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Biogas generated from livestock manure is a renewable energy source and the digestate is used as a fertilizer. Moreover, dewatered biogas digestate can be used as a bedding material (recycled bedding material). The aims of the present study were to model a whole dairy system with a biogas plant using recycled bedding material and to assess the life cycle greenhouse gas (GHG) emissions. Emissions from the material flow of dairy cattle production, manure treatment and organic fertilizer application to on-farm crops were evaluated. In the emissions from organic fertilizer storage and recycled bedding material production, CH4 emission was decreased by 43.0%, and consequently the system with a biogas plant reduced total GHG emissions by 6.8% compared with conventional slurry storage and straw bedding. The use of recycled bedding material from a biogas plant has the potential to create a resource cycle and to be beneficial as a GHG mitigation strategy.

The rumen contains a complex microbial ecosystem that degrades plant materials, such as cellulose and hemicellulose. We herein reconstructed 146 nonredundant, rumen-specific metagenome-assembled genomes (MAGs), with ≥50% completeness and10% contamination, from cattle in Japan. The majority of MAGs were potentially novel strains, encoding various enzymes related to plant biomass degradation and volatile fatty acid production. The MAGs identified in the present study may be valuable resources to enhance the resolution of future taxonomical and functional studies based on metagenomes and metatranscriptomes.

We investigated the effects of supplementary calcium salt of fatty acid (CSFA) from linseed oil with different oil adsorbents on in vitro gas production and rumen fermentation characteristics in barley substrate condition. A non-supplementation treatment (CONT) and treatments of six products, CSFA without oil adsorbent (2.1 fatty acid/Ca molar ratio) and CSFAs with silica gel, zeolite, bentonite, diatomite, and vermiculite (2.8 fatty acid/Ca molar ratio), were prepared. The supplementary 2% and 4% CSFA with silica gel (+S) in the substrate reduced CH

Effects of partial replacement of fishmeal with termite (Hodotermopsis sjostedti) meal in chicken diets on BW, ADG, intake, and organ and meat weight were tested with Chunky broiler female chickens in two experiments. In Exp.1, 20 chickens (8 d of age and 239 ± 3.0 g BW initially) were allocated in individual pens and assigned one of the four diet treatments; commercial diet with fishmeal supplement (0.0%) and replacement of fishmeal with freeze-dried ground termite meal of 0.5%, 2.5%, and 5.0%. In Exp.2, 18 chickens (9 d of age and 309 ± 3.8 g BW initially) were assigned one of the three diet treatments; commercial diet (COM), 2.5% fishmeal supplement (FM), and 2.5% termite meal supplement (TM). At 21 d old, chickens were slaughtered, and meat and organ weight were measured. Treatment diet CP concentrations resulted in 29.7 and 27.9% DM for Exp.1 and Exp.2, respectively. There was no treatment effect (P>0.05) on BW, ADG, intake, feed conversion rate, meat (i.e., thigh, breast fillet, and inner breast fillet) weights, or organ (i.e., liver, heart, spleen, gizzard, proventriculus, and intestine) weight in both experiments. In Exp.1, empty cecum weight was greater (P=0.03) when termite meal was not included (0.37, 0.33, 0.31, and 0.31 % per shrunk BW for 0.0%, 0.5%, 2.5%, and 5.0%, respectively; SE=0.022). Similarly, whole cecum weight (0.38, 0.54, and 0.40 % per shrunk BW for COM, FM, and TM, respectively; SE=0.043) and cecum fill (0.08, 0.21, and 0.09 % per shrunk BW for COM, FM, and TM, respectively; SE=0.037) were greater (P=0.05) for FM than COM and TM in Exp.2. In conclusion, termite meal supplements did not have either favorable or adverse effects on the growth or weight of meat and organs except cecum weight. Further investigation is needed for cecum function and meat quality.

