1. Proteomic Analysis to Examine the Role of Matrix Proteins in a Gouty Tophus from a Patient with Recurrent Gout
- Author
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Yoshifuru Tamura, Katsunori Inazawa, Shunya Uchida, Shin Fujimori, Kiyoko Kaneko, Ken-ichi Mawatari, Makoto Yasuda, Hiro Iwamoto, Tomoko Fukuuchi, Noriko Yamaoka, Kazuya Nakagomi, and Yasukazu Yamada
- Subjects
Male ,Proteomics ,Gout ,Chemistry ,Tophus ,Proteins ,Hyperuricemia ,General Medicine ,Matrix (biology) ,medicine.disease ,Weight range ,Biochemistry ,Gouty tophus ,Recurrence ,Monosodium urate ,Genetics ,medicine ,Humans ,Molecular Medicine ,Aged - Abstract
To examine the role of matrix proteins in the formation of gouty tophus, we analyzed the crystalline components and matrix proteins in a gouty tophus from a patient with recurrent gout. Micro-area X-ray diffraction analysis and infrared spectroscopy indicated that the tophus was composed of monosodium urate monohydrate. Proteomic analysis identified 134 proteins from the tophus as matrix proteins. Many proteins relevant to inflammation and host defense were identified, and immunoglobulin was detected in all four extracted fractions (KCl, formic acid, guanidine-HCl, and ethylenediaminetetraacetic acid) and from many spots throughout a broad molecular weight range after electrophoresis. It is thought that the process of biological defense including the immunity has occurred in the gouty tophus.
- Published
- 2014