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Survey on the wild genetic resources of hardy kiwifruit (Actinidia arguta) in Japan was conducted to determine the ploidy variation and its geographic distribution. Among the 127 wild plants collected from different geographic locations, 15 plants were diploid, 87 were tetraploid, and 22 were hexaploid. Additionally, 2 plants were heptaploid and one plant was octaploid. The tetraploid plants were distributed all over the country, whereas the diploid and hexaploid plants were geographically localized, in the warm Pacific hill areas of the south western part and in the deep-snow region of the mid-northern part of Honshu, respectively. The diploid plants could be clearly distinguished from other plants with ploidy variation by the morphological characteristics of the leaf and fruit. Hexaploid plants showed a relatively larger L/D ratio of the leaf blade, a greenish petiole, and pubescence on the petiole and lower leaf vein, whereas the tetraploid plants exhibited a reddish petiole and callose hairs on the vein of the lower leaf surface. Fruit shape of the tetraploid plants varied largely, from round to ellipsoidal, whereas that of the hexaploid plants was mostly ellipsoidal. These results indicate that the hexaploid plants of A. arguta as well as the diploid and tetraploid ones, naturally grow in a certain size of population in the restricted region of Japan.

We experienced a case of pulmonary embolism following laparoscopic cholecystectomy probably due to pneumoperitonum. A 46-year-old woman, who had a laparoscopic cholecystectomy, began complaining of dyspnea about 24 hours after the operation. Blood gas analysis revealed severe hypoxemia with PaO2 level of 47.6mmHg. These physical signs and clinical data were indicative of pulmonary embolism, so anticoagulant therapy with 10, 000unit/day of heparin was initiated. Pulmonary sintigram at the onset demonstrated segmental perfusion defect of Lt. S8 and Rt. S10. After the initiation of the therapy her condition improved markedly. A suspecion has been aroused that pneumoperitonum may induse deep vein thrombosis causing pulmonary embolism since the introduction of laparoscopic cholecystectomy. For this disease, early detection and early treatment are determinative factors for prognosis. So it should be considered as one of the most critical complications after laparoscopic surgery and cautious strategy for prevention is important in high-risk cases.

胃切除術後早期吻合部狭窄症状を改善する目的で茯苓飲を投与し, その作用機序について実験的研究を行った. 胃切除術を施行した64例のうち25例 (39%) で狭窄症状を訴え, 14例に茯苓飲を投与し (A群), 11例は投与しなかった (B群). A群14例のうち12例 (85.7%) において1週間以内に症状の消失を認めた. 症状消失期間はA群は5日間, B群は13.2日と茯苓飲投与により有意に短縮した. 造影所見の残胃拡張・吻合部通過の程度に有意差は認めなかった. ラットの腺胃1/2切除後, Billroth I法で再建し, 茯苓飲・六君子湯・水道水を投与し, 5日後の残胃の容量・pH・病理組織を検討した. 残胃の容量・pHに差はなかった. 茯苓飲投与により有意に残胃側吻合部の粘膜下層の浮腫の軽減を認めた. 以上より, 茯苓飲投与により吻合部の浮腫を軽減することにより, 自覚症状を速やかに改善するものと思われた.

The structures of two novel isopentyl phloroglucinol dimers, designated as sideroxylonals A and B were deduced on the basis of proton magnetic resonance, 13C-nuclear magnetic resonance and mass spectral evidences.

A novel nucleic acid model that possessed 5'-amino-5'-deoxyuridine at alpha- and gamma-position of L-glutamic acid through amide linkage using 5'-amino group was synthesized and the conformation and the hybridization properties were studied. The complex of alpha-PRNA with complementary DNA/RNA was more stable than the corresponding natural duplex in the absence of borate. Its recognition ability was however lost when borax was added to the solution.