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Oxygen reduction reaction (ORR) plays a crucial role in electrochemical energy conversion and storage devices such as metal–air batteries and water electrolyzers.

Endogenous hydrogen peroxide (H2O2) is involved in regulating the gibberellic acid-induced programmed cell death (PCD) of the aleurone layers by cooperating with OsVPE3 during rice seed germination. Preliminary experiments revealed that H2O2 produced by the NOX pathway is the key factor affecting rice germination. Histochemical analysis indicated that H2O2 is located in the aleurone layer. Both the H2O2 scavenger DMTU and the NOX inhibitor DPI decreased H2O2 content and significantly slowed down vacuolation in a dose-dependent manner. Interestingly, DMTU down-regulated the OsNOX8 transcript or DMTU and DPI decreased the intracellular H2O2 level, resulting in a delay of PCD. In contrast, GA and H2O2 up-regulated the OsNOX8 transcript and intracellular H2O2 level, leading to premature PCD, and the effects of GA and H2O2 were reversed by DMTU and DPI, respectively. These results showed that the imbalance of intracellular H2O2 levels leads to the delayed or premature PCD. Further experiments indicated that GA up-regulated the OsVPE3 transcript and VPE activity, and the effect was reversed by DPI. Furthermore, Ac-YVAD-CMK significantly blocked H2O2 accumulation, and DPI + Ac-YVAD-CMK had a more significant inhibitory effect compared with DPI alone, resulting in the delayed PCD, suggesting that OsVPE3 regulates PCD by promoting H2O2 generation. Meanwhile, DPI significantly inhibited the OsVPE3 transcript and VPE activity, and in turn delayed PCD occurrence, suggesting that the H2O2 produced by the NOX pathway may regulate PCD by up-regulating the OsVPE3 transcript. Thus, the endogenous H2O2 produced by the NOX pathway mediates the GA-induced PCD of rice aleurone layers by interacting with OsVPE3.

This paper concerns a class of local and nonlocal diffusion systems with double free boundaries possessing different moving parameters. We firstly obtain the existence, uniqueness and regularity of global solution and then prove that its dynamics are governed by a spreading-vanishing dichotomy. Then the sharp criteria for spreading and vanishing are established. Of particular importance is that long-time behaviors of solution in this problem are quite rich under the Lotka-Volterra type competition, prey-predator and mutualist growth conditions. Moreover, we also provide rough estimates of spreading speeds when spreading happens.

This paper investigates the problem of sliding mode control (SMC) for a class of uncertain nonlinear Markovian jump systems through T-S fuzzy models. By adopting some convexification techniques, new results on stochastic stability analysis of the sliding motion are attained. Then two new SMC design approaches are proposed to force the closed-loop system states onto the sliding surface in finite time. Finally, two simulation examples are shown to verify the effectiveness of the proposed approaches.

Metaphor is the central issue of language and thinking. Metaphor identification plays a significant preliminary role in the field of machine translation, reading comprehension, and automatic summarization, making it a focus of natural language processing. Recently, research into Chinese verb metaphor identification has become a widespread concern. The main problem is that the usage of semantic resources is relatively simple, and there is a lack of deep semantic support. Therefore, this paper proposes a word representation method suitable for metaphor classification tasks, which combines the traditional word vector with structural information from the Synonym Thesaurus, so that the word vector can contain the abstraction degree of the word in the metaphor. On this basis, we propose a verb metaphor attention network based on subject-predicate and verb-object relationships, which gives full consideration to the global syntactic information when we perform LSTM encoding and calculate the weight of each word. At the same time, it can facilitate the understanding of literalness and non-literalness. The experimental results show that the identification effect improves on the existing results, indicating that word representation combining semantic resources and attention network can improve the verb metaphor identification performance.

