143 results on '"Hiroyuki Ohi"'
Search Results
2. Imaging in Propeller Flap Surgery
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Rei Ogawa, Hiroyuki Ohi, and Shimpei Ono
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Propeller ,Island Flaps ,Gold standard (test) ,030230 surgery ,Magnetic resonance angiography ,Computed tomographic angiography ,03 medical and health sciences ,Doppler sonography ,0302 clinical medicine ,Imaging Tool ,030220 oncology & carcinogenesis ,medicine ,Surgery ,In patient ,Radiology ,business - Abstract
Since propeller flaps are elevated as island flaps and most often nourished by a single perforator nearby the defect, it is challenging to change the flap design intraoperatively when a reliable perforator cannot be found where expected to exist. Thus, accurate preoperative mapping of perforators is essential in the safe planning of propeller flaps. Various methods have been reported so far: (1) handheld acoustic Doppler sonography (ADS), (2) color duplex sonography (CDS), (3) perforator computed tomographic angiography (P-CTA), and (4) magnetic resonance angiography (MRA). To facilitate the preoperative perforator assessment, P-CTA is currently considered as the gold standard imaging tool in revealing the three-dimensional anatomical details of perforators precisely. Nevertheless, ADS remains the most widely used tool due to its low cost, faster learning, and ease of use despite an undesirable number of false-positive results. CDS can provide hemodynamic characteristics of the perforator and is a valid and safer alternative particularly in patients in whom ionizing radiation and/or contrast exposure should be limited. Although MRA is less accurate in detecting smaller perforators of caliber less than 1.0 mm and the intramuscular course of perforators at the present time, MRA is expected to improve in the future due to the recent developments in technology, making it as accurate as P-CTA. Moreover, it provides the advantage of being radiation-free with fewer contrast reactions.
- Published
- 2020
3. Hand function and cost effectiveness after collagenase injection versus aponeurectomy for Dupuytren’s contracture: results of the CeCORD-J study
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Yuichi Hirase, Takeshi Oguchi, Katsuyuki Iwatsuki, Akinori Sakai, Naoto Tsubokawa, Yasunori Hattori, Hideo Yasunaga, Takaaki Shinohara, Shiro Urata, Michiro Yamamoto, Tetsuro Onishi, Rikuo Shinomiya, Hitoshi Hirata, Katsuhiro Tokutake, Toshikazu Tanaka, Hiroyuki Kato, Etsuhiro Nakao, Hiroyuki Ohi, Masanori Hayashi, Shohei Omokawa, Norimasa Iwasaki, Tsuyoshi Tajika, Hiroyuki Gotani, Ryosuke Kakinoki, Kunitaka Menuki, Ataru Igarashi, Toshikazu Kurahashi, Toru Sunagawa, Kazuhiro Otani, Yuichiro Matsui, Hiroshi Koshima, Masahiro Tatebe, Yoshiaki Yamanaka, Rhoji Kajiwara, Nobuyuki Okui, Takanobu Nishizuka, Yoshio Kaji, Eiichi Nagayoshi, Akimasa Morita, Hiroaki Sakano, Hideo Hasegawa, Yoshitaka Tanaka, Kentaro Watanabe, Yuri Kanno, Katsumi Tanaka, Masao Nishiwaki, Hiroyasu Ikegami, and Tetsu Katsumura
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medicine.medical_specialty ,Hand function ,Collagenase injection ,Cost effectiveness ,business.industry ,Aponeurectomy ,medicine ,Dupuytren's contracture ,business ,medicine.disease ,Surgery - Abstract
BackgroundThis study compared hand function and the cost effectiveness of treatment between collagenase Clostridium histolyticum (CCH) injection and limited fasciectomy for patients with Dupuytren’s contracture (DC).Materials and MethodsThe CeCORD-J study is a prospective, multicenter, non-randomized controlled, observational study of two parallel groups. Participants were DC patients with multiple affected fingers, including flexion contracture of the proximal interphalangeal (PIP) joint. The primary outcome was the Hand10 score, as a patient-reported outcome measure. We set secondary outcomes of EQ-5D-5L score, degree of extension deficit, and direct cost. Propensity score adjustment was used to balance differences in patient characteristics between groups.ResultsParticipants comprised 52 patients in the Collagenase group and 26 patients in the Surgery group. Hand10 score was significantly better in the Collagenase group at 1 and 2 weeks. EQ-5D-5L score was significantly higher in the Collagenase group at 8 weeks. Mean direct cost was 248,000 yen higher in the Surgery group than in the Collagenase group. Extension deficit angle of the PIP joint was significantly larger in the Collagenase group at 26 weeks.ConclusionsCCH injection provides better short-term hand function and cost effectiveness than surgery.UMIN-CTR (UMIN000029826)
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- 2021
4. Experimental Study for Understanding the Characteristics of a Floating Axis Wind Turbine under Wind and Wave Conditions
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Hidetaka Senga, Keitaro Kunishi, Gaku Fujita, Tomotake Imaoka, Hiroyuki Ohira, and Hiromichi Akimoto
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floating axis wind turbine ,model experiment ,mooring ,Technology - Abstract
Floating offshore wind turbines (FOWTs) are suitable for Japan’s coastal waters. As one of the unique concepts of FOWTs, the floating axis wind turbine (FAWT) is a type of vertical axis wind turbine (VAWT) that actively uses the features of VAWTs to specialize in an area of the floating type. In this study, FAWT behaviors under wind and wave conditions were experimentally investigated in a water tank to understand its dynamic characteristics. The experimental results reveal that although the floater of the FAWT is relatively small compared to its rotor size, its heave response is comparable to those of the other FOWTs using different floaters. Moreover, the FAWT shows a high stability under rated operation and during sudden changes in the wind.
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- 2024
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5. Efficacy and safety of novel collagen conduits filled with collagen filaments to treat patients with peripheral nerve injury: A multicenter, controlled, open-label clinical trial
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Masaomi Saeki, Hiroyuki Gotani, Kentaro Watanabe, Hideki Okamoto, Junya Imatani, Ryogo Nakamura, Toshiyasu Nakamura, Kenji Tanaka, Hiroyuki Ohi, and Hitoshi Hirata
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Adult ,Male ,0301 basic medicine ,medicine.medical_specialty ,Nerve guidance conduit ,Biocompatible Materials ,Wrist ,law.invention ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Electrical conduit ,Randomized controlled trial ,Peripheral Nerve Injuries ,law ,medicine ,Humans ,General Environmental Science ,Guided Tissue Regeneration ,business.industry ,Recovery of Function ,Middle Aged ,Nerve Regeneration ,Surgery ,Clinical trial ,Treatment Outcome ,surgical procedures, operative ,030104 developmental biology ,medicine.anatomical_structure ,Peripheral nerve injury ,General Earth and Planetary Sciences ,Female ,Collagen ,Open label ,business ,030217 neurology & neurosurgery ,Sensory nerve - Abstract
Introduction The safety and efficacy of using artificial collagen nerve conduits filled with collagen filaments to treat nerve defects has not been fully studied in humans. We conducted a multicenter, controlled, open-label study to compare the safety and efficacy of artificial nerve conduit grafts with those of autologous nerve grafts. Methods We included patients with a sensory nerve defect of ≤30 mm, at the level of the wrist or a more distal location, with the first-line surgical methods selected according to a patient’s preference. We compared sensory recovery using static two-point discrimination and adverse events between the artificial collagen nerve conduit and autologous nerve grafting. Results The artificial nerve conduit group included 49 patients, with a mean age of 42 years and nerve defect of 12.6 mm. The autologous nerve graft group included 7 patients, with historical data of an additional 31 patients, with a mean age of 36 years and nerve defect of 18.7 mm. The rate of recovery of sensory function at 12 months was 75% (36/49) for the artificial nerve conduit group and 73.7% (28/38) in the autologous nerve group. No serious adverse events directly associated with use of the artificial nerve conduit were identified. Conclusions The treatment of nerve defects ≤30 mm using artificial collagen nerve conduits was not inferior to treatment using autologous nerve grafts. Based on our data, the new artificial collagen nerve conduit can provide an alternative to autologous nerve for the treatment of peripheral nerve defects.
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- 2018
6. Outcomes of grafting intrasynovial tendons of the toes to the hands in 10 patients: a preliminary report
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Shigeharu Uchiyama, Masanori Hayashi, Hiroyuki Ohi, Hiroyuki Kato, T Kanda, and M Mukoda
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Adult ,Male ,musculoskeletal diseases ,medicine.medical_specialty ,Tendon grafting ,030230 surgery ,Thumb ,Tendons ,Young Adult ,03 medical and health sciences ,Active motion ,0302 clinical medicine ,Tendon Injuries ,Preliminary report ,Finger Injuries ,medicine ,Humans ,Complication rate ,Range of Motion, Articular ,Tendon graft ,Retrospective Studies ,030222 orthopedics ,Flexor tendon ,business.industry ,Middle Aged ,Toes ,Surgery ,body regions ,Treatment Outcome ,medicine.anatomical_structure ,Female ,Interphalangeal Joint ,business ,Joint Capsule - Abstract
Ten patients had intrasynovial tendon grafting harvested from the toes for secondary flexor tendon reconstruction in nine fingers and one thumb in our institutes from 2009 to 2014. These patients were followed for a mean of 15 (range: 8–36) months. The ranges of total active motion of the proximal and distal interphalangeal joints of these nine fingers were 143° (range: 108–175°) and of the metacarpophalangeal and interphalangeal joints of one thumb were 110°. In conclusion, this technique is feasible and gives a good result when successful but with a high complication rate.Level of Evidence IV
- Published
- 2017
7. Imaging Studies for Preoperative Planning of Perforator Flaps
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Shimpei Ono, Hiromitsu Hayashi, Rei Ogawa, and Hiroyuki Ohi
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medicine.medical_specialty ,Preoperative planning ,medicine.diagnostic_test ,business.industry ,Vascular anatomy ,Computed tomography ,030230 surgery ,Computed tomographic angiography ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,cardiovascular system ,Medicine ,Surgery ,cardiovascular diseases ,Radiology ,business ,Perforator flaps - Abstract
The vascular anatomy of perforators varies between individuals; thus, accurate preoperative assessment of perforators is essential for safely planning perforator flaps. Perforator computed tomographic angiography (P-CTA) with multidetector-row computed tomography (MDCT) is one of the best available methods to precisely reveal the 3-dimensional anatomic details of perforators. The aim of this report is to describe the authors' experience using P-CTA with MDCT for detecting the perforating vessel preoperatively and a step-by-step approach to harvest perforator flaps based on this technique. This report also provides a comprehensive review of literature on other preoperative assessment tools of perforators.
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- 2017
8. P1414: CRP IS A CONVENIENT PREDICTIVE MARKER OF THE ANTIPYRETIC EFFECT OF TOCILIZUMAB ON CRS DURING CAR-T CELL THERAPY
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Shota Yokoyama, Takahide Ara, Shinpei Harada, Yuto Mori, Yuta Hasegawa, Hiroyuki Ohigashi, Toshihiro Matsukawa, Atsushi Yasumoto, Souichi Shiratori, Masahiro Onozawa, Masao Nakagawa, Kaoru Kahata, Tomoyuki Endo, Daigo Hashimoto, Hideki Goto, and Takanori Teshima
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
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9. P1526: THE RISK OF DEVELOPING PNEUMOCYSTIS JIROVECII PNEUMONIA PERSISTS EVEN AFTER COMPLETION OF IMMUNOSUPPRESSANTS IN ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION RECIPIENTS
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Takahide Ara, Shota Yokoyama, Yuta Hasegawa, Hiroyuki Ohigashi, Toshihiro Matsukawa, Atushi Yasumoto, Souichi Shiratori, Hideki Goto, Masahiro Onozawa, Masao Nakagawa, Kaoru Kahata, Tomoyuki Endo, Daigo Hashimoto, and Takanori Teshima
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Diseases of the blood and blood-forming organs ,RC633-647.5 - Published
- 2023
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10. Microsurgical Flaps in Repair and Reconstruction of the Hand
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Shimpei Ono, Kevin C. Chung, Hiroyuki Ohi, and Sandeep J. Sebastin
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030222 orthopedics ,medicine.medical_specialty ,Microsurgery ,Hand function ,Ideal (set theory) ,Esthetics ,business.industry ,Soft tissue ,030230 surgery ,Plastic Surgery Procedures ,Hand ,Outcome (game theory) ,Surgical Flaps ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,Patient satisfaction ,Patient Satisfaction ,Medicine ,Humans ,Orthopedics and Sports Medicine ,business ,Perforator Flap ,Perforator flaps - Abstract
The authors' strategy for soft-tissue coverage of the hand is presented. The concept of replacing like with like and reconstruction with similar adjacent tissue enhances functional and aesthetic outcomes. In this viewpoint, the pedicle perforator flap is an ideal flap. A decision-making algorithm to select an ideal flap for a particular hand defect is challenging, requiring experiential consideration of functional outcome, appearance, donor-site morbidity, and patient satisfaction. To assist surgeons in determining the most appropriate flap with more evidence, studies are necessary to compare the outcomes of each flap by evaluating hand function, aesthetics, donor site morbidity, and patient satisfaction.
