showed comparable biochemical properties: the optimum pH values were determined to be pH 5.0 and 5.5, and temperature optima lay between 30°C and 37°C, respectively. Both α-GALs were not dependent on the presence of divalent metal ions, and the addition of EDTA had no influence on enzymatic activity. The activity of both enzymes substantially increased in the presence of Fe3(+) ions. Both enzymes were inhibited by sodium dodecyl sulfate (SDS) and urea. α-GALs from P. heparinus were highly specific in hydrolyzing glycosides with α-1,2/3/4 or α-1,6-linked galactose to other sugars, whereas other glycosides such as α-linked N-acetylgalactosamine, N-acetylglucosamine or glucose residues were not released. Nevertheless, neither PhAGal729 nor PhAGal2920 were able to remove α-linked galactose epitopes from native human erythrocytes. The facile expression and purification procedures in combination with wide substrate specificities make α-GALs from P. heparinus potential candidates for applications in analytical research, and food- and biotechnology.
- Published
- 2015
36. Transcriptome sequencing analysis of SH-SY5Y cells infected with EV71 reveals the potential neuropathic mechanisms
- Author
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Jingyuan Fan, Zhenming Huang, Zheng Deng, Hong-Yu Ma, Yunhui Zhang, Jie Song, Yanyan Xu, Ruian Yang, and Yajie Hu
- Subjects
Nervous system ,Cancer Research ,SH-SY5Y ,Disease ,Biology ,Nervous System ,Pathogenesis ,Transcriptome ,Neuroblastoma ,03 medical and health sciences ,Cell Line, Tumor ,Virology ,medicine ,Humans ,Gene Regulatory Networks ,Gene ,030304 developmental biology ,Genetics ,0303 health sciences ,030306 microbiology ,Mechanism (biology) ,Gene Expression Profiling ,Enterovirus A, Human ,Metabolic pathway ,Infectious Diseases ,medicine.anatomical_structure ,Host-Pathogen Interactions ,Hand, Foot and Mouth Disease - Abstract
Enterovirus A71 (EV71) remains the most common causative agent of hand, foot, and mouth disease (HFMD), and the neurological complications induced by EV71 are usually the leading cause of death in children with HFMD. However, the mechanism of nervous system changes caused by EV71 infection is still unclear. Therefore, in the current study, EV71 was inoculated into the human neuroblastoma cell line SH-SY5Y and subsequent transcriptome sequencing was used to examine the alterations of the transcriptome in infected SH-SY5Y cells. It is expected to determine the underlying mechanism of neurological diseases in response to EV71 infection. As a result, a total of 82,406,974, 112,410,808 and 87,780,371 clean reads were found in the control, EV71-12 h and EV71-24 h groups, respectively. Moreover, 160 and 745 differentially expressed genes were identified in the EV71-12 h and EV71-24 h groups, respectively, as compared to the control group. Next, to further explore the pathogenic mechanism triggered by EV71 infection, we mainly focused on the common differentially expressed genes at different time points of EV71 infection. And it was discovered that there were 95 common differentially expressed genes, which were used to conduct GO and pathway analysis. GO enrichment analysis demarcated related biological processes, molecular functions and cellular components, and KEGG pathway analysis enabled annotations of metabolic pathways and revealed interactions among the significantly enriched pathways. The results showed that the enriched GO term “Nervous system development” and enriched pathway “CCKR signaling map” might be important contributors to EV71-induced neuropathological mechanisms. In addition, we also screened 10 up- and down-regulated non-protein coding genes with significantly different expression in our transcriptome profiling, which suggested that these abnormally regulated non-protein-encoding genes might also play important roles in the pathogenesis of EV71 infection. Eventually, RT-qPCR technology was adopted to validate the transcriptome sequencing data and the experiment demonstrated that the RT-qPCR and transcriptome sequencing results were basically consistent. In summary, this is the first transcriptome analysis of SH-SY5Y cells in response to EV71 infection and provides valuable cues for further exploring the mechanism of nervous system changes caused by EV71 infection.
