Search

The aptamer TY04 is a single-stranded DNA. However, its biological function has not been elucidated. Here, we found that TY04 specifically bound to multiple myeloma cells MM.1S, and some membrane proteins on the surface of MM.1S cells constituted the target molecules of TY04. TY04 inhibited the growth of multiple myeloma cell lines, induced cell cycle arrest in mitosis, and resulted in a significant accumulation of binucleated cells. Following TY04 treatment, a concomitant increase in CDK1 and cyclin B1 expression occurred. In addition, TY04 treatment also resulted in a significant downregulation of γ-tubulin. Considering the unique advantages of aptamers, TY04 shows great potential as a drug candidate to treat multiple myeloma.

In this paper, introduces the ideal of green design and the technology of green manufacture in the process of ship building; analyzes the factors those impact the implement of the ideal of green design and the technology in the process of ship building. Puts forward some advises about how to speed up the progress of green design and manufacture technology in the process of shipbuilding.

Traditional critical goals for industrial products design includes five factors. They are utility, appearance, ease of maintenance, low costs and communication[1]. In this paper, puts forth a new perspective, in which considers that embodying of human-centered perspective should be also another critical goal to industrial product besides traditional five critical goals. The paper is aimed at giving rise to the industrial designers to pay more attention to the design principles of ‘Human-Centered’ during their household bisic appliances product design because they are usually ignored by the designers. In the paper, mainly analyzed some household basic appliances products, which are used in every day life of people. The way of study is to analyze some problems in the products design by the examples of the products that are being applied in every day life, but there are some defects. What the analyses mainly concerned is how to embody the principle of “human-centered” in industrial product design. The “green design” and “healthy design” should be put the first place when industrial designers to design industrial products.

Based on micro-jet spraying technology, by spouting fluidized bed, aquatic feed encapsulating mechanism and experimental process were introduced, experimental conditions and parameters were discussed.Granular aquatic compound feed encapsulating experiments were done by spouting fluidized bed, the relationship between membrane thickness and microencapsulated time was analysed, stability indices of microencapsulated feed in the water was determined, the test results show that based on micro-jet spraying technology, feed encapsulating experiment parameters controlled easily, microencapsulated feed in smooth shape and good stability, the loss rate of microencapsulated feed in dissolution is less than that of unmicroencapsulated feed, and the spouting fluidized bed in low cost and power consumption , handled easily , so has great promotional value.

Metallothionein (MT) imprinted TiO2 membrane was synthesized via surface sol–gel process, using MT as template and TiO2 sol as imprinted matrix. Appropriate template cavities in the TiO2 sol–gel membrane were formed after the template molecules were removed on treatment with 1% NaOH solution. In situ technique of quartz crystal microbalance (QCM) was employed to study the molecular imprinting behavior of MT on nanocrystalline titania membranes. The imprinted membrane showed selectivity recognition for MT as compared to the other proteins. The amount of adsorption increased with the increasing of MT concentration both on imprinted membrane and non-imprinted membrane. The adsorption amount increased with the increasing of pH on imprinted membrane.

Multiple myeloma (MM), also known as plasma cell myeloma, is characterized by accumulation of clonal plasma cells in the bone marrow and overproduction of monoclonal immunoglobulin (Ig) in the blood or urine. MM accounts for approximately 10% of all hematologic malignancies. Despite recent advances in the understanding and treatment of this disease, MM remains an incurable disease in the vast majority. With conventional chemotherapy, the 5-year median survival rate for MM patients is approximately 25%. Aptamers are single-stranded RNA or DNA sequences that bind to target molecules with high affinity and specificity. Compared with antibodies, aptamers have unique advantages including easy chemical synthesis and modification, low toxicity, lack of immunogenicity, and rapid tissue penetration, Based on these advantages, aptamers show great potential for therapeutic application. The aptamer TY04 is a single-stranded DNA (ssDNA) generated by a method named cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX), We found TY04 strongly inhibited the growth of multiple myeloma cell lines including MM1.S, NCI-H929, KM3 and OPM2,The concentration of TY04 to inhibit 50% cell growth (IC50) on MM1.S was 3.89 μM. In contrast, TY04 had no effect on the growth of non-tumor cell lines — immortal B lymphoblastoid cell lines. Next, we used MM1.S cell line as the model to study the mechanism of TY04 anti- multiple myeloma. Flow cytometry analysis showed that TY04 with the sequence specifically bind to MM1.S cells when compared with unselected ssDNA library control. To investigate whether the target molecules of TY04 are membrane proteins on cell surface, MM1.S cells were treated with trypsin and proteinase k for 2 or 10 minutes before incubation with TY04. The result revealed that TY04 lost partly recognition ability on treated cells, indicating that the target molecules were most likely membrane proteins. Furthermore, we evaluated the cell cycle distribution of MM1.S after TY04 treatment. We found that TY04 significantly caused cell-cycle arrest in G2/M phase. The percentage of G2/M phase cells increased from 30.1±1.56 to 53.2±6.36. To identify the underlying molecular mechanism, G2/M-related proteins were assayed by flow cytometry. Following TY04 treatment, a concomitant inhibition of ERK1/2, cyclin B, CDK1 and γ-tubulin expression occurred. Meanwhile, human cell cycle PCR array was used to analyze the expression of 84 genes key to cell cycle regulation in TY04-treated MM1.S cells. Our results indicated that aptamer TY04 decreased the genes expression of CCNB1, CCNB2, BIRC5, BRCA1 and CCNH, which were involved in the progress of G2/M phase. All these results are significant in that they provide a framework for further exploring the use of TY04 as a novel anti-multiple myeloma agent. Disclosures: No relevant conflicts of interest to declare.