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4. The complete DNA sequence of yeast chromosome III

Author

Oliver, S.G., van der Aart, Q.J.M., Agostoni-Carbone, M.L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J.P.G., Benit, P., Berben, G., Bergantino, E., Biteau, N., Bolle, P.A., Bolotin-Fukuhara, M., Brown, A., Brown, A.J.P., Buhler, J.M., Carcano, C., Carignani, G., Cederberg, H., Chanet, R., Contreras, R., Crouzet, M., Daignan-Fornier, B., Defoor, E., Delgado, M., Demolder, J., Doira, C., Dubois, E., Dujon, B., Dusterhoft, A., Erdmann, D., Esteban, M., Fabre, F., Fairhead, C., Faye, G., Feldmann, H., Fiers, W., Francingues-Gaillard, M.C., Franco, L., Frontali, L., Fukuhara, H., Fuller, L.J., Galland, P., Gent, M.E., Gigot, D., Gilliquet, V., Glansdorff, N., Goffeau, A., Grenson, M., Grisanti, P., Grivell, L.A., de Haan, M., Haasemann, M., Hatat, D., Hoenicka, J., Hegemann, J., Herbert, C.J., Hilger, F., Hohmann, S., Hollenberg, C.P., Huse, K., Iborra, F., Indge, K.J., Isono, K., Jacq, C., Jacquet, M., James, C.M., Jauniaux, J.C., Jia, Y., Jimenez, A., Kelly, A., Kleinhans, U., Kreisl, P., Lanfranchi, G., Lewis, C., van der Linden, C.G., Lucchini, G., Lutzenkirchen, K., Maat, M.J., Mallet, L., Mannhaupt, G., Martegani, E., Mathieu, A., Maurer, C.T.C., McConnell, D., McKee, R.A., Messenguy, F., Mewes, H.W., Molemans, F., Montague, M.A., Muzi Falconi, M., Navas, L., Newlon, C.S., Noone, D., Pallier, C., Panzeri, L., Pearson, B.M., Perea, J., Philippsen, P., Pierard, A., Planta, R.J., Plevani, P., Poetsch, B., Pohl, F., Purnelle, B., Ramezani Rad, M., Rasmussen, S.W., Raynal, A., Remacha, M., Richterich, P., Roberts, A.B., Rodriguez, F., Sanz, E., Schaaff-Gerstenschlager, I., Scherens, B., Schweitzer, B., Shu, Y., Skala, J., Slonimski, P.P., Sor, F., Soustelle, C., Spiegelberg, R., Stateva, L.I., Steensma, H.Y., Steiner, S., Thierry, A., Thireos, G., Tzermia, M., Urrestarazu, L.A., Valle, G., Vetter, I., van Vliet-Reedijk, J.C., Voet, M., Volckaert, G., Vreken, P., Wang, H., Warmington, J.R., von Wettstein, D., Wicksteed, B.L., Wilson, C., Wurst, H., Xu, G., Yoshikawa, A., Zimmermann, F.K., and Sgouros, J.G.

Subjects
Saccharomyces -- Genetic aspects, Nucleotide sequence -- Research, Plant chromosomes -- Research, Genomes -- Research, Environmental issues, Science and technology, Zoology and wildlife conservation
Published
1992

