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Background: Melanoma is the third most common type of skin cancer in Mexico and represents 75% of skin cancer deaths. Dermoscopy is a diagnostic tool that increases early detection of melanoma compared to naked eye examination. Objective: The aim of this study was to describe the clinical, dermoscopic and histological characteristics of patients with a confirmed diagnosis of cutaneous melanoma treated at the "La Raza" National Medical Center. Material and methods: A retrospective, descriptive and cross-sectional study was carried out from March 1998 to December 2013, with 187 histologically confirmed cases, considering: sex, age, skin phototype, history, topography of the lesion, dermoscopic pattern, metastasis at the time of diagnosis and histological subtype, Breslow index and Clark index, using the chi-square test as a non-parametric statistical method to analyze the data obtained. Results: Most patients had skin phototype III and the most affected location was the lower limb. Clinically, acral lentiginous melanomas and nodular melanomas were the most observed. The most common dermoscopic finding was the multicomponent pattern. Clinically and histologically, the most frequent subtype associated with metastasis was nodular melanoma. Acral lentiginous melanoma was more common among patients without metastasis. Conclusions: Unfortunately, melanoma is still diagnosed in advanced stages in Mexico; for its early recognition, training in the use of dermoscopy and greater awareness about melanoma in the Mexican population must be encouraged. [ABSTRACT FROM AUTHOR]

La histología, como disciplina fundamental en ciencias de la salud, desentraña la complejidad microscópica de los tejidos y órganos, proporcionando la base necesaria para comprender los procesos fisiológicos y patológicos del cuerpo humano. En un mundo marcado por la digitalización, la integración de entornos virtuales en la enseñanza emerge como una herramienta revolucionaria. Este ensayo explora la vanguardia de la educación en histología, destacando la importancia de la innovación mediante entornos virtuales para potenciar la formación en ciencias de la salud. Al fusionar la riqueza conceptual de la histología con la tecnología digital, se despliega un terreno educativo que no solo mejora la comprensión, sino que redefine la manera en que los futuros profesionales de la salud interactúan con el conocimiento anatómico y fisiológico.

This study aimed to investigate the morphological and morphometric features, as well as the histological composition of the medial and lateral menisci of domestic cats. A total of 180 menisci were obtained from 45 skeletally mature cat cadavers, followed by measuring the peripheral extension, width and thickness, circumference of the body and the body with horns, articulating height, and superior articulating length. A histological assessment was conducted to determine the meniscal cellularity and histomorphology. Each meniscus was evaluated in three regions (cranial horn, body, and caudal horn) and three zones (outer, middle, and deep). The proteoglycan content and collagen distribution were also examined. Overall, 62.2% of the medial meniscus displayed a crescentic shape, whereas 86.7% of the lateral meniscus exhibited a C-shaped configuration. The morphometric analysis revealed that the lateral menisci significantly differed (P<0.05) in circumference, thickness, width, and articulating height from the medial menisci. Despite cell density was not significantly different across the regions, the cellularity decreased from the outer zone to the deep zone of the menisci. Proteoglycan content was not significantly different in the various regions but was increased significantly (P<0.05) in the middle meniscal zone. Types I, II, and III collagens were distributed throughout the meniscal regions and zones. Type I collagen was abundant within the meniscus. In contrast, type III collagen is primarily localized in the middle zone. In conclusion, feline menisci exhibit morphometric variations and distinctive histological features, offering crucial insights for veterinarians and researchers. [ABSTRACT FROM AUTHOR]

RESUMO Introdução: Histologia é o estudo das células e dos tecidos biológicos que compõem os seres vivos. Apesar de sua fundamental importância, a histologia ainda é um campo de difícil compreensão para uma parcela considerável dos estudantes, e, por consequência, a aprendizagem de outras ciências correlatas também fica prejudicada. Objetivo: O presente estudo se dispôs a elaborar materiais didáticos de histologia, utilizá-los como facilitadores do processo ensino-aprendizagem e avaliar a percepção dos discentes da Universidade Federal de Santa Catarina acerca das ferramentas desenvolvidas. Método: Os materiais didáticos elaborados foram: apostila, banco de imagens, banco de casos clínicos e banco de mapas mentais. Para elaborá-los, utilizou-se o software Microsoft PowerPoint 2019. A exceção a essa forma de criação se deu somente com o banco de mapas mentais, o qual foi produzido com o aplicativo de mapeamento mental MindMeisteir. Distribuíram-se os materiais aos discentes que avaliaram suas contribuições para o aprendizado em histologia por meio de um questionário anônimo no formato Likert. Os dados foram compilados em tabelas utilizando o Microsoft Excel 2019 e analisados por meio de estatística descritiva. Resultado: Os materiais desenvolvidos pelo projeto foram avaliados majoritariamente como “bons”. Quanto à frequência de uso dos materiais, obteve-se as seguintes respostas: “quase sempre” ou “sempre”. A tendência da maioria dos voluntários foi concordar completamente, para todos os documentos avaliados, de que houve um impacto positivo do material no entendimento de histologia. Conclusão: O desenvolvimento de materiais pelo projeto “Histologia sem Artefato” auxiliou na construção de conhecimento dos alunos na grande área de histologia.

One of the most important figures in the history of neurohistology, Giuseppe Levi (1872–1965) contributed in numerous ways to neuroscience, particularly in the fields of neuronal plasticity and the understanding of sensory ganglia. His daughter Natalia Ginzburg, née Levi (1916–1991), on the other hand, achieved fame as one of the most celebrated Italian writers of the twentieth century. Lessico Famigliare (Family Lexicon), from 1963, is a semibiographical account of her life in which she describes the life and character of her father in detail, providing depth and complexity to a seminal figures in the development of neuroscience. A thorough reading of the book enables modern neurologists to fully appreciate Levi's life and contributions, by means of humanizing him and giving context to his life and works. The present article provides a summary of Levi's and Natalia's lives and times as well as an analysis of the book and of the intimate, vivid descriptions of the neurohistologist's life.