Termite (Hodotermopsis sjostedti) meal was evaluated as a potential protein alternative for chicken feed. After chemical compositions, mineral and vitamin contents, and amino acid concentrations of termite meal were analyzed, 20-layer male chickens (8 d old and 78±0.9 g initial BW) were allocated into individual pens and randomly assigned one of the 4 diet treatments; a commercial diet (CON) and inclusion of freeze-dried ground termite meal of 3.3%, 6.7%, and 10.0% to monitor growth and feed intake. At 11 d old, chickens were slaughtered and meat and liver weight and blood plasma parameters were measured. Termite meal composited 61.5, 15.0, 7.8, and 4.9% of CP, crude fat, crude fiber, and ash, respectively. Mineral contents were 1.58, 8.25, 4.38, 13.1, 1.49, and 2.18 g/kg for Na, P, Ca, K, Mg, Cl, respectively, and 321, 108, and 0.38 mg/kg for Fe, Cu, and Se, respectively. Amino acid concentrations were 2.76, 1.35, 2.11, 3.52, 3.03, 0.72, 1.99, 2.20, 2.88, 3.70, 4.18, 0.50, 6.10, 3.25, 2.75, 2.30, 0.57, and 3.52% for arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, valine, alanine, aspartic acid, cystine, glutamic acid, glycine, proline, serine, tryptophan, and tyrosine. There were quadratic effects on final BW (P=0.09; 109, 110, 113, and 110 g for CON, 3.3%, 6.7%, and 10.0%, respectively; SE=1.42), ADG (P=0.09; 10.1, 10.7, 11.6, and 10.4 g for CON, 3.3%, 6.7%, and 10.0%, respectively; SE=0.47), intake (P=0.02; 23, 21, 24, and 20 g for CON, 3.3%, 6.7%, and 10.0%, respectively; SE=1.16), and plasma Ca concentration (P=0.02; 10.1, 10.5, 10.0, and 10.3 mg/dL for CON, 3.3%, 6.7%, and 10.0%, respectively; SE=0.13), whereas main effects of the treatment for all the items tested were not detected (P>0.05). In conclusion, termite meal can be used as an alternative protein source for chicken feed but could have an adverse effect at 10% inclusion.

The objective of this study was to develop mathematical equations for describing the change in marbling in Japanese Black steers using longitudinal measurements. Serial ultrasound measurements were taken at 14, 16, 20, and 26 months of age and analyzed using an image analysis software. The longitudinal marbling measurements from the ultrasound images and carcasses were fitted into a nonlinear logistic curve. Data used for the analysis consisted of 749 steers that converged in nonlinear curve fitting and showed reasonable estimated parameters of the logistic curves. The average predicted mature beef marbling score (BMS) and maturation rate were 6.26 and 0.353, respectively, and the average maturity levels at 24 months of age were 83.9%. The heritability estimates for the predicted maturity traits were moderate, indicating that these traits may have potential for genetic improvement. There was a negative relationship between the expected progeny differences between carcass BMS and maturity traits, suggesting that genetic improvement by carcass BMS may lead to the selection of bulls with late maturity for marbling. The results indicate that ultrasound and model building for marbling can be useful tools to correctly select candidate bulls with high marbling in the early fattening period.

The objective of this study was to estimate genetic parameters for first calving reproductive traits and growth curve characteristics in Japanese Black cattle. The Gompertz growth function was fitted to body weight-age data to obtain the mature weight (MWT) and rate of maturing (ROM) of cows. Data of reproductive traits including the first service conception rate (CR) for heifers, age at the first calving (AFC), and gestation length for the first calving were collected. Records of 3,204 animals were used for analysis. Genetic parameters were estimated using a linear uni- and bivariate animal model. The heritability estimates were moderate (0.29 for ROM) and high (0.57 for MWT) for growth curve parameters and low (0.03-0.11) for reproductive traits. There was a negative genetic correlation between MWT and ROM (-0.26), suggesting that an animal with a faster ROM would show a lower MWT. CR was negatively correlated with MWT (-0.42) but significantly and positively correlated with ROM (0.91). There was a negative genetic correlation between AFC and MWT (-0.49). These results suggest that a heifer with a faster ROM and lower MWT would show a higher CR. Meanwhile, a heifer with a lower MWT would show a higher AFC.