6. The Yeast Genome Directory

Author

Goffeau, A., Aert, R., Agostini-Carbone, M. L., Ahmed, A., Aigle, M., Alberghina, L., Albermann, K., Albers, M., Aldea, M., Alexandraki, D., Aljinovic, G., Allen, E., Alt-Mörbe, J., André, B., Andrews, S., Ansorge, W., Antoine, G., Anwar, R., Aparicio, A., Araujo, R., Arino, J., Arnold, F., Arroyo, J., Aviles, E., Backes, U., Baclet, M. C., Badcock, K., Bahr, A., Baladron, V., Ballesta, J. P. G., Bankier, A. T., Banrevi, A., Bargues, M., Baron, L., Barreiros, T., Barrell, B. G., Barthe, C., Barton, A. B., Baur, A., Bécam, A.-M., Becker, A., Becker, I., Beinhauer, J., Benes, V., Benit, P., Berben, G., Bergantino, E., Bergez, P., Berno, A., Bertani, I., Biteau, N., Bjourson, A. J., Blöcker, H., Blugeon, C., Bohn, C., Boles, E., Bolle, P. A., Bolotin-Fukuhara, M., Bordonné, R., Boskovic, J., Bossier, P., Botstein, D., Bou, G., Bowman, S., Boyer, J., Brandt, P., Brandt, T., Brendel, M., Brennan, T., Brinkman, R., Brown, A., Brown, A. J. P., Brown, D., Brückner, M., Bruschi, C. V., Buhler, J. M., Buitrago, M. J., Bussereau, F., Bussey, H., Camasses, A., Carcano, C., Carignani, G., Carpenter, J., Casamayor, A., Casas, C., Castagnoli, L., Cederberg, H., Cerdan, E., Chalwatzis, N., Chanet, R., Chen, E., Chéret, G., Cherry, J. M., Chillingworth, T., Christiansen, C., Chuat, J.-C., Chung, E., Churcher, C., Churcher, C. M., Clark, M. W., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Colleaux, L., Connor, R., Contreras, R., Cooper, J., Copsey, T., Coster, F., Coster, R., Couch, J., Crouzet, M., Cziepluch, C., Daignan-Fornier, B., Dal Paro, F., Dang, D. V., D’Angelo, M., Davies, C. J., Davis, K., Davis, R. W., De Antoni, A., Dear, S., Dedman, K., Defoor, E., De Haan, M., Delaveau, Th., Del Bino, S., Delgado, M., Delius, H., Delneri, D., Del Rey, F., Demolder, J., Démolis, N., Devlin, K., de Wergifosse, P., Dietrich, F. S., Ding, H., Dion, C., Dipaolo, T., Doignon, F., Doira, C., Domdey, H., Dover, J., Du, Z., Dubois, E., Dujon, B., Duncan, M., Durand, P., Düsterhöft, A., Düsterhus, S., Eki, T., El Bakkoury, M., Eide, L. G., Entian, K.-D., Eraso, P., Erdmann, D., Erfle, H., Escribano, V., Esteban, M., Fabiani, L., Fabre, F., Fairhead, C., Fartmann, B., Favello, A., Faye, G., Feldmann, H., Fernandes, L., Feroli, F., Feuermann, M., Fiedler, T., Fiers, W., Fleig, U. N., Flöth, M., Fobo, G. M., Fortin, N., Foury, F., Francingues-Gaillard, M. C., Franco, L., Fraser, A., Friesen, J.D., Fritz, C., Frontali, L., Fukuhara, H., Fulton, L., Fuller, L. J., Gabel, C., Gaillardin, C., Gaillon, L., Galibert, F., Galisson, F., Galland, P., Gamo, F.-J., Gancedo, C., Garcia-Cantalejo, J. M., García-Gonzalez, M. I., Garcia-Ramirez, J. J., García-Saéz, M., Gassenhuber, H., Gatius, M., Gattung, S., Geisel, C., Gent, M. E., Gentles, S., Ghazvini, M., Gigot, D., Gilliquet, V., Glansdorff, N., Gómez-Peris, A., Gonzaléz, A., Goulding, S. E., Granotier, C., Greco, T., Grenson, M., Grisanti, P., Grivell, L. A., Grothues, D., Gueldener, U., Guerreiro, P., Guzman, E., Haasemann, M., Habbig, B., Hagiwara, H., Hall, J., Hallsworth, K., Hamlin, N., Hand, N. J., Hanemann, V., Hani, J., Hankeln, T., Hansen, M., Harris, D., Harris, D. E., Hartzell, G., Hatat, D., Hattenhorst, U., Hawkins, J., Hebling, U., Hegemann, J., Hein, C., Hennemann, A., Hennessy, K., Herbert, C. J., Hernandez, K., Hernando, Y., Herrero, E., Heumann, K., Heuss- Neitzel, D., Hewitt, N., Hiesel, R., Hilbert, H., Hilger, F., Hillier, L., Ho, C., Hoenicka, J., Hofmann, B., Hoheisel, J., Hohmann, S., Hollenberg, C. P., Holmstrøm, K., Horaitis, O., Horsnell, T. S., Huang, M.-E., Hughes, B., Hunicke-Smith, S., Hunt, S., Hunt, S. E., Huse, K., Hyman, R. W., Iborra, F., Indge, K. J., Iraqui Houssaini, I., Isono, K., Jacq, C., Jacquet, M., Jacquier, A., Jagels, K., Jäger, W., James, C. M., Jauniaux, J. C., Jia, Y., Jier, M., Jimenez, A., Johnson, D., Johnston, L., Johnston, M., Jones, M., Jonniaux, J.-L., Kaback, D. B., Kallesøe, T., Kalman, S., Kalogeropoulos, A., Karpfinger-Hartl, L., Kashkari, D., Katsoulou, C., Kayser, A., Kelly, A., Keng, T., Keuchel, H., Kiesau, P., Kirchrath, L., Kirsten, J., Kleine, K., Kleinhans, U., Klima, R., Komp, C., Kordes, E., Korol, S., Kötter, P., Krämer, C., Kramer, B., Kreisl, P., Kucaba, T., Kuester, H., Kurdi, O., Laamanen, P., Lafuente, M. J., Landt, O., Lanfranchi, G., Langston, Y., Lashkari, D., Latreille, P., Lauquin, G., Le, T., Legrain, P., Legros, Y., Lepingle, A., Lesveque, H., Leuther, H., Lew, H., Lewis, C., Li, Z. Y., Liebl, S., Lin, A., Lin, D., Logghe, M., Lohan, A. J. E., Louis, E. J., Lucchini, G., Lutzenkirchen, K., Lyck, R., Lye, G., Maarse, A. C., Maat, M. J., Macri, C., Madania, A., Maftahi, M., Maia e Silva, A., Maillier, E., Mallet, L., Mannhaupt, G., Manus, V., Marathe, R., Marck, C., Marconi, A., Mardis, E., Martegani, E., Martin, R., Mathieu, A., Maurer, C. T. C., Mazón, M. J., Mazzoni, C., McConnell, D., McDonald, S., McKee, R. A., McReynolds, A. D. K., Melchioretto, P., Menezes, S., Messenguy, F., Mewes, H. W., Michaux, G., Miller, N., Minenkova, O., Miosga, T., Mirtipati, S., Möller-Rieker, S., Möstl, D., Molemans, F., Monnet, A., Monnier, A-L., Montague, M. A., Moro, M., Mosedale, D., Möstl, D., Moule, S., Mouser, L., Murakami, Y., Müller-Auer, S., Mulligan, J., Murphy, L., Muzi Falconi, M., Naitou, M., Nakahara, K., Namath, A., Nasr, F., Navas, L., Nawrocki, A., Nelson, J., Nentwich, U., Netter, P., Neu, R., Newlon, C. S., Nhan, M., Nicaud, J.-M., Niedenthal, R. K., Nombela, C., Noone, D., Norgren, R., Nußbaumer, B., Obermaier, B., Odell, C., Öfner, P., Oh, C., Oliver, K., Oliver, S. G., Ouellette, B. F., Ozawa, M., Paces, V., Pallier, C., Pandolfo, D., Panzeri, L., Paoluzi, S., Parle-Mcdermott, A. G., Pascolo, S., Patricio, N., Pauley, A., Paulin, L., Pearson, B. M., Pearson, D., Peluso, D., Perea, J., Pérez-Alonso, M., Pérez-Ortin, J. E., Perrin, A., Petel, F. X., Pettersson, B., Pfeiffer, F., Philippsen, P., Piérard, A., Piravandi, E., Planta, R. J., Plevani, P., Poch, O., Poetsch, B., Pohl, F. M., Pohl, T. M., Pöhlmann, R., Poirey, R., Portetelle, D., Portillo, F., Potier, S., Proft, M., Prydz, H., Pujol, A., Purnelle, B., Puzos, V., Rajandream, M. A., Ramezani Rad, M., Rasmussen, S. W., Raynal, A., Rechmann, S., Remacha, M., Revuelta, J. L., Rice, P., Richard, G-F., Richterich, P., Rieger, M., Rifken, L., Riles, L., Rinaldi, T., Rinke, M., Roberts, A. B., Roberts, D., Rodriguez, F., Rodriguez-Belmonte, E., Rodriguez-Pousada, C., Rodriguez-Torres, A. M., Rose, M., Rossau, R., Rowley, N., Rupp, T., Ruzzi, M., Saeger, W., Saiz, J. E., Saliola, M., Salom, D., Saluz, H. P., Sánchez-Perez, M., Santos, M. A., Sanz, E., Sanz, J. E., Saren, A.-M., Sartorello, F., Sasanuma, M., Sasanuma, S-I., Scarcez, T., Schaaf-Gerstenschläger, I., Schäfer, B., Schäfer, M., Scharfe, M., Scherens, B., Schroff, N., Sen-Gupta, M., Shibata, T., Schmidheini, T., Schmidt, E. R., Schneider, C., Scholler, P., Schramm, S., Schreer, A., Schröder, M., Schwager, C., Schwarz, S., Schwarzlose, C., Schweitzer, B., Schweizer, M., Sdicu, A-M., Sehl, P., Sensen, C., Sgouros, J. G., Shogren, T., Shore, L., Shu, Y., Skala, J., Skelton, J., Slonimski, P. P., Smit, P. H. M., Smith, V., Soares, H., Soeda, E., Soler-Mira, A., Sor, F., Soriano, N., Souciet, J. L., Soustelle, C., Spiegelberg, R., Stateva, L. I., Steensma, H. Y., Stegemann, J., Steiner, S., Stellyes, L., Sterky, F., Storms, R. K., St. Peter, H., Stucka, R., Taich, A., Talla, E., Tarassov, I., Tashiro, H., Taylor, P., Teodoru, C., Tettelin, H., Thierry, A., Thireos, G., Tobiasch, E., Tovan, D., Trevaskis, E., Tsuchiya, Y., Tzermia, M., Uhlen, M., Underwood, A., Unseld, M., Urbanus, J. H. M., Urrestarazu, A., Ushinsky, S., Valens, M., Valle, G., Van Broekhoven, A., Vandenbol, M., Van Der Aart, Q. J. M., Van Der Linden, C. G., Van Dyck, L., Vanoni, M., Van Vliet-Reedijk, J. C., Vassarotti, A., Vaudin, M., Vaughan, K., Verhasselt, P., Vetter, I., Vierendeels, F., Vignati, D., Vilela, C., Vissers, S., Vleck, C., Vo, D. T., Vo, D. H., Voet, M., Volckaert, G., Von Wettstein, D., Voss, H., Vreken, P., Wagner, G., Walsh, S. V., Wambutt, R., Wang, H., Wang, Y., Warmington, J. R., Waterston, R., Watson, M. D., Weber, N., Wedler, E., Wedler, H., Wei, Y., Whitehead, S., Wicksteed, B. L., Wiemann, S., Wilcox, L., Wilson, C., Wilson, R., Winant, A., Winnett, E., Winsor, B., Wipfli, P., Wölfl, S., Wohldman, P., Wolf, K., Wolfe, K. H., Wright, L. F., Wurst, H., Xu, G., Yamasaki, M., Yelton, M. A., Yokohama, K., Yoshikawa, A., Yuping, S., Zaccaria, P., Zagulski, M., Zimmermann, F. K., Zimmermann, J., Zimmermann, M., Zhong, W-W., Zollner, A., and Zumstein, E.

