11 results on '"Hiromi Murase"'
Search Results
2. New Experiments and Efforts for the Treatment of Anal Fistula
- Author
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Risa Nishio, Hiromi Murase, Satomi Furukawa, Tetsuo Yamana, Takashi Fujimoto, Shunsuke Motegi, Kinya Okamoto, Emi Yamaguchi, Tetsuya Kudai, Emi Inoue, Satoka Nasu, and Yuuki Azuma
- Subjects
Anal fistula ,medicine.medical_specialty ,business.industry ,Gastroenterology ,medicine ,Surgery ,medicine.disease ,business - Published
- 2021
3. Effect of discontinuing antithrombotic drugs in patients undergoing percutaneous endoscopic gastrostomy
- Author
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Shojiro Miyazaki, Kei Iwasaki, Hiromi Murase, Godai Yoneda, and Toru Narita
- Subjects
medicine.medical_specialty ,business.industry ,Mechanical Engineering ,Percutaneous endoscopic gastrostomy ,medicine.medical_treatment ,Antithrombotic ,medicine ,Energy Engineering and Power Technology ,In patient ,Management Science and Operations Research ,business ,Surgery - Published
- 2019
4. Progranulin increases phagocytosis by retinal pigment epithelial cells in culture
- Author
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Masamitsu Shimazawa, Hiromi Murase, Kazuhiro Tsuruma, Hideaki Hara, and Yoshiki Kuse
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,media_common.quotation_subject ,Phagocytosis ,Biology ,Lipofuscin ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,0302 clinical medicine ,Growth factor receptor ,mental disorders ,medicine ,Internalization ,media_common ,Retina ,Retinal pigment epithelium ,Retinal ,Photoreceptor outer segment ,eye diseases ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,030221 ophthalmology & optometry ,sense organs - Abstract
Retinal pigment epithelium (RPE) cells take part in retinal preservation, such as phagocytizing the shed photoreceptor outer segments (POS), every day. The incomplete phagocytic function accelerates RPE degeneration and formation of the toxic by-product lipofuscin. Excessive lipofuscin accumulation is characteristic of various blinding diseases in the human eye. Progranulin is a cysteine-rich protein that has multiple biological activities, and it has a high presence in the retina. Progranulin has been recognized to be involved in macrophage phagocytosis in the brain. The purpose of this study is to determine whether progranulin influences phagocytosis by RPE cells. All experiments were performed on primary human RPE (hRPE) cells in culture. pHrodo was used to label the isolated porcine POS, and quantification of pHrodo fluorescence was used to determine the degree of phagocytosis. Western blotting and immunohistochemistry of key proteins involved in phagocytosis were used to clarify the mechanism of progranulin. Progranulin increased RPE phagocytosis in hydrogen peroxide-treated and nontreated RPE cells. The phosphorylated form of Mer tyrosine kinase, which is important for POS internalization, was significantly increased in the progranulin-exposed cells. This increase was attenuated by SU11274, an inhibitor of hepatic growth factor receptor. Under the oxidative stress condition, exposure to progranulin led to an approximately twofold increase in integrin alpha-v, which is associated with the first step in recognition of POS by RPE cells. These results suggest that progranulin could be an effective stimulator for RPE phagocytosis and could repair RPE function. © 2017 Wiley Periodicals, Inc.
- Published
- 2017
5. A case of successful balloon dilation in single-balloon enteroscopy and avoidance of surgery in a patient with intestinal obstruction accompanied by severe adhesions from multiple surgeries
- Author
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Kei Iwasaki, Hiromi Murase, Toru Narita, Shojiro Miyazaki, and Godai Yoneda
- Subjects
medicine.medical_specialty ,business.industry ,Mechanical Engineering ,Balloon dilation ,Energy Engineering and Power Technology ,Medicine ,Single-Balloon Enteroscopy ,Management Science and Operations Research ,business ,Surgery - Published
- 2018
6. Progranulin increases phagocytosis by retinal pigment epithelial cells in culture
- Author
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Hiromi, Murase, Kazuhiro, Tsuruma, Yoshiki, Kuse, Masamitsu, Shimazawa, and Hideaki, Hara
- Subjects
Phagocytosis ,Swine ,Animals ,Humans ,Epithelial Cells ,Retinal Pigment Epithelium ,Cells, Cultured - Abstract
Retinal pigment epithelium (RPE) cells take part in retinal preservation, such as phagocytizing the shed photoreceptor outer segments (POS), every day. The incomplete phagocytic function accelerates RPE degeneration and formation of the toxic by-product lipofuscin. Excessive lipofuscin accumulation is characteristic of various blinding diseases in the human eye. Progranulin is a cysteine-rich protein that has multiple biological activities, and it has a high presence in the retina. Progranulin has been recognized to be involved in macrophage phagocytosis in the brain. The purpose of this study is to determine whether progranulin influences phagocytosis by RPE cells. All experiments were performed on primary human RPE (hRPE) cells in culture. pHrodo was used to label the isolated porcine POS, and quantification of pHrodo fluorescence was used to determine the degree of phagocytosis. Western blotting and immunohistochemistry of key proteins involved in phagocytosis were used to clarify the mechanism of progranulin. Progranulin increased RPE phagocytosis in hydrogen peroxide-treated and nontreated RPE cells. The phosphorylated form of Mer tyrosine kinase, which is important for POS internalization, was significantly increased in the progranulin-exposed cells. This increase was attenuated by SU11274, an inhibitor of hepatic growth factor receptor. Under the oxidative stress condition, exposure to progranulin led to an approximately twofold increase in integrin alpha-v, which is associated with the first step in recognition of POS by RPE cells. These results suggest that progranulin could be an effective stimulator for RPE phagocytosis and could repair RPE function. © 2017 Wiley Periodicals, Inc.