- Published
- 2020
37. Comparative Efficacy and Safety of Anticoagulant Therapy in Patients Planned for Percutaneous Coronary Intervention: A Network Meta-Analysis of Randomized Trials
- Author
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Zhen Wang, Bin Lai, Xiao-feng Wang, Yi Li, Xiaowei Jia, Hong-Yu Ma, Rui Bai, Yujie Wei, and Huiliang Liu
- Subjects
medicine.medical_specialty ,Randomized controlled trial ,Anticoagulant therapy ,business.industry ,law ,Internal medicine ,Meta-analysis ,medicine.medical_treatment ,Medicine ,Percutaneous coronary intervention ,In patient ,business ,law.invention - Published
- 2018
38. Effects of microvirin monomers and oligomers on hepatitis C virus
- Author
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Xu-Chu Duan, Yi-Dan Zhou, Zhi-Peng Cai, Wang Meng, Li Liu, Xiao-Lian Zhang, Yuan-Qin Min, Josef Voglmeir, Anna Kulinich, and Hong-Yu Ma
- Subjects
0301 basic medicine ,hepatitis C virus ,Glycan ,Microcystis ,Hepatitis C virus ,Biophysics ,Sequence (biology) ,Peptide ,Hepacivirus ,medicine.disease_cause ,Biochemistry ,Antiviral Agents ,Mannose-Binding Lectin ,Neutralization ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Bacterial Proteins ,medicine ,Humans ,Cloning, Molecular ,Molecular Biology ,Research Articles ,chemistry.chemical_classification ,biology ,Cell Biology ,lectin oligomer ,Hepatitis C ,Recombinant Proteins ,Microvirin ,030104 developmental biology ,Monomer ,chemistry ,biology.protein ,high-mannose glycan ,Protein Multimerization ,Glycoprotein ,Linker ,Research Article - Abstract
Microvirin (MVN) is a carbohydrate-binding protein which shows high specificity for high-mannose type N-glycan structures. In the present study, we tried to identify whether MVN could bind to high-mannose containing hepatitis C virus (HCV) envelope glycoproteins, which are heavily decorated high-mannose glycans. In addition, recombinantly expressed MVN oligomers in di-, tri- and tetrameric form were evaluated for their viral inhibition. MVN oligomers bound more efficiently to HCV virions, and displayed in comparison with the MVN monomer a higher neutralization potency against HCV infection. The antiviral effect was furthermore affected by the peptide linker sequence connecting the MVN monomers. The results indicate that MVN oligomers such as trimers and tetramers may be used as future neutralization agents against HCV infections.
- Published
- 2017
39. Comparative proteomic analysis provides novel insights into chlorophyll biosynthesis in celery under temperature stress
- Author
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Guang-Long Wang, Ai-Sheng Xiong, Feng Wang, Wei Huang, Qian Jiang, Ying Huang, Yan Li, and Hong-Yu Ma
- Subjects
0106 biological sciences ,0301 basic medicine ,Chlorophyll ,Proteomics ,Physiology ,Protein metabolism ,Lyases ,macromolecular substances ,Plant Science ,Biology ,Photosynthesis ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Biosynthesis ,Gene Expression Regulation, Plant ,polycyclic compounds ,Genetics ,Intramolecular Transferases ,Apium ,Plant Proteins ,Abiotic stress ,Temperature ,food and beverages ,Apium graveolens ,Cell Biology ,General Medicine ,Plant cell ,Plant Leaves ,030104 developmental biology ,Magnesium chelatase ,chemistry ,Biochemistry ,Seedlings ,010606 plant biology & botany - Abstract
Chlorophyll (Chl) is essential for light harvesting and energy transduction in photosynthesis. A proper amount of Chl within plant cells is important to celery (Apium graveolens) yield and quality. Temperature stress is an influential abiotic stress affecting Chl biosynthesis and plant growth. There are limited proteomic studies regarding Chl accumulation under temperature stress in celery leaves. Here, the proteins from celery leaves under different temperature treatments (4, 25 and 38°C) were analyzed using a proteomic approach. There were 71 proteins identified through MALDI-TOF-TOF analysis. The relative abundance of proteins involved in carbohydrate and energy metabolism, protein metabolism, amino acid metabolism, antioxidant and polyamine biosynthesis were enhanced under cold stress. These temperature stress-responsive proteins may establish a new homeostasis to enhance temperature tolerance. Magnesium chelatase (Mg-chelatase) and glutamate-1-semialdehyde aminotransferase (GSAT), related to Chl biosynthesis, showed increased abundances under cold stress. Meanwhile, the Chl contents were decreased in heat- and cold-stressed celery leaves. The inhibition of Chl biosynthesis may be due to the downregulated mRNA levels of 15 genes involved in Chl biosynthesis. The study will expand our knowledge on Chl biosynthesis and the temperature tolerance mechanisms in celery leaves.
