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1. Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses

Author

Sirc J, Kubinova S, Hobzova R, Stranska D, Kozlik P, Bosakova Z, Marekova D, Holan V, Sykova E, and Michalek J

Subjects
Medicine (General), R5-920
Abstract

Jakub Sirc,1 Sarka Kubinova,2 Radka Hobzova,1 Denisa Stranska,3 Petr Kozlik,4 Zuzana Bosakova,4 Dana Marekova,2 Vladimir Holan,5 Eva Sykova,2 Jiri Michalek11Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic; 2Department of Neuroscience, Institute of Experimental Medicine, Academy of Science of the Czech Republic, Prague, Czech Republic; 3Elmarco Ltd, Liberec, Czech Republic; 4Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Prague, Czech Republic; 5Department of Transplant Immunology, Institute of Molecular Genetics, Academy of Science of the Czech Republic, Prague, Czech RepublicAbstract: Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.Keywords: nanofibers, electrospinning, multilayered structure, morphology, gentamicin, drug release

Published
2012

12. сОДЕРжАНИЕ РАБОт, ОпУБлИкОВАННых тОлькО В ЧЕшскОМ ИжДАНИИ НАстОьЩЕгО жУРНАлА

Author

HollÄnder, E. F., Srnka, D., Jech, Č., Lehraus, I., Mokrý, P., Slavík, B., Wooster, W. A., SkŘivánek, J., Veselá, M., Karel, F., Gála, J., Tichý, J., Zelenka, J., Kvasnica, J., Nutting, J., Libovický, S., Vacek, K., Antončík, E., Eckertová, L., Váňa, J., Pekárek, L., Pátý, L., Pohanka, J., Dohnálek, J., Frič, V., Hlubuček, V., Holan, V., ďatlov, J., Bartoš, H., Plecitý, M., Mareš, P., Hábovčík, P., Veis, š., Láska, S., Pekárek, L., Krpata, V., Novák, M., šícha, M., Bakule, R., šícha, M., Veselý, V., Kracík, J., ďurček, J., Kracík, J., Kocian, P., Gross, B., Truneček, V., Hernoch, V., Hix, A., Eckertová, L., Kryška, L., Eckertová, L., Stratonov, O., Jedlička, M., Tomková, E., VyslouŽil, A., Zikeš, V., Schneider, P., Vaško, A., Jedlička, M., štoksa, L., Prchlík, J., GadŽuk, B., Beránek, I., Holý, B., Ladman, R., Pátý, L., NeuŽil, M., Eckertová, L., Kryška, L., Pátý, L., Schürer, P., Veis, š., Schürer, P., Janatka, M., Urgošík, B., Neuman, J., Bláha, A., Pátý, L., Veis, š., Neuman, J., Bláha, A., PŘibyl, F., Eröss, E., ŽiŽka, E., Marton, K., Harman, R., RůŽička, J., Pátý, L., Plichta, L., šípek, L., štícha, M., Vejvodová, J., Teichman, J., Rytina, K., Seidl, M., Klíma, R., Rohlíček, F., Jareš, V., Lom, T., Koziak, R., Podskalský, E., Pecha, V., Vojtášek, S., Horna, J., Zima, V., čičmanec, P., šurka, š., Mack, Z., Szánto, L., and Kunzl, V.

Published
1961
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14. The effect of amphetamine sensitization on mouse immunoreactivity

Author

Kubera, M., Filip, M., Basta-Kaim, A., Nowak, E., Bogusława Budziszewska, Tetich, M., Holan, V., Korzeniak, B., and Przegalinski, E.

Subjects
locomotor hyperactivity, amphetamine sensitization, production of IL-4, splenocytes proliferation
Published
2002

15. Urocanic acid enhances IL-10 production in activated CD4+ T cells

Author

Holan, V., Kuffova, L., Zajicova, A., Magdalena Krulova, Filipec, M., Holler, P., and Jancarek, A.