Background and aims: The false-root knot nematode (Nacobbus spp.) comprises polyphagous species, with pepper (Capsicum annuum) being one of the most affected crops. Due to the lack of resistant pepper genotypes, we compared the responses of different commercial cultivars to identify possible plant defence mechanisms against N. celatus. M&M: Nematodes from Río Cuarto (Córdoba Province) and Lisandro Olmos (Buenos Aires Province) were inoculated on pepper cultivars: California Wonder (control), Fyuco INTA, Yatasto, and Fenomeno RZ. Plants were grown under a greenhouse and galls were analysed by histopathological techniques. Results: The roots of the commercial cultivars showed the typical morpho-anatomical alterations induced by N. celatus: gall formation, hyperplasia reactions, development of syncytia in the central cylinder and disorganisation of vascular tissues. Syncytial cells in contact with females of nematodes from Lisandro Olmos showed a defence reaction by Fyuco INTA, whereas feeding sites in Fenomeno RZ did not denote high metabolic activity in nematodes of both origins. Conclusions: The different responses observed between populations and/or hosts demonstrate the importance of considering nematodes from different geographical origins when testing plant material for tolerance and/or resistance to N. celatus. Studies of this nature are necessary since they facilitate a more in-depth understanding of the parasite-plant interaction. [ABSTRACT FROM AUTHOR]

Copyright of Lilloa is the property of Fundacion Miguel Lillo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Histology is considered one of the most important but challenging topics in health sciences. Deficiencies in teaching and general histology directly affect the student's understanding, performance and promote dynamism and integration while covering the primary curriculum. The goal of the current study was to assess and provide a better experience of difficulties in learning histology, examine some standard teaching strategies, and determine how to implement suitable changes to improve the curriculum from students' perspectives. The study comprised of a self-administered questionnaire that included descriptive questions. Data was collected based on the survey conducted via 100 students studying histology. Data was analyzed further qualitatively and quantitatively to elaborate on the difficulties in this area. Many of them strongly agreed that the lack of essential comprehension regarding using light and electron microscopes resulted in challenges with comprehending and identifying tissue samples for image identification due to inadequate anatomical knowledge. Moreover, according to students, some difficulties understood the topic, perception of new terminologies, and insufficient teaching strategies to grasp students' interests. They also agreed that improvement was needed in terms of the modes of teaching employed by histology teachers; they felt that modern education techniques based on practical tasks should be incorporated to stimulate student interest and make understanding histological concepts easier. The Institution should modify the teaching system to allocate more time to relevant subjects to make this subject matter more interesting. This survey-based study evaluated that students faced some challenges while studying the current curriculum of histology. From the student's perspective, it is identified that some changes are needed to improve the course curriculum and way of teaching to make it more understandable. [ABSTRACT FROM AUTHOR]

The study of medicine begins with the study of the macroscopic and microscopic structure of the human body, which has evolved over the years. In 2019 with the Covid-19 pandemic, education changed from the traditional in-person to virtual modality, which implied a series of changes for teachers and students. In the present work, a questionnaire was administered to 123 medical students at the Catholic University of Santiago of Guayaquil in 2023 about the teaching of anatomy and histology during and after the pandemic. Among the results, 43.1% prefer face-to-face evaluations, 86.2% state that the teacher-student relationship is better in person, virtual education generates demotivation in 57.7%. In theoretical anatomy and histology, it is preferred that the teacher use all the teaching resources available, as well as in practical anatomy, and in practical histology, however, the use of a microscope is preferred by 45.5%. For this reason, face-to-face education is preferred both in theory and in practice of anatomy and histology, being flexible in the pedagogical resources of theory, but in practice, especially in histology, the student prefers conventional teaching. In conclusion, the study of basic medical subjects should be done in person whenever possible, and use the virtual modality in special cases. [ABSTRACT FROM AUTHOR]

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Copyright of Caldasia is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introducción: la Histología no siempre tuvo el lugar que ocupa hoy en los planes de estudio de la carrera de Medicina, su enseñanza se ha modificado en el transcurso de las décadas con el fin de adaptarse a las necesidades del perfil del egresado. Objetivo: fundamentar los elementos teóricos relacionados con el origen y evolución de la enseñanza de la Histología, así como sus tendencias en el contexto educativo moderno. Métodos: se realizó una revisión bibliográfica a través de las bases de datos SciELO, MEDLINE, y el motor de búsqueda Google académico. Las palabras claves utilizadas fueron: enseñanza de la Histología, tendencias en la enseñanza de la Histología, historia de la Histología. Se recuperaron 50 publicaciones, fueron seleccionadas 24 ajustadas a la temática y el objetivo propuesto. Desarrollo: se delimitaron tres núcleos temáticos: el origen de la Histología e inicio de su enseñanza, evolución en Cuba y el mundo, hasta llegar a las tendencias actuales en la enseñanza de esta ciencia, incluyendo metodologías y recursos tecnológicos utilizados en el contexto educativo contemporáneo. Además, los autores emitieron criterios acerca de las posibilidades de aplicación de la microscopía virtual, la clase invertida y el aprendizaje basado en problemas en la educación médica superior cubana. Conclusiones: la enseñanza de la Histología en la educación médica superior ha evolucionado desde modelos centrados en el profesor con la ayuda de la microscopía óptica, hasta las tendencias pedagógicas actuales, centradas en el estudiante y favorecidas por las nuevas tecnologías de la informática y las comunicaciones.