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- 2017
11. Imaging Studies for Preoperative Planning of Perforator Flaps: An Overview
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Shimpei, Ono, Hiromitsu, Hayashi, Hiroyuki, Ohi, and Rei, Ogawa
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Adult ,Male ,Angiography ,Humans ,Wounds and Injuries ,Tomography, X-Ray Computed ,Perforator Flap - Abstract
The vascular anatomy of perforators varies between individuals; thus, accurate preoperative assessment of perforators is essential for safely planning perforator flaps. Perforator computed tomographic angiography (P-CTA) with multidetector-row computed tomography (MDCT) is one of the best available methods to precisely reveal the 3-dimensional anatomic details of perforators. The aim of this report is to describe the authors' experience using P-CTA with MDCT for detecting the perforating vessel preoperatively and a step-by-step approach to harvest perforator flaps based on this technique. This report also provides a comprehensive review of literature on other preoperative assessment tools of perforators.
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- 2016
12. Determination of Severity of Murine IgA Nephropathy by Glomerular Complement Activation by Aberrantly Glycosylated IgA and Immune Complexes
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Yusuke Suzuki, Yuichi Tanaka, Isao Ohsawa, Jan Novak, Stacy Hall, Hitoshi Suzuki, Azusa Hashimoto, Hiroyuki Ohi, Rhubell Brown, and Yasuhiko Tomino
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Immunoglobulin A ,Chromatography, Gas ,Glycosylation ,Kidney Glomerulus ,chemical and pharmacologic phenomena ,Antigen-Antibody Complex ,urologic and male genital diseases ,Mannose-Binding Lectin ,Complement factor B ,Immunoglobulin G ,Pathology and Forensic Medicine ,Mice ,Immune system ,medicine ,Animals ,Complement Activation ,Mannan-binding lectin ,biology ,Monosaccharides ,Models, Immunological ,Reproducibility of Results ,Glomerulonephritis, IGA ,Regular Article ,Glomerulonephritis ,medicine.disease ,Complement system ,Immunology ,biology.protein ,Properdin ,Female ,Chromatography, Liquid - Abstract
The pathogenic roles of glomerular deposition of components of the complement cascade in IgA nephropathy (IgAN) are not completely clarified. To investigate the pathologic role of complement pathways in IgAN, two IgAN-prone mouse models were examined. Grouped ddY (gddY) mice showed significant high proteinuria, severe glomerular lesions, and extracellular matrix expansion compared with high serum IgA (HIGA) mice but with similar intensity of glomerular IgA deposition. Glomerular activation of the classical, lectin, and alternative pathways was demonstrated by significantly stronger staining for complement (C)3, C5b-9, C1q, C4, mannose-binding lectin (MBL)-A/C, MBL-associated serine protease-2, and factor B and properdin in gddY mice than in HIGA mice. Similarly, the serum levels of IgA-IgG2a/IgM and IgA–MBL-A/C immune complexes and polymeric IgA were significantly higher in gddY mice than in HIGA mice. Moreover, the serum levels of aberrantly glycosylated IgA characterized by the binding of Sambucus nigra bark lectin and Ricinus communis agglutinin I were significantly higher in gddY mice than in HIGA mice. This aberrancy in glycosylation was confirmed by monosaccharide compositional analysis of purified IgA using gas-liquid chromatography. This study is the first to demonstrate that aberrantly glycosylated IgA may influence the formation of macromolecular IgA including IgA-IgG immune complexes and subsequent complement activation, leading to full progression of IgAN.
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- 2012
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13. Reliability and Validity of the Japanese Version of the Michigan Hand Outcomes Questionnaire: A Comparison with the DASH and SF-36 Questionnaires
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Toshiyasu Nakamura, Yukio Abe, Hiroyuki Ohi, Yasukazu Katsumi, Katsunori Inagaki, and Takashi Oda
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Adult ,Male ,medicine.medical_specialty ,SF-36 ,Adolescent ,Intraclass correlation ,Population ,Michigan hand outcomes questionnaire ,03 medical and health sciences ,Disability Evaluation ,Young Adult ,0302 clinical medicine ,Cronbach's alpha ,Japan ,Surveys and Questionnaires ,Dash ,medicine ,Humans ,Translations ,030212 general & internal medicine ,education ,Reliability (statistics) ,Rank correlation ,Aged ,Aged, 80 and over ,030222 orthopedics ,education.field_of_study ,business.industry ,Reproducibility of Results ,General Medicine ,Middle Aged ,Hand ,Patient Outcome Assessment ,Physical therapy ,Female ,business - Abstract
Background: The Michigan Hand Outcomes Questionnaire (MHQ) has shown reliability, validity and responsiveness and has been used to assess surgical outcomes mainly in North America. We established a Japanese version of the MHQ and evaluated its reliability and validity compared with both the short-form 36 (SF-36) questionnaire and the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire in a Japanese-speaking population. Methods: The MHQ was cross-culturally adapted to a Japanese version according to guidelines. Sixty-eight patients with hand conditions were enrolled in this study and answered the MHQ, DASH questionnaire and SF-36 questionnaire. The MHQ was completed again with an interval of one or two weeks. Reproducibility and internal consistency were statistically assessed by the test-retest method and calculating Cronbach's alpha. Spearman's rank correlation was calculated to assess associations between the MHQ and the SF-36 questionnaire as well as the DASH questionnaire. Results: The intraclass correlation coefficients of MHQ subscales ranged from 0.68 to 0.93. Aesthetics subscale of the left hand showed the lowest intraclass correlation but still a good correlation. Cronbach's alpha values of the MHQ ranged from 0.81 to 0.96 for all subscales. The absolute values of Spearman's rank correlation coefficient between MHQ subscales and DASH function/symptoms scores ranged from 0.49 to 0.82. Spearman's correlation coefficients of the MHQ total score to subscales of the SF-36 questionnaire ranged from 0.42 to 0.68. The strongest correlations were found between work performance of the MHQ and rolephysical of the SF-36 questionnaire. Conclusions: The Japanese version of the MHQ has adequate instrument properties for assessing hand outcomes compared with the SF-36 questionnaire as well as the DASH questionnaire.
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- 2016
14. Extraglomerular C3 deposition and metabolic impacts in patients with IgA nephropathy
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Satoshi Horikoshi, Hiroyuki Ohi, Masaya Ishii, Hiroyuki Inoshita, Kisara Onda, Seiji Nagamachi, Hiyori Suzuki, Azusa Hashimoto, Isao Ohsawa, Yasuhiko Tomino, Nobuyuki Sato, Gaku Kusaba, and Mamiko Shimamoto
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Adult ,Male ,medicine.medical_specialty ,Urinary system ,Renal function ,Gastroenterology ,Group A ,Group B ,Nephropathy ,Immunoenzyme Techniques ,Risk Factors ,Internal medicine ,medicine ,Humans ,Transplantation ,medicine.diagnostic_test ,business.industry ,Glomerulonephritis, IGA ,Complement C3 ,Prognosis ,medicine.disease ,Glomerular Mesangium ,Surgery ,Blood pressure ,Nephrology ,Disease Progression ,Female ,Renal biopsy ,business ,Body mass index ,Biomarkers ,Follow-Up Studies ,Glomerular Filtration Rate - Abstract
Background. The aim of the present study was to explore the significance of extraglomerular (Bowman’s capsule and/or arteriole) C3 (ex-C3) deposits in IgA nephropathy (IgAN). Methods. One hundred and seventy patients with IgAN were divided into two groups: Group A (n = 79), patients who did not have ex-C3 deposits, and Group B (n = 91), patients who had ex-C3 deposits. Results. At the time of renal biopsy, Group B was characterized by a marked increase in diastolic blood pressure, total cholesterol, triglyceride and low-density lipoprotein-cholesterol compared with those of Group A. After 4 years, the estimated glomerular filtration rate (eGFR) in Group B was significantly worse than that of Group A. Upon examination by electron microscopy, the arteriolar dense deposits in Group B were found to occur in significantly higher amounts than in Group A. One hundred and thirty-four patients underwent a 3-year follow-up study after intervention and were re-divided by therapeutic factors as follows: ‘conventional therapy’, treatment with anti-hypertensive drugs and/or anti-platelet drugs, and ‘aggressive therapy’, additional treatment with either tonsillectomy or corticosteroid. Patients treated with conventional therapy in Group B had significantly higher body mass index and levels of C3 and CH50 compared with other Groups. Aggressive therapy was significantly effective in urinary protein reduction in both Group A and Group B. Except for the patients who received aggressive therapy in Group A, the levels of the eGFR gradually declined. Conclusions. It appears that IgAN patients who have ex-C3 deposits have worse clinical outcomes.
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- 2012
15. Pathological Scenario with the Mannose-Binding Lectin in Patients with IgA Nephropathy
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Masaya Ishii, Isao Ohsawa, Yasuhiko Tomino, and Hiroyuki Ohi
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lcsh:Biotechnology ,Health, Toxicology and Mutagenesis ,lcsh:Medicine ,chemical and pharmacologic phenomena ,Review Article ,Biology ,urologic and male genital diseases ,Nephropathy ,Pathogenesis ,lcsh:TP248.13-248.65 ,Genetics ,medicine ,Humans ,Molecular Biology ,Mannan-binding lectin ,lcsh:R ,Glomerulonephritis, IGA ,General Medicine ,bacterial infections and mycoses ,medicine.disease ,MBL deficiency ,Immune complex ,Complement system ,Mannose-Binding Lectins ,Lectin pathway ,Immunology ,Disease Progression ,Alternative complement pathway ,Molecular Medicine ,Biotechnology - Abstract
A deeper understanding of the mechanism of complement activation may help to elucidate the pathogenesis of IgA nephropathy (IgAN). Traditionally, the activation of an alternative pathway (AP) has been recognized as an enhancer mechanism of glomerular damage. This paper documents contemporary information concerning the possible pathological mechanisms of the lectin pathway (LP) in the circulation and in the glomerulus. The circulating initiator of LP activation is not fully understood. However, ligands for mannose-binding lectin (MBL) which are among the starter molecules of the LP are aberrant glycosylated molecules-containing immune complex. Recent reports have focused onN-glycans on secretory IgA as a candidate ligand. Mesangial deposits of MBL are seen in 25% of patients with IgAN. Mesangial deposits of MBL and C4 and/or C4 breakdown products are implicated as markers for disease progression of IgAN. On the other hand, patients with MBL deficiency tend to show better clinical presentation and lower levels of urinary protein and serum creatinine than MBL-sufficient patients. It is now recognized that involvement of AP and LP constitutes an additional mechanism for explaining the progression of IgAN.
- Published
- 2012
16. An analysis of functional activity via the three complement pathways during hemodialysis sessions: a new insight into the association between the lectin pathway and C5 activation
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Hiroyuki Inoshita, Kisara Onda, Yasuhiko Tomino, Satoshi Horikoshi, Hiroyuki Ohi, Isao Ohsawa, and Mariko Tamano
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Transplantation ,hemodialysis ,business.industry ,C3a desArg ,medicine.medical_treatment ,Original Contributions ,C3a desarg ,chemical and pharmacologic phenomena ,Original Articles ,Pharmacology ,lectin pathway ,Complement system ,Classical complement pathway ,Nephrology ,Lectin pathway ,Immunology ,Alternative complement pathway ,medicine ,Functional activity ,complement ,Hemodialysis ,business ,Complement membrane attack complex ,C5a desArg - Abstract
Background. We have recently demonstrated that hemodialysis (HD) patients have significantly higher levels of functional complement activity (FCA) in all three pathways, i.e. the classical pathway, alternative pathway and lectin pathway (LP), than in age-matched controls, though the role of FCA during HD still remains unknown. Methods. Serial plasma or serum samples were obtained from five patients during HD in order to investigate the kinetics of complement components. The levels of the C5b-9 complex, the FCA of the three pathways, a derivative of C3a (C3a desArg) and a derivative of C5a (C5a desArg) in the samples were analyzed. Results. The levels of the C5b-9 complex at 60 min were significantly increased when compared with those at 0 min. Functional activities for all three pathways showed different patterns so the same tendency between pathways was not observed. The levels of C3a desArg and C5a desArg at 60 min were markedly increased when compared with those at 0 min. A Spearman’s rho test showed a strong positive correlation between functional LP activity and C5a desArg. Conclusions. These findings lead to new insights into the FCA during HD and suggest that functional LP activity has an important role in C5 activation.