Published
1997
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11. The nucleotide sequence of Saccharomyces cerevisiae chromosome XII

Author

Mark Johnston, Hillier, L., Riles, L., Albermann, K., André, B., Ansorge, W., Benes, V., Brückner, M., Delius, H., Dubois, E., Düsterhöft, A., Entian, K. -D, Floeth, M., Goffeau, A., Hebling, U., Heumann, K., Heuss-Neitzel, D., Hilbert, H., Hilger, F., Kleine, K., Kötter, P., Louis, E. J., Messenguy, F., Mewes, H. W., Miosga, T., Möstl, D., Müller-Auer, S., Nentwich, U., Obermaier, B., Piravandi, E., Pohl, T. M., Portetelle, D., Purnelle, B., Rechmann, S., Rieger, M., Rinke, M., Rose, M., Scharfe, M., Scherens, B., Scholler, P., Schwager, C., Schwarz, S., Underwood, A. P., Urrestarazu, L. A., Vandenbol, M., Verhasselt, P., Vierendeels, F., Voet, M., Volckaert, G., Voss, H., Wambutt, R., Wedler, E., Wedler, H., Zimmermann, F. K., Zollner, A., Hani, J., and Hoheisel, J. D.

Subjects
Base Sequence, Molecular Sequence Data, Saccharomyces cerevisiae, Chromosomes, Fungal, DNA, Fungal, Article
Abstract

The yeast Saccharomyces cerevisiae is the pre-eminent organism for the study of basic functions of eukaryotic cells(1). All of the genes of this simple eukaryotic cell have recently been revealed by an international collaborative effort to determine the complete DNA sequence of its nuclear genome. Here we describe some of the features of chromosome XII.

Published
1997

12. The complete sequence of the yeast chromosome III

Author

Oliver, S. G., van der Aart, Q. J. M., Agostoni Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P. G., Benit, P., Berben, G., Bergantino, Elisabetta, Biteau, N., Bolle, P. A., Bolotin Fukuhara, M., Brown, A., Brown, A. J. P., Buhler, J. M., Carcano, C., Carignani, G., Cederberg, H., Chanet, R., Contreras, R., Crouzet, M., Daignan Fornier, B., Defoor, E., Delgado, M., Demolder, J., Doira, C., Dubois, E., Dujon, B., Dusterhoft, A., Erdmann, D., Esteban, M., Fabre, F., Fairhead, C., Faye, G., Feldmann, H., Fiers, W., Francingues Gaillard, M. C., Franco, L., Frontali, L., Fukuhara, H., Fuller, L. J., Galland, P., Gent, M. E., Gigot, D., Gilliquet, V., Glansdorff, N., Goffeau, A., Grenson, M., Grisanti, P., Grivell, L. A., de Haan, M., Haasemann, M., Hatat, D., Hoenicka, J., Hegemann, J., Herbert, C. J., Hilger, F., Hohmann, S., Hollenberg, C. P., Huse, K., Iborra, F., Indje, K. J., Isono, K., Jacq, C., Jacquet, M., James, C. M., Jauniaux, J. C., Jia, Y., Jimenez, A., Kelly, A., Kleinhans, U., Kreisl, P., Lanfranchi, Gerolamo, Lewis, C, Vanderlinden, C. G., Lucchini, G., Lutzenkirchen, K, Maat, M. J., Mallet, L., Mannhaupet, G., Martegani, E., Mathieu, A., Maurer, C. T. C., Mcconnell, D., Mckee, R. A., Messenguy, F., Mewes, H. W., Molemans, F., Montague, M. A., Muzi Falconi, M., Navas, L., Newlon, C. S., Noone, D., Pallier, C., Panzeri, L., Pearson, B. M., Perea, J., Philippsen, P., Pierard, A., Planta, R. J., Plevani, P., Poetsch, B., Pohl, F., Purnelle, B., Ramezani Rad, M., Rasmussen, S. W., Raynal, A., Remacha, M., Richterich, P., Roberts, A. B., Rodriguez, F., Sanz, E., Schaaff Gerstenschlager, I., Scherens, B., Schweitzer, B., Shu, Y., Skala, J., Slonimski, P. P., Sor, F., Soustelle, C., Spiegelberg, R., Stateva, L. I., Steensma, S., Steiner, H. Y., Thierry, A., Thireos, G., Tzermia, M., Urrestarazu, L. A., Valle, Giorgio, Vetter, I., van Vliet Reedijk, J. C., Voet, M., Volckaert, G., Vreken, P., Wang, H., Warmington, J. R., von Wettstein, D., Wicksteed, B. L., Wilson, C., Wurst, H., Xu, G., Yoshikawa, A., Zimmermann, F. K., and Sgouros, J. G.