Introdução: A sífilis é uma doença sexualmente transmissível identificada como um problema de saúde mundial. Seu acometimento gástrico, esofágico e/ou intestinal acontece em cerca de 1% dos casos. As descrições das lesões por via endoscópica e biópsias no histopatológico se assemelham com outras doenças mais prevalentes, como, por exemplo, os tumores infiltrativos da mucosa, linfomas relacionados a infecção do Helicobacter pylori, doença de Chron e adenocarcinomas. Objetivo: Relatar o caso de um paciente masculino adulto com exames de imagem mimetizando neoplasia gástrica, sendo, na verdade, lesões suspeitas de sífilis, sua resolução e conduta. Relato de caso: Homem de 39 anos buscou atendimento por conta de forte epigastralgia relacionada a período pós-prandial e a jejum prolongado, sem melhora com medicação. Relatou perda ponderal e episódios de vômitos com cor escura. Ao exame físico, dor à palpação profunda em região epigástrica sem massas palpáveis e peristalse presente. Foi feita endoscopia, cujo laudo apontou gastrite endoscópica com erosões planas acentuada no antro, com mucosa erodida, cruenta, intensamente friável, e feita biópsia da mucosa, com laudo do histopatológico resultando em grande infiltrado de plasmócitos, sendo pedido VDRL/FTA-Abs por conta da prevalência de diagnósticos diferenciais. O paciente foi diagnosticado com sífilis, e foi feito tratamento com penicilina benzatina 2.400.000UI IM três doses, uma a cada sete dias, num total de 7.200.000UI, retornando em seis meses para reavaliação. Conclusão: Apesar de se tratar de um acometimento raro da doença, deve-se sempre estar atento aos possíveis diagnósticos diferenciais a fim de se evitar intervenções cirúrgicas e procedimentos desnecessários.

This is a 52-year-old Caucasian female patient, a teacher by profession, with no personal pathological history of interest, who was admitted to the intensive care unit with COVID-19 pneumonia, after several pharmacological treatments and 20 days with a severe restrictive ventilatory pattern, without improvement and finally died. Images were obtained by digital radiography and computed axial tomography of the chest, and a lung histological study was performed. The application of imaging studies allows the observation of histopathological changes for the adequate clinical identification of patients and the implementation of effective therapeutic strategies. The imaging findings that were corroborated by the histopathological study are reported. [ABSTRACT FROM AUTHOR]

The quest for aesthetic procedures is experiencing a steady increase in popularity, concomitant with the expanding array of available treatment options. Of notable interest is the burgeoning trend in the use of minimally invasive techniques. Among the various aspects of facial anatomy, the platysma stands as a pivotal element that significantly influences the aesthetic appearance of the neck region. It has garnered particular attention as a strategic focal point in various treatments geared towards enhancing the neck's visual appeal. Additionally, the versatility of the platysma extends beyond the realm of cosmetic improvements. Its functional significance is recognized in reconstructive surgical procedures, where it may be harnessed for specific maneuvers. Furthermore, the muscle serves as a critical access point for minimally invasive endoscopic surgeries in the neck region. While these developments hold great promise, it is crucial to underscore that safety should always take precedence in any medical or surgical approach. This applies equally to the neck region, which presents a complex and intricate anatomical landscape. An in-depth understanding and meticulous investigation of the platysma in all its diverse aspects are paramount to ensuring the success and safety of any procedure conducted in this region. This comprehensive review aspires to provide a contemporary understanding of the platysma, offering an in-depth analysis that encompasses its intricate anatomy, histological characteristics, and multifaceted clinical implications. By delving into these diverse dimensions, it aims to equip healthcare professionals and researchers with a robust foundation for informed decision-making and practice. [ABSTRACT FROM AUTHOR]

Mast cells (MC) are cells of the immune system that regulate cell and tissue homeostasis, are found in low numbers, have an intact plasma membrane, and a cytoplasm with a wide variety of inflammatory chemical mediators. The activation or degranulation of mast cells implies the release of these chemical mediators (interleukins, cytokines, and more), causing tissue actions ranging from the activation of metalloproteinases to the development of anaphylactic hypersensitivity of different degrees, alterations in vascular permeability, and loss of cell homeostasis. This behavior would allow them to act as sentinels responding to pathophysiological processes. During the COVID-19 pandemic, in positive human patients, the available literature reports the presence and degranulation of mast cells in a generalized manner, especially in the respiratory tract. This study aimed to analyze the emerging role of MCs in the pathogenesis of diseases and their projection as biological markers in the treatment of diseases or pandemics. The analysis of human biopsies showed that MCs are observed as cells with diameters between 8 to 20 µm, and in inflamed tissues, degranulation of MCs is observed. The action of MCs degranulation was related to different inflammatory processes of autoimmune diseases. It is concluded that the potential of MC as therapeutic targets and biomarkers could raise new pharmacological targets, as supportive therapy, and possibly of great help in the treatment of future emerging pandemics such as the current monkeypox. [ABSTRACT FROM AUTHOR]

This study evaluated the morphology of alpacas skin. Biopsies were collected and samples were fixed in 10 % neutral buffered formalin for histological procedures. The sections were stained with hematoxylin and eosin, picrosirius red and Masson’s trichrome. Types I, III and IV collagen were analyzed by immunohistochemistry. The derma presented sebaceous and sweat glands, as well as follicular groups with medullated fibers. Type I and type IV collagen were observed at epidermis and dermis as well as in glandular structures and hair follicles. The collagen III, was observed only in dermis. [ABSTRACT FROM AUTHOR]

Introduction: Cervical cancer is the third leading cause of death from malignancies worldwide, affecting mainly low- and middle-income countries. By 2020 an incidence of 604,000 new cases was estimated. Objective: To characterize the main hospital indicators of the Cervical Cancer Early Diagnosis Program. Methods: An observational, descriptive and cross-sectional study was carried out to characterize the main hospital indicators of the Program in the Neck Pathology Service of the General Hospital Dr. Bruno Zayas Alfonso of Santiago de Cuba, from January 2020 to December 2022. The study population consisted of 443 women. The data collected were analyzed using descriptive statistic techniques, expressed in frequency and percentages. Results: Of the women studied, 60.9 %had cervical intraepithelial lesion of high degree of malignancy, with 32.6 % positive for cervical cancer. The overall percentage of useless cytological tests was 2.07 and no cells from the processing zone was 4.01, both quality indicators. There was a high significance in terms of the overall coverage rate of women at risk (K=0.615), showing that the results of the cytology largely reflect the diagnosis of histology, with a good agreement. Conclusions: Cervical-vaginal cytology remains the most valuable diagnostic method for detecting cervical intraepithelial neoplasm and early-stage carcinoma in large populations [ABSTRACT FROM AUTHOR]