- Published
- 2012
17. Guideline for Hereditary Angioedema (HAE) 2010 by the Japanese Association for Complement Research - Secondary Publication
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Misako Matsumoto, Masaru Nonaka, Yuichi Endo, Teizo Fujita, Norimitsu Inoue, Masashi Mizuno, Tsukasa Seya, Hiroshi Tsukamoto, Takahiko Horiuchi, Michiyo Hatanaka, Tetsuro Yamamoto, Nobutaka Wakamiya, Misao Matsushita, Hidechika Okada, Isao Ohsawa, Noriko Okada, Miki Nakao, Taroh Kinoshita, and Hiroyuki Ohi
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lcsh:Immunologic diseases. Allergy ,medicine.medical_specialty ,Angioedema ,business.industry ,angioedema ,Angioedemas, Hereditary ,General Medicine ,Guideline ,medicine.disease ,Dermatology ,Complement (complexity) ,Japan ,Hereditary angioedema ,Immunology ,allergic inflammation ,medicine ,Humans ,Immunology and Allergy ,complement ,medicine.symptom ,lcsh:RC581-607 ,business ,guideline - Abstract
This guideline was provided by the Japanese Association for Complement Research targeting clinicians for making an accurate diagnosis of hereditary angioedema (HAE), and for prompt treatment of the HAE patient in Japan. This is a 2010 year version and will be updated according to any pertinent medical advancements.
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- 2012
18. Serum Concentration of Complement Components of the Lectin Pathway in Maintenance Hemodialysis Patients, and Relatively Higher Levels of L-Ficolin and MASP-2 in Mannose-Binding Lectin Deficiency
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Hiroyuki Inoshita, Satoshi Horikoshi, Misao Matsushita, Yasuhiko Tomino, Kisara Onda, Masaya Ishii, Isao Ohsawa, Michiro Wakabayashi, Gaku Kusaba, and Hiroyuki Ohi
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biology ,business.industry ,medicine.medical_treatment ,Case-control study ,Lectin ,chemical and pharmacologic phenomena ,Hematology ,bacterial infections and mycoses ,MBL deficiency ,medicine.disease ,Complement system ,Nephrology ,Lectin pathway ,Immunology ,medicine ,biology.protein ,Hemodialysis ,Risk factor ,business ,Mannan-binding lectin - Abstract
Mannose-binding lectin (MBL), L-ficolin and MBL associated serine protease-2 (MASP-2) are molecules involved in initiation of the lectin pathway (LP) in the complement system. Although MBL deficiency is observed in almost 10% of healthy people, studies of associations between MBL deficiency and end-stage renal disease (ESRD) remain rare. The objective of the present study is to clarify the significance of the LP in maintenance hemodialysis (HD) patients, especially in terms of MBL levels. Two hundred and forty-four HD patients who had been followed up for 74±84months and 199 healthy controls were included in this study. Measurements of serum concentrations of MBL, L-ficolin, and MASP-2 were performed. Low serum MBL levels (
- Published
- 2011
19. Immune Complex-Mediated Complement Activation in a Patient with IgG4-Related Tubulointerstitial Nephritis
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Satoshi Horikoshi, Yasuhiko Tomino, Nobuyuki Sato, Hiroyuki Ohi, Gaku Kusaba, Yukihiko Takeda, Misao Matsushita, Isao Ohsawa, Takashi Kobayashi, Masaya Ishii, and Seiji Nagamachi
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Microbiology (medical) ,Pathology ,medicine.medical_specialty ,Published: August, 2011 ,Immunology ,Complement ,law.invention ,Classical complement pathway ,Immune system ,law ,parasitic diseases ,medicine ,Tubulointerstitial nephritis ,Immunology and Allergy ,IgG4 ,Immune complex ,biology ,business.industry ,Lectin ,Tubulointerstitial Nephritis ,Complement system ,Classical pathway ,biology.protein ,Electron microscope ,Complement membrane attack complex ,business - Abstract
A 59-year-old man was diagnosed with IgG4-related tubulointerstitial nephritis. His symptoms as well as laboratory and imaging findings were improved after initiation of steroid therapy. Serologically, he showed hypocomplementemia (C3 23 mg/dl, C4
- Published
- 2011
20. LOCKING PALMAR PLATE FIXATION FOR DORSALLY DISPLACED FRACTURES OF THE DISTAL RADIUS: A PRELIMINARY REPORT
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Yuji Takahashi, Koji Moriya, Hiroyuki Ohi, and Hidehiko Saito
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Adult ,Male ,Wrist Joint ,musculoskeletal diseases ,medicine.medical_specialty ,Radiography ,Palmar Plate ,Wrist pain ,Displaced fractures ,Fracture Fixation, Internal ,Young Adult ,Fixation (surgical) ,Fracture fixation ,Bone plate ,medicine ,Humans ,Range of Motion, Articular ,Aged ,Retrospective Studies ,Aged, 80 and over ,Fracture Healing ,business.industry ,General Medicine ,Middle Aged ,Wrist Injuries ,musculoskeletal system ,Surgery ,body regions ,Treatment Outcome ,Female ,medicine.symptom ,Radius Fractures ,Range of motion ,business ,Bone Plates ,Follow-Up Studies - Abstract
We reviewed a series of 62 consecutive patients with dorsally displaced fractures of the distal radius, including 20 extra-articular and 42 intra-articular fractures. All patients were treated with palmar locking plate systems at our institution between 2002 and 2006. After a minimum follow-up time of 12 weeks, the fractures had healed with satisfactory radiographic and functional results. According to the demerit point system of Gartland and Werley, 35 patients were rated excellent, 26 good, and one fair. In the good and fair groups, the demerit points were almost all for ulnar wrist pain. Our results suggest that palmar locking plate systems enable early functional mobilization with good reproducible radiographic and clinical outcomes. Since nine out of 62 patients had residual ulnar wrist pain at the final follow-up evaluation, further investigation of the pathogenesis of ulnar wrist pain is necessary to obtain better functional outcomes.
- Published
- 2011
21. Metabolic impact on serum levels of complement component 3 in Japanese patients
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Hiroyuki Inoshita, Isao Ohsawa, Kisara Onda, Nobuyuki Sato, Gaku Kusaba, Masaya Ishii, Hiroyuki Ohi, Satoshi Mano, Yasuhiko Tomino, Tomohito Gohda, and Satoshi Horikoshi
- Subjects
Male ,Microbiology (medical) ,medicine.medical_specialty ,medicine.medical_treatment ,Clinical Biochemistry ,Comorbidity ,Hyperuricemia ,Hematocrit ,chemistry.chemical_compound ,Insulin resistance ,Japan ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Immunology and Allergy ,Outpatient clinic ,Prospective Studies ,Metabolic Syndrome ,Complement component 3 ,medicine.diagnostic_test ,business.industry ,Insulin ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,Original Articles ,Complement C3 ,Hematology ,Middle Aged ,medicine.disease ,Lipids ,Medical Laboratory Technology ,Cross-Sectional Studies ,Endocrinology ,Diabetes Mellitus, Type 2 ,chemistry ,Hypertension ,Uric acid ,Female ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,Insulin Resistance ,Metabolic syndrome ,business ,Biomarkers - Abstract
The aim of this study was to explore the association between the serum concentration of complement component 3 (C3) and a variety of metabolic parameters. The study involved 125 patients in our outpatient clinic. Anthropometric and clinical laboratory data were collected and statistical associations between the serum concentration of C3 and other parameters were evaluated in a cross‐sectional as well as a prospective manner. A group of male patients with metabolic syndrome (Mets, n=35) were characterized by marked increase in serum concentrations of C3, body mass index (BMI), waist circumference, hemoglobin (Hb) A1c, insulin resistance (HOMA‐IR), triglyceride, uric acid, urinary protein, and Hb. In a one‐way analysis of variance of all subjects, the serum concentration of C3 was significantly elevated as the number of items of complying with the Mets diagnostic criteria increased. In 60 of 125 patients who did not have diabetes and were given anti‐lipogenetic medication, the serum concentration of C3 showed significant positive associations with serum levels of CH50, insulin, HOMA‐IR, total cholesterol, hematocrit, LDL‐c, C4, Hb, triglyceride, BMI, and albumin. In a prospective follow‐up evaluation (n=35), there was a significant positive association between ΔC3 (the second concentration of serum C3 minus the first concentration of serum C3)and ΔHOMA‐IR (the second concentration of HOMA‐IR minus the first concentration of HOMA‐IR). In conclusion, in Japanese patients, there is evidence implicating C3 concentration as a marker of Mets coinciding with insulin resistance. J. Clin. Lab. Anal. 24:113–118, 2010. © 2010 Wiley‐Liss, Inc.
- Published
- 2010
22. Evidence of latent pathogenesis of Propionibacterium acnes infection in a patient with renal sarcoidosis
- Author
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Isao Ohsawa, Masaya Ishii, Hiroyuki Ohi, Yoshinobu Eishi, Hiroyuki Inoshita, Shinji Hagiwara, Satoshi Horikoshi, Masashi Aizawa, Gaku Kusaba, Yasuhiko Tomino, and Hiyori Suzuki
- Subjects
Transplantation ,Pathology ,medicine.medical_specialty ,Kidney ,medicine.diagnostic_test ,biology ,business.industry ,Glomerulonephritis ,urologic and male genital diseases ,medicine.disease ,biology.organism_classification ,Propionibacterium acnes ,medicine.anatomical_structure ,Membranous nephropathy ,Nephrology ,Immunology ,medicine ,Sarcoidosis ,Renal biopsy ,business ,Nephritis ,Nephrotic syndrome - Abstract
A 49-year-old man with pulmonary sarcoidosis was admitted to our hospital because of nephrotic syndrome. Renal biopsy revealed membranous nephropathy and granulomatous interstitial nephritis. DNA and cell membrane constituents of Propionibacterium acnes were detected in the patient’s glomeruli and tubular epithelial cells. Treatment with corticosteroids and an antimicrobial agent resulted in remission of the nephrotic syndrome. This is the second case in which components of P. acnes were found in renal tissue, suggesting latent pathogenesis of P. acnes infection in renal sarcoidosis.
- Published
- 2009
23. A novel measurement method for activation of the lectin complement pathway via both mannose-binding lectin (MBL) and L-ficolin
- Author
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Hiroyuki Ohi, Shunichi Koide, Gaku Kusaba, Isao Ohsawa, Munehiro Nakata, Hiroyuki Inoshita, Masaya Ishii, Kisara Onda, Satoshi Horikoshi, Yasuhiko Tomino, Min Jin Gi, and Misao Matsushita
- Subjects
Proteases ,Immunology ,Mannose ,Enzyme-Linked Immunosorbent Assay ,chemical and pharmacologic phenomena ,Biology ,Binding, Competitive ,Mannose-Binding Lectin ,Acetylglucosamine ,chemistry.chemical_compound ,C-type lectin ,Lectins ,Humans ,Immunology and Allergy ,Complement Activation ,Mannan-binding lectin ,Phosphatidylethanolamines ,Lectin ,Complement C4 ,Complement Pathway, Mannose-Binding Lectin ,bacterial infections and mycoses ,Molecular biology ,Complement system ,Biochemistry ,chemistry ,Lectin pathway ,biology.protein ,Ficolin - Abstract
Mannose-binding lectin (MBL), L-ficolin and H-ficolin are human serum lectins, all of which form complexes with MBL-associated serine proteases (MASP). The lectin-MASP complexes bind to the surface of microbes, leading to activation of the lectin pathway of complement. Enzyme-linked immunosorbent assays (ELISA) of the lectin pathway activity reported so far determined the activity via either MBL or L-ficolin, but an assay of activity via plural host defense lectins has not been established. To measure the lectin pathway activation mediated by plural lectins simultaneously, we developed an ELISA system in which N-acetylglucosamine-pentamer conjugated to dipalmitoylphosphatidylethanolamine (GN5-DPPE) was employed as a ligand for the lectins. In our ELISA system, both purified MBL and L-ficolin isolated from serum diluted in a buffer containing high ionic NaCl bound to GN5-DPPE and activated C4. Purified H-ficolin was not capable of binding to GN5-DPPE. MBL and L-ficolin in MBL-sufficient serum also bound to GN5-DPPE and activated C4. Mannose and N-acetylgalactosamine inhibited binding of MBL and L-ficolin to GN5-DPPE, respectively. MBL-deficient serum that had been depleted of L-ficolin did not exhibit C4 activation, but addition of both or either purified MBL and/or L-ficolin to the serum restored the activation in a dose-dependent manner. Thus, C4 cleaving activity could be evaluated with the co-existence of MBL and L-ficolin in vitro. In conclusion, we propose a novel method using GN5-DPPE for investigating the MBL- and L-ficolin-dependent lectin pathway and anticipate that this method will be useful in innate immunity and clinical research.
