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The premise of research in human physiology is to explore a multifaceted system whilst identifying one or a few outcomes of interest. Therefore, the control of potentially confounding variables requires careful thought regarding the extent of control and complexity of standardisation. One common factor to control prior to testing is diet, as food and fluid provision may deviate from participants' habitual diets, yet a self-report and replication method can be flawed by under-reporting. Researchers may also need to consider standardisation of physical activity, whether it be through familiarisation trials, wash-out periods, or guidance on levels of physical activity to be achieved before trials. In terms of pharmacological agents, the ethical implications of standardisation require researchers to carefully consider how medications, caffeine consumption and oral contraceptive prescriptions may affect the study. For research in females, it should be considered whether standardisation between- or within-participants in regards to menstrual cycle phase is most relevant. The timing of measurements relative to various other daily events is relevant to all physiological research and so it can be important to standardise when measurements are made. This review summarises the areas of standardisation which we hope will be considered useful to anyone involved in human physiology research, including when and how one can apply standardisation to various contexts. [ABSTRACT FROM AUTHOR]

Spatial thinking is an integral skill for geography students to develop. Whilst many spatial competencies have been identified by researchers, and the merits of GIS seemingly ubiquitous in the published literature, little work has been done into how students' spatial thinking skills can be assessed. Therefore, further investigation into the relationship between spatial thinking and performance and attainment is needed. This research investigates the impact using a geographic information system (GIS) has on students' spatial thinking skills and attempts to assess the extent using a framework. This was done through the design and implementation of two GIS-based interventions. This small-scale evaluation used qualitative methods to investigate students' and teachers' views. Student work was also analysed using the framework developed for the assessment of spatial thinking skills. The findings suggest that the use of a GIS does enhance, and in most cases improves students' spatial thinking skills, but, that spatial thinking is hard to quantify and difficult to measure progress in. Another benefit that using a GIS affords is the creation of engaging, contemporary and interactive lessons, using real data, from which students derive a lot of geographical value.

This critical review aims to highlight how modeling of the immune response has adapted over time to utilize microphysiological systems. Topics covered here will discuss the integral components of the immune system in various human body systems, and how these interactions are modeled using these systems. Through the use of microphysiological systems, we have not only expanded on foundations of basic immune cell information, but have also gleaned insight on how immune cells work both independently and collaboratively within an entire human body system. [ABSTRACT FROM AUTHOR]

Opioid overdose is the leading cause of drug overdose lethality, posing an urgent need for investigation. The key brain region for inspiratory rhythm regulation and opioid‐induced respiratory depression (OIRD) is the preBötzinger Complex (preBötC) and current knowledge has mainly been obtained from animal systems. This study aims to establish a protocol to generate human preBötC neurons from induced pluripotent cells (iPSCs) and develop an opioid overdose and recovery model utilizing these iPSC‐preBötC neurons. A de novo protocol to differentiate preBötC‐like neurons from human iPSCs is established. These neurons express essential preBötC markers analyzed by immunocytochemistry and demonstrate expected electrophysiological responses to preBötC modulators analyzed by patch clamp electrophysiology. The correlation of the specific biomarkers and function analysis strongly suggests a preBötC‐like phenotype. Moreover, the dose‐dependent inhibition of these neurons' activity is demonstrated for four different opioids with identified IC50's comparable to the literature. Inhibition is rescued by naloxone in a concentration‐dependent manner. This iPSC‐preBötC mimic is crucial for investigating OIRD and combating the overdose crisis and a first step for the integration of a functional overdose model into microphysiological systems. [ABSTRACT FROM AUTHOR]

Spatial thinking is an integral skill for geography students to develop. Whilst many spatial competencies have been identified by researchers, and the merits of GIS seemingly ubiquitous in the published literature, little work has been done into how students' spatial thinking skills can be assessed. Therefore, further investigation into the relationship between spatial thinking and performance and attainment is needed. This research investigates the impact using a geographic information system (GIS) has on students' spatial thinking skills and attempts to assess the extent using a framework. This was done through the design and implementation of two GIS-based interventions. This small-scale evaluation used qualitative methods to investigate students' and teachers' views. Student work was also analysed using the framework developed for the assessment of spatial thinking skills. The findings suggest that the use of a GIS does enhance, and in most cases improves students' spatial thinking skills, but, that spatial thinking is hard to quantify and difficult to measure progress in. Another benefit that using a GIS affords is the creation of engaging, contemporary and interactive lessons, using real data, from which students derive a lot of geographical value. [ABSTRACT FROM AUTHOR]