- Published
- 2017
40. Evaluation of immune effect of recombinant fusion protein targeting the prostate stem cell antigen based on PSCA and HSP70
- Author
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Lei DONG, Juan LIU, Hong-yu MA, Shou-zhen QUAN, Jian-xiao LV, Ying LI, and Mei-cai ZHU
- Subjects
lcsh:R5-920 ,neoplasms, experimental ,HSP70 heat-shock proteins ,prostate stem cell antigen ,lcsh:R ,lcsh:Medicine ,lcsh:Medicine (General) - Abstract
Objective To explore the immune effect and antitumor activity of recombinant prostate stem cell protein (PSCA) and heat shock protein 70 (HSP70) in a murine model of prostate cancer. Methods Twenty-five healthy male C57BL/6 mice were randomly divided into 5 groups (5 each): PSCA, HSP, PSCA+HSP, PSCA-HSP and control group. Mice in the first 4 groups were vaccinated with the corresponding proteins, and those in control group were faked with injection of phosphate buffer saline (PBS). After immunization with recombinant proteins, the PSCA-specific cellular immune responses were monitored with ELISPOT, intracellular cytokine staining assay, and flow cytometry, and ELISA assay was used to detect humoral immune responses. The tumor growth and survival of vaccined mice were observed. Results ELISPOT revealed that the mice in PSCA-HSP group generated much more IFN-γ spot-forming cells than those in other groups (P
- Published
- 2014
41. Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China
- Author
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Mingxin Song, Bo Wang, Hong-Yu Ma, Jun Qian, Huan-Huan Liu, Shuchao Wang, Zedong Wang, Feng Wei, Zheng Zeng, Zhongyu Li, and Quan Liu
- Subjects
0301 basic medicine ,Microbiology (medical) ,Veterinary medicine ,Anaplasma ,Haemaphysalis concinna ,animal diseases ,030231 tropical medicine ,030106 microbiology ,Ehrlichia ,lcsh:QR1-502 ,Babesia ,Tick ,Ixodes persulcatus ,Hepatozoon ,Microbiology ,lcsh:Microbiology ,03 medical and health sciences ,0302 clinical medicine ,parasitic diseases ,medicine ,Northeastern China ,Original Research ,Tick-borne disease ,biology ,bacterial infections and mycoses ,medicine.disease ,biology.organism_classification ,Virology ,Tick-Borne Diseases ,Haemaphysalis longicornis - Abstract
Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.
- Published
- 2016
42. Fairness preferences in the Ultimate Game: A dual-system theory perspective
- Author
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Hui, ZHANG, primary, Hong-yu, MA, primary, Fu-ming, XU, primary, Yanjun, LIU, primary, and Yan-wei, Shi, primary
- Published
- 2018
- Full Text
- View/download PDF
43. Spatial and Temporal Distribution and Air Quality Evaluation of the Indoor Air Microorganisms in Summer Campus
- Author
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Hui Min, Hong Yu Ma, Xin Ying Wang, Ji Zhao, and Ji Fei Xu
- Subjects
Indoor air ,business.industry ,Microorganism ,Living environment ,General Engineering ,Environmental engineering ,Distribution (economics) ,Inner mongolia ,Toxicology ,Fourth floor ,Environmental science ,business ,Air quality index ,Morning - Abstract
The campus indoor environment is an important learning and living environment for college students, and it is closely related to the health of college students. Using natural sedimentation to monitor and evaluate the indoor air microorganisms of four classrooms (0407, 0408, 0409 and 0410) which are in the Inner Mongolia University campus comprehensive building of fourth floor. The results show that the concentration variation of indoor air microorganisms is different in one day, in the 5 periods of time from morning to night, the maximum is 2.0×103 cfu·m-3 in the morning, which is 12 times to the minimum. Air microbial content of the sunny classrooms (0407 and 0409) is obviously higher than night side classrooms (0408 and 0410), the total bacteria concentration of classroom 0407 is 2.1 times to classroom 0408, and the concentration of classroom 0409 is 1.5 times to classroom 0410. Among the 4 sampling points, the highest total concentration of air microorganisms is 8.0×102 cfu·m-3, the lowest value is 3.0×102 cfu·m-3, and the average value is 6.0×102 cfu·m-3. Bacteria as the main air microorganism in summer campus classroom, accounted 77.4%, mold accounted18.9%, actinomycetes accounted 3.7%. According to the air microorganism standards, it evaluates that each time of indoor air is clean.