- Published
- 2009
24. Changes of serum IgA /C3 ratio in patients with IgA nephropathy
- Author
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Hiroyuki Ohi, Fumiko Kodama, Isao Ohsawa, Satoshi Horikoshi, Kenji Yamaji, Yuko Inami, and Yasuhiko Tomino
- Subjects
medicine.medical_specialty ,business.industry ,Internal medicine ,Medicine ,In patient ,Serum iga ,business ,medicine.disease ,Gastroenterology ,Nephropathy - Published
- 2008
25. Low CR1 (C3b Receptor) Level on Erythrocytes Is Associated with Poor Prognosis in Hemodialysis Patients
- Author
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Noriko Okada, Mariko Tamano, and Hiroyuki Ohi
- Subjects
Nephrology ,medicine.medical_specialty ,Erythrocytes ,Anemia ,medicine.medical_treatment ,Hepatitis C virus ,medicine.disease_cause ,Gastroenterology ,Predictive Value of Tests ,Renal Dialysis ,Risk Factors ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,Immune Complex Diseases ,Dialysis ,business.industry ,General Medicine ,Middle Aged ,Prognosis ,medicine.disease ,Red blood cell ,medicine.anatomical_structure ,Erythropoietin ,Immunology ,Receptors, Complement 3b ,Kidney Failure, Chronic ,Hemodialysis ,business ,Kidney disease ,medicine.drug - Abstract
Background: Erythropoietin in patients under dialysis treatment for renal failure is low which induces anemia. Treatment with recombinant erythropoietin (rEPO) has been used routinely as a supplement treatment for these patients. Immune complexes (IC) react with complement and bind to CR1 on erythrocytes (E-CR1), and are transported to the liver and/or spleen where IC removal and degradation occurs. The erythrocytes then return to circulation where they bind to additional IC. There are some patients whose E-CR1 expression is low with chronic anemia in spite of rEPO treatment. We hypothesized that in hemodialysis (HD) patients altered host defense against infection is associated with low levels of E-CR1. We examined if low E-CR1 in dialysis patients constitutes a risk factor for reduced host defense and poor outcome. Methods: In 95 HD patients, E-CR1 was quantified using a monoclonal E-CR1 antibody and FACS analysis followed by clinical course studies for 5 years. Results: The patients were divided into three groups by E-CR1 level. Percent survival for the low E-CR1 group (53.3%) was significantly lower than the high E-CR1 group (86.4%) (p < 0.01). There were more hepatitis C virus-positive patients within the low E-CR1 group (27.3%) than in the high E-CR1 group (4.7%) (p < 0.05). Furthermore, 10 patients with the lowest E-CR1 levels had severe complications, notably infection at an arteriovenous fistula. Conclusion: A reduced E-CR1 level might be a risk factor for reduced host defense and can be used as a predicting factor for poor prognosis in a HD patient.
- Published
- 2007
26. Multi-step lymphomagenesis deduced from DNA changes in thymic lymphomas and atrophic thymuses at various times after γ-irradiation
- Author
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Yukio Mishima, Kenya Kamimura, Hiroyuki Ohi, Masaki Maruyama, Ryo Kominami, and K Sasai
- Subjects
Cancer Research ,Lymphoma ,BCL11B ,Population ,Receptors, Antigen, T-Cell ,Loss of Heterozygosity ,Mice, SCID ,Thymus Gland ,medicine.disease_cause ,Polymerase Chain Reaction ,Loss of heterozygosity ,Mice ,Genetics ,medicine ,Animals ,PTEN ,education ,Molecular Biology ,DNA Primers ,Mice, Inbred BALB C ,education.field_of_study ,Base Sequence ,biology ,DNA, Neoplasm ,Thymus Neoplasms ,medicine.disease ,medicine.anatomical_structure ,biology.protein ,Cancer research ,Bone marrow ,Trisomy ,Carcinogenesis ,Whole-Body Irradiation ,Signal Transduction - Abstract
Whole-body gamma-irradiation to mice causes thymic atrophy where a population of precancerous cells with mutation can be found. Thus, clonal growth and DNA changes at Bcl11b, Ikaros, Pten, Notch1 and Myc were examined in not only thymic lymphomas but also in atrophic thymuses at various times after irradiation. Clonal expansion was detected from the distinct patterns of rearrangements at the TCRbeta receptor locus in a fraction of atrophic thymuses at as early as 30 days after irradiation. This expansion may be in part owing to the rearranged TCRbeta signaling because the transfer of bone marrow cells with the rearrangement and the wild-type locus into severe-combined immunodeficiency mice showed preferential growth of the rearranged thymocytes in atrophic thymus. Loss of heterozygosity (LOH) at Bcl11b and trisomy of Myc were found at high frequencies in both lymphomas and atrophic thymuses, and in contrast, LOH at Ikaros and Pten were rare in atrophic thymuses but prevalent in lymphomas. Notch1 activation was detected in lymphomas and in atrophic thymuses only at a late stage. Similar patterns of DNA changes were found in atrophic thymuses induced in Bcl11b(+/-) mice. These results suggest the order of genetic changes during lymphomagenesis, Bcl11b and Myc being at the early stage; whereas Ikaros, Pten and Notch1 at the late stage.
- Published
- 2007
27. Hypercomplementemia in adult patients with IgA nephropathy
- Author
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Kisara Onda, Isao Ohsawa, Hiroyuki Ohi, Satoshi Horikoshi, Yasuhiko Tomino, Teizo Fujita, Mariko Tamano, and Michiro Wakabayashi
- Subjects
Adult ,Male ,Microbiology (medical) ,Adolescent ,Clinical Biochemistry ,Enzyme-Linked Immunosorbent Assay ,Complement factor I ,Complement C1 Inactivator Proteins ,urologic and male genital diseases ,Complement Hemolytic Activity Assay ,Complement factor B ,Nephropathy ,C1-inhibitor ,medicine ,Humans ,Immunology and Allergy ,Serpins ,Aged ,biology ,Complement C4b-Binding Protein ,Biochemistry (medical) ,Public Health, Environmental and Occupational Health ,Complement C5 ,Fibrinogen ,Glomerulonephritis, IGA ,Glomerulonephritis ,Complement System Proteins ,Original Articles ,Hematology ,Middle Aged ,Prognosis ,medicine.disease ,Immunoglobulin A ,Complement system ,Proteinuria ,Medical Laboratory Technology ,Complement Factor H ,Immunology ,biology.protein ,Properdin ,Female ,Complement C1 Inhibitor Protein ,Complement Factor B - Abstract
IgA nephropathy (IgAN) is the most common form of chronic glomerulonephritis. Although glomerular deposition of complement components is well known, the evidence of serological complement activation in IgAN is inconclusive. We hypothesized that serum levels of complement components and regulatory proteins in patients with IgAN are correlated with its pathogenesis. In the present study we measured complement components in 50 patients with IgAN and 50 healthy volunteers. C5, C1 inhibitor, factor B, C4 binding protein, factor H, and factor I were measured with the use of single radial immunodiffusion. Mannose‐binding lectin (MBL) and properdin (P) were measured by enzyme‐linked immunosorbent assay (ELISA). The correlations among complements in the sera of patients with clinical gradings for IgAN (i.e., the good prognosis group, relatively good prognosis group, relatively poor prognosis group, and poor prognosis group) were evaluated. CH50, C4, factor B, P, factor I, and factor H were significantly higher in IgAN patients than in healthy controls. There were significant correlations between C5 and C4 binding protein, between C3 and C5, or between C4 and factor B in patients with IgAN. In the poor prognosis group, C4 binding protein was significantly higher than in the other groups of IgAN patients. hypercomplementemia occurs in IgAN and is associated with an increase in complement regulatory protein (CRP). C4 binding protein analyses can be used to predict disease prognosis. J. Clin. Lab. Anal. 21:77–84, 2007. © 2007 Wiley‐Liss, Inc.
- Published
- 2007
28. Serum levels of complement regulatory proteins in patients with IgA nephropathy
- Author
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Kisara Onda, Hiroyuki Ohi, Isao Ohsawa, Mariko Tamano, and Yasuhiko Tomino
- Subjects
business.industry ,Immunology ,medicine ,In patient ,medicine.disease ,business ,Complement (complexity) ,Complement system ,Nephropathy - Published
- 2007
29. Hepatic arterial infusion chemotherapy using fluorouracil, epirubicin, and mitomycin C for patients with liver metastases from gastric cancer after treatment failure of systemic S-1 plus cisplatin
- Author
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Hiroshi Seki, Hiroshi Yabusaki, Toshirou Ozaki, and Hiroyuki Ohi
- Subjects
Adult ,Male ,medicine.medical_specialty ,Mitomycin ,Gastroenterology ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Catheters, Indwelling ,Hepatic Artery ,Stomach Neoplasms ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Infusions, Intra-Arterial ,Radiology, Nuclear Medicine and imaging ,Treatment Failure ,Aged ,Epirubicin ,Tegafur ,Cisplatin ,Aged, 80 and over ,Radiological and Ultrasound Technology ,business.industry ,Standard treatment ,Mitomycin C ,Liver Neoplasms ,Cancer ,Combination chemotherapy ,General Medicine ,Middle Aged ,medicine.disease ,Surgery ,Survival Rate ,Drug Combinations ,Oxonic Acid ,Treatment Outcome ,Fluorouracil ,030220 oncology & carcinogenesis ,Disease Progression ,Female ,business ,Tomography, X-Ray Computed ,After treatment ,medicine.drug - Abstract
Background For patients with liver metastases from gastric cancer (LMGC), combination chemotherapy with fluoropyrimidines and platinum agents has been recognized as standard treatment. However, the prognosis of hepatic progression after first-line treatment failure remains poor. When hepatic progression occurs, hepatic arterial infusion (HAI) chemotherapy may be helpful for preventing disease progression. Purpose To retrospectively assess the feasibility and efficacy of HAI chemotherapy using 5-fluorouracil, epirubicin, and mitomycin C (FEM) for patients with LMGC after failure of systemic S-1 plus cisplatin. Material and Methods We reviewed the records of patients who received HAI chemotherapy using FEM for LMGC that progressed during systemic S-1 plus cisplatin treatment while extrahepatic disease was decreased or did not appear. HAI chemotherapy was given as second-line therapy using 5-fluorouracil (330 mg/m2 weekly), epirubicin (30 or 40 mg/m2 every 4 weeks), and mitomycin C (2.7 mg/m2 biweekly). Results Fourteen patients were analyzed. Toxicity of HAI chemotherapy was generally mild. The objective response rate was 42.9%, including a complete response rate of 14.3%. Median times to hepatic and extrahepatic progression were 9.2 and 7.4 months, respectively. Of 12 patients with documented progression after HAI chemotherapy, 10 patients (83.3%) received additional treatment, including irinotecan or taxanes. Overall, median survival was 12.7 months. Conclusion Our findings suggest that HAI chemotherapy using FEM is a feasible and effective treatment for patients with LMGC after failure of systemic S-1 plus cisplatin. HAI chemotherapy employed in the second-line setting is useful for achieving long-term disease control of LMGC.