- Published
- 2016
7. Analysis and characterization of anthocyanins and carotenoids in Japanese blue tomato
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Hiromi Murase, Masamitsu Shimazawa, Emi Ooe, Kenjirou Ogawa, Hideaki Hara, Hiroyuki Tada, Tadashi Horiuchi, and Kazuhiro Tsuruma
- Subjects
0301 basic medicine ,Spectrometry, Mass, Electrospray Ionization ,Blue tomato ,Applied Microbiology and Biotechnology ,Biochemistry ,Antioxidants ,Analytical Chemistry ,Cell Line ,Anthocyanins ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Lycopene ,Solanum lycopersicum ,Petunidin ,Animals ,Food science ,Benzothiazoles ,Molecular Biology ,Carotenoid ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,030109 nutrition & dietetics ,Plant Extracts ,fungi ,Organic Chemistry ,food and beverages ,General Medicine ,Hydrogen Peroxide ,Malvidin ,Carotenoids ,chemistry ,Polyphenol ,030220 oncology & carcinogenesis ,Anthocyanin ,Fruit ,Delphinidin ,Sulfonic Acids ,Biotechnology ,Photoreceptor Cells, Vertebrate - Abstract
Tomato (Solanum lycopersicum) is rich in anthocyanins, which are polyphenolic pigments. This study aimed to analyze and characterize the anthocyanin composition in cultivated blue tomato in Japan. The extracts of peel, seed, and pulp of tomatoes were purified following which anthocyanins and lycopene contents were analyzed using high-performance liquid chromatography and electrospray ionization mass spectrometry. Eleven types of anthocyanins were identified, including delphinidin, petunidin, and malvidin. Further, the antioxidant activity of anthocyanins was evaluated using 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt radical quenching assays and electron spin resonance. “Blue tomato” extracts exert antioxidant activity. Thus, we showed that petunidin was present in the “blue tomato” peel while lycopene was present in the peel and pulp. Additionally, the blue tomato peel extract was found to significantly inhibit H2O2-induced cell death in vitro. This is the first study on cell protective effects of Japanese blue tomato extract and petunidin in murine photoreceptor cells.
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- 2015
8. Warrior Princesses, How Far will You Go? : Transitions in the Characterization of Female Figures in Japanese 'Anime
- Author
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Hiromi, Murase, フィールドレポート, Field Report, 山口大学 非常勤講師, and Yamaguchi University, Adjunct Instructor
- Published
- 2006
9. Intraocular Pressure-Lowering Efficacy of Latanoprost in Patients with Normal-Tension Glaucoma or Primary Open-Angle Glaucoma
- Author
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Yoshiaki Kitazawa, Hiromi Murase, Toru Taniguchi, Yuji Tamada, and Tetsuya Yamamoto
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Male ,medicine.medical_specialty ,Intraocular pressure ,genetic structures ,Open angle glaucoma ,Adrenergic beta-Antagonists ,Glaucoma ,Dinoprost ,Tonometry, Ocular ,chemistry.chemical_compound ,Pharmacotherapy ,Ophthalmology ,Normal tension glaucoma ,Humans ,Medicine ,Pharmacology (medical) ,Prospective Studies ,Latanoprost ,Antihypertensive Agents ,Intraocular Pressure ,Pharmacology ,business.industry ,Middle Aged ,medicine.disease ,eye diseases ,Low Tension Glaucoma ,chemistry ,Unoprostone ,Prostaglandins F, Synthetic ,Drug Evaluation ,Drug Therapy, Combination ,Female ,sense organs ,Ophthalmic Solutions ,business ,Glaucoma, Open-Angle ,medicine.drug - Abstract
We investigated the intraocular pressure (IOP)-lowering potential of latanoprost in patients with normal-tension glaucoma (NTG) or primary open-angle glaucoma (POAG). This prospective study included 59 NTG and 20 POAG patients treated with the following four dosing regimens of latanoprost: patients on no previous medication received latanoprost as initial therapy (Group 1, n=31), patients on beta-blocker therapy received latanoprost as adjunctive therapy (Group II, n=9), patients on unoprostone monotherapy were switched to latanoprost monotherapy (Group III, n=14), and patients previously on dual therapy with isopropyl unoprostone and beta-blocker were switched to a combined treatment of latanoprost and beta-blocker (Group IV, n=25). IOP significantly decreased 8 weeks after initiation of latanoprost therapy by 19.9% in Group I, 20.5% in Group II, 16.6% in Group III, and 12.2% in Group IV. In Groups I and II, there was a significant positive correlation between the magnitude of IOP reduction induced by latanoprost and the IOP level before latanoprost therapy. The IOP level before latanoprost therapy is a contributing factor in the IOP-lowering efficacy of latanoprost. Latanoprost is more effective in lowering IOP than isopropyl unoprostone.