Endogenous hydrogen peroxide (H

Background Vacuolar processing enzymes (VPEs) have been identified as the enzymes that regulate vacuole-mediated programmed cell death (PCD) in plants. The mechanism that VPE regulates the PCD in rice aleurone layers remains unknown. Results The aleurone layers treated with distilled water exerted caspase-1 and VPE activity, both of which were inhibited by the caspase-1 specific inhibitor Ac-YVAD-CMK but not by the caspase-3 specific inhibitor Ac-DEVD-CHO. However, the caspase-1 and caspase-3 inhibitors weakened the activity of caspase-3. Combined with the effects of endogenous gibberellin (GA) on the induction of OsVPEs, we suggest that the OsVPE3 in the aleurone layers, which exhibits caspase-1-like activity, is a key molecule in GA-induced PCD via regulating the protease with caspase-3-like activity. Many studies have confirmed that vacuolar fusion is an important feature of vacuole-mediated PCD in plants. In this experiment, the process of vacuole fusion was accompanied by changes in the structure of actin filaments (AFs), specifically, their depolymerization and polymerization. The process of vacuolar fusion was accelerated or delayed by the promotion or inhibition of the depolymerization of AFs, respectively. Here, the inhibition of OsVPE3 blocked the depolymerization of AFs and delayed the fusion of vacuoles, indicating that OsVPE3 can regulate the fusion of vacuoles in rice aleurone layers via mediating AFs. Furthermore, the depolymerization of AFs contributed to the up-regulation of OsVPE3 gene expression and VPE activity, resulting in accelerated PCD in rice aleurone layers. However, the inhibitor of VPE reversed the effects of AF depolymerization on the activity of VPE, then postponing the process of PCD, implying that AF can involve in GA-induced PCD of rice aleurone layers by mediating OsVPE3. Conclusions Together, activation of OsVPE3 and depolymerization of AFs shortened the process of vacuolation and PCD in rice aleurone layers, and OsVPE3 interacted with AFs during regulation.

This paper designs a robust output feedback controller for a class of T-S fuzzy affine impulsive systems. Through a descriptor-system-based scheme and based on piecewise quadratic Lyapunov functions, sufficient conditions for exponential stability of the resulting closed-loop system are obtained, and the controller gains can be acquired by solving a set of linear matrix inequalities. A simulation example is given to show the validity of the proposed approach.

Humor plays important role in human communication, which makes it important problem for natural language processing. Prior work on the analysis of humor focuses on whether text is humorous or not, or the degree of funniness, but this is insufficient to explain why it is funny. We therefore create a dataset on humor with 9,123 manually annotated jokes in Chinese. We propose a novel annotation scheme to give scenarios of how humor arises in text. Specifically, our annotations of linguistic humor not only contain the degree of funniness, like previous work, but they also contain key words that trigger humor as well as character relationship, scene, and humor categories. We report reasonable agreement between annota-tors. We also conduct an analysis and exploration of the dataset. To the best of our knowledge, we are the first to approach humor annotation for exploring the underlying mechanism of the use of humor, which may contribute to a significantly deeper analysis of humor. We also contribute with a scarce and valuable dataset, which we will release publicly.

In a resource-limited environment, we propose and study an epidemic model consisting of two groups of hosts to reflect the different risk levels of becoming infected and transmitting infection. To further investigate vaccine implementation policies, we study the global dynamics of the model with group-dependent constant coverage rates by utilizing Lyapunov functions. We find that when the vaccination-dependent basic reproduction number is less than one (ℜ0 1, the endemic equilibrium is globally attractive. For the case when the disease is endemic, we apply the optimal control theory to the model under the assumption of a resource-constrained environment and we derive optimal coverage strategies. We also perform numerical simulations for the optimal control problem to verify and further support the theoretical results. In limited control resource cases with both constant and variable coverage rate strategies, we show that when the total control resources are rather scarce, the resources should be biased towards at the group with the high level of infection risk; and that the control resources should preferably be directed at the lower risk group if the total resources are relatively abundant.

This paper studies the robust output feedback control problem for a class of nonlinear systems with impulsive effects via T-S fuzzy affine models. By employing a state-input augmentation scheme, some new results on the piecewise affine (PWA) output feedback controller synthesis for T-S fuzzy affine impulsive systems are obtained through piecewise quadratic Lyapunov functions. Sufficient criteria for exponential stability analysis of the resulting closed-loop system are given within a convex optimization setup. A simulation example is shown to verify the effectiveness of the proposed approach.