Published
1992

14. The nucleotide sequence of Saccharomyces cerevisiae chromosome XV

Author

Dujon, B, Albermann, K, Aldea, M, Alexandraki, D, Ansorge, W, Arino, J, Benes, V, Bohn, C, BolotinFukuhara, M, Bordonne, R, Boyer, J, Camasses, A, Casamayor, A, Casas, C, Cheret, G, Cziepluch, C, DaignanFornier, B, Dang, V, deHaan, M, Delius, H, Durand, P, Fairhead, C, Feldmann, H, Gaillon, L, Galisson, F, Gamo, J, Gancedo, C, Goffeau, A, Goulding, E, Grivell, A, Habbig, B, Hand, J, Hani, J, Hattenhorst, U, Hebling, U, Hernando, Y, Herrero, E, Heumann, K, Hiesel, R, Hilger, F, Hofmann, B, Hollenberg, P, Hughes, B, Jauniaux, C, Kalogeropoulos, A, Katsoulou, C, Kordes, E, Lafuente, J, Landt, O, Louis, J, Maarse, C, Madania, A, Mannhaupt, G, Marck, C, Martin, P, Mewes, W, Michaux, G, Paces, V, ParleMcDermott, G, Pearson, M, Perrin, A, Pettersson, B, Poch, O, Pohl, M, Poirey, R, Portetelle, D, Pujol, A, Purnelle, B, Rad, R, Rechmann, S, Schwager, C, Schweizer, M, Sor, F, Sterky, Fredrik, Tarassov, A, Teodoru, C, Tettelin, H, Thierry, A, Tobiasch, E, Tzermia, M, Uhlen, Mathias, Unseld, M, Valens, M, Vandenbol, M, Vetter, I, Vicek, C, Voet, M, Volckaert, G, Voss, H, Wambutt, R, Wedler, H, Wiemann, S, Winsor, B, Wolfe, H, Zollner, A, Zumstein, E, Kleine, K, Dujon, B, Albermann, K, Aldea, M, Alexandraki, D, Ansorge, W, Arino, J, Benes, V, Bohn, C, BolotinFukuhara, M, Bordonne, R, Boyer, J, Camasses, A, Casamayor, A, Casas, C, Cheret, G, Cziepluch, C, DaignanFornier, B, Dang, V, deHaan, M, Delius, H, Durand, P, Fairhead, C, Feldmann, H, Gaillon, L, Galisson, F, Gamo, J, Gancedo, C, Goffeau, A, Goulding, E, Grivell, A, Habbig, B, Hand, J, Hani, J, Hattenhorst, U, Hebling, U, Hernando, Y, Herrero, E, Heumann, K, Hiesel, R, Hilger, F, Hofmann, B, Hollenberg, P, Hughes, B, Jauniaux, C, Kalogeropoulos, A, Katsoulou, C, Kordes, E, Lafuente, J, Landt, O, Louis, J, Maarse, C, Madania, A, Mannhaupt, G, Marck, C, Martin, P, Mewes, W, Michaux, G, Paces, V, ParleMcDermott, G, Pearson, M, Perrin, A, Pettersson, B, Poch, O, Pohl, M, Poirey, R, Portetelle, D, Pujol, A, Purnelle, B, Rad, R, Rechmann, S, Schwager, C, Schweizer, M, Sor, F, Sterky, Fredrik, Tarassov, A, Teodoru, C, Tettelin, H, Thierry, A, Tobiasch, E, Tzermia, M, Uhlen, Mathias, Unseld, M, Valens, M, Vandenbol, M, Vetter, I, Vicek, C, Voet, M, Volckaert, G, Voss, H, Wambutt, R, Wedler, H, Wiemann, S, Winsor, B, Wolfe, H, Zollner, A, Zumstein, E, and Kleine, K

Abstract

Chromosome XV was one of the last two chromosomes of Saccharomyces cerevisiae to be discovered(1). It is the third-largest yeast chromosome after chromosomes XII and IV, and is very similar in size to chromosome VII. It alone represents 9% of the yeast genome (8% if ribosomal DNA is included). When systematic sequencing of chromosome XV was started, 93 genes or markers were identified, and most of them were mapped(2). However, very little else was known about chromosome XV which, in contrast to shorter chromosomes, had not been the object of comprehensive genetic or molecular analysis. It was therefore decided to start sequencing chromosome XV only in the third phase of the European Yeast Genome Sequencing Programme, after experience was gained on chromosomes III, XI and II (refs 3-5). The sequence of chromosome XV has been determined from a set of partly overlapping cosmid clones derived from a unique yeast strain, and physically mapped at 3.3-kilobase resolution before sequencing. As well as numerous new open reading frames (ORFs) and genes encoding tRNA or small RNA molecules, the sequence of 1,091,283 base pairs confirms the high proportion of orphan genes and reveals a number of ancestral and successive duplications with other yeast chromosomes., QC 20100812

Published
1997
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15. The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.

Author

Johnston, Mark, Hillier, L, Riles, Linda, Albermann, K, André, Bruno, Ansorge, W., Benes, V., Bruckner, M., Delius, Heike, Dubois, Evelyne, Düsterhöft, A, Entian, Karl-Dieter, Floeth, M, Goffeau, A, Hebling, U, Heumann, K, Heuss-Neitzel, D, Hilbert, H, Hilger, F, Kleine, K, Kötter, Peter, Louis, Edouard, Messenguy, Francine, Mewes, H W, Hoheisel, J D, Johnston, Mark, Hillier, L, Riles, Linda, Albermann, K, André, Bruno, Ansorge, W., Benes, V., Bruckner, M., Delius, Heike, Dubois, Evelyne, Düsterhöft, A, Entian, Karl-Dieter, Floeth, M, Goffeau, A, Hebling, U, Heumann, K, Heuss-Neitzel, D, Hilbert, H, Hilger, F, Kleine, K, Kötter, Peter, Louis, Edouard, Messenguy, Francine, Mewes, H W, and Hoheisel, J D

Abstract

The yeast Saccharomyces cerevisiae is the pre-eminent organism for the study of basic functions of eukaryotic cells. All of the genes of this simple eukaryotic cell have recently been revealed by an international collaborative effort to determine the complete DNA sequence of its nuclear genome. Here we describe some of the features of chromosome XII., Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
1997

17. The nucleotide sequence of Saccharomyces cerevisiae chromosome XV

Author

Dujon, B., primary, Albermann, K., additional, Aldea, M., additional, Alexandraki, D., additional, Ansorge, W., additional, Arino, J., additional, Benes, V., additional, Bohn, C., additional, Bolotin-Fukuhara, M., additional, Bordonné, R., additional, Boyer, J., additional, Camasses, A., additional, Casamayor, A., additional, Casas, C., additional, Chéret, G., additional, Cziepluch, C., additional, Daignan-Fornier, B., additional, Dang, D. V., additional, de Haan, M., additional, Delius, H., additional, Durand, P., additional, Fairhead, C., additional, Feldmann, H., additional, Gaillon, L., additional, Galisson, F., additional, Gamo, F.-J., additional, Gancedo, C., additional, Goffeau, A., additional, Goulding, S. E., additional, Grivell, L. A., additional, Habbig, B., additional, Hand, N. J., additional, Hani, J., additional, Hattenhorst, U., additional, Hebling, U., additional, Hernando, Y., additional, Herrero, E., additional, Heumann, K., additional, Hiesel, R., additional, Hilger, F., additional, Hofmann, B., additional, Hollenberg, C. P., additional, Hughes, B., additional, Jauniaux, J.-C., additional, Kalogeropoulos, A., additional, Katsoulou, C., additional, Kordes, E., additional, Lafuente, M. J., additional, Landt, O., additional, Louis, E. J., additional, Maarse, A. C., additional, Madania, A., additional, Mannhaupt, G., additional, Marck, C., additional, Martin, R. P., additional, Mewes, H. W., additional, Michaux, G., additional, Paces, V., additional, Parle-McDermott, A. G., additional, Pearson, B. M., additional, Perrin, A., additional, Pettersson, B., additional, Poch, O., additional, Pohl, T. M., additional, Poirey, R., additional, Portetelle, D., additional, Pujol, A., additional, Purnelle, B., additional, Ramezani Rad, M., additional, Rechmann, S., additional, Schwager, C., additional, Schweizer, M., additional, Sor, F., additional, Sterky, F., additional, Tarassov, I. A., additional, Teodoru, C., additional, Tettelin, H., additional, Thierry, A., additional, Tobiasch, E., additional, Tzermia, M., additional, Uhlen, M., additional, Unseld, M., additional, Valens, M., additional, Vandenbol, M., additional, Vetter, I., additional, Vlcek, C., additional, Voet, M., additional, Volckaert, G., additional, Voss, H., additional, Wambutt, R., additional, Wedler, H., additional, Wiemann, S., additional, Winsor, B., additional, Wolfe, K. H., additional, Zollner, A., additional, Zumstein, E., additional, and Kleine, K., additional