- Published
- 2001
10. The Effects of Brazilian Green Propolis against Excessive Light-Induced Cell Damage in Retina and Fibroblast Cells
- Author
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Hiromi Murase, Kenji Ichihara, Mamoru Kakino, Masamitsu Shimazawa, Kazuhiro Tsuruma, and Hideaki Hara
- Subjects
MAPK/ERK pathway ,Article Subject ,Traditional medicine ,business.industry ,lcsh:Other systems of medicine ,Propolis ,medicine.disease ,lcsh:RZ201-999 ,Molecular biology ,chemistry.chemical_compound ,medicine.anatomical_structure ,Complementary and alternative medicine ,chemistry ,Extracellular ,Medicine ,Viability assay ,Propidium iodide ,sense organs ,business ,Fibroblast ,Cell damage ,Intracellular ,Research Article - Abstract
Background. We investigated the effects of Brazilian green propolis and its constituents against white light- or UVA-induced cell damage in mouse retinal cone-cell line 661W or human skin-derived fibroblast cells (NB1-RGB).Methods. Cell damage was induced by 3,000lx white light for 24 h or 4/10 J/cm2UVA exposure. Cell viability was assessed by Hoechst33342 and propidium iodide staining or by tetrazolium salt (WST-8) cell viability assay. The radical scavenging activity of propolis induced by UVA irradiation in NB1-RGB cells was measured using a reactive-oxygen-species- (ROS-) sensitive probe CM-H2DCFDA. Moreover, the effects of propolis on the UVA-induced activation of p38 and extracellular signal-regulated kinase (ERK) were examined by immunoblotting.Results. Treatment with propolis and two dicaffeoylquinic acids significantly inhibited the decrease in cell viability induced by white light in 661W. Propolis and its constituents inhibited the decrease in cell viability induced by UVA in NB1-RGB. Moreover, propolis suppressed the intracellular ROS production by UVA irradiation. Propolis also inhibited the levels of phosphorylated-p38 and ERK by UVA irradiation.Conclusion. Brazilian green propolis may become a major therapeutic candidate for the treatment of AMD and skin damage induced by UV irradiation.
- Published
- 2013
11. TUDCA Promotes Phagocytosis by Retinal Pigment Epithelium via MerTK Activation
- Author
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Kazuhiro Tsuruma, Masamitsu Shimazawa, Hiromi Murase, and Hideaki Hara
- Subjects
Retinal degeneration ,Swine ,Phagocytosis ,Blotting, Western ,Retinal Pigment Epithelium ,C-Mer Tyrosine Kinase ,Phagocytic dysfunction ,Taurochenodeoxycholic Acid ,chemistry.chemical_compound ,Proto-Oncogene Proteins ,medicine ,Animals ,Humans ,Rhythmic process ,Cells, Cultured ,Aged ,Cell Death ,c-Mer Tyrosine Kinase ,Chemistry ,Receptor Protein-Tyrosine Kinases ,Tauroursodeoxycholic acid ,MERTK ,Retinal Photoreceptor Cell Outer Segment ,medicine.disease ,Photoreceptor outer segment ,Cell biology - Abstract
Purpose Renewal and elimination of the aged photoreceptor outer segment (POS) by RPE cells is a daily rhythmic process that is important for long-term vision. Phagocytic dysfunction results in photoreceptor cell death. Tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, is known to show neuroprotective effects in stroke, neurological diseases, and retinal degeneration models. In this study, we investigated the effects of TUDCA on retinal phagocytosis. Methods We used pHrodo-succinimidyl ester (SE), a pH-sensitive fluorescent dye, to label the POS for monitoring phagocytosis. After ingestion, the intensity of pHrodo fluorescence increases because of the pH changes inside the liposome. An RPE cell line, ARPE-19, and primary human RPE cells were used to investigate the hydrogen peroxide (H2O2)-induced disruption of phagocytosis in the pH-sensitive fluorescence POS phagocytosis assay. Additionally, we examined whether TUDCA could promote phagocytic function. Results The intensity of pHrodo light emission increased in a time-dependent manner. Tauroursodeoxycholic acid enhanced phagocytosis of POS and protected against H2O2-induced phagocytic dysfunction. It also promoted phagocytic function via activation of Mer tyrosine kinase receptor (MerTK), which is known to have a key role in the physiological renewal of POS. Conclusions These results suggest that TUDCA activates MerTK, which is important for phagocytosis of POS. Tauroursodeoxycholic acid may represent a new therapeutic option for the treatment of retinal diseases.
- Published
- 2015
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