Calcium salts of long-chain fatty acids (CSFA) from linseed oil have the potential to reduce methane (CH4) production from ruminants; however, there is little information on the effect of supplementary CSFA on rumen microbiome as well as CH4production. The aim of the present study was to evaluate the effects of supplementary CSFA on ruminal fermentation, digestibility, CH4production, and rumen microbiomein vitro. We compared five treatments: three CSFA concentrations—0% (CON), 2.25% (FAL) and 4.50% (FAH) on a dry matter (DM) basis—15 mM of fumarate (FUM), and 20 mg/kg DM of monensin (MON). The results showed that the proportions of propionate in FAL, FAH, FUM, and MON were increased, compared with CON (P < 0.05). Although DM and neutral detergent fiber expressed exclusive of residual ash (NDFom) digestibility decreased in FAL and FAH compared to those in CON (P < 0.05), DM digestibility-adjusted CH4production in FAL and FAH was reduced by 38.2% and 63.0%, respectively, compared with that in CON (P < 0.05). The generaRuminobacter,Succinivibrio,Succiniclasticum,Streptococcus,Selenomonas.1, andMegasphaera, which are related to propionate production, were increased (P < 0.05), whileMethanobrevibacterand protozoa counts, which are associated with CH4production, were decreased in FAH, compared with CON (P < 0.05). The results suggested that the inclusion of CSFA significantly changed the rumen microbiome, leading to the acceleration of propionate production and the reduction of CH4production. In conclusion, although further in vivo study is needed to evaluate the reduction effect on rumen CH4production, CSFA may be a promising candidate for reduction of CH4emission from ruminants.

Genomic imprinting should be considered in animal breeding systems to avoid lead in bias in genetic parameter estimation. The objective of this study was to clarify the effects of pedigree information on imprinting variances for carcass traits and fatty acid composition in Japanese Black cattle. Carcass records [carcass weight, rib eye area, rib thickness (RT), subcutaneous fat thickness and beef marbling score (BMS)] and fatty acid composition were obtained for 11,855 Japanese Black feedlot cattle. To estimate and compare the imprinting variances for the traits, two imprinting models with different pedigree information [the sire-dam gametic relationship matrix (Model 1) and the sire-maternal grandsire (MGS) numerator relationship matrix (Model 2)] were fitted. The ratio of the imprinting variance to the total additive genetic variance for RT (6.33%) and BMS (19.00%) was significant in Model 1, but only that for BMS (21.09%) was significant in Model 2. This study revealed that fitting the sire-MGS model could be useful in estimating imprinting variance under certain conditions, such as when restricted pedigree information is available. Furthermore, the present result suggested that the maternal gametic effects on BMS should be included in breeding programmes for Japanese Black cattle to avoid selection bias caused by imprinting effects.

[This corrects the article DOI: 10.1371/journal.pone.0223447.].

Animal welfare and environmental impacts have been emphasized in the sustainable production of livestock. Labels are useful tools for clearly providing such attribute information to consumers. The aim of this study was to evaluate how human values influence consumer segments for beef with information on animal welfare and environmentally friendly production. Using a choice experiment, we examined whether animal welfare and environmentally friendly labels, country of origin and price impact consumer choice. As results, five heterogeneous consumer classes were identified using a latent class model: label conscious, domestic beef preferring, price conscious, animal welfare preferring and not interested in production method. Almost 90% of consumers were interested in and willing to pay for beef with animal welfare or environmentally friendly label. The classes with significant preferences for such labeled beef were affected by "openness to change", "self-enhancement" and "security". Improving consumer attitudes and strengthening consumer perception towards labeled beef by marketers and policy makers will be required.