The mammary glands are modified compound branched tubulo-alveolar sweat glands; whose main function is milk production. They are much more developed in women, and in men they are in a rudimentary state. Breast development begins in the 5th week of intrauterine development by an ectodermal thickening from the armpit to the groin and, by the sixth/seventh week, it condenses to a single pair of mammary ridges. At birth, the glands consist of a series of inactive ducts. At puberty, under the influence of estrogen and progesterone, acini are developed form terminal ducts. It is during pregnancy where the functional differentiation of the gland takes place to prepare the alveoli for lactation. The mammary glands are composed of 15 and 20 ducts (galactophores) that emerge from the nipple, and towards the depth of the mammary tissue they form the terminal duct-lobular unit composed of terminal ducts and acini. The ducts and acini are covered by a luminal cubic epithelial layer and a basal/myoepithelial layer. Understanding embryonic development, anatomy, physiology, and hormonal regulation of the breast is essential to understand the normal function of this organ, and to adequately interpret benign and malignant processes. [ABSTRACT FROM AUTHOR]

The guinea pig is an animal model widely used in biomedical research due to its biological similarities with humans. The objective of our study is to provide morphological support to use histological preparations of guinea pig embryos as a study model to understand the processes of human embryonic development. Our results show that guinea pig embryos present morphological characteristics similar to those observed in human embryos, suggesting that they can be used as an effective model to study human embryonic development. This finding has important implications for research and teaching using this animal model. Histological preparations of guinea pig embryos stained with hematoxylin eosin, acquired by the Autonomous University of Chile, were analyzed. Photomicrographs of histological preparations of guinea pigs at different stages of development were taken and the best images were selected to describe structures and establish estimates of embryogenesis. From the analysis of the preparations it is clear that organs such as the esophagus, spinal cord and heart present anatomical and histological similarities that make it possible to compare them with human embryonic development and gestation age in early stages. The use of guinea pig embryo preparations and their analysis from a histological aspect turns out to be a feasible methodological strategy due to the similarities in mammalian embryogenesis and the morphological concordances with the development of organs between humans and rodents. This allows this animal model to be implemented as a tool to understand human embryonic development. [ABSTRACT FROM AUTHOR]

Background. Melanoma is the malignant proliferation of melanocytes associated with aggressive behavior. The objective of this study was to determine the histological variables of cutaneous melanoma. Methods: Observational, cross-sectional, descriptive, retrospective study carried out with reports of pathologies with a diagnosis of cutaneous melanoma in a pathology laboratory in Cali between 2016-2021. The variables were age, sex, location, subtype, Breslow thickness, ulceration, margins, mitosis, lymphovascular invasion, neurotropism, tumoral regression, Clark level and tumor infiltration by lymphocytes. Results: One hundred and six reports were obtained and 54 were excluded due to duplication. A descriptive analysis was made on the 52 records that were included, the mean age was 61 years, with a higher frequency in women with 55.8%. Of the 33 cases where the histological subtype was specified, the most frequent was superficial extension with 66.6%, followed by acral lentiginous with 18.1% and nodular with 15.2%. The most frequent location was in the extremities (61.5%); the most common Breslow was IV (34.6%), and the most frequent Clark was IV (34.6%). Ulceration was in 40.4%. The nodular subtype was the most aggressive presentation where 100% presented Breslow IV. Conclusions. The most common subtype of melanoma was that of superficial extension. In our population, the second most frequent was the acral lentiginous subtype, which was always located on the extremities. More than 50% of the melanomas had Breslow greater than or equal to III, which affects the prognosis. [ABSTRACT FROM AUTHOR]

To study the gross and microscopic anatomy of the digestive system and adjoining organs (liver and pancreas) of Megaleporinus muyscorum, 25 adult specimens of both sexes, with a total length of 32.5 cm and 339 g, were euthanized. Serial 5 µm sections were obtained by paraffin technique and stained with hematoxylin-eosin. The mouth is relatively small in a subterminal position, with large flat teeth directed forwards, the gills are long, separated, and defined, the esophagus and stomach are short and muscular, and the latter is “V” shaped and low volumetric capacity. The pyloric ceca are digitiform and range between ten and 15, the intestine is relatively long, with little distention capacity and three convolutions. The liver is prominent, trilobed, and brownish, and the pancreas is diffuse. Histologically, the digestive system is composed of four layers: mucosa, submucosa, muscle, and serosa. The mucosa in the esophagus was made up of longitudinal folds and stratified flat epithelium with abundant goblet cells. The submucosa presented loose connective tissue in the three organs evaluated: the esophagus, stomach, and intestine. The muscular layer of the esophagus is made up of striated muscle with internal and external circular orientation, different from that reported for the stomach and intestine (smooth muscle with circular and longitudinal orientation). The structure of the digestive system of M. muyscorum is like that presented by other teleost, the position of the mouth and the teeth suggest that it has omnivorous feeding habits. [ABSTRACT FROM AUTHOR]

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Copyright of Klinická Mikrobiologie a Infekční Lékařství is the property of TRIOS, spol. sr.o. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Leiomyoma is a benign smooth muscle tumor that can occur in different locations; the most common is in the uterus. Lung location is very rare, and they are usually secondary to a primary tumor mainly located in the uterus. Their clinical picture varies from asymptomatic and incidental findings by imaging; even those with the most varied symptoms. They are usually bilateral and multiple, rarely presenting as single solid masses. Objective: To present a clinical case of a solitary pulmonary nodule as a presentation of a pulmonary leiomyoma. Clinical case: A 51-year-old female patient with a gynecological-obstetric history of a hysterectomy 8 years ago, who presented due to shortness of breath and chest pain. An imaging study revealed a unilateral solid mass in the right lung. Surgical intervention, type upper right pulmonary lobectomy, was decided. The histological study raised the diagnosis of a benign metastatic pulmonary leiomyoma, which was corroborated with immunohistochemical markers. Conclusions: Pulmonary leiomyoma is a rare clinical entity and constitutes a clinical, histological and imaging challenge.