- Published
- 2013
44. Development of That Health-Related Fitness Management System for Civil Servants That Based on Web
- Author
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Shi Luo, Bin Wang, San Ming Liu, Hong Yu Ma, and Tian You Jiang
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Engineering ,Health (social science) ,General Computer Science ,Computer science ,media_common.quotation_subject ,General Mathematics ,Civil engineering ,Civil servants ,Education ,Transport engineering ,Software ,State (polity) ,Set (psychology) ,media_common ,General Environmental Science ,Structure (mathematical logic) ,Government ,business.industry ,General Engineering ,Health related ,General Medicine ,Public relations ,Psychological evaluation ,Test (assessment) ,Engineering management ,General Energy ,Work (electrical) ,Management system ,business - Abstract
Civil servants of the state, working in the government institutions of various levels and executing the mission of state administrative functions and powers. It is true that civil servants need to own healthy physique (i.e. physical, psychological and social adaptation) to guarantee them competent in the posts. However, the properties and characteristics of civil servants work lead to their health-related fitness problems. This paper addresses the computer science and psychology integrated into the psychological health education and tries to explore and study the features of civil servants health. According to the needs of physical health construction of civil servants, we develop health evaluation system for civil servants. This article introduces this system in terms of the general structure of system overview, system modules classification and characteristics of the system. The system using asp.net, SQL Server software to complete the civil servants' physical health evaluation system so as to realize the civil servants' health-related fitness of the measurement of computerized. This testing system can be directly connected to the database and the test result confirms that this is a set of standardized, easy-expand applying psychological evaluation system.
- Published
- 2013
45. Psychological and Behavioral Characteristics of Problem Gamblers
- Author
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Yu-Xuan Cai, Hong-Yu Ma, Wen-Wen Shi, Bin Wang, and Xiao-Bing Luo
- Subjects
General Economics, Econometrics and Finance - Published
- 2013
46. End-to-end utility-based resource allocation for multi-RAT nodes in heterogeneous cognitive wireless networks
- Author
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Kai Niu, Feng Chen, Hong-yu Ma, Wen-jun Xu, and Jiaru Lin
- Subjects
Cognitive wireless network ,Computer Networks and Communications ,Computer science ,business.industry ,Network packet ,Iterative method ,Node (networking) ,Power (physics) ,Cognitive radio ,End-to-end principle ,Signal Processing ,Resource allocation ,business ,Information Systems ,Computer network - Abstract
This paper studies the problem of effective resource allocation for multi-radio access technologies (Multi-RAT) nodes in heterogeneous cognitive wireless networks (HCWNs). End-to-end utility, which is defined as the delay of end-to-end communication, is taken into account in this paper. In the scenario of HCWNs, it is assumed that the cognitive radio nodes have the ability of Multi-RAT and can communicate with each other through different paths simultaneously by splitting the arrival packets. In this paper, the problem is formulated as the optimization of split ratio and power allocation of the source cognitive radio node to minimize the delay of end-to-end communication, and a low complexity step-by-step iterative algorithm is proposed. Numerical results show good performance of the proposed algorithm over two other conventional algorithms.