The sizes of body parts often co-vary through exponential scaling, known as allometry. The evolution of allometry is central to the generation of morphological diversity. To make inferences regarding the evolved responses in allometry to natural and artificial selection, we compared allometric parameters (slope and intercept) among seven species and breeds of domestic bovids using cross-sectional ontogenetic data and attempted to interpret the differences in these parameters. The allometric slopes were not different among some species, whereas those between breeds within species were, indicating that the slopes were typically invariant but could be changed under strong, specific selection. With the exception of yak, the differences in the intercept independent of the slopes (the alternative intercept) among species might better correspond to their divergence times than the differences in allometric slope, and the remarkably higher alternative intercept found in yaks can be explained by their unique morphological evolution. These findings provide evidence that differences in the alternative intercept can retain traces of the phylogenetic changes derived from differentiation and evolution., ウシ科家畜における体重と胸囲の関係の種間比較 --自然淘汰や人為選抜による形態の進化--. 京都大学プレスリリース. 2017-07-12.

The improvement of farm animal welfare through management practices has become important despite the fact that there is a conflict between animal welfare and production efficiency. It may be possible to find associations between production and management practices related to animal welfare, such as management strategies to enhance both production and animal welfare. The objective of the present study was to investigate the effect of different fattening management practices with respect to animal welfare on the carcass characteristics in Japanese beef cattle fattening systems at the farm level. The face-to-face interview survey on welfare-related management practices was conducted with 30 beef fattening farmers who sold more than 5 animals from July 2014 to March 2015 in the carcass market in Shiga Prefecture. The best linear unbiased predictors of each farm (the so-called farm BLUP) for carcass traits (carcass weight, CWT; marbling score, BMS; longissimus muscle area, LMA; rib thickness, RT; and subcutaneous fat thickness, SFT) and carcass unit price (CUP) were estimated from the data collected at the carcass market. The effect of management practices on farm BLUPs for carcass traits and CUP were tested using one-way ANOVA. For management practices, there were significant effects of routine claw trimming on farm BLUP for SFT (P

The estimation of energy expenditure (EE) of grazing animals is of great importance for efficient animal management on pasture. In the present study, a method is proposed to estimate EE in grazing animals based on measurements of body acceleration of animals in combination with the conventional Agricultural and Food Research Council (AFRC) energy requirement system. Three-dimensional body acceleration and heart rate were recorded for tested animals under both grazing and housing management. An acceleration index, vectorial dynamic body acceleration (VeDBA), was used to calculate activity allowance (AC) during grazing and then incorporate it into the AFRC system to estimate the EE (EE derived from VeDBA [EE]) of the grazing animals. The method was applied to 3 farm ruminant species (7 cattle, 6 goats, and 4 sheep). Energy expenditure based on heart rate (EE) was also estimated as a reference. The result showed that larger VeDBA and heart rate values were obtained under grazing management, resulting in greater EE and EE under grazing management than under housing management. There were large differences between the EE estimated from the 2 methods, where EE values were greater than EE (averages of 163.4 and 142.5% for housing and grazing management, respectively); the EE was lower than the EE, whereas the increase in EE under grazing in comparison with housing conditions was larger than that in EE. These differences may have been due to the use of an equation for estimating EE derived under laboratory conditions and due to the presence of the effects of physiological, psychological, and environmental factors in addition to physical activity being included in measurements for the heart rate method. The present method allowed us to separate activity-specific EE (i.e., AC) from overall EE, and, in fact, AC under grazing management were about twice times as large as those under housing management for farm ruminant animals. There is evidence that the conventional energy system can predict fasting metabolism and the AC of housed animals based on accumulated research on energy metabolism and that VeDBA can quantify physical activity separately from other factors in animals on pasture. Therefore, the use of the VeDBA appears to be a precise way to predict activity-specific EE under grazing conditions, and the method incorporating acceleration index data with a conventional energy system can be a simple and useful method for estimation of EE in farm ruminants on pastures.

