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13 results on '"Hiraoka, Haruka"'

3. The effect of γ phosphate modified deoxynucleotide substrates on PCR activity and fidelity

Author

Hashiya, Fumitaka, primary, Murase, Hirotaka, additional, Chandela, Akash, additional, Hiraoka, Haruka, additional, Inagaki, Masahito, additional, Nakashima, Yuko, additional, Abe, Naoko, additional, Nakamura, Mayu, additional, Terai, Goro, additional, Kimura, Yasuaki, additional, Ando, Kaori, additional, Oka, Natsuhisa, additional, Asai, Kiyoshi, additional, and Abe, Hiroshi, additional

Published
2023
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4. Topological capture of mRNA for silencing gene expression

Author

Lyu, Fangjie, primary, Tomita, Takashi, additional, Abe, Naoko, additional, Hiraoka, Haruka, additional, Hashiya, Fumitaka, additional, Nakashima, Yuko, additional, Kajihara, Shiryu, additional, Tomoike, Fumiaki, additional, Shu, Zhaoma, additional, Onizuka, Kazumitsu, additional, Kimura, Yasuaki, additional, and Abe, Hiroshi, additional

Published
2023
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5. Creation of single molecular conjugates of metal–organic cages and DNA

Author

Nakajo, Toshinobu, primary, Kusaka, Shinpei, additional, Hiraoka, Haruka, additional, Nomura, Kohei, additional, Matsubara, Noriaki, additional, Baba, Rintaro, additional, Yoshida, Yuki, additional, Nakamoto, Kosuke, additional, Honma, Masakazu, additional, Iguchi, Hiroaki, additional, Uchihashi, Takayuki, additional, Abe, Hiroshi, additional, and Matsuda, Ryotaro, additional

Published
2023
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7. Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures.

Author

Inagaki, Masahito, primary, Abe, Naoko, additional, Li, Zhenmin, additional, Nakashima, Yuko, additional, Acharyya, Susit, additional, Ogawa, Kazuya, additional, Kawaguchi, Daisuke, additional, Hiraoka, Haruka, additional, Banno, Ayaka, additional, Meng, Zheyu, additional, Tada, Mizuki, additional, Ishida, Tatsuma, additional, Lyu, Pingxue, additional, Kokubo, Kengo, additional, Murase, Hirotaka, additional, Hashiya, Fumitaka, additional, Kimura, Yasuaki, additional, Uchida, Satoshi, additional, and Abe, Hiroshi, additional

Published
2022
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9. 望ましい職業観を育てる中学校道徳科の授業開発 : 「個人性」の視点を育てる教材の検討

Author

Hiraoka, Haruka and Miyasato, Tomoe

Subjects
望ましい職業観, 特別の教科道徳, 教材, ComputingMilieux_THECOMPUTINGPROFESSION, 授業開発, Personal aspect of occupation, 個人性, Special subject morality, Teaching materials, ComputingMilieux_COMPUTERSANDEDUCATION, Lesson development, Desirable occupational values
Abstract

The purpose of this research is to inspect the effect of the teaching materials to foster viewpoint of "Personal aspect of occupation" in the moral department of the junior high school. These teaching materials describe people who self-actualize through occupation. To achieve this purpose, the author conducted a class to 2nd grade students by using an existing teaching material. The author conducted a survey to inspect the effect of this class before and after the class. The survey asked students their occupational values. There was no difference of students' descriptions between before and after the class. It made clear the need to develop teaching materials students can remark "Personal aspect of occupation". Based on this need, the author developed teaching materials by interviewing to two workers. The author conducted the class with this teaching materials. As a result of this survey, students' descriptions which are related to "Personal aspect of occupation" increased. According to this result, this class contributes to foster viewpoint of "Personal aspect of occupation".

Published
2020

10. Antisense Oligonucleotide Modified with Disulfide Units Induces Efficient Exon Skipping in mdx Myotubes through Enhanced Membrane Permeability and Nucleus Internalization

Author

Hiraoka, Haruka, primary, Shu, Zhaoma, additional, Tri Le, Bao, additional, Masuda, Keiko, additional, Nakamoto, Kosuke, additional, Fangjie, Lyu, additional, Abe, Naoko, additional, Hashiya, Fumitaka, additional, Kimura, Yasuaki, additional, Shimizu, Yoshihiro, additional, Veedu, Rakesh N., additional, and Abe, Hiroshi, additional

Published
2021
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13. Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments.

Author

Nomura K, Onda K, Murase H, Hashiya F, Ono Y, Terai G, Oka N, Asai K, Suzuki D, Takahashi N, Hiraoka H, Inagaki M, Kimura Y, Shimizu Y, Abe N, and Abe H

Abstract

We developed chemically modified PCR primers that allow the design of flexible sticky ends by introducing a photo-cleavable group at the phosphate moiety. Nucleic acid derivatives containing o -nitrobenzyl photo-cleavable groups with a tert -butyl group at the benzyl position were stable during strong base treatment for oligonucleotide synthesis and thermal cycling in PCR reactions. PCR using primers incorporating these nucleic acid derivatives confirmed that chain extension reactions completely stopped at position 1 before and after the site of the photo-cleavable group was introduced. DNA fragments of 2 and 3 kbp, with sticky ends of 50 bases, were successfully concatenated with a high yield of 77%. A plasmid was constructed using this method. Finally, we applied this approach to construct a 48.5 kbp lambda phage DNA, which is difficult to achieve using restriction enzyme-based methods. After 7 days, we were able to confirm the generation of DNA of the desired length. Although the efficiency is yet to be improved, the chemically modified PCR primer offers potential to complement enzymatic methods and serve as a DNA concatenation technique., Competing Interests: The authors declare that they have no conflicts of interest., (This journal is © The Royal Society of Chemistry.)

Published
2024
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