- Published
- 2015
30. Acquired Loss of Erythrocyte Complement Receptor Type 1 in Patients with Diabetic Nephropathy Undergoing Hemodialysis
- Author
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Michiro Wakabayashi, Kisara Onda, Hiroyuki Ohi, Teizo Fujita, Mariko Tamano, and Yasuhiko Tomino
- Subjects
Adult ,Male ,medicine.medical_specialty ,Erythrocytes ,Physiology ,medicine.medical_treatment ,CD59 Antigens ,Deoxyribonuclease HindIII ,CD59 ,Ligands ,Diabetic nephropathy ,Complement Receptor Type 1 ,Renal Dialysis ,Diabetes mellitus ,Internal medicine ,Genetics ,medicine ,Humans ,Diabetic Nephropathies ,Decay-accelerating factor ,Aged ,CD55 Antigens ,business.industry ,General Medicine ,Middle Aged ,Complement deficiency ,medicine.disease ,Introns ,Receptors, Complement ,Complement system ,Endocrinology ,Nephrology ,Immunology ,Female ,Hemodialysis ,business ,human activities ,Polymorphism, Restriction Fragment Length - Abstract
Background: Complement receptor type 1 on erythrocytes (E-CR1) plays important roles not only in the regulation of complement activation, but also the clearance of immune complexes. Reduced E-CR1 was previously found in patients undergoing hemodialysis (HD). We investigated whether the E-CR1level in HD patients with diabetic nephropathy (DMN) is decreased. The levels of decay accelerating factor (DAF) and CD59 on erythrocytes (E) were also determined to ascertain whether the loss of CR1 is a specific phenomenon or other complement regulatory proteins are also affected. Methods: The levels of CR1, DAF, and CD59 on E were analyzed in 176 HD patients with DMN, 101 HD patients with non-diabetes mellitus renal diseases (non-DMN), and 108 healthy individuals. Hind III restriction fragment length polymorphism of intron 27 of the CR1 gene was analyzed. The serum-soluble CR1 levelwas measured by ELISA. Results: The E-CR1 level was significantly lower in the DMN group than the non-DMN group (p < 0.0001) and healthy individuals (p < 0.05). The E-CR1 level was significantly higher in the non-DMN group than in healthy individuals (p < 0.01). The levels of E-DAF and E-CD59 were significantly lower in the DMN group than non-DMN group (DAF, p < 0.01; CD59, p < 0.0001). Within each genotype of the CR1 gene, the E-CR1 level was significantly lower in the DMN group than in the non-DMN group and healthy individuals (non-DMN, p < 0.01; healthy individuals, p < 0.05). The serum-soluble CR1 level was significantly higher in the DMN group than non-DMN group and control group (p < 0.01 each). However, soluble CR1 did not correlate with E-CR1. Conclusion: Acquired loss of E-CR1 was found among HD patients with DMN. From the viewpoint of host defense, it may be a prognostic factor.
- Published
- 2006
31. Localization of pulmonary surfactant protein D in kidney
- Author
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Machiko Ikegami, Hiroyuki Ohi, Satoshi Mano, Jeffrey A. Whitsett, Yasuhiko Tomino, and Mariko Tamano
- Subjects
Kidney ,medicine.anatomical_structure ,Chemistry ,medicine ,Pulmonary surfactant protein D ,Molecular biology - Published
- 2006
32. Serum Mannose-Binding Lectin Levels in Maintenance Hemodialysis Patients: Impact on All-Cause Mortality
- Author
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Morito Endo, Takayuki Fujita, Isao Ohsawa, Yoshinobu Fuke, Hiroyuki Ohi, Sukemasa Sudo, Misao Matsushita, Teizo Fujita, Atsushi Satomura, and Koichi Matsumoto
- Subjects
Adult ,Male ,Nephrology ,medicine.medical_specialty ,medicine.medical_treatment ,chemical and pharmacologic phenomena ,Mannose-Binding Lectin ,Renal Dialysis ,Cause of Death ,Internal medicine ,medicine ,Humans ,Prospective Studies ,Survival rate ,Aged ,Cause of death ,Mannan-binding lectin ,Aged, 80 and over ,biology ,business.industry ,C-reactive protein ,Acute-phase protein ,Lectin ,General Medicine ,Middle Aged ,Prognosis ,bacterial infections and mycoses ,Survival Rate ,Immunology ,biology.protein ,Kidney Failure, Chronic ,Female ,Hemodialysis ,business ,Biomarkers - Abstract
Background/Aims: Mannose-binding lectin (MBL) is characteristic of an acute-phase-reacting protein like C-reactive protein (CRP). However, the prognostic value of the serum MBL level has not been examined. The aim of this study was to evaluate whether the serum MBL level can predict all-cause mortality in hemodialysis (HD) patients. Methods: A total of 131 patients without active infection, who had been on maintenance HD for at least 2 years, were included in this study. The serum MBL, high-sensitivity CRP (hs-CRP) level, nutrition markers, and biochemical parameters were measured in June 1999. The cohort was then followed prospectively for 36 months, and clinical data were recorded. Results: The MBL level of the 131 HD patients was 9.054 ± 5.115 µg/ml (mean ± SD). During the follow-up period, 18 patients (9 males and 9 females) died and 113 (64 males and 49 females) survived. The two leading causes of death were cardiovascular events (n = 6, 33.3%) and infection (n = 4, 22.2%). The serum MBL level was significantly lower among the nonsurvivors (6.596 ± 4.990 µg/ml) than among the survivors (9.445 ± 5.046 µg/ml; p < 0.05). There was a significant, although very weak, correlation between the MBL level and albumin level (p < 0.05), but there was no correlationbetween the MBL level and the hs-CRP level. The patients were divided into two groups according to the serum MBL level (5 µg/ml). Multivariate analysis of factors predicting all-cause mortality in multivariate logistic regression analysis identified a serum MBL level Conclusions: Our findings suggest that the serum MBL level is a significant predictor of outcome in HD patients. HD patients with a low level of serum MBL should be carefully monitored.
- Published
- 2005
33. A case of renal sarcoidosis with complement activation via the lectin pathway
- Author
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Kunimi Maeda, Yasuhiko Tomino, Mitsumine Fukui, Shinji Hagiwara, Satoshi Horikoshi, Yoshinobu Eishi, Yuichiro Makita, Fumiko Kodama, Kyoichi Tashiro, Yusuke Suzuki, and Hiroyuki Ohi
- Subjects
DNA, Bacterial ,Pathology ,medicine.medical_specialty ,Sarcoidosis ,Glomerulonephritis, Membranoproliferative ,Glomerular Mesangial Cell ,Interstitial nephritis ,Anti-Inflammatory Agents ,Complement Membrane Attack Complex ,urologic and male genital diseases ,Mannose-Binding Lectin ,Methylprednisolone ,Complement C4b ,Humans ,Medicine ,Propionibacterium acnes ,Complement Activation ,Lung ,Gram-Positive Bacterial Infections ,Mannan-binding lectin ,Heparin ,business.industry ,Anticoagulants ,Glomerulonephritis ,Complement C3 ,Middle Aged ,medicine.disease ,Peptide Fragments ,Glomerular Mesangium ,Histiocytosis, Langerhans-Cell ,Nephrology ,Lectin pathway ,Immunology ,Nephritis, Interstitial ,Prednisone ,Mesangial proliferative glomerulonephritis ,Drug Therapy, Combination ,Female ,Warfarin ,Lung Diseases, Interstitial ,business ,Complement membrane attack complex ,Nephritis - Abstract
A 57-year-old woman with pulmonary sarcoidosis was admitted to the hospital because of an elevation of serum creatinine and blood urea nitrogen. On admission, the laboratory data suggested interstitial nephritis without proteinuria and hematuria, whereas a renal biopsy showed granulomatous interstitial nephritis and mild mesangial proliferative glomerulonephritis. Immunoglobulin and C1q deposits were negative, but mannose-binding lectin, C3, C4d, and C5b-9 deposits were marked in the glomerular mesangial areas. The lectin pathway of complement activation may have contributed to the development of glomerular injury in this patient. DNA of Propionibacterium acnes , which is now strongly suspected as the pathogen of sarcoidosis, was detected in the patient's glomerular mesangial cells; tubular epithelial cells, which were involved in granulomatous inflammation; and mononuclear cells in epithelioid granulomas by in situ hybridization. These findings may add new insights to the pathogenesis of renal sarcoidosis, including its relation to infection, because mannose-binding lectin plays a crucial role in the host defense against various pathogens. From this case of renal sarcoidosis, it is hypothesized that P acnes may be involved in pathogenesis of granulomatous interstitial nephritis and that it plays a role in glomerular complement activation via the lectin pathway.
- Published
- 2005
34. Decrease of erythrocyte complement receptor type 1 (E-CR1) in patients undergoing hemodialysis with diabetic nephropathy
- Author
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Kisara Onda, Mariko Tamano, Hiroyuki Ohi, Satoshi Horikoshi, Kunimi Maeda, Michiro Wakabayashi, and Yasuhiko Tomino
- Subjects
Diabetic nephropathy ,medicine.medical_specialty ,Complement Receptor Type 1 ,business.industry ,Internal medicine ,medicine.medical_treatment ,medicine ,In patient ,Hemodialysis ,medicine.disease ,business ,Gastroenterology - Published
- 2005
35. Contents Vol. 97, 2004
- Author
-
Heidrun Zuckermann-Becker, Hans-H. Neumayer, Christoph Melzer, Isao Ohsawa, Megan Griffith, Olimpia Ortega, Thomas Cairns, Gitana Voss, Ching-Shiang Chi, Morito Endo, Michael Haase, R.J. D’Souza, Cornelia Scholle, A Carreño, Luz Lozano, Mutsuko Hidaka, Dietmar Krausch, Simone Rosseau, Paloma Gallar, Stanislao Morgera, Mar Muñoz, Yoshinobu Fuke, A. J. Nicholls, Simon C. Satchell, Lin-Shien Fu, Chao-Yan Shien, Adam McLean, Andrew J. Palmer, Aniana Oliet, Hiroyuki Ohi, M. Beaman, Mariko Tamano, Milagros Ortiz, Ortrud Vargas-Hein, Takayuki Fujita, Alvaro Molina, Isabel Rodríguez, Tomasz Stompór, Ajay Dhaygude, David Taube, and Ana Vigil
- Subjects
Traditional medicine ,Nephrology ,business.industry ,Medicine ,General Medicine ,business - Published
- 2004
36. Quantitative polymorphism of complement receptor type 1 (CR1) in patients undergoing haemodialysis
- Author
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Hiroyuki Ohi, Mariko Tamano, Yasuhiko Tomino, and Sukemasa Sudo
- Subjects
medicine.medical_specialty ,Proteolysis ,medicine.medical_treatment ,Complement receptor ,Dialysis tubing ,Complement Receptor Type 1 ,Renal Dialysis ,Internal medicine ,Endopeptidases ,medicine ,Humans ,Allele ,Aged ,Transplantation ,Polymorphism, Genetic ,medicine.diagnostic_test ,business.industry ,Complement C5 ,Membranes, Artificial ,Complement C3 ,medicine.disease ,Trypsin ,Receptors, Complement ,Endocrinology ,Nephrology ,Immunology ,Hemodialysis ,business ,Kidney disease ,medicine.drug - Abstract
Background. The level of complement receptor type 1 (CR1) on erythrocytes (E-CR1) is determined by the presence of high (H) or low (L) expression alleles. We investigated whether acquired loss of E-CR1 occurs in haemodialysis patients and, if so, which factors may contribute to acquired loss of E-CR1 in these patients. Methods. The E-CR1 level was determined in 195 Japanese haemodialysis patients, and we selected patients with a high or low E-CR1 level. In patients with low E-CR1 expression, sequence analysis of polymorphic sites (A3650G and C5507G) in the CR1 gene was performed. To assess the effect of the type of dialysis membrane used in the patients with low E-CR1 expression, the dialysis membrane was changed from a cellulose membrane to a biocompatible membrane (to a polyacrylonitrile membrane and then to a polysulfone membrane). To evaluate the susceptibility of E-CR1 to proteolysis, erythrocytes were incubated with various concentrations of trypsin, and the level of remaining CR1 on the erythrocytes was determined. Results. Among patients with high E-CR1 expression (n ¼ 30), 87% had HH alleles and 13% had HL alleles. Among patients with low E-CR1 expression (n ¼ 29), 24% had LL alleles, 45% had HL alleles and 31% had HH alleles. Nucleotides 3650G and 5507G in the CR1 gene were associated with the L allele. Nucleotides 3650A and 5507C were associated with the H allele. Only one patient with HH alleles had nucleotides 3650G and 5507C. Three months after changing the haemodialysis membrane, the E-CR1 level significantly increased (P
- Published
- 2004
37. Worsening Fluid Retention in a Patient with Hereditary Angioedema and End-stage Renal Disease
- Author
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Isao Ohsawa, Mutsuko Hidaka, Atsushi Satomura, Hiroyuki Ohi, Takayuki Fujita, Morito Endo, and Yoshinobu Fuke
- Subjects
Pediatrics ,medicine.medical_specialty ,Nephrotic Syndrome ,medicine.medical_treatment ,Complement C1 Inactivator Proteins ,urologic and male genital diseases ,End stage renal disease ,C1-inhibitor ,immune system diseases ,Immunopathology ,Internal Medicine ,Edema ,Humans ,Medicine ,cardiovascular diseases ,Angioedema ,Enzyme Inhibitors ,skin and connective tissue diseases ,Serpins ,biology ,business.industry ,food and beverages ,General Medicine ,Middle Aged ,medicine.disease ,Surgery ,Hereditary angioedema ,biology.protein ,Kidney Failure, Chronic ,Female ,Hemodialysis ,medicine.symptom ,business ,Complement C1 Inhibitor Protein ,Nephrotic syndrome ,Kidney disease - Abstract
A 60-year-old woman who was diagnosed with hereditary angioedema (HAE) developed nephrotic syndrome, with end-stage renal disease (ESRD) occurring about 2.5 years later. During her slide toward ESRD, she experienced three severe episodes of angioedema that each resulted in significant fluid retention. Though the therapeutic administration of C1-inhibitor concentrate was effective in controlling her angioedema, seemed ineffective in preventing her from developing ESRD requiring hemodialysis treatment. We theorized that the patient's low colloid osmolality and glomerular perfusion were important facilitators of her attacks of angioedema.