Published
1997
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18. The nucleotide sequence of Saccharomyces cerevisiae chromosome VII

Author

Tettelin, H., primary, Agostoni Carbone, M. L., additional, Albermann, K., additional, Albers, M., additional, Arroyo, J., additional, Backes, U., additional, Barreiros, T., additional, Bertani, I., additional, Bjourson, A. J., additional, Brückner, M., additional, Bruschi, C. V., additional, Carignani, G., additional, Castagnoli, L., additional, Cerdan, E., additional, Clemente, M. L., additional, Coblenz, A., additional, Coglievina, M., additional, Coissac, E., additional, Defoor, E., additional, Del Bino, S., additional, Delius, H., additional, Delneri, D., additional, de Wergifosse, P., additional, Dujon, B., additional, Durand, P., additional, Entian, K. D., additional, Eraso, P., additional, Escribano, V., additional, Fabiani, L., additional, Fartmann, B., additional, Feroli, F., additional, Feuermann, M., additional, Frontali, L., additional, García-Gonzalez, M., additional, García-Sáez, M. I., additional, Goffeau, A., additional, Guerreiro, P., additional, Hani, J., additional, Hansen, M., additional, Hebling, U., additional, Hernandez, K., additional, Heumann, K., additional, Hilger, F., additional, Hofmann, B., additional, Indge, K. J., additional, James, C. M., additional, Klima, R., additional, Kötter, P., additional, Kramer, B., additional, Kramer, W., additional, Lauquin, G., additional, Leuther, H., additional, Louis, E. J., additional, Maillier, E., additional, Marconi, A., additional, Martegani, E., additional, Mazón, M. J., additional, Mazzoni, C., additional, McReynolds, A. D. K., additional, Melchioretto, P., additional, Mewes, H. W., additional, Minenkova, O., additional, Müller-Auer, S., additional, Nawrocki, A., additional, Netter, P., additional, Neu, R., additional, Nombela, C., additional, Oliver, S. G., additional, Panzeri, L., additional, Paoluzi, S., additional, Plevani, P., additional, Portetelle, D., additional, Portillo, F., additional, Potier, S., additional, Purnelle, B., additional, Rieger, M., additional, Riles, L., additional, Rinaldi, T., additional, Robben, J., additional, Rodrigues-Pousada, C., additional, Rodriguez-Belmonte, E., additional, Rodriguez-Torres, A. M., additional, Rose, M., additional, Ruzzi, M., additional, Saliola, M., additional, Sánchez-Perez, M., additional, Schäfer, B., additional, Schäfer, M., additional, Scharfe, M., additional, Schmidheini, T., additional, Schreer, A., additional, Skala, J., additional, Souciet, J. L., additional, Steensma, H. Y., additional, Talla, E., additional, Thierry, A., additional, Vandenbol, M., additional, van der Aart, Q. J. M., additional, Van Dyck, L., additional, Vanoni, M., additional, Verhasselt, P., additional, Voet, M., additional, Volckaert, G., additional, Wambutt, R., additional, Watson, M. D., additional, Weber, N., additional, Wedler, E., additional, Wedler, H., additional, Wipfli, P., additional, Wolf, K., additional, Wright, L. F., additional, Zaccaria, P., additional, Zimmermann, M., additional, Zollner, A., additional, and Kleine, K., additional

Published
1997
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19. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.

Author

Galibert, F., primary, Alexandraki, D., additional, Baur, A., additional, Boles, E., additional, Chalwatzis, N., additional, Chuat, J. C., additional, Coster, F., additional, Cziepluch, C., additional, De Haan, M., additional, Domdey, H., additional, Durand, P., additional, Entian, K. D., additional, Gatius, M., additional, Goffeau, A., additional, Grivell, L. A., additional, Hennemann, A., additional, Herbert, C. J., additional, Heumann, K., additional, Hilger, F., additional, Hollenberg, C. P., additional, Huang, M. E., additional, Jacq, C., additional, Jauniaux, J. C., additional, Katsoulou, C., additional, and Karpfinger-Hartl, L., additional

Published
1996
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20. Complete DNA sequence of yeast chromosome XI

Author

Dujon, B., primary, Alexandraki, D., additional, André, B., additional, Ansorge, W., additional, Baladron, V., additional, Ballesta, J. P. G., additional, Banrevi, A., additional, Bolle, P. A., additional, Bolotin-Fukuhara, M., additional, Bossier, P., additional, Bou, G., additional, Boyer, J., additional, Buitrago, M. J., additional, Cherét, G., additional, Colleaux, L., additional, Dalgnan-Fornier, B., additional, del Rey, F., additional, Dion, C., additional, Domdey, H., additional, Düsterhöft, A., additional, Düsterhus, S., additional, Entian, K.-D., additional, Erfle, H., additional, Esteban, P. F., additional, Feldmann, H., additional, Fernandes, L., additional, Fobo, G. M., additional, Fritz, C., additional, Fukuhara, H., additional, Gabel, C., additional, Gaillon, L., additional, Carcia-Cantalejo, J. M., additional, Garcia-Ramirez, J. J., additional, Gent, M. E., additional, Ghazvini, M., additional, Goffeau, A., additional, Gonzaléz, A., additional, Grothues, D., additional, Guerreiro, P., additional, Hegemann, J., additional, Hewitt, N., additional, Hilger, F., additional, Hollenberg, C. P., additional, Horaitis, O., additional, Indge, K. J., additional, Jacquier, A., additional, James, C. M., additional, Jauniaux, J. C., additional, Jimenez, A., additional, Keuchel, H., additional, Kirchrath, L., additional, Kleine, K., additional, Kötter, P., additional, Legrain, P., additional, Liebl, S., additional, Louis, E. J., additional, Maia e Silva, A., additional, Marck, C., additional, Monnier, A.-L., additional, Möstl, D., additional, Müller, S., additional, Obermaier, B., additional, Oliver, S. G., additional, Pallier, C., additional, Pascolo, S., additional, Pfeiffer, F., additional, Philippsen, P., additional, Planta, R. J., additional, Pohl, F. M., additional, Pohl, T. M., additional, Pöhlmann, R., additional, Portetelle, D., additional, Purnelle, B., additional, Puzos, V., additional, Rad, M. Ramezani, additional, Rasmussen, S. W., additional, Remacha, M., additional, Revuelta, J. L., additional, Richard, G.-F., additional, Rieger, M., additional, Rodrigues-Pousada, C., additional, Rose, M., additional, Rupp, T., additional, Santos, M. A., additional, Schwager, C., additional, Sensen, C., additional, Skala, J., additional, Soares, H., additional, Sor, F., additional, Stegemann, J., additional, Tettelin, H., additional, Thierry, A., additional, Tzermia, M., additional, Urrestarazu, L. A., additional, van Dyck, L., additional, van Vliet-Reedijk, J. C., additional, Valens, M., additional, Vandenbo, M., additional, Vilela, C., additional, Vissers, S., additional, von Wettstein, D., additional, Voss, H., additional, Wiemann, S., additional, Xu, G., additional, Zimmermann, J., additional, Haasemann, M., additional, Becker, I., additional, and Mewes, H. W., additional