Introducción: El cáncer cervicouterino ocupa el tercer lugar como causa de defunción por neoplasias malignas a nivel mundial, afectando principalmente a los países de ingresos bajos y medianos. Hacia el 2020 se estimó una incidencia de 604 000 nuevos casos. Objetivo: Caracterizar los principales indicadores hospitalarios del Programa de Diagnóstico Precoz del Cáncer Cervicouterino. Métodos: Se realizó un estudio observacional, descriptivo y transversal, que permitió caracterizar los principales indicadores hospitalarios del Programa en el Servicio de Patología de Cuello del Hospital General Dr. Juan Bruno Zayas Alfonso de Santiago de Cuba, de enero del 2020 a diciembre del 2022. La población de estudio estuvo constituida por 443 mujeres. Los datos recopilados fueron analizados mediante técnicas de estadística descriptiva, expresándose en frecuencia y porcentajes. Resultados: De las mujeres estudiadas, 60,9 % presentaron lesión intraepitelial cervical de alto grado de malignidad, con 32,6 % positivo a cáncer cervicouterino. El porcentaje global de pruebas citológicas no útiles fue de 2,07 y sin células de la zona de transformación, de 4,01; ambos indicadores de calidad. Existió una alta significación en cuanto a la tasa de cobertura global de las mujeres en riesgo (K=0,615), demostrando que los resultados de la citología reflejan en gran medida los diagnósticos de la histología, con una buena concordancia. Conclusiones: La prueba citológica cérvico-vaginal sigue siendo el método diagnóstico de mayor valor para detectar neoplasia intraepitelial cervical y carcinoma en estadio precoz en grandes masas de población.

Introducción. Históricamente se ha invertido esfuerzo en el diseño de nuevos instrumentos que superen las desventajas del estándar de referencia en cirugía, el bisturí. Como consecuencia de esto, han surgido equipos electrónicos como el electrobisturí y los diferentes dispositivos de tecnología láser. La información disponible sobre estos instrumentos sugiere que la respuesta del tejido intervenido está influenciada por los principios físicos y biológicos de corte del instrumento. Objetivo. Comparar los cambios histológicos en muestras de encía asociados al corte quirúrgico realizado con láser de diodo de 940 nm, láser de erbio, cromo: itrio-escandiogalio-granate (Er,Cr:YSGG) (2780nm) y electrobisturí mediante una presentación de serie de casos. Presentación de los casos. Se presentan tres casos de pacientes sanos sometidos a cirugía estética. El examen clínico reveló la exposición de una banda gingival queratinizada mayor de 4 mm, tejido gingival de color y textura normales, de consistencia firme y sin sangrado al sondaje periodontal. Se indicó gingivectomía con los siguientes protocolos: láser de diodo de 940 nm a 1 W, en modo continuo; láser de Er,Cr:YSGG de 2780 nm a 2,5 W, 75 Hz, modo H, aire 20, agua 40, punta de oro MT4; y bisturí eléctrico en modo de corte, a nivel de potencia cuatro. Se tomaron muestras de tejido gingival y se almacenaron en formaldehído al 10 % para su análisis histológico. Conclusión. Los tres instrumentos de corte generaron cambios histológicos producidos por el efecto térmico; los principales fueron coagulación del colágeno y carbonización.

El macrosistema Iberá presenta diversos tipos de vegetación, donde además de las acuáticas y palustres hay bosques hidrófilos, sabanas con predominio de gramíneas y praderas con especies arbustivas intercaladas entre las herbáceas. Estudios previos de anatomía foliar solo fueron realizados en plantas acuáticas y palustres. En este trabajo se estudió la anatomía foliar en especies predominantes o frecuentes que crecen en pastizales y bosques del macrosistema Iberá, con el objetivo de detectar caracteres estructurales de las hojas que puedan representar valor adaptativo a estos ambientes. Se analizaron hojas de 42 especies correspondientes a 26 familias, con microscopía óptica y electrónica de barrido. Se realizó un análisis estadístico en base a una matriz de datos, compuesta por los caracteres anatómicos y el ambiente en que crecen las especies estudiadas. Los caracteres analizados de la epidermis fueron: diseño de la cutícula, estomas, tricomas, diseño de células epidérmicas de ambas epidermis, donde se observa un predominio de idéntica morfología en ambas caras. En el mesofilo se evaluó su tipo, presencia y forma de cristales de calcio e inulina, y distribución de estructuras glandulares como laticíferos, cavidades y células secretoras. Los datos reportados son discutidos en relación a la bibliografía disponible. El análisis de agrupamiento (UPGMA) muestra que las especies que habitan en los mismos ambientes no presentan características anatómicas similares.

Introducción. El melanoma es la proliferación maligna de melanocitos asociado a un comportamiento agresivo. El objetivo de este estudio fue determinar las variables histológicas del melanoma cutáneo. Métodos. Estudio observacional retrospectivo, transversal descriptivo, realizado con reportes de patologías de pacientes con diagnóstico de melanoma cutáneo en un laboratorio de patología en Cali, Colombia, entre 2016-2021. Se incluyeron las variables edad, sexo, localización, subtipo, espesor de Breslow, ulceración, márgenes, mitosis, invasión linfovascular, neurotrofismo, regresión tumoral, nivel de Clark e infiltración tumoral por linfocitos. Resultados. Se obtuvieron 106 reportes y fueron excluidos 54 por duplicación. Se incluyeron 52 registros, la media de edad fue de 61 años, con una mayor frecuencia de mujeres (55,8 %). De los 33 casos donde se especificó el subtipo histológico, el más frecuente fue el de extensión superficial (66,6 %), seguido del acral lentiginoso (18,1 %) y nodular con (15,2 %). La localización más frecuente fue en extremidades (61,5 %). El espesor de Breslow más común fue IV (34,6 %) y el nivel de Clark más frecuente fue IV (34,6 %). La ulceración estuvo en el 40,4 %. El subtipo nodular fue el de presentación más agresiva, donde el 100 % presentaron espesor de Breslow IV. Conclusiones. El subtipo de melanoma más común en nuestra población fue el de extensión superficial; el segundo en frecuencia fue el subtipo acral lentiginoso, que se localizó siempre en extremidades. Más del 50 % de los melanomas tenían espesor de Breslow mayor o igual a III, lo que impacta en el pronóstico.