Evaluation of animal behavior provides information on health and well-being in animals. In this preliminary study, we investigated the effectiveness of an accelerometry approach to evaluate changes in health status of cattle by combining physical activity quantified by dynamic body acceleration (DBA) and qualitative behavioral classification data. Although feeding low vitamin A (VA) diets to fattening cattle is a popular technique to enhance marbling of beef in Japan, the effects of VA restriction on behavior of fattening cattle have been unclear. Therefore, the effect of VA restriction on behavioral change in feedlot steers was assessed by the approach as an example of evaluation of the change in health status. Ten Japanese Black fattening steers were divided into VA restricted (RES) and supplemented (SUP) groups (five in each group). The RES steers were fed lower VA diets than SUP from 11 to 20 months of age. The measurements of blood VA concentrations, accelerations and classified behaviors of the steers were conducted at 18 and 24 months of age. An accelerometer was fitted dorsally to each steer and three axial accelerations were measured at 10 Hz. The raw acceleration data was processed to calculate vectorial DBA (VeDBA) per second. Behavior of the steers was recorded for 8.5 h on one day and visually classified per second as lying, standing, walking or feeding. As the results, the blood VA concentration at 18 months of age was lower in RES than SUP (P

Biochemical values and mineral concentrations in blood plasma were investigated to evaluate the statuses of health and mineral nutrition among yaks in Mustang District, Nepal. In total, 118 plasma samples of female yaks collected in April and September/October of 2013-2015 were offered. Seventy-four percent of yaks showed lower plasma total-cholesterol concentrations than the lowest limit of reference range (100 mg/dL) and the values in spring (83.41 mg/dL) were lower (P < 0.05) than those in autumn (95.05 mg/dL). All the yaks had lower plasma albumin concentrations than the lowest limit of reference range (3.0 g/dL) and 66% of yaks showed lower plasma inorganic phosphorus concentrations than the critical level of phosphorus deficiency (4.5 mg/dL). Thirty-five percent of yaks showed lower plasma calcium concentrations than the lowest limit of normal range (8 mg/dL) and the concentrations were lower in spring than in autumn (P < 0.01). Seventy-five percent of yaks presented lower copper concentrations than the critical level (0.65 mg/L) and the concentrations were lower in spring than in autumn (P < 0.01). Since the low plasma total-cholesterol might have indicated shortage of dry matter and energy intake, attention should be paid to the nutritional statuses of energy, phosphorus, calcium and copper in winter and early spring.

We examined the in vitro fermentation characteristics and in situ dry matter (DM) and crude protein (CP) degradability of tamarind kernel powder extract residue (TKPER), a by-product of polysaccharides thickener processing. Two types of TKPER (I and II), of which the CP and neutral detergent fiber organic matter basis contents (%) were 41.4 and 42.0 and 1.4 and 0.5, respectively, were compared with dry heat soybean (SB), soybean meal (SBM) and dry soybean curd residue (SBCR). The TKPERs had significantly lower in vitro gas production compared to the other products at each observation time (P

Wine lees are by-products of wine-manufacturing, but the usefulness as animal by-product feed has not yet been revealed. The present study was aimed to investigate the usability of wine lees as feed for ruminants in fattening conditions by in vitro and in vivo trials. In in vitro experiments (in vitro I and II), the following four treatments were prepared: 100% dry matter (DM) rolled barley as a control (WL0), and replaced 7.5% DM, 15.0% DM, and 22.5% DM of wine lees as three treatments (WL7.5, WL15, WL22.5, respectively). A linear decrease in gas production was observed at 48 h after incubation with an increase in the percentage of wine lees in the substrate (P 0.05). As wine lees increased, the proportion of ruminal propionate at 4 h after feeding and plasma malondialdehyde, which is one of the oxidative stress markers, were decreased (P