Published
1994
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25. Yeast cdc35 mutants are defective in adenylate cyclase and are allelic with cyr1 mutants while CAS1, a new gene, is involved in the regulation of adenylate cyclase.

Author

Boutelet, F., Petitjean, A., and Hilger, F.

Abstract

Newly isolated temperature‐sensitive cdc35 mutants of Saccharomyces cerevisiae have been characterized. They show the morphology, growth and conjugation characteristics typical of class‐A or class‐II start mutants. The cdc35 mutation induces a significant decrease of the intracellular cAMP level and produces a thermolabile adenylate cyclase. By classical genetic criteria the CDC35 gene is identical with the structural gene of adenylate cyclase, CYR1. The results of the mutant selection, the kinetics of macromolecule accumulation and the cell‐density change of cdc35 mutants at the restrictive temperature, indicate that CDC35 function may not be cell cycle‐specific. A new mutation, cas1, was isolated and partially characterized. It mediates the suppression by external cAMP of the unlinked cdc35 mutation. It causes a slight increase of the intracellular cAMP level and has strong effects on the adenylate cyclase activities, especially on the Mg2+ dependent activity. The data suggest that the CAS1 protein is a controlling element of adenylated cyclase. The CAS1 locus is different from the RAS1 and RAS2 loci.

Published
1985
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26. Evidence for a new chromosome in Saccharomyces cerevisiae

Author

Wickner, R B, Boutelet, F, and Hilger, F

Abstract

The current yeast map has 16 chromosomes, each originally defined by a centromere-linked gene unlinked to previously defined centromere markers. We examined four genes, cly2, KRB1, AMY2, and tsm0115, each centromere linked, but previously thought to be not on chromosomes I to XVI. We found that AMY2 is linked to cly2, and both are on chromosome II. tsm0115 is on the left arm of chromosome XVI. We confirm the earlier evidence that KRB1 is not on chromosomes I through XVI. This gene thus defines a new chromosome XVII. We also report meiotic linkage of met4 and pet8 (on chromosome XIV), confirming the connection between the petx-kex2 fragment of XIV and the centromere of XIV.

Published
1983
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27. Genetic mapping of arg1 and arg8 in Saccharomyces cerevisiae by trisomic analysis combined with interallelic complementation

Author

Hilger, F and Mortimer, R K

Abstract

Through use of multiply disomic strains, the genes arg1 and arg8 were excluded from all of chromosomes I to XVII except (i) XV and (ii) IX and XV, respectively. Further aneuploid analyses showed that these two genes were on the same chromosome. By tetrad analysis, arg1 was shown to be linked to SUP3 on the left arm of chromosome XV (parental ditype:nonparental ditype:tetratype = 74; 6:139) and arg8 was shown to be loosely linked to arg1 (parental ditype:nonparental ditype:tetratype 72:17:220) on the same arm. The sequence of the genes on this chromosome arm is centromere-SUP3-arg8. Because arg1 had previously been used to define an 18th chromosome, these results reestablished the minimum chromosome number in Saccharomyces cerevisiae as 17.

Published
1980
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28. Thermosensitive mutations affecting ribonucleic acid polymerases in Saccharomyces cerevisiae

Author

Thonart, P, Bechet, J, Hilger, F, and Burny, A

Abstract

Among 150 temperature-sensitive Saccharomyces cerevisiae mutants which we have isolated, 15 are specifically affected in ribonucleic acid (RNA) synthesis. Four of these mutants exhibit particularly drastic changes and were chosen for a more detailed study. In these four mutants, RNA synthesis is immediately blocked after a shift at the nonpermissive temperature (37 C), protein synthesis decays at a rate compatible with messenger RNA half-life, and deoxyribonucleic acid synthesis increases by about 40%. All the mutations display a recessive phenotype. The segregation of the four allelic pairs ts-/ts+ in diploids is mendelian, and the four mutants belong to three complementation groups. The elution patterns (diethylaminoethyl-Sephadex) of the three RNA polymerases of the mutants grown at 37 C for 3.5 h show very low residual activities. The in vitro thermodenaturation confirms the in vivo results; the half-lives of the mutant activities at 45 C are 10 times smaller than those of the wild-type enzymes. Polyacrylamide gel electrophoresis shows that the synthesis of all species of RNA is thermosensitive. The existence of three distinct genes, which are each indispensable for the activity of the three RNA polymerases in vivo as well as in vitro, strongly favors the hypothesis of three common subunits in the three RNA polymerases.

Published
1976
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29. Cyclic AMP controls the plasma membrane H+-ATPase activity from Saccharomyces cerevisiae.

Author

UCL, Ulaszewski, S., Hilger, F, Goffeau, André, UCL, Ulaszewski, S., Hilger, F, and Goffeau, André

Abstract

The thermosensitive G1-arrested cdc35-10 mutant from Saccharomyces cerevisiae, defective in adenylate cyclase activity, was shifted to restrictive temperature. After 1 h incubation at this temperature, the plasma membrane H+-ATPase activity of cdc35-10 was reduced to 50%, whereas that in mitochondria doubled. Similar data were obtained with cdc25, another thermosensitive G1-arrested mutant modified in the cAMP pathway. In contrast, the ATPase activities of the G1-arrested mutant cdc19, defective in pyruvate kinase, were not affected after 2 h incubation at restrictive temperature. In the double mutants cdc35-10 cas1 and cdc25 cas1, addition of extracellular cAMP prevented the modifications of ATPase activities observed in the single mutants cdc35-10 and cdc25. These data indicate that cAMP acts as a positive effector on the H+-ATPase activity of plasma membranes and as a negative effector on that of mitochondria.