Lycium humile Phil., is an extreme halophyte plant, endemic to Argentina, Bolivia, and Chile, which grows between 2000 and 4000 m a.s.l. in saline wetlands environments. The aim of this work is to identify morpho-anatomical and histochemical adaptive characteristics of the vegetative organs of L. humile linked to its tolerance to extreme environments of high altitude saline wetlands, in order to evaluate its potential as source of metabolites with commercial value or as source of resistance genes related to abiotic stress tolerance. To carry out the analysis, during the period of February 2018, samples were obtained from plants belonging to 4 populations of the species (5 individuals per population) located at the departments of Antofagasta de la Sierra, Santa María and Ambato, province of Catamarca, Argentina. Leaf and stems were studied using histochemical tests, conventional histological preparations and optical microscopy. Different foliar tissues were analyzed by scanning electron microscopy coupled to elemental X-ray diffraction spectroscopy (EDS). The leaves and stems of the analyzed populations showed uniform anatomical and histochemical features. It was stated for the first time that the succulence of the leaf is reached by the folding of the tissues evidenced in a tridimensional venation pattern. The presence of isolateral-radial mesophyll and aquifer parenchyma is highlighted as a unique feature characteristic for the species. The glandular trichomes and palisade parenchyma revealed complex contents of phenols, flavonoids, proteins, lipids, terpenes, and alkaloids; while the outer layer of the cuticles presented phenolic, terpenic and lipidic deposits. The EDS analysis showed crystalliferous idioblasts with high contents of Ca2+ and Na+. The stems exhibited hypodermis and air chambers formed in the cortical and medullary layers. These anatomical and histochemical characteristics of L. humile represent adaptive traits to saline high-altitude wetland environments. Its functionality in relation to the environment and its potential as source of resistance genes and metabolites interesting for the cosmetic and pharmaceutical industries are discussed. [ABSTRACT FROM AUTHOR]

Comparative neurobiology allows us to investigate relationships between phylogeny and the brain and understand the evolution of traits. Bats constitute an attractive group of mammalian species for comparative studies, given their large diversity in behavioural phenotypes, brain morphology, and array of specialised traits. Currently, the order Chiroptera contains over 1,450 species within 21 families and spans ca. 65 million years of evolution. To date, 194 Neotropical bat species (ca. 13% of the total number of species around the world) have been recorded in Central America. This study includes qualitative and quantitative macromorphological descriptions of the brains of 12 species from six families of Neotropical bats. These analyses, which include histological neuronal staining of two species from different families (Phyllostomus hastatus and Saccopteryx bilineata), show substantial diversity in brain macromorphology including brain shape and size, exposure of mesencephalic regions, and cortical and cerebellar fissure depth. Brain macromorphology can in part be explained by phylogeny as species within the same family are more similar to each other. However, macromorphology cannot be explained by evolutionary time alone as brain differences between some phyllostomid bats are larger than between species from the family Emballonuridae despite being of comparable diverging distances in the phylogenetic tree. This suggests that faster evolutionary changes in brain morphology occurred in phyllostomids — although a larger number of species needs to be studied to confirm this. Our results show the rich diversity in brain morphology that bats provide for comparative and evolutionary studies. La neurobiología comparativa nos permite investigar las relaciones entre filogenia y el cerebro, y comprender la evolución de rasgos. Los murciélagos constituyen un grupo atractivo de especies de mamíferos para estudios comparativos, dada su gran diversidad en fenotipos de comportamiento, morfología cerebral y variedad de rasgos especializados. Actualmente, el orden de murciélagos Chiroptera contiene más de 1,450 especies a lo largo de 21 familias y abarcando ca. 65 millones de años de evolución. 194 especies de murciélagos Neotropicales han sido registrados en América Central en la actualidad. Este estudio incluye descripciones macromorfológicas cualitativas y cuantitativas de cerebros de doce especies de seis familias de murciélagos Neotropicales. Estos análisis, que incluyen tinción neuronal usando una técnica histológica en dos especies de diferentes familias (Phyllostomus hastatus y Saccopteryx bilineata), muestran una diversidad sustancial en la macromorfología cerebral, incluida la forma y el tamaño del cerebro, la exposición de las regiones mesencefálicas y la profundidad de las fisuras corticales y cerebelosas. La macromorfología del cerebro puede explicarse en parte por la filogenia, ya que las especies dentro de la misma familia son más similares entre sí. Sin embargo, la macromorfología no puede explicarse solo por el tiempo evolutivo, ya que entre algunas especies de murciélagos filostómidos hay mayores diferencias que entre especies de la familia Emballonuridae, a pesar de tener distancias divergentes comparables en el árbol filogenético. Esto sugiere que se produjeron cambios evolutivos más rápidos en la morfología del cerebro en filostómidos, aunque para confirmar esto es necesario estudiar un mayor número de especies. Nuestros resultados muestran la gran diversidad en la morfología del cerebro que proporcionan los murciélagos para estudios comparativos y evolutivos. [ABSTRACT FROM AUTHOR]