- Published
- 2013
47. Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection
- Author
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Hong-Yu Ma, Ying Huang, Ai-Sheng Xiong, Wei Huang, Zhi-Sheng Xu, and Feng Wang
- Subjects
Proteomics ,0301 basic medicine ,TYLCV ,Plant Science ,Biology ,Plant disease resistance ,Tomato ,Virus ,law.invention ,03 medical and health sciences ,Solanum lycopersicum ,law ,Plant virus ,Electrophoresis, Gel, Two-Dimensional ,Expression pattern ,Tomato yellow leaf curl virus ,Cultivar ,Polymerase chain reaction ,Plant Diseases ,Genetics ,business.industry ,fungi ,Begomovirus ,Proteomic approach ,food and beverages ,2-DE ,biology.organism_classification ,Biotechnology ,030104 developmental biology ,Leaf curl ,business ,Research Article - Abstract
Background Tomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. The virus is a widespread plant virus that causes important economic losses in tomatoes. Genetic engineering strategies have increasingly been adopted to improve the resistance of tomatoes to TYLCV. Results In this study, a proteomic approach was used to investigate the molecular mechanisms involved in tomato leaf defense against TYLCV infection. Proteins extracted from leaves of resistant tomato cultivar ‘Zheza-301’ and susceptible cultivar ‘Jinpeng-1’ after TYLCV infection were analyzed using two-dimensional gel electrophoresis. Eighty-six differentially expressed proteins were identified and classified into seven groups based on their functions. For several of the proteins, including CDC48, CHI and HSC70, expression patterns measured using quantitative real-time PCR differed from the results of the proteomic analysis. A putative interaction network between tomato leaves and TYLCV infection provides us with important information about the cellular activities that are involved in the response to TYLCV infection. Conclusions We conducted a comparative proteomic study of TYLCV infection in resistant and susceptible tomato cultivars. The proteins identified in our work show a variety of functions and expression patterns in the process of tomato–TYLCV interaction, and these results contribute to our understanding of the mechanism underlying TYLCV resistance in tomatoes at the protein level. Electronic supplementary material The online version of this article (doi:10.1186/s12870-016-0819-z) contains supplementary material, which is available to authorized users.
- Published
- 2016
48. Prevalence and burden of Toxoplasma gondii infection in HIV-infected people: a systematic review and meta-analysis
- Author
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Zedong Wang, Hong-Yu Ma, Huan-Huan Liu, Feng Wei, Quan Liu, Zhongyu Li, Xing-Quan Zhu, and Shuchao Wang
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Adolescent ,Epidemiology ,030106 microbiology ,030231 tropical medicine ,Immunology ,Population ,HIV Infections ,03 medical and health sciences ,0302 clinical medicine ,Web of knowledge ,Cost of Illness ,Virology ,Environmental health ,Hiv infected ,parasitic diseases ,Prevalence ,Medicine ,Humans ,education ,Africa South of the Sahara ,education.field_of_study ,biology ,business.industry ,Coinfection ,Toxoplasma gondii ,Odds ratio ,biology.organism_classification ,Infectious Diseases ,Increased risk ,Latin America ,Caribbean Region ,Meta-analysis ,Female ,business ,Toxoplasma ,Toxoplasmosis - Abstract
Summary Background Worldwide, 30% of the world's population have antibodies to the intracellular protozoan parasite Toxoplasma gondii and about 36·7 million people are infected with HIV, but little is known about the prevalence of co-infection with T gondii and HIV. We aimed to characterise the epidemiology and burden of T gondii co-infection in people with HIV infection. Methods In this systematic review and meta-analysis, we searched PubMed, Embase, Google Scholar, ScienceDirect, Chinese Web of Knowledge, Wanfang, and Chongqing VIP databases for studies reporting T gondii infection in HIV-infected people from inception to Feb 29, 2016. Studies were included if they investigated people with HIV infection and presented data that allowed us to establish the prevalence of T gondii infection. We excluded reviews, repeated studies, or animal studies, as well as studies that provided the final results without raw data, had a sample size less than 30 people, had unclear diagnostic methods of T gondii infection, or that included populations with increased risk of T gondii infection. We extracted the numbers of patients with HIV infection and T gondii co-infection from the identified studies. We estimated pooled prevalence of T gondii infection in HIV-infected people by a random-effects model, and evaluated its overall infection burden worldwide. Findings Our search identified 7843 records and after removal of duplicates and initial screening, we reviewed 312 studies in full. Of these articles, 238 were excluded, leaving 74 studies that included 25 989 HIV-infected people from 34 countries. Of these people, 7326 had T gondii co-infection and we estimated the pooled worldwide prevalence of T gondii infection to be 35·8% (95% CI 30·8–40·7). 2353 of 8837 of people in Asia and the Pacific had co-infection with T gondii and HIV (prevalence 25·1%, 95% CI 19·0–31·2), and prevalence was low in this region compared with that in sub-Saharan Africa (44·9%, 32·3–57·5, 2129/5686; odds ratio [OR] 0·61), Latin America and the Caribbean (49·1%, 27·9–70·4, 510/980; OR 0·33), and North Africa and the Middle East (60·7%, 24·1–97·3, 245/439; OR 0·29). 1561 of 3780 people in low-income countries had co-infection (54·7%, 95% CI 35·8–73·5), which was higher than in middle-income countries (34·2%, 27·4–40·9, 3632/11 540; OR 1·53) and high-income countries (26·3%, 20·4–32·2, 2133/10 669; OR 2·82). Worldwide, we calculated that there were roughly 13 138 600 (95% CI 11 303 600–14 936 900) cases of T gondii co-infection in HIV-infected people, with 87·1% in sub-Saharan Africa (11 449 500 cases, 95% CI 8 236 500–14 662 500). Interpretation Our findings suggest that people with HIV infection have a very high burden of T gondii infection, especially in sub-Saharan Africa, and emphasise the importance of routine surveillance for T gondii infection in all HIV-infected people. Funding National Natural Science Foundation and the Special Fund for Agro-scientific Research in the Public Interest in China.