This study was aimed at investigating the local livestock biomass volume for dairy farms in Town A, located in eastern Hokkaido, Japan, and at presenting a model of a biogas plant that enables maximizing the use of the available livestock biomass. Using a dairy farm with 250 animals in Town A as a model for a biogas plant based on dry-type methane fermentation system (dry-type biogas plant), we set the operational conditions to an average hydraulic retention time of 20 days, digestion temperature of 55°C, and methane gas yield of 0.12 Nm3 CH4/kg VSA. We compared the biogas production of our presented model with that of a wet-type biogas plant with the same number of animals. The results showed that the dry-type biogas plant produced biogas at 859 Nm3/day, while the wet-type biogas plant produced biogas at 666 Nm3/day. These results indicate that introducing dry-type biogas plants in all dairy farms in Town A would potentially enable semi-solid livestock manure to be processed, which is not amenable to ordinary composting, in addition to the conventional processes being carried out through biogas plants, as well as lead to an increase in the amount of biogas production., International Conference of Biomass and Bioenergy 19-20 August 2019, West Java, Indonesia

Variances caused by the differential expression of paternally and maternally imprinted genes controlling carcass traits in Japanese Black cattle were estimated in this study. Data on marbling score (BMS), carcass weight, rib thickness, rib-eye area (REA) and subcutaneous fat thickness (SFT) were collected from a total of 13,115 feedlot steers and heifers in a commercial population. A sire-maternal grandsire model was used to analyse the data, and then, imprinting parameters were derived by replacing the genetic effect of the dam with the effect of the maternal grandsire in the imprinting model to calculate the genetic parameter estimates. The proportions of the total genetic variance attributable to imprinted genes ranged from 8.7% (SFT) to 35.2% (BMS). The remarkably large imprinting variance of BMS was mainly contributed by maternally expressed inheritance because the maternal contribution of the trait was much larger than that of the paternal trait. The parent-of-origin effect originating from maternal gene expression was also observed for REA. The results suggested the existence of genomic imprinting effects on the traits of the Japanese Black cattle. Hence, the parent-of-origin effect should be considered for the genetic evaluation of Japanese Black cattle in breeding programmes.

Mother liquor (ML) is monosodium glutamate by-product and contains much crude protein (CP). The objective of this study was to investigate the effect of supplementation of two types of MLs having different chemical compositions on in vitro ruminal fermentation characteristics. ML1 had 74% organic matter (OM) and 70% CP and ML2 had 85% OM and 83% CP on a dry matter (DM) basis. The experiments were conducted using 0.5 gDM rolled barley or ryegrass straw as substrates. Urea and freeze-dried MLs were added at three levels of nitrogen to be isonitrogenous. The ML1 treatment increased gas production compared with the ML2 treatment in barley substrate condition (p

We assessed the nitrogen (N) and phosphorus (P) flows in intensified livestock production systems by investigating nutrient budgets and cycling in the basin of Dianchi Lake, one of the most eutrophic lakes in China. We conducted field surveys based on feed samplings and interviews of livestock farmers. The N and P in local and external feeds, animal body retentions, animal products and excretions were calculated at the individual level for dairy cattle, fattening pigs, breeding sows, broilers and laying hens. The N and P flows in the total livestock production system in the area were estimated by multiplying the individual N and P budgets by the number of animals. For the dairy and fattening pig productions, N and P supplied from local crops or by-products accounted for large parts of the inputs. For the other livestock categories, most of the N and P inputs depended on external resources. The N and P outputs through animal manure into the cropland were 287 and 66 kg/ha/year, respectively, which were higher than the N and P inputs into the livestock production systems from the cropland. The N and P loads from manure should be reduced for the establishment of sustainable agricultural production systems.