Subjects
CD4-Positive T-Lymphocytes, Male, Antigen Presentation, Mice, Inbred BALB C, Immunology, Urocanic Acid, Stereoisomerism, Lymphocyte Activation, Interleukin-10, Mice, Inbred C57BL, Mice, Adjuvants, Immunologic, Gene Expression Regulation, T-Lymphocyte Subsets, Immunology and Allergy, Animals, Female, Spleen
Abstract

The immunosuppressive effects of UV radiation have been well documented. This suppression has been attributed to the action of the cis form of urocanic acid (UCA), a photoproduct of trans-UCA, a natural constituent of the skin. Here, we show that mouse spleen cells preincubated with cis-UCA have a diminished proliferative response to allogeneic cells in MLC and to stimulation with anti-CD3 mAb. Cells preincubated with cis-UCA also had a decreased ability to serve as APC and to stimulate the proliferation of allogeneic lymphocytes in MLC. Simultaneously, the production of IL-2 and IFN-γ by cells preincubated with cis-UCA was decreased. However, IL-10 gene expression and IL-10 protein secretion by spleen cells stimulated in the presence of cis-UCA were significantly enhanced. The principal cell population displaying the cis-UCA-induced elevated production of IL-10 was CD4+ T cells, which were shown to be a direct target of cis-UCA action. This was also supported by the observation that production of IL-10 by stimulated splenic non-T cells or by macrophages was not altered by cis-UCA. The enhanced production of IL-10 by activated CD4+ T cells may represent a novel pathway of UVB radiation-induced, cis-UCA-mediated immunosuppression. We suggest that the elevated production of IL-10 by activated CD4+ T cells may account for the suppressor T cell phenomena described in UV-irradiated recipients.

Published
1998

25. Development of Unique House Mouse Resources Suitable for Evolutionary Studies of Speciation

Author

Pialek, J., primary, Vyskocilova, M., additional, Bimova, B., additional, Havelkova, D., additional, Pialkova, J., additional, Dufkova, P., additional, Bencova, V., additional, Dureje, L., additional, Albrecht, T., additional, Hauffe, H. C., additional, Macholan, M., additional, Munclinger, P., additional, Storchova, R., additional, Zajicova, A., additional, Holan, V., additional, Gregorova, S., additional, and Forejt, J., additional

Published
2007
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27. Evaluation of corneal graft rejection in a mouse model. (Perspective)

Author

Plskova, J., Kuffova, L., Holan, V., Filipec, M., and Forrester, J.V.

Subjects
Cell proliferation -- Physiological aspects, Cornea -- Physiological aspects, Endothelium -- Physiological aspects, Graft rejection -- Physiological aspects, Surgery -- Physiological aspects, Health, Physiological aspects
Abstract

Corneal graft rejection presents clinically and in experimental models as opacification and is considered to be the result of endothelial cell dysfunction or loss. However, recovery from opacification can occur [...]

Published
2002

37. Quantitative evaluation of the corneal endothelium in the mouse after grafting.

Author

Piskova, J., Kuffova, L., Filipec, M., Holan, V., and Forrester, J. V.

Subjects
EPITHELIUM, SKIN grafting, HOMOGRAFTS, TISSUES, TRANSPLANTATION of organs, tissues, etc., SYMPTOMS
Abstract

Background/aim: Corneal graft survival depends critically on the quality of the endothelium. In this study the authors aimed to evaluate corneal endothelium in mice at different times after transplantation and to correlate endothelial integrity with corned graft survival. Methods: Syngeneic and allogeneic corneal grafts at various times (days 0-60) after engraftment were examined in flat mount preparation by confocal microscopy, by evaluating the hexagonal pattern of the endothelial monolayer using actin staining of the cell cortex. Corneas from untreated mice and from mice, who were grafted after removal of draining lymph nodes served as controls. Results: In control corneas, more than 90% of the posterior surface was covered by endothelium. Syngeneic grafts were always covered by 54-99% of endothelium. In contrast, the posterior surface of corneal allografts showed great variation in the degree of endothelial cell coverage (0-98%). In addition, clinical opacity grading measure was not a reliable predictor of endothelial coverage. Conclusion: In corneal allografts there is progressive loss of endothelium over time, unlike with syngeneic grafts. However, in the early stages of allograft rejection, the grade of graft opacity does not accurately reflect the degree of endothelial cell coverage. Although corneal opacity grade is considered the main determinant of graft rejection, the data suggest that both the grade of corneal opacity plus a sufficient post- graft time duration (>8 weeks in the mouse) are required for the diagnosis of irreversible graft rejection. [ABSTRACT FROM AUTHOR]

Published
2004
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