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All birds produce vocalizations as a form of tcommunication with other individuals. Different from songbirds, crowing is a singing vocalization produced by chickens that cannot be learned through imitation. Some genes are assumed to be responsible for this activity. The long-crowing chickens have a melodious and long sound, so they are categorized as singing chickens. They are part of the biodiversity in Indonesia, which has high economic and socio-cultural value. Reviews about long-crowing chickens, especially in Indonesia, are still very rare. This article aims to identify the uniqueness and the existence of long-crowing chickens, together with the conservation efforts needed to manage them. Information was collected from journal articles and other relevant documents. There are four local chickens in Indonesia classified as long-crowing chickens. They are developed in different areas of the community with different socio-cultural characteristics. The fundamental differences among the breeds that can be quantified are in crowing duration and the number of syllables. The government has acknowledged that long-crowing chickens are important genetic resources; however, the association and individual keepers or enthusiasts are vital actors in conservation efforts. The information about long-crowing chickens in Indonesia is incomplete. The research activities that need to be conducted include exploring the population number and distribution, as well as documentation of the local knowledge of chicken breeders and enthusiasts.

Objective: This study aimed to identify, discover and explore the characteristics of the mtDNA genomes of Cemani chicken (Gallus gallus). Methods: This study used gDNA of Cemani chicken isolated from liver tissue. mtDNA sequencing was performed using WGS mtDNA analysis with nanopore technology by Oxford Nanopore Technologies GridION. Bioinformatics and data analysis were then performed. Results: This study showed that the length of the mtDNA genome is 16,789 bp, consisting of two ribosomal RNA (12S rRNA, 16S rRNA), 22 transfer RNA genes (trnR, trnG, trnK, trnD, trnS, trnY, trnC, trnN, trnA, trnW, trnM, trnQ, trnl, trnL, trnV, trnF, trnP, trnT, trnE, trnL, trnS, trnH), 13 protein-coding genes (PCGs) (ND4l, ND3, COX3, ATP6, ATP8, COX2, COX1, ND2, ND1, CYTB, ND6, ND5, ND4), and a noncoding control region (Dloop). Furthermore, analysis showed there were polymorphic sites and amino acid alterations when mtDNA Cemani chicken was aligned with references from GenBank. Conclusion: Site (988T>*) in Dloop genes and (328A>G) in ND3 genes which alter glycine to stop codon, were specific markers found only in Cemani chicken. [ABSTRACT FROM AUTHOR]