- Published
- 2004
38. The significance of PTH(1-84)/Large C-PTH fragments(=CAP/CIP) in the managements of bone metabolism of maintenance hemodialysis patients
- Author
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Katsuhiko Furuta, Toshiharu Maruyama, Atsushi Satomura, Hiroaki Hamada, Isao Ohsawa, Yoshinobu Fuke, Sukemasa Sudo, Toru Nibuya, and Hiroyuki Ohi
- Subjects
medicine.medical_specialty ,Endocrinology ,business.industry ,Internal medicine ,medicine ,Maintenance hemodialysis ,business ,Bone remodeling - Abstract
近年生物学的活性を有するPTH(1-84)=CAP (cyclase activatin PTH) を測定するwhole PTH assayが開発された. 従来区別できなかったPTH(7-84) 等のlarge C-PTH fragments=CIP (cyclase inhibitory PTH) がCAPに対して拮抗作用を有することが注目され, CAP/CIPの変化が無形性骨症の要因として検討されている. 今回ビタミンD経口投与中の維持血液透析患者群においてCAP/CIPが骨回転の指標となりうるか検討してみた. 透析歴4年以上で糖尿病歴, ビタミンDパルス歴のない90名を対象とし1997年1月から2001年3月までの間, 血清Ca, P濃度を毎週, iPTH, β2MGを2か月毎, 右第2中手骨骨密度をDIP法で6か月毎に測定した. さらに2001年4月total PTH=iPTH, CAP, CIP=iPTH-CAP, BAP, BGP, 1CTP等を測定し, iPTHにより低下群 (0-65pg/mL) 25名, 正常群 (65-200pg/mL) 43名, 亢進群 (200-pg/mL) 22名の3群に分け, 骨代謝マーカーとCAP/CIPの関係などを検討した. 透析歴は正常群で有意に短く, 1日あたりのalfacalcidol平均投与量および平均Ca×P積は亢進群で有意に増加していた. iPTHの平均は157.6±152.2pg/mLであり, 低下群でCAP/CIPは1.53±1.63と正常群+亢進群の3.32±2.47に比べて有意に低値を示した. BAP, BGP, 1CTPおよびBGP/1CTPの各骨代謝マーカーも低下群は低値を示し, このビタミンD投与中の患者群でもCAP/CIPが低値を示すことは低回転骨を示唆する可能性が示された. CAP/CIPはPTH分泌低下の指標と考えられたが, さらに骨組織との間に介在する他の要因の検討が必要と思われる.
- Published
- 2003
39. Recombinant EPO therapy increases erythrocyte expression of complement regulatory proteins
- Author
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Noriko Okada, Hiroyuki Ohi, Mariko Tamano, and Sukemasa Sudo
- Subjects
medicine.medical_specialty ,Erythrocytes ,Anemia ,Complement receptor 1 ,CD59 Antigens ,chemical and pharmacologic phenomena ,CD59 ,Glomerulonephritis ,Renal Dialysis ,Internal medicine ,Diabetes Mellitus ,medicine ,Humans ,Renal Insufficiency ,Erythropoietin ,Decay-accelerating factor ,Kidney ,CD55 Antigens ,biology ,business.industry ,Complement C3 ,Middle Aged ,medicine.disease ,Recombinant Proteins ,Receptors, Complement ,Complement system ,Endocrinology ,medicine.anatomical_structure ,Nephrology ,Chronic Disease ,biology.gene ,business ,medicine.drug ,Kidney disease - Abstract
One of the complications of hemodialysis (HD) therapy is anemia caused by erythropoietin (EPO) deficiency. Recombinant EPO (rEPO) has been used routinely as a supplemental treatment. Erythrocyte expression of the complement regulatory proteins decay accelerating factor (DAF) and CD59 restricts complement activation and inhibits hemolysis. We hypothesized that the efficacy of rEPO treatment may be caused in part by the ability of rEPO to increase erythrocyte expression of DAF and CD59.DAF, CD59, and complement receptor 1 (CR1) levels were analyzed for a group of 95 HD patients and compared with those of a control group. To evaluate effects of discontinuation of rEPO therapy, rEPO therapy was stopped for 12 HD patients until hematocrits decreased to less than 25%. DAF and CD59 levels then were reanalyzed.In the 95 HD patients, three factors correlated significantly: DAF and CD59 (r = 0.642), DAF and CR1 (r = 0.503), and CD59 and CR1 (r = 0.324), whereas no correlations were found in the group of 42 healthy controls. In the experiment in which rEPO therapy was discontinued, 8 of 12 patients reached the defined level of anemia 4 to 7 weeks after rEPO treatment had been withheld. Both DAF and CD59 levels decreased significantly after discontinuation of rEPO therapy (P0.01). DAF and CD59 levels increased in all 8 patients after rEPO treatment was reinitiated (P0.01), and CR1 levels increased in 5 of 8 patients. Four of 12 patients showed no evidence of anemia after discontinuation of rEPO treatment. In these patients, DAF, CD59, and CR1 levels did not change before or after withholding rEPO therapy.One of the mechanisms mediating the efficacy of EPO therapy is increased DAF and CD59 expression.
- Published
- 2003
40. Nephrotic Syndrome with Portal, Splenic and Renal Vein Thrombosis
- Author
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Yoshinobu Fuke, Morito Endo, Hiroyuki Ohi, Yuki Etoh, Kazunari Kumasaka, Isao Ohsawa, and Takayuki Fujita
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medicine.medical_specialty ,business.industry ,Renal vein thrombosis ,Glomerulonephritis ,medicine.disease ,Thrombosis ,Surgery ,Portal vein thrombosis ,Venous thrombosis ,Splenic vein ,cardiovascular system ,medicine ,Renal vein ,business ,Nephrotic syndrome - Abstract
Background: Thromboembolism is known as a major complication of nephrotic syndrome, but only 4 cases of portal vein thrombosis have been reported as a complication of nephrotic syndrome. All of these 4 cases had acute symptoms, and 3 of 4 were in relapsing phase of nephrotic syndrome when thrombi were found. We describe here a case of 51-year-old woman with fresh nephrotic syndrome that was asymptomatically complicated by portal, splenic and renal vein thrombosis. Conclusion: In the presence of fresh nephrotic syndrome of minimal change, asymptomatic and widely distributed, including portal vein, thrombus formation occurred. If the clinical course shows resistance to therapy, we must consider the complication of venous thrombosis. Anticoagulant therapy with heparin and warfarin was effective and all thrombi disappeared without any other complications.
- Published
- 2002
41. Subject Index Vol. 91, 2002
- Author
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Hiroyuki Sasamura, Olaf Hergesell, K. Tabei, Osamu Hotta, Norio Sunagawa, Ioannis Tzanakis, Emanuela Rizzioli, Mingcai Qiu, Paul F. Laflam, Gian Marco Ghiggeri, Yasufumi Kyuden, D. Ljubanovic, M.J. Nubé, W.E.M. Schouten, Carla Carasi, Joachim Lundahl, Mariko Miyazaki, Isao Ishikawa, Yasuhiko Ueda, Kazuhiko Funabiki, Michael Nomikos, Tatsuo Kobayashi, Maria Magalhães, Manabu Asano, Takayuki Fujita, Shaul M. Shasha, Revital Shurtz-Swirski, Susana Sampaio, H. Furuya, Kefaia El-Sayed Mohamed, Mitsuhiro Satoh, A. Bihorac, Ç. Özener, Renza Cristofani, Ikue Mori-Kudo, U. Assogba, Satoshi Ogata, A.J. van Houte, Shifra Sela, Hidetake Kurihara, Maria Rita Metelli, Kayoko Nomura, Giovanni Montini, Atsushi Satomura, S. Krizanac, Rodo O. von Vigier, Morito Endo, Jürgen Floege, Lara Alonso da Silva, Yasuharu Nomura, Satoko Honda, Manuel Pestana, Hassan Abd-El-Hady Ahmed, Omar da Rosa Santos, P. Cacoub, Toshihiro Shinosaki, Ikuko Miyai, Y. Asano, Yoshiharu Nishitani, Yoshio Taguma, Chien-Liang Chen, Chizuru Ishiguro, P. Xavier, Gianluca Caridi, Marina Martic, Jean-Pierre Guignard, Maura Zanolari Calderari, Akira Saito, Hua-Chang Fang, F. A. W. Kemperman, Huiqi Qu, Atsushi Yamauchi, Hisaya Tada, Gavin J. Becker, Koji Harada, Mohamed Abel-Kader Sobh, Galal Mohamed Amer, Luisa Murer, P A Conz, Hiroyuki Ohi, Ali Moshfegh, Armando A. Mendes, Osama Gheith, Daisuke Suzuki, Fatma Elhusseini, Ciro Tetta, Eugênio Pacelle Queiroz Madeira, Yoshinobu Fuke, Takashi Uzu, Kang-Ju Chou, Savvas Kazoulis, Manuel Palacín, Ioannis Christoulakis, P. Hausfater, G. Kantarcı, Hideyuki Kurioka, Ivano Moschèn, Stefan H. Jacobson, Graziella Zacchello, Satoshi Horikoshi, Atsushi Tsuchida, Ryoko Shimizu-Hirota, Petros Hatzilias, Alberto Bettinelli, Tim D. Hewitson, Raymond T. Krediet, J.C. Piette, Jacek Borawski, S. Tokay, Takafumi Ito, Mutsuo Taiji, Ronit Geron, Makoto Watanabe, M. Schoorl, Carlo Catalano, Pia Thylén, Péter Tóth-Heyn, Ching-Bun Chen, Rajiv Kumar, Shigemi Chiba, Stefan Bröer, Takao Saruta, Mahmoud El-Baz, Yuji Fujita, E. Akoğlu, Roberto Palla, Michał Myśliwiec, Toshiki Inokuchi, Yasuhiko Tomino, Yasuhiro Akai, E. Kusano, Yukiteru Asakimori, Maria Norpoth, Shih-Yuan Hung, Yee-Hsuan Chiou, Kristen J. Kelynack, Hideo Shiiki, Alberto A.E. Bertelli, Mari Kuroda, Yoshihiko Taniguchi, Kazumasa Hamano, Massimiliano Migliori, Steven McTaggart, P. Lebon, Toshio Miyata, Vincenzo Panichi, Gunilla Halldén, Soichi Haraguchi, Mario G. Bianchetti, Po-Tsang Lee, M.P.C. Grooteman, Hideaki Nakaya, Ken Takahara, Fabio Fabbian, Yutaka Yaguchi, Florian Lang, Anna Maria Bianchi, Birgit Kallinowski, Mitchell L. Halperin, M. Inoue, Mizuo Mifune, Masayuki Iwano, Masafumi Yamato, Stefano De Pietro, Hiroshi Noguchi, Koji Kuboki, Hsiao-Min Chung, Hideto Sakai, Tomokazu Nagano, Iwan Setiawan, Surinder Cheema-Dhadli, Nobuoki Kohno, Shan Lin, M. Vrkljan, Isao Shirato, Noriaki Yorioka, Adriana MacIntyre Innocenzi, Marcella Normanno, José Gerardo Oliveira, Matsuhiko Hayashi, Y. Ando, Lambertus Arisz, Krystyna Pawlak, Galina Shapiro, Ikuo Horigome, Takashi Furuta, Y. Akai, Batya Kristal, João Ramos, Mahmoud Wageh Rasem, Alexandra Albers, Sarantos Xirakis, Sérgio Fernando Ferreira Santos, Takao Kawamura, Martin Zeier, Hiroo Noshiro, Manuela Della Vella, Daniele Taccola, Isao Ohsawa, K. Galesic, Mie Ko, Sana Sayed Gazarin, Eduardo H. Garin, Guilherme Santoro-Lopes, Mutsuko Hidaka, Luca Giovannini, and Giulietta Sbragia
- Subjects
Index (economics) ,business.industry ,Statistics ,Medicine ,Subject (documents) ,business - Published
- 2002
42. Contents Vol. 91, 2002
- Author