Copyright of Spanish Journal of Palaeontology is the property of Socieadad Espanola de Paleontologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Zoologica Lilloana is the property of Fundacion Miguel Lillo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The mammary gland has its embryological origin, later it differentiates histologically and anatomically; over time new findings have been discovered, as well as new terminology. The aim of this work was to describe the update on the morphological aspects of the mammary gland. A search for articles was carried out in different primary and secondary sources of scientific literature such as: Pubmed, Embase, WOS, Scopus, Scielo, obtaining 623 publications, 53 selected for review and 17 included. The breast has been updated in morphological aspects; the ducts are located close to the areola and the nipple where they open directly, the branches of the ducts are located close to the areola; the nipples have between 15 and 20 lobes, of which only 5-9 are true mammary duct orifices, there are no lactiferous sinuses, so the milk flows directly into the nipple, in terms of innervation of the breast they are innervated by the anterior and lateral cutaneous branches of the intercostal nerves. The existing evidence demonstrates the need for permanent studies in the field of morphology, as described in this review article on the breast. In different aspects, such as terms, functions and structures. This information is important for undergraduate and postgraduate training, and its use in the clinic, identification of pathologies, lactation clinics, among others. [ABSTRACT FROM AUTHOR]

El estudio del tracto digestivo de especies de la familia Eleotridae ha sido poco abordado a pesar de su importancia para entender la fisiología de los peces, su nutrición, asociaciones tróficas y aplicación en acuicultura. El chame Dormitator latifrons es una de las especies de la familia Eleotridae con gran potencial para la acuicultura, de la cual se conoce muy poco sobre su sistema digestivo. Este trabajo describe la morfología e histología del tracto digestivo de D. latifrons y compara las variables morfométricas entre estadios y sexos, en 127 ejemplares recolectados en un sistema de cultivo. A cada pez se le registraron los datos biométricos y posteriormente se extrajo el tracto digestivo el cual fue descrito, medido por secciones, y se fijaron fragmentos de tejido en formalina neutra al 10 % para análisis histológico. Las muestras de tejido fueron procesadas por la técnica de inclusión en parafina y teñidas con hematoxilina y eosina; los datos morfométricos fueron comparados entre estadios y sexos. El tracto digestivo estuvo compuesto por boca, faringe, esófago, estómago, intestino y ano; y cada sección presentó las cuatro capas de tejido concéntrico (mucosa, submucosa, muscular y adventicia/serosa) características del sistema digestivo de los peces. Morfológicamente lo más distintivo fue la presencia de un estómago tubular en forma de “I”, bien delimitado y con abundantes glándulas gástricas tubulares. La longitud y el índice intestinales fueron significativamente diferentes entre estadios y sexos, posicionando a los juveniles en el nivel trófico omnívoro mientras que los adultos se posicionan como herbívoros; el análisis por sexo mostró un índice intestinal significativamente menor en las hembras respecto a los machos. Los resultados muestran un comportamiento politrófico del chame D. latifrons que podría estar asociado al hábitat, a la disponibilidad de alimento, o la madurez sexual. La presencia de un estómago funcional permite clasificar a esta especie como gástrica y abre la posibilidad de estudios futuros sobre la fisiología digestiva de esta especie y composición de la dieta idónea para organismos cultivados, temas hasta ahora muy poco atendidos.

The teaching of basic biomedical sciences at the Cienfuegos University of Medical Sciences had its beginnings in 1982 with the opening of the first year of the medical career at the Medical Sciences Faculty of that southern province, initially dependent, since the methodological point of view, from the Villa Clara Medical Sciences Higher Institute. The directors, professors and technicians who began teaching with the first Carlos J. Finlay Medical Sciences detachment are mentioned in this research, the result of the Commander in Chief Fidel Castro Ruz’ thought to universalize the teaching of medical sciences throughout the country. As the forty years of this teaching event in the Cienfuegos province were completed in 2022, this research serves as acknowledgement to the basic biomedical sciences teaching initiators in this province.

Se describen las fases carposporofíta y tetrasporofita de Gracilaria blodgettii a partir de estudios morfológicos e histológicos. se colectaron talos vegetativos y reproductivos entre Mayo y Octubre de 2006, y Septiembre a Diciembre de 2008, en la Bahía de Santa Catalina en isla providencia, Caribe Colombiano. El carposporofito presenta cistocarpos a lo largo del talo, con morfología oblonga, conformada por un núcleo y rodeado por un pericarpio interno medular estructurando el tejido nutritivo hacia el carposporofito. El tetrasporofito corresponde a individuos diploides cuyo hábito es similar al carposporofito, y su morfología se caracteriza por la presencia de tetrasporangios cruciados de forma ovada a cúbicos.

El estudio analiza las características de la piel en dos especies de anfibios, Leptodactylus mystacinus y Leptodactylus apepyta, que presentan áreas ecotonales en común. Para ello se tomaron muestras de la región dorsal, ventral e inguinal de ejemplares adultos de ambas especies, de cuatro especímenes, dos de cada especie, pertenecientes a la colección Herpetológica del Museo Nacional de Historia Natural del Paraguay (MNHNP) y se procesaron histológicamente. Los datos icrométricos de la piel se registraron y analizaron estadísticamente con un nivel de confianza de 95%, encontrándose que el promedio del grosor de la piel. Difiere entre las especies, en L. apepyta, el grosor promedio es de 128.44 µm en la región dorsal, 81.36 µm en la ventral y 74.41 µm en la inguinal, mientras que en L. mystacinus es de 139.81 µm, 49.31 µm y 102.92 µm en las mismas regiones, respectivamente. También se encontraron variaciones morfológicas en la epidermis y dermis de ambas especies, los análisis estadísticos revela una distribución normal de los datos, concluyéndose que, aunque los tegumentos de ambas especies son similares a los de otros anfibios, existen diferencias significativas en el grosor de la piel, siendo L. mystacinus más gruesa. Además, se observa la presencia de la capas de Eberth-Katschenko y glán- dulas con una diferenciación en su maduración para ambas especies.