Published
1989

30. Le Facteur de Santa Cruz : Fox / F. Barcellini. Pour un dollar : Fox / G. Magenta. Hop digui-di / A. Hoffman ; D. Manning. ICH SPIEL' KLAVIER MIT EINEM FINGER : Fox / D. Hilger ; Onésime Grosbois et son piano d'occasion

Author

Manning, Dick. Auteur ou responsable intellectuel, Hoffman, Al (1902-1960). Auteur ou responsable intellectuel, Hilger, F.. Auteur ou responsable intellectuel, Barcellini, Frank (1920-2012). Auteur ou responsable intellectuel, Grosbois, Onésime (1927-1964). Piano, Manning, Dick. Auteur ou responsable intellectuel, Hoffman, Al (1902-1960). Auteur ou responsable intellectuel, Hilger, F.. Auteur ou responsable intellectuel, Barcellini, Frank (1920-2012). Auteur ou responsable intellectuel, and Grosbois, Onésime (1927-1964). Piano

Abstract

Titre uniforme : [Hop digui-di], Titre uniforme : [Hop digui-di], Titre uniforme : [Le Facteur de Santa Cruz], BnF-Partenariats, Collection sonore - Believe, Contient une table des matières

Published
1957

31. The complete DNA sequence of yeast chromosome III

Author

Oliver, S. G., Aart, Q. J. M., Agostoni-Carbone, M. L., Aigle, M., Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J. P. G., Benit, P., Berben, G., Bergantino, E., Biteau, N., Bolle, P. A., Bolotin-Fukuhara, M., Brown, A., Brown, A. J. P., Buhler, J. M., Carcano, C., Carignani, G., Cederberg, H., Chanet, R., Contreras, R., Crouzet, M., Daignan-Fornier, B., Defoor, E., Delgado, M., Demolder, J., Doira, C., Dubois, E., Dujon, B., Dusterhoft, A., Erdmann, D., Esteban, M., Fabre, F., Fairhead, C., Faye, G., Feldmann, H., Fiers, W., Francingues-Gaillard, M. C., Franco, L., Frontali, L., Fukuhara, H., Fuller, L. J., Galland, P., Gent, M. E., Gigot, D., Gilliquet, V., Glansdorff, N., Goffeau, A., Grenson, M., Grisanti, P., Grivell, L. A., Haan, M., Haasemann, M., Hatat, D., Hoenicka, J., Hegemann, J., Herbert, C. J., Hilger, F., Hohmann, S., Hollenberg, C. P., Huse, K., Iborra, F., Indge, K. J., Isono, K., Jacq, C., Jacquet, M., James, C. M., Jauniaux, J. C., Jia, Y., Jimenez, A., Kelly, A., Kleinhans, U., Kreisl, P., Lanfranchi, G., Lewis, C., Linden, C. G., Lucchini, G., Lutzenkirchen, K., Maat, M. J., Mallet, L., Mannhaupt, G., Martegani, E., Mathieu, A., Maurer, C. T. C., Mcconnell, D., Mckee, R. A., Messenguy, F., Mewes, H. W., Molemans, F., Montague, M. A., Falconi, M. M., Navas, L., Newlon, C. S., Noone, D., Pallier, C., Panzeri, L., and Pearson, B. M.

32. The nucleotide sequence of Saccharomyces cerevisiae chromosome VII

Author

Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Brückner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., Wergifosse, P., Dujon, B., Durand, P., Entian, K. D., Eraso, P., Escribano, V., Fabiani, L., Fartmann, B., Feroli, F., Feuermann, M., Frontali, L., García-Gonzalez, M., García-Sáez, M. I., Goffeau, A., Guerreiro, P., Hani, J., Hansen, M., Hebling, U., Hernandez, K., Heumann, K., Hilger, F., Hofmann, B., Indge, K. J., James, C. M., Klima, R., Kötter, P., Kramer, B., Kramer, W., Lauquin, G., Leuther, H., Louis, E. J., Maillier, E., Marconi, A., Martegani, E., Mazón, M. J., Mazzoni, C., Mcreynolds, A. D. K., Melchioretto, P., Mewes, H. W., Minenkova, O., Müller-Auer, S., Nawrocki, A., Netter, P., Neu, R., Nombela, C., Oliver, S. G., Panzeri, L., Paoluzi, S., Plevani, P., Portetelle, D., Portillo, F., Potier, S., Purnelle, B., Rieger, M., Riles, L., Rinaldi, T., Robben, J., Rodrigues-Pousada, C., Rodriguez-Belmonte, E., Rodriguez-Torres, A. M., Rose, M., Maurizio RUZZI, Saliola, M., Sánchez-Perez, M., Schäfer, B., Schäfer, M., Scharfe, M., Schmidheini, T., Schreer, A., Skala, J., Souciet, J. L., Steensma, H. Y., Talla, E., Thierry, A., Vandenbol, M., Aart, Q. J. M., and Dyck, L.

33. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X

Author

Galibert, F., Alexandraki, D., Baur, A., Boles, E., Chalwatzis, N., Chuat, Jc, Coster, F., Cziepluch, C., Dehaan, M., Domdey, H., Durand, P., Entian, Kd, Gatius, M., Goffeau, A., Grivell, La, Hennemann, A., Herbert, Cj, Heumann, K., Hilger, F., Hollenberg, Cp, Huang, Me, Jacq, C., Jauniaux, Jc, Katsoulou, C., Kirchrath, L., Kleine, K., Kordes, E., Kotter, P., Liebl, S., Louis, Ej, Manus, V., Mewes, Hw, Miosga, T., Obermaier, B., Perea, J., Pohl, T., Portetelle, D., Aurora Pujol, Purnelle, B., Rad, MR, Rasmussen, Sw, Rose, M., Rossau, R., Schaaffgerstenschlager, I., Smits, Phm, Scarcez, T., Soriano, N., Tovan, D., Tzermia, M., Vanbroekhoven, A., Vandenbol, M., Wedler, H., Wettstein, D., Wambutt, R., Zagulski, M., Zollner, A., and Karpfingerhartl, L.

34. The nucleotide sequence of Saccharomyces cerevisiae chromosome VII

Author

Tettelin, H., Carbone, Mla, Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, Aj, Bruckner, M., Bruschi, Cv, Carignani, G., Castagnoli, L., Cerdan, E., Clemente, Ml, Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Delbino, S., Delius, H., Delneri, D., Dewergifosse, P., Dujon, B., Durand, P., Entian, Kd, Eraso, P., Escribano, V., Fabiani, L., Fartmann, B., Feroli, F., Feuermann, M., Frontali, L., Garciagonzalez, M., Garciasaez, Mi, Goffeau, A., Guerreiro, P., Hani, J., Hansen, M., Hebling, U., Hernandez, K., Heumann, K., Hilger, F., Hofmann, B., Indge, Kj, James, Cm, Klima, R., Kotter, P., Kramer, B., Kramer, W., Lauquin, G., Leuther, H., Louis, Ej, Maillier, E., Marconi, A., Martegani, E., Mazon, Mj, Mazzoni, C., Mcreynolds, Adk, Melchioretto, P., Mewes, Hw, Minenkova, O., Mullerauer, S., Nawrocki, A., Netter, P., Neu, R., César Nombela, Oliver, Sg, Panzeri, L., Paoluzi, S., Plevani, P., Portetelle, D., Portillo, F., Potier, S., Purnelle, B., Rieger, M., Riles, L., Rinaldi, T., Robben, J., Rodriguespousada, C., Rodriguezbelmonte, E., Rodrigueztorres, Am, Rose, M., Ruzzi, M., Saliola, M., Sanchezperez, M., Schafer, B., Schafer, M., Scharfe, M., Schmidhelni, T., Schreer, A., Skala, J., Souciet, Jl, Steensma, Hy, Talla, E., Thierry, A., Vandenbol, M., Vanderaart, Qjm, Vandyck, L., Vanoni, M., Verhasselt, P., Voet, M., Volckaert, G., Wambutt, R., Watson, Md, Weber, N., Wedler, E., Wedler, H., Wipfli, P., Wolf, K., Wright, Lf, Zaccaria, P., Zimmermann, M., Zollner, A., and Kleine, K.