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Steven McTaggart, Hisaya Tada, D. Ljubanovic, Gunilla Halldén, M.J. Nubé, Marina Martic, Yasufumi Kyuden, Luisa Murer, Vincenzo Panichi, W.E.M. Schouten, Eugênio Pacelle Queiroz Madeira, Mariko Miyazaki, Yasuhiko Ueda, Hidetake Kurihara, Maria Norpoth, Guilherme Santoro-Lopes, Mutsuko Hidaka, Galal Mohamed Amer, Ali Moshfegh, Carlo Catalano, Chien-Liang Chen, Stefano De Pietro, Koji Kuboki, Ryoko Shimizu-Hirota, P. Hausfater, Susana Sampaio, Yoshinobu Fuke, Manuel Palacín, Luca Giovannini, Savvas Kazoulis, Satoshi Ogata, A.J. van Houte, Ikuo Horigome, Graziella Zacchello, Alberto Bettinelli, Batya Kristal, Tim D. Hewitson, Isao Shirato, Noriaki Yorioka, Makoto Watanabe, Renza Cristofani, Sérgio Fernando Ferreira Santos, Takao Kawamura, Y. Akai, S. Krizanac, Jacek Borawski, Maura Zanolari Calderari, Nobuoki Kohno, Marcella Normanno, Giulietta Sbragia, Iwan Setiawan, U. Assogba, Mitsuhiro Satoh, Mutsuo Taiji, Y. Asano, Mahmoud El-Baz, Daisuke Suzuki, Gavin J. Becker, Akira Saito, Giovanni Montini, Stefan Bröer, Yuji Fujita, Revital Shurtz-Swirski, Paul F. Laflam, P A Conz, Ching-Bun Chen, Ioannis Christoulakis, Toshihiro Shinosaki, Takafumi Ito, Takashi Uzu, Yasuhiro Akai, Kang-Ju Chou, G. Kantarcı, Shan Lin, Atsushi Tsuchida, Hideo Shiiki, Mari Kuroda, Atsushi Satomura, Huiqi Qu, Soichi Haraguchi, Michał Myśliwiec, Masayuki Iwano, Masafumi Yamato, M. Vrkljan, Petros Hatzilias, Yoshihiko Taniguchi, Atsushi Yamauchi, Emanuela Rizzioli, Mingcai Qiu, Mohamed Abel-Kader Sobh, Sana Sayed Gazarin, Shigemi Chiba, M. Schoorl, E. Kusano, Yukiteru Asakimori, Anna Maria Bianchi, Birgit Kallinowski, Mario G. Bianchetti, Armando A. Mendes, Yutaka Yaguchi, Isao Ishikawa, Manuel Pestana, Pia Thylén, M. Inoue, Shifra Sela, Po-Tsang Lee, Massimiliano Migliori, Jean-Pierre Guignard, Olaf Hergesell, P. Lebon, Hideto Sakai, Yee-Hsuan Chiou, Kristen J. Kelynack, Alberto A.E. Bertelli, Osamu Hotta, Shaul M. Shasha, Tomokazu Nagano, Lambertus Arisz, Martin Zeier, H. Furuya, Kefaia El-Sayed Mohamed, A. Bihorac, Ç. Özener, Lara Alonso da Silva, Hiroshi Noguchi, Toshio Miyata, Takao Saruta, E. Akoğlu, Stefan H. Jacobson, Krystyna Pawlak, Ivano Moschèn, Maria Rita Metelli, Hsiao-Min Chung, Takashi Furuta, Adriana MacIntyre Innocenzi, Yoshiharu Nishitani, Kazumasa Hamano, Manabu Asano, Mizuo Mifune, Kayoko Nomura, Tatsuo Kobayashi, Gian Marco Ghiggeri, João Ramos, Mahmoud Wageh Rasem, José Gerardo Oliveira, Hassan Abd-El-Hady Ahmed, Hiroyuki Ohi, S. Tokay, Matsuhiko Hayashi, Osama Gheith, Carla Carasi, P. Xavier, Ikue Mori-Kudo, Hiroo Noshiro, Kazuhiko Funabiki, Michael Nomikos, Chizuru Ishiguro, Ikuko Miyai, Maria Magalhães, Péter Tóth-Heyn, Satoko Honda, Manuela Della Vella, Daniele Taccola, Gianluca Caridi, Yasuharu Nomura, Hideyuki Kurioka, Joachim Lundahl, P. Cacoub, Y. Ando, Ronit Geron, Toshiki Inokuchi, Eduardo H. Garin, Galina Shapiro, Roberto Palla, Shih-Yuan Hung, Yasuhiko Tomino, Ciro Tetta, Surinder Cheema-Dhadli, Alexandra Albers, Sarantos Xirakis, Florian Lang, Mitchell L. Halperin, Hideaki Nakaya, Ken Takahara, Fabio Fabbian, Jürgen Floege, Yoshio Taguma, Koji Harada, Hua-Chang Fang, F. A. W. Kemperman, Satoshi Horikoshi, Rodo O. von Vigier, Morito Endo, Omar da Rosa Santos, Norio Sunagawa, M.P.C. Grooteman, Fatma Elhusseini, Takayuki Fujita, Raymond T. Krediet, J.C. Piette, Rajiv Kumar, Hiroyuki Sasamura, K. Tabei, Ioannis Tzanakis, K. Galesic, Mie Ko, and Isao Ohsawa
- Subjects
Traditional medicine ,business.industry ,Medicine ,business - Published
- 2002
43. 急性腹症を呈した腸管浮腫の一例
- Author
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ISAO OHSAWA, NOZOMI MUGITANI, HIYORI SUZUKI, SEIJI NAGAMACHI, GAKU KUSABA, MASAYA ISHII, NOBUYUKI SATO, HIROYUKI OHI, and YASUHIKO TOMINO
- Published
- 2011
44. Cryoprecipitate of Patients with Cryoglobulinemic Glomerulonephritis Contains Molecules of the Lectin Complement Pathway
- Author
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Takayuki Fujita, Isao Ohsawa, Mutsuko Hidaka, Hiroyuki Ohi, Misao Matsushita, Teizo Fujita, Atsushi Satomura, Mariko Tamano, Morito Endo, and Yoshinobu Fuke
- Subjects
Adult ,Male ,Adolescent ,Immunology ,chemical and pharmacologic phenomena ,Complement factor I ,Biology ,Kidney ,Immune complex formation ,Glomerulonephritis ,Lectins ,medicine ,Chemical Precipitation ,Humans ,Immunology and Allergy ,Aged ,Mannan-binding lectin ,Serine Endopeptidases ,Complement System Proteins ,Middle Aged ,medicine.disease ,Hepatitis C ,Cryoglobulinemia ,Collectins ,Immune complex ,Complement system ,Mannose-Binding Protein-Associated Serine Proteases ,Lectin pathway ,Female ,Carrier Proteins - Abstract
Serological and histological studies were carried out to explore the role of the lectin complement pathway in the pathogenesis of cryoglobulinemic glomerulonephritis. Sixteen patients with mixed cryoglobulinemia type II with glomerulonephritis (GN) were enrolled. All cases had hepatitis C virus (HCV) infection. The serum concentration of mannose-binding lectin (MBL) was significantly higher in the GN patients than in the normal controls according to ELISA (P < 0.01). IgG, IgM, C1q, C4d, HCV envelope antigen, MBL, and MBL-associated serine protease-1 (MASP-1) could be visualized in the cryoprecipitate of the 16 patients by Dot blot assay. Renal biopsy specimens obtained from 3 patients were examined by immunohistochemistry, and the glomeruli strongly stained for IgG, IgM, MBL, MASP-1, C4d, C3c, and C3d in a fringe-like pattern. The pattern of HCV constituent deposition was partially fringe-like. The complement profiles of the 16 cases were distinctive; briefly, the serum levels of C1q, C2, and C3 were reduced, although the levels of circulating regulatory proteins (C1-inhibitor, factor H, and factor I) were in the normal range. The serum C4 level was significantly reduced. These results indicate that immune complex formation involves molecules of the lectin pathway and leads to organ damage in cryoglobulinemic glomerulonephritis.
- Published
- 2001
45. One-stage elongation of the third or fourth brachymetacarpia through the palmar approach
- Author
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Masahiro Koizumi, Hiroyuki Ohi, Hidehiko Saito, and Yuji Takahashi
- Subjects
External fixator ,Surgical approach ,business.industry ,One stage ,Anatomy ,Hand ,Radiography ,medicine.anatomical_structure ,medicine ,Humans ,Upper limb ,Dorsal approach ,Orthopedic Procedures ,Orthopedics and Sports Medicine ,Surgery ,Brachymetacarpia ,Metacarpus ,business - Abstract
The technique of 1-stage elongation of a short third or fourth metacarpal through the palmar approach was devised to avoid the drawbacks of the conventional procedures through the dorsal approach with or without using an external fixator. This technique was used in the treatment of 3 cases and the results and the technique are described.
- Published
- 2001
46. Decreased expression of signaling lymphocytic-activation molecule-associated protein (SAP) transcripts in T cells from patients with rheumatoid arthritis
- Author
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Takashi Horie, Shigemasa Sawada, Shigeyoshi Fujiwara, Ko Mitamura, Masami Takei, Hiroyuki Ohi, Tetsuyoshi Ishiwata, Tatsunari Nishi, Takahiro Ookubo, Katsutoshi Sasaki, Tetsuro Kuga, and Junnosuke Ryu
- Subjects
DNA, Complementary ,T-Lymphocytes ,Lymphocyte ,Molecular Sequence Data ,Immunology ,Immunoglobulins ,Receptors, Cell Surface ,Biology ,Virus ,Arthritis, Rheumatoid ,Immune system ,Signaling lymphocytic activation molecule ,Signaling Lymphocytic Activation Molecule Family Member 1 ,Antigens, CD ,medicine ,Humans ,Immunology and Allergy ,Cytotoxic T cell ,RNA, Messenger ,Glycoproteins ,Gene Expression Profiling ,X-linked lymphoproliferative disease ,General Medicine ,Middle Aged ,medicine.disease ,medicine.anatomical_structure ,Rheumatoid arthritis ,Mutation ,RNA ,Receptors, Virus ,Signal transduction - Abstract
The function of Epstein-Barr virus (EBV)-specific cytotoxic T cells is disturbed in rheumatoid arthritis (RA) patients but the mechanism for this disturbance has remained unknown. In a recent study searching for the causative gene of X-linked lymphoproliferative syndrome, the gene possibly linked to EBV-specific cytotoxic T cells or NK cell-mediated cytotoxic activity to EBV-infected cells was discovered, and its product is now referred to as signaling lymphocytic-activation molecule-associated protein (SAP) or Src homology 2 domain-containing protein (SH2D1A). In the present study, we attempted to investigate the involvement of the SAP gene in RA using a quantitative real-time PCR; the expression level of SAP transcripts in peripheral leukocytes or T cells was examined for patients with RA. The expression level of SAP transcripts in peripheral leukocytes of 21 RA patients was significantly lower than that of 13 normal individuals (P = 0.0007), four patients with palindromic RA, 11 with inactive systemic lupus erythematosus (SLE) or 17 with chronic renal diseases. The decreased expression of SAP transcripts in RA patients was also observed in peripheral CD2(+) T cells compared with normal individuals. There was no mutation in the coding region of SAP cDNAs derived from peripheral leukocytes of five RA patients. The decreased expression of SAP transcripts in peripheral leukocytes or T cells of RA patients might lead to the failure of the immune system to eliminate the EBV-infected synovial lining cells in joints of RA patients. Our findings have suggested that decreased expression of the SAP gene might be involved in the onset or progress of RA.