Four Thai native cattle were used in a 4 × 4 Latin square design experiment to evaluate the availability of desalted mother liquor (DML) as replacement of salt in concentrate. Each cattle was assigned to one of the following concentrate feeding treatments: C1, 1% NaCl was added as salt; C2, 2% NaCl was added as salt; D1, 1% NaCl was replaced by DML; D2, 2% NaCl was replaced by DML, on a dry matter (DM) basis. The animals were fed rice straw and experimental concentrates (40:60) at 1.9% of body weight on a DM basis, daily. Acid detergent fiber expressed exclusive of residual ash (ADFom) digestibility in DML treatment was higher than salt treatment (p < .05) and D2 feeding showed the highest value (60.8%). There were no significant differences in blood metabolites, nitrogen retention, ruminal ammonia nitrogen, methane emission or energy efficiency among treatments. Molar percent of acetate on volatile fatty acids in rumen fluid 4 hr post-feeding tended to be higher in DML treatment than salt treatment (p = .08). The results indicated that adding DML could improve ADFom digestibility and salt could be replaced by DML up to 2% as NaCl in concentrate without adverse effects on nitrogen balance, rumen conditions, blood metabolites and methane emission.

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate the availability of two types of winery wastes, winery sediment and grape pomace, as ruminant feeds possessing antioxidant activities. Each wether was assigned to one of the following four treatments: (i) 75 g/kg winery sediment (WS) on a dry matter (DM) basis; (ii) 166 g/kg DM winery grape pomace (WP); (iii) control diet (CD; 17 g/kg DM soybean meal);and (iv) only tall fescue hay (TFH; no additive). Winery sediment and grape pomace had high levels of polyphenols and of radical scavenging activities. Feeding with winery sediment and grape pomace did not negatively affect the intake, but it depressed crude protein (CP) digestibility compared with CD (P = 0.052 and P

Four wethers were used in a 4 × 4 Latin square design experiment to evaluate in vivo digestibility of total mixed ration (TMR) silage with food by-products for dairy cows, and the ruminal condition and nitrogen (N) balance were examined. Five by-products (i.e. potato waste, noodle waste, soybean curd residue, soy sauce cake and green tea waste) were obtained. Four types of TMR silage were used: control (C) containing roughage and commercial concentrate, T1:20% and T1:40% containing the five by-products replacing 20% and 40% of the commercial concentrate on a dry matter (DM) basis, respectively, and T2:40% containing three by-products (potato waste, noodle waste and soybean curd residue) replacing 40% of the commercial concentrate on a DM basis. The ingredients were mixed and preserved in oil drum silos for 4 months. The TMR silages showed 4.02–4.44% and 1.75–2.19% for pH and lactic acid contents, respectively. The digestibility of DM and neutral detergent fiber, and total digestible nutrient content were higher (P

Postnatal mortality (PM) of calves is an economically important trait, because a high mortality rate of calves decreases farm income and significantly increases production costs. In spite of the economic importance of PM, genetic studies on PM have been scarce for Japanese Black cattle. The objectives of this study were to estimate the direct and maternal heritability for PM using a Bayesian threshold model with Gibbs sampling in Japanese Black cattle. The original data consisted of 43 723 records of animals born from January 2005 to June 2006. Four binary traits of mortality were considered: D1-14, D15-60, D61-180 and D1-180 with numbers indicating the period of risk in days after birth. Direct heritabilities for PM ranged from 0.12 to 0.31 and maternal heritabilities for PM were lower than the direct heritabilities and ranged from 0.02 to 0.16. Direct genetic correlations between D1-14 and D15-60, between D15-60 and D61-180, and between D1-14 and D61-180 were 0.501, 0.689 and 0.062, respectively. Direct genetic correlations of birth weight with D1-14, D15-60, D61-180 and D1-180 were 0.089, 0.482, 0.434 and 0.394, respectively. These results indicated that different genes were responsible for early and late PM and early PM (D1-14) can be genetically reduced without influencing birth weight.