Subjects
Multidisciplinary

46. Large-scale phenotypic analysis reveals identical contributions to cell functions of known and unknown yeast genes.

Author

Bianchi MM, Ngo S, Vandenbol M, Sartori G, Morlupi A, Ricci C, Stefani S, Morlino GB, Hilger F, Carignani G, Slonimski PP, and Frontali L

Subjects
Gene Deletion, Genetic Linkage genetics, Genetic Linkage physiology, Open Reading Frames genetics, Open Reading Frames physiology, Phenotype, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae physiology, Genes, Fungal physiology, Genome, Fungal, Saccharomyces cerevisiae genetics
Abstract

Sequencing of the yeast genome has shown that about one-third of the yeast ORFs code for unknown proteins. Many other have similarity to known genes, but still the cellular functions of the gene products are unknown. The aim of the B1 Consortium of the EUROFAN project was to perform a qualitative phenotypic analysis on yeast strains deleted for functionally orphan genes. To this end we set up a simple approach to detect growth defects of a relatively large number of strains in the presence of osmolytes, ethanol, high temperature, inhibitory compounds or drugs affecting protein biosynthesis, phosphorylation level or nucleic acids biosynthesis. We have now developed this procedure to a semi-quantitative level, we have included new inhibitors, such as hygromycin B, benomyl, metals and additional drugs interfering with synthesis of nucleic acids, and we have performed phenotypic analysis on the deleted strains of 564 genes poorly characterized in respect to their cellular functions. About 30% of the deleted strains showed at least one phenotype: many of them were pleiotropic. For many gene deletions, the linkage between the deletion marker and the observed phenotype(s) was studied by tetrad analysis and their co-segregation was demonstrated. Co-segregation was found in about two-thirds of the analysed strains showing phenotype(s)., (Copyright 2001 John Wiley & Sons, Ltd.)

Published
2001
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47. The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.

Author

Johnston M, Hillier L, Riles L, Albermann K, André B, Ansorge W, Benes V, Brückner M, Delius H, Dubois E, Düsterhöft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, Kötter P, Louis EJ, Messenguy F, Mewes HW, and Hoheisel JD

Subjects
Base Sequence, DNA, Fungal, Molecular Sequence Data, Chromosomes, Fungal, Saccharomyces cerevisiae genetics
Abstract

The yeast Saccharomyces cerevisiae is the pre-eminent organism for the study of basic functions of eukaryotic cells. All of the genes of this simple eukaryotic cell have recently been revealed by an international collaborative effort to determine the complete DNA sequence of its nuclear genome. Here we describe some of the features of chromosome XII.

Published
1997

48. The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII reveals the presence of eight open reading frames.

Author

Vandenbol M, Durand P, Portetelle D, and Hilger F

Subjects
Adenosine Deaminase genetics, Amino Acid Sequence, Amino Acyl-tRNA Synthetases genetics, Animals, Ankyrins genetics, Fungal Proteins genetics, Genes, Fungal, Humans, Molecular Sequence Data, RNA-Binding Proteins, Sequence Homology, Amino Acid, Chromosomes, Fungal genetics, DNA, Fungal genetics, Open Reading Frames, Saccharomyces cerevisiae genetics
Abstract

The complete sequence of a 11, 132 bp segment located on the left arm of chromosome VII of Saccharomyces cerevisiae has been determined and analysed. Eight open reading frames (ORFs) of at least 100 amino acids were identified. Five show similarities to known amino acid sequences. Another ORF encoding the chromosome segregation protein CSE1 is not entirely located in our sequenced fragment and is incomplete at its C-terminus. The two remaining ORFs do not display similarities to known sequences.

Published
1995
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49. Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Tyl-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element.

Author

Vandenbol M, Durand P, Portetelle D, and Hilger F

Subjects
Amino Acid Sequence, DNA Transposable Elements genetics, Frameshifting, Ribosomal genetics, Genes, Suppressor genetics, Humans, Molecular Sequence Data, Open Reading Frames genetics, RNA, Transfer, Gly genetics, RNA, Transfer, Thr genetics, Retroelements genetics, Sequence Homology, Amino Acid, Chromosomes, Fungal, Genes, Fungal genetics, Saccharomyces cerevisiae genetics, Sequence Analysis, DNA
Abstract

We have sequenced on both strands a 44,019 bp fragment located on the left arm of Saccharomyces cerevisiae chromosome XV. The sequenced segment contains 22 open reading frames (ORFs) of at least 100 amino acids long, one of which probably contains an intron. Six of the 22 ORFs correspond to known proteins: the multicopy suppressor of Snf1 protein 1, the two Tyl-H3 transposon proteins TyA and TyB, the myo-inositol transporter 2, the transcription factor protein Ino4 and the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase. Of the 16 remaining ORFs, two show highest homologies with the yeast serine/threonine protein kinase Ste20 and the human tryptophanyl-tRNA synthetase. Eight ORFs show slight similarities with protein sequences described in data banks. DNA sequence comparison reveals also the presence of three known sequences: the Tyl-H3 transposable element, the yeast suf1(+) frameshift suppressor gene for tRNA-Gly and the yeast transfer RNA-Thr-1a. A fourth DNA sequence shows striking identities with the yeast delta elements.

Published
1995
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50. Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8.

Author

Vandenbol M, Durand P, Dion C, Portetelle D, and Hilger F

Subjects
Carboxylic Ester Hydrolases chemistry, Carboxylic Ester Hydrolases genetics, Chromosome Mapping, DNA-Directed DNA Polymerase chemistry, DNA-Directed DNA Polymerase genetics, Lipase chemistry, Lipase genetics, Molecular Sequence Data, Open Reading Frames genetics, Sequence Analysis, Sequence Analysis, DNA, Sequence Homology, Carboxylesterase, Chromosomes, Fungal genetics, DNA, Fungal genetics, Ribosomal Proteins genetics, Saccharomyces cerevisiae genetics
Abstract

We have sequenced a continuous segment of 17,137 bp on chromosome X. Sequence analysis of this stretch revealed 14 open reading frames (ORFs) at least 100 amino acids long. One gene, encoding the mitochondrial 60S ribosomal protein L8, had already been sequenced. Four ORF products show weak homologies with known protein sequences. The nine remaining ORF products have no homologies with sequences in data banks.

Published
1995
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