- Published
- 2001
47. Acute Alteration in Bone Mineral Density and Biochemical Markers for Bone Metabolism in Nephrotic Patients Receiving High-Dose Glucocorticoid and One-Cycle Etidronate Therapy
- Author
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Teizo Fujita, Atsushi Satomura, Isao Ohsawa, Mutsuko Hidaka, Morito Endo, and Hiroyuki Ohi
- Subjects
Adult ,Male ,medicine.medical_specialty ,Deoxypyridinoline ,Nephrotic Syndrome ,Time Factors ,Adolescent ,Bone density ,Prednisolone ,Endocrinology, Diabetes and Metabolism ,Osteocalcin ,Osteoporosis ,Etidronate Disodium ,Bone and Bones ,Bone resorption ,Bone remodeling ,chemistry.chemical_compound ,Endocrinology ,Bone Density ,Internal medicine ,medicine ,Humans ,Orthopedics and Sports Medicine ,Amino Acids ,Child ,Glucocorticoids ,Bone mineral ,Dose-Response Relationship, Drug ,business.industry ,Etidronic Acid ,Phosphorus ,Middle Aged ,Etidronic acid ,Alkaline Phosphatase ,medicine.disease ,chemistry ,Calcium ,Drug Therapy, Combination ,Female ,business ,Biomarkers ,medicine.drug - Abstract
It is widely known that glucocorticoids induce and accelerate osteoporosis. High-dose glucocorticoids are administrated daily to patients in the acute phase of nephrotic syndrome. It could be inferred that high-dose glucocorticoids rapidly decrease patients' basal bone mineral density (BMD) and this accelerates the natural progress of osteoporosis associated with aging or menopause. Nine nephrotic patients (male/female: 5/4) without previous prednisolone administration were chosen to measure BMD and the level of the markers for bone turnover before and after treatment for 3 months (total prednisolone administration: 4.5 +/- 0.0 g). Twenty-three patients under remission with prednisolone administration (male/female: 14/9) were included in the long-term treatment group. Patients in this group whose %YAM in the lateral lumbar spine was less than 89% were classified into a low BMD group (n = 10, male/female: 3/7). They were administered etidronate disodium at 200 mg/day for 14 days. BMD and % of young adult mean (YAM) in the lumbar spine (L2-L4 in lateral objection) and other regions were measured by dual-energy X-ray absorptiometry. As markers of bone metabolism, the urinary level of deoxypyridinoline (Dpd) was determined to evaluate osteogenesis, and serum osteocalcin was measured to evaluate bone resorption. BMD of the lumbar spine significantly decreased in the 3-month treatment group (752 +/- 96 mg/cm(2), 7 +/- 4% reduction) compared with the pretreatment group (810 +/- 85 mg/cm(2)). BMD in the long-term treatment group decreased continuously (683 +/- 135 mg/cm(2)). No significant differences were noted in other measurement sites. BMD in the etidronate treatment group increased significantly (597 +/- 55 mg/cm(2)) compared with the pretreatment group (549 +/- 76 mg/cm(2)). Etidronate did not change BMD at the sites with a normal BMD. Among the biochemical markers (BM) examined, the urinary level of Dpd (nMol/liter. Cr) significantly increased in the 3-month treatment group (8.6 +/- 5.1 nMol/liter.Cr) compared with the pretreatment group (5.8 +/- 2.0 nMol/liter. Cr). No significant differences were seen in the BMs measured in the long-term treatment group. The urinary Dpd level of the etidronate treatment group decreased (3.9 +/- 1.4 nMol/liter. Cr) compared with the pretreatment group. These data indicate that etidronate could improve the accelerated bone resorption. In conclusion, high-dose glucocorticoid therapy causes rapid bone resorption and accelerates the natural progress of osteoporosis associated with aging or menopause. Etidronate administration prevents the progress of osteoporosis in nephrotic patients. Preventive treatment should be performed when the estimated BMD in 3 months falls below the baseline by more than 7 +/- 4%, reaching the therapeutic range.
- Published
- 2000
48. Complement activation through the lectin pathway in patients with Henoch-Schönlein purpura nephritis
- Author
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Takayuki Fujita, Hiroyuki Ohi, Morito Endo, Isao Ohsawa, Teizo Fujita, and Misao Matsushita
- Subjects
Adult ,Male ,Immunoglobulin A ,Henoch-Schonlein purpura ,Adolescent ,IgA Vasculitis ,Glomerulonephritis, Membranoproliferative ,Kidney Glomerulus ,chemical and pharmacologic phenomena ,Nephropathy ,Lectins ,Humans ,Medicine ,Child ,Complement Activation ,Nephritis ,biology ,business.industry ,Glomerulonephritis ,medicine.disease ,Complement system ,Nephrology ,Lectin pathway ,Immunology ,biology.protein ,Mesangial proliferative glomerulonephritis ,Female ,business ,Mannose - Abstract
Henoch-Schönlein purpura nephritis (HSPN) is considered a form of systemic vasculitis of the small blood vessels with immune pathogenesis. In this disorder, the complement system is recognized as an important mechanism of glomerular injury. The aim of this study is to determine whether the lectin pathway, a novel pathway of complement activation, is related to the pathogenesis of HSPN. Renal biopsy material from 10 patients with HSPN was studied immunohistochemically and examined for a clinicopathologic correlation. Serum levels of complement components, including mannose-binding lectin (MBL), and plasma levels of complement activation products were also evaluated in these patients and compared with levels in patients with immunoglobulin A (IgA) nephropathy or mesangial proliferative glomerulonephritis (GN) without IgA deposition (non-IgA GN). Glomerular deposition of components of the pathway, MBL and MBL-associated serine protease (MASP-1), as well as C3b/C3c, C5b-9, and C4-binding protein (C4-bp), was detected in 8 of 10 patients. Although no significant correlation was found between glomerular deposition of MBL/MASP-1 and histological or clinical findings, the biopsies on all patients with MBL/MASP-1 deposits were performed within 20 weeks from the onset of disease. Levels of plasma C4d, the activation fragment of C4, and C4-bp, a soluble regulatory protein of the pathway, were greater in patients with HSPN than in those with non-IgA GN. However, there was no difference in serum MBL levels between the three groups of patients (HSPN, IgA nephropathy, and non-IgA GN). These results suggest that complement activation through the lectin pathway was involved at the onset of HSPN, and this mechanism might be important in the disease pathogenesis.
- Published
- 2000
49. Contents Vol. 86, 2000
- Author
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Okan Bakınen, Gilbert Deray, Ken Okumura, Keiko Uchida, Ljubica Ðukanović, J.H. Park, Y.S. Haviv, Mitsuyoshi Furuhashi, Shou-Shan Chiang, Vladisav Stefanovic, Martin Ellbogen, E. Sedano, L. Grcevska, Sun Ae Yoon, Yuet-Ching Tay, Junne-Ming Sung, Hirotsugu Iwatani, Matt Koch, Toshiyuki Imasawa, Michael Field, Masahiko Nakamoto, Vincenzo Bellizzi, Jung-Kuei Pai, H. Pasantes-Morales, Yoshiyuki Hiki, Robert Dunlay, Yoshiaki Takemoto, Hideaki Yamabe, Johan W. de Fijter, Yutaka Kobayashi, Junko Tanaka, Ayşegül Örs Zümrütdal, Jyh-Gang Leu, B.K. Bang, Katsuo Suyama, Shigetake Sasayama, J. Möcks, A. Rodríguez-Cuartero, Toshika Okumiya, Minako Koike, Byung Kee Bang, Naoyuki Tamura, Hacı Veli Atalay, Adriaan M. Kamper, I. Villen, Chie Tomida, Heather J. Saunders, Kenji Tsuchida, Akira Kawashima, Giuseppe La Greca, Ming-Cheng Wang, Shu-May Lin, Tetuhiko Yoshida, Qu Huiqi, Yukitaka Maruyama, Hiroshi Nihei, Michihiro Gotoh, Kazuho Honda, Yasukazu Yamada, Shinichi Kakumu, Sohji Nagase, Elsie-C. Chan, Mutsuko Hidaka, Atsushi Ueda, Aysun Karabay Bayazit, Kazumasa Aoyagi, Masaya Yamato, Akio Koyama, Yoshihiro Matsumoto, Diana Ionova, Wei-Chi Lee, Slavenka Janković, Hiroshi Osawa, Tatsuo Hosoya, Qiu Mingcai, Takako Takita, Lin Shan, Shu-Yin Kuo, Gopala K. Rangan, Tsung Hsiu Wang, Richard J. Lund, Tatsuya Nakatani, Harutaka Yamada, Krasimira Sepetlieva, M. Pérez-Suarez, Jerome G. Porush, Aytül Noyan, Stefan Fründ, Predrag Vlahović, Weier Qi, P A Conz, Akihiko Kato, Hui Kyung Jeon, Jelena Marinkovic, Kazuhiro Okano, Akira Hishida, J.Y. Choi, Krystyna Szprynger, Brad Oldemeyer, Satoru Tsunoda, Takayuki Fujita, Hatice Bodur, G. Petruševska, Isao Ohsawa, Danuta Zwolińska, York Leng Yu, Arao Futenma, Hitoe Suzuki, G. Maschio, J.L. Pérez-Castrillón, Rich Jones, Gakusen Nishihara, Takanari Aoki, Maria Szczepańska, Danica Bukvić, Sumio Tateno, Masahiro Kakihara, M. Milovanceva-Popovska, Young Ok Kim, Kuddusi Cengiz, M. Arrabal-Martín, Yau-Huei Wei, Tein-Chung Lu, Toshiyuki Takahashi, David A. Vesey, Hiroshi Tatsumi, Kamen Tcachev, Keisuke Yamamoto, Eriya Kikawada, Monika Bulla, Vincenzo Terracciano, Jeng-Jong Huang, Haruo Tomonari, Junji Terao, Halil Uçan, Atsushi Fukatsu, Atsushi Yamauchi, Sun Jeong Lim, Robert Kleta, Kosaku Nitta, Atsushi Satomura, A. Zuluaga, Yoshiko Baba, Morito Endo, F.J. Pérez-Blanco, Hassan Izzedine, Paik-Seong Lim, M. Polenakovic, Mitsuaki Kaizuka, C.W. Yang, Takashi Uzu, A. Egon van der Bijl, Biagio Di Iorio, Chikao Yasunaga, Fumiko Tateyama, Aya Abe, Yiping Wang, Eun Jung Jun, Chan Joo Kim, Chang Hee Han, Ali Anarat, Eri Muso, Satoru Kuriyama, Izumi Amano, Aki Hirayama, Takanobu Sakemi, Y.M. Choo, Wey-Wen Jiang, G.B. Kim, Fen-Fen Chen, S. Morales Mulia, Anna Medyńska, Marina Mitić-Zlatković, Wako Yumura, I. Justo-Muradas, Carlo Crepaldi, Koichi Suzuki, Yasuhiro Chikamori, Kenichi Shirato, Naoto Miura, Y.S. Kim, Katsumi Takemura, Leendert C. Paul, Carol A. Pollock, A.J. Meares, Masatomo Yashiro, Vincent Launay-Vacher, Hiroyuki Ohi, David W. Johnson, E. Saracibar, David Harris, and Eberhard Kuwertz-Bröking
- Subjects
Traditional medicine ,business.industry ,Medicine ,business - Published
- 2000
50. Contents Vol. 81, 1999
- Author
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Hiroshi Inoue, Ramón Peces, T. Moriyama, Francisco J. Pérez-Blanco, Alexandre H. Campos, Kiichi Kubota, Shifra Sela, Jun Koshimura, Atushi Kurusu, Jayson Rapoport, Kiyoshi Izumino, M.L. Seco, A.F. Vanin, Antonio Rodríguez-Cuartero, Massimino Senatore, Tatuo Hosoya, Fumi Takemoto, Xiao Bo Huang, Manuela Födinger, Dagmar Schedler, S.K. Agarwal, Hiroji Kitazato, Cidio Chaimovitz, Kenji Nakayama, Hiroyuki Iida, Michael Furmanov, R. Lo Presti, Gere Sunder-Plassmann, Akira Yamada, MichaelJ. Hausmann, Hiroaki Io, Hiroshi Sato, Jun Igari, Ya Li Zheng, M. Sugita, Kimiko Kobayashi, Miwako Numata, L. Borque, Masaaki Nakayama, Tatsuo Hosoya, Hayakazu Nakazawa, Mami Kobata, Yoshindo Kawaguchi, Takuma Narita, E.N. Burgova, Yuko Nakagawa, Takao Saito, Kamal Hassan, Rafael A. Navascués, Joseph Manaster, Lourdes Morales-Camacho, Atsuko Maeda, Keitaro Yokoyama, M. Montana, Michele Buemi, Shuichi Kikuchi, Nestor Schor, Revital Shurtz-Swirski, G. Caimi, Sigeko Hara, Mitumine Fukui, F.U. Dzgoeva, Jie Liao, Fumio Ito, W H Hörl, Seiki Ito, Galina Shapiro, Hideo Hamaguchi, Shigeru Tsuchiya, Satoshi Kurihara, J. Alvarez, Ichiyu Shou, I.M. Kutyrina, Yutaka Kanamaru, Chiaki Hirano, Isao Shirato, Miguel Seco, MasaakiNakayama Nakayama, Ryoichi Ando, K.D. Salbiev, K. Oka, Batya Kristal, M. Irshad, Mari Tanaka, Hiroshi Toma, Francisco J. Miras-Parra, H. Nakahama, Masahiko Murata, Katsunori Suzuki, Toshio Hasegawa, Masanobu Takata, M. Caballero, Yasuhiko Tomino, Irith Weissman, Isao Kurihara, Hisako Yanagi, Shigeo Tomura, Kyouichi Tashiro, Mariko Tamano, Akihiro Futamura, A. Rus, S.C. Dash, Shaul M. Shasha, K. Obata, Shougo Kaneko, M. Sierra, Hiroyuki Ohi, Takeshi Hayashi, M. Horio, Yukihiko Takeda, F. Vaccaro, J Baltar, Robert Fritsche-Polanz, and B. Canino
- Subjects
Traditional medicine ,business.industry ,Medicine ,business - Published
- 1999
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