Hydrogen peroxide (H2O2) is a reactive oxygen species (ROS) that plays a dual role in plant cells. Here, we discovered that drought (20% polyethylene glycol-6000, PEG)-triggered decreases of HO-1 transcript expression and HO activity. However, exogenous H2O2 contributed toward the increase in HO-1 gene expression and activity of the enzyme under drought stress. Meanwhile, the HO-1 inducer hematin could mimic the effects of the H2O2 scavengers ascorbic acid (AsA) and dimethylthiourea (DMTU) and the H2O2 synthesis inhibitor diphenyleneiodonium (DPI) for scavenging or diminishing drought-induced endogenous H2O2. Conversely, the zinc protoporphyrin IX (ZnPPIX), an HO-1-specific inhibitor, reversed the effects of hematin. We further analyzed the endogenous H2O2 levels and HO-1 transcript expression levels of aleurone layers treated with AsA, DMTU, and DPI in the presence of exogenous H2O2 under drought stress, respectively. The results showed that in aleurone layers subjected to drought stress, when the endogenous H2O2 level was inhibited, the effect of exogenous H2O2 on the induction of HO-1 was enhanced. Furthermore, exogenous H2O2-activated HO-1 effectively enhanced amylase activity. Application of 8-bromoguanosine 3',5'-cyclic guanosine monophosphate (8-Br-cGMP) (the membrane permeable cGMP analog) promoted the effect of exogenous H2O2-delayed PCD of aleurone layers in response to drought stress. More importantly, HO-1 delayed the programmed cell death (PCD) of aleurone layers by cooperating with nitric oxide (NO), and the delayed effect of NO on PCD was achieved via mediation by cGMP under drought stress. In short, in rice aleurone layers, exogenous H2O2 (as a signaling molecule) triggered HO-1 and delayed PCD via cGMP which possibly induced amylase activity under drought stress. In contrast, as a toxic by-product of cellular metabolism, the drought-generated H2O2 promoted cell death.

Vacuole fusion is a necessary process for the establishment of a large central vacuole, which is the central location of various hydrolytic enzymes and other factors involved in death at the beginning of plant programmed cell death (PCD). In our report, the fusion of vacuoles has been presented in two ways: i) small vacuoles coalesce to form larger vacuoles through membrane fusion, and ii) larger vacuoles combine with small vacuoles when small vacuoles embed into larger vacuoles. Regardless of how fusion occurs, a large central vacuole is formed in rice (Oryza sativa) aleurone cells. Along with the development of vacuolation, the rupture of the large central vacuole leads to the loss of the intact plasma membrane and the degradation of the nucleus, resulting in cell death. Stabilizing or disrupting the structure of actin filaments (AFs) inhibits or promotes the fusion of vacuoles, which delays or induces PCD. In addition, the inhibitors of the vacuolar processing enzyme (VPE) and cathepsin B (CathB) block the occurrence of the large central vacuole and delay the progression of PCD in rice aleurone layers. Overall, our findings provide further evidence for the rupture of the large central vacuole triggering the PCD in aleruone layers.

Cereal aleurone layers undergo a gibberellin (GA)-regulated process of programmed cell death (PCD) following germination. Heme oxygenase-1 (HO-1) is known as a rate-liming enzyme in the degradation of heme to biliverdin IXα (BV), carbon monoxide (CO), and free iron ions (Fe2+). It is a critical component in plant development and adaptation to environment stresses. Our previous studies confirmed that HO-1 inducer hematin (Ht) promotes the germination of rice seeds in drought (20% polyethylene glycol-6000, PEG) conditions, but the corresponding effects of HO-1 on the alleviation of germination-triggered PCD in GA-treated rice aleurone layers remain unknown. The present study has determined that GA co-treated with PEG results in lower HO-1 transcript levels and HO activity, which in turn results in the development of vacuoles in aleurone cells, followed by PCD. The pharmacology approach illustrated that up- or down-regulated HO-1 gene expression and HO activity delayed or accelerated GA-induced PCD. Furthermore, the application of the HO-1 inducer hematin and nitric oxide (NO) donor sodium nitroprusside (SNP) not only activated HO-1 gene expression, HO activity, and endogenous NO content, but also blocked GA-induced rapid vacuolation and accelerated aleurone layers PCD under drought stress. However, both HO-1 inhibitor zinc protoporphyrin IX (ZnPPIX) and NO scavenger 2-(4-carboxyphenyl0-4, 4, 5, 5-tetramethylimidazoline-l-oxyl-3-oxide potassium salt (cPTIO) reserved the effects of hematin and SNP on rice aleurone layer PCD under drought stress by down-regulating endogenous HO-1 and NO, respectively. The inducible effects of hematin and SNP on HO-1 gene expression, HO activity, and NO content were blocked by cPTIO. Together, these results clearly suggest that HO-1 is involved in the alleviation of GA-induced PCD of drought-triggered rice aleurone layers by associating with NO.