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Aim: To describe the clinical manifestation and the diagnostic process of a patient with peritoneal tuberculosis as a clinical challenge in a tertiary referral center in a Latin American country. Case description: A 61-year-old male patient from the urban area of Cali consulted for edema in the lower limbs, ascites, hyporexia, weight loss, cachexia, thrombocytopenia, elevated transaminases, imaging of splenomegaly and pulmonary, hepatic, and peritoneum nodules. Initially, neoplasia was suspected, but the adenosine deaminase report and histopathology were consistent with the diagnosis of peritoneal tuberculosis. Conclusion: Peritoneal tuberculosis represents the sixth cause of extrapulmonary tuberculosis, after lymphatic, pleural, osteoarticular, genitourinary, and meningeal manifestations. Despite having surveillance systems, peritoneal tuberculosis is still considered the most challenging infectious disease to reach a definitive diagnosis. We present a case of disseminated tuberculosis in which invasion of the peritoneum was verified, for which tetraconjugate treatment was started; however, the patient showed an adverse hepatic reaction and died due to multisystem involvement of a pulmonary infectious complication. [ABSTRACT FROM AUTHOR]

The Iberá macrosystem has several types of vegetation, where in addition to aquatic and marsh vegetation are found hydrophilic forests, savannahs with a predominance of grasses and grasslands with shrub species interspersed among the herbaceous species. Previous studies of leaf anatomy were only carried out on aquatic and marsh plants. In this work, leaf anatomy was studied in predominant or frequent species growing in grasslands and forests of the Iberá macrosystem, with the aim of detecting leaf structural characters that may represent adaptive value to these environments. Leaves of 42 species corresponding to 26 families were analyzed with optical and scanning electron microscopy. Statistical analysis was performed based on a data matrix of the anatomical characters analyzed, also including the environment types in which the species studied grow. The analyzed characters of the epidermis were: cuticle design, stomata, trichomes, design of epidermal cells of both epidermis, where a predominance of identical morphology on both sides was observed. In the mesophyll, the type, presence and form of calcium and inulin crystals, and distribution of glandular structures such as laticifers, cavities and secretory cells were evaluated. The data reported are discussed in relation to the available literature. No constant characters associated with a particular vegetation type are found. Species living in the same environment do not have similar anatomical features, as shown by clustering analysis (UPGMA). [ABSTRACT FROM AUTHOR]

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Copyright of Revista Nacional de Odontología is the property of Universidad Cooperativa de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

La presente investigación evaluó el crecimiento de las papilas ruminales y espesor del rumen en terneros sometidos a dos raciones alimenticias distintas. Se establecieron dos grupos homogéneos de tratamiento, cada uno compuesto por cuatro individuos (T1 y T2). Los terneros del grupo T1 recibieron calostro, leche y alfalfa fresca, mientras que los terneros del grupo T2 recibieron la misma composición que el primer grupo, complementada con un alimento concentrado. El destete ocurrió a los 60 días de edad para ambos grupos. Después del destete, los terneros fueron alimentados y se recolectaron muestras de la pared del rumen de cuatro regiones anatómicas diferentes de cada ternero. Las dimensiones de las papilas ruminales se midieron macroscópicamente, y se llevó a cabo una histología utilizando el método de inclusión en parafina con tinción H&E. Los terneros del grupo T1 mostraron valores promedio de 1,38 ± 0,250 mm para la longitud de la papila, 3,63 ± 0,245 mm para el grosor de la pared del rumen, 1,77 ± 0,312 mm para la capa muscular y 0,5 ± 0,005 mm para la capa serosa. En cambio, los animales del grupo T2 alcanzaron una longitud promedio de papila de 3,13 ± 0,479 mm, con un grosor de pared de 7,13 ± 0,855 mm, un grosor de capa muscular de 3,00 ± 0,408 mm y un grosor de capa serosa de 1,02 ± 0,019 mm. En conclusión, la ración alimenticia suplementada con concentrado resultó en valores elevados de crecimiento de las papilas ruminales, junto con un notable desarrollo en la capa serosa, la capa muscular y en general en la pared del rumen en terneros.

To evaluate the histological adverse effects of alendronate administered systemically and topically in combination with orthodontic movement by intense force. Thirty-six 24-week-old female Wistar rats, ovariectomized, were used and divided into three groups (n = 12/group): control, locally treated with saline (0.07 ml/kg/week) (group 1) and experimental, treated with alendronic acid systemically (0.07 mg/kg/week) (group 2) and locally (7 mg/kg/week) (group 3). At 14 days, an orthodontic anchor was installed in the right first molar, and a force of 144 cN was applied for 28 days. The samples were processed for histological evaluation. Descriptive statistics, Shapiro-Wilk tests, one-way ANOVA with Bonferroni correction, one-way repeated measures ANOVA and chi-square tests were performed. All tests were statistically significant at p <0.05. The adverse events found in all groups were inflammation and osteoclastic activity. In the bisphosphonate-treated groups, there were statistically significant differences (p = 0.005) in the osteoclastic activity between the two hemiarcates. All rats in group 2 presented paralytic ileus. Compared to local administration, systemic treatment with alendronic acid produces more adverse effects, such as inflammation, fibrinoid necrosis, and osteoclastic activity. During the application of intense forces, it was not possible to show that there is necrosis associated with bisphosphonates. [ABSTRACT FROM AUTHOR]

Copyright of Acta Veterinaria Brasilica is the property of Acta Veterinaria Brasilica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The teaching of basic biomedical sciences at the Cienfuegos University of Medical Sciences had its beginnings in 1982 with the opening of the first year of the medical career at the Medical Sciences Faculty of that southern province, initially dependent, since the methodological point of view, from the Villa Clara Medical Sciences Higher Institute. The directors, professors and technicians who began teaching with the first Carlos J. Finlay Medical Sciences detachment are mentioned in this research, the result of the Commander in Chief Fidel Castro Ruz’ thought to universalize the teaching of medical sciences throughout the country. As the forty years of this teaching event in the Cienfuegos province were completed in 2022, this research serves as acknowledgement to the basic biomedical sciences teaching initiators in this province. [ABSTRACT FROM AUTHOR]