- Published
- 2016
49. Administration of SB239063, a potent p38 MAPK inhibitor, alleviates acute lung injury induced by intestinal ischemia reperfusion in rats associated with AQP4 downregulation
- Author
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Hong-Yu Ma, Zheng Deng, Ting-Hua Wang, Yun-Hui Zhang, Yan-yan Xu, Rui-ai Yang, Liu-Lin Xiong, Yan-Xia Qin, Yan Tan, Ping Dai, Wei Zhao, and Qin-Jie Xia
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Male ,Pathology ,medicine.medical_specialty ,Immunology ,Acute Lung Injury ,Inflammation ,Pharmacology ,Lung injury ,p38 Mitogen-Activated Protein Kinases ,Rats, Sprague-Dawley ,03 medical and health sciences ,Edema ,medicine ,Immunology and Allergy ,Animals ,Humans ,Molecular Targeted Therapy ,Lung ,Aquaporin 4 ,business.industry ,Imidazoles ,respiratory system ,Pulmonary edema ,medicine.disease ,respiratory tract diseases ,Mesenteric Arteries ,Rats ,Intestines ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Pyrimidines ,Reperfusion Injury ,Tumor necrosis factor alpha ,medicine.symptom ,business - Abstract
Acute lung injury (ALI), induced by intestinal ischemia reperfusion (II/R) injury, is characterized by pulmonary edema and inflammation. Aquaporin 4 (AQP4), has been pointed out recently involving in edema development. Previous studies have shown that p38 mitogen activated protein kinase (MAPK) activation resulted in lung inflammation, while p38 MAPK inhibitor can alleviate the pathology injury of lung tissue. However, the regulated mechanism of p38 MAPK in ALI induced by II/R is unclear. In this study, we established II/R rats' model by clamping the superior mesenteric artery (SMA) and coeliac artery (CA) for 40min and subsequent reperfusion for 16h, 24h, 48h. Subsequently, SB239063, a specific inhibitor of the activity of p38 MAPK, was injected (10mg/kg) intraperitoneally 60min before the operation. The severity of ALI was determined by histology analysis (HE staining and ALI scoring) and lung edema (lung wet/dry weight ratio) assessment. Western blot (WB) was applied to detect the expression level of AQP4 and phosphorylated (P)-p38 MAPK, and the localization of AQP4 was detected by immunofluorescent staining (IF). We found that AQP4 could express in the lung tissue. II/R could significantly induce lung injury, confirmed by lung injury scores and lung wet/dry weight ratios. The level of P-p38 MAPK and AQP4 were largely up-regulated in lung tissues. Moreover, inhibition of p38 MAPK activity could effectively down-regulate AQP4 expression and diminish the severity of II/R-induced ALI. These novel findings suggest that inhibition of p38 MAPK function should be a potential strategy for the prevention or treatment of ALI, by targeting AQP4 in future clinic trial.
- Published
- 2016
50. The Study of Humidity Effect on Self-Heated Surface Micromachined Polysilicon Resistor
- Author
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Hong Yu Ma, Weidong Zhang, and En Jie Ding
- Subjects
Thermal sensors ,Materials science ,business.industry ,Mechanical Engineering ,Thermal resistance ,Humidity ,law.invention ,Thermal conductivity ,Mechanics of Materials ,law ,Electronic engineering ,Microelectronics ,General Materials Science ,Relative humidity ,Resistor ,Composite material ,business - Abstract
Polysilicon is a very attractive material in microelectronics for thermal sensors. In this paper, the effect of humidity on thermal resistance of surface micromachined polysilicon is invested. It is found that the thermal conductivity variation of air due to humidity changes the suspended polysilicon resistor evidently. This property could be used as the working principle of relative humidity sensor.
- Published
- 2011
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