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17 results on '"Hindley, James W."'

2. Microfluidic technologies for lipid vesicle generation.

Author

Cheng, Yu, Hay, Callum D., Mahuttanatan, Suchaya M., Hindley, James W., Ces, Oscar, and Elani, Yuval

Subjects
SYNTHETIC biology, ORIGIN of life, DRUG carriers, BIOMATERIALS, INDUSTRIAL capacity, POLYMERSOMES, BIOLOGICALLY inspired computing, NANOMEDICINE
Abstract

Encapsulating biological and non-biological materials in lipid vesicles presents significant potential in both industrial and academic settings. When smaller than 100 nm, lipid vesicles and lipid nanoparticles are ideal vehicles for drug delivery, facilitating the delivery of payloads, improving pharmacokinetics, and reducing the off-target effects of therapeutics. When larger than 1 μm, vesicles are useful as model membranes for biophysical studies, as synthetic cell chassis, as bio-inspired supramolecular devices, and as the basis of protocells to explore the origin of life. As applications of lipid vesicles gain prominence in the fields of nanomedicine, biotechnology, and synthetic biology, there is a demand for advanced technologies for their controlled construction, with microfluidic methods at the forefront of these developments. Compared to conventional bulk methods, emerging microfluidic methods offer advantages such as precise size control, increased production throughput, high encapsulation efficiency, user-defined membrane properties (i.e., lipid composition, vesicular architecture, compartmentalisation, membrane asymmetry, etc.), and potential integration with lab-on-chip manipulation and analysis modules. We provide a review of microfluidic lipid vesicle generation technologies, focusing on recent advances and state-of-the-art techniques. Principal technologies are described, and key research milestones are highlighted. The advantages and limitations of each approach are evaluated, and challenges and opportunities for microfluidic engineering of lipid vesicles to underpin a new generation of therapeutics, vaccines, sensors, and bio-inspired technologies are presented. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Light-triggered enzymatic reactions in nested vesicle reactors

Author

Hindley, James W., Elani, Yuval, McGilvery, Catriona M., Ali, Simak, Bevan, Charlotte L., Law, Robert V., Ces, Oscar, and Engineering & Physical Science Research Council (EPSRC)

Subjects
RELEASE, Science & Technology, Ultraviolet Rays, Science, Hydrolysis, fungi, food and beverages, LIPOSOMES, beta-Galactosidase, Article, Multidisciplinary Sciences, POLYMERIZATION, SIZE, Biocatalysis, Science & Technology - Other Topics, lcsh:Q, lcsh:Science
Abstract

Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and in bottom-up synthetic biology as chassis for artificial cells. In both these areas the introduction of light-responsive modules affords increased functionality, for example, to initiate enzymatic reactions in the vesicle interior with spatiotemporal control. Here we report a system composed of nested vesicles where the inner compartments act as phototransducers, responding to ultraviolet irradiation through diacetylene polymerisation-induced pore formation to initiate enzymatic reactions. The controlled release and hydrolysis of a fluorogenic β-galactosidase substrate in the external compartment is demonstrated, where the rate of reaction can be modulated by varying ultraviolet exposure time. Such cell-like nested microreactor structures could be utilised in fields from biocatalysis through to drug delivery., Matryoshka doll-like, nested vesicles, each containing a different ingredient to a chemical reaction, can serve as microreactors. Here, the authors developed a system in which mixing of the ingredients can be induced by irradiation with ultraviolet light.

Published
2018

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